785results about "Microorganism separation" patented technology

The invention belongs to the technical field of water treatment, and particularly provides a fungus filament bamboo-based biomass composite material and a lead-containing sewage treatment method. According to the invention, the lead-containing sewage is pretreated by using microorganism neurospora crassa, and then the lead-containing sewage is post-treated by using the fungus filament bamboo-based biomass composite material prepared under specific conditions, so that the mildness of microorganism treatment and the efficient adsorbability of the biomass composite material are effectively combined; and finally, the removal rate of heavy metal lead is increased, the cost is low, and environmental friendliness is achieved.

The invention provides a method for repairing and maintaining an ecological bank protection structure of a water conservancy project. Comprising the following steps: S1, screening and domesticating functional microorganisms; S2, constructing a vegetation and microorganism synergistic remediation unit; S3, precisely arranging the synergistic remediation unit on site; S4, dynamically monitoring and coordinately regulating and controlling: screening and domesticating the functional microorganisms, constructing the synergistic remediation unit, precisely arranging the synergistic remediation unit on site, and dynamically monitoring and regulating and controlling. A microorganism-vegetation-carrier synergistic effect mechanism is formed, so that the technical problems of structural stability and ecological function separation in traditional revetment restoration are solved; the method has the advantages that the synergistic interaction of structure stability and ecological restoration is realized, the self-repairing capability of a microorganism-vegetation symbiotic system is improved, a multi-dimensional dynamic monitoring regulation and control mechanism is established, and the manual maintenance cost is reduced.

The present invention provides a method for producing extracellular vesicles, the method including a step for passing a crushed liquid of plants, mushrooms, or microalgae through a depth filter, and a step for subjecting the crushed liquid after passing through the depth filter to ultrafiltration treatment, the depth filter being composed of filaments.

The invention discloses a lactobacillus rhamnosus strain with antiallergic activity and a preparation method thereof, the strain is lactobacillus rhamnosus Lrn-PG-06, the classification name of the strain is Lactobacillus rhamnosus, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.3077, the preservation time is March 21, 2024, the preservation unit is China General Microbiological Culture Collection Center, the preservation number is CGMCC No.3077, and the preservation number is CGMCC No.3077. The address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The lactobacillus rhamnosus Lrn-PG-06 strain is high in acid resistance, alkali resistance and cholate resistance, high in simulated gastrointestinal fluid resistance and high in oxidation resistance, has the advantage of resisting allergy, and solves the problems that at present, allergic diseases are mainly treated by drugs or are prevented from being in contact with allergens in the environment and food, but due to the fact that the drugs are large in toxic and side effects, the allergens cannot be in contact with the allergens in the environment and food. And allergens causing anaphylaxis are difficult to determine, so that allergic diseases are difficult to treat or prevent.

The invention discloses a bacillus subtilis strain and application thereof in prevention and treatment of tomato brown wrinkled fruit virus diseases. According to the bacillus subtilis strain, the bacillus subtilis strain is NA25, the preservation name is bacillus subtilis, the bacillus subtilis strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), the address of the preservation unit is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation date is April 27, 2025; the preservation number of the strain is CGMCC No. 34380. The bacillus subtilis provided by the invention is separated from healthy tomato endophyte, and the strain fermentation liquor and cell suspension both have a good inhibition effect on the brown wrinkled fruit virus, and can be used for preventing and treating the tomato brown wrinkled fruit virus.

