43 results about "Beef extract" patented technology

The invention relates to the technical field of fermentation and purification of clostridium histolyticum collagenase, and discloses a fermentation and purification method of clostridium histolyticum collagenase, which comprises the following steps: step 1, clostridium histolyticum is inoculated into an enrichment medium according to an inoculum size of 2%, the enrichment medium comprises 1g / L of beef extract, 1g / L of peptone, 1g / L of yeast powder, 3g / L of gelatin and 100mL of ultrapure water, and the pH value is 7.4-7.6; step 2, adding 50.5 [mu] L of Fe < 2 + > and 500 [mu] L of Ca < 2 + > into the enrichment culture medium; step 3, performing fermentation culture for 24 hours at 37 DEG C under an anaerobic condition, and performing centrifugation to obtain fermentation supernate. By determining 37 DEG C as the optimal fermentation temperature, 1% nitrogen source concentration and 24-hour fermentation time and innovatively introducing 2.8 mg / 100mL Fe < 2 + > and 10.0 mmol / L Ca < 2 + > as key additives, the clostridium histolyticum collagenase with high expression quantity and high enzyme activity is obtained, the collagenase activity (A-345 <->) is increased from 0.790 U / L to 0.903 U / L, meanwhile, the high protein concentration of 40-45 mg / mL is kept, and the clostridium histolyticum collagenase is suitable for industrial production. Liver and gall tissues of mice can be digested in a short time, and bile duct cells are extracted for organoid culture.

The invention discloses a method for screening and cultivating a bolete mutant strain, which belongs to the field of microorganisms, and mainly comprises the following steps: obtaining the bolete mutant strain by using a carbon ion beam irradiation technology, naming the bolete mutant strain as ng2, and preserving the bolete mutant strain with the preservation number of CCTCC (China Center for Type Culture Collection) M 2025077; inoculating the ng2 strain into a hypha rapid germination and propagation solid culture medium PDA or PDA + peptone + beef extract + yeast extract powder, wherein hypha begins to germinate after 4 days; the method comprises the following steps: inoculating a solid strain into a hypha rapid propagation liquid culture medium including cane sugar, corn flour, soybean flour, wheat bran, monopotassium phosphate and yeast extract powder, and generating a large amount of fungus balls within 7 days; inoculating a liquid strain into a fruiting culture raw material self-made fermentation material, corn straw, turfy soil and rice, wherein hyphae overgrow in 30 days; the ng2 strain is high in activity, high in growth speed and suitable for being used as an excellent bolete strain in the Heilongjiang region; the screening and cultivating method of the bolete mutant strain is mainly used for solving the problems that the propagation efficiency of the bolete strain is low, germplasm resources in northern areas such as Heilongjiang are deficient and the like.

This invention discloses an improved PMA culture medium for Agaricus bisporus mother culture and its application, belonging to the field of edible fungi cultivation technology. The culture medium of this invention comprises PMA medium, Agaricus bisporus substrate residue, and peptone or beef extract. Experiments show that adding Agaricus bisporus substrate residue to the PMA medium significantly promotes mycelial growth. Further addition of a specific amount of peptone or beef extract produces a synergistic effect, increasing the mycelial growth rate to over 6.53 mm / day, and shortening the mother culture cycle from the traditional 23-25 ​​days to approximately 13-15 days. This invention improves the efficiency of spawn production, reduces production costs and pollution risks, and realizes the resource utilization of substrate residue.

