49 results about "Eggshell membrane" patented technology

The invention discloses an eggshell membrane peptide for enhancing bone mineral density as well as a preparation method and application thereof, belongs to the technical field of egg by-product processing, and particularly relates to a preparation method of the eggshell membrane peptide for enhancing bone mineral density, which comprises the following steps: carrying out vacuum freeze drying and crushing on an eggshell membrane to obtain eggshell membrane powder; mixing the eggshell membrane powder with deionized water to obtain an eggshell membrane solution; adding keratinase, performing enzymolysis, and taking supernate; adding collagenase, carrying out enzymolysis, and centrifuging to take supernate, so as to obtain primary enzymatic hydrolysate; adding alkaline phosphatase, performing enzymolysis and centrifugation, and taking supernate to obtain enzymatic hydrolysate; and performing ultrafiltration and drying to obtain the eggshell membrane peptide. According to the eggshell membrane peptide for enhancing the bone mineral density, keratin cross-linking is destroyed through enzymolysis of keratinase, active peptide capable of stimulating osteoblast proliferation is released through enzymolysis of collagenase, phosphopeptide calcium binding sites are further activated through enzymolysis of alkaline phosphatase, calcium salt deposition is promoted, and the eggshell membrane peptide for enhancing the bone mineral density is provided.

The application belongs to the field of biomedical materials and biomedical engineering technology, and discloses a pH-responsive curcumin sustained-release microsphere hydrogel, a preparation method and application thereof in spinal cord injury repair materials. The preparation method is as follows: short fibers are prepared by pretreating and grinding discarded eggshell membranes; after sodium alginate is dissolved, the sodium alginate is blended with the eggshell membrane short fibers, curcumin is added, and stirring is uniformly performed to form a mixed solution; the mixed solution is injected into a calcium chloride solution by using a drip crosslinking method to form, and an eggshell membrane and sodium alginate curcumin microsphere hydrogel is obtained. The hydrogel has excellent water content, swelling performance, biocompatibility, blood compatibility and drug sustained-release function, and can be applied to spinal cord injury repair.

The invention belongs to the technical field of biological medicines, and particularly discloses an anti-fatigue and joint protection composition for sports people, which comprises the following components in percentage by mass: 10-25% of citrus bioflavonoid, 5-15% of taurine, 15-30% of eggshell membrane powder, 2-8% of curcumin compound, 1-5% of citric acid and the balance of maltooligosaccharide. Under the synergistic interaction of the citrus bioflavonoid, the taurine, the eggshell membrane powder and the curcumin compound, the composition with excellent anti-fatigue and joint protection effects is obtained.

The present application discloses a kind of eggshell membrane water solution device and the production method using it, the device includes multiple containers, a liquid supply unit, a heating unit, a condensation recovery unit, a hanging unit, a microwave generating unit and a control unit, multiple the container is used to install different units respectively and is combined in stack mode, to make an eggshell raw material, a clean water and an enzyme in the container inside, heated to 50~70 DEG C for 6~12 hours, water vapor in the process is collected to become recovery water, the recovery water is used to flush the eggshell raw material to wash the water-soluble eggshell membrane remaining on the surface, air extraction and heating to 60~80 DEG C are carried out to reduce pressure concentration, finally using microwave destroys the protein bond of the egg membrane water solution and makes the enzyme inactivation;The foregoing action flow is automated to improve product quality and efficiency, and stack design can also improve the flexibility of configuration.

