70 results about "Eggshell membrane" patented technology

The invention discloses a method for promoting dissolution of eggshell membrane protein and preparing chelated calcium through high-pressure crushing. Comprising the following steps: separating and drying eggshells and eggshell membranes of egg shells, and respectively carrying out superfine grinding to obtain eggshell powder and eggshell membrane powder; adding water into the eggshell membrane powder, carrying out high-pressure homogenization, and centrifuging to obtain an eggshell membrane solution; adding eggshell powder into the eggshell membrane solution, adjusting the pH value to 1-3, and adding pepsase for reaction; after the reaction, adjusting the pH value of the eggshell membrane solution to 6.5-7.5, and adding trypsin for reaction; and after the reaction, inactivating the enzyme, centrifuging to take a supernatant, adding absolute ethyl alcohol into the supernatant for alcohol precipitation, and centrifuging to obtain a precipitate, namely the chelated calcium. The technical problem that the eggshell membrane protein is difficult to dissolve out is solved, and a new way is provided for industrial extraction of the eggshell membrane soluble protein.

The invention discloses an eggshell membrane peptide for enhancing bone mineral density as well as a preparation method and application thereof, belongs to the technical field of egg by-product processing, and particularly relates to a preparation method of the eggshell membrane peptide for enhancing bone mineral density, which comprises the following steps: carrying out vacuum freeze drying and crushing on an eggshell membrane to obtain eggshell membrane powder; mixing the eggshell membrane powder with deionized water to obtain an eggshell membrane solution; adding keratinase, performing enzymolysis, and taking supernate; adding collagenase, carrying out enzymolysis, and centrifuging to take supernate, so as to obtain primary enzymatic hydrolysate; adding alkaline phosphatase, performing enzymolysis and centrifugation, and taking supernate to obtain enzymatic hydrolysate; and performing ultrafiltration and drying to obtain the eggshell membrane peptide. According to the eggshell membrane peptide for enhancing the bone mineral density, keratin cross-linking is destroyed through enzymolysis of keratinase, active peptide capable of stimulating osteoblast proliferation is released through enzymolysis of collagenase, phosphopeptide calcium binding sites are further activated through enzymolysis of alkaline phosphatase, calcium salt deposition is promoted, and the eggshell membrane peptide for enhancing the bone mineral density is provided.

The invention discloses an antioxidant and firming polypeptide composition and a preparation method thereof, and the antioxidant and firming polypeptide composition comprises the following components in percentage by weight: 50-75% of water, 5-10% of butanediol, 0.1-3% of panthenol and 0.5-1% of p-hydroxyacetophenone. 0.2 to 8% of hydroxyethyl piperazine ethanesulfonic acid; the composition is prepared from the following components in percentage by weight: 20-30% of an eggshell membrane extract, 0.01-2% of nonapeptide-1, 0.01-5% of acetyl hexapeptide-8 and 0.5-1% of 1, 2-hexanediol; the preparation method comprises the following steps: putting water, butanediol, panthenol and p-hydroxyacetophenone into a container, and stirring at a constant temperature to obtain a first mixed solution; adding hydroxyethyl piperazine ethanesulfonic acid into the first mixed solution, and uniformly stirring to obtain a second mixed solution; adding an eggshell membrane extract, nonapeptide-1, acetyl hexapeptide-8 and 1, 2-hexanediol into the second mixed solution, and uniformly stirring to obtain the antioxidant and firming polypeptide composition; the antioxidant and firming polypeptide composition disclosed by the invention has the functions of resisting oxidation, firming and brightening the skin; the method for preparing the antioxidant and firming polypeptide composition is simple and convenient for large-scale production.

The application belongs to the field of biomedical materials and biomedical engineering technology, and discloses a pH-responsive curcumin sustained-release microsphere hydrogel, a preparation method and application thereof in spinal cord injury repair materials. The preparation method is as follows: short fibers are prepared by pretreating and grinding discarded eggshell membranes; after sodium alginate is dissolved, the sodium alginate is blended with the eggshell membrane short fibers, curcumin is added, and stirring is uniformly performed to form a mixed solution; the mixed solution is injected into a calcium chloride solution by using a drip crosslinking method to form, and an eggshell membrane and sodium alginate curcumin microsphere hydrogel is obtained. The hydrogel has excellent water content, swelling performance, biocompatibility, blood compatibility and drug sustained-release function, and can be applied to spinal cord injury repair.