The invention relates to the technical field of Escherichia coli detection, and particularly discloses a semisolid culture medium for rapid detection of Escherichia coli and a detection method and application thereof, the semisolid culture medium comprises the following components by weight: 4.2-5.8 g / L of agar; 8.5 to 11.5 g / L of tryptone; the semi-solid culture medium comprises the following components: 3.2 to 4.8 g / L of beef extract, 6.5 to 9.5 g / L of lactose, 1.2 to 2.2 g / L of D-glucose, 4.2 to 5.8 g / L of sodium chloride, 42000 to 58000 [mu] g / L of penicillin, 0.12 to 0.28 g / L of cholate, 0.06 to 0.14 g / L of disodium EDTA (Ethylene Diamine Tetraacetic Acid), 0.12 to 0.22 g / L of L-cysteine, 1.2 to 2.8 g / L of yeast extract, 0.005 to 0.015 g / L of vitamin B and an acid-base indicator, the semisolid culture medium and the detection method thereof provided by the invention not only solve the core pain point of the existing escherichia coli detection method in the technical level, but also reduce the operation cost and improve the benefit for enterprises in the economic level, promote the standardization and high efficiency of the food microorganism detection technology in the industry level, and have practicability and industrial popularization value.

The utility model belongs to the technical field of microorganism culture dish sampling, and particularly relates to a microorganism culture dish sampling device which comprises a sleeve, the sleeve is made of a transparent plastic material, a fixing plate is installed on the top side of the sleeve, a rod seat is installed on the fixing plate, a lead screw penetrates through the rod seat, a connecting plate is fixedly installed on the bottom side of the lead screw, and the connecting plate is fixedly connected with the sleeve. The bottom side of the connecting plate is fixedly provided with a sampling pipe, the sampling pipe is assembled in the sleeve, the bottom side of the limiting plate is provided with a mounting plate, the mounting plate is provided with a reed, the sampling pipe is pushed by a lead screw to be inserted into a beef extract peptone solid of a microorganism culture dish, and after a sample is filled into the sampling pipe, the sampling pipe is inserted into the reed. The sampling tube drives a sample to be pulled out of the microorganism culture dish, the reed scrapes off solids on the outer wall of the sampling tube, and finally the sampling tube moves back into the sleeve, and according to the structure, the sampling tube is internally arranged, and the outer wall of the internally arranged sampling tube is relatively clean, so that the pollution of the sampling tube to the microorganism culture dish is reduced.

The invention belongs to the technical field of microorganisms, and discloses a high-density fermentation process of vagina-sourced lactobacillus crispatus. The invention discloses a fermentation medium which comprises glucose, tryptone, beef extract, yeast extract, dipotassium phosphate, ammonium citrate, L-cysteine or salt thereof, magnesium sulfate and manganese sulfate. According to the fermentation culture medium provided by the invention, through scientific compounding of a carbon source, a nitrogen source and key factors, the final viable concentration and thallus activity of vagina-derived lactobacillus crispatus can be remarkably improved. The fermentation culture medium is adaptive to the micro-aerobic metabolism characteristics of vagina-derived lactobacillus crispatus by precisely regulating and controlling the dissolved oxygen level (initial dissolved oxygen is controlled to be 5% or below), and when the fermentation culture medium is used for culturing lactobacillus crispatus, the fermentation delay period can be remarkably shortened to 2-3 h, the final viable bacterium yield is greatly increased to 1 * 10 < 10 > CFU / mL or above, and the production cost is reduced. And an efficient and stable technical support is provided for industrial production of the probiotic preparation.

The invention relates to the technical field of decay accelerators, in particular to a composite organism-based soil improvement decay accelerator and a preparation method thereof. The composite organism-based soil improvement decay accelerator is prepared from the following raw materials in parts by weight: 20 to 30 parts of molasses, 5 to 10 parts of eggs, 3 to 8 parts of beef extract, 4 to 12 parts of fresh milk, 6 to 15 parts of crushed trash fish, 2 to 5 parts of urea, 1 to 3 parts of monopotassium phosphate, 0.4 to 0.6 part of fungicide, 1 to 3 parts of tea saponin and 6 to 8 parts of additive. According to the invention, the sodium alginate-vermiculite porous carrier structure of the additive is utilized to realize physical embedding and slow release of the pesticide, the major technical contradiction that viable bacteria and the pesticide are difficult to be compatible is effectively solved, and meanwhile, the carrier integrates the functional components such as humic acid and calcium carbonate, so that the carrier has a good application prospect. The comprehensive effects of accelerating decomposition, preventing and controlling plant diseases and insect pests and improving the soil structure can be synchronously realized after the decomposition accelerant is applied, and the defects that a traditional decomposition accelerant is single in function and needs to be applied for multiple times are overcome.

