65 results about "Gentamycin sulfate" patented technology

The external cultivating method of primordial bird germ cell relates to transgene, animal cloning, rare animal preservation, tissue and cell engineering and other science fields. After it is inoculated to the CEF raising layer, the primordial bird germ cell is cultivated in liquid high-sugar DMEM cultivation medium with added calf serum in 10 úÑ, chicken serum in 2 úÑ, L-glutamine in 2mmol / L, sodium pyrovate in 1 mmol / L, beta ¿Cmercaptoethanol in 5.5í‡10-5 mol / L, optional amino acid 10 microliter / ml, hSCF in 15-20 ng / ml, mLIF in 10 U / ml, bFGF in 10-15 ng / ml, hIL-11 in 0.04 ng / ml, IGF in 10 ng / ml, and gentamycin sulfate in 100 U / ml. The present invention has simple operation and high repeatability, and lays the foundation of the relevant research.

The invention belongs to the technical field of medicines and discloses a medicament for treating dental ulcer of infants. Gentamycin sulfate chewable tablets, prednisone acetate tablets, vitamin C tablets and vitamin B complex tablets (each tablet comprises main components of 13mg of vitamin B, 21.5mg of vitamin B, 60.2mg of vitamin B, 10mg of niacinamide and 1mg of dextro calcium pantothenate ) are used as raw materials, are mixed in the ratio according to different characteristics of each medicinal raw material, are dried at low temperature, ground, evenly blended and sterilized and subjected to other treatments and are prepared into western medicine powder or a spraying agent. The invention has the characteristics of new formula, simple manufacture and remarkable effect; and raw materials for preparing medicaments have wide source and low price, so the cost of medicinal products is reduced. Used by 120 infants suffered from infant dental ulcer, the total effective rate reaches 100 percent and special toxic and side effects are not found.

The invention aims to provide an improved method for extracting gentamycin sulfate from gentamicin fermentation broth. The method comprises the steps of fermentation broth filtration, adsorption through weakly acidic cation exchange resin, resolving, concentration, salt conversion and spray drying and the like. Gentamicin is one of main antibacterial drugs used for treating various gram-negative bacteria infections clinically at present and is a multicomponent mixture composed of 2-deoxystreptamine, magenta glycosylamine and garosamine and the like. According to the extraction method, the adsorption is performed through the cation exchange resin, resolving is performed through sodium hydroxide solution, decoloration is performed through anion exchange resin, nanofiltration concentration desalting is performed, finally salt conversion is performed, active carbon decoloration is performed, and the finished product is obtained through spray drying. The method has the advantages of being high in production efficiency, less in environmental pollution and good in finished product quality.

The invention discloses a compound gentamycin sulfate soluble powder and a preparation method thereof, aiming at providing the compound gentamycin sulfate soluble powder and the preparation method thereof, which is used for treating pig bacterial enteritis and has the advantages of fast effect, safe use, small side effect and drug resistance ratio reduction of pathogenic bacteria. The compound gentamycin sulfate soluble powder comprises the following components in weight percent, 3% to 15% of gentamycin sulfate, 5% to 15% of maquindox, 3% to 15% of colistin sulphate, 2% of vitamin K3, and thebalance water. The invention adopts three antimicrobial components of the gentamycin sulfate, the maquindox and the colistin sulphate; the gentamycin sulfate and the colistin sulphate are difficult toabsorb when being eaten, and can kill the pathogenic bacteria through contact so as not to strengthen burden to pig bodies caused by drug; the maquindox is easy to absorb when being eaten, and is widely distributed in the bodies so as to effectively kill bacteria in the blood. The gentamycin sulfate, the maquindox and the colistin sulphate are combined for use so as to effectively kill the pathogenic bacteria, and greatly reduce the drug resistance ratio of the pathogenic bacteria causing the pig bacterial enteritis; and the vitamin K3 has the functions of stopping and restraining blooding.