To provide frozen noodles causing little evaporation of water from the noodles after boiled while improving the production efficiency of the frozen noodles, having firm texture to such an extent that the core does not remain when eaten, and having smooth texture on the surface.SOLUTION: It has been found that when a lysyl oxidase derived from an eggshell membrane is used, noodles having no remaining core and having firm texture are obtained even after a short boiling time, the noodles hardly spread even after a prolonged boiling time, and the surface of the noodles after washing with water is smooth without unevenness and excellent in slipperiness.SELECTED DRAWING: None

The present application relates to the field of eggshell membrane peptide, in order to solve the problem that the proportion of polypeptide with main function of anti-inflammatory effect is low in the eggshell membrane peptide product obtained by hydrolysis of existing eggshell membrane, provide an eggshell membrane peptide and a preparation method thereof, and an application of the eggshell membrane peptide in preparation of anti-inflammatory drugs, comprising: S100, after adding eggshell membrane powder into pure water, adjusting pH, then adding pepsin, enzymolysis for 2-5h, and the enzymolysis temperature is 35-38 DEG C, to obtain a first mixture; S200, the first mixture is subjected to enzyme inactivation process and ultrafiltration process to obtain a first hydrolysate and a first ultrafiltrate; S300, after adjusting the pH value of the first ultrafiltrate to 8-9, adding alkaline protease, enzymolysis for 6-10h, and the enzymolysis temperature is 36-38 DEG C, to obtain a second mixture; S400, the second mixture is subjected to ultrafiltration process to obtain a second ultrafiltrate and a second hydrolysate; S500, after drying the second hydrolysate, a first polypeptide composition is obtained. The present application explores the effect of different hydrolysate components on chondrocyte anti-inflammatory, and screens out eggshell membrane peptides with better effect.

The application discloses a preparation method of small molecule peptides based on synergistic degradation of chicken eggshell membranes by molds and the small molecule peptides, and relates to the field of small molecule peptide preparation. The method comprises the following steps: mixing pretreated chicken eggshell membranes with mold fermentation liquor, and performing synergistic degradation; inactivating and centrifuging the degraded mixture, and collecting supernatant; purifying small molecule peptides in the supernatant through membrane separation technology; drying the purified small molecule peptide solution through a low-temperature spray drying method to obtain small molecule peptide powder; and the molds include Trichoderma reesei and Aspergillus niger. Through synergistic degradation of the two molds, the conversion of the chicken eggshell membranes is more thorough, the yield of the small molecule peptides is significantly improved, the total nitrogen recovery rate can reach 85.2%, and the product molecular weight distribution is concentrated.

The present invention provides a guided bone regeneration (GBR) material with antibacterial properties. The GBR material is a multi-component material, including an eggshell membrane (ESM) which is functionalized by mineralization in the presence of low-molecular-weight polyacrylic acid (LPAA). Thus, the ESM therefore includes at least one mineral deposited therein, preferably, intrafibrillar deposition, for example apatite and effective amounts of LPAA. The mineralized GBR material is preferably porous, and has good mechanical properties. A method of making mineralized GBR material which preferably contains intrafibrillar mineralization is also provided.

The application discloses an eggshell membrane peptide with antioxidant and improving exercise capacity of aging organism and application thereof, and belongs to the technical field of biology. The amino acid sequence of the eggshell membrane peptide is shown as SEQ ID NO. 1, and is specifically EQPWFPAG. A novel antioxidant peptide EQPWFPAG is identified from chicken eggshell membrane hydrolysate, and the eggshell membrane peptide can improve the exercise capacity and antioxidant stress capacity of an aging organism through verification. The eggshell membrane peptide provided by the application can be used for preparing an antioxidant, and can also be used for preparing functional food, health products and pharmaceutical compositions for improving the exercise function of an aging organism, and has great application value.

The invention relates to the technical field of biological engineering, and discloses eggshell membrane powder and a preparation method thereof, and the preparation method comprises the following steps: (1) adopting a fermentation broth of Bacillus licheniformis CICC No: 10092 as an enzyme preparation, and carrying out an enzymatic hydrolysis reaction on eggshells with membranes; (2) after the enzymolysis is finished, performing enzyme deactivation, and filtering enzymatic hydrolysate to obtain supernate; and (3) carrying out spray drying on the supernate, and collecting dried powder, namely the eggshell membrane powder. The preparation method solves the problems of complicated operation, chemical reagent residues, high difficulty in multi-enzyme synergistic regulation and the like in the existing preparation technology of the eggshell membrane powder, and the content of active ingredients such as collagen, hyaluronic acid and chondroitin sulfate in the prepared eggshell membrane powder is equivalent to that in the prior art; and the method has the outstanding advantages of simplified process, greenness, safety and controllable cost.

