453results about "Microorganism separation" patented technology

The present invention provides a method for producing extracellular vesicles, the method including a step for passing a crushed liquid of plants, mushrooms, or microalgae through a depth filter, and a step for subjecting the crushed liquid after passing through the depth filter to ultrafiltration treatment, the depth filter being composed of filaments.

The invention discloses a bacillus subtilis strain and application thereof in prevention and treatment of tomato brown wrinkled fruit virus diseases. According to the bacillus subtilis strain, the bacillus subtilis strain is NA25, the preservation name is bacillus subtilis, the bacillus subtilis strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), the address of the preservation unit is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation date is April 27, 2025; the preservation number of the strain is CGMCC No. 34380. The bacillus subtilis provided by the invention is separated from healthy tomato endophyte, and the strain fermentation liquor and cell suspension both have a good inhibition effect on the brown wrinkled fruit virus, and can be used for preventing and treating the tomato brown wrinkled fruit virus.

The embodiment of the invention discloses a Yarrowia guilliermondii as well as a separation and screening method, an identification method and application of the Yarrowia guilliermondii. The preservation number of the Yarrowia guilliermondii is CGMCC (China General Microbiological Culture Collection Center) No.27603, and the preservation number of the Yarrowia guilliermondii is CGMCC No.27603. The Yarrowia guilliermondii disclosed by the embodiment of the invention has a very good prevention and control effect on postharvest diseases of fruits, and the prevention and control effect of the Yarrowia guilliermondii on the diseases can be enhanced by respectively adding auxiliary factors, namely calcium chloride, alginate oligosaccharide (AOS), phytic acid (PA) and metschhia yeast. Through identification, the antagonistic bacterium is a Yeyrozyma guilliermondii, and the antagonistic bacterium is a Yeyrozyma guilliermondii. The M.guilliermonii has a good control effect on gray mold, penicilliosis, black spot and aspergillus niger of strawberry, apple, pear and tomato fruits, can be rapidly colonized on fruit wounds, and can enhance the resistance-related enzyme activity of the fruits, occupy the wound space and induce the fruit resistance to reduce the morbidity of diseases.

The invention belongs to the field of microorganisms, and particularly relates to bacillus Z161 capable of efficiently degrading straw as well as a screening method and application of the bacillus Z161. The bacillus subtilis Z161 is bacillus mexicanus, the preservation number of the bacillus mexicanus Z161 is CGMCC (China General Microbiological Culture Collection Center) NO.36393, and the bacillus mexicanus Z161 is preserved in the China General Microbiological Culture Collection Center on October 29, 2025. The bacillus Z161 screened by the invention has strong laccase activity and high temperature resistance, and can efficiently degrade lignin under the condition of 50 DEG C; the problem that in the prior art, normal-temperature zymophyte is mostly adopted, and the requirement for fermentation conditions in the fermentation process is harsh is solved, and therefore the rapid compost fermentation technology is easier to achieve. The bacillus Z161 provided by the invention has the advantages of genetic and functional character stability, has good tolerance at high temperature, enables lignin to be quickly depolymerized, and improves the decomposition efficiency.

The invention relates to a gastric acid-resistant sustained-release intestinal bacteria capsule based on bionic mucous membrane-enzymolysis response and a preparation method thereof. The gastric acid-resistant sustained-release intestinal bacteria capsule comprises a core layer, an adhesive layer, an outer-layer composite film and a protective coating from inside to outside, the core layer consists of intestinal flora and a multifunctional freeze-drying protective agent; the adhesive layer is composed of trehalose, N-acetyl glucosamine and MUC2 recombinant protein; the outer-layer composite film is composed of a base material and a functional filler; the protective coating is composed of beewax and chitosan. The anti-gastric acid sustained-release intestinal bacteria capsule based on bionic mucosa-enzymolysis response provided by the invention has excellent gastric acid resistance, and the gastric acid resistance is remarkably improved; good slow release performance is achieved, and accurate slow release of enzymatic hydrolysis response can be achieved; the colonization efficiency of the intestinal bacteria can be effectively improved; the storage stability is good. The preparation method provided by the invention has the advantages of simplified steps, short time consumption, controllable core operation time and obviously shortened whole process, and is beneficial to keeping the activity of the flora.

