291 results about "Avian virus" patented technology

The invention belongs to the field of veterinary biotechnology detection, and particularly relates to a hybridoma cell strain and a monoclonal antibody for detecting or identifying MDV-1 and application of the hybridoma cell strain and the monoclonal antibody. The hybridoma cell strain MDV-1Mab-pp38-E2 is obtained through screening by taking pp38 protein of MDV-1 as immunogen, the preservation number is CCTCC NO: C202501, the monoclonal antibody generated by the hybridoma cell strain can recognize I-type chicken Marek's disease virus strains with different serum, and the monoclonal antibody can be used for identifying the type I chicken Marek's disease virus strains with different serum types. The kit has good specificity and sensitivity and can be used for exogenous virus detection of the serum type I chicken Marek's disease virus in poultry virus live vaccines without cross reaction with serum type II chicken Marek's disease virus, serum type III chicken Marek's disease virus, chicken egg drop syndrome virus (EDSV) and other common poultry disease viruses, and the kit has good specificity and sensitivity and can be applied to exogenous virus detection of the serum type I chicken Marek's disease virus in poultry virus live vaccines. The kit can also be used for clinical identification, virus content determination and epidemiological investigation of serotype I chicken Marek's disease virus.

The invention belongs to the technical field of biology, and particularly relates to a group of paired monoclonal antibodies for resisting H9N2 subtype avian influenza viruses and a surface enhanced Raman spectroscopy rapid detection test strip established based on the antibodies. The paired monoclonal antibody comprises a labeled antibody 1E9 and a capture antibody 3C10 which are prepared by taking hemagglutinin protein of the H9N2 subtype avian influenza virus as antigens and can be specifically combined with the H9N2 subtype avian influenza virus. According to the invention, an SERS (Surface Enhanced Raman Scattering) probe Au-coated Pt-4ATP is used as a marker and is coupled with a labeled antibody 1E9 to prepare a labeled probe, and the labeled probe is paired with a capture antibody 3C10 to establish an SERS immunochromatography test strip. The test strip has a colorimetric and Raman dual interpretation mode, can be used for qualitative and quantitative detection, has the advantages of strong specificity, high sensitivity, good repeatability and stability and the like, is simple and rapid in clinical detection operation, and provides reliable technical support for on-site rapid screening of subtype viruses.

The invention discloses a kit for visually detecting an H5 subtype avian influenza virus based on an RPA-CRISPR / Cas12b system and application of the kit, and belongs to the technical field of molecular detection. The kit comprises an RT-RPA (Reverse Transcription-Recombinase Polymerase Amplification) primer pair and sgRNA1 The RT-RPA primer pair comprises a forward primer with a nucleotide sequence as shown in SEQ ID NO.1 and a reverse primer with a nucleotide sequence as shown in SEQ ID NO.2; and the nucleotide sequence of the sgRNA1 is as shown in SEQ ID NO. 7. According to the kit, nucleic acid amplification and detection are synchronously completed through a tubular reaction system, the detection sensitivity reaches 1 copy / mu L, the coincidence rate with fluorescent RT-PCR is 100%, and no cross reaction exists on H7, H9, NDV and IBV. The kit does not need a complex instrument, is suitable for rapid screening on a basic level site, and provides an efficient solution for prevention and control of avian influenza.

The invention belongs to the field of biology, and discloses a monoclonal antibody of avian influenza virus PB2 protein, the monoclonal antibody comprises a heavy chain variable region and a light chain variable region, and can specifically bind to influenza virus matrix protein PB2, and the amino acid sequence of the heavy chain variable region is as shown in SEQ ID NO.1; the amino acid sequence of the light chain variable region is as shown in SEQ ID NO. 2; the hybridoma cell has relatively high sensitivity and specificity, and can be specifically combined with at least five subtype avian influenza PB2 proteins; meanwhile, the invention also provides application and drugs of the labeled compound, the hybridoma cell and the monoclonal antibody.