The embodiment of the invention discloses a Yarrowia guilliermondii as well as a separation and screening method, an identification method and application of the Yarrowia guilliermondii. The preservation number of the Yarrowia guilliermondii is CGMCC (China General Microbiological Culture Collection Center) No.27603, and the preservation number of the Yarrowia guilliermondii is CGMCC No.27603. The Yarrowia guilliermondii disclosed by the embodiment of the invention has a very good prevention and control effect on postharvest diseases of fruits, and the prevention and control effect of the Yarrowia guilliermondii on the diseases can be enhanced by respectively adding auxiliary factors, namely calcium chloride, alginate oligosaccharide (AOS), phytic acid (PA) and metschhia yeast. Through identification, the antagonistic bacterium is a Yeyrozyma guilliermondii, and the antagonistic bacterium is a Yeyrozyma guilliermondii. The M.guilliermonii has a good control effect on gray mold, penicilliosis, black spot and aspergillus niger of strawberry, apple, pear and tomato fruits, can be rapidly colonized on fruit wounds, and can enhance the resistance-related enzyme activity of the fruits, occupy the wound space and induce the fruit resistance to reduce the morbidity of diseases.

The invention belongs to the field of membrane pollution control, and particularly relates to pseudomonas as well as a separation method and application thereof. The invention provides a pseudomonas, which is identified as Pseudomonas knackmussii HITSZ-Q1, is preserved in the Guangdong Microbiological Culture Collection Center, and has the preservation number of GDMCC (China General Microbiological Culture Collection Center) NO: 66162. The strain takes C6-HSL as the unique carbon source and nitrogen source, and the 16S rDNA sequence of the strain is as shown in SEQ ID NO. 1. The strain has a remarkable degradation capability on signal molecules such as C6-HSL, C8-HSL, 3-oxo-C12-HSL and the like, and the highest quenching rate can reach 98.9%. The strain and pseudomonas aeruginosa are co-cultured, so that the signal molecule concentration of the pseudomonas aeruginosa can be effectively reduced, and biofilm formation is inhibited. The strain is further prepared into a carrier in the form of immobilized pellets, the carrier is added into a membrane bioreactor, the operation cycle is prolonged from 6.5 days to 10.5 days, and the membrane pollution delay rate reaches up to 61.5%.

The invention discloses heavy metal resistant bacillus velezensis as well as a microbial remediation agent and application thereof. Bacillus velezensis DBS01 is screened from heavy metal contaminated soil in Dabaoshan mining areas in Shaoguan City, Guangdong province, is preserved in China Center for Type Culture Collection (CCTCC), has a preservation number of CCTCC M 20252228, not only has resistance to various heavy metals, but also can metabolize and secrete metabolites such as organic acid, cellulase and growth hormone in different growth stages, and can be used for preventing and treating various heavy metals. Multiple functions of curing heavy metals, dissolving phosphorus and potassium, promoting plant growth and the like are realized. According to the method, bacillus velezensis DBS01 is inoculated into a culture medium containing straw, calcium superphosphate, agar and sodium alginate are added, and a mixture containing the bacillus velezensis DBS01 is freeze-dried, so that the heavy metal contaminated soil in the mining area can be efficiently repaired in an environment-friendly manner at low cost, and the ecological function recovery of the soil is effectively promoted.

The invention belongs to the field of microorganisms, and particularly relates to bacillus Z161 capable of efficiently degrading straw as well as a screening method and application of the bacillus Z161. The bacillus subtilis Z161 is bacillus mexicanus, the preservation number of the bacillus mexicanus Z161 is CGMCC (China General Microbiological Culture Collection Center) NO.36393, and the bacillus mexicanus Z161 is preserved in the China General Microbiological Culture Collection Center on October 29, 2025. The bacillus Z161 screened by the invention has strong laccase activity and high temperature resistance, and can efficiently degrade lignin under the condition of 50 DEG C; the problem that in the prior art, normal-temperature zymophyte is mostly adopted, and the requirement for fermentation conditions in the fermentation process is harsh is solved, and therefore the rapid compost fermentation technology is easier to achieve. The bacillus Z161 provided by the invention has the advantages of genetic and functional character stability, has good tolerance at high temperature, enables lignin to be quickly depolymerized, and improves the decomposition efficiency.

