96 results about "Polypeptide composition" patented technology

The invention discloses a screening method of hericium erinaceus functional polypeptide based on artificial intelligence assistance and polypeptide or a polypeptide composition. The method comprises the following steps: (1) constructing an AI auxiliary annotation model; (2) extracting hericium erinaceus crude protein; (3) preparing hericium erinaceus protein enzymatic hydrolysate through composite enzymatic hydrolysis; (4) performing polypeptide identification on the hericium erinaceus protein enzymatic hydrolysate to obtain a polypeptide sequence; and (5) carrying out AI auxiliary annotation and polypeptide screening by using a large language model based on a DeepSeek platform so as to obtain a polypeptide sequence with assumed biological activity. The polypeptide sequence and the polypeptide composition which have remarkable ACE inhibition and oxidation resistance dual functions are successfully obtained by utilizing the method disclosed by the invention. Compared with a traditional experience screening path, the method realizes a high-throughput active peptide development process which is targeted in structure, accurate in prediction and capable of verifying a closed loop.

The application discloses a polypeptide composition for treating obesity and related metabolic diseases and application thereof. The polypeptide composition comprises polypeptide 1 and polypeptide 2, wherein the amino acid sequence of the polypeptide 1 is shown as SEQ ID NO. 1, can be specifically combined with the CD105 receptor on the surface of MSCs and the AdipoR1 receptor of adipocytes; the amino acid sequence of the polypeptide 2 is shown as SEQ ID NO. 2, can target adipose tissue and regulate glycolipid metabolism. The polypeptide composition is combined with fat-derived mesenchymal stem cells (MSCs) and applied to a high-fat-diet obese mouse model, and the results show that the composition has a significant synergistic effect in reducing body fat, improving the morphology of adipose tissue, regulating serum metabolic factors and inflammatory factors, and has no obvious organ toxicity. The application provides a new effective strategy and a safe and reliable pharmaceutical composition for treating obesity and related metabolic diseases.

The present application relates to a kind of hydrolysis targeting chimera based on artificial antibody and its preparation method and application.The hydrolysis targeting chimera is composed of artificial gold antibody and molecule that can activate cell degradation mechanism, the artificial gold antibody is composed of gold nanoparticle and polypeptide coupled on the surface of gold nanoparticle, the molecule that can activate cell degradation mechanism is coupled on the surface of gold nanoparticle.The hydrolysis targeting chimera can simultaneously bind target protein and activate cell degradation mechanism, with the performance of strong specific recognition target protein, and high stability, orientation controllable.At the same time, the hydrolysis targeting chimera can also play the molecule of activation cell degradation mechanism, such as lysosome sorting signal motif, coupled on the surface, induce clathrin-mediated endocytosis, transport the complex of target protein and chimera to lysosome and degrade.The hydrolysis targeting chimera can play multi-mode cancer treatment effect by synergistic inhibition of tumor cell proliferation, invasion pathway and apoptosis activation.

The present application relates to the field of eggshell membrane peptide, in order to solve the problem that the proportion of polypeptide with main function of anti-inflammatory effect is low in the eggshell membrane peptide product obtained by hydrolysis of existing eggshell membrane, provide an eggshell membrane peptide and a preparation method thereof, and an application of the eggshell membrane peptide in preparation of anti-inflammatory drugs, comprising: S100, after adding eggshell membrane powder into pure water, adjusting pH, then adding pepsin, enzymolysis for 2-5h, and the enzymolysis temperature is 35-38 DEG C, to obtain a first mixture; S200, the first mixture is subjected to enzyme inactivation process and ultrafiltration process to obtain a first hydrolysate and a first ultrafiltrate; S300, after adjusting the pH value of the first ultrafiltrate to 8-9, adding alkaline protease, enzymolysis for 6-10h, and the enzymolysis temperature is 36-38 DEG C, to obtain a second mixture; S400, the second mixture is subjected to ultrafiltration process to obtain a second ultrafiltrate and a second hydrolysate; S500, after drying the second hydrolysate, a first polypeptide composition is obtained. The present application explores the effect of different hydrolysate components on chondrocyte anti-inflammatory, and screens out eggshell membrane peptides with better effect.

