1086results about "Microorganism separation" patented technology

The invention discloses a method for denitrifying wastewater by using a low-temperature-resistant heterotrophic nitrification and aerobic denitrification bacterium, and relates to a method for denitrifying wastewater by using a biological bacterium, the bacterium is Pseudomonas putida, is named as Pseudomonas umsongensis BK17, belongs to Pseudomonas, and is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC M 20242924, and the preservation date is December 27, 2024. The strain is a heterotrophic nitrification-aerobic denitrification bacterium, can efficiently remove inorganic nitrogen pollution in a water body, still has good denitrification performance under low-temperature stress, and is high in low-temperature tolerance. The strain can efficiently treat nitrogen-containing wastewater under the condition of 10 DEG C, and the removal efficiency can reach 99% or above. The method has great potential in the application of inorganic nitrogen pollution treatment of domestic sewage and other water bodies in cold regions in China.

The invention belongs to the technical field of water treatment, and particularly provides a fungus filament bamboo-based biomass composite material and a lead-containing sewage treatment method. According to the invention, the lead-containing sewage is pretreated by using microorganism neurospora crassa, and then the lead-containing sewage is post-treated by using the fungus filament bamboo-based biomass composite material prepared under specific conditions, so that the mildness of microorganism treatment and the efficient adsorbability of the biomass composite material are effectively combined; and finally, the removal rate of heavy metal lead is increased, the cost is low, and environmental friendliness is achieved.

The invention provides a rice endophyte for promoting rice growth and reducing cadmium as well as a preparation method and application of the rice endophyte, and relates to the field of environmental microbiology and agricultural microorganisms. The rice endophyte is stenotrophomonas, is named as Stentrophomonas sp.R5, is preserved in the China Center for Type Culture Collection, the address is Wuhan University, Wuhan, China, the postcode is 430072, the preservation number is CCTCC NO: M 20231758, and the preservation date is September 20, 2023. The rice endophyte not only has the capabilities of producing IAA, dissolving phosphorus, producing siderophores and the like for promoting the growth and development of rice, but also has an efficient inhibition effect on absorption and transportation of cadmium in the rice, particularly, the cadmium reduction amplitude of mature rice grains reaches up to 57.69%, and the rice endophyte has little influence on microbial communities in a soil environment, and has a good application prospect. Excessive interference is not caused, and conversion of available nutrients in the soil is promoted.

The invention relates to the technical field of biomineralization, and particularly discloses an alkali-resistant urease-producing microbial flora for biomineralization of construction joints and a preparation method of the alkali-resistant urease-producing microbial flora. The microbial flora disclosed by the invention is prepared from the following strains with relative abundance: 4.4 to 5.2 percent of bacillus, 0.39 to 0.46 percent of enterococcus, 4.8 to 5.7 percent of alcaligenes, 0.50 to 0.58 percent of cellulomonas, 22.4 to 24.2 percent of lysobacter, 1.7 to 2.4 percent of pseudomonas, 10.8 to 11.9 percent of Savagea, 15.1 to 16.2 percent of sarcina, 30.8 to 32.2 percent of chloridomonas and 0.41 to 0.48 percent of bacillus. The alkali-resistant microbial compound flora with high urease activity in the limited oxygen and normal environment can effectively overcome the defects that urease microorganisms are low in mineralization efficiency under the anoxic condition, and the strength of an obtained consolidated body is not high.

The invention discloses propionibacterium capable of obtaining high-yield propionic acid through compound mutation screening and a method for fermenting propionic acid by using the propionibacterium. The high-yield propionic acid strain YCBS010 (CGMCC No.31160) is obtained by carrying out compound mutagenesis on acid-producing propionibacterium CGMCC 1.2232 serving as an original strain through microwave-ultrasonic combined mutagenesis and compound normal-pressure and room-temperature plasma (ARTP) mutagenesis means. According to the invention, YPA15 is used as a starting strain, microwave and ultrasonic combined mutagenesis is carried out for 100 seconds to screen a bacterial strain with increased propionic acid yield, then ARTP mutagenesis is carried out for 90 seconds to screen out a high-yield mutant strain YCBS010, fermentation in a 1-ton tank verifies that the highest propionic acid yield reaches 65.87 g / L and the fermentation intensity is 0.73 g / L.h after fermentation is carried out for 90 hours, and the bacterial strain has better industrial fermentation potential.