The invention relates to selenium-enriched pediococcus acidilactici Se34 with anti-oxidation, cholesterol-lowering and anti-tumor effects and application of the selenium-enriched pediococcus acidilactici Se34. The pediococcus acidilactici Se34 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number of the pediococcus acidilactici Se34 is CGMCC No.33933, and the preservation date of the pediococcus acidilactici Se34 is March 24, 2025. The pediococcus acidilactici Se34 provided by the invention integrates excellent probiotic characteristics (acid-resistant bile salt, safe and colonizable), efficient selenium enrichment capacity and remarkably enhanced activity of resisting oxidation, reducing cholesterol and inhibiting specific cancer cell proliferation and metastasis after selenium enrichment. The strain and the selenium-rich product thereof have important application value in the fields of developing functional foods (selenium-rich probiotic products), antioxidant / cholesterol-reducing health-care foods, potential auxiliary anticancer biological preparations or prodrugs and the like.

The invention discloses a preparation method of engineered bacteria for releasing CO gas under initiation of ultrasonic waves to kill tumor cells, and relates to a preparation method of CO carrier engineered bacteria. The invention aims to solve the problems that the existing CO carrier material is low in delivery efficiency, the CO is difficult to release efficiently and controllably in space and time at the same time, and the existing metal CO carrier material is high in toxicity. Meanwhile, the problems that the penetrating power of light waves to biological tissues is limited, and the CO release efficiency is limited due to a tumor hypoxic microenvironment are solved. The preparation method comprises the following steps: 1, modifying the surface of escherichia coli with 3-hydroxyflavone-D-alanine; and 2, continuously modifying the surface of the escherichia coli with CaO2 (at) polymyxin B. The method is used for preparing the engineered bacteria for releasing the CO gas through ultrasonic initiation to kill the tumor cells.

This invention relates to the Kluyveromyces marxianus strain and its use, wherein the Kluyveromyces marxianus strain is Kluyveromyces marxianus AMCC30648, deposited with the China Center for the Preservation of Typical Cultures (CCTCC) with deposit number CCTCC NO:M 20222108, or Kluyveromyces marxianus AMCC31343, deposited with the China Center for the Preservation of Typical Cultures (CCTCC) with deposit number CCTCC NO:M 20222111. The Kluyveromyces marxianus strain is characterized by producing a variety of flavor substances at high concentrations. It can be used in fields such as brewing, beverages, and bakeries.

The invention is directed to an integrated CO2 reduction and fermentation method and an apparatus therefor, said method comprising reducing carbon dioxide in a three-compartment electrochemical cell to produce obtain a carbon dioxide reduction product; leading a lean fermentation medium that is lean in the carbon dioxide reduction product into the extraction compartment of the electrochemical cell and leading a rich fermentation medium that is rich in the carbon dioxide reduction product out of the extraction compartment; and leading the rich fermentation medium into a fermentation unit.

This invention discloses a *Bacillus amyloliquefaciens* strain derived from daqu (a type of starter culture) and possessing multiple tolerances and hexanoic acid production characteristics, along with its isolation method and applications. This strain, isolated from daqu, produces hexanoic acid and exhibits resistance to high temperatures, acids, and ethanol; it has been identified as *Bacillus amyloliquefaciens*. B. amyloliquefaciens DJ001, deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 33299, enhances the hexanoic acid production and saccharification power of daqu (a type of starter culture) when added to it, and increases the yield and ethyl hexanoate content of baijiu (Chinese liquor) when added to the mash. This invention has significant value in improving baijiu yield and quality, saving costs, and increasing efficiency, thus increasing benefits for enterprises and society.

In order to control plant diseases caused by Xanthomonas bacteria, a novel bacteriophage exhibiting bacteriolytic activity specific to the same bacteria is isolated, and a plant disease control composition containing the same as an active ingredient is developed and provided. Provided are a lysozyme containing a bacteriophage having a novel genomic DNA sequence and exhibiting a lysolytic activity specific to Xanthomonas bacteria, and a plant disease control composition containing the lysozyme as an active ingredient.

Spun fibers that include fungal biomass are disclosed, as are methods for making and using such spun fibers. In some embodiments, a fungal biomass-based fibrous material is formed by extruding a spun fiber starting mixture comprising the fungal biomass into a desired spatial configuration and subsequently treating the spun fiber starting mixture to form the fibrous material.