The application discloses an anti-inflammatory soothing repair polypeptide composition containing tripeptide-125 and a preparation method and application thereof, and relates to the technical field of cosmetic polypeptides. The efficacy components of the composition comprise palmitoyl tripeptide-8, acetyl dipeptide-1 cetylester and tripeptide-125. The anti-inflammatory soothing repair polypeptide composition is prepared by using the efficacy components, a solvent, a solubilizer and a bacteriostatic agent, and has good anti-inflammatory, soothing and skin barrier repair performance.

The present invention relates to the field of polypeptide compositions, and specifically relates to a polypeptide metal chelate and the use thereof in the field of cosmetics. The polypeptide metal chelate is formed by means of the chelation of a polypeptide compound with a metal ion compound. A structure of the polypeptide compound is: [γ-Glu]n-AA, wherein n≥1, and AA is selected from glycine, alanine, valine, leucine, isoleucine, methionine (Met), proline, tryptophan, serine, tyrosine, cysteine, phenylalanine, asparagine, glutamine, threonine, aspartic acid, glutamic acid, lysine, arginine or histidine. The polypeptide zinc chelate prepared by the present invention has significant oil-controlling and anti-inflammatory effects, and can be compounded with other raw materials to prepare cosmetics with oil control and acne removal effects.

The present application relates to the technical field of biotechnology and cosmetic material technology, and particularly discloses a polypeptide composition-hydrogel composite delivery system for regulating skin sebum membrane stability, and a preparation method and application thereof. The composite delivery system comprises a polypeptide composition, a hydrogel matrix, a penetration enhancer and deionized water; and the polypeptide composition is composed of an antibacterial polypeptide, a prebiotic polypeptide and an immunomodulatory polypeptide. The preparation method comprises the following steps: dissolving the antibacterial polypeptide, the prebiotic polypeptide and the immunomodulatory polypeptide in a phosphate buffer to obtain a polypeptide composition solution; dispersing the hydrogel matrix in deionized water, mixing the penetration enhancer after stirring and swelling, adding the polypeptide composition solution dropwise, adjusting the pH value, vacuum degassing, and then freeze-drying to obtain the composite delivery system. The preparation method is simple, the obtained composite system is weakly acidic, highly matches the physiological pH value of a healthy sebum membrane, helps to maintain a weakly acidic environment on the surface of the skin for a long time, and further inhibits the reproduction of harmful bacteria from a physical environment.

The present application provides a polypeptide composition with anti-obesity effect, which is composed of capsaicin and polypeptides, and the amino acid sequence of the polypeptides comprises any one or more of SEQ ID NO. 1 to SEQ ID NO. 8. The polypeptides of sequences 1-8 are mixed uniformly at a mass ratio of 1-20:1-4:1-2:1-4:1:1:1:1, and the capsaicin is mixed with the mixed polypeptides at a ratio of 2-12:100 (w / w). The polypeptide composition proposed in the present application, in combination with capsaicin, not only promotes the weight loss effect of capsaicin, but also significantly reduces the fat index of the test mice after use, and can significantly reduce the total cholesterol concentration in the liver and the liver triglyceride concentration under a high-fat diet.

This invention discloses a *Lactococcus faecium* strain, YS-IM01. This strain can efficiently hydrolyze sheep colostrum proteins and specifically release a large amount of leucine. This invention also provides a polypeptide composition comprising multiple active peptides with potential for muscle synthesis, immunomodulation, and antioxidant activity. This invention further provides a fermented sheep colostrum product obtained by fermenting sheep colostrum with the aforementioned *Lactococcus faecium*. This invention also provides a method for preparing the fermented sheep colostrum product, comprising steps including fermentation, low-temperature vacuum concentration, and vacuum freeze-drying. Simultaneously, this invention also provides a method for preparing a drug using *Lactococcus faecium* and the polypeptide composition to prevent and / or improve age-related muscle loss.

The invention provides a compound fermentation polypeptide composition with whitening, anti-aging, moisturizing and repairing functions and application thereof, and belongs to the technical field of skin care products. The composite fermentation polypeptide composition comprises the following components: a micrococcus lysate, crithmum maritimum callus culture filtrate, a bifidobacterium fermentation product filtrate, a galactose yeast-like bacterium fermentation product filtrate, a tuber melanosporum extract, a peony root extract, acetyl hexapeptide-8, nonapeptide-1, hexapeptide-9, tetrahydromethylpyrimidine carboxylic acid, fibronectin and the like. The composite fermented polypeptide composition not only realizes multi-dimensional mildness and aging resistance, but also has the characteristics of brightening skin, enhancing skin barrier and the like, and is suitable for the field of skin care products.

