18 results about "Theaflavine" patented technology

Provided is a composition comprising 20-40 parts by weight of cordyceps militaris powder, 3-25 parts by weight of theaflavins, and 1.25-4.2 parts by weight of astaxanthin, which is a whitening composition or a melanin synthesis signal antagonizing composition. The composition can be used as a main ingredient in whitening food or health food.

The invention discloses a preparation method of high-activity theaflavin. The method sequentially comprises the following steps: preparation and enzyme purification of tea powder, ultrasonic-assisted enzymolysis, oil-water double-liquid phase step type fermentation and online extraction, chromatographic column separation and purification, and membrane concentration and drying. According to the method, an oil-water double-liquid-phase enzymatic oxidation system is constructed, the fermentation process and the extraction process are coupled, the characteristic that the target product theaflavin-3, 3 '-digallate has a higher partition coefficient in an organic phase is utilized, in-situ separation and enrichment in the reaction process are achieved, and product degradation is avoided. In combination with a subsequently adopted specific chromatographic separation technology, the content of the high-activity TF3 monomer in the product is finally successfully increased to 50% or above of the total theaflavin monomer, and the biological activity of the product is improved.

The invention relates to application of theaflavin as a UGP2 inhibitor and application of theaflavin in preparation of a medicine for preventing and / or treating hepatocellular carcinoma, and belongs to the technical field of biology. According to the invention, the monomeric compound theaflavin derived from a natural product can be used as a UGP2 direct targeting inhibitor for the first time, and is used for preparing a liver cancer treatment medicine for regulating and controlling glucose metabolism reprogramming. The theaflavin can be specifically and directly combined at a UGP2 active site in a targeting manner through high activity to inhibit the activity of the UGP2, is the first specific inhibitor which takes the UGP2 as a target spot and is reported at present, and can be used for remarkably inhibiting tumor phenotypes such as proliferation, migration and invasion of liver cancer cells by inhibiting UGP2-mediated UDPG synthesis and intracellular glycogen accumulation.

The invention discloses application of a theaflavin compound in preparation of a medicine for inhibiting beta amyloid peptide aggregation, and belongs to the technical field of biological medicine. Multi-dimensional experiments prove that the galloyl group in the theaflavin compound can significantly inhibit the formation of Abeta42 amyloid fibers, reduce the generation of toxic oligomers and reconstruct formed oligomers by increasing binding sites with Abeta42 polypeptide and enhancing polar interaction and hydrophobic interaction. The TF3 (containing two galloyl groups) has the optimal anti-Abeta 42 polypeptide aggregation activity, a precise target spot is provided for design of an Abeta amyloid aggregation inhibitor taking the galloyl groups as a core structure, and an experimental basis is provided for application of a natural polyphenol compound in preparation of a medicine for preventing or treating Alzheimer's disease.

The present invention relates to an emulsifier, an emulsification composition, an oral cleansing agent, and an oral cleansing composition, each comprising at least one of theaflavin and thearubigin. The present invention also relates to an emulsification method and an oral cleansing method, each using at least one of theaflavin and thearubigin.

This invention relates to the field of black tea fermentation technology, and discloses a black tea fermentation device and method. The black tea fermentation device includes a fermentation tank, with a drive shaft rotatably connected to the center of the tank's inner cavity. Cross plates are fixedly connected to both sides of the drive shaft, and driven shafts are fixedly connected between each end of the cross plates on both sides. The device also includes multiple fermentation trays, each mounted on a driven shaft. Through gear transmission and speed increase, a piston plate is driven to slide reciprocally within a piston chamber, achieving alternating pressure reduction and pressurization for pulsating oxygen supply. During pressurization, high-pressure airflow penetrates the tea layer, forcibly providing positive pressure oxygen supply to eliminate dead zones, ensuring synchronous oxidation reactions and enhancing enzyme activity. During pressure reduction, carbon dioxide and hot, humid waste gas are rapidly discharged to prevent suffocation and heartburn. This simulates the rhythm of biological respiration, significantly promoting the formation of theaflavins and thearubigins, and optimizing biochemical reactions.

This invention relates to the field of tea processing technology and discloses a method for increasing the yield of theaflavins using alternating catalysis of exogenous enzymes. The exogenous enzyme is crude pear polyphenol oxidase solution, which is directly prepared from fruit. The method includes the following steps: first, tanninase is added to construct the conversion basis of the crude pear polyphenol oxidase solution; then, the crude pear polyphenol oxidase solution is added, allowing the enzymatic hydrolysis to be completed at room temperature with basic stirring without fermentation, thereby increasing the yield and quality of theaflavins. This invention enables the stepwise and directional conversion of ester-type catechins in tea polyphenol solutions into non-ester-type catechins, laying the foundation for subsequent conversion by exogenous enzymes. The conversion of exogenous enzymes does not require further fermentation and can be carried out at room temperature, resulting in a nearly two-fold increase in theaflavin yield.