The invention belongs to the technical field of veterinary medicine, specifically to an injection for preventing and treating porcine viral diseases and a preparation method thereof. Every 1000ml of the injection contains 1 to 100g of thymopoietin, 1 to 10*10 <8> IU of interferon, 10 to 50 million units of gentamycin sulfate, 0.1 to 2g of antioxidant, 0.2 to 0.4g of protective agent, 50 to 100ml of buffer, 20 to 80ml of osmotic pressure adjusting agent, a pH adjusting agent and the balance of water for injection. The preparation in the invention is wide in application, fast in curative effect, little in medicine dosage, low in cost, and difficult to bring germ contamination. The injection reduces the stress reaction caused by capturing the animal for many times in injecting, and overcomes the shortcoming that the water needle made of thymopoietin and interferon is unstable.

The present invention discloses a piglet yellow and white scour treatment compound traditional Chinese medicine composition and a preparation method thereof. The preparation method specifically comprises: carrying out decoction, filtration, concentration and spray drying on Chinese pulsatilla root, tomentose populus ament, coptis chinensis, amur corktree bark and medicine terminalia fruit, then adding western medicine such as mequindox, gentamycin sulfate and sodium salicylate and auxiliary materials such as sucrose and Jiayi powder, and carrying out mixing, crushing, soft material preparation and drying. The piglet yellow and white scour treatment compound traditional Chinese medicine composition has the following characteristics that: significant treatment effects are provided; water drinking and material stirring are adopted to direct take the composition; the composition is subjected to complete metabolism absorption in the body; and the composition has characteristics of good treatment efficacy, easy use, no toxic-side effect, safety and reliability, and is suitable for piglet dysentery treatment, especially for piglet yellow and white scour treatment.

The invention discloses long-term preservation liquid for corneal tissue and a preparation method thereof. The long-term preservation liquid is prepared from the following components: 15 to 25g / L of sodium chondroitin sulfate, 0.5 to 4g / L of sodium hyaluronate, 0.5 to 3 g / L of dextran 40, 15 to 30 g / L of glycerin, 0.001 to 0.3 g / L of sodium pyruvate, 0.002 to 2 g / L of vitamin C, 0.05 to 0.7 g / L ofgentamicin sulfate, 1.4 to 2.2 g / L of sodium bicarbonate, 5.0 to 6.5 g / L of 4-hydroxyethylpiperazine ethanesulfonic acid, and 1 L of water for injection. A combination system of sodium chondroitin sulfate, sodium hyaluronate and dextran is adopted to obtain the preservation liquid which can be used for long-term preservation of corneal tissue, thereby being particularly advantageous for maintaining an original collagen fiber structure and transparency of a corneal lens for a long term; after the cornea tissue is preserved by the long-term preservation liquid, the cornea tissue can be used directly after rewarming, the operation is simple, and rehydration is not needed.

The invention relates to a medicinal preparation for curing calf diarrhea and applications thereof, and belongs to the technology field of veterinary medicinal preparation. In order to solve the problems of high cost, bad curative effect and slow effect in the prior art, the invention provides a medicinal preparation for curing calf diarrhea. The compound medicinal preparation is mainly composed of following drugs in parts by weight: 6.0 to 12 parts of gentamycin sulfate (GEN), 15 to 30 parts of rhizoma corydalis, 15 to 20 parts of yeast powder, 15 to 30 parts of alum, 15 to 25 parts of sodium fulvate, 15 to 25 parts of clove, 15 to 30 parts of white pepper, 15 to 30 parts of evodia and 15 to 25 parts of Chinese cassia tree. The compound medicinal preparation has the advantages of low cost, high efficiency on curing calf diarrhea and high cure rate. Furthermore the components are used together to have a synergistic effect, and has the functions of invigorating stomach, regulating Qi, disinfecting, diminishing inflammation, astringing to arrest diarrhea, relieving pains and stopping bleeding.

The invention provides gentamycin sulfate tablets and a preparation method thereof. The stability of the gentamycin sulfate tablets is effectively increased by altering the auxiliary formula of the original production process and the quality problem that the gentamycin sulfate tablets are easy to crack during storage is solved. The auxiliary formula of production technology comprises the following components by weight percent: 40-70% of starch, 1-10% of microcrystalline cellulose, 10-30% of calcium sulfate and 1-10% of PEG6000. In the product, the water content of particles is reduced and controlled to 1.0-6.0%, and the drying temperature of the original process is reduced and controlled to 55-80 DEG C. The prepared gentamycin sulfate tablets of the invention have smooth surface of tablets and stable performance, the product is applicable to mass production, and the product quality meets the requirements of the China Pharmacopeia.