The invention belongs to the technical field of biology, and discloses a recombinant turkey herpesvirus co-expressing an avian influenza H9 gene and an avian adenovirus 4 type Fiber-2 gene, the H9 gene containing a promoter and a terminator is inserted between an HVT053 site and an HVT054 site of the turkey herpesvirus, the Fiber-2 gene containing a promoter and a terminator is inserted between an HVT087 site and an HVT088 site of the turkey herpesvirus, and the recombinant turkey herpesvirus co-expressing the avian influenza H9 gene and the avian adenovirus 4 type Fiber-2 gene is obtained. The recombinant virus is obtained. The recombinant turkey herpesvirus can stably co-express the H9N2 subtype avian influenza virus H9 protein and the fowl adenovirus 4 type Fiber-2 protein, has very excellent immune performance, and effectively improves the immune effect of the recombinant virus of the traditional fowl adenovirus Fiber-2 gene; compared with the recombinant herpesvirus of turkey which singly expresses any one of the H9N2 subtype avian influenza virus H9 protein and the fowl adenovirus 4 type Fiber-2 protein, the recombinant herpesvirus of turkey has the advantage that the performance of the recombinant herpesvirus of turkey is not weakened. Meanwhile, the invention also provides application and a vaccine of the recombinant herpesvirus of turkeys.

The invention discloses mRNA, an mRNA-LNP vaccine of the mRNA, a preparation method and application of the mRNA-LNP vaccine. The invention discloses an H5 subtype avian influenza virus vaccine, and aims to provide a vaccine which combines a mosaic sequence with an mRNA vaccine, achieves a good effect in an immune protection experiment of chickens, and can realize cross protection of branched strains of H5 subtype avian influenza virus 2.3. 4.4b and 2.3. 4.4 hours. According to the technical scheme, the nucleotide sequence of the mRNA is as shown in SEQ ID NO. 1; the nucleotide sequence of the mRNA is a Mosaic sequence which is obtained by designing a gene sequence of a 2.3. 4.4b branch strain of the avian influenza H5N6; the mRNA-LNP vaccine comprises mRNA (Messenger Ribonucleic Acid) with a nucleotide sequence as shown in SEQ ID NO. 1; belongs to the technical field of biology.

The invention discloses an avian influenza host bird behavior prediction method based on deep reinforcement learning, and the method comprises the following steps: S1, deploying a multi-angle and variable-frame-rate camera in a target habitat, and collecting multi-source video data; s2, performing frame insertion, illumination normalization and background denoising processing on the video data; s3, inputting the preprocessed data into an improved YOLOv5 model to obtain a target detection result; s4, constructing a dynamic graph neural network based on bird nodes and space-time edges, and outputting a clustering situation vector; s5, inputting the flight path into a low-layer Actor-Critic strategy network, and generating a local short-term behavior decision; s6, generating a global multi-walk prediction sequence in combination with the clustering situation vector and historical error feedback; and S7, comparing a prediction result with actual observation data, and triggering online fine adjustment according to deviation. According to the invention, non-intrusive and high-precision bird behavior prediction can be realized, and the method is suitable for bird flu monitoring and early warning scenes.

The invention discloses a primer probe group for simultaneously detecting H5, H7 and H9 subtype and universal avian influenza viruses. The kit comprises a probe of the H5 subtype avian influenza virus, an upstream primer of the H5 subtype avian influenza virus, a downstream primer of the H5 subtype avian influenza virus, a probe of the H7 subtype avian influenza virus, an upstream primer of the H7 subtype avian influenza virus, a downstream primer of the H7 subtype avian influenza virus, a probe of the H9 subtype avian influenza virus and an upstream primer of the H9 subtype avian influenza virus. The kit comprises an upstream primer of the H9 subtype avian influenza virus, a downstream primer of the H9 subtype avian influenza virus, a probe of the universal avian influenza virus, an upstream primer of the universal avian influenza virus and a downstream primer of the universal avian influenza virus. The invention also discloses a kit containing the primer probe group. The kit has the advantages of being simple and convenient to operate, high in sensitivity, good in specificity, capable of detecting samples anytime and anywhere, low in cross contamination rate, rapid in result obtaining and the like, suspicious samples can be found and diagnosed in time, and the rapid detection capacity of the avian influenza virus is improved.

The present invention uses ex vivo human airway cultures to assess the human transmissibility and replication competence of influenza and coronavirus strains. By comparing pandemic influenza A subtype H1N1 and highly pathogenic avian influenza H5N1 as reference strains, the transmissibility risk of various viruses was evaluated and categorized. Additionally, an in vitro model evaluated virus-induced impairment of alveolar fluid clearance (AFC) as an indicator of disease severity. The study revealed correlations between bronchus viral replication, human transmission, AFC impairment, and clinical disease severity across different influenza and coronavirus strains.

The invention provides an application of bortezomib in preparation of an anti-avian influenza virus drug. The bortezomib has the application of inhibiting the activity of avian influenza virus H7N9 neuraminidase. Researches show that the bortezomib can be combined with neuraminidase, the virtual calculation activity IC50 based on calculation is 3.37 * 10 <-7 > mol / L, the bortezomib can reduce the activity of neuraminidase, and the IC50 is 35.561 mu M; the bortezomib can inhibit the replication and propagation of avian influenza H7N9, and the IC50 is 0.043 [mu] M. In a word, the bortezomib has obvious activity of inhibiting neuraminidase of the avian influenza virus H7N9 and has the application of resisting the avian influenza virus.

