273 results about "Polypeptide composition" patented technology

The invention relates to the field of polypeptide compositions, in particular to a polypeptide metal chelate and application thereof in the field of cosmetics, and the polypeptide metal chelate is formed by chelating a polypeptide compound and a metal ion compound. The structure of the polypeptide compound is [gamma-Glu] n-AA, n is greater than or equal to 1, and AA is selected from glycine, alanine, valine, leucine, isoleucine, methionine (methionine), proline, tryptophan, serine, tyrosine, cysteine, phenylalanine, asparagine, glutamine, threonine, asparaginic acid, glutamic acid, lysine, arginine or histidine; the polypeptide chelated zinc prepared by the invention has remarkable oil control and anti-inflammatory effects, and can be compounded with other raw materials to form cosmetics with oil control and acne removal effects.

Disclosed herein are molecules and pharmaceutical compositions that induce an insertion, deletion, duplication, or alteration in an incorrectly spliced mRNA transcript to induce exon skipping or exon inclusion. Also described herein include methods for treating a disease or disorder that comprises a molecule or a pharmaceutical composition that induces an insertion, deletion, duplication, or alteration in an incorrectly spliced mRNA transcript to induce exon skipping or exon inclusion.

Disclosed herein are molecules and pharmaceutical compositions that induce an insertion, deletion, duplication, or alteration in an incorrectly spliced mRNA transcript to induce exon skipping or exon inclusion. Also described herein include methods for treating a disease or disorder that comprises a molecule or a pharmaceutical composition that induces an insertion, deletion, duplication, or alteration in an incorrectly spliced mRNA transcript to induce exon skipping or exon inclusion.

The invention provides a preparation method and application of a camellia polypeptide composition with anti-wrinkle, repairing and brightening effects. The camellia polypeptide composition comprises a component A and a component B, wherein the component A comprises a wrapping solubilizer and polyhydric alcohol; component B: a plurality of polypeptides; the component C comprises carnosine, camellia polypeptide and water. The camellia polypeptide disclosed by the invention is prepared by the following steps: performing supercritical deoiling on camellia seeds, performing alkali extraction, acid precipitation and enzymolysis to prepare polypeptide, performing membrane filtration, taking micromolecule polypeptide as a main component, promoting synthesis of type I, type III and type V collagen, promoting cell migration and scavenging free radicals, and has the effects of resisting wrinkles, repairing and resisting oxidation; according to the invention, the problem of water solubility of palmitoyl peptide and acetyl peptide is solved, so that palmitoyl peptide and acetyl peptide can form a stable composition together with water-soluble polypeptide. The anti-wrinkle, repairing and whitening effects are achieved in multiple ways and multiple directions, the effects of improving wrinkles, repairing the skin, brightening the skin color and delaying senescence can be better achieved, and the anti-wrinkle, repairing and whitening composition can be widely applied to various types of cosmetics.

The invention discloses a screening method of hericium erinaceus functional polypeptide based on artificial intelligence assistance and polypeptide or a polypeptide composition. The method comprises the following steps: (1) constructing an AI auxiliary annotation model; (2) extracting hericium erinaceus crude protein; (3) preparing hericium erinaceus protein enzymatic hydrolysate through composite enzymatic hydrolysis; (4) performing polypeptide identification on the hericium erinaceus protein enzymatic hydrolysate to obtain a polypeptide sequence; and (5) carrying out AI auxiliary annotation and polypeptide screening by using a large language model based on a DeepSeek platform so as to obtain a polypeptide sequence with assumed biological activity. The polypeptide sequence and the polypeptide composition which have remarkable ACE inhibition and oxidation resistance dual functions are successfully obtained by utilizing the method disclosed by the invention. Compared with a traditional experience screening path, the method realizes a high-throughput active peptide development process which is targeted in structure, accurate in prediction and capable of verifying a closed loop.

Disclosed herein are molecules and pharmaceutical compositions that induce an insertion, deletion, duplication, or alteration in an incorrectly spliced mRNA transcript to induce exon skipping or exon inclusion. Also described herein include methods for treating a disease or disorder that comprises a molecule or a pharmaceutical composition that induces an insertion, deletion, duplication, or alteration in an incorrectly spliced mRNA transcript to induce exon skipping or exon inclusion.

