16 results about "Chicken serum" patented technology

The application belongs to the technical field of biology and particularly relates to an ELISA kit for detecting chicken astrovirus type II antibodies and a preparation method. The Cell-ELISA experiment is carried out by using LMH cells infected with chicken astrovirus type II to detect the chicken astrovirus type II antibodies. The method has no cross reactivity with positive serum of Newcastle disease virus, avian influenza virus, chicken infectious anemia virus, avian leukosis virus, Marek's virus and chicken infectious bursal virus, which indicates that the method has good specificity. The Cell-ELISA method is used to detect the chicken serum sample to be detected by using whole virus infection, and is assembled into a kit for measuring chicken astrovirus antibodies in serum. The kit can be used for serological diagnosis of chicken astrovirus type II infection, monitoring of the level of antibodies, epidemiological investigation and the like, and provides a novel and effective detection means for prevention and treatment of chicken astrovirus type II.

The invention discloses a system for directionally differentiating chicken primordial germ cells into gametes in vitro and a regulation and control method, the system comprises a basic culture medium: based on DMEM / F12, adding 10% of fetal calf serum, 2% of chicken serum, 2 mmol / L of L-glutamine, 1 mmol / L of sodium pyruvate and 5.5 * 10 <-5 > mol / L of beta-mercaptoethanol; the spermatogonium induction culture medium further comprises BMP4, RA and GDNF; the oogonium induction culture medium also comprises BMP15, GDF9 and EGF (Epidermal Growth Factor). According to the system and the regulation and control method, the differentiation efficiency is improved, the culture period is shortened, and the number of cell clusters is remarkably increased.

The utility model relates to the technical field of production of chicken drops, in particular to a high-temperature and high-pressure sterilization device for chicken drops, which comprises a base, a sterilization tank and a hot water tank are mounted at the top of the base, and a drainage pipe for draining waste water is arranged at the bottom of the sterilization tank; the filtering mechanism comprises a filtering box which is fixedly connected to the end, away from the sterilization tank, of the drainage pipe, a filtering net is arranged in the filtering box, and an arc-shaped baffle is fixedly connected to the interior of the filtering box. The waste heat in the waste water is collected and utilized, the heated water body is supplemented into the hot water tank, heat waste is avoided, when the waste water in the sterilization tank is discharged, impurities in the waste water are filtered through the filter screen in the filter tank, residues of the impurities in the waste water are reduced, and follow-up waste water reutilization is facilitated.

The invention relates to the technical field of traditional Chinese medicine composition processing, in particular to a compound traditional Chinese medicine composition for improving immunity of poultry and a preparation method of the composition. Aiming at the physiological characteristics of short digestive tract and short food absorption time of the poultry, a basic formula with qi tonifying as a main part and digestion promoting as an assistant part is developed; a new composition is developed by optimizing a self-created basic prescription through technical means such as flavoring, odor reduction and substitution, so that the immune function is remarkably improved, the double effects of effectively reducing the standard deviation of chicken serum IgG and reducing the dispersion degree of data in a group are achieved, the overall improvement of group immunity is achieved, the immune effect of vaccines can be enhanced, and the application prospect is wide. Meanwhile, the dosage of the improved prescription is reduced by 33.3% compared with that of the basic prescription, the medication cost is reduced, in addition, by optimizing the polysaccharide water extraction process, the polysaccharide alcohol extraction process is effectively replaced, the problems that an alcohol extraction method is large in pollution, expensive in equipment and the like are solved, and the application and popularization capacity of the prescription is effectively improved.

The invention provides two specific diagnostic antigens for chicken infected with dog head roundworm and an ELISA diagnostic kit thereof, and belongs to the technical field of biological immunodetection, and the two specific diagnostic antigens are a recombinant protein GAPDH3 and a recombinant protein dim-1; the nucleotide sequence of the specific diagnosis antigen gene GAPDH3 is as shown in SEQ ID NO.1, and the amino acid sequence of the specific diagnosis antigen gene GAPDH3 is as shown in SEQ ID NO.3; the nucleotide sequence of the specific diagnosis antigen gene dim-1 is as shown in SEQ ID NO.2, and the amino acid sequence of the specific diagnosis antigen gene dim-1 is as shown in SEQ ID NO.4. The kit is used for detecting the specific antibody of the dog head roundworm in chicken serum, detection signals can be displayed more clearly in the detection process, misjudgment caused by non-specific reaction is reduced, and the detection accuracy and reliability are improved. The condition of the specific antibody of the dog head roundworm in chicken serum can be more accurately judged, and a more reliable basis is provided for diagnosis of chicken infection of the dog head roundworm.