The invention relates to the field of microorganisms, in particular to lactobacillus plantarum TRT-01 with anti-inflammatory and bacteriostatic functions as well as a preparation method and application of the lactobacillus plantarum TRT-01, the lactobacillus plantarum TRT-01 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.32237, and the preservation date is October 17, 2024. The plant lactobacillus has broad-spectrum bacteriostatic activity on 10 pathogenic bacteria such as helicobacter pylori, streptococcus mutans and the like, and can regulate Th1 / Treg immune balance and relieve inflammatory response by inducing dendritic cells to secrete cell factors IL-10 and IL-12, inducing T cells to secrete cell factors TGF-beta and IFN-gamma and inhibiting secretion of cell factors IL-6 at the same time. Therefore, the plant lactobacillus TRT-01 disclosed by the invention can be used for preparing a composition with anti-inflammatory and bacteriostatic effects, is suitable for the fields of medicines, foods, health-care products and the like, and is particularly suitable for developing a microbial preparation with a probiotic effect.

The invention belongs to the technical field of probiotics, and particularly relates to probiotics for improving intestinal health and application thereof. Wherein the probiotics for improving the intestinal health are a Lactobacillus plantarum LV1000 strain with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.30865, and the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on June 5, 2024; through unique activation culture, streak separation and bacterial colony identification steps, high activity and high purity of the strain are ensured; based on the application of the strain, two product forms of probiotic microcapsules and probiotic compositions are developed, the probiotic microcapsules adopt sodium alginate, calcium lactate and other raw materials, the intestinal colonization ability of the strain is effectively improved, and the synergistic interaction of oxidation resistance and immunoregulation is achieved by combining rosa roxburghii tratt freeze-dried powder; and the probiotic composition can comprehensively regulate the balance of intestinal flora and improve the digestive function and intestinal motility.

This invention discloses a *Propionibacterium japonicum* strain selected through space-based combined chemical mutagenesis and its mutagenesis, screening, and propionic acid fermentation methods. Using *Propionibacterium japonicum* strain CICC22741 from the China Industrial Microbiological Culture Collection Center as the starting strain, this invention employs space-based mutagenesis via the "Shijian-19" satellite combined with ethyl methanesulfonate chemical mutagenesis. After two rounds of mutagenesis screening, a high-propionic acid-producing strain, *Propionibacterium japonicum* DJ04-16, was obtained. This strain was deposited at the China General Microbiological Culture Collection Center on June 16, 2025, with accession number CGMCC No. 34914. Subsequently, the propionic acid fermentation conditions of *Propionibacterium japonicum* DJ04-16 were optimized. Validation was performed in a 1-ton fermentation tank. After 96 hours of fermentation, the highest propionic acid yield reached 67.36 g / L, with a fermentation intensity of 0.702 g / L / h. This strain exhibits good industrial fermentation potential.

The invention discloses a method for leaching waste lithium battery positive electrode powder by using intestinal flora in a neutral environment, and belongs to the technical field of resource recycling, the method comprises the following steps: step 1, a pretreatment link, the pretreatment link comprises the following process links, and each link has no sequence: (1) preparation of battery powder, (2) acquisition of intestinal flora, and (3) preparation of a culture medium; 2, domesticating the flora; and 3, valuable metal in the waste battery powder is leached out. According to the method, the intestinal flora is applied to the biological leaching of the waste LIBs positive electrode powder, so that the dependence of traditional strong acidophilic bacteria on an extremely low pH condition is broken through, and the environmental harm is greatly reduced; according to the method, inorganic acid does not need to be added, the pH value of a reaction system is stabilized within the nearly neutral range of 7.8-8.2, equipment loss caused by acid etching is reduced, reagent consumption for subsequent metal recovery and neutralization treatment is reduced, and remarkable economic benefits and environmental benefits are achieved.