The present invention relates to a composition comprising a peptide fraction, wherein: the peptide fraction comprises cysteine and methionine; the mass content of methionine of the peptide fraction is greater than 1% relative to the total amino acids of the peptide fraction; the cysteine of the peptide fraction is at least partially sulfonated, the degree of sulfonation of the cysteine being greater than 50%, preferably greater than 70%, and even more preferably greater than 80%.

The present invention relates to a composition and method for solubilizing eggshell membrane at convenient rates. The present invention also relates to organoleptically acceptable products obtained therein, and to their oral or topical application to improve a disease or condition in a subject.

The application belongs to the technical field of feed additives, and provides a mixed feed additive for improving immunity of an animal body and a preparation method thereof.The mixed feed additive for improving immunity of an animal body comprises the following components in parts by weight: 20-25 parts of enzymolysis eggshell membrane powder, 38-42 parts of enzymolysis bamboo fiber, 0.5-0.8 parts of probiotics, 10-15 parts of beet powder, 8-12 parts of psyllium powder and 4-6 parts of dandelion powder.The application can effectively enhance the immunity of the animal body.

The present application relates to the technical field of all-solid-state lithium metal battery, and particularly relates to a solid-state polymer electrolyte and a preparation method and application thereof. In the present application, trifluoroethanol is used to fluorinate eggshell membrane powder in natural eggshell, which is used as a polyethylene oxide-based solid-state polymer electrolyte additive. This can adjust the network structure of the polymer, enhance the electrochemical and mechanical performance of the solid-state electrolyte, inhibit lithium dendrite growth, and enhance the cycle performance of the lithium metal battery. The lithium ion transfer number of the improved solid-state electrolyte battery is determined by chronamperometry, and the lithium ion transfer number is higher, and it is easier to generate fluorinated lithium and lithium oxide, and better inhibit the growth of lithium dendrites. The addition of trifluoroethanol to fluorinate the eggshell membrane solid-state electrolyte in the natural eggshell makes the lithium symmetrical battery and the lithium iron phosphate full battery exhibit excellent performance in various cases.

The application discloses an eggshell membrane steam explosion pretreatment device and method and the technical field of steam explosion, and comprises a steam explosion tank, a high-pressure steam supplier, a rapid pressure relief device, a mechanical crushing device, a pre-crushing module and a separation screening module. The application loosens the material in advance through the double-toothed roll crusher of the pre-crushing module, uses high-pressure steam explosion to combine the rapid pressure relief device with extremely fast response to generate a high-speed airflow to tear the eggshell membrane, and uses the pressure relief airflow to drive the built-in radial flow impeller to rotate at high speed, the transmission shaft drives the multi-layer staggered cutter head with a high-hardness cubic boron nitride coating, and cooperates with the fixed stepped tooth plate to realize efficient recycling of the pressure relief energy under the stability of the speed-regulating flywheel, significantly reduces external energy consumption, and integrates the pre-crushing, steam explosion, energy recovery crushing and subsequent separation screening modules into one, greatly improves the efficiency, automation degree and final product yield and quality of the eggshell membrane separation treatment.

The invention discloses an eggshell and shell membrane efficient smashing and separating device and method, and relates to the technical field of eggshell treatment.The eggshell and shell membrane efficient smashing and separating device comprises a base, a separating mechanism and a discharging mechanism, a separating tank is arranged on the base, an eggshell membrane collecting tank is arranged on the upper side of the separating tank, and the separating mechanism is arranged in the separating tank on the base; the separation mechanism comprises bearing seats and a separation barrel, first rotating rods are fixedly connected to the two sides of the separation barrel, the bearing seats are symmetrically arranged in the separation tank, the first rotating rods are located on the bearing seats, and a discharging mechanism is arranged on one side of the separation tank. Meanwhile, a second rotating rod drives a turbulent flow piece on a rotating wheel to generate turbulent flow on the eggshells, auger blades whip the eggshells to achieve preliminary separation of shell membranes, coarsely broken eggshells fall into the lower layer through a filter screen to be collected, positive pressure air enters through a fan and is blown out upwards through the filter screen, and the eggshell membranes are prevented from being mixed into the eggshells.