The invention belongs to the technical field of biological medicine, and relates to a composite polypeptide composition as well as a preparation method and application thereof. According to the technical scheme, pH responsive polymer loaded oligopeptide-1, acetyl heptapeptide-4 and palmitoyl tripeptide-1 are adopted as a core, hyaluronic acid is adopted for wrapping, a hyaluronic acid layer adsorbs palmitoyl tripeptide-8, heptapeptide-6 and decapeptide-4, and the composite polypeptide with a core-shell structure is prepared and used for treating inflammatory skin diseases. In the acute stage of inflammation, the active ingredients on the outer layer quickly inhibit the activation of proinflammatory factors and immune cells and relieve red, swollen and heat pain, and the gel outer layer formed by hyaluronic acid realizes continuous release, prolongs the action time and supplements moisture to the skin; the pH-responsive polymer of the inner layer releases active ingredients at the inflammation part, so that relapse is prevented, and the skin barrier function is repaired. The composition plays a role from multiple dimensions of inflammation regulation, barrier repair, oxidation resistance and the like through a multi-layer structure design and a multi-component synergistic effect.

The invention provides a polypeptide composition and application thereof in treating male erectile dysfunction. Specifically, the polypeptide composition contains the erectile peptide screened and identified by the invention, and the erectile peptide has good oxidation fatigue resistance, can effectively promote penile erection, improve testosterone level and effectively treat erectile dysfunction, and has wide application prospects.

The present invention is directed to an artificial nucleic acid and to polypeptides suitable for use in treatment or prophylaxis of an infection with Norovirus or a disorder related to such an infection. In particular, the present invention concerns a Norovirus vaccine. The present invention is directed to an artificial nucleic acid, polypeptides, compositions and vaccines comprising the artificial nucleic acid or the polypeptides. The invention further concerns a method of treating or preventing a disorder or a disease, first and second medical uses of the artificial nucleic acid, polypeptides, compositions and vaccines. Further, the invention is directed to a kit, particularly to a kit of parts, comprising the artificial nucleic acid, polypeptides, compositions and vaccines.

The invention belongs to the field of biological medicines, particularly relates to an oral polypeptide composition system capable of being stably absorbed in gastrointestinal tracts, and particularly discloses an oral polypeptide composition which comprises polypeptide molecules (A), a surfactant (B) and an amino acid single-molecule or double-molecule combination (C), the molecular weight of the peptide medicine used as the medicine in the oral polypeptide composition is in the range of 0.1 kDa to 20 kDa. The acting part of the composition system is small intestine or large intestine; the stability and absorbability of the polypeptide composition in gastrointestinal tracts are obviously improved and enhanced.

The invention discloses application of a broad-spectrum influenza virus polypeptide vaccine in preparation of a medicine for preventing influenza virus infection and controlling influenza virus reinfection, and belongs to the technical field of biological medicine. The vaccine is designed on the basis of artificially synthesized polypeptide, a polypeptide sequence which is highly conservative and has immunocompetence is screened and optimized aiming at various subtype influenza virus proteins, a broad-spectrum polypeptide vaccine P125-H is constructed, and an Ii-Key technology and an MF59 adjuvant are applied to enhance immune response. The vaccine can effectively induce immune protection against H1N1 and H7N9 influenza A viruses, significantly reduce the virus load, reduce pathological injury to the lung and improve the survival rate. The P125-H vaccine is composed of three optimized polypeptides, has a cross protection effect on various influenza virus subtypes, has the potential of being developed into a broad-spectrum influenza vaccine, and provides a new technical scheme for coping with influenza virus variation and improving vaccine efficacy.

The invention discloses a composition containing probiotics and fusion polypeptide and application of the composition in treatment of intestinal inflammatory diseases. The composition is prepared from lactobacillus plantarum (Lactobacillus plantarum CCFM8661) and a functional fusion polypeptide, wherein the amino acid sequence of the fusion polypeptide is as shown in SEQ ID NO. 1. The invention also discloses a preparation method of the composition. The fusion polypeptide can be used for directly inhibiting the growth of pathogenic bacteria and neutralizing toxins by enhancing the intestinal colonization capability of probiotics, so that the triple synergistic effect of bacteriostasis, repair and immunoregulation is realized. Experiments show that the composition significantly reduces the disease activity index in a DSS-induced mouse colitis model, recovers the colon length, and inhibits serum inflammatory factors IL-6 and TNF-alpha. In addition, the survival rate of the freeze-dried microcapsule preparation in simulated gastric juice is gt; the colon targeted release characteristic is realized. The invention provides an efficient and stable new treatment strategy for intestinal inflammatory diseases.