The invention provides a plant lactobacillus YH001 with bacteriostatic activity as well as a screening method and application of the plant lactobacillus YH001. The phytobacterium plantarum YH001 has the taxonomic name of Lactiplantibacillus plantarum, is preserved in the Guangdong Microbial Culture Collection Center, and has the preservation number of GDMCC No: 65693, the preservation number of Lactiplantibacillus plantarum YH001 is CGMCC NO: 65693, and the preservation number is CGMCC NO: 65693. The preservation date is December 30, 2024. The strain has excellent biological characteristics of no hemolysis, acid resistance, cholate resistance and the like, and has wide application potential in the fields of food, medicine and the like. The fermentation supernatant still keeps high bacteriostatic activity after being treated at a high temperature of 121 DEG C, and shows excellent thermal stability. Therefore, the strain has a wide application prospect in research and development of antibacterial agents, food leavening agents and preservative products.

The invention relates to the technical field of microorganisms, in particular to bacillus velezensis LPF421 and application thereof. The bacillus velezensis LPF421 is classified and named as bacillus velezensis LPF421, and is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCCNO: 65977. The bacillus velezensis LPF421 is named as bacillus velezensis LPF421 and is preserved in the Guangdong Microbial Culture Collection Center. The bacillus velezensis LPF421 disclosed by the invention can generate amylase and cellulase, and has an antagonistic effect on a plurality of moulds. In addition, a soybean meal fermentation test further proves that the strain not only can obviously reduce the content of soybean antigen protein, but also can increase the content of acid soluble protein.

The invention discloses alkali-producing cadmium-tolerant acinetobacter capable of increasing the pH value of soil and application of the alkali-producing cadmium-tolerant acinetobacter, and belongs to the technical field of microorganisms, the alkali-producing cadmium-tolerant acinetobacter is preserved in the China General Microbiological Culture Collection Center (CGMCC), the number of the alkali-producing cadmium-tolerant acinetobacter is CGMCC No.33619, and the alkali-producing cadmium-tolerant acinetobacter belongs to proteobacteria and pseudomonas. The strain can increase the environmental pH value from 6.0 to 8.0 through metabolism, and stably grows in an environment with the cadmium (Cd < 2 + >) concentration as high as 20 ppm. The strain can be applied to the fields of acid soil improvement, cadmium pollution remediation, industrial wastewater treatment, fermentation engineering and the like. The method has the advantages of being environmentally friendly, efficient, stable, applicable to multiple scenes and the like, and a novel microbial resource is provided for heavy metal pollution treatment and soil ecological restoration.

According to the microbial flora culture method for efficiently degrading the organic pollutants, three continuous culture stages of enrichment, domestication and optimization are adopted, parameters such as the temperature, the pH value, the rotating speed and the ventilatory capacity are accurately controlled in each stage, and growth of microorganisms with degradation capacity is promoted. Through multi-stage culture and domestication, the obtained flora has efficient degradation capability on organic pollutants such as petroleum hydrocarbons, pesticides and polycyclic aromatic hydrocarbons, and has strong adaptability to pollutants with different concentrations. The 16S rRNA gene is used for sequencing and identifying the flora, and composition and dominant strains are clear. The flora is prepared into a microbial inoculum, and the microbial inoculum is preserved by freeze drying or liquid nitrogen, so that the activity can be effectively maintained. The flora can be widely applied to the fields of polluted soil and water body remediation, industrial wastewater treatment and the like, is green and environment-friendly, is relatively low in cost, avoids secondary pollution, conforms to the concept of sustainable development, and has relatively high economic and environmental values.

The invention provides a microecological preparation with an immunological enhancement function and application of the microecological preparation in antibiotic-free breeding of livestock and poultry, and relates to the technical field of agricultural biology. The microecological preparation with the immunological enhancement function comprises a probiotic component, a prebiotics component and an immunological enhancement component, and the probiotic component, the prebiotics component and the immunological enhancement component are respectively composed of lactic acid bacteria, bacillus, clostridium butyricum, fructo-oligosaccharide, yeast beta-glucan, astragalus polysaccharide and chitosan oligosaccharide. Wherein the lactic acid bacteria accounts for 30%-40%, the bacillus accounts for 20%-30%, the clostridium butyricum accounts for 10%-20%, the fructo-oligosaccharide accounts for 5%-10%, the yeast beta-glucan accounts for 3%-8%, the astragalus polysaccharide accounts for 2%-5%, and the chitosan The probiotics can enhance immunity and regulate intestinal tracts at the same time, the effect is improved by 40% or above compared with common probiotics, animals suffer less illness and grow fast, flora can resist high temperature and gastric acid through a special protection technology, the loss of viable bacteria is less than 5% after the probiotics are stored for 3 months, and the probiotics are stable by two times compared with conventional products.

