438 results about "Polypeptide composition" patented technology

Disclosed is a novel Lepidopteran- and Coleopteran-active delta-endotoxin polypeptide, and compositions comprising the polypeptide, peptide fragments thereof, and antibodies specific therefor. Also disclosed are vectors, transformed host cells, and transgenic plants that comprise nucleic acid segments encoding the polypeptide. Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising the novel sequences of the invention, as well as methods for controlling an insect population, such as the Western Corn Rootworm and Colorado potato beetle, and for conferring to a plant population resistance to the target insect species.

Disclosed are Coleopteran-toxic B. thuringiensis delta -endotoxins, nucleic acid sequences, and transgenic plants expressing these genes. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells.

The invention is based, at least in part, on the development of stabilized binding molecules that consist of or comprise a stabilized scFv and methods for making such stabilized molecules.

Disclosed are novel insecticidal polypeptides, and compositions comprising these polypeptides, peptide fragments thereof, and antibodies specific therefor. Also disclosed are vectors, transformed host cells, and transgenic plants that contain nucleic acid segments that encode the disclosed delta-endotoxin polypeptides. Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising these polynucleotide sequences, as well as methods for controlling an insect population, such as Colorado potato beetle, southern corn rootworm and western corn rootworm, and for conferring to a plant resistance to a target insect species.

The present invention provides means to improve the chemical stability of aqueous, parenteral pharmaceutical compositions comprising a polypeptide and glycerin. Reactive aldehydes are identified in commercial glycerins, and means for reducing such are provided. Convenient means are provided to assay for reactive aldehydes in glycerin, and a strong linear correlation between the level of reactive aldehydes in glycerin and chemical stability of compositions comprising a polypeptide and glycerin is demonstrated. The invention includes aqueous compositions comprising a polypeptide and glycerin having improved chemical stability compared to compositions previously known.

A CD20-binding polypeptide composition of the invention comprises at least one polypeptide selected from the group consisting of a polypeptide having the amino acid residue sequence of SEQ ID NO: 1 (Vh of 2B8 antibody), but which includes at least one amino acid residue substitution in SEQ ID NO: 1 selected from the group consisting of V₁₂K, A₁₄P, M₂₀V, I₄₈T, A₆₈T, Q₈₂E, T₈₇R, S₉₁T, and T₁₀₆W; a polypeptide having the amino acid residue sequence of SEQ ID NO: 4 (Vk of 2B8 antibody), but which includes at least one amino acid residue substitution in SEQ ID NO: 4 selected from the group consisting of L₁₁I, S₁₂T, S₂₇T, V₂₉A, G₄₀T, V₅₉S, S₆₉T, L₇₂M, R₇₆S, and V₇₇L; a polypeptide having the amino acid residue sequence of SEQ ID NO: 9 (Vh of Leu16 antibody), but which includes at least one amino acid residue substitution in SEQ ID NO: 9 selected from the group consisting of V₁₂K, M₂₀V, A₆₈T, Q₈₂E, T₈₇R, S₉₁T, D₉₃V, and A₁₁₄T; and a polypeptide having the amino acid residue sequence of SEQ ID NO: 11 (Vk of Leu16 antibody), but which includes at least one amino acid residue substitution in SEQ ID NO: 11 selected from the group consisting of L₁₁I, S₁₂T, A₅₉S, S₆₉T, L₇₂M, R₇₆S, and V₇₇L. The compositions are useful for diagnosis and treatment of autoimmune diseases.

Disclosed herein are novel peptide linkers and polypeptide compositions comprising the linkers (e.g., chimeric polypeptides) and methods of using the polypeptide compositions. The compositions and methods are particularly useful for targeting/delivering a polypeptide or protein of interest (e.g., a therapeutic polypeptide) to a cell, tissue or organ of interest in order to treat various diseases or disorders (e.g., lysosomal storage disorders).

Polynucleotide delivery-enhancing polypeptides are admixed or complexed with, or conjugated to, nucleic acids for enhancing delivery the nucleic acids into cells. The transported nucleic acids are active in target cells as small inhibitory nucleic acids (siNAs) that modulate expression of target genes, mediated at least in part by RNA interference (RNAi). The siNA/polypeptide compositions and methods of the invention provide effective tools to modulate gene expression and alter phenotype in mammalian cells, including by altering phenotype in a manner that eliminates disease symptoms or alters disease potential in targeted cells or subject individuals to which the siNA/polypeptide compositions are administered.