The invention discloses a preparation method of nitrogen a gentamycin sulfate injection. The method comprises the following steps: installing a nitrogen distributor in a dispensing tank; taking water for injection accounting for 50-60% of the total volume, placing gentamycin sulfate raw powder in the dispensing tank to stir and dissolve, adding accessories and a defined amount of water for injection; adjusting the pH value of the dispensed solution; and after the test proving that the medicine content is qualified, decarbonizing, performing aseptic filter-pressing, and filling. The accelerating test and sample observation show that the gentamycin sulfate injection prepared by the preparation method of the invention can not change color and the color of the same lot is uniform. The color grade qualification rate of the product can reach 100% under the specified storage conditions in the expiration date of the product.

The invention relates to the field of pearl mussel cultivation, in particular to a core insertion operation system disinfection method for the pearl mussels with cores. The method of the invention mainly solves the key technical problems during the core insertion operation process in the prior art such as disinfection steps, selecting medicines, formulas, etc. The invention provides an effective method for the system disinfection during the core insertion operation process of the pearl mussels with cores and leads the survival rate of the operated mussels to be improved to more than 90%. The technical proposal of the invention is that disinfector compounded by trichloride isocyanuric acid and potassium permanganate is used for carrying out the environmental disinfection; the disinfector compounded by norfloxacin and compound matrine injection is used for carrying out the absorbent cotton disinfection; the disinfector compounded by gentamycin sulfate injection and compound amino acid injection is used for carrying out the disinfection of core sheets and cuts; disinfection solution compounded by clam powder and norfloxacin is used for carrying out the pearl mussel disinfection after the operation.

The invention relates to a preparation method of granules for diminishing inflammation, relieving pain and promoting gastric mucosa repair. The preparation method comprises the following steps of: crushing procaine hydrochloride and cane sugar till the particle sizes of the two components can be 80 meshes; dosing and weighing, wherein gelatin is taken, and the weight ratio of gentamycin sulfate (calculated by anhydride) to procaine hydrochloride (calculated by anhydride) to vitamin B12 to the cane sugar is 20mg: 0.1g: 0.02mg: 4.81g; preparing a binding agent; granulating, wherein the procaine hydrochloride and the cane sugar are sequentially poured into a fluidized bed drying granulator, solution of the gentamycin sulfate and the vitamin B12 is sprayed into the mixture after the procaine hydrochloride and the cane sugar are fully mixed for 15 minutes, then aqueous solution of the gelatin is taken as the binding agent for spraying fluidized granulation, the granulation temperature is controlled in a range of 50+ / -5 DEG C, the drying temperature is controlled in a range of 65+ / -5 DEG C, the drying lasts for 20 minutes and stops after the granules are formed and then the granules are obtained; granulating; and totally mixing the granules. The preparation method has the advantage of obtaining the granules with good quality.

The invention discloses a medicinal composition, which comprises pharmaceutically effective dose of tolazoline hydrochloride, gentamycin sulfate, ribavirin, dexamethasone sodium phosphate, chlorphenamine maleate and a pharmaceutical carrier. The medicinal composition can be used for treating various acute or chronic rhinitis, sinusitis, purulent rhinitis, rhinitis sicca, atrophic rhinitis, allergic rhinitis and the like.

The invention discloses an injection for treating piglet bacillary dysentery and a preparation method thereof. 1000ml of injection comprises the following raw materials: 6-15g of Chinese anemone extractive, 5-10g of ash bark extractive, 5-15g of radix scutellariae extractive, 5-10g of amur corktree bark extractive and 10-20g of gentamycin sulfate. The invention mainly treats the piglet bacillary dysentery, has obvious curative effect particularly on piglet yellow-white dysentery, adopts an injection mode for drug administration, solves the difficulties of low drug bioavailability caused by incomplete piglet bioavailability function, and has the advantages of fast response, good curative effect, convenient use, no toxic or side effect, safety and reliability.

