951 results about "Agaricus bisporus" patented technology

The invention relates to Agrobacterium mediated transformation of moulds comprising species of the fungal sub-divisions Ascomycotina, Basidiomycotina, Deuteromycotina, Mastigomycotina, and Zygomycotina.Examples demonstrate the transformation of Aspergillus awamori (both protoplasts and conidia), Aspergillus nidulans, Aspergillus niger, Colletotrichum gloeosporioides, Fusarium solani pisi, Neurospora crassa, Trichoderma reesei, Pleurotus ostreatus and Agaricus bisporus (all conidia), and Fusarium graminearum (both conidia and rehydrated freeze dried ATCC material).Especially for Aspergillus awamori the transformation frequency is much higher than with conventional mould transformation techniques.It has further been found that not only one expressable gene can be introduced into these moulds, but even multiple copies of such gene, which, moreover, can be targeted e.g. in the chromosomal pyrG locus, as exemplified for A. awamori. These multiple copies can be of a gene encoding a desired, homologous or heterologous, protein.

The present invention relates to bispore mushroom cultivating technology, and is especially one kind of bispore mushroom cultivating medium and its application. The cultivating medium consists of grass material 45í½55 wt%, dung material 35í½45 wt%, cake fertilizer 1í½8 wt%, urine 0.5í½1.0 wt%, gypsum powder 0.5í½1.5 wt%, calcium superphosphate 0.8í½1.5 wt%, lime powder 0.2í½1.5 wt%, calcium carbonate 0í½2.0 wt% and dichlorovos 0í½0.5 wt%. The grass material including rice straw, wheat straw, cotton seed husk, corn cob and corn stalk, and the dung material including cow dung, house dung and chicken dung account for at least 90 wt%. The cake fertilizer may be soybean cake, soybean powder, cotton seed cake and/or corn powder. The cultivating medium is used for cultivating bispore mushroom in tall stalk crop field and has biological conversion efficiency over 60 % and bispore mushroom yield over 25 kg/sq m.

The invention relates to a compound mushroom flavoring and a preparation method thereof. The main raw materials of the compound mushroom flavoring comprise meadow mushroom powder, straw mushroom powder, sodium glutamate, salt, sugar, maltodextrin, yeast extract, disodium 5'-ribonucleotide and yolk powder. The preparation method of the compound mushroom flavoring comprises the following steps: rinsing, boiling, pulverizing, pulping, homogenizing, concentrating and spray-drying fresh mushrooms, and then, obtaining mushroom powder; adding other raw materials; and carrying out the processes of mixing, granulating, drying and the like to obtain the compound mushroom flavoring. The compound mushroom flavoring has the flavor of the meadow mushroom and the straw mushroom simultaneously by organically mixing the sodium glutamate, the yeast extract, the disodium 5'-ribonucleotide and the mushroom powder, has strong functions of increasing freshness and increasing fragrance and has soft flavor. The cell walls of mushrooms can be broken by the homogenizing action to better release the substances in the cells of the mushrooms, thereby further improving the nutrient value of the product. The sugar is added in the concentration process, thereby further improving and increasing the flavor of the materials. The compound mushroom flavoring is granular and is convenient in use.

A melanin preparation as an immunostimulatory agent from at least one of the following botanicals: Echinacea, American ginseng, black walnut, green tea, Parthenium integrifolium, Korean ginseng, alfalfa sprouts, ginger, goldenseal, red clover, dandelion, black cohosh, licorice, chamomile, milk thistle, alfalfa, horsetail, astragalus, gotu kola, feverfew, valerian, hawthorn, rosemary, saw palmetto, ephedra, pau d'arco, ginkgo, garlic, St. John's wort, Agaricus bisporus (common mushroom), Agaricus bisporus brown strain (portabella mushroom), Lentinus edodes (shiitake mushroom) or Boletus edulis (porcini mushroom). Also disclosed is methods of treating a subject requiring immune mediation comprising administering to said subject a therapeutically effective amount of a melanin preparation from any one of the following botanicals: Echinacea, American ginseng, black walnut, green tea, Parthenium integrifolium, Korean ginseng, alfalfa sprouts, ginger, goldenseal, red clover, dandelion, black cohosh, licorice, chamomile, milk thistle, alfalfa, horsetail, astragalus, gotu kola, feverfew, valerian, hawthorn, rosemary, saw palmetto, ephedra, pau d'arco, ginkgo, garlic, St. John's wort, Agaricus bisporus (common mushroom), Agaricus bisporus brown strain (portabella mushroom), Lentinus edodes (shiitake mushroom), Boletus edulis (porcini mushroom).

