The invention discloses an
exosome extraction method based on
plant juice characteristics. The method comprises the following steps: firstly, according to the characteristics of
plant juice, dividing samples into four types, namely a clear low-
viscosity type sample, a medium-thick type sample, a high-
viscosity type sample and a high-
fiber type sample, and adopting different pretreatment
modes: washing the clear type sample,
cutting into blocks, and directly juicing; performing enzymolysis after medium-concentration washing and juicing; performing enzymolysis after high-
viscosity homogenate juicing; and the high-
fiber type is firstly subjected to enzymolysis and
softening, and then homogenate and squeeze juice. Removing impurities through two-step
differential centrifugation, and finally obtaining the high-purity
exosome through
ultrafiltration concentration, PEG
precipitation, PBS resuspension and
filtration. According to the method disclosed by the invention, various means such as physical crushing, enzymolysis,
differential centrifugation,
ultrafiltration and
precipitation are comprehensively utilized, so that the yield and purity of the
exosome are greatly improved, the varieties of plants for preparing the exosome are widened, and a
technical support is provided for application research of the
plant exosome.