205 results about "Polypeptide composition" patented technology

Disclosed herein are molecules and pharmaceutical compositions that induce an insertion, deletion, duplication, or alteration in an incorrectly spliced mRNA transcript to induce exon skipping or exon inclusion. Also described herein include methods for treating a disease or disorder that comprises a molecule or a pharmaceutical composition that induces an insertion, deletion, duplication, or alteration in an incorrectly spliced mRNA transcript to induce exon skipping or exon inclusion.

The invention provides a preparation method and application of a camellia polypeptide composition with anti-wrinkle, repairing and brightening effects. The camellia polypeptide composition comprises a component A and a component B, wherein the component A comprises a wrapping solubilizer and polyhydric alcohol; component B: a plurality of polypeptides; the component C comprises carnosine, camellia polypeptide and water. The camellia polypeptide disclosed by the invention is prepared by the following steps: performing supercritical deoiling on camellia seeds, performing alkali extraction, acid precipitation and enzymolysis to prepare polypeptide, performing membrane filtration, taking micromolecule polypeptide as a main component, promoting synthesis of type I, type III and type V collagen, promoting cell migration and scavenging free radicals, and has the effects of resisting wrinkles, repairing and resisting oxidation; according to the invention, the problem of water solubility of palmitoyl peptide and acetyl peptide is solved, so that palmitoyl peptide and acetyl peptide can form a stable composition together with water-soluble polypeptide. The anti-wrinkle, repairing and whitening effects are achieved in multiple ways and multiple directions, the effects of improving wrinkles, repairing the skin, brightening the skin color and delaying senescence can be better achieved, and the anti-wrinkle, repairing and whitening composition can be widely applied to various types of cosmetics.

The invention discloses a screening method of hericium erinaceus functional polypeptide based on artificial intelligence assistance and polypeptide or a polypeptide composition. The method comprises the following steps: (1) constructing an AI auxiliary annotation model; (2) extracting hericium erinaceus crude protein; (3) preparing hericium erinaceus protein enzymatic hydrolysate through composite enzymatic hydrolysis; (4) performing polypeptide identification on the hericium erinaceus protein enzymatic hydrolysate to obtain a polypeptide sequence; and (5) carrying out AI auxiliary annotation and polypeptide screening by using a large language model based on a DeepSeek platform so as to obtain a polypeptide sequence with assumed biological activity. The polypeptide sequence and the polypeptide composition which have remarkable ACE inhibition and oxidation resistance dual functions are successfully obtained by utilizing the method disclosed by the invention. Compared with a traditional experience screening path, the method realizes a high-throughput active peptide development process which is targeted in structure, accurate in prediction and capable of verifying a closed loop.

Disclosed herein are molecules and pharmaceutical compositions that induce an insertion, deletion, duplication, or alteration in an incorrectly spliced mRNA transcript to induce exon skipping or exon inclusion. Also described herein include methods for treating a disease or disorder that comprises a molecule or a pharmaceutical composition that induces an insertion, deletion, duplication, or alteration in an incorrectly spliced mRNA transcript to induce exon skipping or exon inclusion.

The invention belongs to the technical field of biological medicine, and relates to a composite polypeptide composition as well as a preparation method and application thereof. According to the technical scheme, pH responsive polymer loaded oligopeptide-1, acetyl heptapeptide-4 and palmitoyl tripeptide-1 are adopted as a core, hyaluronic acid is adopted for wrapping, a hyaluronic acid layer adsorbs palmitoyl tripeptide-8, heptapeptide-6 and decapeptide-4, and the composite polypeptide with a core-shell structure is prepared and used for treating inflammatory skin diseases. In the acute stage of inflammation, the active ingredients on the outer layer quickly inhibit the activation of proinflammatory factors and immune cells and relieve red, swollen and heat pain, and the gel outer layer formed by hyaluronic acid realizes continuous release, prolongs the action time and supplements moisture to the skin; the pH-responsive polymer of the inner layer releases active ingredients at the inflammation part, so that relapse is prevented, and the skin barrier function is repaired. The composition plays a role from multiple dimensions of inflammation regulation, barrier repair, oxidation resistance and the like through a multi-layer structure design and a multi-component synergistic effect.