The invention discloses a production method for planting agaricus bisporus by using mushroom residues of pleurotus eryngii. The agaricus bisporus consists of the following substances in percentage by weight: 40 to 60 percent of mushroom residues, 20 to 40 percent of corn straws, 10 to 30 percent of cow dung, 1 to 3 percent of urea, 1 to 3 percent of lime and 0.5 to 2.5 percent of calcium superphosphate. In the production method for planting agaricus bisporus by using the mushroom residues of pleurotus eryngii, the routine production process for culturing agaricus bisporus is adopted, wherein the process comprises the following steps of: pre-wetting raw materials, piling the materials, turning over the piles, placing and laying the piled materials on a frame, fermenting the materials, seeding the materials, mycelium culture, earthing, taking out and managing the agaricus bisporus and harvesting fruit bodies. Because the mushroom residues for producing pleurotus eryngii are recycled to serve as the material for planting agaricus bisporus, environmental pollution is avoided and the aim of clean growth and environmental protection is fulfilled.

The invention belongs to the technical field of waste comprehensive utilization of beef cattle farms, and discloses a waste comprehensive utilization process of the large-scale beef cattle farms. The process is characterized by comprising the following steps that (1), cattle feed crop raw materials from farmland are used for preparing organic feed for cattle, and the organic feed is used for cattle farming; (2), cattle dung, urine and flushing water are generated during cattle farming and are collected; 10-90% of cattle dung is used for planting agaricus bisporus, and mushroom dregs generated in the planting process of the agaricus bisporus are used for producing organic fertilizers; the rest of cattle dung, the urine and the flushing water are used for producing biogas and biogas slurry at a biogas station, biogas residues are used for producing organic fertilizers, the biogas slurry is used for farmland irrigation, and the organic fertilizers are used for farmland fertilization. The process not only solves the environmental pollution problem, but also improves the rural energy structure, the mushroom dregs generated in the planting process of the agaricus bisporus and the biogas residues generated by a biogas digester are used for manufacturing the organic fertilizers, the yield of crops is improved obviously, the use number of the fertilizers is reduced, and auxiliary non-point source pollution is relieved.

The invention discloses a flammulina velitupe residue culture medium material and a method for culturing agaricus bisporus. The flammulina velitupe residue culture medium material comprises the following active ingredients in parts by weight: 30-60 parts of flammulina velitupe residues, 35 parts of cow dung, 0.5-1 part of bagasse residues, 0.5-1.5 parts of silkworm faeces, 1 part of compound fertilizer, 1-1.5 parts of lime, 0.5-1 part of gypsum, and 2 parts of calcium superphosphate. The method for culturing agaricus bisporus comprises the steps: piling up, fermenting, sowing, earthing, fruiting and recovering to obtain agaricus bisporus. The fruiting period of the agaricus bisporus is advanced by seven to ten days, the agaricus bisporus recovery rate is prolonged by 10-15 days, the flammulina velitupe residues can be recycled, and the pollution of the flammulina velitupe residue on the environment can be reduced.

The invention discloses a method for extracting agaricus lentinan. The method comprises the following steps: pulping agaricus bisporus; adjusting pH value of the pulp; carrying out composite hydrolysis on the pulp; passivating enzyme; carrying out microwave treatment on the pulp; preserving temperature of the pulp; filtering the pulp; centrifuging the pulp; concentrating the pulp under reduced pressure; removing protein of the pulp by an Sevage method; precipitating alcohol; centrifuging the pulp; filtering the pulp by a membrane; adsorbing the pulp by resin; desorbing the pulp; concentrating the pulp in vacuum; and drying the pulp in vacuum to obtain the agaricus lentinan. The method has the characteristics of high extraction rate and high extracting purity of the agaricus lentinan, and has no emission of pollutant in a technical process, low energy consumption and reasonable design of the process method.

