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394 results about "Plate method" patented technology

Bacillus amyloliquefaciens D2WM as well as preparation method and application of bacillus amyloliquefaciens D2WM

The invention discloses a bacillus amyloliquefaciens D2WM as well as a preparation method and application of bacillus amyloliquefaciens D2WM. The bacillus amyloliquefaciens D2WM comprises the steps of A, bacillus separation and purification, namely collecting after inoculating a slope; B, preliminary screening of bacillus, namely sequentially selecting strains having antagonistic effects on Erwinia and camellia oleifera diseases by using a point connection method and a plate confrontation method; and C, secondary screening of bacillus, namely culturing the preliminarily-screened strains with the antagonistic effects in an LB liquid culture medium by using a cup-plate method, and screening the strains simultaneously having the antagonistic effects on main diseases of Erwinia and camellia oleifera through an antagonistic bacterium fermentation liquid and sterile filtrate. When being applied to preparation of drugs for treating or preventing soft rot of vegetables and camellia oleifera diseases, the bacillus amyloliquefaciens has a broad-spectrum antagonistic effect, high in growth speed, capable of efficiently antagonizing anthracnose of camellia oleifera, leaf spot diseases of camellia oleifera, black spot of camellia oleifera, canker of camellia oleifera and various diseases and remarkable in antibacterial effect, the culture time for general bacteria and fungi is greatly shortened, and the production cost is reduced.
Owner:HUBEI ENG UNIV

Reservoir capillary pressure curve determinator under high temperature and pressure

InactiveCN102621034AStable pressureSolve the problem of large errorsMaterial analysisPressure curveFluid saturation
The invention provides a reservoir capillary pressure curve determinator under a high temperature and a pressure. The determinator comprises a ring pressure system, an injection system, a core clamping system, a semi-permeable baffle plate, a back pressure system, a measuring system, a data automatically collecting and processing system, and a thermostat which are connected through pipelines. A semi-permeable baffle plate method is adopted to measure capillary pressure curves under a stratum condition, and the basic rationale is that a principle of balance between an impressed pressure and the capillary pressure is used to measure the capillary pressure curves under the high temperature and the pressure. Core saturated wetting phase fluid displaces a wetting phase with a nonwetting phase under a certain displacement pressure. When the impressed pressure overcomes the capillary pressure of a certain capillary in the core, the wetting phase fluid in the capillary is discharged gradually, and the wetting phase fluid saturation in the core is reduced. Pressure is increased stepwise, accumulated discharged quantity of the wetting phase fluid when the wetting phase fluid is no longer discharged and an injection pressure are measured, and the capillary pressure curves can be solved.
Owner:CHINA UNIV OF PETROLEUM (EAST CHINA)

Two-dimensional visualized test device of work mechanism of anchoring system

The invention relates to a two-dimensional visualized test device of the work mechanism of an anchoring system, belonging to the technical field of geotechnical engineering. The two-dimensional visualized test device is formed by combining a left-side loading plate (5), a top loading plate (6), a right-side fixed plate (11), a bottom plate (22), a camera device (20), and the like. According to the two-dimensional visualized test device, a sample chamber (10) of is formed by adopting a staggered plate method, and therefore, the problem of blank corner of an anchoring body is solved; the camera device (20) is arranged so as to shoot the behavioral process of forming the anchoring system by an anchor rod, an anchoring agent and the anchoring body in the overall drawing process of the anchor rod in a whole course, and the behavioral process can be directly observed by eyes, and therefore, the limitation of inconvenient monitoring and observation of the mechanical response of the anchoring system in the traditional three-dimensional test is overcome; and the test basis of the research of the work mechanism of the anchoring system is provided. The invention can be widely used for the test research of a full-length bond-type anchor rod and the mechanical behavior and the work mechanism of the corresponding anchoring system in geotechnical engineering.
Owner:INST OF ROCK AND SOIL MECHANICS - CHINESE ACAD OF SCI

Preparation method of heavy metal polluted soil repairing agent

InactiveCN106833674AEasy to fixShield vicious competitionBacteriaOther chemical processesMicroorganismHydrogen
The invention relates to the technical field of soil repairing and in particular to a preparation method of a heavy metal polluted soil repairing agent. The repairing agent is composed of a microorganism and expanded vermiculite; the preparation method comprises the following steps: after carrying out sterile treatment on the expanded vermiculite, mixing the expanded vermiculite and a bacterium suspension solution together according to a ratio; then carrying out adsorption culture on a mixture in a dark place; after few hours, carrying out adsorption balance and carrying out immobilization of the microorganism; providing suitable culture conditions for the microorganism by the bacterium suspension solution according to different conditions on nutrients, the potential of hydrogen, oxygen and the like of the microorganism; or adding an inhibitor to cause an environment which is only good for the growth of the microorganism and is used for inhibiting the growth of other microorganisms, so as to eliminate other microorganisms which are not needed; separating and purifying the microorganism by adopting a diluting and coating flat plate method or a flat plate marking method until a pure strain is obtained; inoculating the purified strain into a culture solution and culturing to obtain the heavy metal polluted soil repairing agent. The preparation method provided by the invention is simple and low in investment cost; the repairing agent can be used for effectively improving an immobilization effect of the microorganism on heavy metal and is suitable for repairing low-concentration heavy metal polluted farmland soil.
Owner:东莞市生态环境技术中心
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