The invention relates to novel bispecific antibodies consisting of two fusion polypeptides comprising two antigen binding domains capable of specific binding to a first target and one antigen binding domain capable of specific binding to a second target, and to methods of producing these molecules and to methods of using the same.

The present application provides a vaccine comprising: 1) a peptide oligomer consisting of 2-8 copies of a bacteriophage T4 fibrin C-terminal polypeptide and one or more antigen proteins; or 2) a coding nucleic acid of item 1) wherein 2-8 copies of the bacteriophage T4 fibrin C-terminal polypeptides in the peptide oligomer are directly linked in series through chemical bonds or each of the bacteriophage T4 fibrin C-terminal polypeptides is linked to each other through a peptide linker, and the peptide oligomer and the antigen protein are directly connected through a chemical bond or connected through a peptide linker. In the bi-T4-virus antigen vaccine platform provided by the invention, the antigen can be subjected to multi-component change, that is, the antigen can form a trimer, so that the use flexibility of the vaccine platform is enhanced, the application range of the vaccine platform is widened, and the bi-T4 structure can enhance the formation of trimer protein and enhance the immune aging.

The invention discloses a fermented caffeine eye cream composition. Comprising the following components in parts by weight: 30 to 80 parts of a fermentation composition, 1 to 5 parts of caffeine, 0.1 to 0.3 part of hydroxypropyl tetrahydropyrantriol, 0.5 to 2 parts of nicotinamide, 0.1 to 1 part of squalane, 0.01 to 0.5 part of a ceramide composition, 0.005 to 0.05 part of dipeptide diaminobutyryl benzyl amide diacetate, 0.005 to 0.05 part of a polypeptide composition, 0.01 to 0.5 part of carnosine, 0.01 to 0.5 part of tetrahydromethylpyrimidine carboxylic acid and 3 to 12 parts of a plant extract. The invention also discloses an eye cream containing the eye cream composition. The fermented caffeine eye cream composition provided by the invention can effectively smooth the skin surface, and is beneficial to preventing and comforting aging, improving skin elasticity, improving dark circles, relieving edema and removing under-eye puffiness.

The invention relates to the field of biological medicine, in particular to a polypeptide composition with bacteriostatic activity and a preparation method thereof.The polypeptide composition is prepared by wrapping polypeptide I with modified polyethylene glycol, then wrapping with nano-silver and finally modifying polypeptide II with the nano-silver. The outer layer is sequentially wrapped with spherical particles of modified polyethylene glycol, nano-silver and polypeptide II, and the polypeptide II and the nano-silver are promoted to be combined with a bacterial membrane and destroy the bacterial membrane through the non-specific electrostatic interaction of the polypeptide II and the nano-silver, so that the polypeptide II has relatively high antibacterial performance; the outer-layer polypeptide is degraded by proteolytic enzyme or plays a role with a bacterial membrane, so that the inner-layer polypeptide I wrapped by the modified polyethylene glycol is exposed, the antibacterial effect is further played, and the proteolytic stability of the polypeptide is improved; due to the spherical structure, the prepared polypeptide composition with the antibacterial activity has excellent antibacterial performance, meanwhile, the stability can be improved, and the polypeptide composition has wide application prospects.

The invention belongs to the field of cosmetics, and discloses a ceramide E-containing polypeptide composition and application thereof. The polypeptide composition containing ceramide E is prepared from the following components in parts by mass: 5 to 10 parts of ceramide E, 3 to 6 parts of fibronectin, 3 to 6 parts of palmitoyl pentapeptide-4, 1.5 to 3.5 parts of ergothioneine, 20 to 40 parts of oil-phase solvent, 1.5 to 4.5 parts of caprylic / capric triglyceride, 0.5 to 2.5 parts of soybean lecithin, 0.5 to 1.5 parts of polysorbate-80, 0.1 to 0.5 part of cholesterol and the balance of water, wherein the total mass part of the water is 100. In the polypeptide composition disclosed by the invention, ceramide E, fibronectin, ergothioneine and palmitoyl pentapeptide-4 are mutually promoted and have synergistic interaction, and a final product shows very good repairing and antioxidant effects.