The invention relates to a production method for cultivating agaricus bisporus by using pleurotus eryngii dreg. The production method is characterized in that the cultivation base material comprises the following ingredients in percentage by mass: 50-60% of mushroom dreg, 15-25% of crop waste, 20-30% of livestock excrement, 0.01-0.02% of an auxiliary material calcium superphosphate and 0.05-0.08% of light calcium carbonate. By utilizing the method, not only is the energy waste problem solved, the production cost lowered, the working intensity alleviated, and the fermentation time shortened, but also the nutritive values of the agaricus bisporus are improved, so that the production method can be popularized and used in large scale.

The invention discloses a seasoning powder with natural strong mushroom flavor and a preparation method thereof. The method comprises the steps of using Agaricus bisporus precooked water or extract, condensing, adding a certain amount of monosaccharide, adjusting the pH to be 6-7, and performing the Maillard reaction at 65-75 DEG C for more than 6 hours to obtain a concentrated solution with the natural strong mushroom flavor, then adding maltodextrin, dissolving the maltodextrin completely, performing spray drying to obtain concentrated mushroom powder, and finally, mixing the concentrated mushroom powder, common salt, sodium glutamate, sucrose, flavor-development disodium ribonucleotide, supercritical CO2 extract of spice, calcium phosphate and starch-maltodextrin mixture, adequately agitating until above materials are mixed uniformly, granulating and drying to prepare the seasoning powder with natural strong mushroom flavor.

The invention discloses a straw cultivated Agaricus bisporus formula and a preparation method thereof. The main raw materials consist of straw, dried cow dung, lime, plant ash, calcium superphosphate, gypsum and urea; the conventional cultivated Agaricus bisporus generally uses cotton seed hulls as main cultivation materials, and the purchase of the cotton seed hulls from foreign places has high cost for a plurality of non-cotton-producing areas. The preparation method aims at obtaining the raw materials locally, and the method for the cultivation of the Agaricus bisporus by adding the straw in the cow dung for fermentation can not only save the cost, but also can obtain high yield.

The invention discloses a centralized secondary fermentation method for agaricus bisporus. The culture medium comprises the following raw materials in parts by weight: 45-60 parts of forage, 35-50 parts of chicken manure, 2-8 parts of gypsum and 3-10 parts of oil cakes. The method comprises the following steps of: uniformly spreading forage in a pre-wetting pool for pre-wetting for 2-5 days; adding chicken manure, gypsum and oil cakes and uniformly and adequately mixing; filling the mixed fermentation material in a ventilating floor in a primary fermentation tunnel, and conveying fresh air to the fermentation material through a fan; fermenting for 4-6 days and transferring the fermentation material to another primary fermentation tunnel. The base material of the primary tunnel fermentation is uniformly stacked on the ventilating floor in a secondary fermentation tunnel, and the temperature of the fermentation material is controlled by operation of the fan. Secondary fermentation comprises the following steps: a balancing stage, a heating stage, pasteurization, cultivation at constant temperature and discharge at low temperature. According to the method, local wheat straw hoods, straws, chicken manure and the like are used as main raw materials to form the culture medium. Centralized scaled production cultivation materials are formed by the technology of pre-treatment of the raw materials, primary fermentation and secondary fermentation.

A base material for culturing bisporic mushroom from sewage slag consists of forage, sewage slag, manure powder, ordinary superphosphate, urea, lime powder, gypsum powder and lime carbonate powder. It costs low, has more yield and various material sources, it elongates industrial chain and is beneficial for circulation economics.

The invention relates to an agaricus bisporus casing material and a preparation method thereof. The agaricus bisporus casing material comprises mushroom residue humus, peat or common soil, lime powder and carbendazol. The agaricus bisporus casing material is simple to prepare, low in cost, good in ventilation, water absorption and water retention property and rich in nutrition, the waste of mushroom residue is fully utilized, the usage amount of peat is reduced, and the agaricus bisporus casing material has remarkable economic and ecological benefits.

