814 results about "Beef extract" patented technology

The invention discloses a method for repairing cement-based material cracks, as well as a culture solution and a repair nutrient solution. The method for repairing cement-based material cracks by through microorganisms comprises the following steps that: a Bacillus pasteurii strain is inoculate onto a culture medium provided with a urea-containing substrate; shake cultivation is carried out at a temperature of between 25 and 37 DEG C, and then a culture bacteria solution is taken out and centrifuged and has supernatant fluid removed; strain cells are collected through the culture solution; the concentration of the strain cells is controlled in a range of between 2x10<9> and 1x10<11> cell/ml; standard sand, urea and Ca(NO3)2.4H2O mixture are added to each milliliter of strain cell solution obtained through collection, mixed, stirred into slurry and injected into cement stone cracks; the frequency of the repair nutrient solution injection is not less than two times; finally, maintenance is carried out. In the culture solution, each liter of culture solution contains 4 to 6 g of peptone, 2 to 4 g of beef extract and 20 to 60 g of urea. The method fully utilizes microbial resources in nature; CO3<2-> decomposed out through microbial enzyme can chelate Ca<2+> in a substrate so as to be mineralized and deposit calcium carbonate, and is close in the combination with the substrate and good in stability.

The invention relates to a selenium-rich chitosan nutritional microbial fertilizer. The selenium-rich chitosan nutritional microbial fertilizer is prepared from the following raw materials: sodium selenite, chitosan hydrolysate, compound trace elements, monopotassium phosphate, urea, peptone, yeast powder, NaCl, NaOH, NaNO3, NaCl, NH4Cl, K2HPO4, a beef extract, glucose, starch, calcium carbonate, amino acid, potassium nitrate, ferrous sulfate, magnesium sulfate, trisodium citrate, biochemical enzymes and a mixed bacterium liquid. Due to addition of the chitosan, the viable count of a microbial agent can be kept; the efficacy of the chitosan for keeping the viable count is put into an effective play; due to addition of the chitosan, the amount of bacteria of the microbial agent can reach 20,000,000,000/mL; due to addition of the chitosan to a bacterial manure, the yield of commercial crops can also be increased by 10%-15%; and the nutritional microbial fertilizer has the efficacies of keeping moisture and slowly releasing fertilizer efficiency.

The invention provides bacillus amyloliquefaciens SZ-60 which is characterized in that the collection number is CGMCC No.8277. The bacterial colony formed by the strain on a beef-extract peptone (NB) medium by virtue of single-cell reproduction is irregular with oyster white color, slight upheaval at the center and wet and semitransparent surface; the microscopy is of a rod shape, and the size is (0.3-0.4)*(3.2-3.3)microns; with flagella, G- and spores, the strain can grow on the beef-extract peptone (NB) medium containing 2-5% of NaCl; the formula of the beef-extract peptone (NB) medium contains 3.0g of beef extract, 10.0g of peptone, 5.0g of NaCl, 17g of agar and 1,000ml of water, and the pH is 6.8-7.2. The bacillus amyloliquefaciens realizes a remarkable antagonistic action on the main pathogenic bacteria causing ginseng root rot, epidemic diseases, sclerotinia sclerotiorum, cylindrocarpon destructans, black spot and damping off. The invention also provides an application of the bacillus amyloliquefaciens SZ-60 in preventing plant fungal diseases, or application in preparing a microbial preparation for preventing plant fungal diseases.

The Bacillus amyloliquefaciens HRH 317 strain separated and screened from soil is preserved on March 15th, 2013, and the preservation number is CGMCC No. 7314. The strain is cultured in beef extract-peptone medium, The thalli form a large number of spores, the thalli are rod-shaped before the spores are formed, are arranged individually or in a chain, and are Gram-positive. After cluture for 3 days, the bacterial colonies are milk-white with a diameter of about 6.75mm and with wrinkles on the surfaces, have non-transparent optical characteristic, has projections and are rounded, and the edges are undulant or petaloid. During liquid standing culture, mycoderm is generated. After homology comparison with the 16SrDNA gene sequence of known strains, the homology with Bacillus amyloliquefaciens reaches 99%-100%. The phylogenetic tree of the 16 SrDNA sequence shows that the strain has the highest similarity with Bacillus amyloliquefaciens strain BGP20. After physiological and biochemical identification, the strain is identified as Bacillus amyloliquefaciens.