To provide a meat extract seasoning suppressed in generation of foreign taste and odor, reduced in odor peculiar to livestock, and excellent in flavor, and to provide a method for producing the meat extract seasoning.SOLUTION: The meat extract seasoning contains a raw material meat extract (beef extract, pork extract, chicken extract, etc.) and 0.5 to 5% by weight of a Maillard reaction product derived from 1, 5-D-anhydrofructose obtained by heating a sugar solution containing 1, 5-D-anhydrofructose at a temperature in the range of 60 °C to 150 °C for 30 seconds to 100 hours.SELECTED DRAWING: None

The invention relates to the technical field of environmental engineering, in particular to a photo-thermal evaporation material for salted loess site remediation and a preparation method and application thereof. The preparation method comprises the following steps: dispersing modified zeolite on the surface of a polypropylene non-woven fabric, sequentially spraying a pyrrole aqueous solution and a ferric chloride aqueous solution, continuously and repeatedly coating the modified zeolite, spraying the pyrrole aqueous solution and the ferric chloride aqueous solution, and performing in-situ polymerization to form a cross-linked coating; the preparation method comprises the following steps: preparing a spore suspension from beef extract, peptone, a manganese sulfate titanium dioxide solution, a nickel chloride hexahydrate solution, a yeast extract, an ammonium chloride solution and a sodium chloride solution, sequentially soaking a polypropylene non-woven fabric with a cross-linked coating formed on the surface into the spore suspension and a sodium alginate-graphene oxide mixed solution, spraying a calcium chloride solution, and drying to obtain the polypropylene non-woven fabric with the cross-linked coating formed on the surface. The photo-thermal evaporation material has the advantages that the photo-thermal conversion capability and the light absorption rate are excellent, the moisture evaporation rate is high, and the salt content of the salted loess can be obviously reduced in a short time.

The invention relates to the technical field of porous material preparation, in particular to microorganism-gel synergistic reinforced fly ash ceramsite and a preparation method thereof. The invention relates to a culture medium for culturing bacillus Coriolis, which takes a circulating fluidized bed fly ash leaching solution as a solvent and comprises the following components: 5-10g / L peptone, 3-5g / L beef extract, 0.42-0.5 g / L sodium bicarbonate and 0.53-0.65 g / L sodium carbonate. According to the method, fly ash ceramsite is prepared from the bacillus Coriolis cultured by the culture medium, lightweight non-fired ceramsite with high porosity can be prepared, and a theoretical model and an experimental example are provided for development of a low-carbon, low-energy-consumption and high-added-value solid waste recycling technology.

The application discloses an improved PDA culture medium for Agaricus bisporus mother culture and application and belongs to the technical field of edible mushroom cultivation. The improved PDA culture medium for Agaricus bisporus mother culture is based on a PDA culture medium and is added with Agaricus bisporus residue and peptone or beef extract. The culture medium provided by the application can significantly promote the growth of Agaricus bisporus mycelium, shortens the mother culture time from 23-25 days of the traditional method to about 13-15 days, greatly improves the strain production efficiency, and reduces the production cost and pollution risk.

The utility model relates to the technical field of food processing, in particular to a beef grinder for preparing beef extract, which comprises an upper processing box body, a fixing shell is fixedly connected to the middle of the upper end of the upper processing box body, a driving device is fixedly connected to the middle of the upper end of the fixing shell, and a limiting groove is formed in the middle of the rear end of a lower processing box body. Supporting legs are fixedly connected to the four corners of the lower end of the lower treatment box body, lower plates are fixedly connected to the left portion and the right portion of the lower end of the lower treatment box body, and extrusion devices are fixedly connected between the left ends and the right ends of the two lower plates. According to the beef crusher for preparing the beef extract, the driving device and the crushing assembly are cooperated, the crushing efficiency and the granularity uniformity are improved through the inclined crushing cutter and the blade-replaceable crushing assembly, meanwhile, the problem that blades of existing equipment are prone to abrasion is solved, and the durability of the blades and the adaptability of the equipment to different beef textures are enhanced.