The invention provides a polypeptide composition and application thereof in treating male erectile dysfunction. Specifically, the polypeptide composition contains the erectile peptide screened and identified by the invention, and the erectile peptide has good oxidation fatigue resistance, can effectively promote penile erection, improve testosterone level and effectively treat erectile dysfunction, and has wide application prospects.

The invention discloses application of a broad-spectrum influenza virus polypeptide vaccine in preparation of a medicine for preventing influenza virus infection and controlling influenza virus reinfection, and belongs to the technical field of biological medicine. The vaccine is designed on the basis of artificially synthesized polypeptide, a polypeptide sequence which is highly conservative and has immunocompetence is screened and optimized aiming at various subtype influenza virus proteins, a broad-spectrum polypeptide vaccine P125-H is constructed, and an Ii-Key technology and an MF59 adjuvant are applied to enhance immune response. The vaccine can effectively induce immune protection against H1N1 and H7N9 influenza A viruses, significantly reduce the virus load, reduce pathological injury to the lung and improve the survival rate. The P125-H vaccine is composed of three optimized polypeptides, has a cross protection effect on various influenza virus subtypes, has the potential of being developed into a broad-spectrum influenza vaccine, and provides a new technical scheme for coping with influenza virus variation and improving vaccine efficacy.

The invention discloses a composition containing probiotics and fusion polypeptide and application of the composition in treatment of intestinal inflammatory diseases. The composition is prepared from lactobacillus plantarum (Lactobacillus plantarum CCFM8661) and a functional fusion polypeptide, wherein the amino acid sequence of the fusion polypeptide is as shown in SEQ ID NO. 1. The invention also discloses a preparation method of the composition. The fusion polypeptide can be used for directly inhibiting the growth of pathogenic bacteria and neutralizing toxins by enhancing the intestinal colonization capability of probiotics, so that the triple synergistic effect of bacteriostasis, repair and immunoregulation is realized. Experiments show that the composition significantly reduces the disease activity index in a DSS-induced mouse colitis model, recovers the colon length, and inhibits serum inflammatory factors IL-6 and TNF-alpha. In addition, the survival rate of the freeze-dried microcapsule preparation in simulated gastric juice is gt; the colon targeted release characteristic is realized. The invention provides an efficient and stable new treatment strategy for intestinal inflammatory diseases.

The present application provides a vaccine comprising: 1) a peptide oligomer consisting of 2-8 copies of a bacteriophage T4 fibrin C-terminal polypeptide and one or more antigen proteins; or 2) a coding nucleic acid of item 1) wherein 2-8 copies of the bacteriophage T4 fibrin C-terminal polypeptides in the peptide oligomer are directly linked in series through chemical bonds or each of the bacteriophage T4 fibrin C-terminal polypeptides is linked to each other through a peptide linker, and the peptide oligomer and the antigen protein are directly connected through a chemical bond or connected through a peptide linker. In the bi-T4-virus antigen vaccine platform provided by the invention, the antigen can be subjected to multi-component change, that is, the antigen can form a trimer, so that the use flexibility of the vaccine platform is enhanced, the application range of the vaccine platform is widened, and the bi-T4 structure can enhance the formation of trimer protein and enhance the immune aging.

The invention discloses a composite plant polypeptide composition with the effects of repairing pancreas and reducing blood sugar. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10 to 50 parts of ginsenoside, 5 to 30 parts of radix astragali seu hedysari extract, 3 to 20 parts of radix rehmanniae polysaccharide, 2 to 15 parts of radix trichosanthis alkaloid, 5 to 25 parts of rhizoma dioscoreae polysaccharide, 5 to 30 parts of bitter gourd polypeptide, 3 to 20 parts of folium mori alkaloid, 8 to 40 parts of rhizoma polygonati polysaccharide, 5 to 25 parts of fructus hippophae flavone, 10 to 35 parts of wheat germ peptide, 2 to 15 parts of coptis berberine, 10 to 45 parts of corn stigma polysaccharide and 3 to 20 parts of saussurea involucrata polyphenol. 1-10 parts of sophora flavescens total alkaloids, 5-25 parts of a dendrobium extract, 5-30 parts of a radix puerariae extract, 8-35 parts of L-arabinose and 10-40 parts of small molecule peptides; 20 to 100 parts of collagen peptide and 15 to 80 parts of albumin peptide; 5-50 parts of microcrystalline cellulose, 3-30 parts of fructo-oligosaccharide and 0.5-5 parts of magnesium stearate; the limitation of single sugar control or simple component mixing in the prior art is broken through, a synergistic system of plant active components and functional polypeptides is constructed, and the purity and retention rate of the plant active components are improved by optimizing the extraction process.

