19 results about "Beef extract" patented technology

This invention discloses an improved PMA culture medium for Agaricus bisporus mother culture and its application, belonging to the field of edible fungi cultivation technology. The culture medium of this invention comprises PMA medium, Agaricus bisporus substrate residue, and peptone or beef extract. Experiments show that adding Agaricus bisporus substrate residue to the PMA medium significantly promotes mycelial growth. Further addition of a specific amount of peptone or beef extract produces a synergistic effect, increasing the mycelial growth rate to over 6.53 mm / day, and shortening the mother culture cycle from the traditional 23-25 ​​days to approximately 13-15 days. This invention improves the efficiency of spawn production, reduces production costs and pollution risks, and realizes the resource utilization of substrate residue.

The invention belongs to the technical field of microorganisms, and discloses a high-density fermentation process of vagina-sourced lactobacillus crispatus. The invention discloses a fermentation medium which comprises glucose, tryptone, beef extract, yeast extract, dipotassium phosphate, ammonium citrate, L-cysteine or salt thereof, magnesium sulfate and manganese sulfate. According to the fermentation culture medium provided by the invention, through scientific compounding of a carbon source, a nitrogen source and key factors, the final viable concentration and thallus activity of vagina-derived lactobacillus crispatus can be remarkably improved. The fermentation culture medium is adaptive to the micro-aerobic metabolism characteristics of vagina-derived lactobacillus crispatus by precisely regulating and controlling the dissolved oxygen level (initial dissolved oxygen is controlled to be 5% or below), and when the fermentation culture medium is used for culturing lactobacillus crispatus, the fermentation delay period can be remarkably shortened to 2-3 h, the final viable bacterium yield is greatly increased to 1 * 10 < 10 > CFU / mL or above, and the production cost is reduced. And an efficient and stable technical support is provided for industrial production of the probiotic preparation.

The invention relates to the technical field of decay accelerators, in particular to a composite organism-based soil improvement decay accelerator and a preparation method thereof. The composite organism-based soil improvement decay accelerator is prepared from the following raw materials in parts by weight: 20 to 30 parts of molasses, 5 to 10 parts of eggs, 3 to 8 parts of beef extract, 4 to 12 parts of fresh milk, 6 to 15 parts of crushed trash fish, 2 to 5 parts of urea, 1 to 3 parts of monopotassium phosphate, 0.4 to 0.6 part of fungicide, 1 to 3 parts of tea saponin and 6 to 8 parts of additive. According to the invention, the sodium alginate-vermiculite porous carrier structure of the additive is utilized to realize physical embedding and slow release of the pesticide, the major technical contradiction that viable bacteria and the pesticide are difficult to be compatible is effectively solved, and meanwhile, the carrier integrates the functional components such as humic acid and calcium carbonate, so that the carrier has a good application prospect. The comprehensive effects of accelerating decomposition, preventing and controlling plant diseases and insect pests and improving the soil structure can be synchronously realized after the decomposition accelerant is applied, and the defects that a traditional decomposition accelerant is single in function and needs to be applied for multiple times are overcome.

To provide a meat extract seasoning suppressed in generation of foreign taste and odor, reduced in odor peculiar to livestock, and excellent in flavor, and to provide a method for producing the meat extract seasoning.SOLUTION: The meat extract seasoning contains a raw material meat extract (beef extract, pork extract, chicken extract, etc.) and 0.5 to 5% by weight of a Maillard reaction product derived from 1, 5-D-anhydrofructose obtained by heating a sugar solution containing 1, 5-D-anhydrofructose at a temperature in the range of 60 °C to 150 °C for 30 seconds to 100 hours.SELECTED DRAWING: None

