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Immune cell culture solution

A technology of immune cells and culture medium, which is applied in the field of culture medium for immune cell culture, and can solve the problems of insufficient culture quantity and low purity

Inactive Publication Date: 2017-08-18
湖南惠益森细胞基因工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the problems of insufficient quantity and low purity of conventional culture medium in the above-mentioned in vitro culture, and provide a culture medium for immune cell culture and its application.

Method used

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Embodiment Construction

[0012] Specific implementation plan

[0013] In order to make the objectives, technical solutions and advantages of the present invention clearer, the following further describes the present invention in detail without limiting the present invention.

[0014] The present invention provides a culture medium for culturing immune cells, which includes the following steps:

[0015] 1. The configuration of the culture medium:

[0016] 1000ml basic culture medium contains the following ingredients:

[0017]

[0018] 2. Mononuclear cells isolated from human peripheral blood in vitro:

[0019] 1) Collect 50ml of peripheral blood from volunteers with heparin sodium as the anticoagulant. Transfer the collected blood to a new centrifuge tube in a biological safety cabinet and centrifuge at 500g for 10 minutes. After centrifugation, the upper layer is prepared with autologous plasma and the lower layer is separated and prepared. Monocytes.

[0020] 2) Mix human peripheral blood cells and physiologic...

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Abstract

The invention discloses an immune cell culture solution which is prepared by adding autologous plasma, human serum albumin, gentamycin sulfate, immune cell growth factor interleukin-2, sodium bicarbonate, phytic acid and vitamin C into a basic complete solution. The immune cell culture solution has the advantages of overcoming the defects that lymphocyte culture solution available in the existing market is unstable, number of immune cells cultured by the lymphocyte culture solution is small and purity of the immune cells is low, and is capable of culturing sufficient amount of high-activity immune cells.

Description

Technical field [0001] The invention relates to a culture medium for culturing immune cells. Background technique [0002] Immune cells refer to cells involved in or related to immune responses, including lymphocytes, dendritic cells, and monocytes. Immune cells and immune organs form the immune system, which protects the body from harm. Human immunity reaches its peak at the age of 20, only 1 / 2 of its peak at 40, and only 1 / 10 of its peak at 70. Decreased immunity means that the ability of immune cells to fight viruses, bacteria and cancer has decreased year by year, and the risk of disease has also increased. [0003] Abnormal immune cells may cause various serious diseases, such as systemic lupus erythematosus, because there are a large number of pathogenic autoantibodies and immune complexes in the body that cause tissue damage. Cancer occurs because tumor cells escape the supervision of immune cells and grow uncontrollably. Therefore, storing healthy immune cells for re-ex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0783
CPCC12N5/0638C12N2500/30C12N2500/38C12N2500/84C12N2501/2301C12N2501/2302C12N2501/24C12N2501/998
Inventor 李志远王利群邓思浩汤沙严波杨雅评郭海江
Owner 湖南惠益森细胞基因工程有限公司
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