The invention relates to the technical field of molecular probes, and particularly discloses a molecular probe of a targeted tyrosine kinase receptor as well as a preparation method and application of the molecular probe, the molecular probe comprises radioisotope, targeted polypeptide and a chelating group, the targeted polypeptide specifically targets the tyrosine kinase receptor, the chelating group is connected with the targeted polypeptide to form a targeted precursor, and the chelating group is connected with the targeted polypeptide to form the targeted precursor. The radioactive isotope labeled precursor forms a molecular probe, and the sequence of the targeted polypeptide is shown as any one of SEQ ID No.1-8. The targeting polypeptide has good targeting property on a tyrosine kinase receptor coded by an RET gene, can realize specific high uptake in a cell line of NEPC, has relatively good biological safety, can realize accurate imaging aiming at NEPC, and clearly and durably visualizes NEPC tumors in PET imaging; meanwhile, the molecular probe can effectively delay the growth capacity of NEPC, can provide a new treatment choice for NEPC patients, and has good clinical transformation potential.

Provided herein are polypeptide compositions and chimeric antigen receptors comprising natural ligands of hematopoietic cell receptors and are thereby able to specifically bind to hematopoietic cell receptors. Also provided are methods and compositions for using the polypeptide compositions and chimeric antigen receptors in the treatment of disease, such as cancer, for editing a gene in a cell, and for culturing populations of cells.

The invention discloses a preparation method of a polypeptide composition with anti-wrinkle and firming effects, and belongs to the technical field of cosmetic raw material preparation, and the preparation method comprises the following steps: S1, raw material pretreatment; s2, mixing the raw materials; s3, adjusting the pH value: adjusting the pH value of the polypeptide mixed solution to 5.5-6.5 by using a hydrophosphate or citric acid solution; s4, filtering and degerming: filtering and degerming the polypeptide mixed solution of which the pH value is adjusted through a 0.22 mu m microporous filter membrane to obtain a sterile polypeptide composition solution; and S5, filling: filling the sterile polypeptide composition solution into a proper packaging container. Through synergistic compatibility of five polypeptides such as palmitoyl pentapeptide-3 and acetyl hexapeptide-8, the anti-wrinkle and firming effects are achieved from four mechanisms of stimulating collagen regeneration, inhibiting neural signals, blocking protease degradation and resisting oxidation.

The application discloses a polypeptide composition for treating obesity and related metabolic diseases and application thereof. The polypeptide composition comprises polypeptide 1 and polypeptide 2, wherein the amino acid sequence of the polypeptide 1 is shown as SEQ ID NO. 1, can be specifically combined with the CD105 receptor on the surface of MSCs and the AdipoR1 receptor of adipocytes; the amino acid sequence of the polypeptide 2 is shown as SEQ ID NO. 2, can target adipose tissue and regulate glycolipid metabolism. The polypeptide composition is combined with fat-derived mesenchymal stem cells (MSCs) and applied to a high-fat-diet obese mouse model, and the results show that the composition has a significant synergistic effect in reducing body fat, improving the morphology of adipose tissue, regulating serum metabolic factors and inflammatory factors, and has no obvious organ toxicity. The application provides a new effective strategy and a safe and reliable pharmaceutical composition for treating obesity and related metabolic diseases.