The invention relates to a veterinary compound gentamycin sulfate injection and a preparation method thereof. The injection consists of gentamicin sulphate, ofloxacin, analgin, trimethoprim, an organic solvent and injection water. The injection serving as a veterinary special compound preparation is mainly used for treating porcine epidemic diarrhea, transmissible gastrocnteritis, paratyphus vaccine for piglets, yellow and white scour for piglets, edema disease for piglets, and intestinal diseases, such as poultry colibacillosis, fowl typhoid, paratyphoid and the like. The injection has the advantages of convenient use, short period of treatment, low medicament resistance and the like.

The invention relates to a novel process for decarburizing gentamycin sulfate. In the process, a gentamycin sulfate solution subjected to salt conversion can be directly sprayed for drying after being statically adsorbed and decolored by an active carbon fixed bed to obtain a finished product of gentamycin sulfate. The used active carbon can be recycled after being reproduced. The process thoroughly overcomes the defects in the traditional production of the gentamycin sulfate of complicated process of heavy extraction filling press equipment, high labor intensity for operation, lower standardbatch, multiple decarburization operations and the defects that the carbon cannot be thoroughly cleaned and the decolored active carbon cannot be recycled. Meanwhile, the unit of concentrated solution and the consumption of steam can be reduced and the production cost can be effectively reduced; and the novel process has remarkable energy-saving effect.

The invention discloses a novel nano gold composite antibacterial agent and a preparation method thereof. The novel nano gold composite antibacterial agent is gentamycin sulfate loaded nano gold particles. The preparation method comprises following steps: adding polyvinylpyrrolidone into chloroauric acid, evenly stirring, adding gentamycin sulfate, then adding a sodium borohydride solution to reduce chloroauric acid and load gentamycin sulfate, and removing unloaded gentamycin sulfate in the solution through dialysis to obtain the nano gold composite antibacterial agent. The nano gold composite antibacterial agent is prepared by a one-step reduction method, the technical process is simple, the method is green and environmentally friendly, moreover, the size of the prepared nano gold composite antibacterial agent is controllable, the particle size distribution is narrow, the average particle size is 4.7 nm, the nano particles are small, and agglomeration is difficult to happen. Throughan electrostatic adsorption effect, gentamycin sulfate is loaded on nano gold, and the antibacterial agent has a broad-spectrum antibacterial property and a synergetic antibacterial property and can well inhibit Gram-positive bacteria, Gram-negative bacteria, and fungi.

The invention discloses a composition for treating fowl cholera and a preparation method thereof. The invention aims to provide a composition which treats the fowl cholera by treating both manifestation and root cause of disease, reduces the probability of medicine resistance in pathogenic bacteria, and strengthens therapeutic effect and a preparation method thereof. The composition consists of the following raw materials in percentage by weight: 3 to 10 percent of norfloxacin nicotinic, 3 to 10 percent of gentamycin sulfate, 5 to 15 percent of taurine, 5 percent of sodium chloride, 5 percent of potassium chloride, and the balance of anhydrous glucose. In the composition, the combination of the norfloxacin nicotinic and the gentamycin sulfate is adopted to produce a double synergistic bacteria-resistance effect, effectively reduce the probability of medicine resistance of pasteurella, and improve sensitivity and antibacterial effect. The taurine has the effects of reducing fever and resisting inflammation; the sodium chloride and the potassium chloride supplement electrolyte; and the combination of the medicines according to a reasonable proportion treats the fowl cholera by treating both manifestation and root cause of disease, reduces the probability of medicine resistance of the pathogenic bacteria, strengthens therapeutic effect, is convenient for use, and reduces the phenomenon of unsuccessful medication caused by diagnosis reasons.