The invention relates to the technical field of food, and discloses a hot pot base and a preparation method thereof. In the hot pot base, a bovine bone soup powder, bovine bone marrow powder and a beef extract which are rich in bone collagen, various amino acids such as hydroxyproline and the like, vitamins and various trace elements such as calcium, phosphorus, ferrum and the like are added into beef tallow, glutinous rice cake hot pepper, ginger, garlic, broad bean paste, fermented soya beans, table salt, white sugar, spice and monosodium glutamate which serve as raw materials, so that the hot pot base has the abundant nutrition while having fresh and fragrant taste. Compared with the hot pot base in the prior art, the hot pot base has the characteristic of fresh and fragrant taste and nutrition. The hot pot base prepared by the method is peppery but not dry, pungent but not bitter and fresh but not thin, is greased and mellow, has moderate mouthfeel and abundant nutrition, and is suitable to be eaten by people which have different dietary habits in every place.

The invention provides a method for synergistically degrading oil producing wastewater by using petroleum degrading bacteria. The method comprises the following steps: (1) inoculating purified single pseudomonas aeruginosa, bacillus subtilis, pseudomonas mendocina and acinetobacter baumannii strains into a beef extract-peptone medium, and culturing for 18 hours so as to obtain seed bacterium liquid; (2) preparing an acclimation culture solution of the seed bacterium liquid; (3) adding the prepared acclimation culture solution to a fermentation medium according to a single strain bacterium liquid isopyknic ratio, and culturing; (4) putting fermented bacterium liquid in an aeration tank so as to treat the oil producing wastewater. The composite high-efficiency oil removal bacterial strain can be used for degrading more than 94% of hydrocarbon compounds in the oil producing wastewater; the selected strains are respectively high in crude oil degradation rate and have synergistic effect; the combined degradation efficiency of several bacteria for crude oil is considerably higher than the simple superimposed effect of the single effect of the bacteria.

The invention relates to a clear liquid fermentation medium for clostridium butyricum. The clear liquid fermentation medium, the pH value of which is 7-8, is made from glucose, tryptone, a yeast extract powder, ammonium sulfate, sodium bicarbonate, a maize liquid powder and the like. The clear liquid fermentation medium for clostridium butyricum contains metal salts for promoting the growth of gemma, wherein the metal salts are manganese sulfate, magnesium sulfate and ferrous sulphate. The enrichment medium of the clear liquid fermentation medium for clostridium butyricum contains yeast extract, beef extract, tryptone, glucose, soluble starch, sodium chloride, sodium acetate trihydrate, cysteine hydrochloride, methylene blue at the concentration of 0.5% and distilled water; and the pH is adjusted to 7.1. A fermentation culture method of the clear liquid fermentation medium for clostridium butyricum comprises the following steps of: glycerin tube refrigerated strain activation, heating and optimization, Erlenmeyer flask first-order seed culture, liquid fermentation and spray drying. The production of clostridium butyricum has advantages of simple operation, high amount of zymocyte, high gemma yield, simple post-treatment, less impurities in a bacterial powder sample and high amount of effective live bacteria, and saves cost at large.

The present invention provides Bacillus vallismortis SZ-4, which has the preservation number of CGMCC No.8273. According to the present invention, the strain grows on a beef extract-peptone (NB) culture medium in a single cell reproduction growth manner to form bacterial colonies, and the bacterial colonies have characteristics of round shape, milk white color, slightly elevated center, and moist and transparent surface; microscopic examination results show that the Bacillus vallismortis SZ-4 has a rod shape, has flagella, has a size of 0.7-0.82*1.6-2.2 mum, presents the positive Gram staining effect, has spores, and can grow on a beef extract-peptone (NB) culture medium containing 2-5% of NaCl, wherein the beef extract-peptone (NB) culture medium comprises: 3.0 g of a beef extract, 10.0 g of peptone, 5.0 g of NaCl, 17 g of agar and 1000 ml of water, and the pH value is 6.8-7.2; and the Bacillus vallismortis SZ-4 provides significant antagonism effects for pathogenic bacteria causing ginseng root rot, blight, sclerotinia sclerotiorum and botrytis cinerea. The present invention further provides an application of the Bacillus vallismortis SZ-4 in prevention and control of plant fungal diseases or preparation of microorganism preparations for prevention and control of plant fungal diseases.

