33 results about "Beef extract" patented technology

This invention discloses an improved PMA culture medium for Agaricus bisporus mother culture and its application, belonging to the field of edible fungi cultivation technology. The culture medium of this invention comprises PMA medium, Agaricus bisporus substrate residue, and peptone or beef extract. Experiments show that adding Agaricus bisporus substrate residue to the PMA medium significantly promotes mycelial growth. Further addition of a specific amount of peptone or beef extract produces a synergistic effect, increasing the mycelial growth rate to over 6.53 mm / day, and shortening the mother culture cycle from the traditional 23-25 ​​days to approximately 13-15 days. This invention improves the efficiency of spawn production, reduces production costs and pollution risks, and realizes the resource utilization of substrate residue.

The invention relates to the technical field of Escherichia coli detection, and particularly discloses a semisolid culture medium for rapid detection of Escherichia coli and a detection method and application thereof, the semisolid culture medium comprises the following components by weight: 4.2-5.8 g / L of agar; 8.5 to 11.5 g / L of tryptone; the semi-solid culture medium comprises the following components: 3.2 to 4.8 g / L of beef extract, 6.5 to 9.5 g / L of lactose, 1.2 to 2.2 g / L of D-glucose, 4.2 to 5.8 g / L of sodium chloride, 42000 to 58000 [mu] g / L of penicillin, 0.12 to 0.28 g / L of cholate, 0.06 to 0.14 g / L of disodium EDTA (Ethylene Diamine Tetraacetic Acid), 0.12 to 0.22 g / L of L-cysteine, 1.2 to 2.8 g / L of yeast extract, 0.005 to 0.015 g / L of vitamin B and an acid-base indicator, the semisolid culture medium and the detection method thereof provided by the invention not only solve the core pain point of the existing escherichia coli detection method in the technical level, but also reduce the operation cost and improve the benefit for enterprises in the economic level, promote the standardization and high efficiency of the food microorganism detection technology in the industry level, and have practicability and industrial popularization value.

The invention belongs to the technical field of microorganisms, and discloses a high-density fermentation process of vagina-sourced lactobacillus crispatus. The invention discloses a fermentation medium which comprises glucose, tryptone, beef extract, yeast extract, dipotassium phosphate, ammonium citrate, L-cysteine or salt thereof, magnesium sulfate and manganese sulfate. According to the fermentation culture medium provided by the invention, through scientific compounding of a carbon source, a nitrogen source and key factors, the final viable concentration and thallus activity of vagina-derived lactobacillus crispatus can be remarkably improved. The fermentation culture medium is adaptive to the micro-aerobic metabolism characteristics of vagina-derived lactobacillus crispatus by precisely regulating and controlling the dissolved oxygen level (initial dissolved oxygen is controlled to be 5% or below), and when the fermentation culture medium is used for culturing lactobacillus crispatus, the fermentation delay period can be remarkably shortened to 2-3 h, the final viable bacterium yield is greatly increased to 1 * 10 < 10 > CFU / mL or above, and the production cost is reduced. And an efficient and stable technical support is provided for industrial production of the probiotic preparation.

The invention relates to the technical field of decay accelerators, in particular to a composite organism-based soil improvement decay accelerator and a preparation method thereof. The composite organism-based soil improvement decay accelerator is prepared from the following raw materials in parts by weight: 20 to 30 parts of molasses, 5 to 10 parts of eggs, 3 to 8 parts of beef extract, 4 to 12 parts of fresh milk, 6 to 15 parts of crushed trash fish, 2 to 5 parts of urea, 1 to 3 parts of monopotassium phosphate, 0.4 to 0.6 part of fungicide, 1 to 3 parts of tea saponin and 6 to 8 parts of additive. According to the invention, the sodium alginate-vermiculite porous carrier structure of the additive is utilized to realize physical embedding and slow release of the pesticide, the major technical contradiction that viable bacteria and the pesticide are difficult to be compatible is effectively solved, and meanwhile, the carrier integrates the functional components such as humic acid and calcium carbonate, so that the carrier has a good application prospect. The comprehensive effects of accelerating decomposition, preventing and controlling plant diseases and insect pests and improving the soil structure can be synchronously realized after the decomposition accelerant is applied, and the defects that a traditional decomposition accelerant is single in function and needs to be applied for multiple times are overcome.