The application belongs to the field of biomedical materials and biomedical engineering technology, and discloses a photothermal responsive curcumin sustained-release microgel as well as a preparation method and application thereof. The preparation method is as follows: eggshell membrane short fibers are soaked in a dopamine aqueous solution, centrifuged and washed after dark storage, and dopamine-eggshell membrane short fibers are obtained; the dopamine-eggshell membrane short fibers, curcumin and sodium alginate are mixed, dropped into a calcium chloride solution for shaping, and a photothermal responsive curcumin sustained-release microgel is obtained. The microgel has excellent water content, compact microstructure, biocompatibility, blood compatibility and photothermal response function, and can be applied to precise and controllable drug loading.

The invention provides a membrane phospholipid composition specific liposome for promoting eggshell mineralization and application thereof. The liposome membrane is prepared from the following components as raw materials: (a) core membrane phospholipid; (b) an acidic phospholipid and (c) a membrane stabilizing component; wherein the core membrane phospholipid comprises PC (Polycarbonate) and PE (Polyethylene); the acidic phospholipid is PS and / or PA; the membrane stabilizing component comprises at least one of methoxy polyethylene glycol modified phosphatidyl ethanolamine (DSPE-PEG), 1, 2-dimyristoyl-rac-glycerol-3-methoxy polyethylene glycol (DMG-PEG), 1, 2-dioleoyl-sn-glycerol-3-phosphoethanolamine-N-[methoxy (polyethylene glycol)] (DOPE-PEG), and cholesterol (Cholesterol). Through specific combination of core membrane phospholipids (PC and PE) and acidic phospholipids (PS and / or PA), an initial mineralization microenvironment in a living body is successfully simulated. In-vitro mineralization test results show that the liposome can effectively promote formation and growth of calcium carbonate crystal nucleuses, has excellent performance on interfaces of eggshell membranes, silicon wafers and the like, and proves universality and effectiveness.

A composition which is a hydrogel including, in an aqueous medium: at least one hydrophilic thickening polymer, and particles of eggshell membrane, in an amount of less than 5% by weight of the total weight of the composition, wherein the particles are rod-shaped, needle-shaped or fibrous, having a specific particle size. Also described is a bioink including the composition, a method for manufacturing a 2D or 3D product using the hydrogel, a kit including the hydrogel or bioink, and to uses thereof.

The invention discloses a poultry eggshell membrane steam explosion pretreatment device and method, and relates to the technical field of steam explosion. The poultry eggshell membrane steam explosion pretreatment device comprises a steam explosion tank, a high-pressure steam supplier, a rapid pressure relief device, a mechanical crushing device, a pre-crushing module and a separating and screening module. Materials are loosened in advance through a double-geared-roller crusher of the pre-crushing module, high-pressure steam explosion is combined with a quick pressure relief device which is extremely quick in response to generate instantaneous high-speed airflow to tear eggshell membranes, and pressure relief airflow is used for driving a built-in radial flow type impeller to rotate at a high speed; the transmission shaft drives the multiple layers of staggered cutterheads with high-hardness cubic boron nitride coatings and cooperates with the fixed stepped toothed plate, under the condition that the speed regulation flywheel is stable, efficient recycling of pressure relief energy is achieved, external energy consumption is remarkably reduced, meanwhile, a pre-crushing module, a steam explosion module, an energy recycling and crushing module and a subsequent separation and screening module are integrated, and the production efficiency is improved. The efficiency and the automation degree of eggshell membrane separation treatment as well as the yield and the quality of final products are greatly improved.