The invention discloses an extracellular matrix polypeptide as well as a preparation method and application thereof. The invention provides an extracellular matrix polypeptide composition, the weight-average molecular weight of a polypeptide component in the extracellular matrix polypeptide composition does not exceed 2500Da, and the molecular weight distribution index of the polypeptide component is greater than 1.5. The extracellular matrix polypeptide provided by the invention gives consideration to reasonable control of molecular weight distribution and retention of functional peptide fragment components, has the effects of promoting cell proliferation, promoting cell antioxidation and moisturizing, relieving and the like, and is wide in application.

The present invention relates to the use of cellobiofructose (CBF) as a dietary fiber and / or prebiotic component in food products, food product additives, nutraceuticals and / or beverages or for increasing the growth of probiotic microorganisms. The invention further relates to CBF and compositions containing it for use in the prevention and / or treatment of obesity, constipation, high cholesterol, diabetes, hypertension, and / or heart disease. The invention further encompasses a method for the (recombinant) production of cellobiofructose (CBF), as described herein, using a polypeptide having levansucrase activity which comprises or consists of a polypeptide having at least 80 % sequence identity to the amino acid sequence set forth in SEQ ID NO: 1.

The invention relates to the technical field of cosmetics, in particular to a composition with a hair breakage prevention function and a preparation method and application thereof. The composition consists of plant extracts and composite polypeptide, wherein the plant extracts comprise a pseudo-ginseng root extract, a cnidium monnieri fruit extract and a coralline algae extract; the composite polypeptide comprises tripeptide-2, acetyl tetrapeptide-3 and pentapeptide-3. The composition disclosed by the invention is applied to shampoo or hair conditioners, so that the tensile strength of hair can be improved, and hair breakage can be prevented.

The application discloses an ELISA antibody detection kit for identifying Brucella gene deletion live vaccine (M5-90△26 strain) and wild strains and application. The kit comprises enzyme-linked reaction plate 1 and enzyme-linked reaction plate 2. The enzyme-linked reaction plate 1 is coated with LPS extracted from the Brucella gene deletion live vaccine (M5-90△26 strain), and the enzyme-linked reaction plate 2 is coated with a Brucella BP26 antigen epitope polypeptide combination composed of polypeptides with amino acid sequences shown in SEQ ID NO:1-2. The application is suitable for goats and sheep inoculated with the Brucella gene deletion live vaccine (M5-90△26 strain), can effectively distinguish the antibody produced by the Brucella gene deletion live vaccine (M5-90△26 strain) and the antibody produced by wild strain infection, thereby realizing identification and detection, being favorable for prevention and control of brucellosis and optimization of a vaccination strategy, and providing technical support for healthy and sustainable development of animal husbandry.

This invention proposes a method for screening bisphenol A-specific peptides using molecular simulation, belonging to the field of food safety technology. The method includes the following steps: 1) Through protein stacking and molecular docking, the molecular structure and interactions of bisphenol A-protein cocrystal compounds in a protein structure database are comprehensively analyzed to obtain the receptor protein and the original parent chain; 2) Based on the original parent chain obtained in step 1), the parent peptide is obtained by truncation of the parent peptide segment by analyzing the main active amino acids in the molecular docking results; 3) By performing virtual amino acid mutations on the parent peptide obtained in step 2), a peptide library specifically binding to bisphenol A is constructed, and molecular dynamics is used for preliminary screening of the peptides; 4) The peptide chains screened in step 3) are used as specific recognition elements, and peptides labeled with fluorescein isothiocyanate are used to form specific recognition probes. The specific recognition probes are mixed with bisphenol A, and the peptide sequences with the strongest specific binding ability to bisphenol A are screened based on the different fluorescence differences. This invention is mainly applied to the screening of specific recognition elements for toxic and harmful small molecules, active ingredients, and functional factors. This method avoids the complex preparation steps of antibodies and the cumbersome screening process of aptamers, achieving high-throughput design of specific recognition elements.