The invention provides a method for repairing and maintaining an ecological bank protection structure of a water conservancy project. Comprising the following steps: S1, screening and domesticating functional microorganisms; S2, constructing a vegetation and microorganism synergistic remediation unit; S3, precisely arranging the synergistic remediation unit on site; S4, dynamically monitoring and coordinately regulating and controlling: screening and domesticating the functional microorganisms, constructing the synergistic remediation unit, precisely arranging the synergistic remediation unit on site, and dynamically monitoring and regulating and controlling. A microorganism-vegetation-carrier synergistic effect mechanism is formed, so that the technical problems of structural stability and ecological function separation in traditional revetment restoration are solved; the method has the advantages that the synergistic interaction of structure stability and ecological restoration is realized, the self-repairing capability of a microorganism-vegetation symbiotic system is improved, a multi-dimensional dynamic monitoring regulation and control mechanism is established, and the manual maintenance cost is reduced.

The invention discloses a phosphate solubilizing bacterium with eurytopic characteristic and application thereof, the bacterium is separated from corn straw biochar in which rice is continuously planted for 3 years, is identified as Pseudomonas paaversuta and is named as MPP2401, the preservation unit of the bacterium is China Center for Type Culture Collection (CCTCC), the address is Wuhan University, Wuchang District, Wuhan, Hubei province, the preservation date is November 6, 2024, and the preservation number is CCTCC NO: M20242439. The strain can keep stable phosphate solubilizing capacity under the environmental conditions of 15-30 DEG C, pH of 7-10 and NaCl 0-0.8 mol.L <-1 >, and still has functional phosphate solubilizing capacity even under the condition of extreme low temperature of 5 DEG C, the composite stress of pH of 7-8 and NaCl 0-0.8 mol.L <-1 > and the composite stress of pH of 9-10 and NaCl 0-0.6 mol.L <-1 >. Meanwhile, the bacterium has the broad-spectrum antibacterial characteristic, and the antibacterial rate on fusarium graminearum, fusarium verticillioides and magnaporthe oryzae reaches 41.0-77.6%. Due to the unique wide environment adaptability and the phosphate solubilizing-bacteriostasis dual-function synergistic characteristic, a microbial germplasm resource with production-ecology dual values is provided for biochar bacterial fertilizer development, saline-alkali phosphorus activation and biological prevention and control.

The invention belongs to the technical field of microorganisms, and discloses a Lactobacillus paracasei B20 for inhibiting melanin from a plateau area and application of the Lactobacillus paracasei B20, the Lactobacillus paracasei B20 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.32434, and the preservation date is October 31, 2024. Metabolite of the lactobacillus paracasei B20 comprises phenylalanine, p-hydroxycinnamic acid and the like, has strong tyrosinase inhibition effect and anti-ultraviolet ability, and can significantly reduce generation of melanin in skin. In addition, the lactobacillus paracasei B20 also has relatively strong oxidation resistance, has good tolerance in artificial gastric juice and artificial intestinal juice, and can smoothly reach the intestinal tract of a human body. In addition, the lactobacillus paracasei B20 has a relatively obvious growth inhibition effect on skin conditioned pathogens such as staphylococcus aureus and enterococcus faecalis.

The present invention provides a method for producing extracellular vesicles, the method including a step for passing a crushed liquid of plants, mushrooms, or microalgae through a depth filter, and a step for subjecting the crushed liquid after passing through the depth filter to ultrafiltration treatment, the depth filter being composed of filaments.