To provide a meat extract seasoning suppressed in generation of foreign taste and odor, reduced in odor peculiar to livestock, and excellent in flavor, and to provide a method for producing the meat extract seasoning.SOLUTION: The meat extract seasoning contains a raw material meat extract (beef extract, pork extract, chicken extract, etc.) and 0.5 to 5% by weight of a Maillard reaction product derived from 1, 5-D-anhydrofructose obtained by heating a sugar solution containing 1, 5-D-anhydrofructose at a temperature in the range of 60 °C to 150 °C for 30 seconds to 100 hours.SELECTED DRAWING: None

The invention relates to the technical field of environmental engineering, in particular to a photo-thermal evaporation material for salted loess site remediation and a preparation method and application thereof. The preparation method comprises the following steps: dispersing modified zeolite on the surface of a polypropylene non-woven fabric, sequentially spraying a pyrrole aqueous solution and a ferric chloride aqueous solution, continuously and repeatedly coating the modified zeolite, spraying the pyrrole aqueous solution and the ferric chloride aqueous solution, and performing in-situ polymerization to form a cross-linked coating; the preparation method comprises the following steps: preparing a spore suspension from beef extract, peptone, a manganese sulfate titanium dioxide solution, a nickel chloride hexahydrate solution, a yeast extract, an ammonium chloride solution and a sodium chloride solution, sequentially soaking a polypropylene non-woven fabric with a cross-linked coating formed on the surface into the spore suspension and a sodium alginate-graphene oxide mixed solution, spraying a calcium chloride solution, and drying to obtain the polypropylene non-woven fabric with the cross-linked coating formed on the surface. The photo-thermal evaporation material has the advantages that the photo-thermal conversion capability and the light absorption rate are excellent, the moisture evaporation rate is high, and the salt content of the salted loess can be obviously reduced in a short time.

The invention relates to the technical field of porous material preparation, in particular to microorganism-gel synergistic reinforced fly ash ceramsite and a preparation method thereof. The invention relates to a culture medium for culturing bacillus Coriolis, which takes a circulating fluidized bed fly ash leaching solution as a solvent and comprises the following components: 5-10g / L peptone, 3-5g / L beef extract, 0.42-0.5 g / L sodium bicarbonate and 0.53-0.65 g / L sodium carbonate. According to the method, fly ash ceramsite is prepared from the bacillus Coriolis cultured by the culture medium, lightweight non-fired ceramsite with high porosity can be prepared, and a theoretical model and an experimental example are provided for development of a low-carbon, low-energy-consumption and high-added-value solid waste recycling technology.

The application discloses an improved PDA culture medium for Agaricus bisporus mother culture and application and belongs to the technical field of edible mushroom cultivation. The improved PDA culture medium for Agaricus bisporus mother culture is based on a PDA culture medium and is added with Agaricus bisporus residue and peptone or beef extract. The culture medium provided by the application can significantly promote the growth of Agaricus bisporus mycelium, shortens the mother culture time from 23-25 days of the traditional method to about 13-15 days, greatly improves the strain production efficiency, and reduces the production cost and pollution risk.

The utility model relates to the technical field of food processing, in particular to a beef grinder for preparing beef extract, which comprises an upper processing box body, a fixing shell is fixedly connected to the middle of the upper end of the upper processing box body, a driving device is fixedly connected to the middle of the upper end of the fixing shell, and a limiting groove is formed in the middle of the rear end of a lower processing box body. Supporting legs are fixedly connected to the four corners of the lower end of the lower treatment box body, lower plates are fixedly connected to the left portion and the right portion of the lower end of the lower treatment box body, and extrusion devices are fixedly connected between the left ends and the right ends of the two lower plates. According to the beef crusher for preparing the beef extract, the driving device and the crushing assembly are cooperated, the crushing efficiency and the granularity uniformity are improved through the inclined crushing cutter and the blade-replaceable crushing assembly, meanwhile, the problem that blades of existing equipment are prone to abrasion is solved, and the durability of the blades and the adaptability of the equipment to different beef textures are enhanced.