The invention discloses an antioxidant and firming polypeptide composition and a preparation method thereof, and the antioxidant and firming polypeptide composition comprises the following components in percentage by weight: 50-75% of water, 5-10% of butanediol, 0.1-3% of panthenol and 0.5-1% of p-hydroxyacetophenone. 0.2 to 8% of hydroxyethyl piperazine ethanesulfonic acid; the composition is prepared from the following components in percentage by weight: 20-30% of an eggshell membrane extract, 0.01-2% of nonapeptide-1, 0.01-5% of acetyl hexapeptide-8 and 0.5-1% of 1, 2-hexanediol; the preparation method comprises the following steps: putting water, butanediol, panthenol and p-hydroxyacetophenone into a container, and stirring at a constant temperature to obtain a first mixed solution; adding hydroxyethyl piperazine ethanesulfonic acid into the first mixed solution, and uniformly stirring to obtain a second mixed solution; adding an eggshell membrane extract, nonapeptide-1, acetyl hexapeptide-8 and 1, 2-hexanediol into the second mixed solution, and uniformly stirring to obtain the antioxidant and firming polypeptide composition; the antioxidant and firming polypeptide composition disclosed by the invention has the functions of resisting oxidation, firming and brightening the skin; the method for preparing the antioxidant and firming polypeptide composition is simple and convenient for large-scale production.

The present application relates to a kind of hydrolysis targeting chimera based on artificial antibody and its preparation method and application.The hydrolysis targeting chimera is composed of artificial gold antibody and molecule that can activate cell degradation mechanism, the artificial gold antibody is composed of gold nanoparticle and polypeptide coupled on the surface of gold nanoparticle, the molecule that can activate cell degradation mechanism is coupled on the surface of gold nanoparticle.The hydrolysis targeting chimera can simultaneously bind target protein and activate cell degradation mechanism, with the performance of strong specific recognition target protein, and high stability, orientation controllable.At the same time, the hydrolysis targeting chimera can also play the molecule of activation cell degradation mechanism, such as lysosome sorting signal motif, coupled on the surface, induce clathrin-mediated endocytosis, transport the complex of target protein and chimera to lysosome and degrade.The hydrolysis targeting chimera can play multi-mode cancer treatment effect by synergistic inhibition of tumor cell proliferation, invasion pathway and apoptosis activation.

The invention discloses a polypeptide composition for promoting skin desquamation and a preparation method and application thereof, the polypeptide composition comprises polypeptides and a polyol preservative, the polypeptides comprise a first polypeptide, a second polypeptide and a third polypeptide, and the first polypeptide is acetyl tetrapeptide-2; the second polypeptide comprises one or more of hyaluronic acid polypeptide, red scorpion toxin and palmitoyl tripeptide-5; and the third polypeptide is prepared from one or more of acetyl octapeptide-3, palmitoyl dipeptide-7, acetyl tetrapeptide-9 and acetyl tetrapeptide-11. The polypeptide composition can significantly up-regulate the gene expression of kallikrein through a synergistic effect and promote old cutin exfoliation; meanwhile, gene expression of metalloproteinase is inhibited, and light aging is delayed. The polypeptide composition disclosed by the invention can be prepared into various cosmetics and has multiple effects of promoting skin desquamation, resisting wrinkles and repairing barriers.

The invention belongs to the field of biological medicine, and particularly relates to an oral polypeptide composition for improving gastrointestinal tract absorption of polypeptide medicine. Specifically disclosed is an oral polypeptide composition comprising polypeptide molecules, medium-chain fatty acids and salts thereof, amino acids, and N-(8-(2-hydroxybenzoyl) amino) caprylic acid (NAC) and salts thereof. The oral polypeptide preparation disclosed by the invention can effectively improve the blood concentration of the polypeptide and improve the bioavailability of the oral polypeptide.

The present disclosure provides, among other things, methods and compositions for the oral administration of semaglutide polypeptides. The present disclosure provides methods and compositions that include a semaglutide polypeptide formulation, e.g., tablet formulation suitable for oral administration, where the formulation includes a crystallized polypeptide composition and a pharmaceutically acceptable carrier.

This Fc-cell complex comprises a cell and an antibody Fc region bound to a surface of the cell. In the antibody Fc region, two Fc constituent polypeptides are disulfide-bonded to each other. The antibody Fc region is chemically bonded to the cell surface via a linker introduced into a functional group in a side chain of a specific amino acid residue present at one or more positions of the two antibody Fc constituent polypeptides.