The invention discloses a composite plant polypeptide composition with the effects of repairing pancreas and reducing blood sugar. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10 to 50 parts of ginsenoside, 5 to 30 parts of radix astragali seu hedysari extract, 3 to 20 parts of radix rehmanniae polysaccharide, 2 to 15 parts of radix trichosanthis alkaloid, 5 to 25 parts of rhizoma dioscoreae polysaccharide, 5 to 30 parts of bitter gourd polypeptide, 3 to 20 parts of folium mori alkaloid, 8 to 40 parts of rhizoma polygonati polysaccharide, 5 to 25 parts of fructus hippophae flavone, 10 to 35 parts of wheat germ peptide, 2 to 15 parts of coptis berberine, 10 to 45 parts of corn stigma polysaccharide and 3 to 20 parts of saussurea involucrata polyphenol. 1-10 parts of sophora flavescens total alkaloids, 5-25 parts of a dendrobium extract, 5-30 parts of a radix puerariae extract, 8-35 parts of L-arabinose and 10-40 parts of small molecule peptides; 20 to 100 parts of collagen peptide and 15 to 80 parts of albumin peptide; 5-50 parts of microcrystalline cellulose, 3-30 parts of fructo-oligosaccharide and 0.5-5 parts of magnesium stearate; the limitation of single sugar control or simple component mixing in the prior art is broken through, a synergistic system of plant active components and functional polypeptides is constructed, and the purity and retention rate of the plant active components are improved by optimizing the extraction process.

The invention discloses a moisturizing anti-wrinkle polypeptide composition as well as a preparation method and application thereof, and relates to the technical field of daily chemical materials. The invention discloses a preparation method of a moisturizing and anti-wrinkle polypeptide composition, which comprises the following steps: uniformly stirring meadowfoam seed oil, caprylic acid / capric acid / succinic triglyceride and polypeptide liposome to obtain the moisturizing and anti-wrinkle polypeptide composition, wherein the polypeptide liposome is prepared by taking a combination of soybean lecithin, modified cholesterol and cholesterol as a capsule material and taking acetyl hexapeptide-8, snake venom peptide and a blue copper peptide compound as a core material through an ethanol injection method. The modified cholesterol is obtained by modifying cholesterol, the modified cholesterol is added into the capsule material, the defects that an existing liposome material is prone to aggregation and leakage, poor in stability and the like are effectively overcome, and the prepared material is good in stability, capable of being slowly released, high in skin retention amount and capable of being more easily absorbed by skin cells; the function of obviously delaying skin aging is realized.

The invention relates to the technical field of molecular probes, and particularly discloses a molecular probe of a targeted tyrosine kinase receptor as well as a preparation method and application of the molecular probe, the molecular probe comprises radioisotope, targeted polypeptide and a chelating group, the targeted polypeptide specifically targets the tyrosine kinase receptor, the chelating group is connected with the targeted polypeptide to form a targeted precursor, and the chelating group is connected with the targeted polypeptide to form the targeted precursor. The radioactive isotope labeled precursor forms a molecular probe, and the sequence of the targeted polypeptide is shown as any one of SEQ ID No.1-8. The targeting polypeptide has good targeting property on a tyrosine kinase receptor coded by an RET gene, can realize specific high uptake in a cell line of NEPC, has relatively good biological safety, can realize accurate imaging aiming at NEPC, and clearly and durably visualizes NEPC tumors in PET imaging; meanwhile, the molecular probe can effectively delay the growth capacity of NEPC, can provide a new treatment choice for NEPC patients, and has good clinical transformation potential.

Provided herein are polypeptide compositions and chimeric antigen receptors comprising natural ligands of hematopoietic cell receptors and are thereby able to specifically bind to hematopoietic cell receptors. Also provided are methods and compositions for using the polypeptide compositions and chimeric antigen receptors in the treatment of disease, such as cancer, for editing a gene in a cell, and for culturing populations of cells.