The invention discloses a lactobacillus rhamnosus strain with antiallergic activity and a preparation method thereof, the strain is lactobacillus rhamnosus Lrn-PG-06, the classification name of the strain is Lactobacillus rhamnosus, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.3077, the preservation time is March 21, 2024, the preservation unit is China General Microbiological Culture Collection Center, the preservation number is CGMCC No.3077, and the preservation number is CGMCC No.3077. The address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The lactobacillus rhamnosus Lrn-PG-06 strain is high in acid resistance, alkali resistance and cholate resistance, high in simulated gastrointestinal fluid resistance and high in oxidation resistance, has the advantage of resisting allergy, and solves the problems that at present, allergic diseases are mainly treated by drugs or are prevented from being in contact with allergens in the environment and food, but due to the fact that the drugs are large in toxic and side effects, the allergens cannot be in contact with the allergens in the environment and food. And allergens causing anaphylaxis are difficult to determine, so that allergic diseases are difficult to treat or prevent.

The present invention discloses a Kluyveromyces marxianus yeast strain, a method for fermenting rose-flavored coffee beans and the coffee beans. The Kluyveromyces marxianus yeast strain is selected as the fermentation strain. By obtaining and preserving microorganisms from sour meat, the green coffee beans are co-fermented with Damascus rose flowers, thereby endowing the coffee beans with a unique rose flavor. The green coffee beans are subjected to high-temperature sterilization and the rose petals are subjected to ultraviolet sterilization treatment; then inoculation is carried out using a fermentation strain with a specific concentration; then multiple fermentations are carried out in a constant temperature and humidity environment to fully integrate the rose aroma and coffee flavor. Through selective fermentation and precise control of the inoculation concentration, it is ensured that the flavor compounds generated during the fermentation process are consistent, achieving a stable flavor performance. The finally obtained rose-flavored coffee has a unique flavor, moderate acidity, good body, balanced sweetness, appropriate roasting degree and uniform particle size. Its moisture content and trace element content are precisely controlled, and the tasting score reaches 81.3 points.

The invention discloses a bacillus subtilis strain and application thereof in prevention and treatment of tomato brown wrinkled fruit virus diseases. According to the bacillus subtilis strain, the bacillus subtilis strain is NA25, the preservation name is bacillus subtilis, the bacillus subtilis strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), the address of the preservation unit is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation date is April 27, 2025; the preservation number of the strain is CGMCC No. 34380. The bacillus subtilis provided by the invention is separated from healthy tomato endophyte, and the strain fermentation liquor and cell suspension both have a good inhibition effect on the brown wrinkled fruit virus, and can be used for preventing and treating the tomato brown wrinkled fruit virus.

The embodiment of the invention discloses a Yarrowia guilliermondii as well as a separation and screening method, an identification method and application of the Yarrowia guilliermondii. The preservation number of the Yarrowia guilliermondii is CGMCC (China General Microbiological Culture Collection Center) No.27603, and the preservation number of the Yarrowia guilliermondii is CGMCC No.27603. The Yarrowia guilliermondii disclosed by the embodiment of the invention has a very good prevention and control effect on postharvest diseases of fruits, and the prevention and control effect of the Yarrowia guilliermondii on the diseases can be enhanced by respectively adding auxiliary factors, namely calcium chloride, alginate oligosaccharide (AOS), phytic acid (PA) and metschhia yeast. Through identification, the antagonistic bacterium is a Yeyrozyma guilliermondii, and the antagonistic bacterium is a Yeyrozyma guilliermondii. The M.guilliermonii has a good control effect on gray mold, penicilliosis, black spot and aspergillus niger of strawberry, apple, pear and tomato fruits, can be rapidly colonized on fruit wounds, and can enhance the resistance-related enzyme activity of the fruits, occupy the wound space and induce the fruit resistance to reduce the morbidity of diseases.

Algae harvesting and cultivating systems and methods for producing high concentrations of algae product with minimal energy. In an embodiment, a dead-end filtration system and method includes at least one tank and a plurality hollow fiber membranes positioned in the at least one tank. An algae medium is pulled through the hollow fiber membranes such that a retentate and a permeate are produced.

The invention belongs to the field of membrane pollution control, and particularly relates to pseudomonas as well as a separation method and application thereof. The invention provides a pseudomonas, which is identified as Pseudomonas knackmussii HITSZ-Q1, is preserved in the Guangdong Microbiological Culture Collection Center, and has the preservation number of GDMCC (China General Microbiological Culture Collection Center) NO: 66162. The strain takes C6-HSL as the unique carbon source and nitrogen source, and the 16S rDNA sequence of the strain is as shown in SEQ ID NO. 1. The strain has a remarkable degradation capability on signal molecules such as C6-HSL, C8-HSL, 3-oxo-C12-HSL and the like, and the highest quenching rate can reach 98.9%. The strain and pseudomonas aeruginosa are co-cultured, so that the signal molecule concentration of the pseudomonas aeruginosa can be effectively reduced, and biofilm formation is inhibited. The strain is further prepared into a carrier in the form of immobilized pellets, the carrier is added into a membrane bioreactor, the operation cycle is prolonged from 6.5 days to 10.5 days, and the membrane pollution delay rate reaches up to 61.5%.