The invention provides a composite plant polypeptide composition for reducing blood glucose, repairing cells, resisting oxidation and enhancing immunity as well as a preparation method and application thereof, and belongs to the technical field of biology. The invention provides a composition. Comprising a protein peptide composed of millet protein peptide, soybean protein isolate peptide, whey protein peptide and sea buckthorn protein peptide, dietary fiber composed of chia seed powder and konjaku flour, a probiotic compound composed of bifidobacterium lactis BB-12 and lactobacillus rhamnosus LGG, fructo-oligosaccharide, balsam pear peptide, a cyclocarya paliurus extract, a corn stigma extract, a mulberry leaf extract, a vitamin E extract, a vitamin E extract, a vitamin E extract, a vitamin E extract, a vitamin E extract and a vitamin E extract. The extract mixture is composed of a radix puerariae extract, a rhizoma polygonati polysaccharide extract, a moringa oleifera leaf extract and leech powder, and the vitamin mineral substance mixture is composed of other functional components. The composition provided by the invention is matched with various proteins, dietary fibers, probiotics, prebiotics, plant extracts, vitamins, minerals, other functional components and the like, so that the purpose of effectively and synergistically improving the conditions of diabetes and nephropathy from multiple targets and multiple ways is finally achieved.

The invention relates to the technical field of cosmetics, in particular to an oil-soluble polypeptide composition of a pure oil system as well as a preparation method and application of the oil-soluble polypeptide composition. The composition comprises the following components: palmitoyl tetrapeptide-7, palmitoyl tripeptide-1, dipeptide diaminobutyryl benzyl amide diacetate, acetyl hexapeptide-8, arginine / lysine polypeptide and grease. Through compounding of palmitoyl tetrapeptide-7, palmitoyl tripeptide-1, dipeptide diaminobutyrylbenzylamide diacetate, acetyl hexapeptide-8 and arginine / lysine polypeptide, the eye cream can balance the structure and function of the skin and special problems of eyes, and has the effects of resisting oxidation and relieving inflammation; the technical problems that an existing eye care product is single in effect, high in irritation and poor in stability are solved, and the purpose of comprehensively improving the periorbital skin is achieved.

The present disclosure provides, among other things, methods and compositions for the oral administration of polypeptides. Many polypeptides are typically administered in liquid solutions by intravenous or subcutaneous injection. The present disclosure provides methods and compositions that include a polypeptide formulation, e.g., truffle, tablet, globule, candy, capsule formulation suitable for oral administration, where the formulation includes an amorphous polypeptide composition or a crystallized polypeptide composition and a pharmaceutically acceptable carrier.

The application discloses a caviar polypeptide composition with enhanced immune function, a preparation method, application and preparation, belongs to the technical field of bioactive polypeptides, and comprises at least one polypeptide with an amino acid sequence of RWFPGKPLFWRA, FWKPLRGALFPW or KWRFPGALPWFA and a molecular weight of 1400-1550 Da. The preparation method comprises caviar protein extraction, complex enzymolysis, ultrafiltration separation, chromatography purification and solid-phase synthesis. Animal experiments prove that the polypeptide can significantly enhance cell immunity, humoral immunity, monocyte-macrophage function and NK cell activity, the mechanism is related to TLR2 / TLR4 receptor activation and NF-κB / MAPK signal pathways, the safety is good, and the polypeptide is suitable for development into an immune-enhancing health food or medicine.

The invention discloses cattle spleen peptide for enhancing immunity and a preparation method, and relates to the technical field of protein peptide extraction. The cattle spleen is treated by utilizing the synergistic effect of the compound enzyme to obtain the corresponding polypeptide composition, the immune response of a body can be remarkably enhanced, the effect of enhancing the immunity is achieved, and the polypeptide composition can serve as a functional factor to be applied to a functional product for enhancing the immunity. The preparation method has the advantages of low equipment requirement, simple process, easiness in operation, high extraction efficiency and the like, and is suitable for industrial production.

The application discloses an albumin active polypeptide composition for promoting skin mucosa wound healing, which comprises the following components: albumin active polypeptide, yeast-beta-glucan, concentrated wolfberry juice, preservative, thickening agent, sweetening agent and water. The albumin active polypeptide is encapsulated by a carrier formed by cross-linking of phosphorylated microcrystalline cellulose and glycidyltrimethylammonium chloride modified chitosan, which can well protect the albumin active polypeptide, prevent the degradation of the active polypeptide by complex gastrointestinal digestive enzymes, effectively improve the retention rate of the active polypeptide in the gastrointestinal environment, significantly increase the enrichment amount of the active polypeptide in the small intestinal epithelial cells, promote the absorption of the active polypeptide by the small intestinal epithelial cells, and slowly release in the body, and then be transported to the target through blood circulation, so that the bioavailability of the active polypeptide is improved, and the ability of the composition for promoting skin mucosa wound healing is improved.