The invention relates to the technical field of environmental engineering, in particular to a photo-thermal evaporation material for salted loess site remediation and a preparation method and application thereof. The preparation method comprises the following steps: dispersing modified zeolite on the surface of a polypropylene non-woven fabric, sequentially spraying a pyrrole aqueous solution and a ferric chloride aqueous solution, continuously and repeatedly coating the modified zeolite, spraying the pyrrole aqueous solution and the ferric chloride aqueous solution, and performing in-situ polymerization to form a cross-linked coating; the preparation method comprises the following steps: preparing a spore suspension from beef extract, peptone, a manganese sulfate titanium dioxide solution, a nickel chloride hexahydrate solution, a yeast extract, an ammonium chloride solution and a sodium chloride solution, sequentially soaking a polypropylene non-woven fabric with a cross-linked coating formed on the surface into the spore suspension and a sodium alginate-graphene oxide mixed solution, spraying a calcium chloride solution, and drying to obtain the polypropylene non-woven fabric with the cross-linked coating formed on the surface. The photo-thermal evaporation material has the advantages that the photo-thermal conversion capability and the light absorption rate are excellent, the moisture evaporation rate is high, and the salt content of the salted loess can be obviously reduced in a short time.

The invention relates to the technical field of porous material preparation, in particular to microorganism-gel synergistic reinforced fly ash ceramsite and a preparation method thereof. The invention relates to a culture medium for culturing bacillus Coriolis, which takes a circulating fluidized bed fly ash leaching solution as a solvent and comprises the following components: 5-10g / L peptone, 3-5g / L beef extract, 0.42-0.5 g / L sodium bicarbonate and 0.53-0.65 g / L sodium carbonate. According to the method, fly ash ceramsite is prepared from the bacillus Coriolis cultured by the culture medium, lightweight non-fired ceramsite with high porosity can be prepared, and a theoretical model and an experimental example are provided for development of a low-carbon, low-energy-consumption and high-added-value solid waste recycling technology.

The application discloses an improved PDA culture medium for Agaricus bisporus mother culture and application and belongs to the technical field of edible mushroom cultivation. The improved PDA culture medium for Agaricus bisporus mother culture is based on a PDA culture medium and is added with Agaricus bisporus residue and peptone or beef extract. The culture medium provided by the application can significantly promote the growth of Agaricus bisporus mycelium, shortens the mother culture time from 23-25 days of the traditional method to about 13-15 days, greatly improves the strain production efficiency, and reduces the production cost and pollution risk.

The utility model relates to the technical field of food processing, in particular to a beef grinder for preparing beef extract, which comprises an upper processing box body, a fixing shell is fixedly connected to the middle of the upper end of the upper processing box body, a driving device is fixedly connected to the middle of the upper end of the fixing shell, and a limiting groove is formed in the middle of the rear end of a lower processing box body. Supporting legs are fixedly connected to the four corners of the lower end of the lower treatment box body, lower plates are fixedly connected to the left portion and the right portion of the lower end of the lower treatment box body, and extrusion devices are fixedly connected between the left ends and the right ends of the two lower plates. According to the beef crusher for preparing the beef extract, the driving device and the crushing assembly are cooperated, the crushing efficiency and the granularity uniformity are improved through the inclined crushing cutter and the blade-replaceable crushing assembly, meanwhile, the problem that blades of existing equipment are prone to abrasion is solved, and the durability of the blades and the adaptability of the equipment to different beef textures are enhanced.

The grinding tool for preparing the flavor type beef extract comprises a grinding barrel, the top of the grinding barrel is movably connected with a feeding cover through a rotating shaft, a grinding mechanism is arranged in the grinding barrel, and the grinding mechanism comprises a grinding disc, a grinding motor, a protective cover and an extension rod. The bottom of the extension rod is fixedly connected to the top of the protective cover, the grinding motor is fixedly connected to the interior of the protective cover, the top of the grinding disc is fixedly connected to the output end of the grinding motor, a separation net is fixedly connected to the interior of the grinding barrel, and the radian of the separation net is the same as that of the grinding disc. Through the grinding mechanism, when internal raw materials are ground, a telescopic air cylinder is started, the telescopic air cylinder pushes a protection plate to move downwards, a grinding disc makes contact with the raw materials, the raw materials are ground through rotation of the grinding disc, and the raw materials are fully ground; and by arranging the separation net, the qualified ground raw materials enter the bottom of the grinding barrel through the separation net.