The invention belongs to the technical field of biological medicines, and particularly discloses an anti-fatigue and joint protection composition for sports people, which comprises the following components in percentage by mass: 10-25% of citrus bioflavonoid, 5-15% of taurine, 15-30% of eggshell membrane powder, 2-8% of curcumin compound, 1-5% of citric acid and the balance of maltooligosaccharide. Under the synergistic interaction of the citrus bioflavonoid, the taurine, the eggshell membrane powder and the curcumin compound, the composition with excellent anti-fatigue and joint protection effects is obtained.

The present application discloses a kind of eggshell membrane water solution device and the production method using it, the device includes multiple containers, a liquid supply unit, a heating unit, a condensation recovery unit, a hanging unit, a microwave generating unit and a control unit, multiple the container is used to install different units respectively and is combined in stack mode, to make an eggshell raw material, a clean water and an enzyme in the container inside, heated to 50~70 DEG C for 6~12 hours, water vapor in the process is collected to become recovery water, the recovery water is used to flush the eggshell raw material to wash the water-soluble eggshell membrane remaining on the surface, air extraction and heating to 60~80 DEG C are carried out to reduce pressure concentration, finally using microwave destroys the protein bond of the egg membrane water solution and makes the enzyme inactivation;The foregoing action flow is automated to improve product quality and efficiency, and stack design can also improve the flexibility of configuration.

To provide frozen noodles causing little evaporation of water from the noodles after boiled while improving the production efficiency of the frozen noodles, having firm texture to such an extent that the core does not remain when eaten, and having smooth texture on the surface.SOLUTION: It has been found that when a lysyl oxidase derived from an eggshell membrane is used, noodles having no remaining core and having firm texture are obtained even after a short boiling time, the noodles hardly spread even after a prolonged boiling time, and the surface of the noodles after washing with water is smooth without unevenness and excellent in slipperiness.SELECTED DRAWING: None

The present application relates to the field of eggshell membrane peptide, in order to solve the problem that the proportion of polypeptide with main function of anti-inflammatory effect is low in the eggshell membrane peptide product obtained by hydrolysis of existing eggshell membrane, provide an eggshell membrane peptide and a preparation method thereof, and an application of the eggshell membrane peptide in preparation of anti-inflammatory drugs, comprising: S100, after adding eggshell membrane powder into pure water, adjusting pH, then adding pepsin, enzymolysis for 2-5h, and the enzymolysis temperature is 35-38 DEG C, to obtain a first mixture; S200, the first mixture is subjected to enzyme inactivation process and ultrafiltration process to obtain a first hydrolysate and a first ultrafiltrate; S300, after adjusting the pH value of the first ultrafiltrate to 8-9, adding alkaline protease, enzymolysis for 6-10h, and the enzymolysis temperature is 36-38 DEG C, to obtain a second mixture; S400, the second mixture is subjected to ultrafiltration process to obtain a second ultrafiltrate and a second hydrolysate; S500, after drying the second hydrolysate, a first polypeptide composition is obtained. The present application explores the effect of different hydrolysate components on chondrocyte anti-inflammatory, and screens out eggshell membrane peptides with better effect.

The application discloses a preparation method of small molecule peptides based on synergistic degradation of chicken eggshell membranes by molds and the small molecule peptides, and relates to the field of small molecule peptide preparation. The method comprises the following steps: mixing pretreated chicken eggshell membranes with mold fermentation liquor, and performing synergistic degradation; inactivating and centrifuging the degraded mixture, and collecting supernatant; purifying small molecule peptides in the supernatant through membrane separation technology; drying the purified small molecule peptide solution through a low-temperature spray drying method to obtain small molecule peptide powder; and the molds include Trichoderma reesei and Aspergillus niger. Through synergistic degradation of the two molds, the conversion of the chicken eggshell membranes is more thorough, the yield of the small molecule peptides is significantly improved, the total nitrogen recovery rate can reach 85.2%, and the product molecular weight distribution is concentrated.