The invention provides application of an organic iron source in preparation of feed or medicine for relieving chicken eimeria tenella disease, and relates to the technical field of feed additives and veterinary drugs. The organic iron source comprises one of a soybean protein iron chelate, a glycine iron complex and a methionine iron chelate. The feed or the medicine has at least one of the following functions: (1) the content of immune globulins IgA, IgG and IgM in chicken serum infected by eimeria tenella is increased; (2) reducing the expression level of the appendicular factors of the appendicular factors after the appendicular factors are infected by the eimeria tenella, wherein the appendicular factors of the appendicular factors comprise IL-1beta, IL-8, TNF-alpha and IFN-gamma; (3) the death rate of the chicken infected by eimeria tenella is reduced; (4) increasing the weight of the chicken infected by eimeria tenella; and (5) reducing the cecum lesion score of the chicken infected by the eimeria tenella.

The application discloses a chicken interleukin 2 antibody and application thereof, the antibody comprises a light chain and a heavy chain variable region, the amino acid sequences of three complementarity determining regions in the light chain variable region are respectively shown as SEQ ID NO. 1-3, the amino acid sequences of three complementarity determining regions in the heavy chain variable region are respectively shown as SEQ ID NO. 5-7, or the amino acid sequences of three complementarity determining regions in the light chain variable region are respectively shown as SEQ ID NO. 17-19, and the amino acid sequences of three complementarity determining regions in the heavy chain variable region are respectively shown as SEQ ID NO. 21-23. The antibody provided by the application has the advantages of high effective value, good specificity, high affinity and reaction with natural ChIL-2 antigen. The ChIL-2 double antibody sandwich enzyme-linked immunosorbent assay (ELISA) established based on the two antibodies has the advantages of high specificity, high sensitivity, simple operation and good repeatability, and can be used for determination of the ChIL-2 content in cell culture supernatant, chicken serum and other samples.

The application provides application of an IGFBP1 gene in regulation or evaluation of hen reproductive performance, and belongs to the field of biotechnology and genetic breeding. The application promotes expression of the IGFBP1 gene of a hen by constructing a tissue-specific IGFBP1 recombinant adeno-associated virus vector and using the tissue-specific IGFBP1 recombinant adeno-associated virus vector to promote expression of the IGFBP1 gene of the hen, and improves the IGFBP1 protein content in serum of the hen, and further improves the reproductive performance of the hen. The application proves by experiments that the IGFBP1 gene has an influence on the egg production performance of a hen and the expression level of an HPG axis hormone, can improve the total egg production of a hen, an ovary index, the number of small white follicles, the number of large white follicles, and the content of LH, E2, AMH and FSH in serum, can be used as a candidate gene or a genetic marker of egg production performance of a hen, enriches the serum judgment index of hen reproductive performance, and provides a valuable reference basis for differential diagnosis of hen reproductive performance.

Disclosed are a chicken serum-free culture medium for promoting the proliferation of chicken primordial germ cells and use thereof. Ovotransferrin is used in the culture medium instead of chicken serum. The effect of using ovotransferrin, instead of chicken serum, in a culture system for PGCs is disclosed. Ovotransferrin can maintain the self-renewal of PGCs, thereby providing a basis for developing a stable culture system. The culture medium can avoid adverse effects caused by the undefined components of chicken serum, and the precise use of various growth factors can provide better culture conditions and reduce culture costs.

The present application relates to the technical field of molecular marker, and particularly relates to a SNP molecular marker associated with chicken bile acid traits, a primer group and application. The molecular marker is located at the 641bp of the nucleotide sequence shown in SEQ ID No. 1, and the base at the position has A / G polymorphism. The molecular marker provided by the present application is closely associated with the bile acid level of chicken. Compared with the chicken with the genotype AG / GG at the site, the chicken with the genotype AA has a higher bile acid level in serum or bile, and the chicken excrement has a lower bile acid level. The molecular marker can be applied to assist in breeding high-quality chicken with high fat utilization rate, low protein loss and high protein content, thereby effectively improving the economic benefits of raising chicken.

The utility model relates to the technical field of drop chicken serum stock solution production, concretely relates to a kind of for drop chicken serum stock solution cooking device, it include: frame, the frame top is provided with top cover, the frame inside is provided with two symmetrical distribution's high-pressure cooking pot;Lifting mechanism, including fixedly connected in the drive motor of top cover outside, the drive motor is fixedly connected with threaded rod by output shaft, the threaded rod outside is connected with internal thread plate, the internal thread plate outside is equipped with winch, the utility model is taken out from high-pressure cooking pot when material, along with the contraction of rope, when net box is removed from high-pressure cooking pot, when round block enters into round pipe, under the location of round block and round pipe, net box is not easy to sway under the action of rope when moving, effectively prevent the spilling of drop chicken serum stock solution inside tray, avoid the waste of drop chicken serum stock solution when hoisting.