The invention discloses heavy metal resistant bacillus velezensis as well as a microbial remediation agent and application thereof. Bacillus velezensis DBS01 is screened from heavy metal contaminated soil in Dabaoshan mining areas in Shaoguan City, Guangdong province, is preserved in China Center for Type Culture Collection (CCTCC), has a preservation number of CCTCC M 20252228, not only has resistance to various heavy metals, but also can metabolize and secrete metabolites such as organic acid, cellulase and growth hormone in different growth stages, and can be used for preventing and treating various heavy metals. Multiple functions of curing heavy metals, dissolving phosphorus and potassium, promoting plant growth and the like are realized. According to the method, bacillus velezensis DBS01 is inoculated into a culture medium containing straw, calcium superphosphate, agar and sodium alginate are added, and a mixture containing the bacillus velezensis DBS01 is freeze-dried, so that the heavy metal contaminated soil in the mining area can be efficiently repaired in an environment-friendly manner at low cost, and the ecological function recovery of the soil is effectively promoted.

According to the nucleic acid construct based on the flocculation gene and the application of the nucleic acid construct, by replacing a promoter of the flocculation gene or integrating an exogenous flocculation gene into a cell, the cell collection time can be greatly shortened and the energy consumption during cell collection can be effectively reduced under the condition that the overall activity of IVTT of the cell is almost unchanged.

To provide a novel technique for detaching adherent cells from a solid phase.SOLUTION: A cell manipulation method includes: a step of culturing cells in a liquid; a step of disposing a flow path through which a gas is able to be introduced in the liquid; a step of forming an air bubble at an end portion of the flow path; and a step of attaching the cells to the air bubble.SELECTED DRAWING: Figure 10

The invention discloses acetobacter aceti as well as an isolated culture method and application thereof, and belongs to the technical field of microorganisms. According to the invention, an acetobacter aceti SCU-LH-1 strain is separated and screened from vinegar culture, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.33010. The strain provided by the invention can be used for producing litchi kernel table vinegar, lactic acid is preferentially synthesized in the fermentation process, the proportion of organic acid in the table vinegar is changed, and the proportion of lactic acid to acetic acid is remarkably increased, so that the taste of the table vinegar is effectively improved, and the table vinegar is softer and mellower; and the contents of ester components such as isoamyl acetate and lychee flavor components such as geraniol in the table vinegar are increased, so that the table vinegar has richer lychee flavor. According to the method, efficient utilization of fruit byproducts can be achieved, high-quality table vinegar is produced, reasonable utilization of resources is achieved, the raw material source of table vinegar production is expanded, the production cost is reduced, and good economic benefits and environmental benefits are achieved.

The invention belongs to the field of microorganisms, and particularly relates to bacillus Z161 capable of efficiently degrading straw as well as a screening method and application of the bacillus Z161. The bacillus subtilis Z161 is bacillus mexicanus, the preservation number of the bacillus mexicanus Z161 is CGMCC (China General Microbiological Culture Collection Center) NO.36393, and the bacillus mexicanus Z161 is preserved in the China General Microbiological Culture Collection Center on October 29, 2025. The bacillus Z161 screened by the invention has strong laccase activity and high temperature resistance, and can efficiently degrade lignin under the condition of 50 DEG C; the problem that in the prior art, normal-temperature zymophyte is mostly adopted, and the requirement for fermentation conditions in the fermentation process is harsh is solved, and therefore the rapid compost fermentation technology is easier to achieve. The bacillus Z161 provided by the invention has the advantages of genetic and functional character stability, has good tolerance at high temperature, enables lignin to be quickly depolymerized, and improves the decomposition efficiency.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a new enterobacter rorrhizae strain as well as a separation and screening method and application thereof. In order to enrich microbial resources for producing pyrazine substances through metabolism in the white spirit industry, an enterobacter rorrhizae strain is separated and screened from a mixture of yeast for making hard liquor and fermented grains, and the preservation number is CGMCC No.33262. The strain has good tolerance to pH, salt and alcohol, and can adapt to the growth environment in the white spirit production process; and various pyrazine substances represented by tetramethylpyrazine can be produced. Besides, the strain has strong adaptability to fermented grains, the yield of tetramethylpyrazine is high in a culture medium taking bran, wheat and five grains as the fermented grains, and meanwhile, various flavor substances such as ethyl acetate, ethyl hexanoate, ethyl caprylate, 2, 3-butanediol, guaiacol and 4-vinyl guaiacol can be produced. Therefore, the enterobacter rorrhizae disclosed by the invention can be widely used for brewing white spirit.

