244 results about "Avian virus" patented technology

The invention belongs to the technical field of biology, and particularly relates to a group of paired monoclonal antibodies for resisting H9N2 subtype avian influenza viruses and a surface enhanced Raman spectroscopy rapid detection test strip established based on the antibodies. The paired monoclonal antibody comprises a labeled antibody 1E9 and a capture antibody 3C10 which are prepared by taking hemagglutinin protein of the H9N2 subtype avian influenza virus as antigens and can be specifically combined with the H9N2 subtype avian influenza virus. According to the invention, an SERS (Surface Enhanced Raman Scattering) probe Au-coated Pt-4ATP is used as a marker and is coupled with a labeled antibody 1E9 to prepare a labeled probe, and the labeled probe is paired with a capture antibody 3C10 to establish an SERS immunochromatography test strip. The test strip has a colorimetric and Raman dual interpretation mode, can be used for qualitative and quantitative detection, has the advantages of strong specificity, high sensitivity, good repeatability and stability and the like, is simple and rapid in clinical detection operation, and provides reliable technical support for on-site rapid screening of subtype viruses.

The invention discloses a kit for visually detecting an H5 subtype avian influenza virus based on an RPA-CRISPR / Cas12b system and application of the kit, and belongs to the technical field of molecular detection. The kit comprises an RT-RPA (Reverse Transcription-Recombinase Polymerase Amplification) primer pair and sgRNA1 The RT-RPA primer pair comprises a forward primer with a nucleotide sequence as shown in SEQ ID NO.1 and a reverse primer with a nucleotide sequence as shown in SEQ ID NO.2; and the nucleotide sequence of the sgRNA1 is as shown in SEQ ID NO. 7. According to the kit, nucleic acid amplification and detection are synchronously completed through a tubular reaction system, the detection sensitivity reaches 1 copy / mu L, the coincidence rate with fluorescent RT-PCR is 100%, and no cross reaction exists on H7, H9, NDV and IBV. The kit does not need a complex instrument, is suitable for rapid screening on a basic level site, and provides an efficient solution for prevention and control of avian influenza.

The invention discloses an avian influenza host bird behavior prediction method based on deep reinforcement learning, and the method comprises the following steps: S1, deploying a multi-angle and variable-frame-rate camera in a target habitat, and collecting multi-source video data; s2, performing frame insertion, illumination normalization and background denoising processing on the video data; s3, inputting the preprocessed data into an improved YOLOv5 model to obtain a target detection result; s4, constructing a dynamic graph neural network based on bird nodes and space-time edges, and outputting a clustering situation vector; s5, inputting the flight path into a low-layer Actor-Critic strategy network, and generating a local short-term behavior decision; s6, generating a global multi-walk prediction sequence in combination with the clustering situation vector and historical error feedback; and S7, comparing a prediction result with actual observation data, and triggering online fine adjustment according to deviation. According to the invention, non-intrusive and high-precision bird behavior prediction can be realized, and the method is suitable for bird flu monitoring and early warning scenes.

The present invention uses ex vivo human airway cultures to assess the human transmissibility and replication competence of influenza and coronavirus strains. By comparing pandemic influenza A subtype H1N1 and highly pathogenic avian influenza H5N1 as reference strains, the transmissibility risk of various viruses was evaluated and categorized. Additionally, an in vitro model evaluated virus-induced impairment of alveolar fluid clearance (AFC) as an indicator of disease severity. The study revealed correlations between bronchus viral replication, human transmission, AFC impairment, and clinical disease severity across different influenza and coronavirus strains.

The invention discloses a primer probe combination and a kit for on-site detection of H5, H7 and H9 subtype avian influenza viruses, and relates to the technical field of gene detection. The invention provides a primer probe combination for detecting H5, H7 and H9 subtype avian influenza viruses on site. The primer probe combination comprises a primer pair and a probe for detecting the H5 subtype avian influenza viruses, the primer pair and the probe are used for detecting the H7 subtype avian influenza virus; the invention discloses a primer pair and a probe for detecting an H9 subtype avian influenza virus. The method for detecting the H5, H7 and H9 subtype avian influenza viruses by adopting the primer probe combination and the kit disclosed by the invention integrates sample pretreatment, nucleic acid extraction, purification, amplification and detection, has better sensitivity and accuracy, is simple, convenient, rapid and time-saving to operate, and plays an important role in on-site rapid detection of research and safety management of the H5, H7 and H9 subtype avian influenza viruses.