The invention discloses a soil curing method through carbonate-mineralization microbes, including the following steps: first, preparing high concentration microbe liquid: inoculating Bacillus pasteurii strain into the culture fluid of beef extract and peptone; wherein each litre of culture fluid contains 4-6g peptone and 2-4g beef extract; cultivating for16-24h under30DEG C with the pH value controlled at 6-8, then getting out the mixture and centrifuging for 5-8min under the speed of 5000-8000rpm, thus getting high-concentration microbe liquid at concentration of 2 X 10<9>-2 X 10<11>cell/ml; second, mixing the soil: mixing the high-concentration microbe liquid prepared in the first step and the newly prepared culture fluid into the dried soil sample through lime(3%-7% the weight of the dry soil) and carbamide(2%-6% the weight of the dry soil) according the proportioning of 100g dry soil per 2-3mL high-concentration microbe liquid and 0-50mL newly prepared culture fluid, blending to be even and then cultivating for 0.5-8days; third; molding through a die: molding the soil mixture cultivated in second step in a die when the mixture reaches the optimal water ratio, then demoulding and conserving in constant temperature of 20 DEG C. Seven days later, the soil will be cured, with the compressive resistance reaching above 1.0MPa, up by 38% as compared with pure soil.

The invention discloses a culture medium for improving prevention and control effects of biological control yeast on fruit diseases by induction, which comprises an activation culture medium and a seed culture medium, wherein the activation culture medium is prepared into NYCA activation culture medium from 8 grams of beef extract, 5 grams of yeast powder, 2 to 30 grams of glucose, 20 grams of agar and 1 to 50 grams of chitin by fixing volume to 1,000 milliliters with water and sterilizing the mixture under high pressure; and the seed culture medium is prepared into NYCB seed culture medium from 8 grams of beef extract, 5 grams of yeast powder, 2 to 30 grams of glucose and 1 to 50 grams of chitin by fixing volume to 1,000 milliliters with water and sterilizing the mixture under high pressure. The invention also discloses a method for improving the prevention and control effects of the biological control yeast on the fruit diseases by induction by using the culture medium at the same time. The method comprises steps of performing activation and liquid culture and the like on cryptococcus laurentii CGMCC No.3590.

The invention discloses a powdery composite seasoning with beef flavour, which comprises the following components in part by weight: 20 to 40 parts of monosodium glutamate, 25 to 45 parts of salt, 1 to 10 parts of sugar, 1 to 15 parts of dextrin, 5 to 20 parts of starch, 0.3 to 0.8 part of curry powder, 0.0005 to 0.0015 part of spice, 0.0003 to 0.002 part of essential oil of beef, 0.003 to 0.008 part of beef powder, 0.003 to 0.008 part of beef extract, 0.003 to 0.005 part of vegetable oil, 0.0003 to 0.001 part of caramel, 0.0003 to 0.001 part of onion granules, 0.0002 to 0.001 part of carrot granules and 0.0002 to 0.001 part of celery granules. The powdery composite seasoning is appetizing and tasty, and has the effects of strengthening spleen and stimulating appetite, and nourishing and protecting health.

The invention discloses a brine sauce seasoning packet suitable for non-fried miscellaneous-grain instant noodles. The seasoning packet comprises a sauce packet, and a powder packet. The sauce packet comprises the raw materials of: beef, salad oil, butter, palm oil, white sugar, water, old ginger, green onion, garlic, star anise, cumin, fructus amomi, tsao-ko amomum fruit, dried tangerine peel, cinnamon, licorice, white buckle, rose pelargonium herb, angelica archang lica, kaempferiae, flatspine pricklyash, clove, whitefruit amomum fruit, edible salt, monosodium glutamate, I+G, ethyl maltol, maltodextrin, a yeast extract, HVP, a beef extract paste, and thermal reaction beef powder. The powder packet comprises the raw materials of: edible salt, monosodium glutamate, white granulated sugar, I+G, HVP, black pepper powder, creamer, yeast extract, beef powder, and turmeric powder. A dried vegetable packet comprises the raw materials of: Chinese cabbage, carrot, green onion, and caraway. The seasoning packet provided by the invention has the advantages of distinctive flavor, rich fragrance, and delicious taste. The production method is simple and is easy to command, the technology is mature, and the method provided by the invention is suitable for popularization.