The invention discloses an H9N2 subtype avian influenza virus, the H9N2 subtype avian influenza virus is A / chick / Guangxi / C1228 / 2015 (H9N2), and the preservation number of the H9N2 subtype avian influenza virus is CGMCC (China General Microbiological Culture Collection Center) No.45222; or the H9N2 subtype avian influenza virus is A / quil / Guangxi / 210Q33 / 2015 (H9N2), and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No. 46091. The bivalent inactivated vaccine of CK / GX / C1228 / 15 with better antigenicity and the strain QL / GX / 210Q33 / 15 which has better antigenicity and is separated from quails in the live poultry market has a better protection effect on two different branches of H9N2 subtype avian influenza viruses, the lowest immune dose (0.3 mL) can reach the protection rate of 80% or above, and the generated effective protection antibody can last to 24W.

The invention discloses a primer probe combination and a kit for on-site detection of H5, H7 and H9 subtype avian influenza viruses, and relates to the technical field of gene detection. The invention provides a primer probe combination for detecting H5, H7 and H9 subtype avian influenza viruses on site. The primer probe combination comprises a primer pair and a probe for detecting the H5 subtype avian influenza viruses, the primer pair and the probe are used for detecting the H7 subtype avian influenza virus; the invention discloses a primer pair and a probe for detecting an H9 subtype avian influenza virus. The method for detecting the H5, H7 and H9 subtype avian influenza viruses by adopting the primer probe combination and the kit disclosed by the invention integrates sample pretreatment, nucleic acid extraction, purification, amplification and detection, has better sensitivity and accuracy, is simple, convenient, rapid and time-saving to operate, and plays an important role in on-site rapid detection of research and safety management of the H5, H7 and H9 subtype avian influenza viruses.

The invention discloses a 2.3. 4.4b branch H5 subtype avian influenza virus strain and an application thereof. The 2.3. 4.4b branch H5 subtype avian influenza virus strain is an A type avian influenza virus A / chick / Shenyang / 12301 / 2024, is preserved in the China General Microbiological Culture Collection Center (CGMCC), has a preservation number of CGMCC No.46299, and is preserved for December 12, 2024, and the strain has a preservation number of CGMCC No.46299, a preservation number of CGMCC No.46299, a preservation number of CGMCC No.46299 and a preservation number of CGMCC No.46299. According to the invention, 2.3. 4.4b branch H5 subtype AIV with good cross immunoreaction is searched as a vaccine candidate strain to form a whole virus inactivated vaccine, so as to effectively prevent and control the infection of the latest epidemic H5 subtype AIV to poultry, and further eliminate the potential threat of influenza virus to public health safety in poultry breeding.

The invention provides a rotating machine fault diagnosis method for improving H5N1-VMD-MFWA noise reduction, and belongs to the technical field of mechanical fault diagnosis, and the method comprises the steps: obtaining vibration signals of a rotating machine under normal and fault conditions; taking the minimum envelope entropy as a fitness function, and performing parameter optimization on variational mode decomposition by adopting an improved avian influenza virus algorithm to obtain an optimal parameter [K, alpha] combination; variational mode decomposition is carried out on the vibration signal based on the optimal parameter, and K intrinsic mode function components are obtained through decomposition; and according to the correlation coefficient, the kurtosis and the envelope entropy of each component, a comprehensive evaluation index is constructed through a multi-feature weighted attention mechanism, each component is endowed with a weight, a signal is reconstructed, and the reconstructed signal is subjected to window segmentation and input into a convolutional neural network model to complete fault diagnosis. According to the method, the fault diagnosis accuracy is effectively improved, and a guarantee is provided for safe operation of mechanical equipment.

The invention belongs to the technical field of biology, and discloses an mRNA vaccine, and the mRNA vaccine is an mRNA vaccine encapsulated by LNP. The mRNA is designed according to the gene sequence of the avian influenza H5N6 strain, the mRNA vaccine disclosed by the invention is an mRNA vaccine obtained through LNP encapsulation, the vaccine can reach the action point more accurately through encapsulation, meanwhile, the mRNA part of the vaccine is designed according to the gene sequence of the avian influenza H5N6 strain, and in addition, the mRNA part of the vaccine can reach the action point more accurately. A challenge test shows that the mRNA vaccine not only has good humoral immunocompetence, but also has good cellular immunocompetence.

