Preparation method of heavy metal polluted soil repairing agent

A technology of polluted soil and remediation agent, applied in the field of soil remediation, can solve the problems of large project scale and large investment funds, achieve low investment and operation costs, low investment costs, and improve the effect of immobilization

Inactive Publication Date: 2017-06-13
东莞市生态环境技术中心
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Due to the wide range of heavy metal pollution and the characteristics of its industrial use, industrial sites often use large-scale engineering technology methods for restoration, which requires a lot of investment and large-scale projects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0032] 1. Source of bacteria:

[0033] Bacteria cultured by biochemical treatment of activated sludge from comprehensive wastewater in an electroplating industrial park were activated in a transfer slope and liquid medium, and stored in a refrigerator at 4°C.

[0034] 2. Medium:

[0035] (1) Liquid medium: beef extract 3g, peptone 5g, NaCl 2.5g, distilled water 1000mL.

[0036] (2) Solid medium: beef extract 3g, peptone 5g, NaCl 2.5g, agar 15g, distilled water 15g.

[0037] (3) Screening medium: beef extract 3g, peptone 5g, NaCl 2.5g, distilled water 1000mL, add a certain concentration of cadmium and copper solution.

[0038] 3. Acclimatization, isolation and purification of strains:

[0039] (1) Preparation of soil suspension:

[0040] Take 10g of the mud sample and place it in a conical flask filled with 90mL of sterile water, vibrate with a shaker, and dilute the soil suspension to 10 -3 、10 -4 、10 -5 spare.

[0041] (2) Water sample dilution:

[0042] Take the soi...

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PUM

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Abstract

The invention relates to the technical field of soil repairing and in particular to a preparation method of a heavy metal polluted soil repairing agent. The repairing agent is composed of a microorganism and expanded vermiculite; the preparation method comprises the following steps: after carrying out sterile treatment on the expanded vermiculite, mixing the expanded vermiculite and a bacterium suspension solution together according to a ratio; then carrying out adsorption culture on a mixture in a dark place; after few hours, carrying out adsorption balance and carrying out immobilization of the microorganism; providing suitable culture conditions for the microorganism by the bacterium suspension solution according to different conditions on nutrients, the potential of hydrogen, oxygen and the like of the microorganism; or adding an inhibitor to cause an environment which is only good for the growth of the microorganism and is used for inhibiting the growth of other microorganisms, so as to eliminate other microorganisms which are not needed; separating and purifying the microorganism by adopting a diluting and coating flat plate method or a flat plate marking method until a pure strain is obtained; inoculating the purified strain into a culture solution and culturing to obtain the heavy metal polluted soil repairing agent. The preparation method provided by the invention is simple and low in investment cost; the repairing agent can be used for effectively improving an immobilization effect of the microorganism on heavy metal and is suitable for repairing low-concentration heavy metal polluted farmland soil.

Description

technical field [0001] The invention relates to the technical field of soil remediation, in particular to a method for preparing a remediation agent for low-concentration heavy metal pollution treatment of farmland soil. Background technique [0002] According to incomplete statistics, the area of ​​cultivated land polluted by heavy metals in China is about 2.0×107 hm 2 , accounting for about 20% of the total cultivated land area, mainly with moderate and light pollution. The sources of heavy metals in the soil are mainly the release of acid mines, the discharge of waste water and residues from mining and smelting industries, and other sources include sewage irrigation and sludge abuse, excessive application of pesticides and chemical fertilizers, and incomplete combustion of fossil fuels. The form of heavy metals in the soil affects their activity, bioavailability and migration characteristics. Some forms of heavy metals can be absorbed by plants, and eventually enter the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/40C12N1/20C12N1/02C12R1/07C09K109/00
CPCC09K17/40C09K2109/00C12N1/02C12N1/20
Inventor 邓杰帆黎雯曾彩明王书杰何觉聪谭伟红张文心
Owner 东莞市生态环境技术中心
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