8 results about "Cell engineering" patented technology

The present application belongs to the field of molecular biology and cell engineering technology, and particularly relates to application of a protein encoded by a trim25 gene in improving fish cell transfection efficiency. The present application aims to solve the problem of low fish cell transfection efficiency. The technical problem of the present application is the application of the protein encoded by the trim25 gene in improving fish cell transfection efficiency; the amino acid sequence of the protein is shown as SEQ ID No. 1. The present application first explicitly defines the role of the protein encoded by the trim25 gene in fish cell transfection efficiency. The method of the present application is simple to operate, low in cost and good in repeatability, and provides an efficient tool for fish gene function research, gene editing and the like.

The invention discloses an immortalized porcine fibroblast fat progenitor cell line as well as a construction method and application thereof, and belongs to the technical field of stem cell biology and cell engineering. Pig muscle is taken as a source, primary porcine fibroblast fat progenitor cells are obtained through separation and screening, pTERT and pCDK4 genes are overexpressed at the same time through a genetic engineering method, the cell line can break through the Harike limit, immortalization is achieved, the cell line is transferred to 43 generations at present, the cell is in a healthy and full long shuttle-shaped state, and the progenitor cells can be used as progenitor cells of the porcine fibroblast fat progenitor cells of the porcine fibroblast fat progenitor cells of the porcine fibroblast fat progenitor cells. Long-term in-vitro culture still has adipogenic differentiation capacity and can be used for preparing products such as cell culture meat.

The invention relates to a multi-monomer engineering intelligent time sequence optimization method, which comprises the following steps of: according to a construction scheme, numbering components, and obtaining space information and time information of the components; inputting the number, the spatial information and the time information into a BIM model to generate a space-time matrix; calculating space-time cross points according to the space-time matrix to obtain potential conflict points; key blocking points are found in the potential conflict points according to the construction logic relation; optimizing the construction time sequence based on a reinforcement learning algorithm, and outputting an optimization scheme; executing the optimization scheme, and dynamically updating data according to the construction progress; and repeating the steps to optimize the construction time sequence until the construction is completed. Through space-time matrix calculation, hidden conflicts are found in advance, a time sequence optimization scheme is generated based on a reinforcement learning algorithm, construction efficiency is improved, construction progress data are updated and re-optimized in real time, onsite emergencies can be dealt with, and adaptability is high.

The invention belongs to the technical field of molecular biology and cell engineering, and particularly relates to application of protein coded by a trim25 gene to improvement of fish cell transfection efficiency. The technical problem to be solved by the invention is low fish cell transfection efficiency. The invention aims to solve the technical problem of application of protein coded by a trim25 gene in improvement of fish cell transfection efficiency. The amino acid sequence of the protein is as shown in SEQ ID No. 1. According to the invention, the effect of the protein coded by the trim25 gene in the fish cell transfection efficiency is defined for the first time. The method is easy and convenient to operate, low in cost and good in repeatability, and an efficient tool is provided for fish gene function research, gene editing and the like.

This invention discloses the application of FGA binding to Caspase3 in regulating ovarian granulosa cells, belonging to the fields of cell engineering and genetic engineering. Using FGA as the research object, this invention employs molecular and cell biology methods to study its application in ovarian granulosa cells, verifying from multiple levels and perspectives that the FGA gene regulates the growth and development of ovarian follicles and affects the onset of puberty in mice. This invention can provide new reference for the genetic improvement of sow reproductive performance and has good application value.

The present disclosure provides systems, methods, and apparatuses for improved magnetic separation of a biological population. A system for magnetic separation and collection of a target biological population from a biological sample comprises a cell engineering cassette, and a fluidic pathway disposed within the cell engineering cassette. The fluidic pathway has entrapment features disposed along a flow path of the fluidic pathway. An array of magnets is disposed adjacent to the fluidic pathway and the cell engineering cassette such that the array of magnets can be translatable toward and away from the fluidic pathway to apply a magnetic field to the biological population for the separation of a first subpopulation from a second subpopulation.

The present disclosure provides cassettes for use in automated cell engineering systems that include cell concentration filters for reducing fluid volume of a cell sample during or following automated processing. The disclosure also provides methods of concentrating a cell population, as well as automated cell engineering systems that can utilize the cassettes and carry out the methods.

The present disclosure provides systems, methods, and apparatuses for improved magnetic separation of a biological population. A system for magnetic separation and collection of a target biological population from a biological sample comprises a cell engineering cassette, and a fluidic pathway disposed within the cell engineering cassette. The fluidic pathway has entrapment features disposed along a flow path of the fluidic pathway. An array of magnets is disposed adjacent to the fluidic pathway and the cell engineering cassette such that the array of magnets can be translatable toward and away from the fluidic pathway to apply a magnetic field to the biological population for the separation of a first subpopulation from a second subpopulation.