6 results about "L-glutamine" patented technology

This invention relates to microorganisms that produce L-glutamic acid and a method for producing amino acids. The method involves culturing microorganisms in a culture medium that have the ability to produce one or more amino acids selected from L-glutamic acid, L-glutamine, L-proline, L-ornithine, L-citrulline, and L-arginine, and that have been modified to increase the activity of α-ketoglutarate synthase, thereby generating and accumulating the L-amino acids in the culture medium or in the cells, and collecting the L-amino acids from the culture medium or in the cells.

The invention belongs to the technical field of probiotic protection, and discloses a preparation method and application of probiotic-loaded sulfydryl hyaluronic acid-based hydrogel, sulfydryl hyaluronic acid is prepared by taking cysteamine hydrochloride as a substrate, and connection of L-glutamine is further catalyzed through a cross-linking agent, so that the sulfydryl hyaluronic acid-based hydrogel is obtained. The cross-linking agent is a mixture of N-hydroxysuccinimide and 1-ethyl-(3-dimethylaminopropyl) carbodiimide or a mixture of N-hydroxysuccinimide and 1-hydroxybenzotriazole (HOBT), and the cross-linking agent is a mixture of N-hydroxysuccinimide and 1-ethyl-(3-dimethylaminopropyl) carbodiimide and 1-hydroxybenzotriazole (HOBT). The hydrogel prepared by the preparation method disclosed by the invention is used as a probiotic carrier, so that the influence of complex gastrointestinal tracts is effectively resisted, the hydrogel accurately reaches an intestinal injury part and is split, and the targeted intestinal controllable release of probiotics is realized; meanwhile, the L-glutamine is introduced and has a synergistic effect with the probiotics to relieve specific intestinal diseases, so that the L-glutamine can be used as a probiotic supplement for the specific intestinal diseases.

This invention discloses a culture medium and process for alleviating ammonium accumulation in cells. The concentrations of the substances added to the culture medium are as follows: 2 g / L glutamate, 0 g / L glutamine, 2 g / L aspartic acid, 2 g / L asparagine, 1 g / L serine, 1 g / L pyruvate, 0.5 g / L α-ketoglutarate, 0.5 g / L oxaloacetic acid, 0.5 g / L proline, 0 g / L glucose, 8 g / L galactose, and 0.1% glycerol. Compared with the prior art, the advantages of this application are: this method mainly achieves the purpose of maintaining cell viability and reducing the cell metabolic byproduct ammonium ions by adjusting the proportion of substances related to cell metabolic pathways and adding additional cell protectants; using the optimized culture medium of this invention, the cell viability can be increased from 42.38% to 95.21% and the ammonium ion concentration can be reduced from 6.308 mM to 0.914 mM by day 7.

The invention provides an animal microecological preparation with an anti-stress function and a preparation method thereof, and belongs to the technical field of microecological preparations, and the preparation method comprises the following steps: culturing bacillus subtilis and lactic acid bacteria to obtain a bacillus seed solution and a lactic acid bacteria seed solution, and carrying out aerobic fermentation on the bacillus seed solution to obtain a fermentation liquid; adding a lactic acid bacteria seed solution for micro-aerobic fermentation; culturing saccharomyces cerevisiae to obtain a saccharomyces cerevisiae seed solution, mixing the fermentation product with the saccharomyces cerevisiae seed solution, and sequentially centrifuging and freeze-drying to obtain freeze-dried bacterial powder; the preparation method comprises the following steps: mixing freeze-dried bacterial powder, prebiotics, chlorogenic acid, L-glutamine, yeast beta-glucan and deionized water to obtain a core material solution, mixing the core material solution with a sodium alginate solution, dropwise adding into a calcium chloride solution for crosslinking, soaking in a chitosan solution, soaking in a sodium tripolyphosphate solution again for crosslinking, and carrying out spray drying. Through synergistic fermentation of multiple strains and composite embedding of functional components, the anti-stress function of the animal microecological preparation is improved.

The invention discloses an adipogenesis induction culture medium suitable for natural immortal cells of chicken fat precursor cells, an adipogenesis induction method and application of the adipogenesis induction culture medium and the adipogenesis induction method, and belongs to the technical field of animal cell differentiation. The fat formation induction culture medium comprises a basic culture medium, fetal calf serum with the volume percentage being 0.1-2%, penicillin with the concentration being 100 U / mL, streptomycin with the concentration being 100 [mu] g / mL, OA with the concentration being 60-150 [mu] mol / L, EPA with the concentration being 40-80 [mu] mol / L, DHA with the concentration being 20-100 [mu] mol / L, insulin with the concentration being 5-10 [mu] g / mL, transferrin with the concentration being 10-20 [mu] g / mL and glutamine with the concentration being 2-4 mmol / L. The adipogenic differentiation effect of the adipogenic induction culture medium is good, the cell differentiation efficiency reaches 100%, the fat maturity is obviously improved, and the repeatability is good.

The invention discloses a culture medium composition for inducing pluripotent stem cells to be differentiated into insulin-secreting cells and application of the culture medium composition, and belongs to the technical field of cell differentiation. The culture medium combination comprises an induction culture medium 1 and an induction culture medium 2, wherein the induction culture medium 1 comprises 80-90 vol% of a DMEM high glucose culture medium, 10-20 vol% of FBS, 1.5-2.5 mmol / L of glutamine, 1.5-2.5 mmol / L of non-essential amino acid, 8-12 mmol / L of nicotinamide, 8-12 [mu] g / L of activin A, 8-12 [mu] g / L of hepatocyte growth factors and 8-12 [mu] g / L of beta-cytokine, and the pH value of the induction culture medium 1 is 7.2-7.4; the induction culture medium 2 comprises 94 to 98 vol% of a DMEM / F-12 culture medium, 2 to 6 vol% of FBS, 1.5 to 2.5 mmol / L of glutamine, 1.5 to 2.5 mmol / L of non-essential amino acid, 8 to 12 mmol / L of nicotinamide, 20 to 30 [mu] g / mL of insulin, 0.8 to 1.2 [mu] g / mL of laminin and 8 to 12 [mu] g / L of activin A, and the pH value of the induction culture medium 2 is 7.2 to 7.4. The method for inducing the pluripotent stem cells to be differentiated into the insulin-secreting cells by using the culture medium combination is short in time consumption, only needs 8 days, and is simple in process and lower in cost.