The invention discloses a method for increasing the yield of monoterpene glycoside in an endophytic fungus fermentation product separated from paeonia lactiflora, and relates to the technical field of plant fermentation. The method comprises the following steps: material treatment; separating strains; identifying strains; preparing a strain and an elicitor: drying and crushing the overground part of the Chinese herbaceous peony by using the endophytic fungus ER6 separated and identified in the step S3, and sieving with a 80-mesh sieve to prepare powder as a key elicitor; a potato glucose liquid culture medium is used as a basic fermentation culture medium, an activated ER6 strain is inoculated into a fermentation container filled with a sterilized PDB culture medium, and dry powder of the overground part of paeonia lactiflora is added into the culture medium to serve as a biological elicitor to be cultured in a shaking table; and harvesting fermentation liquor. According to the method, a specific endophytic fungus ER6 is separated from paeonia lactiflora roots, and it is found that the yield of monoterpene glycoside in a fermentation product can be remarkably increased by using the overground part of paeonia lactiflora as a biological elicitor to ferment the endophytic fungus ER6.

This invention discloses a strain of *Bifidobacterium pseudosporidis*, its preparation method, and its application in the prevention and treatment of METH addiction. *Bifidobacterium pseudosporidis* was isolated from fecal samples of individuals with low levels of METH addiction, and its strain identity was confirmed after isolation, purification, and identification. A METH addiction model was constructed by gavage colonization of C57 mice with this strain, and addiction-related indicators were detected in the model mice. The results showed that compared with the addiction model mice without colonization of this strain, the conditioned place preference score of the colonized group was significantly reduced (P<0.05). This *Bifidobacterium pseudosporidis* has a significant effect on improving METH addictive behavior and can be used to prepare biological agents with preventive and / or therapeutic functions for METH addiction. This invention reveals that *Bifidobacterium pseudosporidis* has the advantages of high safety and strong targeting, and can achieve the prevention and intervention of METH addiction without complex treatment equipment, providing a novel biological treatment option for the clinical treatment of METH addiction.

The application discloses a heavy metal resistant bacillus velezensis and a microbial repair agent and application thereof. The bacillus velezensis (Bacillus velezensis DBS01) is screened from heavy metal contaminated soil in Dabaoshan mine area of Shaoguan city in Guangdong province, and is preserved in China Center for Type Culture Collection with a preservation number of CCTCC NO: M 20252228. The bacillus velezensis not only has multiple heavy metal resistance capabilities, but also can metabolically secrete organic acids, cellulase, growth hormones and other metabolites in different growth stages, so as to realize multiple functions such as heavy metal solidification, phosphorus dissolution, potassium dissolution and plant growth promotion. The bacillus velezensis DBS01 is inoculated into a culture medium containing straws, calcium superphosphate is added, and agar and sodium alginate are added, and the mixture containing the bacillus velezensis DBS01 is freeze-dried, so that the mine heavy metal contaminated soil can be repaired in a high-efficiency, low-cost and environment-friendly manner, and the ecological function recovery of the soil can be effectively promoted.

The invention discloses a lactobacillus brevis with broad-spectrum bacteriostatic activity and a separation method and application thereof, and belongs to the technical field of microorganisms, in particular to a lactobacillus brevis AW04 with broad-spectrum bacteriostatic activity, which is characterized in that the lactobacillus brevis AW04 is preserved in the China Center for Type Culture Collection, the lactobacillus brevis AW04 is preserved in the China Center for Type Culture Collection, the lactobacillus brevis AW04 is preserved in the China Center for Type Culture Collection, and the lactobacillus brevis AW04 is preserved in the China Center for Type Culture Collection. The address is Wuhan University Campus, Wuchang District, Wuhan City, Hubei Province; the postcode is 430072; the preservation number of the strain is CCTCC M 20241970; the preservation date is September 13, 2024. The strain provides a novel, safe and efficient strain resource for developing microecological preparations, feed additives or veterinary drugs for preventing and treating bacterial diarrhea of animals such as calves and the like, and has important value for reducing the use of antibiotics in the breeding industry and coping with bacterial drug resistance crisis. The strain can be produced on a large scale by using the existing fermentation technology and is convenient to apply.

To provide technologies for easily and efficiently separating cells with different expression levels of undifferentiated markers from cell populations with different expression levels of undifferentiated markers present on the surface of cells.SOLUTION: The above problem is solved by preparing a plurality of adsorbents with different immobilization of fucose-binding proteins on water-insoluble carriers, bringing cell populations with different expression levels of undifferentiated sugar chain markers first into contact with an adsorbent with a low immobilization amount of fucose-binding proteins, then, gradually into contact with an adsorbent with a high immobilization amount of fucose-binding proteins, and separating and collecting cells with different expression levels of undifferentiated sugar chain markers present on the surface of cells.SELECTED DRAWING: Figure 2