The present invention relates to purified peptidomimetic macrocycles. The invention additionally provides methods of preparing and using such macrocycles, for example in therapeutic applications.

The present invention relates to an in vitro diagnostic method for malaria in an individual comprising placing a tissue or a biological fluid taken from an individual in contact with a molecule or polypeptide composition, wherein said molecule or polypeptide composition comprises one or more peptide sequences bearing all or part of one or more T epitopes of the proteins resulting from the infectious activity of P. falciparum, under conditions allowing an in vitro immunological reaction to occur between said composition and the antibodies that may be present in the tissue or biological fluid, and in vitro detection of the antigen-antibody complexes formed. The invention further relates to a polypeptide comprising at least one T epitope from a liver-stage specific protein produced by P. falciparum and a vaccine composition directed against malaria comprising a molecule having one or more peptide sequences bearing all or part of one or more T epitopes resulting from the infectious activity of P. falciparum in the hepatic cells.

Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include an amino acid sequence, and variants and fragments thereof, for an antipathogenic polypeptide that was isolated from a fungal fermentation broth. Nucleic acid molecules that encode the antipathogenic polypeptides of the invention, and antipathogenic domains thereof, are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention are also disclosed.

The invention provides protein adhesives and methods of making and using such adhesives. The protein adhesives contain ground plant meal or an isolated polypeptide composition obtained from plant biomass.

The invention discloses a polypeptide composition for hair growth. The polypeptide composition comprises polypeptides capable of promoting collagen production and polypeptides capable of promoting keratoprotein production, wherein concentration of each kind of polypeptides is 0.01ppm-50,000ppm (w/w). Aiming at various kinds of alopecia or hair loss symptoms, the polypeptide composition is capable of promoting new hair growth and reinforcing roots of grown hair to avoid hair loss, so that the purpose that the hair grows longer and thicker is achieved. The polypeptide composition for hair growth can be used for cosmetics and drugs.

Disclosed are methods and compositions comprising native, site-specifically mutagenized, and synthetic peptides comprising portions of the human estrogen receptor, or estrogen receptor co-activator, and nucleic acid compositions encoding these polypeptide compositions. Also disclosed are methods for synthesizing phosphotyrosyl and malonyltyrosyl peptide derivatives and their use as antiestrogen compositions in the treatment of breast cancers, the preparation of pharmaceutical compositions, diagnostic kits, and the development of related assays for use in antitumor therapies.

The invention provides protein adhesives and methods of making and using such adhesives. One type of protein adhesive described herein contains lignin and ground plant meal or an isolated polypeptide composition obtained from plant biomass. Other types of protein adhesives described herein contain a plant protein composition and either a hydroxyaromatic/aldehyde, urea/aldehyde, or amine/aldehyde component.

The present invention relates to methods of direct pulmonary delivery of polypeptides e.g. of domain antibodies, and to particular polypeptide compositions suitable for direct pulmonary delivery. The invention also relates to use of such compositions in medicine, e.g. for the treatment and diagnosis of lung disease, for example for treating Chronic Obstructive Pulmonary Disease (COPD) and asthma.

Compositions containing at least one KAP polypeptide and at least one cosmetic ingredient chosen from conditioning agents, care agents and/or treating agents for keratin substrates, and mixtures thereof. The invention also relates to a cosmetic process for caring for, strengthening and/or repairing keratin substrates, in particular keratin fibres, using the compositions.

The invention provides protein adhesives containing certain additives and methods of making and using such adhesives. The protein adhesives contain ground plant meal or an isolated polypeptide composition obtained from plant biomass in combination with certain additives, such as an exfoliated clay or partially exfoliated clay.

Pharmaceutical compositions relating to vasoactive intestinal polypeptides and methods for the treatment of metabolic disorders, including diabetes, insulin resistance, metabolic acidosis and obesity are presented. Methods of using the vasoactive intestinal polypeptide compositions are also disclosed.

Pharmaceutical compositions relating to vasoactive intestinal polypeptides and methods for the treatment of metabolic disorders, including diabetes, insulin resistance, metabolic acidosis and obesity are presented. Methods of using the vasoactive intestinal polypeptide compositions are also disclosed.

The invention discloses a polypeptide composition for smoothing wrinkles. The composition comprises an acetylcholine agonist and a polypeptide preventing fusing of vesicae and cytomembrane to inhibit acetylcholine release. The polypeptide composition can be prepared into freeze-dried powder and solution, and can be made into various skin care products and medical products to be used for smoothing wrinkles.