The invention belongs to the technical field of functional foods and health-care foods, and particularly relates to a composition with an effect of preventing and / or improving non-alcoholic fatty liver and a preparation method of the composition. The composition comprises an eggshell membrane extract, lemon concentrated powder, ganoderma sinensis powder and beet root powder. The composition prepared by the invention can be used for effectively relieving liver injury, liver lipid metabolism disorder and liver oxidative stress by reducing the liver index and weight change rate of a mouse, reducing the contents of AST and ALT in serum of the mouse and the contents of TC, TG and MDA in the liver and improving the content of SOD in the liver, so that the effect of improving the non-alcoholic fatty liver is achieved.

The present invention provides a guided bone regeneration (GBR) material with antibacterial properties. The GBR material is a multi-component material, including an eggshell membrane (ESM) which is functionalized by mineralization in the presence of low-molecular-weight polyacrylic acid (LPAA). Thus, the ESM therefore includes at least one mineral deposited therein, preferably, intrafibrillar deposition, for example apatite and effective amounts of LPAA. The mineralized GBR material is preferably porous, and has good mechanical properties. A method of making mineralized GBR material which preferably contains intrafibrillar mineralization is also provided.

The application discloses an eggshell membrane peptide with antioxidant and improving exercise capacity of aging organism and application thereof, and belongs to the technical field of biology. The amino acid sequence of the eggshell membrane peptide is shown as SEQ ID NO. 1, and is specifically EQPWFPAG. A novel antioxidant peptide EQPWFPAG is identified from chicken eggshell membrane hydrolysate, and the eggshell membrane peptide can improve the exercise capacity and antioxidant stress capacity of an aging organism through verification. The eggshell membrane peptide provided by the application can be used for preparing an antioxidant, and can also be used for preparing functional food, health products and pharmaceutical compositions for improving the exercise function of an aging organism, and has great application value.

The invention belongs to the technical field of nutritional health-care food and biological medicine, and particularly relates to a composite peptide composition with bone and cartilage growth promoting ability and a preparation method, application and a product of the composite peptide composition. The composite peptide composition provided by the invention consists of marine fishbone collagen peptide, chondropeptide and eggshell membrane peptide. The composite peptide composition and the composite peptide preparation are good in safety, and the survival rate of chondrocytes and osteoblasts treated by hydrogen peroxide can be remarkably increased. Gene expressions of ACAN, COL1 alpha1, COL2 alpha1, CHST3, HAS3 and OCN in chondrocytes and osteoblasts are up-regulated, and it is prompted that the compound has protection and promotion effects on bone-related cell functions. The composite peptide composition and the composite peptide preparation synchronously act on bone longitudinal growth and cartilage health through multi-target design, provide comprehensive nutrition support for bone and cartilage synergistic development, and have the characteristics of high safety, comprehensive functions and good palatability.

The invention relates to the technical field of biological engineering, and discloses eggshell membrane powder and a preparation method thereof, and the preparation method comprises the following steps: (1) adopting a fermentation broth of Bacillus licheniformis CICC No: 10092 as an enzyme preparation, and carrying out an enzymatic hydrolysis reaction on eggshells with membranes; (2) after the enzymolysis is finished, performing enzyme deactivation, and filtering enzymatic hydrolysate to obtain supernate; and (3) carrying out spray drying on the supernate, and collecting dried powder, namely the eggshell membrane powder. The preparation method solves the problems of complicated operation, chemical reagent residues, high difficulty in multi-enzyme synergistic regulation and the like in the existing preparation technology of the eggshell membrane powder, and the content of active ingredients such as collagen, hyaluronic acid and chondroitin sulfate in the prepared eggshell membrane powder is equivalent to that in the prior art; and the method has the outstanding advantages of simplified process, greenness, safety and controllable cost.

The application discloses a preparation method of a gastrointestinal inflammation treating tablet, and steps of the method comprise the following steps: (1) preparing an extract solution of lotus leaves and red dates to obtain extract solution I; preparing an extract solution of eggshell membrane powder to obtain extract solution II; combining the extract solution I and the extract solution II, performing enzyme inactivation treatment, and concentrating the combined solution to a paste under reduced pressure to obtain an extract paste; (2) adding pueraria powder, threonine and tryptophan into the extract paste, and uniformly stirring the mixture after the addition is completed to obtain an auxiliary material; and (3) preparing a paste body, adding the auxiliary material, compressing the paste body into a tablet, and coating the tablet with sugar or film coating to obtain the tablet. The auxiliary material prepared by the method has good bacteriostasis, and has good inhibition effect on escherichia coli and staphylococcus aureus; after being added into the tablet, the auxiliary material can effectively improve the bactericidal and bacteriostatic effect of the tablet; meanwhile, the prepared tablet has low friability, so that the tablet is not prone to powder dropping, fragmentation and other phenomena during packaging, transportation and processing, and the qualified rate of the finished product is high.