The invention discloses a method for removing an organic pollutant bisphenol A, and belongs to the technical field of water treatment. The removal method comprises the following steps: (1) inoculating pseudomonas chlororaphis into a culture medium taking waste molasses as a main raw material, carrying out shake culture at 34-36 DEG C, culturing for at least 24 hours by using the culture medium comprising 1.3-1.4 g / L of waste molasses, 2-4 g / L of beef extract and 4-6 g / L of sodium chloride, centrifuging, and taking supernatant to obtain a culture solution; and (2) adding the culture solution in the step (1) into an anode chamber of a microbial fuel cell reactor, diluting with wastewater, and adjusting the COD content to 1950-2050mg / L. According to the invention, the degradation efficiency and the electricity generation performance of the system on bisphenol A can be obviously enhanced.

The object of the present invention is to provide a flavor-enhancing composition that can enhance the flavor of food and beverages with a small amount of addition, and more specifically, to provide a composition that can efficiently enhance the savory flavor of meat, the flavor of stewed dishes, and other cooked flavors and richness. [Solution] The present inventors have conducted various studies to solve the above problem and have found that the above problem can be solved by preparing a composition obtained by heating a mixture of (a) beef extract, (b) glucose, and (c) fructose that satisfies "a:(b+c)=1:1.2~5", "b:c=1:0~4", and "60% by weight ≤ a+b+c ≤ 77% by weight" (where a, b, and c are all solid content equivalent values).

This invention belongs to the field of turfgrass and forage grass maintenance technology, specifically relating to a microbial selenium protein preparation for alleviating drought stress in perennial ryegrass, its preparation method, application method, and application. The microbial selenium protein preparation comprises the following raw materials in specific weight proportions: peptone, beef extract, sucrose, salt, selenium-enriched mushroom powder, organosilicon, calcium amino acids, potassium humate, betaine, salicylic acid, and Bacillus natto. This microbial selenium protein preparation aims to solve the limitations of traditional drought-resistant methods and the poor effectiveness of single-component preparations. When using this microbial selenium protein preparation, it is first diluted and then applied as a root drench to ryegrass seedlings multiple times. The selenium protein preparation of this invention, through the synergistic effect of multiple components, can significantly improve the drought resistance of ryegrass. Furthermore, the preparation process of this protein preparation is simple, environmentally friendly, and efficient, suitable for ryegrass propagation and maintenance, and has broad application prospects.

The application discloses a composition for enhancing the immunity of pets and a preparation method thereof, and comprises the following raw materials in parts by weight: 25-40 parts of Echinacea purpurea powder, 5-8 parts of petunidin-3-trans-p-coumaroylerianoside-5-glucoside monomer, 35-55 parts of willow bark extract, and 60-80 parts of a carrier. The composition of the application effectively improves the immunity of pets, increases the weight of pets, and promotes the growth of pets by compounding the Echinacea purpurea powder, the petunidin-3-trans-p-coumaroylerianoside-5-glucoside monomer and the willow bark extract, and synergistic effect. Meanwhile, the beef extract as the carrier also has the effects of supplementing nutrition and enhancing constitution, and can enhance the effect of the composition of the application to a certain extent.

The application belongs to the technical field of microbial fermentation, and particularly relates to a high-density fermentation metabolic regulation method of lactic acid bacteria. The application provides a high-density culture method of lactic acid bacteria. The formula of the high-density fermentation culture medium is as follows: 10-20 g / L of proteose peptone, 10-15 g / L of beef extract, 5-10 g / L of yeast powder, 8-15 g / L of D-sorbitol, 5-10 g / L of L-proline, 3-7 g / L of sodium chloride, 0.3-0.6 g / L of magnesium sulfate 7H2O, 0.1-0.2 g / L of manganese sulfate 1H2O, 0.1-0.2 g / L of ferrous sulfate 7H2O, and pH is 6.0-7.0. The fermentation culture medium significantly improves the viable cell count of Lactobacillus fermentum CQPC04, Lactobacillus rhamnosus 4F225, Lactobacillus plantarum H6 and Lactobacillus gasseri GS18, realizes high-density culture of lactic acid bacteria, and lays a foundation for industrialized production of lactic acid bacteria.