The invention discloses a novel bifidobacterium infantis strain and application of extracellular products of the novel bifidobacterium infantis strain in delaying skin aging and regulating skin micro-ecology, and bifidobacterium infantis is bifidobacterium infantis BSJM24L007 which is preserved in the China General Microbiological Culture Collection Center (CGMCC for short) and has the preservation number of CGMCC No.33258. The invention further discloses a preparation method of the novel bifidobacterium infantis strain and the application of the extracellular products of the novel bifidobacterium infantis strain. The bifidobacterium infantis extracellular product prepared by the invention has an ideal effect of delaying skin aging, enables the skin to be more compact and elastic, reduces the generation of wrinkles and maintains normal metabolism of the skin, has relatively high safety, can effectively reduce the skin infection problem caused by staphylococcus aureus and propionibacterium acnes, and has a good application prospect. Skin infection and inflammation are reduced, skin health is promoted, and skin micro-ecological balance is maintained. The requirements of a modern skin external preparation on functionality and safety are met.

The invention discloses Wickerhamomyces anomalus for degrading patulin and an application of the Wickerhamomyces anomalus. The Wickerhamomyces anomalus strain XL1 obtained through screening shows excellent degradation performance of the patulin, and the highest degradation rate can reach 100% in an artificial culture medium and an actual application scene. Taking apple juice treatment as an example, the strain can quickly and thoroughly remove patulin pollution therein, and the food safety quality is remarkably improved. In addition, the strain XL1 is separated from white spirit grains, is natural and safe in source, has no biological safety risk when being applied to food systems such as fruit juice and the like, does not cause environmental pollution, and has the advantages of being green and environment-friendly. The method has the advantages of high efficiency, safety and environmental friendliness, and has wide application prospect and industrialization value in the fields of food processing and the like.

The present disclosure pertains to an extracellular vesicle purification kit that contains a component that does not dissolve in pure water but dissolves in a chelating agent aqueous solution.

The invention relates to a gastric acid-resistant sustained-release intestinal bacteria capsule based on bionic mucous membrane-enzymolysis response and a preparation method thereof. The gastric acid-resistant sustained-release intestinal bacteria capsule comprises a core layer, an adhesive layer, an outer-layer composite film and a protective coating from inside to outside, the core layer consists of intestinal flora and a multifunctional freeze-drying protective agent; the adhesive layer is composed of trehalose, N-acetyl glucosamine and MUC2 recombinant protein; the outer-layer composite film is composed of a base material and a functional filler; the protective coating is composed of beewax and chitosan. The anti-gastric acid sustained-release intestinal bacteria capsule based on bionic mucosa-enzymolysis response provided by the invention has excellent gastric acid resistance, and the gastric acid resistance is remarkably improved; good slow release performance is achieved, and accurate slow release of enzymatic hydrolysis response can be achieved; the colonization efficiency of the intestinal bacteria can be effectively improved; the storage stability is good. The preparation method provided by the invention has the advantages of simplified steps, short time consumption, controllable core operation time and obviously shortened whole process, and is beneficial to keeping the activity of the flora.

The invention discloses a method for rapidly enriching pit mud high-yield caproic acid functional flora and application, and belongs to the technical field of baijiu brewing microorganisms. According to the method, the aged pit mud which is screened through tests and has better quality is used as seed mud, and the selected high-quality pit mud is subjected to step-by-step amplification culture by adopting a specific culture medium. The obtained bacterial community is a caproic acid producing bacterial community (85%) with the caproic acid producing clostridium as the main body, and the bacterial community can produce more than 10 g / L of caproic acid and less than 2 g / L of butyric acid. The mixed flora obtained by the method disclosed by the invention can be used for culturing and repairing the pit mud, increasing the caproic acid-producing main flora in the pit mud, promoting the pit mud to be aged, preventing the pit mud from being aged and hardened, and effectively improving the quality of raw wine at the same time. And the mixed flora can also be used for fermenting the byproduct yellow water of the Luzhou-flavor liquor to produce caproic acid at high yield, and can be further used for producing high-quality flavoring liquor.