The invention discloses a chemiluminescent substrate solution and a procalcitonin detection kit utilizing the same. The chemiluminescent substrate solution is prepared from 0.8-1.5 g / L of luminol or its derivatives, 0.05-0.6 g / L2 of amino-4-nitro phenol, 0.01-0.05 mg / L of 9,9-dihexyl-2,7-dibromofluorene, 3-6 g / L of polyvinyl alcohol, 6-20 g / L of polyvinylpyrrolidone, 3-4.5 g / L of polyethylene glycol 600, 100-300 million units / L of gentamycin sulfate, 0.1-1.0 g / L of carbamide peroxide and 0.1 mol / L of a Tris-HCl buffer solution with the pH of 9.0. The chemiluminescent substrate solution is a single solution, can not only achieve the HRP enzyme catalyzed chemiluminescence reaction and can also effectively improve the luminous intensity and decrease a luminous background to achieve high-sensitivity detection.

An orally-taken infusion of gentamycin sulfate is prepared through mixing cyclodextrin with gentamycin sulfate, mixing with xylitol or pectose, granulating, and coating the particles by sodium thiosulfate (or sulfite).

A compound medicine in the form of tablet for treating gastritis contains proportionally gentamycin sulfate, procaine hydrochloride, VB12 and medicinal additives.

The invention discloses gentamycin sulfate injection containing a buffer agent. Each milliliter of injection consists of 18 to 45 milligrams of gentamycin sulfate, an antioxidant, a pH buffer agent and the balance of injection water, wherein the antioxidant is one of sodium sulfite, sodium bisulfite or sodium pyrosulfite. The injection is characterized in that the pH value of the pH buffer agent is 5.5 to 6.0.

The invention provides an extract of rust-coloured crotalaria herb with root for treating epicophosis and tinnitus, and a preparation method and application thereof. Pharmacological tests prove that the extract of the rust-coloured crotalaria herb with root for treating epicophosis and tinnitus can inhibit hearing loss further caused by gentamycin sulfate, and can prevent an animal hearing loss effect caused after injection of gentamycin; and during clinical application, the effective rate of mild epicophosis is 10 percent, and the effective rate of moderate and heavy epicophosis is greater than 50 percent.

The invention provides an enzyme conjugate stabilizing solution which comprises the following components: 1-7.5g / L protein material, 5-50g / L saccharide material, 2-30g / L amino acid material, 5-50g / L antipyrine, 1-10 g / L vitamin C, 0.2-5g / L surfactant, 0.5-5 million IU / L gentamycin sulfate, 0.5g / L Proclin 300 and 0.02mol / L phosphate buffer solution the pH value of which is 7.4, wherein the protein material is bovine serum albumin or casein; the saccharide material is trehalose, gluecose, lactose or cane sugar; the amino acid material is glycine, phenylalanine, praline, tyrosine, serine, cysteine, methionine, glutamoyl or lysine; the surfactant is Twain-20, Twain-80 or triton x-100. The enzyme conjugate stabilizing solution provided by the invention solves the problem that the enzyme conjugate is easy to lose activity after the enzyme is not in the low temperature storage environment in related technology.

The invention discloses a compound common andrographis herb injection for treating high fever of pigs and a preparation method thereof. The invention aims to provide the compound common andrographis herb injection and the preparation method thereof, wherein the compound common andrographis herb injection can treat the high fever caused by pig bacteria and virus composite infection, has quick treatment response, can treat both principal and secondary aspects of diseases, reduces taking frequency and is convenient to take; and the preparation method has a simple process and is easy to implement. Each 100L of injection comprises 3 to 7 kg of common andrographis herb extractum, 0.5 to 1.5kg of zedoary oil, 1 to 6kg of gentamycin sulfate, 1.5 to 4.5kg of ofloxacin, 8 to 30kg of analgin, 0.05kg of ammonium chloride bethanechol, 0.2kg of anhydrous sodium bisulfite, 0.01kg of EDTA-2Na, 1kg of Tween-80, 0.5kg of hydrochloric acid, and the balance of water for injection. The injection has the effect in many aspects at the same time by the reasonable proportion of a plurality of medicaments, has quick response to treat the high fever caused by the pig bacteria and the virus composite infection, is directly applied without diagnosis and can achieve the treatment effect of a plurality of medicaments when used at one time.