The invention discloses a pet cat food formula and a making process thereof. The pet cat food formula comprises animal basic components: 25% of enzymolysis fresh chicken with bones, 30% of fresh chicken, 10% of fresh complete quail, 8% of fish scale-containing fresh salmon, 5% of fresh chicken liver, 4% of fresh chicken heart and 3% of fermented animal oil; the functional additives comprise 6% of functional fruit and vegetable groups, 2% of freeze-dried queen bee larva powder, 0.5% of propolis, 0.5% of wall-broken bee pollen, 2% of euphausia superba powder, 1% of ostrich liver powder, 1% of catgrass powder and 0.5% of eggshell membrane powder; nutritional additives: 2% of microencapsulated egg yolk powder and 0.2% of a probiotic post-spraying bag; and 0.3% of montmorillonite. According to the method, the meat with bones is pretreated through an enzymolysis technology, macromolecular protein and calcium are pre-decomposed, and the protein digestibility and the calcium bioavailability are remarkably improved; the fermented animal oil is decomposed into smaller molecules and can be directly absorbed without bile emulsification, so that the fat absorption rate is higher; the microencapsulation technology protects the activity of thermosensitive nutrients, and the overall nutrition utilization efficiency is greatly improved.

The present invention relates to a composition comprising a peptide fraction, wherein: the peptide fraction comprises cysteine and methionine; the mass content of methionine of the peptide fraction is greater than 1% relative to the total amino acids of the peptide fraction; the cysteine of the peptide fraction is at least partially sulfonated, the degree of sulfonation of the cysteine being greater than 50%, preferably greater than 70%, and even more preferably greater than 80%.

The present invention relates to a composition and method for solubilizing eggshell membrane at convenient rates. The present invention also relates to organoleptically acceptable products obtained therein, and to their oral or topical application to improve a disease or condition in a subject.

The application belongs to the technical field of feed additives, and provides a mixed feed additive for improving immunity of an animal body and a preparation method thereof.The mixed feed additive for improving immunity of an animal body comprises the following components in parts by weight: 20-25 parts of enzymolysis eggshell membrane powder, 38-42 parts of enzymolysis bamboo fiber, 0.5-0.8 parts of probiotics, 10-15 parts of beet powder, 8-12 parts of psyllium powder and 4-6 parts of dandelion powder.The application can effectively enhance the immunity of the animal body.

The present application relates to the technical field of all-solid-state lithium metal battery, and particularly relates to a solid-state polymer electrolyte and a preparation method and application thereof. In the present application, trifluoroethanol is used to fluorinate eggshell membrane powder in natural eggshell, which is used as a polyethylene oxide-based solid-state polymer electrolyte additive. This can adjust the network structure of the polymer, enhance the electrochemical and mechanical performance of the solid-state electrolyte, inhibit lithium dendrite growth, and enhance the cycle performance of the lithium metal battery. The lithium ion transfer number of the improved solid-state electrolyte battery is determined by chronamperometry, and the lithium ion transfer number is higher, and it is easier to generate fluorinated lithium and lithium oxide, and better inhibit the growth of lithium dendrites. The addition of trifluoroethanol to fluorinate the eggshell membrane solid-state electrolyte in the natural eggshell makes the lithium symmetrical battery and the lithium iron phosphate full battery exhibit excellent performance in various cases.

The invention provides a method for improving emulsification characteristics of eggshell membrane peptide emulsion by combined utilization of phosphorylation modification and polyphenol composite technologies. The method comprises the following steps: taking eggshell membrane powder, sodium trimetaphosphate, procyanidine and the like as preparation raw and auxiliary materials, performing enzymolysis, centrifugation, phosphorylation reaction, ultrafiltration and freeze drying to prepare phosphorylated eggshell membrane active peptide, then mixing the phosphorylated eggshell membrane active peptide with procyanidine in equal proportion, reacting in a shaking table at room temperature for 1 hour to form an aggregate solution, and drying the aggregate solution to obtain the product. And mixing with medium chain triglyceride in proportion, and carrying out high-speed homogenization and ultrasonic treatment to obtain the stable emulsion. The prepared phosphorylated eggshell membrane peptide-procyanidine compound aggregate emulsion has good emulsifying performance and storage stability, and the application range of the eggshell membrane peptide in the food field is widened.