The invention provides a rotating machine fault diagnosis method for improving H5N1-VMD-MFWA noise reduction, and belongs to the technical field of mechanical fault diagnosis, and the method comprises the steps: obtaining vibration signals of a rotating machine under normal and fault conditions; taking the minimum envelope entropy as a fitness function, and performing parameter optimization on variational mode decomposition by adopting an improved avian influenza virus algorithm to obtain an optimal parameter [K, alpha] combination; variational mode decomposition is carried out on the vibration signal based on the optimal parameter, and K intrinsic mode function components are obtained through decomposition; and according to the correlation coefficient, the kurtosis and the envelope entropy of each component, a comprehensive evaluation index is constructed through a multi-feature weighted attention mechanism, each component is endowed with a weight, a signal is reconstructed, and the reconstructed signal is subjected to window segmentation and input into a convolutional neural network model to complete fault diagnosis. According to the method, the fault diagnosis accuracy is effectively improved, and a guarantee is provided for safe operation of mechanical equipment.

The invention relates to the technical field of avian virus detection, and particularly discloses a primer probe combination, a kit and a method for simultaneously detecting chicken infectious anemia virus and chicken circle virus type 3 based on fluorescent RAA and application. The method can be used for simultaneously detecting the chicken infectious anemia virus and the chicken circle virus type 3, and has the advantages of simplicity in operation, short detection time, good specificity, high sensitivity, no need of expensive instruments and equipment, support of on-site rapid detection, suitability for large-scale popularization and application and the like; and effective technical support can be provided for rapid detection and screening of the chicken infectious anemia virus and the chicken circle virus type 3.

This application relates to the field of virus detection technology, specifically disclosing a real-time fluorescent PCR detection kit and method for H10N7 avian influenza virus. The kit includes sample lysis extract, sample washing solution I, sample washing solution II, and reaction premix for detecting H10N7 subtype avian influenza virus; the detection method is an integrated detection method. This application achieves simultaneous detection of H10 and N7 subtype AIVs by designing specific primers and probes. The kit prepared using these primers and probes not only improves detection efficiency but also significantly enhances sensitivity and specificity. The combined use of MAOPA technology and real-time quantitative PCR technology further improves the accuracy of the detection results, making this kit of this application of significant application value in avian influenza prevention and control.

The invention discloses RT-qPCR-HRM (real-time quantitative polymerase chain reaction-high-resolution melting) detection primers and a detection method for identifying five different subtype avian influenza viruses, and belongs to the technical field of biology. The invention discloses an RT-qPCR-HRM detection method for identifying five different subtypes of avian influenza viruses, which comprises the following steps: designing a specific primer based on a highly conserved region of an AIV M gene, and simultaneously carrying out genetic typing on five subtypes of AIV strains H3, H5, H6, H7 and H9 in combination with an HRM technology; by comparing melting curve characteristics of M gene segments of different subtypes of AIV, an AIV detection method with good sensitivity and specificity is established. According to the method, the five AIVs of H3, H5, H6, H7 and H9 can be accurately typed, the defect of missing detection of a traditional AIV detection method is overcome, and the method has a wide application prospect.

The invention belongs to the field of biology, and relates to a broad-spectrum monoclonal antibody aiming at avian influenza virus M1 protein and application of the broad-spectrum monoclonal antibody, the monoclonal antibody has specific reaction with A549 cells infected by avian influenza, has no specific reaction with Newcastle disease virus, duck tembusu virus, goose astrovirus and infectious laryngitis virus, has good specificity, and can be used for preparing the broad-spectrum monoclonal antibody. The 5G9 monoclonal antibody has good reactivity with A549 cells infected by H1-H11 subtype AIV, has good broad spectrum, can be used for detecting M1 protein sub-localization after the AIV infected cells are detected by using the 5G9 monoclonal antibody, and can be used for indicating the infection process of the AIV.

The application belongs to the technical field of biology and particularly relates to an ELISA kit for detecting chicken astrovirus type II antibodies and a preparation method. The Cell-ELISA experiment is carried out by using LMH cells infected with chicken astrovirus type II to detect the chicken astrovirus type II antibodies. The method has no cross reactivity with positive serum of Newcastle disease virus, avian influenza virus, chicken infectious anemia virus, avian leukosis virus, Marek's virus and chicken infectious bursal virus, which indicates that the method has good specificity. The Cell-ELISA method is used to detect the chicken serum sample to be detected by using whole virus infection, and is assembled into a kit for measuring chicken astrovirus antibodies in serum. The kit can be used for serological diagnosis of chicken astrovirus type II infection, monitoring of the level of antibodies, epidemiological investigation and the like, and provides a novel and effective detection means for prevention and treatment of chicken astrovirus type II.