The related method for gentamycin sulfate detection standard curve comprises: coupling protein and gentamycin sulfate to inject into rat body and prepare antibody, selecting the work concentration for antigen and antibody reaction by chess board method, and selecting holes with OD450 value as 1-1.5 to calculate and draw the curve. The enzyme linked immunity fast detection method comprises: 1. diluting antibody, adding gradient-diluting sample, enzyme link reacting for 1-1.5h at 41Deg, developing with TMB; 2. selecting the sample with 0.4-1.5OD value; 3. checking data in curve, and multiplying dilution time to obtain the residual gentamycin sulfate. This detection method needs time only 1-1.5h.

The invention discloses an immune cell culture solution which is prepared by adding autologous plasma, human serum albumin, gentamycin sulfate, immune cell growth factor interleukin-2, sodium bicarbonate, phytic acid and vitamin C into a basic complete solution. The immune cell culture solution has the advantages of overcoming the defects that lymphocyte culture solution available in the existing market is unstable, number of immune cells cultured by the lymphocyte culture solution is small and purity of the immune cells is low, and is capable of culturing sufficient amount of high-activity immune cells.

The invention relates to an ointment containing nano-scale gentamycin sulfate and cefaclor for treating duck leg diseases and a preparation method thereof. The main medicaments of the ointment preparation are nano-scale powder, the ointment preparation can be applied to skin directly, and has the advantages of strengthening the target effect of medicaments, convenient administration, quick absorption, direct achievement to deeper skin, strong sterilization, small side effect and the like. The ointment preparation can effectively treat the duck leg diseases due to duck leg infection caused by staphylococcus, pseudomonas aeruginosa and other bacteria, so that the ointment containing the nano-scale gentamycin sulfate and the cefaclor can sufficiently play the effect of the medicament when treating the duck leg diseases.

The invention discloses a composition for treating infection of pseudomonas aeruginosa in chicken and a preparation method thereof, aiming to provide a composition for treating the infection of pseudomonas aeruginosa in chicken, which is capable of reducing the drug resistance rate of bacteria and addressing both the symptoms and root causes, and a preparation method thereof. The composition consists of the following components in percentage by weight: 4 to 12 percent of gentamycin sulfate, 4 to 8 percent of amoxicillin, 4 to 8 percent of sodium carbonate, 10 to 30 percent of analginum, and the balance of anhydrous dextrose. In the composition, the gentamycin sulfate and the amoxicillin are synergized to kill pseudomonas aeruginosa, can reduce the occurrence probability of pathogeny drug resistance, and increase the sensitivity of pathogeny to the composition; the sodium carbonate has the effect of solubilizing the amoxicillin, and can improve the antibacterial effect of the gentamycin sulfate; and the analginum has the effect of defervescence and anti-inflammatory. The composition has the functions of killing the pathogency, effectively resisting pathogenic bacteria and clinical symptoms caused by the pathogenic bacteria, addressing both the symptoms and root causes, effectively treating the infection of pseudomonas aeruginosa in chicken, and reducing the pathogeny drug resistance by reasonable compatibility of the components. The composition can be dissolved in water, and is convenient to use.

The invention relates to a medicament for treating chronic enteritis and a preparation method thereof. The medicament is prepared from the following raw materials in part by weight: 75 to 105 parts of Chinese thorowax root, 38 to 53 parts of baical skullcap root, 75 to 105 parts of nutgrass galingale rhizome processed with vinegar, 75 to 105 parts of costus root, 75 to 105 parts of szechuan lovage rhizome, 75 to 105 parts of oriental waterplantain rhizome, 21 to 33 parts of berberine hydrochloride, 15 to 21 parts of metronidazole, 45 to 63 parts of gentamycin sulfate and 21 to 33 parts of lactasin. The medicament is prepared by the following steps of: stewing, frying to yellowish, preparing into powder, uniformly blending, and preparing into capsules. The medicament is easy to prepare, isconvenient to take, and has a quick curative effect and a good effect. According to statistics, among 35 patients who take the medicament, 32 patients are cured, the medicament has an obvious effect on 1 patient and has an effect on 1 patient, the total effective rate is 97.143 percent, and users do not have adverse effects.