This invention provides a direct-inoculation lactic acid bacteria starter culture, its preparation method, and its application. The method includes: inoculating activated lactic acid bacteria strains into a nucleotide medium for expansion culture to obtain a high-density lactic acid bacteria inoculum; centrifuging the high-density lactic acid bacteria inoculum, mixing the bacterial cells with a freeze-drying protectant, and freeze-drying to obtain the direct-inoculation lactic acid bacteria starter culture; wherein the nucleotide medium comprises: 0.3–0.8 g / L nucleotides, 2–10 g / L beef extract powder, 25–35 g / L soybean peptone, 10–17 g / L sucrose, 1–6 g / L anhydrous sodium acetate, 2–8 g / L triammonium citrate, 1.0–1.6 g / L potassium dihydrogen phosphate, 0.1–0.8 g / L magnesium sulfate monohydrate, 0.023–0.029 g / L manganese sulfate, and 2–6 g / L Tween 80; the nucleotides include at least one selected from guanine, adenine, xanthine, or uracil. The method provided by this invention can improve the activity and stress resistance of lactic acid bacteria strains, and enhance their adaptability to harsh environments, thereby obtaining a direct-inoculation lactic acid bacteria starter with high density and freeze-drying survival rate.

The invention discloses a culture medium for promoting intestinal colonization of probiotics. Every 1000 parts by weight of water of the culture medium is prepared from the following components in parts by weight: 2 to 10 parts of casein, 4 to 12 parts of yeast extract powder, 10 to 12 parts of beef extract powder, 1.5 to 2.5 parts of trisodium citrate or triammonium citrate, 4.5 to 5.5 parts of sodium acetate, 0.1 to 0.3 part of magnesium sulfate heptahydrate, 0.05 to 0.1 part of manganese chloride and 1 to 3 parts of dipotassium phosphate. When the culture medium is used for culturing the probiotics, the secretion amount of exopolysaccharides, proteins and other substances can be remarkably increased, a protective layer is formed, the stress resistance of the strain is improved, the survival ability of the probiotics in a complex gastrointestinal tract environment is improved, and a new strategy is provided for development of the oral probiotics.

The grinding tool for preparing the flavor type beef extract comprises a grinding barrel, the top of the grinding barrel is movably connected with a feeding cover through a rotating shaft, a grinding mechanism is arranged in the grinding barrel, and the grinding mechanism comprises a grinding disc, a grinding motor, a protective cover and an extension rod. The bottom of the extension rod is fixedly connected to the top of the protective cover, the grinding motor is fixedly connected to the interior of the protective cover, the top of the grinding disc is fixedly connected to the output end of the grinding motor, a separation net is fixedly connected to the interior of the grinding barrel, and the radian of the separation net is the same as that of the grinding disc. Through the grinding mechanism, when internal raw materials are ground, a telescopic air cylinder is started, the telescopic air cylinder pushes a protection plate to move downwards, a grinding disc makes contact with the raw materials, the raw materials are ground through rotation of the grinding disc, and the raw materials are fully ground; and by arranging the separation net, the qualified ground raw materials enter the bottom of the grinding barrel through the separation net.

The invention discloses a method for removing an organic pollutant bisphenol A, and belongs to the technical field of water treatment. The removal method comprises the following steps: (1) inoculating pseudomonas chlororaphis into a culture medium taking waste molasses as a main raw material, carrying out shake culture at 34-36 DEG C, culturing for at least 24 hours by using the culture medium comprising 1.3-1.4 g / L of waste molasses, 2-4 g / L of beef extract and 4-6 g / L of sodium chloride, centrifuging, and taking supernatant to obtain a culture solution; and (2) adding the culture solution in the step (1) into an anode chamber of a microbial fuel cell reactor, diluting with wastewater, and adjusting the COD content to 1950-2050mg / L. According to the invention, the degradation efficiency and the electricity generation performance of the system on bisphenol A can be obviously enhanced.

The object of the present invention is to provide a flavor-enhancing composition that can enhance the flavor of food and beverages with a small amount of addition, and more specifically, to provide a composition that can efficiently enhance the savory flavor of meat, the flavor of stewed dishes, and other cooked flavors and richness. [Solution] The present inventors have conducted various studies to solve the above problem and have found that the above problem can be solved by preparing a composition obtained by heating a mixture of (a) beef extract, (b) glucose, and (c) fructose that satisfies "a:(b+c)=1:1.2~5", "b:c=1:0~4", and "60% by weight ≤ a+b+c ≤ 77% by weight" (where a, b, and c are all solid content equivalent values).