The invention discloses a polypeptide composition with functions of whitening and brightening skin, resisting inflammation, resisting light aging and repairing skin, and relates to the technical field of skin care products. The polypeptide composition is prepared from oligopeptide-1, dipeptide-15, hexapeptide-9, palmitoyl tripeptide-5, tripeptide-2, palmitoyl tetrapeptide-7 and carnosine. According to the composition, through the synergistic effect of multiple active peptides, the effects of whitening and brightening skin, resisting inflammation, resisting light aging and repairing skin are achieved through multiple ways and multiple aspects, the effects of improving wrinkles, repairing skin, brightening skin color and delaying senescence can be better achieved, and the composition can be widely applied to various types of cosmetics.

The invention relates to the technical field of skin care products, and particularly discloses an anti-aging polypeptide composition as well as a preparation method and application thereof. According to the anti-aging polypeptide composition disclosed by the invention, through a multi-target-point and multi-layer synergistic effect, efficient wrinkle smoothing, tightening and anti-aging are realized. The anti-aging polypeptide composition can act on a neuromuscular joint and a dermal matrix at the same time, and has double effects of immediate improvement and long-term repair. According to the anti-aging polypeptide composition, the active ingredients and the penetration enhancer are synergistic, and compared with exogenous supplement collagen, the anti-aging polypeptide composition can be effectively absorbed in a transdermal mode, enhance the regeneration capacity of autologous collagen, better regulate extracellular matrixes and improve the anti-aging effect of the composition, and the anti-aging polypeptide composition has the advantages that by endogenous stimulation of regeneration of structural protein such as skin collagen, the anti-aging effect of the anti-aging polypeptide composition is improved, and the anti-aging effect of the anti-aging polypeptide composition is improved. The problem of skin laxity and aging caused by loss of structural proteins such as skin collagen is thoroughly solved, and the efficient wrinkle smoothing and anti-aging effects are achieved.

The invention is suitable for the technical field of cosmetic preparations, and provides a composite polypeptide composition and a preparation method thereof. The composition is prepared from the following raw materials in parts by weight: 20 to 40 parts of mussel mucin derived peptide, 10 to 20 parts of carnosine, 20 to 35 parts of polylysine-oligopeptide 1-lactitol-sodium citrate assembly, 30 to 50 parts of fermented centella asiatica extract, 10 to 30 parts of leontopodium alpinum stem cell extract, 100 to 200 parts of phospholipid, 30 to 60 parts of cholesterol and 100 to 150 parts of emollient. The preparation method comprises the following steps: dissolving fat-soluble and partial active components by using an organic phase, dispersing or dissolving water-soluble active components and an assembly by using a water phase, and carrying out rotary evaporation film formation, hydration and high-pressure homogenization to prepare the nano co-assembled lipid carrier. Through component synergy and a nano-carrier technology, the entrapment efficiency and stability of active components are remarkably improved, the skin permeability is enhanced, the synergistic effects of moisturizing, skin barrier repairing and aging resisting are achieved, and the composition is suitable for preparing various cosmetics.

A method for designing a protein capable of binding in an RNA base selective manner or RNA base sequence specific manner is provided. The protein of the present invention is a protein containing one or more of PPR motifs (preferably 2 to 14 PPR motifs) each consisting of a polypeptide of 30- to 38-amino acid length represented by the formula 1 (wherein Helix A is a moiety of 12-amino acid length capable of forming an α-helix structure, and is represented by the formula 2, wherein, in the formula 2, A1 to A12 independently represent an amino acid; X does not exist, or is a moiety of 1- to 9-amino acid length; Helix B is a moiety of 11- to 13-amino acid length capable of forming an α-helix structure; and L is a moiety of 2- to 7-amino acid length represented by the formula 3, wherein, in the formula 3, the amino acids are numbered “i” (−1), “ii” (−2), and so on from the C-terminus side, provided that Liii to Lvii may not exist), and combination of three amino acids A1, A4 and Lii, or combination of two amino acids A4, and Lii is a combination corresponding to a target RNA base or base sequence.

