6 results about "Chicken serum" patented technology

The application belongs to the technical field of biology and particularly relates to an ELISA kit for detecting chicken astrovirus type II antibodies and a preparation method. The Cell-ELISA experiment is carried out by using LMH cells infected with chicken astrovirus type II to detect the chicken astrovirus type II antibodies. The method has no cross reactivity with positive serum of Newcastle disease virus, avian influenza virus, chicken infectious anemia virus, avian leukosis virus, Marek's virus and chicken infectious bursal virus, which indicates that the method has good specificity. The Cell-ELISA method is used to detect the chicken serum sample to be detected by using whole virus infection, and is assembled into a kit for measuring chicken astrovirus antibodies in serum. The kit can be used for serological diagnosis of chicken astrovirus type II infection, monitoring of the level of antibodies, epidemiological investigation and the like, and provides a novel and effective detection means for prevention and treatment of chicken astrovirus type II.

The utility model relates to the technical field of production of chicken drops, in particular to a high-temperature and high-pressure sterilization device for chicken drops, which comprises a base, a sterilization tank and a hot water tank are mounted at the top of the base, and a drainage pipe for draining waste water is arranged at the bottom of the sterilization tank; the filtering mechanism comprises a filtering box which is fixedly connected to the end, away from the sterilization tank, of the drainage pipe, a filtering net is arranged in the filtering box, and an arc-shaped baffle is fixedly connected to the interior of the filtering box. The waste heat in the waste water is collected and utilized, the heated water body is supplemented into the hot water tank, heat waste is avoided, when the waste water in the sterilization tank is discharged, impurities in the waste water are filtered through the filter screen in the filter tank, residues of the impurities in the waste water are reduced, and follow-up waste water reutilization is facilitated.

Disclosed are a chicken serum-free culture medium for promoting the proliferation of chicken primordial germ cells and use thereof. Ovotransferrin is used in the culture medium instead of chicken serum. The effect of using ovotransferrin, instead of chicken serum, in a culture system for PGCs is disclosed. Ovotransferrin can maintain the self-renewal of PGCs, thereby providing a basis for developing a stable culture system. The culture medium can avoid adverse effects caused by the undefined components of chicken serum, and the precise use of various growth factors can provide better culture conditions and reduce culture costs.

The utility model relates to the technical field of drop chicken serum stock solution production, concretely relates to a kind of for drop chicken serum stock solution cooking device, it include: frame, the frame top is provided with top cover, the frame inside is provided with two symmetrical distribution's high-pressure cooking pot;Lifting mechanism, including fixedly connected in the drive motor of top cover outside, the drive motor is fixedly connected with threaded rod by output shaft, the threaded rod outside is connected with internal thread plate, the internal thread plate outside is equipped with winch, the utility model is taken out from high-pressure cooking pot when material, along with the contraction of rope, when net box is removed from high-pressure cooking pot, when round block enters into round pipe, under the location of round block and round pipe, net box is not easy to sway under the action of rope when moving, effectively prevent the spilling of drop chicken serum stock solution inside tray, avoid the waste of drop chicken serum stock solution when hoisting.

This invention discloses the application of FGA protein in improving egg production in chickens, belonging to the field of genetic engineering technology. Experiments have demonstrated that the FGA gene has a significant effect on improving egg production in chickens. Experimental results show that overexpression of the FGA gene can significantly increase the levels of reproductive hormones such as follicle-stimulating hormone, luteinizing hormone, estradiol, and progesterone in chicken serum, while significantly reducing prolactin concentration. Regarding follicle development, it can significantly increase the number of small yellow follicles, reduce the number of small white follicles, and promote follicle maturation. Regarding lipid metabolism regulation, it can significantly increase the concentrations of cholesterol, triglycerides, and very low-density lipoprotein in serum. This invention reveals for the first time the molecular mechanism by which the liver-specific endocrine factor FGA regulates egg production in chickens through interstitial communication, providing a new molecular target and application strategy for improving egg production in local chicken breeds.

A chicken serum-free medium for promoting proliferation of chicken primordial germ cells (PGCs) and a use of the chicken serum-free medium are provided. In the chicken serum-free medium, ovotransferrin is used instead of chicken serum. The ovotransferrin is used instead of chicken serum in a PGC culture system, and ovotransferrin can keep the self-renewing of PGCs to provide a basis for exploring a stable culture system. The chicken serum-free medium can avoid the adverse effects caused by an unclear composition of chicken serum. In addition, the accurate use of various growth factors can provide excellent culture conditions and reduce the culture cost.