The present invention relates i.a. to a method for treating or preventing avian influenza virus infections in turkeys comprising administering an effective amount of a rHVT-H5HA COBRA.

The invention relates to construction and application of H7 subtype and H5 and H7 tetravalent avian influenza DNA vaccines. The invention belongs to the field of molecular biology, and aims to provide a DNA (deoxyribonucleic acid) vaccine for providing efficient immune protection for H7 subtype avian influenza virus. Specifically, the invention discloses an H7 subtype avian influenza virus hemagglutinin protein, which has an amino acid sequence as shown in SEQ ID NO: 5 and / or SEQ ID NO: 6. The invention also discloses polynucleotide for coding the protein, and the polynucleotide has a sequence as shown in SEQ ID NO: 1 and / or SEQ ID NO: 2. The polynucleotide can be used for preparing a vaccine aiming at the current epidemic H7 subtype avian influenza virus. The H5 + H7 tetravalent DNA vaccine is obtained by combining the vaccine with a vaccine aiming at the H5 subtype, and the H5 + H7 tetravalent DNA vaccine can be used for prevention and control of current H5 subtype and H7 subtype avian influenza.

The present application relates to the field of virus detection technology, specifically disclosing a dual microfluidic fluorescent PCR detection kit for the H3N2 subtype of avian influenza virus. The dual microfluidic fluorescent PCR detection kit comprises a sample lysis extract for detecting the H3N2 subtype of avian influenza virus, a sample wash solution I, a sample wash solution II, and a reaction premix. The reaction premix contains three sets of specific sequence combinations: an H3-specific sequence combination, an N2-specific sequence combination, and an internal standard gene-specific sequence combination. By designing specific primers and probes, and performing optimization and screening, the present application enables simultaneous identification of H3 and N2 subtype AIVs in a single reaction. Furthermore, the amplification reactions of the two target genes are free of interference, resulting in high specificity, high sensitivity, and good reproducibility. This kit enables accurate diagnosis of viral infection and has important application value in the prevention and control of avian influenza epidemics.

The invention uses ex vivo human airway cultures to assess the transmissibility and replication ability of influenza and coronavirus strains. By comparing the pandemic influenza A H1N1 and the highly pathogenic influenza A H5N1 as reference virus strains, the spreading risks of various viruses are evaluated and classified. In addition, an in vitro model is used to assess the degree of alveolar fluid clearance (AFC) impairment caused by the virus as an indicator of the severity of the disease. The research reveals the relevance among the virus replication ability, human transmission, AFC damage degree and clinical disease severity of different influenza and coronavirus strains in bronchus.

The invention relates to the field of animal health. In particular, the present invention relates to a composition comprising a modified duck enteritis virus (DEV) comprising and capable of expressing a heterologous polynucleotide encoding an antigen of a chicken pathogen. In addition, the present invention relates to a composition comprising the modified DEV as a chicken vector vaccine and uses thereof. More particularly, the present invention relates to a vaccine composition against chicken pathogens comprising a modified DEV that does not exhibit or exhibits reduced pathogenicity in chicken.

The invention belongs to the technical field of biology, and discloses a TaqMan primer probe combination for detecting virulent and attenuated Newcastle disease, which comprises a virulent primer RT, a virulent probe RT-probe, a universal primer SRT and a universal probe SRT-probe, the virulent primer RT comprises an upstream primer RT-F and a downstream primer RT-R, and the universal primer SRT comprises an upstream primer SRT-F and a downstream primer SRT-R; a TaqMan probe fluorescent quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection method for virulent and attenuated NDV (Newcastle Disease Virus) established by using the TaqMan primer probe combination has no cross reaction on amplification of common avian viruses AIV, FADV, IBV and IBDV and has good specificity; the lowest detection concentration of virulent and attenuated Newcastle disease virus is 101 copies / uL, which is 100 times higher than that of a conventional RT-PCR method, and the kit has good sensitivity; and compared with the conventional RT-PCR method, the detection rate is 100% and is superior to that of the conventional RT-PCR method, and the detection result is consistent with the sequencing result.

The present disclosure is directed to antibodies binding to and neutralizing avian H5N1 influenza virus and methods for use thereof.