Belonging to the technical field of edible fungi cultivation, the invention in particular relates to an Agaricus bisporus (Lange) Sing culture medium. The Agaricus bisporus (Lange) Sing culture medium provided in the invention is prepared from the following raw materials by weight: 19-27 parts of chicken manure, 0.30-0.50 part of lime, 28-36 parts of straw, 1.2-2.0 parts of gypsum, 0.15-0.35 part of urea, 0.70-0.90 part of a cake fertilizer, and 0.06-0.14 part of calcium superphosphate. For the medium prepared by the Agaricus bisporus (Lange) Sing fermentation method in the invention, 22kg of Agaricus bisporus (Lange) Sing can be produced by per square meter medium, and is 1-2kg higher than that of other formula medium, wherein the first-class Agaricus bisporus (Lange) Sing accounts for 90%. Single Agaricus bisporus (Lange) Sing has light weight, the Agaricus bisporus (Lange) Sing has good roundness, is easy to transport and store, and can be stored for 4-5d in a cold store at 0DEG C-4DEG C without color change and scales. The price of the Agaricus bisporus (Lange) Sing is higher than those of other fresh mushrooms on the same market by 0.3 yuan per half kilogram, so that the planting benefits are increased.

The invention relates to a method for separating agricus bisporus polysaccharides from agricus bisporus, which comprises the following steps: carrying out vacuum freeze-drying on the agricus bisporus for 24h; extracting crude polysaccharides with hot water: adding distilled water, boiling with big fire, maintaining with slow fire, centrifuging, taking supernatant liquid, precipitating, placing back into a heating device, adding water for continuous heating till boiling, maintaining with the slow fire, re-centrifuging and taking the supernatant liquid; repeating for three times, and using the distilled water for fixing volume of the obtained supernatant liquid for standby; carrying out dual-water phase extraction operation: precisely weighing 2.66g of polyethylene glycol (PEG) 6000 and 4.28g of ammonium sulfate, adding into a 50mL centrifuge tube, adding 20mL of sample diluent, and evenly mixing; simultaneously weighing 2.66g of PEG 6000 and 4.28g of ammonium sulfate, adding into the 50mL centrifuge tube, adding 20mL of distilled water, evenly mixing, and obtaining the agricus bisporus polysaccharides distributed in the PEG phase. The utilization of a dual-water phase system for extracting the agricus bisporus polysaccharides can realize the maximum yield of 80.1%, and the yield can achieve 17.45mg/g.

The invention relates to a method for producing an organic fertilizer by utilizing wastes of materials for planting agaricus bisporus, belongs to the field of agricultural technology and is used for regulating components in the wastes of the materials for planting the agaricus bisporus so as to prepare the organic fertilizer easily absorbed by plants. The organic fertilizer produced by the method provided by the invention comprises the following effective components in parts by weight: the agaricus bisporus wastes, perlite, silica, a water-retaining agent, saw dust and a slow release fertilizer. According to the method, the wastes of the materials for planting the agaricus bisporus are utilized effectively; and the effective components are added. Therefore, nitrogen, phosphorus and potassium and trace elements in the wastes are suitable for plant growth; and the problems of air permeability, water retention and water absorptivity in the wastes are solved. The organic fertilizer is mainly suitable for vegetables, flowers, economic crops, tobaccos and the like.

The invention discloses a method for increasing the yield of agaricus bisporus. The method includes the following steps of raw material preparation, primary fermentation, after fermentation, sowing, earthing management, fruiting management and falling rock discharge, and harvesting. According to the method, an M-shaped bed surface is arranged to increase the batch charging quantity, the heat radiating area and the unit fruiting quantity, explosive fruiting is achieved at the early stage, and finally the purpose of increasing the yield of agaricus bisporus and the purpose of increasing benefits are achieved. By the adoption of the method, resources such as agricultural production waste corncobs and farm animal excrement are fully utilized, environmental pollution is greatly lowered, and the production cost is saved.

The invention belongs to the technical field of edible fungus processing and relates to a preparation method of white mushroom slices. In the method, fresh white mushroom is used as a raw material; and the white mushroom slices are prepared by pretreatment, slicing, soaking, preserving color, killing green in microwaves, cooling, quickly freezing and refrigerating. The white mushroom slice products prepared by the method have natural color, full structure, less moisture loss, less nutrient loss, cooking effect similar to that of fresh white mushroom.