The application discloses a low-stress light supplementing method of a chicken house inspection robot. The method comprises the following steps: installing a cool white light source on the inspection robot in the chicken house; and when the inspection robot is used to inspect each cage in the chicken house, the cool white light source is used to irradiate the chickens in each cage passed by the inspection robot in sequence to supplement the light of the chickens with low stress. The method can improve the levels of chicken serum glutamine, chicken superoxide dismutase, chicken corticosterone, chicken follicle stimulating hormone and chicken estradiol of laying hens, thereby effectively improving the stress level of the laying hens under the influence of the inspection robot without affecting the egg production rate, reducing the damage to the poultry body, increasing the yield and achieving better breeding efficiency. The method has the dual advantages of image acquisition quality optimization and animal welfare guarantee, and provides reliable technical support for intensive breeding.

The application provides application of an IGFBP1 gene in regulating chicken fat deposition traits, and belongs to the technical fields of biotechnology and genetic breeding. The application constructs a tissue-specific IGFBP1 recombinant adeno-associated virus vector, and uses the tissue-specific IGFBP1 recombinant adeno-associated virus vector to promote expression of the IGFBP1 gene of a chicken, promotes expression of the IGFBP1 gene of the chicken by promoting expression of the IGFBP1 gene of the chicken, increases the content of IGFBP1 protein in serum of the chicken, and further increases the content of intramuscular fat of the chicken, reduces the weight of abdominal fat of the chicken and the abdominal fat rate of the chicken. Through long-term experimental research, it is found that the chicken IGFBP1 can significantly affect the differential deposition of chicken IMF and AbF, the expression level of serum IGFBP1 protein is significantly negatively correlated with the weight of abdominal fat and the abdominal fat rate of the chicken, and is significantly positively correlated with the content of intramuscular fat of the chicken, and can be used as a basis for a serum judgment index of chicken fat deposition traits, and can also be used for screening chicken breeds with high intramuscular fat and low abdominal fat rate.

This invention discloses the application of FGA protein in improving egg production in chickens, belonging to the field of genetic engineering technology. Experiments have demonstrated that the FGA gene has a significant effect on improving egg production in chickens. Experimental results show that overexpression of the FGA gene can significantly increase the levels of reproductive hormones such as follicle-stimulating hormone, luteinizing hormone, estradiol, and progesterone in chicken serum, while significantly reducing prolactin concentration. Regarding follicle development, it can significantly increase the number of small yellow follicles, reduce the number of small white follicles, and promote follicle maturation. Regarding lipid metabolism regulation, it can significantly increase the concentrations of cholesterol, triglycerides, and very low-density lipoprotein in serum. This invention reveals for the first time the molecular mechanism by which the liver-specific endocrine factor FGA regulates egg production in chickens through interstitial communication, providing a new molecular target and application strategy for improving egg production in local chicken breeds.

The invention discloses a yellow feather broiler black abdomen character breeding method which comprises the following steps: step 1, measuring the brightness L * value of the abdomen color of a living hen to be measured, and measuring the testosterone concentration of serum of a living cock to be measured; 2, according to the measured L * value and the testosterone concentration of the chicken serum, removing living hens with the abdomen color brightness L * value smaller than 60 from the hens, and screening out hen breeding hens; in the cocks, the cocks with the testosterone concentration larger than 60 pg / ml in serum are removed, and breeding cocks are screened out. According to the breeding method for the black abdomen character of the chicken, a colorimeter is adopted for detecting the brightness L * value of the abdomen of the hen, breeding hens are screened, an ELISA kit is adopted for detecting the testosterone concentration value of serum, and breeding cocks are screened. And then the screened hens and cocks are used as breeding hens for large-scale breeding, the influence of black abdomen on the slaughtering appearance of the yellow feather broilers is reduced, the chicken skin color is uniform, and the market requirement is met. The breeding method disclosed by the invention can be widely applied to breeding of black abdomen characters of chickens, and has important significance on improving the breeding efficiency of chicken breeds.

A chicken serum-free medium for promoting proliferation of chicken primordial germ cells (PGCs) and a use of the chicken serum-free medium are provided. In the chicken serum-free medium, ovotransferrin is used instead of chicken serum. The ovotransferrin is used instead of chicken serum in a PGC culture system, and ovotransferrin can keep the self-renewing of PGCs to provide a basis for exploring a stable culture system. The chicken serum-free medium can avoid the adverse effects caused by an unclear composition of chicken serum. In addition, the accurate use of various growth factors can provide excellent culture conditions and reduce the culture cost.