The invention discloses a bacillus cereus fermentation method for producing phosphatidase C and belongs to the technical field of enzymic catalytic reaction. The bacillus cereus fermentation method for producing phosphatidase C is utilized to solve the problem of bacterial strains used in the production of phosphatidase C and the problem of complex technology, and comprises the following steps of: inoculating bacillus cereus into the LB medium for culturing to obtain a seed liquid; vaccinating the seed liquid in a fermentation medium consisting of beef extract, peptone, NaCl, water, zincsulfateheptahydrate or ZnCl2, and lecithin or a phosphatide mixture which is the mixture of PC, PE, PI, PS and the like, for culturing to obtain a fermentation broth; and separating the fermentation broth to obtain phosphatidase C. The bacillus cereus fermentation method for producing phosphatidase C can be widely applied to the production of phosphatidase C for plant oil degumming.

The invention discloses a chromogenic medium for detecting salmonella, which belongs to the fields of food safety and clinical microbiological detection. The culture medium contains agar, peptone, beef extract powder, sodium chloride, a surfactant, cholate, an enzyme inducer, an octoate enzyme chromogenic substrate, a beta-galactosidase chromogenic substrate, a hexosaminidase chromogenic substrate and robiocina. The chromogenic medium disclosed by the invention is used for detecting salmonella, has high detection sensitivity and high specificity, and can be used for initially identifying strains directly according to the color of a colony; the chromogenic medium has high operability, and suitable for treating large-reflux samples, and can be used for comprehensively, systematically and accurately detecting and initially identifying salmonella in food production, clinical disease diagnosis and the environment; and a new way is provided for rapid detection of microorganisms.

The invention discloses a processing method of a viable clostridium butyricum preparation and a culture medium composition. The processing method comprises the following steps: carrying out activation and enlarge cultivation on strains; culturing the strains by utilizing a seeding tank; carrying out fermentation cultivation by utilizing a fermentation tank, wherein a fermentation culture medium comprises the following components: corn flour, peptone, beef extract powder, yeast extracts, dipotassium phosphate, monopotassium phosphate, calcium chloride, magnesium sulfate, manganese sulfate, ferrous sulfate, sodium acetate and cysteine salt. According to the processing method, by optimizing the culture medium and a processing process, the fermentation cultivation is short, and the maturing rate of spores is high; furthermore, a thallus collection method is further optimized, so that the production process is greatly simplified, the energy consumption can be substantially reduced, and the yield of active thalli can be improved.

The invention discloses a biocontrol agent for preventing and curing bacterial wilt and club roots of a plant as well as preparation method and application thereof. The preparation method of the biocontrol agent comprises the following steps: inoculating activated bacillus amyloliquefaciens ZJUB2008 into a culture solution, and fermenting and culturing at 25-45 degree C for 6-120 hours, wherein the culture solution comprises the following components by taking the volume of 1L as the basis: 3-15g of peptone, 0-3g of beef extract, 0-5g of glucose, 0-5g of yeast powder, 0-8g of NaCl, and the balance of water; and the pH value of the culture solution is 5.5-9.0. According to the invention, the bacillus amyloliquefaciens ZJUB2008 is used to prepare the biocontrol agent on/under the appropriate fermentation medium and culture conditions by means of microbial fermentation; in addition, the prepared biocontrol agent has a better prevention effect to different chemotyped ralstonia and plasmodiophora, can remarkably lowering the occurrence rate and severity of the bacterial wilt and the club roots, and is suitable for large-scale production.

The invention discloses a methylotroph bacillus and application thereof to preparation of a plant pathogenic bacterium sterilization agent. The methylotroph bacillus CGMCC No.13541 has the antagonism effect on various phytopathogens such as botrytis cinereal, fusarium graminearum and rhizoctonia solani, and belongs to an environment-friendly antagonistic strain with the obvious plant disease prevention and control effect; the development and application prospects are good. Culture liquid obtained through culture by a beef extract liquid culture medium can be used for preventing and controlling plant diseases. The bacteriostasis rate on the botrytis cinereal is as high as 84 percent; the value is about 8 percent higher than that of ordinary biocontrol bacteria; the foundation is laid for the novel disease prevention microbe resource development and utilization. The resistance capability is high; the use range is wide; the growth speed is high; fast and great-amount multiplication can be realized; the popularization and application of a biological prevention and control preparation in the field are convenient.