The invention relates to the technical field of pet food processing, and particularly provides a protein-lipid compound oil gel coating composition which comprises the following components in parts by weight: 60-70 parts of egg yolk oil; 10 to 20 parts of eggshell membrane peptide; 5-10 parts of medium-chain fatty acid; 0.1 to 0.5 part of vitamin E; 10 to 15 parts of ethyl cellulose; and 2-5 parts of nano silicon dioxide. Egg oil is used as a substrate, an oil gel three-dimensional network is constructed, eggshell membrane protein is integrated into lipid, and precise function delivery of joint and fur health is achieved. The solid coating is stable in structure and high in oxidation resistance, can be firmly attached to the surface of the pet food, effectively prevents grease oxidation and leakage, and remarkably prolongs the shelf life and improves the additional value of the product.

The invention discloses a soil conditioner for improving a soil structure, and belongs to the technical field of soil conditioning. The soil conditioner comprises the following raw materials in parts by weight: 60-70 parts of a fermentation base material, 6-10 parts of fulvic acid, 1-5 parts of polyacrylamide, 10-15 parts of a nano humic acid capsule, 8-12 parts of konjac-PAM gel and 1-3 parts of bentonite, the nano humic acid capsule is of a core-shell structure formed by wrapping fulvic acid and nano zinc oxide with an eggshell membrane, and the nano humic acid capsule is of a hollow structure. The konjac-PAM gel is obtained by carrying out graft copolymerization on konjac glucomannan and N-isopropylacrylamide. The soil conditioner provided by the invention can improve the soil structure, significantly improve the soil fertility and the crop growth efficiency, reduce the poison of heavy metals to crops, maintain the root health of the crops, significantly improve the air permeability, and relieve waterlogging and drought.

The invention relates to the technical field of soft boiled egg processing, and discloses a self-adaptive soft boiled egg stripping method and system and a storage medium, and the method comprises the following steps: obtaining a near infrared spectrum signal and an ultrasonic echo signal of an eggshell membrane through a sensing module; calculating a chemical component evaluation value reflecting the chemical component change of the film, and calculating a physical structure evaluation value reflecting the physical structure change of the film; calculating a comprehensive demolding state evaluation value S; based on the comprehensive evaluation value S, the pH value, the temperature and the enzyme concentration of the treating fluid are subjected to coupling adjustment. The dynamic change of the eggshell membrane is comprehensively captured in real time, the membrane removing process can be more accurately controlled through coupling adjustment, membrane residues or egg body damage is avoided, the one-time membrane removing success rate and the consistency of the product quality are remarkably improved, and the problems that the membrane removing efficiency is low and the product quality is unstable due to the lack of dynamic adjustment capacity are solved.

The invention discloses an extraction and activity protection method for functional components in an eggshell membrane. The method comprises the steps of raw material pretreatment and cryogenic superfine grinding, ultrasonic-assisted swelling, compound enzyme step-by-step targeted extraction, separation and purification, multi-source activity protection and the like. The method has the remarkable effects that the method is realized by an integrated technology of copious cooling superfine grinding, ultrasonic assistance, compound enzyme step-by-step targeted extraction and multi-element activity protection, the extraction efficiency of functional components is extremely high, the product activity is excellent, the molecular weight distribution of the extracted collagen is concentrated, and the collagen can be effectively extracted by virtue of a multi-source activity protection measure. The industrial problem that active ingredients are prone to inactivation is systematically solved, the product can be applied to different application scenes such as health care products, cosmetics and biological medicine, and high-value utilization of agricultural waste is achieved.

The application discloses a gel microsphere and a preparation method and application thereof, and is characterized in that natural high-molecular chitosan is used as a matrix, eggshell membranes are added to control the pore structure of the trapping gel material, the high-molecular chitosan and the eggshell membranes used in the application are both natural biomass, have a high potential response to CO2, are renewable and degradable, are rich in resources, are low in cost, and are non-toxic, harmless and non-polluting.