This invention provides a direct-inoculation lactic acid bacteria starter culture, its preparation method, and its application. The method includes: inoculating activated lactic acid bacteria strains into a nucleotide medium for expansion culture to obtain a high-density lactic acid bacteria inoculum; centrifuging the high-density lactic acid bacteria inoculum, mixing the bacterial cells with a freeze-drying protectant, and freeze-drying to obtain the direct-inoculation lactic acid bacteria starter culture; wherein the nucleotide medium comprises: 0.3–0.8 g / L nucleotides, 2–10 g / L beef extract powder, 25–35 g / L soybean peptone, 10–17 g / L sucrose, 1–6 g / L anhydrous sodium acetate, 2–8 g / L triammonium citrate, 1.0–1.6 g / L potassium dihydrogen phosphate, 0.1–0.8 g / L magnesium sulfate monohydrate, 0.023–0.029 g / L manganese sulfate, and 2–6 g / L Tween 80; the nucleotides include at least one selected from guanine, adenine, xanthine, or uracil. The method provided by this invention can improve the activity and stress resistance of lactic acid bacteria strains, and enhance their adaptability to harsh environments, thereby obtaining a direct-inoculation lactic acid bacteria starter with high density and freeze-drying survival rate.

The invention discloses a culture medium for promoting intestinal colonization of probiotics. Every 1000 parts by weight of water of the culture medium is prepared from the following components in parts by weight: 2 to 10 parts of casein, 4 to 12 parts of yeast extract powder, 10 to 12 parts of beef extract powder, 1.5 to 2.5 parts of trisodium citrate or triammonium citrate, 4.5 to 5.5 parts of sodium acetate, 0.1 to 0.3 part of magnesium sulfate heptahydrate, 0.05 to 0.1 part of manganese chloride and 1 to 3 parts of dipotassium phosphate. When the culture medium is used for culturing the probiotics, the secretion amount of exopolysaccharides, proteins and other substances can be remarkably increased, a protective layer is formed, the stress resistance of the strain is improved, the survival ability of the probiotics in a complex gastrointestinal tract environment is improved, and a new strategy is provided for development of the oral probiotics.

The present invention discloses a Vibrio vulnificus selective culture medium and a preparation method thereof. The culture medium comprises a basal culture medium and additives; the basal culture medium comprises peptone, beef extract powder, sodium chloride, potassium chloride, a long-chain alkyl sulfate anionic surfactant or nisin, D-cellobiose, an acid-base indicator, and agar; the additive is tellurite; and the solvent is water. When the basal culture medium contains nisin, the additives further comprise vancomycin, amphotericin B, and polymyxin E. The Vibrio vulnificus selective culture medium of the present invention has high growth selectivity, effectively inhibits the growth of Gram-positive bacteria, effectively inhibits fungal growth, and is sensitive and specific for detecting Vibrio vulnificus. Furthermore, the preparation method does not require autoclaving and avoids the inconvenience of preparing the high-content component, cellobiose, into a sterile additive prior to use, making it more convenient.

The invention belongs to the field of functional ointment preparation, and particularly relates to medicinal and edible functional ointment for preventing and treating senile sarcopenia and a preparation method of the medicinal and edible functional ointment. Chinese herbal medicine powder obtained through powdering is mixed with whey protein powder, beef extract, pea protein powder and a mold fungicide for fermentation, and muscle synthesis and repair components obtained through adsorption of muscle cell absorption promoting colloids can efficiently promote muscle synthesis and repair; the lactide, the glycolide, the stannous octoate and the dichloromethane are mixed to react to form a polymer, the composition for improving the nerve cell function and the hormone secretion regulating component are mixed, and the nerve cell regulator obtained by spray drying can be endocytosed by small intestine cells; the anti-inflammatory mixture capable of effectively inhibiting inflammatory reaction is mixed with the composition for preventing and treating senile sarcopenia, and the obtained medicinal and edible functional ointment for preventing and treating senile sarcopenia is obvious in senile sarcopenia prevention and treatment effect, high in bioavailability, capable of playing a role for a long time and stable in efficacy in the storage process.