The invention belongs to the technical field of virus detection, and particularly relates to a primer composition for detecting an avian influenza NA gene, a detection method and a detection device. The avian influenza NA genes comprise NA genes of an influenza A virus H5N1, an influenza A virus H5N6, an influenza A virus H7N7, an influenza A virus H7N9 and an influenza A virus H9N2, and the primer composition comprises nucleotide sequences as shown in SEQ ID No.1-25. The primer composition for detecting the NA gene causing the highly pathogenic human and livestock co-infected avian influenza, provided by the invention, can be used for detecting whether a sample contains the NA gene causing the highly pathogenic human and livestock co-infected avian influenza, namely NA genes of influenza A virus subtypes H5N1, H5N6, H7N7, H7N9 and H9N2, and a result can be conveniently, quickly and accurately detected; in a practical application process, a detection result can be obtained within 40 minutes.

This invention relates to a method and its application for determining the plasmid content in a quadrivalent avian influenza DNA vaccine. Belonging to the field of molecular biology, this invention aims to provide a TaqMan quantitative real-time PCR method capable of quantitatively determining the content of each plasmid component in a quadrivalent DNA vaccine. Specifically, this invention provides a primer and probe composition for detecting the content of individual plasmids in a quadrivalent avian influenza (H5+H7) DNA vaccine based on TaqMan quantitative real-time PCR. The composition includes primers with nucleotide sequences shown in SEQ ID NO: 5-6, 8-9, 11-12, and 14-15, and TaqMan probes with nucleotide sequences shown in SEQ ID NO: 7, 10, 13, and 16. This primer and probe composition can quantitatively determine the content of each plasmid component in the quadrivalent DNA vaccine, exhibiting good specificity and reproducibility.

The utility model provides a kind of for preventing avian influenza's medicine spraying device, belong to spraying device field, including base, medicine jar, rotating shaft, big bevel gear, motor, water pump and spraying assembly, medicine jar and water pump are respectively arranged on base, and one end of water pump is connected with the bottom of medicine jar one side, the other end of water pump is connected with spraying assembly, the top of medicine jar is provided with jar cover, rotating shaft rotation is set in the middle part of jar cover, big bevel gear is set on one end of rotating shaft, the other end of rotating shaft extends to the inside of medicine jar, rotating shaft in medicine jar inside is intervally provided with several clean water spray head and cleaning assembly, rotating shaft is set as hollow shaft, motor is set in the top of jar cover one side, the output of motor is provided with small bevel gear, small bevel gear is engaged with big bevel gear, the bottom end of base is respectively provided with self-locking universal wheel.The utility model is cleaned by the cleaning assembly and clean water spray head of rotating shaft to the medicine in medicine jar wall residual drug.

The invention relates to the technical field of molecular biology. The invention provides a primer probe combination for detecting an H10 subtype avian influenza virus, the primer probe combination comprises a primer pair and a probe for detecting the H10 subtype avian influenza virus, and the primer pair for detecting the H10 subtype avian influenza virus comprises an H10-F sequence as shown in SEQ ID NO.1 and an H10-R sequence as shown in SEQ ID NO.2; and the probe for detecting the H10 subtype avian influenza virus is an H10-P sequence as shown in SEQ ID NO.3. The invention further discloses a kit for detecting the H10 subtype avian influenza virus. The primer probe combination disclosed by the invention can be used for detecting and distinguishing epidemic H10 viruses of European subpedigree and North American pedigree, does not generate cross reaction with other common viruses, and is high in detection sensitivity, and the minimum detectable quantity is 5 copies.

The present invention provides recombinant herpes virus of turkeys (HVT) viral vectors comprising a heterologous polynucleotide encoding an avian influenza antigen. The recombinant viral vectors are suitable for use in immunogenic compositions and vaccines, and can provide protection against avian influenza and other pathogens when administered to an avian.

The application discloses a chicken Newcastle disease, avian influenza and chicken infectious rhinitis triple inactivated vaccine and preparation and application thereof, and relates to the technical field of bioengineering. The application discloses a preparation method and application of a chicken Newcastle disease, avian influenza and chicken infectious rhinitis (A, B and C three serotypes) subunit protein triple inactivated vaccine. The preparation method comprises the following steps: constructing and expressing a serum B paracolon avian bacillus subunit protein expression strain; and preparing the chicken Newcastle disease, H9 subtype avian influenza and chicken infectious rhinitis (A, B and C three serotypes) subunit protein triple inactivated vaccine at a certain ratio. The triple inactivated vaccine has good safety, can be used for the prevention of chicken Newcastle disease, H9 subtype avian influenza and A, B and C three serotypes paracolon avian bacillus diseases in a laying hen group, and does not cause the decrease of the laying rate after immunization. Meanwhile, the paracolon avian bacillus subunit protein can improve the antibody level of the Newcastle disease and the H9 avian influenza, and the protection of the vaccine on the A, B and C three serotypes paracolon avian bacillus can reach 100%.

