Method for extracting agaricus lentinan

A technology for Agaricus bisporus and polysaccharide, which is applied in the field of microbial chemical macromolecular compound separation, can solve the problems of low efficiency and low purity of Agaricus bisporus polysaccharide, and achieve the effects of high extraction purity, high extraction rate and low energy consumption.

Inactive Publication Date: 2009-12-30
BERLIN
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Problems solved by technology

The efficiency of extracting Agaricus bisporus polysaccharide

Method used

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Embodiment Construction

[0016] Weigh 500 kg of Agaricus bisporus, crush and beat it into a paste, use 10 times pure water to make a concentrated solution, and then adjust the pH value to 5.5-5.6 with citric acid; add 20,000 U of cellulase per gram of Agaricus bisporus , 0.1 million U of hemicellulase, and 0.4 million U of pectinase, stirred for 3 hours at a constant temperature of 45°C for compound enzymolysis, and then instant inactivated enzyme treatment at 100°C; the concentrated solution after compound enzymolysis was placed into the microwave instrument, and carry out 3 intermittent microwave treatments, each microwave treatment time is 2 seconds, the intermittent time is 35 seconds, and the power of the microwave instrument is 500W; the liquid after the microwave treatment is placed in a 55°C water bath for 30 minutes, Squeeze and filter with a filter cloth, wash the filter residue with hot distilled water, combine the filtrate, and centrifuge the filtrate for 10 minutes, the speed of the centri...

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Abstract

The invention discloses a method for extracting agaricus lentinan. The method comprises the following steps: pulping agaricus bisporus; adjusting pH value of the pulp; carrying out composite hydrolysis on the pulp; passivating enzyme; carrying out microwave treatment on the pulp; preserving temperature of the pulp; filtering the pulp; centrifuging the pulp; concentrating the pulp under reduced pressure; removing protein of the pulp by an Sevage method; precipitating alcohol; centrifuging the pulp; filtering the pulp by a membrane; adsorbing the pulp by resin; desorbing the pulp; concentrating the pulp in vacuum; and drying the pulp in vacuum to obtain the agaricus lentinan. The method has the characteristics of high extraction rate and high extracting purity of the agaricus lentinan, and has no emission of pollutant in a technical process, low energy consumption and reasonable design of the process method.

Description

technical field [0001] The invention relates to a method for extracting Agaricus bisporus polysaccharide, which belongs to the separation technology of microbial chemical macromolecular compounds. Background technique [0002] Agaricus burnnescens peck, scientific name mushroom, white mushroom or mushroom, belongs to Fungiphylum, Basidiomycotina, Laminomycetes, Agaricaceae, and Mushroomaceae; it is a wild edible fungus with both food and medicine. It has the characteristics of high protein, low fat, and a complete range of amino acids. It also has the effects of benefiting the spleen, clearing the heart and calming the nerves, helping food, and curing hemorrhoids. It also has the effect of regulating human metabolism and enhancing human physique; It is an important component and energy substance, and also has various physiological functions such as immunity and anti-cancer. [0003] Chinese Patent Application No. 200810222270.1 discloses "a Agaricus bisporus extract and its...

Claims

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Application Information

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IPC IPC(8): C08B37/00C12S3/02
Inventor 柏林
Owner BERLIN
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