The invention relates to the technical field of avian virus detection, and particularly discloses a primer probe combination, a kit and a method for simultaneously detecting chicken infectious anemia virus and chicken circle virus type 3 based on fluorescent RAA and application. The method can be used for simultaneously detecting the chicken infectious anemia virus and the chicken circle virus type 3, and has the advantages of simplicity in operation, short detection time, good specificity, high sensitivity, no need of expensive instruments and equipment, support of on-site rapid detection, suitability for large-scale popularization and application and the like; and effective technical support can be provided for rapid detection and screening of the chicken infectious anemia virus and the chicken circle virus type 3.

This application relates to the field of virus detection technology, specifically disclosing a real-time fluorescent PCR detection kit and method for H10N7 avian influenza virus. The kit includes sample lysis extract, sample washing solution I, sample washing solution II, and reaction premix for detecting H10N7 subtype avian influenza virus; the detection method is an integrated detection method. This application achieves simultaneous detection of H10 and N7 subtype AIVs by designing specific primers and probes. The kit prepared using these primers and probes not only improves detection efficiency but also significantly enhances sensitivity and specificity. The combined use of MAOPA technology and real-time quantitative PCR technology further improves the accuracy of the detection results, making this kit of this application of significant application value in avian influenza prevention and control.

The invention provides an H9N2 avian influenza virus attenuated strain as well as a preparation method and application thereof, and belongs to the technical field of virus attenuation. The invention provides an attenuated strain of an H9N2 type avian influenza virus. The attenuated strain is obtained by modifying an M2 gene; the modification comprises the following steps: carrying out synonymous mutation on overlapped genes for coding the M1 protein and the M2 protein, replacing adjacent genes subjected to synonymous mutation with genes for coding termination codons, and carrying out deletion mutation on intracellular structural domain genes for coding the M2 protein. The H9N2 type avian influenza virus attenuated strain disclosed by the invention can be used for preparing an H9N2 type avian influenza virus attenuated vaccine. After chickens are immunized with the H9N2 avian influenza virus attenuated vaccine, a high-level hemagglutination inhibition antibody can be induced, no toxin is expelled after the chickens are immunized, safety is high, potential harm to the environment is avoided, and a new strategy is provided for prevention and control of H9N2 avian influenza.

The present invenon relates to a vaccine against influenza A viruses, said vaccine comprising HA protein and nanostructures bearing M2e, and its use for vaccinaon against avian influenza, especially in the veterinary field.

The invention discloses RT-qPCR-HRM (real-time quantitative polymerase chain reaction-high-resolution melting) detection primers and a detection method for identifying five different subtype avian influenza viruses, and belongs to the technical field of biology. The invention discloses an RT-qPCR-HRM detection method for identifying five different subtypes of avian influenza viruses, which comprises the following steps: designing a specific primer based on a highly conserved region of an AIV M gene, and simultaneously carrying out genetic typing on five subtypes of AIV strains H3, H5, H6, H7 and H9 in combination with an HRM technology; by comparing melting curve characteristics of M gene segments of different subtypes of AIV, an AIV detection method with good sensitivity and specificity is established. According to the method, the five AIVs of H3, H5, H6, H7 and H9 can be accurately typed, the defect of missing detection of a traditional AIV detection method is overcome, and the method has a wide application prospect.

The invention belongs to the field of biology, and relates to a broad-spectrum monoclonal antibody aiming at avian influenza virus M1 protein and application of the broad-spectrum monoclonal antibody, the monoclonal antibody has specific reaction with A549 cells infected by avian influenza, has no specific reaction with Newcastle disease virus, duck tembusu virus, goose astrovirus and infectious laryngitis virus, has good specificity, and can be used for preparing the broad-spectrum monoclonal antibody. The 5G9 monoclonal antibody has good reactivity with A549 cells infected by H1-H11 subtype AIV, has good broad spectrum, can be used for detecting M1 protein sub-localization after the AIV infected cells are detected by using the 5G9 monoclonal antibody, and can be used for indicating the infection process of the AIV.

The application belongs to the technical field of biology and particularly relates to an ELISA kit for detecting chicken astrovirus type II antibodies and a preparation method. The Cell-ELISA experiment is carried out by using LMH cells infected with chicken astrovirus type II to detect the chicken astrovirus type II antibodies. The method has no cross reactivity with positive serum of Newcastle disease virus, avian influenza virus, chicken infectious anemia virus, avian leukosis virus, Marek's virus and chicken infectious bursal virus, which indicates that the method has good specificity. The Cell-ELISA method is used to detect the chicken serum sample to be detected by using whole virus infection, and is assembled into a kit for measuring chicken astrovirus antibodies in serum. The kit can be used for serological diagnosis of chicken astrovirus type II infection, monitoring of the level of antibodies, epidemiological investigation and the like, and provides a novel and effective detection means for prevention and treatment of chicken astrovirus type II.