The invention discloses a preparation method of a polypeptide composition with anti-wrinkle and firming effects, and belongs to the technical field of cosmetic raw material preparation, and the preparation method comprises the following steps: S1, raw material pretreatment; s2, mixing the raw materials; s3, adjusting the pH value: adjusting the pH value of the polypeptide mixed solution to 5.5-6.5 by using a hydrophosphate or citric acid solution; s4, filtering and degerming: filtering and degerming the polypeptide mixed solution of which the pH value is adjusted through a 0.22 mu m microporous filter membrane to obtain a sterile polypeptide composition solution; and S5, filling: filling the sterile polypeptide composition solution into a proper packaging container. Through synergistic compatibility of five polypeptides such as palmitoyl pentapeptide-3 and acetyl hexapeptide-8, the anti-wrinkle and firming effects are achieved from four mechanisms of stimulating collagen regeneration, inhibiting neural signals, blocking protease degradation and resisting oxidation.

The invention discloses application of SMCP in preparation of a rheumatoid arthritis diagnosis product, an anti-SMCP antibody in a biological sample of a patient is used as a marker, and the diagnosis product is used for detecting the content of the anti-SMCP antibody. The SMCP is a polypeptide mixture which is prepared from citrullinated SR-A polypeptide, citrullinated MCV polypeptide, citrullinated caspase14 polypeptide and citrullinated PCOLCE polypeptide. According to the present invention, the anti-SMCP antibody can be subjected to one-time detection so as to achieve the accurate diagnosis of rheumatoid arthritis (RA); besides, the anti-SMCP antibody also has better auxiliary diagnostic value for RA patients with negative anti-CCP antibody, and when the anti-SMCP antibody and the anti-CCP antibody are combined for diagnosis, the RA detection rate can be further improved under the condition of keeping high specificity, and the targets of early diagnosis, early treatment and reduction of disability rate can be more favorably realized.

The present invention relates to activatable recombinant polypeptide compositions comprising a cleavage release segment. In some instances, the activatable recombinant polypeptide compositions include an XTEN linked to binding moieties by cleavable release segments that, when cleaved, the binding moieties are capable of binding together effector T cells with targeted tumor or cancer cells and effecting cytolysis of the tumor cells or cancer cells. The invention also provides compositions and methods of making and using the cleavable activatable recombinant compositions.

The application discloses a polypeptide composition for treating obesity and related metabolic diseases and application thereof. The polypeptide composition comprises polypeptide 1 and polypeptide 2, wherein the amino acid sequence of the polypeptide 1 is shown as SEQ ID NO. 1, can be specifically combined with the CD105 receptor on the surface of MSCs and the AdipoR1 receptor of adipocytes; the amino acid sequence of the polypeptide 2 is shown as SEQ ID NO. 2, can target adipose tissue and regulate glycolipid metabolism. The polypeptide composition is combined with fat-derived mesenchymal stem cells (MSCs) and applied to a high-fat-diet obese mouse model, and the results show that the composition has a significant synergistic effect in reducing body fat, improving the morphology of adipose tissue, regulating serum metabolic factors and inflammatory factors, and has no obvious organ toxicity. The application provides a new effective strategy and a safe and reliable pharmaceutical composition for treating obesity and related metabolic diseases.

The invention discloses an antioxidant and firming polypeptide composition and a preparation method thereof, and the antioxidant and firming polypeptide composition comprises the following components in percentage by weight: 50-75% of water, 5-10% of butanediol, 0.1-3% of panthenol and 0.5-1% of p-hydroxyacetophenone. 0.2 to 8% of hydroxyethyl piperazine ethanesulfonic acid; the composition is prepared from the following components in percentage by weight: 20-30% of an eggshell membrane extract, 0.01-2% of nonapeptide-1, 0.01-5% of acetyl hexapeptide-8 and 0.5-1% of 1, 2-hexanediol; the preparation method comprises the following steps: putting water, butanediol, panthenol and p-hydroxyacetophenone into a container, and stirring at a constant temperature to obtain a first mixed solution; adding hydroxyethyl piperazine ethanesulfonic acid into the first mixed solution, and uniformly stirring to obtain a second mixed solution; adding an eggshell membrane extract, nonapeptide-1, acetyl hexapeptide-8 and 1, 2-hexanediol into the second mixed solution, and uniformly stirring to obtain the antioxidant and firming polypeptide composition; the antioxidant and firming polypeptide composition disclosed by the invention has the functions of resisting oxidation, firming and brightening the skin; the method for preparing the antioxidant and firming polypeptide composition is simple and convenient for large-scale production.