The application discloses an eggshell membrane steam explosion pretreatment device and method and the technical field of steam explosion, and comprises a steam explosion tank, a high-pressure steam supplier, a rapid pressure relief device, a mechanical crushing device, a pre-crushing module and a separation screening module. The application loosens the material in advance through the double-toothed roll crusher of the pre-crushing module, uses high-pressure steam explosion to combine the rapid pressure relief device with extremely fast response to generate a high-speed airflow to tear the eggshell membrane, and uses the pressure relief airflow to drive the built-in radial flow impeller to rotate at high speed, the transmission shaft drives the multi-layer staggered cutter head with a high-hardness cubic boron nitride coating, and cooperates with the fixed stepped tooth plate to realize efficient recycling of the pressure relief energy under the stability of the speed-regulating flywheel, significantly reduces external energy consumption, and integrates the pre-crushing, steam explosion, energy recovery crushing and subsequent separation screening modules into one, greatly improves the efficiency, automation degree and final product yield and quality of the eggshell membrane separation treatment.

The invention discloses an eggshell and shell membrane efficient smashing and separating device and method, and relates to the technical field of eggshell treatment.The eggshell and shell membrane efficient smashing and separating device comprises a base, a separating mechanism and a discharging mechanism, a separating tank is arranged on the base, an eggshell membrane collecting tank is arranged on the upper side of the separating tank, and the separating mechanism is arranged in the separating tank on the base; the separation mechanism comprises bearing seats and a separation barrel, first rotating rods are fixedly connected to the two sides of the separation barrel, the bearing seats are symmetrically arranged in the separation tank, the first rotating rods are located on the bearing seats, and a discharging mechanism is arranged on one side of the separation tank. Meanwhile, a second rotating rod drives a turbulent flow piece on a rotating wheel to generate turbulent flow on the eggshells, auger blades whip the eggshells to achieve preliminary separation of shell membranes, coarsely broken eggshells fall into the lower layer through a filter screen to be collected, positive pressure air enters through a fan and is blown out upwards through the filter screen, and the eggshell membranes are prevented from being mixed into the eggshells.

The present invention discloses a type X collagen peptide for improving bone growth and its preparation method and application, relating to the field of biotechnology. The preparation method comprises the following steps: S1, eggshell membrane powder is extracted with water to obtain a crude extract; S2, enzyme is added to the crude extract for enzymolysis to obtain an enzymolysis solution; S3, natto Bacillus powder is inoculated in the enzymolysis solution, the inoculation amount is 0.05-1%, the temperature is 38-45 ° C, and the fermentation is 14-36h; the temperature is lowered to 25-30 ° C, lactic acid Kluyveromyces is inoculated, the inoculation amount is 1-6%, and the fermentation is 3-7 days to obtain eggshell membrane enzymatic fermentation liquid; S4, the eggshell membrane enzymatic fermentation liquid is centrifuged, a clarifier is added to obtain a clarified liquid; S5, a decolorizing agent is added to the clarified liquid, and it is dried to obtain a type X collagen peptide. The collagen peptide of the present invention has a high content of type X collagen, which can promote osteoblast growth and has broad application prospects in the preparation of growth-promoting products.

The application belongs to the field of biomedical materials and biomedical engineering technology, and discloses a photothermal responsive curcumin sustained-release microgel as well as a preparation method and application thereof. The preparation method is as follows: eggshell membrane short fibers are soaked in a dopamine aqueous solution, centrifuged and washed after dark storage, and dopamine-eggshell membrane short fibers are obtained; the dopamine-eggshell membrane short fibers, curcumin and sodium alginate are mixed, dropped into a calcium chloride solution for shaping, and a photothermal responsive curcumin sustained-release microgel is obtained. The microgel has excellent water content, compact microstructure, biocompatibility, blood compatibility and photothermal response function, and can be applied to precise and controllable drug loading.