The invention discloses a method for improving the flavor of mulberry wine by screening aroma-producing yeast from mulberries. The method relates to the technical field of microorganisms and solves the problem that the existing mulberry wine preparation process cannot fully reflect the nutritional value and unique flavor of mulberries. Low-temperature treatment is used to increase the relative abundance of target bacterial genera in a sample, and a sample with a high relative abundance of desired bacterial species and a low fungal count is selected. Then, a specific screening method is adopted to separate and molecularly identify the aroma-producing yeast. Yeast with good tolerance and the ability to produce a strong aroma is selected from the sample through tolerance experiments and electronic nose analysis, and the yeast is compounded into a starter. The alcohol content of the obtained mulberry wine is increased by more than 2.2% vol to 13.5% vol, the total ester is increased by 25%, and the relative content of aromatic compounds such as aldehydes and ketones is increased. The aroma-producing yeast species are obtained through directional separation, which can avoid blindness, improve screening efficiency, and enhance the flavor of the fruit wine.

The invention relates to selenium-enriched pediococcus acidilactici Se34 with anti-oxidation, cholesterol-lowering and anti-tumor effects and application of the selenium-enriched pediococcus acidilactici Se34. The pediococcus acidilactici Se34 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number of the pediococcus acidilactici Se34 is CGMCC No.33933, and the preservation date of the pediococcus acidilactici Se34 is March 24, 2025. The pediococcus acidilactici Se34 provided by the invention integrates excellent probiotic characteristics (acid-resistant bile salt, safe and colonizable), efficient selenium enrichment capacity and remarkably enhanced activity of resisting oxidation, reducing cholesterol and inhibiting specific cancer cell proliferation and metastasis after selenium enrichment. The strain and the selenium-rich product thereof have important application value in the fields of developing functional foods (selenium-rich probiotic products), antioxidant / cholesterol-reducing health-care foods, potential auxiliary anticancer biological preparations or prodrugs and the like.

A cell picking device for sucking cells from a liquid sample in a sample container includes a sucking member to which a pipette tip is attachable, a driver that moves the sucking member and performs suction through the sucking member and the pipette tip, a work mode switcher that switches a work mode of the driver between a first mode and a second mode, and a controller that controls the driver.

The invention discloses a preparation method of engineered bacteria for releasing CO gas under initiation of ultrasonic waves to kill tumor cells, and relates to a preparation method of CO carrier engineered bacteria. The invention aims to solve the problems that the existing CO carrier material is low in delivery efficiency, the CO is difficult to release efficiently and controllably in space and time at the same time, and the existing metal CO carrier material is high in toxicity. Meanwhile, the problems that the penetrating power of light waves to biological tissues is limited, and the CO release efficiency is limited due to a tumor hypoxic microenvironment are solved. The preparation method comprises the following steps: 1, modifying the surface of escherichia coli with 3-hydroxyflavone-D-alanine; and 2, continuously modifying the surface of the escherichia coli with CaO2 (at) polymyxin B. The method is used for preparing the engineered bacteria for releasing the CO gas through ultrasonic initiation to kill the tumor cells.

The present disclosure provides a method for processing extracellular vesicles in which the extracellular vesicles are not purified prior to contacting with a fluorescent staining dye or antibody. By utilizing a centrifugal filter, excess staining dye or antibody can be easily removed prior to analysis of one or more characteristics of the extracellular vesicles. The method provides for rapid and simple processing and analysis while maintaining a high concentration of extracellular vesicles.

This invention relates to the Kluyveromyces marxianus strain and its use, wherein the Kluyveromyces marxianus strain is Kluyveromyces marxianus AMCC30648, deposited with the China Center for the Preservation of Typical Cultures (CCTCC) with deposit number CCTCC NO:M 20222108, or Kluyveromyces marxianus AMCC31343, deposited with the China Center for the Preservation of Typical Cultures (CCTCC) with deposit number CCTCC NO:M 20222111. The Kluyveromyces marxianus strain is characterized by producing a variety of flavor substances at high concentrations. It can be used in fields such as brewing, beverages, and bakeries.