The invention relates to a low-density polyethylene plastic degrading bacterium, the name of which is Talaromyces fusiformus P6, and the preservation number of which is CCTCC (China Center for Type Culture Collection) NO: M 2025277. The preservation time is February 21, 2025, and the preservation unit is China Center for Type Culture Collection, Wuchang District, Wuhan City, Hubei Province, No. 299. The invention also relates to a separation and screening method of the low-density polyethylene plastic degrading bacterium, a strain capable of degrading low-density polyethylene plastic can be obtained, the strain has a good degrading effect on low-density polyethylene, and after polyethylene is biologically treated for 60 days, the surface of a low-density polyethylene film is colonized by the strain and becomes rough, so that the low-density polyethylene plastic is degraded. And obvious holes and gullies appear. The invention provides a new strain resource for biodegradation of low-density polyethylene plastic, and has a great application prospect.

The invention relates to the field of microorganisms, in particular to lactobacillus plantarum TRT-01 with anti-inflammatory and bacteriostatic functions as well as a preparation method and application of the lactobacillus plantarum TRT-01, the lactobacillus plantarum TRT-01 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.32237, and the preservation date is October 17, 2024. The plant lactobacillus has broad-spectrum bacteriostatic activity on 10 pathogenic bacteria such as helicobacter pylori, streptococcus mutans and the like, and can regulate Th1 / Treg immune balance and relieve inflammatory response by inducing dendritic cells to secrete cell factors IL-10 and IL-12, inducing T cells to secrete cell factors TGF-beta and IFN-gamma and inhibiting secretion of cell factors IL-6 at the same time. Therefore, the plant lactobacillus TRT-01 disclosed by the invention can be used for preparing a composition with anti-inflammatory and bacteriostatic effects, is suitable for the fields of medicines, foods, health-care products and the like, and is particularly suitable for developing a microbial preparation with a probiotic effect.

The invention provides a method for sorting chlorinated organic matter degrading bacteria, and belongs to the technical field of organic matter degrading bacteria sorting.The sorting method comprises the following steps that 1, microorganisms are separated from an environment sample polluted by chlorinated organic matter, and a microbial community is obtained; (2) domesticating the microbial community obtained in the step (1) by using a chlorinated organic matter until the degradation effect of the microbial community on the chlorinated organic matter is stable, so as to obtain a domesticated flora; 3) inoculating the domesticated flora into a heavy water culture medium for culturing to obtain a heavy water labeled flora; and 4) sorting the heavy water labeled flora under the condition of chlorinated organic matter pollution stress by using a single-cell Raman sorting platform, and screening cells with a heavy water labeled characteristic peak and a VB12 characteristic peak as target cells, namely the chlorinated organic matter degrading bacteria. Based on the single-cell Raman sorting technology and in combination with the anaerobic culture technology, the sorting efficiency of the anaerobic chlorinated organic matter degrading bacteria is remarkably improved.

A magnetic bead removal method, device, and storage medium are provided. The method includes the steps of placing a liquid bag having a curved flow path on a magnetic platform, wherein a plane where the curved flow path is located is positioned above the magnetic platform and parallel to the magnetic platform; controlling a first flow rate control assembly to communicate the liquid bag with a sample container, thereby injecting a liquid containing magnetic bead-bound immune cells and non-magnetic immune cells in the sample container into the curved flow path; flattening the liquid bag to increase a contact surface between a bottom surface of the curved flow path and the magnetic platform, and adsorbing immunomagnetic bead cells in the curved flow path onto the contact surface by the magnetic platform; and performing a collection operation to collect non-magnetic immune cells in the curved flow path into a collection container.