3 results about "L-glutamine" patented technology

This invention relates to microorganisms that produce L-glutamic acid and a method for producing amino acids. The method involves culturing microorganisms in a culture medium that have the ability to produce one or more amino acids selected from L-glutamic acid, L-glutamine, L-proline, L-ornithine, L-citrulline, and L-arginine, and that have been modified to increase the activity of α-ketoglutarate synthase, thereby generating and accumulating the L-amino acids in the culture medium or in the cells, and collecting the L-amino acids from the culture medium or in the cells.

This invention discloses a culture medium and process for alleviating ammonium accumulation in cells. The concentrations of the substances added to the culture medium are as follows: 2 g / L glutamate, 0 g / L glutamine, 2 g / L aspartic acid, 2 g / L asparagine, 1 g / L serine, 1 g / L pyruvate, 0.5 g / L α-ketoglutarate, 0.5 g / L oxaloacetic acid, 0.5 g / L proline, 0 g / L glucose, 8 g / L galactose, and 0.1% glycerol. Compared with the prior art, the advantages of this application are: this method mainly achieves the purpose of maintaining cell viability and reducing the cell metabolic byproduct ammonium ions by adjusting the proportion of substances related to cell metabolic pathways and adding additional cell protectants; using the optimized culture medium of this invention, the cell viability can be increased from 42.38% to 95.21% and the ammonium ion concentration can be reduced from 6.308 mM to 0.914 mM by day 7.

The invention discloses an adipogenesis induction culture medium suitable for natural immortal cells of chicken fat precursor cells, an adipogenesis induction method and application of the adipogenesis induction culture medium and the adipogenesis induction method, and belongs to the technical field of animal cell differentiation. The fat formation induction culture medium comprises a basic culture medium, fetal calf serum with the volume percentage being 0.1-2%, penicillin with the concentration being 100 U / mL, streptomycin with the concentration being 100 [mu] g / mL, OA with the concentration being 60-150 [mu] mol / L, EPA with the concentration being 40-80 [mu] mol / L, DHA with the concentration being 20-100 [mu] mol / L, insulin with the concentration being 5-10 [mu] g / mL, transferrin with the concentration being 10-20 [mu] g / mL and glutamine with the concentration being 2-4 mmol / L. The adipogenic differentiation effect of the adipogenic induction culture medium is good, the cell differentiation efficiency reaches 100%, the fat maturity is obviously improved, and the repeatability is good.