The FSH and LH fusion protein is composed of a first polypeptide, a second polypeptide, a third polypeptide and a fourth polypeptide. Wherein the first polypeptide comprises a beta subunit of FSH, a beta subunit of LH, a hinge, a CH2 structural domain and a CH3 structural domain, or the beta subunit of LH, the beta subunit of FSH, the hinge, the CH2 structural domain and the CH3 structural domain from an N terminal to a C terminal; the second polypeptide comprises a hinge, a CH2 structural domain and a CH3 structural domain from the N terminal to the C terminal; the third polypeptide and the fourth polypeptide are both composed of alpha subunits of FSH. The FSH and LH fusion protein has the functions of FSH and LH at the same time; meanwhile, compared with recombinant or natural FSH and LH, the heterodimer has a longer half-life period, can complement gonadotropin in a long-acting, mild and continuous manner, and is suitable for various FSH and LH deficiency related diseases such as male hypogonadotropin gonad hypofunction, female pituitary insufficiency, hypogonadotropin amenorrhea or hypogonadotropin infertility. The medicine economy is better, and the patient compliance is better.

The present application relates to an oral polypeptide composition system stably absorbed in the gastrointestinal tract, and specifically discloses an oral polypeptide composition. The composition comprises a polypeptide molecule (A), a surfactant (B), an amino acid monomolecular or a bimolecular combination (C). The molecular weight of a peptide drug used as a drug in the oral polypeptide composition is within a range of 0.1 kDa to 20 kDa. The action part of the composition system is small intestine or large intestine; and the polypeptide composition has obviously improved and increased stability and absorbability in the gastrointestinal tract.

The present application relates to the field of eggshell membrane peptide, in order to solve the problem that the proportion of polypeptide with main function of anti-inflammatory effect is low in the eggshell membrane peptide product obtained by hydrolysis of existing eggshell membrane, provide an eggshell membrane peptide and a preparation method thereof, and an application of the eggshell membrane peptide in preparation of anti-inflammatory drugs, comprising: S100, after adding eggshell membrane powder into pure water, adjusting pH, then adding pepsin, enzymolysis for 2-5h, and the enzymolysis temperature is 35-38 DEG C, to obtain a first mixture; S200, the first mixture is subjected to enzyme inactivation process and ultrafiltration process to obtain a first hydrolysate and a first ultrafiltrate; S300, after adjusting the pH value of the first ultrafiltrate to 8-9, adding alkaline protease, enzymolysis for 6-10h, and the enzymolysis temperature is 36-38 DEG C, to obtain a second mixture; S400, the second mixture is subjected to ultrafiltration process to obtain a second ultrafiltrate and a second hydrolysate; S500, after drying the second hydrolysate, a first polypeptide composition is obtained. The present application explores the effect of different hydrolysate components on chondrocyte anti-inflammatory, and screens out eggshell membrane peptides with better effect.

The application discloses an anti-inflammatory soothing repair polypeptide composition containing tripeptide-125 and a preparation method and application thereof, and relates to the technical field of cosmetic polypeptides. The efficacy components of the composition comprise palmitoyl tripeptide-8, acetyl dipeptide-1 cetylester and tripeptide-125. The anti-inflammatory soothing repair polypeptide composition is prepared by using the efficacy components, a solvent, a solubilizer and a bacteriostatic agent, and has good anti-inflammatory, soothing and skin barrier repair performance.

The invention provides a characteristic protein spectrum model for detecting uremia and a construction method. Wherein the marker polypeptide composition is mainly carbamoyl hemoglobin of alpha / beta globin, and comprises eight marker polypeptides with the following mass-to-charge ratios: 7564 m / z, 7585 m / z, 7934 m / z, 7956 m / z, 8476 m / z, 15127 m / z, 15170 m / z, 15868 m / z, 15911 m / z and 16952 m / z. The mass spectrum model disclosed by the invention can be used for detecting uremia. The detection method provided by the invention is simple, easy to operate and high in accuracy, and provides a new method and idea for uremia detection.