This invention discloses an anti-muscle aging composition containing theaflavins (TF) from black tea extract and phytosterols, and its applications, belonging to the biomedical field. The composition consists of theaflavins (TF) and phytosterols (PS). Experimental verification showed that theaflavins at 1 μM and phytosterols at 1000 ppm exhibited a significant synergistic protective effect against D-galactose-induced senescence of C2C12 myoblasts. Experimental results also showed that the composition significantly enhanced cell viability and inhibited the accumulation of reactive oxygen species (ROS), with a cell viability synergistic coefficient of 0.034 and a ROS oxidative stress synergistic coefficient of 0.366, both demonstrating strong synergistic effects. The composition provided by this invention can be used to prepare drugs, functional foods, or nutritional supplements for the prevention, improvement, and / or treatment of muscle aging, featuring safety, high efficiency, and readily available raw materials, providing a new direction for the development of anti-muscle aging related products.

The application discloses a tea characteristic component composition for reducing acrylamide in Maillard reaction, and the composition is prepared by compounding at least three of five substances, i.e., epicatechin (EC), gallocatechin (GC), catechin gallate (CG), theabrine and theaflavine, and the compounded composition has the effect of reducing acrylamide, a by-product of Maillard reaction in food. In the composition of the application, the composition composed of epicatechin (EC), gallocatechin (GC), catechin gallate (CG) and theabrine has the best effect of reducing acrylamide, a by-product of Maillard reaction, and the reduction rate of acrylamide in a simulation system can be up to 75%. The composition of the application can be applied to baked food or fried food, especially baked food such as bread, cookie, potato chip and the like.

This invention discloses a sterilization method for dairy products. The invention comprises two parts: the preparation of a composite microparticle agent and synergistic magnetic induction electric field sterilization. The composite microparticles are Fe... 2+ An aspartic acid complex serves as the outer antibacterial component, while okra mucilage, theaflavins, and chickpea defensins form the core antibacterial component, constructing a composite microparticle structure with a progressive effect from antibacterial to antibacterial action. During the sterilization process, the composite microparticles are added to liquid dairy products, stirred evenly, and then a magnetic induction electric field is activated. The magnetic induction electric field, in conjunction with the composite microparticles, achieves highly efficient killing of bacteria and spores in the dairy products. This solves the problems of interaction and poor stability between antibacterial components and dairy product components, as well as the difficulty in killing spores at low temperatures. It also reduces the destruction of nutritional components in dairy products, and the Fe in the composite microparticles... 2+ Aspartic acid and theaflavins all have certain nutritional value and are suitable for use in dairy products.

The present application provides a probiotic preparation and its use. The probiotic preparation of the present application comprises active ingredients and adjuvants, wherein the active ingredients comprise oxygen scavengers, organic acid producers and place holders; the adjuvants comprise flavoring agents, oligosaccharides and other additives; wherein the oligosaccharides are not lactulose, stachyose, raffinose, oligogentiobiose and oligochitosan, and the other additives are not theaflavins, ethylenediaminetetraacetic acid disodium salt and sorbic acid. The present application effectively promotes the metabolic activity of probiotics through a specific combination of adjuvants, rather than just providing physical protection or nutritional support. Experimental data show that the adjuvant combination of the present application can significantly and continuously improve the oxidation-reduction potential and Na + K + ATPase activity of probiotics. Moreover, the probiotic preparation of the present application can effectively improve functional gastrointestinal diseases and has a bidirectional regulation function, which is suitable for different intestinal motility disorders, such as diarrhea and constipation.

The application provides a bio-based Mannich base, a preparation method thereof, an application thereof, and a TDI curing agent prepared by using the bio-based Mannich base as a catalyst. The bio-based Mannich base is obtained by a Mannich reaction of natural phenol, dimethylamine and formaldehyde, wherein the natural phenol is one or more selected from the group consisting of sesamol, catechin, theaflavin, daidzein and procyanidin. The Mannich base catalyst prepared by using the natural phenol in the application improves steric hindrance, and the reaction selectivity of small molecule monomers can be improved through the steric hindrance effect. The content of free TDI monomers in the TDI curing agent can be reduced by adding the catalyst at one time, so that the free monomers in the prepared TDI curing agent are less than 0.5% advantageously.