The invention relates to a biological agent, in particular to a preparation method of the biological agent, which comprises the following steps: S1, injecting a cultured biological bacterium culture solution into a centrifugal space formed by a plurality of separation rings and a plurality of separation cavities; s2, a plurality of separation rings and a plurality of separation cavities are driven to rotate, and a plurality of water spraying holes spray liquid from top to top to impact the biological bacteria; s3, the biological bacteria with large mass are left on the lower side, and the biological bacteria with small mass are flushed to the upper side by the liquid; s4, after the biological bacteria are separated according to the mass, a plurality of separation rings rotate to discharge the liquid; s5, the biological bacteria are left in the centrifugal space; s6, pressure gas is introduced into a plurality of separation cavities, and an elastic inclined plate is inclined and expands to separate the biological bacteria according to the mass; and the biological bacteria can be centrifugally classified according to different masses.

The application discloses a Campylobacter-specific protein antibody, an antibody-coupled magnetic bead and application. The antibody is a monoclonal antibody 1A10 and a monoclonal antibody 3D9, the monoclonal antibody 1A10 comprises a light chain variable region and a heavy chain variable region, the amino acid sequences of the light chain variable region and the heavy chain variable region of the monoclonal antibody 1A10 are shown in SEQ ID NO: 7 and 8 respectively; the monoclonal antibody 3D9 comprises a light chain variable region and a heavy chain variable region, the amino acid sequences of the light chain variable region and the heavy chain variable region of the monoclonal antibody 3D9 are shown in SEQ ID NO: 9 and 10 respectively. The antibody-coupled magnetic bead is coupled by the monoclonal antibody 1A10, the monoclonal antibody 3D9 and activated carboxyl magnetic beads respectively. The antibody-coupled magnetic bead can specifically enrich Campylobacter, has high enrichment efficiency, simple enrichment operation, can be applied to metabolic product analysis of Campylobacter, and can be used for qualitative and quantitative analysis of the Campylobacter.

The invention discloses a proteophilic bacterium strain and application thereof, and relates to the technical field of wine brewing microorganisms. The proteiniphilum sp. Strain K 5-3-1 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on June 05, 2023, and the preservation number of the proteiniphilum sp. Strain K 5-3-1 is CGMCC NO.27523. The proteiniphilum sp. Strain K 5-3-1 has the advantages that the preservation number is CGMCC NO.27523; the new strain provided by the invention is separated from pit mud of a strong-flavor liquor pit, is high in acetic acid yield and can be applied to the field of liquor brewing.

This invention provides the preparation and application of a strain of *Lactiplantibacillus plantarum* INM3104 that reduces raffinose in whole grain dough. This strain, *Lactiplantibacillus plantarum* INM3104, which degrades raffinose, was deposited at the Guangdong Provincial Microbial Culture Collection Center on April 11, 2024, with accession number GDMCC NO:64512. The advantages are: it only reduces the raffinose content in whole grain products, while also improving the flavor and quality of the whole grain products; the whole grain products produced using this preparation do not affect the overall processing of the whole grain products after fermentation, not only improving the taste of the whole grain products but also reducing the adverse factors associated with whole grain products.

The present application belongs to a kind of microalgae sedimentation technology, more particularly to a kind of microalgae sedimentation agent and its application.The present application specifically discloses a kind of sedimentation agent and its composition, the sedimentation agent contains planarian body and / or planarian extract, the sedimentation agent is co-cultured with the microalgae cell of appropriate density, so that microalgae is naturally settled;The planarian extract is obtained by filtering the solution of planarian cultivation.The sedimentation agent and its composition of the present application are natural sedimentation agents for dunaliella salina, and do not introduce impurities and toxic substances;Therefore, the amount of pheromone used in the present application is very small, the efficiency of coagulation is high, the chemical interference is small, and as a natural sedimentation agent, there is no toxicity.

The present disclosure relates to filters configured for a spectroscopic antibiotic susceptibility analysis and a method for filtering a sample for a spectroscopic antibiotic susceptibility analysis. In one example, a method comprises providing a solution comprising bacteria incubated in a media to a well, filtering the solution via adsorption to trap at least a portion of the bacteria in a filter separate from the media. providing a solution of SERS active nanoparticles to the well, and spectroscopically analyzing the filter to determine the presence and / or absence of at least one of a purine and a pyrimidine. Methods of providing an internal standard in a spectroscopic antibiotic susceptibility test are also provided.