The grinding tool for preparing the flavor type beef extract comprises a grinding barrel, the top of the grinding barrel is movably connected with a feeding cover through a rotating shaft, a grinding mechanism is arranged in the grinding barrel, and the grinding mechanism comprises a grinding disc, a grinding motor, a protective cover and an extension rod. The bottom of the extension rod is fixedly connected to the top of the protective cover, the grinding motor is fixedly connected to the interior of the protective cover, the top of the grinding disc is fixedly connected to the output end of the grinding motor, a separation net is fixedly connected to the interior of the grinding barrel, and the radian of the separation net is the same as that of the grinding disc. Through the grinding mechanism, when internal raw materials are ground, a telescopic air cylinder is started, the telescopic air cylinder pushes a protection plate to move downwards, a grinding disc makes contact with the raw materials, the raw materials are ground through rotation of the grinding disc, and the raw materials are fully ground; and by arranging the separation net, the qualified ground raw materials enter the bottom of the grinding barrel through the separation net.

The invention provides a butyrate probiotic culture medium, comprising the following components: 6-12 g / L of peptone, 6-12 g / L of beef extract, 1-5 g / L of yeast extract, 4-8 g / L of glucose, 4-8 g / L of sodium chloride, 0.5-2 g / L of soluble starch, 1-5 g / L of sodium acetate, 0.1-1.0 g / L of L-cysteine hydrochloride, 4-8 g / L of xylan, 1-3 g / L of dipotassium hydrogen phosphate, 0.1-1.0 g / L of magnesium sulfate heptahydrate, and 0.01-0.05 g / L of ferrous sulfate. The invention also specifically provides a method for culturing a high-butyrate cooperative system of Lactobacillus plantarum and Clostridium tyrobutyricum using the culture medium. The method not only improves the butyrate yield but also reduces the production cost of butyrate bioconversion, thereby having significant economic benefits.

The invention discloses a method for removing an organic pollutant bisphenol A, and belongs to the technical field of water treatment. The removal method comprises the following steps: (1) inoculating pseudomonas chlororaphis into a culture medium taking waste molasses as a main raw material, carrying out shake culture at 34-36 DEG C, culturing for at least 24 hours by using the culture medium comprising 1.3-1.4 g / L of waste molasses, 2-4 g / L of beef extract and 4-6 g / L of sodium chloride, centrifuging, and taking supernatant to obtain a culture solution; and (2) adding the culture solution in the step (1) into an anode chamber of a microbial fuel cell reactor, diluting with wastewater, and adjusting the COD content to 1950-2050mg / L. According to the invention, the degradation efficiency and the electricity generation performance of the system on bisphenol A can be obviously enhanced.

The invention discloses a biofilm state lactobacillus culture method for improving the survival rate of lactobacillus, and belongs to the technical field of strain culture. The culture method comprises the following steps: preparing a liquid culture medium; the liquid culture medium contains peptone, beef extract powder, yeast extract powder, glucose, dipotassium phosphate, sodium acetate, magnesium sulfate and manganese sulfate; inoculating lactobacillus into the liquid culture medium, and activating bacterial strains at constant temperature under an anaerobic condition to obtain bacterial suspension; inoculating the bacterial suspension into a culture plate containing the liquid culture medium, and carrying out gradient heating incubation in an anaerobic environment; after incubation is finished, floating bacteria are removed, and biofilm state lactobacillus is obtained through collection. The low-cost raw materials and simple and convenient preparation steps are adopted, the remarkable film forming effect is achieved, the biofilm yield is high, the survival rate of the finally obtained biofilm state lactobacillus in the gastric acid intestinal juice environment can be remarkably increased, and the biofilm state lactobacillus has high practical value.