The present application relates to a kind of hydrolysis targeting chimera based on artificial antibody and its preparation method and application.The hydrolysis targeting chimera is composed of artificial gold antibody and molecule that can activate cell degradation mechanism, the artificial gold antibody is composed of gold nanoparticle and polypeptide coupled on the surface of gold nanoparticle, the molecule that can activate cell degradation mechanism is coupled on the surface of gold nanoparticle.The hydrolysis targeting chimera can simultaneously bind target protein and activate cell degradation mechanism, with the performance of strong specific recognition target protein, and high stability, orientation controllable.At the same time, the hydrolysis targeting chimera can also play the molecule of activation cell degradation mechanism, such as lysosome sorting signal motif, coupled on the surface, induce clathrin-mediated endocytosis, transport the complex of target protein and chimera to lysosome and degrade.The hydrolysis targeting chimera can play multi-mode cancer treatment effect by synergistic inhibition of tumor cell proliferation, invasion pathway and apoptosis activation.

The invention discloses an EGFR (epidermal growth factor receptor) targeting artificial antibody based on gold nanoparticles and a preparation method of the EGFR targeting artificial antibody. The artificial antibody is composed of gold nanoparticles and unstructured polypeptide coupled with the surfaces of the gold nanoparticles. The nanogold artificial antibody is prepared by reconstructing the'dimerization arm 'conformation of the polypeptide on the surfaces of the gold nanoparticles. The artificial antibody has the capability of specifically recognizing EGFR, and is strong in binding force and good in stability. Meanwhile, the compound can be specifically combined with EGFR overexpressed cancer cells, so that proliferation of the cancer cells is inhibited, and death of the cancer cells is induced. Therefore, the artificial antibody with the function of specifically recognizing the EGFR has a wide application prospect in the fields of disease diagnosis, targeted therapy, photo-thermal therapy, drug carrying and the like.

The invention discloses a polypeptide composition for promoting skin desquamation and a preparation method and application thereof, the polypeptide composition comprises polypeptides and a polyol preservative, the polypeptides comprise a first polypeptide, a second polypeptide and a third polypeptide, and the first polypeptide is acetyl tetrapeptide-2; the second polypeptide comprises one or more of hyaluronic acid polypeptide, red scorpion toxin and palmitoyl tripeptide-5; and the third polypeptide is prepared from one or more of acetyl octapeptide-3, palmitoyl dipeptide-7, acetyl tetrapeptide-9 and acetyl tetrapeptide-11. The polypeptide composition can significantly up-regulate the gene expression of kallikrein through a synergistic effect and promote old cutin exfoliation; meanwhile, gene expression of metalloproteinase is inhibited, and light aging is delayed. The polypeptide composition disclosed by the invention can be prepared into various cosmetics and has multiple effects of promoting skin desquamation, resisting wrinkles and repairing barriers.

Described herein are method of using fusion proteins composed of elements such as one or more proteins / polypeptides capable of translocating into an endoplasmic reticulum (ER) and an interleukin 18 (IL-18) or a fragment or variant of IL-18. The proteins / polypeptides capable of translocating into the ER include but are not limited to an immunoglobular protein such as Fc region, VHH antibody, and scFv, and a globular protein such as serum albumin. The IL-18 may be a precursor IL-18 that resembles the natural pro-cytokine in which IL-18 is fused to the C-terminus of its propeptide or a propeptide variant. These fusion proteins can be used in methods of treating cancer or fibrosis by activating an IL-18 receptor complex (RC) signal on a cell, releasing or exposing mature IL-18 in a tumor microenvironment or adjacent to a tumor and / or increasing memory CD8+ T cell proliferation.