This invention belongs to the field of turfgrass and forage grass maintenance technology, specifically relating to a microbial selenium protein preparation for alleviating drought stress in perennial ryegrass, its preparation method, application method, and application. The microbial selenium protein preparation comprises the following raw materials in specific weight proportions: peptone, beef extract, sucrose, salt, selenium-enriched mushroom powder, organosilicon, calcium amino acids, potassium humate, betaine, salicylic acid, and Bacillus natto. This microbial selenium protein preparation aims to solve the limitations of traditional drought-resistant methods and the poor effectiveness of single-component preparations. When using this microbial selenium protein preparation, it is first diluted and then applied as a root drench to ryegrass seedlings multiple times. The selenium protein preparation of this invention, through the synergistic effect of multiple components, can significantly improve the drought resistance of ryegrass. Furthermore, the preparation process of this protein preparation is simple, environmentally friendly, and efficient, suitable for ryegrass propagation and maintenance, and has broad application prospects.

The invention relates to the technical field of pathogenic bacteria culture and preservation. The invention provides a culture medium and a preservation solution for pathogenic bacteria of soft rot of konjak and a use method of the culture medium and the preservation solution. The culture medium is prepared from the following raw materials in parts by weight: 2.5-3.5 parts of beef extract, 9-11 parts of peptone, 4.5-5.5 parts of sodium chloride, 4-6 parts of agar, 4-6 parts of whole konjak powder and 950-1050 parts of water. According to the invention, a konjac soft rot pathogen culture medium formula and a preservation method are improved by utilizing an interaction relationship between konjac soft rot pathogens and konjac. The amorphophallus konjac whole powder is used for replacing part of agar powder and other nutrient substances, the soft rot pathogenic bacteria can be efficiently cultured, and meanwhile, the preservation time of the strain can be remarkably prolonged in cooperation with a specific preservation solution and a preservation method.

The application discloses a culture medium for improving production of paracoccus astaxanthin and application thereof. The culture medium comprises the following components according to concentration calculation: yeast extract 4-20 g / L; beef extract 8-20 g / L; bacteriological peptone 4-20 g / L; glucose 10-30 g / L; KH2PO4 0.1-0.2 g / L; Na2HPO4.12H2O 0.1-0.5 g / L; MgSO4.7H2O 0.1-1 g / L; FeSO4.7H2O 0.1-1 g / L; CaCl2.2H2O 0.02-0.1 g / L; and pH 7.2+ / -0.2. In the application, the yeast extract, beef extract and bacteriological peptone are mixed as the nitrogen source, which can promote the accumulation of paracoccus biomass and astaxanthin, and meanwhile, the problem of precipitation after sterilization of the culture medium is solved.

The application discloses a composition for enhancing the immunity of pets and a preparation method thereof, and comprises the following raw materials in parts by weight: 25-40 parts of Echinacea purpurea powder, 5-8 parts of petunidin-3-trans-p-coumaroylerianoside-5-glucoside monomer, 35-55 parts of willow bark extract, and 60-80 parts of a carrier. The composition of the application effectively improves the immunity of pets, increases the weight of pets, and promotes the growth of pets by compounding the Echinacea purpurea powder, the petunidin-3-trans-p-coumaroylerianoside-5-glucoside monomer and the willow bark extract, and synergistic effect. Meanwhile, the beef extract as the carrier also has the effects of supplementing nutrition and enhancing constitution, and can enhance the effect of the composition of the application to a certain extent.

The invention belongs to the technical field of biology, and relates to an isolation culture medium and a culture method of lactobacillus acidophilus. The culture medium comprises peptone, beef extract, yeast extract, glucose, anhydrous sodium acetate, tween-80, monopotassium phosphate, dipotassium phosphate, diammonium hydrogen citrate, magnesium sulfate heptahydrate, manganese sulfate heptahydrate, ferrous sulfate heptahydrate, lactic acid, acetic acid, cysteine and fermented grain extract. According to the culture medium formula and the culture method disclosed by the invention, efficient enrichment and separation of lactobacillus acidophilus are realized.