The invention discloses chlorella capable of resisting acetate and urea and a breeding method and application thereof, and relates to the technical field of microorganisms, the chlorella is a Sorokiniana mutant strain, the classification name of the chlorella is Chlorella sorokiniana-TongWei-01, the chlorella is preserved in the China General Microbiological Culture Collection Center at 2025-12-1, and the preservation number of the chlorella is CGMCC NO. The address of the preservation unit is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.46630. According to the method, large-scale culture can be performed by taking acetic acid / sodium acetate as a unique carbon source and urea as a unique nitrogen source, the method has excellent acetic acid utilization efficiency and urea tolerance, toxicity interference is resisted while two substrates are efficiently converted, high-density culture supported by a cheap carbon-nitrogen source is realized, and a core algae species support is provided for large-scale production of microalgae.

The embodiment of the invention discloses yarrowia caribeckii as well as a separation and screening method, an identification method and application of the yarrowia caribeckii. The preservation number of the yarrowia caribica is CGMCC (China General Microbiological Culture Collection Center) No.27604, and the preservation number of the yarrowia caribica is CGMCC No.27604. The Caribik Yarrowia spp. Provided by the embodiment of the invention has a good antagonistic effect on gray mold, penicilliosis, black spot and aspergillus niger after fruits are picked, and can obviously reduce the disease morbidity of the fruits of strawberries, apples, tomatoes and pears. And the antagonistic bacterium is identified to be Meyerozyma caribbica, and the antagonistic bacterium can be used as the Meyerozyma caribbica. By respectively adding auxiliary factors, namely calcium chloride, alginate oligosaccharide (AOS), phytic acid (PA) and metschhia pastoris, the disease prevention and control effects of Meyerozyma caribbica can be enhanced. The disease prevention and control effects of the Meyerozyma caribbica can be enhanced by adding the auxiliary factors of calcium chloride, alginate oligosaccharide (AOS), phytic acid (PA) and metschhia pastoris.

The application belongs to the technical field of tertiary oil recovery, and particularly relates to a method for increasing production by microbial composite huff and puff and application thereof. The method for increasing production by microbial composite huff and puff comprises the following steps: (1) reservoir screening; (2) functional microbial system screening; and (3) physical model evaluation method. The application realizes the effects of the system and injection parameters by innovative indoor evaluation method, physical model displacement pressure and enhanced oil recovery. The application finally realizes the purposes of improving the channel blockage problem caused by high-wax crude oil and efficient development of high-wax crude oil in the reservoir, and the average single-well daily production is increased by more than 2t, and the economic efficiency is greater than 1:5.

This invention relates to a method for rapidly screening filamentous fungi that produce high levels of triglycerides and polyunsaturated fatty acids, belonging to the field of microbial technology. The method employs a two-stage screening strategy of "resistance primary screening – fluorescence secondary screening." In the primary screening stage, strains with strong lipid synthesis capabilities are initially screened by adding a combination of phytocyanin and quizalofop-p-ethyl inhibitors to the culture medium. In the secondary screening stage, cellophane culture technology and Nile red fluorescence staining are combined to quantitatively assess the triglyceride content of the colonies, achieving rapid and non-destructive screening. This method is simple to operate, highly efficient, and shows a significant positive correlation between fluorescence intensity and triglyceride content. It is suitable for mutagenesis breeding and industrial strain selection of oil-producing filamentous fungi such as *Morchella alpineensis*.

Chromatographic media comprising porous beads having an inner porous core and an outer porous shell are used for the chromatographic separation of enveloped or membrane-bearing biological particles from impurities such as contaminating DNA and / or proteins. The core is capable of binding molecules through hydrophobic interactions; however, the pore size of the shell prevents particles with a size of 20 nm or larger from penetrating into the beads and interacting with the core. Separation is performed at a pH below 7.4. The enveloped or membrane-bearing biological particles may have been subjected to a prior chromatographic capture step. When used for the purification of enveloped viral particles, the method of the present invention has been found to result in a significantly higher rate of infectious viral particles.

The invention belongs to the field of analysis and testing, and particularly relates to WGA-ConA double lectin modified magnetic nanoparticles for broad-spectrum separation of pathogenic bacteria as well as a preparation method and application of the WGA-ConA double lectin modified magnetic nanoparticles. The invention provides a WGA-ConA double lectin modified magnetic nanoparticle for broad-spectrum separation of pathogenic bacteria. The capture efficiency of the WGA-ConA double lectin modified magnetic nanoparticle on staphylococcus aureus, listeria monocytogenes, escherichia coli, pseudomonas aeruginosa and the like is greater than or equal to 85% under the condition that the pH is 6.5-7.5.