The invention discloses a digital twinning-based tea leaf self-adaptive fermentation cabin environment regulation and control system and method, and the system comprises a physical layer which comprises at least one tea leaf fermentation cabin; the data interaction layer is in communication connection with the physical layer; the digital twin layer comprises a high-fidelity virtual fermentation cabin model which is in one-to-one correspondence with the physical layer fermentation cabins; and the application layer provides a human-computer interaction interface. According to the invention, through the multi-source sensing array deployed in the fermentation cabin, high-frequency, in-situ and spatial distributed acquisition of multi-dimensional physical and chemical parameters and apparent images of the fermentation microenvironment is realized for the first time; the evolutionary trajectory and spatial distribution of key quality components (such as theaflavin and thearubigins) in a pile can be simulated and predicted in real time with high temporal-spatial resolution, and a traditional'black box 'process is converted into a transparent and predictable'white box' process.

Disclosed is a method of preparing black tea extract with high theaflavin content, which utilizes an enzyme solution prepared by extracting polyphenol oxidase and laccase from bergamot yam (Dioscorea opposita foshou) or Chinese yam (Dioscoreae Rhizoma). Also disclosed is a method of treating liver fibrosis or kidney injury or atherosclerosis in a subject.

The application discloses a theaflavins photothermal targeted nano-enzyme as well as a preparation method and application thereof. Theaflavins are used as a reducing agent and a capping agent, and the nano-enzyme of cerium oxide can be synthesized without high temperature and high pressure, and is coordinated with iron ions to construct a metal polyphenol network to coat the nano-enzyme of cerium oxide, and the nano-enzyme is further modified by trehalose which has affinity to bacteria, so that a novel photothermal targeted nano-enzyme is developed. The theaflavins photothermal targeted nano-enzyme has good antioxidant, peroxidase-like, photothermal and antibacterial effects, and can be used for the treatment of bacterial infection.

The invention discloses a method for efficient enzymatic synthesis of theaflavin by using ternary composite polyphenol oxidase, and belongs to the technical field of biosynthesis. The process of the method comprises the following steps: taking mushrooms and bananas as enzyme sources of polyphenol oxidase, and extracting with a buffer solution to obtain a crude enzyme solution; the preparation method comprises the following steps: dissolving and extracting instant green tea powder and hot water, extracting catechin by using ethyl acetate, and carrying out rotary evaporation and vacuum drying to obtain a mixed catechin substrate; dissolving the crude enzyme liquid and a mixed catechin substrate in a buffer solution, adding commercial polyphenol oxidase, introducing oxygen to react for a period of time at a proper temperature, extracting a synthetic product with ethyl acetate, and carrying out rotary evaporation and freeze drying to obtain a theaflavin product. The conversion rate of the obtained theaflavin is 40-55% in terms of total catechin substrates, and the method is simple in process step, low in equipment investment, free of enzyme purification, low in cost and capable of achieving efficient theaflavin conversion.

The invention discloses a theaflavin-loaded chitosan / beta-lactoglobulin nano composite material and a preparation method thereof, and belongs to the technical field of tea and food processing. The method comprises the following steps: sequentially carrying out first mixing on theaflavin and a chitosan hydrochloride solution, carrying out second mixing on theaflavin and a carboxymethyl chitosan solution, carrying out third mixing on theaflavin and a beta-lactoglobulin solution, and carrying out centrifugal treatment on a mixed solution obtained by the third mixing to obtain the theaflavin-chitosan / beta-lactoglobulin nano composite material, the pH (Potential of Hydrogen) value of the carboxymethyl chitosan solution is 5.8 to 6.2. According to the method disclosed by the invention, the bio-accessibility and stability of theaflavin can be improved.

The invention discloses a plant growth regulator for prolonging the flowering phase of garden flowers and a preparation method and application thereof, and belongs to the technical field of plant growth regulators. The plant growth regulator comprises theaflavin, huperzine B, elaeagnus pungens leaf oil, salicylic acid, calcium chloride and 6-benzylaminopurine. The theaflavin, the huperzine B and the elaeagnus pungens leaf oil are innovatively introduced and compounded with the salicylic acid, the calcium chloride and the 6-benayl aminopurine to form the plant growth regulator, and through the synergistic effect of various substances, on one hand, cell membranes and petal tissues can be protected against oxidative damage, and petal withering and discoloration are delayed; the flowers are helped to maintain physiological activities for a longer time; on the other hand, flower bud differentiation and flower development can be promoted, early flowering is promoted, and the flowering time is prolonged. The plant growth regulator effectively promotes early flowering of crape myrtle, Chinese rose and magnolia liliflora plants in gardens, prolongs the flowering period of flowers and improves the ornamental period of the flowers.