The present application belongs to the technical field of biological pharmacy, and particularly relates to a polypeptide composition of a novel coronavirus, a vaccine prepared therefrom and application thereof. The polypeptide composition of the novel coronavirus comprises four polypeptides of GY, VV, VS and DL. Experiments show that the polypeptide or the composition thereof can effectively induce T cell immune response and specific humoral immunity against SARS-CoV-2 and its mutant strains, stimulate the body to produce antigen-specific antibodies, provide a basis for constructing SARS-CoV2 preventive and / or therapeutic antibodies with high affinity, high quality and strong specificity, and have high application value for prevention and treatment of the novel coronavirus.

The invention belongs to the technical field of biological medicines, and discloses an anti-tumor polypeptide composition as well as a preparation method and application thereof. The composition is prepared from the following components in parts by weight: 10 to 30 parts of mucopolysaccharide combined hybrid peptide (polypeptide A) extracted from the body wall of the sea cucumber, 5 to 15 parts of angiogenesis inhibiting peptide RGD-4C, 5 to 10 parts of tumor penetrating peptide iRGD, 3 to 8 parts of thymosin alpha 1, 2 to 6 parts of ganoderma lucidum polysaccharide and 1 to 4 parts of vitamin C palmitate. Wherein the polypeptide A is prepared by a process comprising specific ultrasonication, ultrafiltration, ion exchange chromatography and reversed-phase liquid chromatography purification. The invention also provides a preparation method of the composition, which comprises the steps of weighing, sterile mixing, grinding and sub-packaging, and can be further prepared into dosage forms such as freeze-dried powder injection and the like. The composition can be used for preparing medicines for treating solid tumors such as lung cancer, breast cancer, colorectal cancer, liver cancer and the like through synergism of multiple components and multiple target points, and can be combined with chemotherapeutic medicines such as paclitaxel and the like.

The application belongs to the technical field of food, and particularly relates to a blood protein oligopeptide solid beverage and a preparation method and application thereof. The blood protein oligopeptide solid beverage is mainly prepared from the following components by weight: 30-50 parts of blood protein oligopeptide; 15-25 parts of resistant dextrin and ferrous lactate complex; 20-30 parts of pH and enzyme double-response microsphere carrier; 8-12 parts of sea buckthorn fruit powder and vitamin C liposome; 15-25 parts of collagen peptide; and 0.5-1 part of silicon dioxide. The pH and enzyme double-response microsphere carrier is mainly composed of chitosan, pectin and trypsin cut polypeptide. The collagen peptide, blood protein oligopeptide and pH and enzyme double-response microsphere carrier are used to prepare microspheres. The blood protein oligopeptide is prepared by a fermentation method, and the pH and enzyme double-response microsphere carrier method is combined, so that the iron absorption rate and the stability of active ingredients are significantly improved.

The application discloses a collagen peptide, a collagen peptide salt, a polypeptide composition and application thereof, and belongs to the technical field of active peptide materials. The collagen peptide is a short peptide with an amino acid sequence as shown in SEQ ID NO:1 or a cyclic peptide containing the amino acid sequence as shown in SEQ ID NO:1. The collagen peptide can significantly inhibit tyrosinase activity and significantly inhibit melanin content in cells, thereby achieving a whitening effect.

A method for designing a protein capable of binding in an RNA base selective manner or RNA base sequence specific manner is provided. The protein of the present invention is a protein containing one or more of PPR motifs (preferably 2 to 14 PPR motifs) each consisting of a polypeptide of 30- to 38-amino acid length represented by the formula 1 (wherein Helix A is a moiety of 12-amino acid length capable of forming an α-helix structure, and is represented by the formula 2, wherein, in the formula 2, A1 to A12 independently represent an amino acid; X does not exist, or is a moiety of 1- to 9-amino acid length; Helix B is a moiety of 11- to 13-amino acid length capable of forming an α-helix structure; and L is a moiety of 2- to 7-amino acid length represented by the formula 3, wherein, in the formula 3, the amino acids are numbered “i” (−1), “ii” (−2), and so on from the C-terminus side, provided that Liii to Lvii may not exist), and combination of three amino acids A1, A4 and Lii, or combination of two amino acids A4, and Lii is a combination corresponding to a target RNA base or base sequence.