The object of the present invention is to provide a flavor-enhancing composition that can enhance the flavor of food and beverages with a small amount of addition, and more specifically, to provide a composition that can efficiently enhance the savory flavor of meat, the flavor of stewed dishes, and other cooked flavors and richness. [Solution] The present inventors have conducted various studies to solve the above problem and have found that the above problem can be solved by preparing a composition obtained by heating a mixture of (a) beef extract, (b) glucose, and (c) fructose that satisfies "a:(b+c)=1:1.2~5", "b:c=1:0~4", and "60% by weight ≤ a+b+c ≤ 77% by weight" (where a, b, and c are all solid content equivalent values).

This invention belongs to the field of turfgrass and forage grass maintenance technology, specifically relating to a microbial selenium protein preparation for alleviating drought stress in perennial ryegrass, its preparation method, application method, and application. The microbial selenium protein preparation comprises the following raw materials in specific weight proportions: peptone, beef extract, sucrose, salt, selenium-enriched mushroom powder, organosilicon, calcium amino acids, potassium humate, betaine, salicylic acid, and Bacillus natto. This microbial selenium protein preparation aims to solve the limitations of traditional drought-resistant methods and the poor effectiveness of single-component preparations. When using this microbial selenium protein preparation, it is first diluted and then applied as a root drench to ryegrass seedlings multiple times. The selenium protein preparation of this invention, through the synergistic effect of multiple components, can significantly improve the drought resistance of ryegrass. Furthermore, the preparation process of this protein preparation is simple, environmentally friendly, and efficient, suitable for ryegrass propagation and maintenance, and has broad application prospects.

The invention discloses a culture medium for shortening a bacterial culture delay period and a preparation method and application thereof, and belongs to the technical field of bacterial culture.The adopted rice washing water is rich in nutrient elements such as carbon, nitrogen and phosphorus, and sufficient nutrient substances are provided for shortening the delay period in the bacterial propagation process. The rice washing water is a substance necessary for cell growth and cell composition to participate in cell metabolism; tryptophan is rich in salts and growth factors required by growth of thalli, so that growth and metabolism of the thalli are enhanced; vitamin E is used as a fat-soluble vitamin, is an antioxidant, and can prevent unsaturated fatty acid in phospholipid of a biological membrane from being oxidized; peptone provides a nitrogen source and microorganisms for the microorganisms; the beef extract provides a carbon source, a nitrogen source, phosphate and vitamins for microbial growth; sodium chloride provides inorganic salt to regulate osmotic pressure.

The invention relates to the technical field of pathogenic bacteria culture and preservation. The invention provides a culture medium and a preservation solution for pathogenic bacteria of soft rot of konjak and a use method of the culture medium and the preservation solution. The culture medium is prepared from the following raw materials in parts by weight: 2.5-3.5 parts of beef extract, 9-11 parts of peptone, 4.5-5.5 parts of sodium chloride, 4-6 parts of agar, 4-6 parts of whole konjak powder and 950-1050 parts of water. According to the invention, a konjac soft rot pathogen culture medium formula and a preservation method are improved by utilizing an interaction relationship between konjac soft rot pathogens and konjac. The amorphophallus konjac whole powder is used for replacing part of agar powder and other nutrient substances, the soft rot pathogenic bacteria can be efficiently cultured, and meanwhile, the preservation time of the strain can be remarkably prolonged in cooperation with a specific preservation solution and a preservation method.