To provide a Western-style seasoning that can easily reproduce bouillon flavor and that has enhanced dashi flavor without using any animal-derived ingredients or animal-derived food materials.SOLUTION: The Western-style seasoning contains one or more selected from the group consisting of beef extract and chicken extract, and malic acid.SELECTED DRAWING: None

The application belongs to the technical field of microbial fermentation, and particularly relates to a high-density fermentation metabolic regulation method of lactic acid bacteria. The application provides a high-density culture method of lactic acid bacteria. The formula of the high-density fermentation culture medium is as follows: 10-20 g / L of proteose peptone, 10-15 g / L of beef extract, 5-10 g / L of yeast powder, 8-15 g / L of D-sorbitol, 5-10 g / L of L-proline, 3-7 g / L of sodium chloride, 0.3-0.6 g / L of magnesium sulfate 7H2O, 0.1-0.2 g / L of manganese sulfate 1H2O, 0.1-0.2 g / L of ferrous sulfate 7H2O, and pH is 6.0-7.0. The fermentation culture medium significantly improves the viable cell count of Lactobacillus fermentum CQPC04, Lactobacillus rhamnosus 4F225, Lactobacillus plantarum H6 and Lactobacillus gasseri GS18, realizes high-density culture of lactic acid bacteria, and lays a foundation for industrialized production of lactic acid bacteria.

The invention relates to the technical field of microorganisms, and discloses a VBNC state staphylococcus aureus induction method, a resuscitation medium and application, the resuscitation medium comprises Rpf recombinant protein, MgSO4, peptone, beef extract and sodium chloride, and the mass concentration ratio of the Rpf recombinant protein to the MgSO4 to the peptone to the beef extract to the sodium chloride is (90 ng / L to 110 ng / L): (0.5 g / L to 0.7 g / L): (9 g / L to 11 g / L): (4 g / L to 6 g / L): (70 g / L to 80 g / L); the nucleotide sequence of the Rpf recombinant protein is as shown in SEQ ID NO. 1. The resuscitation medium provided by the invention can successfully resuscitate staphylococcus aureus in a VBNC state.

The invention belongs to the technical field of microbial fermentation, discloses a liquid fermentation optimization process of a biocontrol compound bacterium system for effectively inhibiting ganoderma lucidum mycorrhizal rot, and aims to optimize a biocontrol compound bacterium system fermentation culture medium and fermentation conditions constructed by pseudomonas aeruginosa and bacillus licheniformis by taking the size of a plate antagonistic ganoderma lucidum bacterium inhibition zone as a target. The optimized culture medium comprises the following main components: 3% of cane sugar, 0.5% of ammonium sulfate, 1% of beef extract and 2% of potassium chloride; the optimal fermentation conditions are as follows: the temperature is 32 DEG C, the initial pH value is 7, the inoculum size is 3%, the time is 24 hours, and the rotating speed is 220 rpm. The inhibition rate of the optimized compound bacterium system on ganoderma lucidum is increased to 88.7%. According to the method for optimizing the fermentation culture medium and the fermentation conditions of the compound bacterium system, the biocontrol capability can be improved, the culture time can be shortened, the cost can be saved, and a theoretical basis is provided for subsequent development and application of a biocontrol bacterium agent for preventing and treating the delonix regia root rot.

The invention discloses a lactobacillus plantarum high-density fermentation medium and a fermentation method, and belongs to the field of microbial fermention.The high-density fermentation medium comprises 1-2% of peptone, 1-2% of glucose, 1-2% of beef extract, self-made yeast powder, Tween, sodium acetate, diammonium hydrogen citrate, dipotassium phosphate and the like. According to the method, a lactobacillus plantarum seed solution is inoculated into a high-density fermentation culture medium for fermentation, in the fermentation process, the fermentation temperature is kept at 37 DEG C, the pH value is controlled to be 6.5-7.0 through 10% ammonia water, the dissolved oxygen content is kept to be 6-9 mg / L, a continuous fed-batch feeding mode is adopted, and the feeding speed is regulated in real time according to the biomass and nutrient substance concentration of fermentation liquor; and carrying out high-density fermentation with the total fermentation time of 12 hours. According to the method, high-density fermentation of lactobacillus plantarum is realized, the production cost is reduced, and the production efficiency is improved.