The invention relates to the technical field of microorganisms, and discloses a VBNC state staphylococcus aureus induction method, a resuscitation medium and application, the resuscitation medium comprises Rpf recombinant protein, MgSO4, peptone, beef extract and sodium chloride, and the mass concentration ratio of the Rpf recombinant protein to the MgSO4 to the peptone to the beef extract to the sodium chloride is (90 ng / L to 110 ng / L): (0.5 g / L to 0.7 g / L): (9 g / L to 11 g / L): (4 g / L to 6 g / L): (70 g / L to 80 g / L); the nucleotide sequence of the Rpf recombinant protein is as shown in SEQ ID NO. 1. The resuscitation medium provided by the invention can successfully resuscitate staphylococcus aureus in a VBNC state.

The invention relates to a high-density fermentation method of lactobacillus mucus fermentation, lactobacillus mucus fermentation freeze-dried powder and a preparation method, and belongs to the technical field of microbial fermentation, and the method comprises the following steps: inoculating a bouillon culture medium with a lactobacillus mucus fermentation MSJK0025 strain for primary activation; inoculating the primary activation solution into an optimized culture medium for secondary propagation, wherein the optimized culture medium contains yeast peptone FP103, a beef extract, yeast extract powder FM902, glucose, dipotassium phosphate, citric acid monohydrate, sodium acetate, magnesium sulfate, manganese sulfate and Tween 80; and inoculating the second-stage expanding culture solution into the optimized culture medium, and introducing nitrogen into a fermentation tank with the pressure of 0.01-0.04 MPa to carry out third-stage fermentation, so as to obtain the fermented lactobacillus mucus MSJK0025 culture solution. By adopting the fermentation method disclosed by the invention, the thallus yield of the fermented lactobacillus mucus MSJK0025 can be improved, and the fermentation period is shortened.

This invention discloses a high-yield 1-deoxynojirimycin-producing *Bacillus pumilus* strain and its applications, belonging to the field of microbiology. This invention, through screening, ARTP and NTG mutagenesis, obtained a *Bacillus pumilus* strain with significantly increased 1-deoxynojirimycin production. Bacillus pumilus DNJ-BP01, deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 38218, was used to inoculate the seed culture of Bacillus pumilus DNJ-BP01 into a fermentation medium containing 5% beef extract supplemented with 2.5% mannitol as the carbon source and 3% potassium nitrate as the nitrogen source. After 76 hours of aerobic fermentation, the yield of 1-deoxynojirimycin reached 0.892 g / L. This invention provides a new fermentation strain for the efficient industrial production of 1-deoxynojirimycin and has broad application prospects.

The application relates to the technical field of environmental engineering, in particular to a light-heat evaporation material for saline loess site remediation and a preparation method and application thereof. The preparation method comprises the following steps: dispersing modified zeolite on the surface of polypropylene non-woven fabric, spraying pyrrole aqueous solution and ferric chloride aqueous solution in sequence, continuously spraying the modified zeolite for multiple times and spraying the pyrrole aqueous solution and the ferric chloride aqueous solution, and in-situ polymerization to form a cross-linked coating; preparing a spore suspension by using beef extract, protein peptone, manganese sulfate titanium white powder solution, nickel chloride hexahydrate solution, yeast extract, ammonium chloride solution and sodium chloride solution; immersing the polypropylene non-woven fabric with the cross-linked coating formed on the surface into the spore suspension and a sodium alginate-graphene oxide mixed solution in sequence; spraying calcium chloride solution; and after rinsing with deionized water and drying, the light-heat evaporation material is obtained. The material has excellent light-heat conversion capacity, light absorption rate and high water evaporation rate, and can significantly reduce the salt content of saline loess in a short time.

The invention relates to the technical field of microbial fermentation, and particularly discloses a pediococcus pentosaceus high-density fermentation medium and a fermentation process. The invention relates to a pediococcus pentosaceus high-density fermentation medium which comprises the following components in mass concentration: a carbon source, a nitrogen source, beef extract powder, yeast extract powder, dipotassium phosphate, ammonium hydrogen citrate, sodium acetate, magnesium sulfate, manganese sulfate, tween-80, L-cysteine hydrochloride and growth factors. The growth factor is selected from a celery juice extract, a potato juice extract, a green pepper juice extract or a tomato juice extract. The pediococcus pentosaceus high-density fermentation culture medium can be used for high-density fermentation of pediococcus pentosaceus, the yield and viable bacterium yield of pediococcus pentosaceus can be remarkably increased, and the industrial production scale and application of pediococcus pentosaceus are greatly expanded.