The invention provides a microecological preparation with bacillus subtilis fermenting astragalus membranaceus and an application thereof, wherein the microecological preparation is prepared through fermentation by means of a bacillus subtilis strain FM537 on a fermentation substrate containing astragalus membranaceus water extract liquid. The fermentation substrate comprises 15 g/L of tryptone, 15 g/L of glucose, 50 g/L of beef extract, 0.5 g/L of ammonium sulfate, 1 g/L of sodium chloride and 0.5 g/L of sodium dihydrogen phosphate. The fermentation substrate is prepared with distilled water, wherein pH is regulated to 7.2, and then according to volume ratio, 45-50% of the astragalus membranaceus water extract liquid is added, and finally high-pressure sterilization is carried out to produce the fermentation substrate. Through ultrasonic wave for assisting water extraction of the astragalus membranaceus, the extraction time is greatly reduced. Through selection of suitable fermentation temperature and fermentation time, synergistic effect of the astragalus membranaceus and the bacillus subtilis is improved. The microecological preparation is used as a livestock and poultry feed additive, and can improve animal production performance, digestion rate and body immunity.

The invention discloses a bacterial cellulose fermentation medium made from apple pomace and a method for producing the bacterial cellulose by utilizing the medium, and belongs to the field of biotechnology. The bacterial cellulose fermentation medium comprises cane sugar, beef extract, disodium hydrogen phosphate, citric acid, ethanol and apple pomace hydrolysate. The method comprises the following steps: (1) activating and propagating gluconacetobacter xylinum to obtain gluconacetobacter xylinum seed solution, incubating the gluconacetobacter xylinum seed solution into a bacterial cellulose fermenting medium for fermenting to obtain bacterial cellulose fermenting solution; and (2) treating a bacterial cellulose in the bacterial medium fermenting solution to obtain bacterial cellulose. The method has the advantages of low cost, simple process and the like, is convenient for industrial production, is good in stability and reproducibility, and is suitable for large-scale production, wherein the yield of the fermented bacterial cellulose is more than 15g/L.

The invention relates to a method for recovering rare earth from waste cathode ray tube fluorescent powder through microbial leaching. The method is characterized by comprising the steps of (a), performing pretreatment: drying fluorescent powder until constant weight is achieved, and grinding the dried fluorescent powder with constant weight into fine powder with a grinding instrument for standby application; (b), performing microbe selection and culturing: selecting funguses or bacteria as leaching microbes, and selecting liquid cane sugar culture mediums and beef extract peptone culture mediums as culture mediums for separately culturing the funguses and the bacteria; (c), performing leaching treatment: adding levigated fluorescent powder into the culture mediums containing strains, putting the culture mediums with the levigated fluorescent powder into a vibrating culturing box, performing culturing for some time, performing filtration, obtaining waste slags, collecting thalli, and decomposing the thalli so as to obtain a leaching solution containing rare-earth elements. Through the adoption of the method, not only can the innocent treatment of the waste fluorescent powder be realized, but also rare earth resources can be recovered.

The invention provides a fluorogenic culture medium of Enterobacter sakazakii, a test kit and a test method thereof, and relates to a method for testing microbe and a composition used by the method. The fluorogenic culture medium comprises the compositions as follows: 8 to 20 grams of peptone, 4 to 7 grams of beef extract powder, 4 to 7 grams of sodium chloride, 1.0 to 2.0 grams of cholate, 12 to 20 grams of agar, 0.05 to 0.5 grams of X-a-glucopyranoside, 0.01 to 0.04 grams of Na2CO3, 0.3 to 2.0 grams of ferric ammonium citrate, and 0.3 to 2.0 grams of sodium thiosulfate. The test kit consists of the fluorogenic culture medium of Enterobacter sakazakii, an enriched liquid A ,namely buffer peptone water culture medium, and an enriched liquid B, namely modified lauryl sulfate pancreas peptone broth. The test kit is configured simply, and the test method has high sensitivity in detection, so the method is suitable for treating large flux samples.

The invention discloses a chromogenic medium used for detecting esherichia coli O157:H7. The medium comprises agar, peptone, beef extract powder, sodium chloride, sorbitol, inositol, neutral red, beta-galactosidase chromogenic substrate, beta-glucuronidase chromogenic substrate, isopropyl-beta-D- thiogalactopyranoside, natrium taurocholicum, potassium tellurite and cefixime. The chromogenic medium used for detecting esherichia coli O157:H7 of the present invention has the advantages of high sensitivity, good specificity, direct bacterial strain discrimination according to colony color, short detection period and strong operationality, is suitable for treating high-reflux samples, is capable of comprehensively, systematically and accurately detecting and preliminarily identifying the esherichia coli O157:H7 in food production and environment, and provides a novel approach for rapidly detecting the microbes.