The invention provides a membrane phospholipid composition specific liposome for promoting eggshell mineralization and application thereof. The liposome membrane is prepared from the following components as raw materials: (a) core membrane phospholipid; (b) an acidic phospholipid and (c) a membrane stabilizing component; wherein the core membrane phospholipid comprises PC (Polycarbonate) and PE (Polyethylene); the acidic phospholipid is PS and / or PA; the membrane stabilizing component comprises at least one of methoxy polyethylene glycol modified phosphatidyl ethanolamine (DSPE-PEG), 1, 2-dimyristoyl-rac-glycerol-3-methoxy polyethylene glycol (DMG-PEG), 1, 2-dioleoyl-sn-glycerol-3-phosphoethanolamine-N-[methoxy (polyethylene glycol)] (DOPE-PEG), and cholesterol (Cholesterol). Through specific combination of core membrane phospholipids (PC and PE) and acidic phospholipids (PS and / or PA), an initial mineralization microenvironment in a living body is successfully simulated. In-vitro mineralization test results show that the liposome can effectively promote formation and growth of calcium carbonate crystal nucleuses, has excellent performance on interfaces of eggshell membranes, silicon wafers and the like, and proves universality and effectiveness.

A composition which is a hydrogel including, in an aqueous medium: at least one hydrophilic thickening polymer, and particles of eggshell membrane, in an amount of less than 5% by weight of the total weight of the composition, wherein the particles are rod-shaped, needle-shaped or fibrous, having a specific particle size. Also described is a bioink including the composition, a method for manufacturing a 2D or 3D product using the hydrogel, a kit including the hydrogel or bioink, and to uses thereof.

The invention discloses a poultry eggshell membrane steam explosion pretreatment device and method, and relates to the technical field of steam explosion. The poultry eggshell membrane steam explosion pretreatment device comprises a steam explosion tank, a high-pressure steam supplier, a rapid pressure relief device, a mechanical crushing device, a pre-crushing module and a separating and screening module. Materials are loosened in advance through a double-geared-roller crusher of the pre-crushing module, high-pressure steam explosion is combined with a quick pressure relief device which is extremely quick in response to generate instantaneous high-speed airflow to tear eggshell membranes, and pressure relief airflow is used for driving a built-in radial flow type impeller to rotate at a high speed; the transmission shaft drives the multiple layers of staggered cutterheads with high-hardness cubic boron nitride coatings and cooperates with the fixed stepped toothed plate, under the condition that the speed regulation flywheel is stable, efficient recycling of pressure relief energy is achieved, external energy consumption is remarkably reduced, meanwhile, a pre-crushing module, a steam explosion module, an energy recycling and crushing module and a subsequent separation and screening module are integrated, and the production efficiency is improved. The efficiency and the automation degree of eggshell membrane separation treatment as well as the yield and the quality of final products are greatly improved.

To provide a food freshness sensor capable of easily determining the freshness of food using an eggshell membrane or a material derived from the eggshell membrane.SOLUTION: A food freshness sensor comprises a color-changing pigment which changes color in response to substances emitted in association with food spoilage and an eggshell membrane which adsorbs the color-changing pigment or a material derived from the eggshell membrane. Polyaniline or curcumin can be suitably used as the color-changing pigment. The eggshell membrane is an inexpensive, environmentally friendly, and sustainable material. Such a food freshness sensor including the eggshell membrane and the color-changing pigment improves safety related to food freshness and quality, helps reduce food waste, and contributes to the improvement of food supply and society.SELECTED DRAWING: Figure 26

The invention relates to the technical field of pet food processing, and particularly provides a protein-lipid compound oil gel coating composition which comprises the following components in parts by weight: 60-70 parts of egg yolk oil; 10 to 20 parts of eggshell membrane peptide; 5-10 parts of medium-chain fatty acid; 0.1 to 0.5 part of vitamin E; 10 to 15 parts of ethyl cellulose; and 2-5 parts of nano silicon dioxide. Egg oil is used as a substrate, an oil gel three-dimensional network is constructed, eggshell membrane protein is integrated into lipid, and precise function delivery of joint and fur health is achieved. The solid coating is stable in structure and high in oxidation resistance, can be firmly attached to the surface of the pet food, effectively prevents grease oxidation and leakage, and remarkably prolongs the shelf life and improves the additional value of the product.