The invention discloses a recombinant serum 4 type fowl adenovirus FAdV4-IBV-QXS1-rf2 carrying an infectious bronchitis S1 gene, wherein a gene locus of the serum 4 type fowl adenovirus Fiber-2 is replaced by an S1 protein gene of an infectious bronchitis virus QX subtype. According to the FAdV4-IBV-QXS1-rf2 recombinant virus, the CRISPR / cas9 technology is used for gene editing of the serum type 4 fowl adenovirus, the pathogenicity of the virus is reduced, meanwhile, high immunogenicity is reserved, and the FAdV4-IBV-QXS1-rf2 recombinant virus which is high in purity and can be inherited stably is obtained. The recombinant virus can activate mucous membrane and humoral immunity of chicken, achieves a dual immune protection effect on FAdV-4 and IBV-QX infection, overcomes the defect of single targeting of traditional vaccines, and provides a general technical path for research and development of multi-combined vaccines of avian influenza, Newcastle disease and other poultry viruses.

The application discloses a kind of avian influenza and avian infectious bronchitis two-union recombinant subunit vaccine and its preparation method and application.The recombinant protein is by replacing the head domain of AIV HA protein with the RBD domain of IBV S protein, while adding GCN4, Trimer-tag trimer tag in sequence, the recombinant protein obtained, the recombinant protein is expressed in vitro using insect cells and prepared into subunit vaccine.The vaccine prepared by the application can produce high-level neutralizing antibodies against homologous and heterologous H9N2 subtype avian influenza virus after immunizing chicken population, while significantly reducing the virus discharge level and the histopathological changes caused, and can also induce high-level specific antibodies and neutralizing antibodies against QX subtype avian infectious bronchitis virus, effectively inhibit discharge, viral load, and reduce tissue lesions.The application does not depend on chicken embryo, and has low production cost and short cycle, and can simultaneously prevent avian influenza and avian infectious bronchitis.

The application belongs to the technical field of nucleic acid detection methods, and particularly relates to a rapid detection method of avian influenza virus, which comprises using a primer pair and a probe designed for a conserved region of M gene of AIV, and amplifying amplification reagents containing a sample to be detected based on super-fast qPCR technology, wherein the sequence of a forward primer in the primer pair is 5'-GGCCCCCTCAAAGCCGA-3'; the sequence of a reverse primer is 5'-CGTCTACGCTGCAGTCC-3'; and the sequence of the probe is ROX-CACTGGGCACGGTGAGCGT-BHQ2. The method of the application can rapidly and accurately detect whether the avian influenza virus exists in the sample to be detected and the content of the avian influenza virus, and has a good application prospect.

The invention belongs to the technical field of biological medicines, and particularly relates to a compound protein preparation for preventing and treating avian influenza and a preparation method thereof. The compound protein preparation is prepared from the following raw materials in parts by weight: 5 to 10 parts of immune globulin, 15 to 19 parts of radix puerariae, 10 to 15 parts of buchnera macrocarpa, 8 to 12 parts of dendranthema morifolium, 7 to 10 parts of honeysuckle flower, 3 to 10 parts of radix astragali seu hedysari, 2 to 8 parts of echinacea purpurea, 1 to 7 parts of semen cuscutae and 2 to 5 parts of liquorice root. By taking the compound protein preparation for preventing and treating the avian influenza, clinical symptoms caused by the avian influenza can be effectively relieved, and the health level of animals is improved; the vaccine-induced immune response can be effectively enhanced, so that the avian influenza antibody level of animals is improved, the avian influenza is effectively prevented and treated, and the vaccine has a wide application prospect.

The invention provides application of M1 protein related substances in preparation of medicines for treating diseases caused by avian influenza viruses. The application is any one of the following applications: application of Y1 in preparation of medicines for preventing and / or treating diseases caused by avian influenza viruses or avian influenza virus infection; the Y2 is applied to preparation of an avian influenza virus inhibitor; the invention discloses application of Y3 in inhibition of avian influenza virus replication. After a mouse is immunized by the M1 recombinant protein, immune factors related to T cells are increased, secretion of proinflammatory factors is reduced, preparation is made for protecting a body from virus attack, and the M1 recombinant protein is also an expression for laterally improving the immunity of a host. The M1 protein has a good immune effect as an immunogen. The invention has application value in treatment of avian influenza virus and similar viruses.