The invention relates to a high-density fermentation method of lactobacillus mucus fermentation, lactobacillus mucus fermentation freeze-dried powder and a preparation method, and belongs to the technical field of microbial fermentation, and the method comprises the following steps: inoculating a bouillon culture medium with a lactobacillus mucus fermentation MSJK0025 strain for primary activation; inoculating the primary activation solution into an optimized culture medium for secondary propagation, wherein the optimized culture medium contains yeast peptone FP103, a beef extract, yeast extract powder FM902, glucose, dipotassium phosphate, citric acid monohydrate, sodium acetate, magnesium sulfate, manganese sulfate and Tween 80; and inoculating the second-stage expanding culture solution into the optimized culture medium, and introducing nitrogen into a fermentation tank with the pressure of 0.01-0.04 MPa to carry out third-stage fermentation, so as to obtain the fermented lactobacillus mucus MSJK0025 culture solution. By adopting the fermentation method disclosed by the invention, the thallus yield of the fermented lactobacillus mucus MSJK0025 can be improved, and the fermentation period is shortened.

The application discloses a method for preparing high-content shikimic acid bacterial substance, which comprises the following steps: adding one or more of rice bean powder, yam powder, peptone and beef extract into star anise powder and ginkgo leaf powder, and adding pure water, sterilizing to prepare a solid culture medium; inoculating activated cordyceps liquid bacterial seed into the solid culture medium, and culturing at 15-35 DEG C in dark to obtain the solid bacterial substance with increased shikimic acid content; and the method uses star anise, ginkgo leaf and other raw medicinal materials as fermentation substrates, generates bacterial substance through one-time fermentation, and the shikimic acid content in the bacterial substance is obviously increased compared with blank control medium, so that a new way is provided for deep development and utilization of star anise, ginkgo leaf and other traditional Chinese medicine resources.

This invention discloses a high-yield 1-deoxynojirimycin-producing *Bacillus pumilus* strain and its applications, belonging to the field of microbiology. This invention, through screening, ARTP and NTG mutagenesis, obtained a *Bacillus pumilus* strain with significantly increased 1-deoxynojirimycin production. Bacillus pumilus DNJ-BP01, deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 38218, was used to inoculate the seed culture of Bacillus pumilus DNJ-BP01 into a fermentation medium containing 5% beef extract supplemented with 2.5% mannitol as the carbon source and 3% potassium nitrate as the nitrogen source. After 76 hours of aerobic fermentation, the yield of 1-deoxynojirimycin reached 0.892 g / L. This invention provides a new fermentation strain for the efficient industrial production of 1-deoxynojirimycin and has broad application prospects.

The invention relates to the technical field of microbial culture, in particular to an engineering bifidobacterium product production method based on an in-vitro continuous fermentation method, which comprises the following steps: S1, strain breeding and preparation; s2, culture medium design and sterilization treatment; s3, fermentation process control; and S4, separating and purifying the product. According to the present invention, the material supplementing strategy is optimized, and the material supplementing rate of the material supplementing liquid added to the three-stage fermentation tank is regulated and controlled according to the specific growth rate of the current thalli in the three-stage fermentation tank and the exponential change of the thalli in the logarithmic phase during the fermentation culture so as to effectively and completely supply the carbon source and the nitrogen source, ensure the rapid growth of the thalli in the fermentation tank, and improve the fermentation efficiency. Meanwhile, the ratio of glucose to the beef extract in the feed supplementing liquid is dynamically adjusted, so that the carbon nitrogen ratio R in the three-stage fermentation tank is kept stable, the continuous fermentation process can be efficiently operated for a long time, and the number and scale of thalli after fermentation are improved.

The invention discloses a culture medium and a culture method for culturing human intestinal flora in vitro, every 1000g of the culture medium contains 5-20g of casein tryptone, 1-10g of beef extract, 1-10g of soybean oligosaccharide, 0.1-1g of monopotassium phosphate, 1-15g of sodium chloride, 0.5-5mg of hemin, 1-5g of cysteine hydrochloric acid, 0.5-2g of cholate, 20-100g of microcrystalline cellulose and 1-5g of agar powder, water is added to fix the volume to 1000mL, and the pH value is adjusted to 6.0-7.5. And after high-temperature steam sterilization, cooling to 40 DEG C, adding 0.5-5 / 1000 (V / V) serum, and uniformly mixing. The invention provides a culture medium and a culture method capable of culturing flora growth in human excrement twice through passage. In-vitro culture similar to intestinal flora characteristics of healthy donors is successfully realized, so that an ideal intestinal bacteria product is provided for intestinal bacteria transplantation treatment.