The application discloses a culture medium for improving production of paracoccus astaxanthin and application thereof. The culture medium comprises the following components according to concentration calculation: yeast extract 4-20 g / L; beef extract 8-20 g / L; bacteriological peptone 4-20 g / L; glucose 10-30 g / L; KH2PO4 0.1-0.2 g / L; Na2HPO4.12H2O 0.1-0.5 g / L; MgSO4.7H2O 0.1-1 g / L; FeSO4.7H2O 0.1-1 g / L; CaCl2.2H2O 0.02-0.1 g / L; and pH 7.2+ / -0.2. In the application, the yeast extract, beef extract and bacteriological peptone are mixed as the nitrogen source, which can promote the accumulation of paracoccus biomass and astaxanthin, and meanwhile, the problem of precipitation after sterilization of the culture medium is solved.

The invention relates to the technical field of soil beneficial bacteria, in particular to agricultural soil environment-friendly beneficial bacteria and a preparation method thereof. The preparation method comprises the following steps that S1, a culture medium is prepared, raw materials of the culture medium comprise beef extract, peptone, modified agar, sodium chloride, rice bran and water, and the raw materials are mixed to prepare the culture medium; according to the culture medium disclosed by the invention, after the agar is subjected to heating treatment, compact holes can be formed in a matrix of the agar, and the air permeability and the water permeability of the culture medium can be improved by utilizing a porous structure of the agar, so that more living space is provided for culture of beneficial bacteria, and growth and reproduction of the beneficial bacteria are promoted; according to the present invention, the cell wall can be broken so as to increase the specific surface area, and the fibril structure in the cellulose can be broken after the steam pressure treatment so as to increase the attachment site of the microorganisms, such that the survival rate of the microorganisms in the beneficial bacteria is further improved.

The application discloses a composition for enhancing the immunity of pets and a preparation method thereof, and comprises the following raw materials in parts by weight: 25-40 parts of Echinacea purpurea powder, 5-8 parts of petunidin-3-trans-p-coumaroylerianoside-5-glucoside monomer, 35-55 parts of willow bark extract, and 60-80 parts of a carrier. The composition of the application effectively improves the immunity of pets, increases the weight of pets, and promotes the growth of pets by compounding the Echinacea purpurea powder, the petunidin-3-trans-p-coumaroylerianoside-5-glucoside monomer and the willow bark extract, and synergistic effect. Meanwhile, the beef extract as the carrier also has the effects of supplementing nutrition and enhancing constitution, and can enhance the effect of the composition of the application to a certain extent.

The invention relates to the technical field of food, in particular to beef enzymatic hydrolysate, a beef extract base and a preparation method thereof. According to the method, enzymolysis conditions of beef are explored and optimized through a large number of experiments, beef is subjected to enzymolysis through specific compound protease to obtain beef enzymolysis liquid with the high hydrolysis degree, the beef enzymolysis liquid serves as a raw material, the beef extract base is prepared through the Maillard reaction, and the obtained beef extract base is high in quality, brown and rich in flavor. The beef extract base has rich meat flavor, beef characteristic flavor and roasting flavor, has obvious delicate flavor and fragrant and sweet flavor, can provide richer and more vivid meat flavor for various processed foods, and enhances the eating experience of consumers. The research on the beef extract base not only has important economic value on food industry, but also has far-reaching influence on the aspects of scientific exploration, environmental protection, social development and the like.

The invention belongs to the technical field of biology, and relates to an isolation culture medium and a culture method of lactobacillus acidophilus. The culture medium comprises peptone, beef extract, yeast extract, glucose, anhydrous sodium acetate, tween-80, monopotassium phosphate, dipotassium phosphate, diammonium hydrogen citrate, magnesium sulfate heptahydrate, manganese sulfate heptahydrate, ferrous sulfate heptahydrate, lactic acid, acetic acid, cysteine and fermented grain extract. According to the culture medium formula and the culture method disclosed by the invention, efficient enrichment and separation of lactobacillus acidophilus are realized.

To provide a Western-style seasoning that can easily reproduce bouillon flavor and that has enhanced dashi flavor without using any animal-derived ingredients or animal-derived food materials.SOLUTION: The Western-style seasoning contains one or more selected from the group consisting of beef extract and chicken extract, and malic acid.SELECTED DRAWING: None