The invention discloses a composition containing polypeptide and collagen and application of the composition. The composition comprises the polypeptide and the collagen. The polypeptide is prepared from palmitoyl pentapeptide-4, decarboxylated carnosine, acetyl octapeptide-3 and hexapeptide-11; the collagen is a recombinant XVII type collagen; the mass ratio of the polypeptide to the collagen is (12: 5)-(12: 25). The recombinant XVII type collagen is added into the polypeptide composition, on one hand, the permeability of the polypeptide composition can be remarkably improved, on the other hand, the generation of the collagen can be improved, and compounding of the polypeptide composition and the recombinant XVII type collagen can achieve synergistic interaction and improve relative expression of COL7A1 and COL17A1 genes, so that the overall anti-aging effect is enhanced.

The present invention relates to the field of polypeptide compositions, and specifically relates to a polypeptide metal chelate and the use thereof in the field of cosmetics. The polypeptide metal chelate is formed by means of the chelation of a polypeptide compound with a metal ion compound. A structure of the polypeptide compound is: [γ-Glu]n-AA, wherein n≥1, and AA is selected from glycine, alanine, valine, leucine, isoleucine, methionine (Met), proline, tryptophan, serine, tyrosine, cysteine, phenylalanine, asparagine, glutamine, threonine, aspartic acid, glutamic acid, lysine, arginine or histidine. The polypeptide zinc chelate prepared by the present invention has significant oil-controlling and anti-inflammatory effects, and can be compounded with other raw materials to prepare cosmetics with oil control and acne removal effects.

The invention belongs to the technical field of biological medicines, and particularly relates to a composite active polypeptide composition with a weight losing effect and a preparation method thereof. The composite active polypeptide composition comprises NT analogue polypeptide ELP3, mulberry leaf polypeptide, balsam pear polypeptide, sea cucumber polypeptide and oyster peptide; the amino acid sequence of the NT analogue polypeptide ELP3 is as shown in SEQ ID NO. 1. According to the present invention, the artificial peptide fragment AYYKSQWLVNGEGTP is added to the end of the wild type NT, such that the gastric acid secretion can be inhibited, and the activity of the alpha-amylase can be significantly inhibited; the NT analogue polypeptide ELP3 and various polypeptide components are matched to achieve a synergistic effect, so that the blood fat metabolism condition of obese mice can be effectively improved, and the fat weight of the mice is reduced; meanwhile, the food intake of the mice can be remarkably inhibited, so that the purpose of reducing the weight of the mice is achieved, and a good weight-losing effect is achieved.

The present application relates to the technical field of biotechnology and cosmetic material technology, and particularly discloses a polypeptide composition-hydrogel composite delivery system for regulating skin sebum membrane stability, and a preparation method and application thereof. The composite delivery system comprises a polypeptide composition, a hydrogel matrix, a penetration enhancer and deionized water; and the polypeptide composition is composed of an antibacterial polypeptide, a prebiotic polypeptide and an immunomodulatory polypeptide. The preparation method comprises the following steps: dissolving the antibacterial polypeptide, the prebiotic polypeptide and the immunomodulatory polypeptide in a phosphate buffer to obtain a polypeptide composition solution; dispersing the hydrogel matrix in deionized water, mixing the penetration enhancer after stirring and swelling, adding the polypeptide composition solution dropwise, adjusting the pH value, vacuum degassing, and then freeze-drying to obtain the composite delivery system. The preparation method is simple, the obtained composite system is weakly acidic, highly matches the physiological pH value of a healthy sebum membrane, helps to maintain a weakly acidic environment on the surface of the skin for a long time, and further inhibits the reproduction of harmful bacteria from a physical environment.

The present application provides a polypeptide composition with anti-obesity effect, which is composed of capsaicin and polypeptides, and the amino acid sequence of the polypeptides comprises any one or more of SEQ ID NO. 1 to SEQ ID NO. 8. The polypeptides of sequences 1-8 are mixed uniformly at a mass ratio of 1-20:1-4:1-2:1-4:1:1:1:1, and the capsaicin is mixed with the mixed polypeptides at a ratio of 2-12:100 (w / w). The polypeptide composition proposed in the present application, in combination with capsaicin, not only promotes the weight loss effect of capsaicin, but also significantly reduces the fat index of the test mice after use, and can significantly reduce the total cholesterol concentration in the liver and the liver triglyceride concentration under a high-fat diet.