The present invention relates i.a. to a method for treating or preventing avian influenza virus infections in turkeys comprising administering an effective amount of a rHVT-H5HA COBRA.

The invention relates to construction and application of H7 subtype and H5 and H7 tetravalent avian influenza DNA vaccines. The invention belongs to the field of molecular biology, and aims to provide a DNA (deoxyribonucleic acid) vaccine for providing efficient immune protection for H7 subtype avian influenza virus. Specifically, the invention discloses an H7 subtype avian influenza virus hemagglutinin protein, which has an amino acid sequence as shown in SEQ ID NO: 5 and / or SEQ ID NO: 6. The invention also discloses polynucleotide for coding the protein, and the polynucleotide has a sequence as shown in SEQ ID NO: 1 and / or SEQ ID NO: 2. The polynucleotide can be used for preparing a vaccine aiming at the current epidemic H7 subtype avian influenza virus. The H5 + H7 tetravalent DNA vaccine is obtained by combining the vaccine with a vaccine aiming at the H5 subtype, and the H5 + H7 tetravalent DNA vaccine can be used for prevention and control of current H5 subtype and H7 subtype avian influenza.

The present application relates to the field of virus detection technology, specifically disclosing a dual microfluidic fluorescent PCR detection kit for the H3N2 subtype of avian influenza virus. The dual microfluidic fluorescent PCR detection kit comprises a sample lysis extract for detecting the H3N2 subtype of avian influenza virus, a sample wash solution I, a sample wash solution II, and a reaction premix. The reaction premix contains three sets of specific sequence combinations: an H3-specific sequence combination, an N2-specific sequence combination, and an internal standard gene-specific sequence combination. By designing specific primers and probes, and performing optimization and screening, the present application enables simultaneous identification of H3 and N2 subtype AIVs in a single reaction. Furthermore, the amplification reactions of the two target genes are free of interference, resulting in high specificity, high sensitivity, and good reproducibility. This kit enables accurate diagnosis of viral infection and has important application value in the prevention and control of avian influenza epidemics.

The invention uses ex vivo human airway cultures to assess the transmissibility and replication ability of influenza and coronavirus strains. By comparing the pandemic influenza A H1N1 and the highly pathogenic influenza A H5N1 as reference virus strains, the spreading risks of various viruses are evaluated and classified. In addition, an in vitro model is used to assess the degree of alveolar fluid clearance (AFC) impairment caused by the virus as an indicator of the severity of the disease. The research reveals the relevance among the virus replication ability, human transmission, AFC damage degree and clinical disease severity of different influenza and coronavirus strains in bronchus.

The invention relates to the field of animal health. In particular, the present invention relates to a composition comprising a modified duck enteritis virus (DEV) comprising and capable of expressing a heterologous polynucleotide encoding an antigen of a chicken pathogen. In addition, the present invention relates to a composition comprising the modified DEV as a chicken vector vaccine and uses thereof. More particularly, the present invention relates to a vaccine composition against chicken pathogens comprising a modified DEV that does not exhibit or exhibits reduced pathogenicity in chicken.

The invention belongs to the technical field of biology, and discloses a TaqMan primer probe combination for detecting virulent and attenuated Newcastle disease, which comprises a virulent primer RT, a virulent probe RT-probe, a universal primer SRT and a universal probe SRT-probe, the virulent primer RT comprises an upstream primer RT-F and a downstream primer RT-R, and the universal primer SRT comprises an upstream primer SRT-F and a downstream primer SRT-R; a TaqMan probe fluorescent quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection method for virulent and attenuated NDV (Newcastle Disease Virus) established by using the TaqMan primer probe combination has no cross reaction on amplification of common avian viruses AIV, FADV, IBV and IBDV and has good specificity; the lowest detection concentration of virulent and attenuated Newcastle disease virus is 101 copies / uL, which is 100 times higher than that of a conventional RT-PCR method, and the kit has good sensitivity; and compared with the conventional RT-PCR method, the detection rate is 100% and is superior to that of the conventional RT-PCR method, and the detection result is consistent with the sequencing result.

The present disclosure is directed to antibodies binding to and neutralizing avian H5N1 influenza virus and methods for use thereof.