The invention is directed to an integrated CO2 reduction and fermentation method and an apparatus therefor, said method comprising reducing carbon dioxide in a three-compartment electrochemical cell to produce obtain a carbon dioxide reduction product; leading a lean fermentation medium that is lean in the carbon dioxide reduction product into the extraction compartment of the electrochemical cell and leading a rich fermentation medium that is rich in the carbon dioxide reduction product out of the extraction compartment; and leading the rich fermentation medium into a fermentation unit.

This invention discloses a *Bacillus amyloliquefaciens* strain derived from daqu (a type of starter culture) and possessing multiple tolerances and hexanoic acid production characteristics, along with its isolation method and applications. This strain, isolated from daqu, produces hexanoic acid and exhibits resistance to high temperatures, acids, and ethanol; it has been identified as *Bacillus amyloliquefaciens*. B. amyloliquefaciens DJ001, deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 33299, enhances the hexanoic acid production and saccharification power of daqu (a type of starter culture) when added to it, and increases the yield and ethyl hexanoate content of baijiu (Chinese liquor) when added to the mash. This invention has significant value in improving baijiu yield and quality, saving costs, and increasing efficiency, thus increasing benefits for enterprises and society.

In order to control plant diseases caused by Xanthomonas bacteria, a novel bacteriophage exhibiting bacteriolytic activity specific to the same bacteria is isolated, and a plant disease control composition containing the same as an active ingredient is developed and provided. Provided are a lysozyme containing a bacteriophage having a novel genomic DNA sequence and exhibiting a lysolytic activity specific to Xanthomonas bacteria, and a plant disease control composition containing the lysozyme as an active ingredient.

Spun fibers that include fungal biomass are disclosed, as are methods for making and using such spun fibers. In some embodiments, a fungal biomass-based fibrous material is formed by extruding a spun fiber starting mixture comprising the fungal biomass into a desired spatial configuration and subsequently treating the spun fiber starting mixture to form the fibrous material.

To purify and concentrate differentiated cells derived from an ES cell or an iPS cell or the like without damaging the cells.SOLUTION: An apparatus for analyzing and separating fine particles includes a flow channel cartridge, light irradiation means, detection means for detecting a target fine particle, and force generating means. In the cartridge, a sample reservoir connected to a first flow channel, a fourth branch flow channel and a fifth branch flow channel connected to the first flow channel, a third A reservoir connected to the fourth branch flow channel, a third B reservoir connected to the fifth branch flow channel, and a fourth reservoir reserving unfractionated fine particles are formed, each of the reservoirs has means for making pneumatic pressure inside each reservoir and pneumatic pressure of a pneumatic pressure control system in each apparatus become the same, and the apparatus controls a flow of flow channels inside the cartridge by controlling pneumatic pressure inside each reservoir by each pneumatic pressure control system in the apparatus.SELECTED DRAWING: Figure 3

The invention discloses a method for increasing the yield of monoterpene glycoside in an endophytic fungus fermentation product separated from paeonia lactiflora, and relates to the technical field of plant fermentation. The method comprises the following steps: material treatment; separating strains; identifying strains; preparing a strain and an elicitor: drying and crushing the overground part of the Chinese herbaceous peony by using the endophytic fungus ER6 separated and identified in the step S3, and sieving with a 80-mesh sieve to prepare powder as a key elicitor; a potato glucose liquid culture medium is used as a basic fermentation culture medium, an activated ER6 strain is inoculated into a fermentation container filled with a sterilized PDB culture medium, and dry powder of the overground part of paeonia lactiflora is added into the culture medium to serve as a biological elicitor to be cultured in a shaking table; and harvesting fermentation liquor. According to the method, a specific endophytic fungus ER6 is separated from paeonia lactiflora roots, and it is found that the yield of monoterpene glycoside in a fermentation product can be remarkably increased by using the overground part of paeonia lactiflora as a biological elicitor to ferment the endophytic fungus ER6.