The invention belongs to the technical field of skin dressings, and particularly relates to an eggshell membrane oxidation regenerated cellulose nanofiber membrane and a preparation method and application thereof. The preparation method comprises the following steps: preparing a substrate film layer from a substrate spinning solution by adopting a microfluidic gas jet spinning method; preparing the composite spinning solution on the surface of the substrate fiber membrane by adopting a microfluidic gas jet spinning method to obtain a medicine-containing membrane layer; the eggshell membrane oxidation regenerated cellulose nanofiber membrane is composed of the base membrane layer and the medicine-containing membrane layer. According to the eggshell membrane oxidized regenerated cellulose nanofiber membrane obtained by the preparation method provided by the invention, the dissolution rate and bioavailability of a medicine are remarkably improved, and the preparation method has excellent process reproducibility, so that the stability of product quality and the consistency between batches are ensured; and a reliable technical support is provided for industrial production of the eggshell membrane oxidation regenerated cellulose nanofiber membrane as a skin auxiliary material.

The invention relates to the technical field of health-care food, and discloses a male reproductive health complex formula health-care product containing egg membrane peptide. Comprising the following raw materials in parts by weight: 10-50 parts of egg membrane peptide, 5-20 parts of a zinc compound, 5-15 parts of vitamin E, 1-10 parts of lycopene, 5-25 parts of pachymaran, 3-12 parts of coenzyme Q10, 5-20 parts of a saw palmetto extract, 5-15 parts of L-carnitine, 10-30 parts of vitamin C, 3-10 parts of a ginkgo biloba extract, 5-20 parts of a maca extract, 5-25 parts of lycium barbarum polysaccharide and 1-8 parts of astaxanthin. The egg membrane peptide is a polypeptide composition prepared from an egg shell membrane through an enzymolysis or fermentation method. The zinc compound is one or more of zinc citrate, zinc glycinate and zinc gluconate. The complex formula has a synergistic effect from multiple dimensions of oxidation resistance, inflammation resistance, energy supply and endocrine regulation, and the concentration, activity, normal morphological rate and DNA integrity of sperms are improved.

This invention belongs to the technical field of bone and joint health products, and provides a composition for improving osteoarthritis, its preparation method, application, and product. The composition comprises, by weight, 1-300 parts mangosteen fruit powder, 1-150 parts eggshell membrane, and 1-250 parts turmeric. The composition of this invention is simple in composition, and through the compounding of mangosteen fruit powder, eggshell membrane, and turmeric in a specific ratio, it shows significant effects in repairing osteoarthritis.

The present application relates to a kind of imitation eggshell membrane structure composite microporous membrane surface decoration's microporous foaming injection molding product manufacturing method and product.Prepare microporous membrane as outer layer material, melt-blown nonwoven fabric as inner layer material;Inner and outer layer are stacked, and imitation eggshell membrane structure composite microporous membrane is prepared using heat treatment and hot pressing molding;Composite microporous membrane is embedded in mold and implements microporous foaming injection molding of composite microporous membrane surface decoration, and product is prepared.In molding, by the selective permeability of outer layer microporous membrane and the penetration of foaming melt and inner layer nonwoven fabric reticular fiber, membrane-base interface gas escape channel is established, gas trapped in membrane-base interface is effectively discharged, and close interface interlocking structure is formed, solve the common internal surface decoration microporous foaming molding plastic piece membrane-base interface produces bulge, separation defect and low bonding strength problem.By the present application, lightweight plastic product with high apparent quality and high bonding strength is prepared at low cost and high efficiency, and has wide application prospect.

The invention provides a biological membrane base material, a preparation method and application, and relates to the technical field of biological materials. The biological membrane base material is prepared by mixing and cross-linking carrageenan, red algae glue, eggshell membrane powder and glycerol and carrying out post-treatment on the carrageenan, the red algae glue, the eggshell membrane powder and the glycerol. The tear resistance is greater than 40N / mm < 2 >, and the requirements of medical dressing are met; the transdermal absorption rate of the active ingredients is increased to 20% or above; the raw materials are all natural components, are non-irritant and can be completely degraded in the natural environment or in the body, and the pollution problem of a traditional plastic film is solved.