97 results about "L-alanyl-l-glutamine" patented technology

The invention provides a feedstuff for sturgeon and hucho taimen at mouth - open period, which solves the problems of the conventional feedstuff that the growth rates of the sturgeon and hucho taimen are slow and the survival rates are low. The feedstuff comprises: dextrin, full cream milk powder, blood powder, white fish meal, soy protein, L-alanyl-L-glutamine, methylol cellulose and ore-mixing agent. When the feedstuff is used the for sturgeon and hucho taimen at mouth - open period, the growth rates and the survival rates of the sturgeon and hucho taimen are increased and the special growth rates of the sturgeon and hucho taimen are 1.5%-6.2% and the survival rates are more than 98%.

The invention provides a bio-enzyme for synthesis of proglumetacin dipeptide N-(2)-L-alanyl-L-glutamine by catalysis as well as a preparation method and an application thereof. The amino acid sequence of the bio-enzyme is shown as SEQID NO: 2. The preparation method of the bio-enzyme comprises the following steps: firstly, constructing a genetically engineered strain of the bio-enzyme, and recombining plasmids by cutting the gene segment of the bio-enzyme linearly by using a restriction endonuclease SalI, wherein the gene segment of the bio-enzyme is totally synthesized by genes; and then selecting a positive clone, adding the positive clone into a YPD liquid culture medium, activating, inoculating the positive clone in a BMGY liquid culture medium, culturing the positive clone until the OD reaches 1, inoculating the positive clone in 1% methanol, inducing for 72 hours to express the bio-enzyme, and further fermenting to obtain the bio-enzyme for synthesis of proglumetacin dipeptide N-(2)-L-alanyl-L-glutamine by catalysis. The synthesis of N-(2)-L-alanyl-L-glutamine from bio-enzyme by catalysis is as follows: heating to deactivate, filtering, removing the generated salt by adopting a nanofiltration technology, concentrating, and crystallizing with methanol and water to obtain the high-purity N-(2)-L-alanyl-L-glutamine. The synthesis method of N-(2)-L-alanyl-L-glutamine is simple, efficient and environment-friendly and has extremely high economic value and market competitiveness.

The invention discloses a preparing method of N(2)-L-alanyl-L-glutamine, which comprises the following steps: producing precursor acid potassium; producing mixed acid anhydride; producing glutamine sodium salt; producing N(2)-L-alanyl-L-glutamine (ala paddy dipeptide); refining. This invention possesses the advantages of simple craft, convenient operation and little contamination.

The invention relates to an artificial particle feed for juvenile turbot. The artificial particle feed is characterized by being prepared from whitefish powder, euphausiid powder, scallop powder, beer yeast, starch, cod liver oil, yolk powder, whey powder, L-alanyl-L-glutamine, choline chloride, soybean lecithin, schizochytrium limacinum powder, haematococcus pluvialis powder, compound vitamins, compound mineral substances, Chinese herbal medicines and sodium alginate. As the various raw materials are added into the feed, the comprehensiveness of nutrition is guaranteed. An immunopotentiator is added into the feed, so that the non-specificity immunity of fish bodies is enhanced. The feed is green and pollution-free. The schizochytrium limacinum powder and the haematococcus pluvialis powder are added into the feed, and the content of vitamin E is improved, so that the whitening rate of the juvenile fish is effectively reduced. The Chinese herbal medicine components are added, so that the immunity of the juvenile fish can be improved and parasite diseases in a juvenile fish growing phase are prevented from happening.

The invention discloses a method for preparing N(2)-L-alanyl-L-glutamine, belonging to a method for preparing dipeptide containing four amino acids at most. The method comprises the following steps: (1) inorganic base is added into the solution of toluene and water which contains L-glutamine; then the mixture of D-alpha-chloropropionyl chloride and toluene is added and the pH value of reaction solution is controlled between 9.5 and 10.5 by the inorganic base; the reaction temperature is maintained between 0-5 DEG C; the reaction solution is quiescent so that a toluene layer is separated; sodium chloride is added into the remaining solution at room temperature and N(2)-D-alpha-chloropropionyl-L-glutamine is obtained after filtration and drying; ammonia is added into the N (2)-D-alpha-chloropropionyl-L-glutamine, and concentrated solution after concentration under reduced pressure is transferred to a crystallizer; methanol is added and a crude product is obtained after filtration and drying; a refined product of the N (2)-L-alanyl-L-glutamine is obtained by refining.

An aseptic powder of N(2)-L-alanyl-L-glutamine is prepared through adding N(2)-L-alanyl-L-glutamine to distilled water in aseptic condition, stirring, heating for dissolving, adding medical activated carbon, stirring, filtering for removing activated carbon, millipore filtering for removing bacteria, adding aseptic alcohol to educe out crystals, filtering, washing with cold alcohol, and vacuum drying. It has high optical and thermal stability.

The present invention relates to synthesis of dipeptide containing amino acid, and provides a kind of synthesis process of N(2)-L-alanyl-L-glutamine dipeptide with low material cost, simplicity, high yield, no need of separating and purifying intermediate product, easy product separation and purification and environment friendship. The synthesis process includes the reaction of amino acid with protected N-terminal with phosphorus triphenyl oxide and triphosgene in organic solvent to form active ester; the reaction of the active ester with glutamine in water solution of inorganic alkali; acidifying with inorganic acid and eliminating N-terminal protecting radical.

The invention provides a N(2)-L-alanyl-glutamine for injection and its preparing method, it has mixed N(2)-L-alanyl-glutamine and mannite to make N(2)-L-alanyl-glutamine whose mess percentage density is 50-100%. The invention has increased the stability of medicine greatly, convenient for transportation and storage, suitable for sicker in catabolism and hypermetabolism condition who needs glutamine.

The invention discloses a separation and purification method of N(2)-L-alanyl-L-glutamine, which is characterized by comprising the following steps: 1. dezymotization by ceramic membrane filtration: filtering a N(2)-L-alanyl-L-glutamine enzyme reaction solution through a ceramic membrane filter plant while washing with water, and removing the residual zymoprotein in the reaction solution, thereby obtaining a ceramic membrane dialysate; 2. continuous chromatography desalting: sending the ceramic membrane dialysate into a continuous chromatography separation plant to perform continuous chromatography separation so as to separate the N(2)-L-alanyl-L-glutamine from inorganic salts, thereby obtaining a N(2)-L-alanyl-L-glutamine solution; and 3. evaporation and crystallization: carrying out vacuum distillation on the N(2)-L-alanyl-L-glutamine solution obtained by continuous chromatography, and crystallizing to obtain the N(2)-L-alanyl-L-glutamine product. The method lowers the production cost, simplifies the production technique, shortens the production cycle, enhances the total yield, and has the advantages of high product purity and the like.

The invention relates to a large-scale preparation method of mesenchymal stem cell factors. The large-scale preparation method comprises three steps of invitro culture of mesenchymal stem cells, induction and secretion of the mesenchymal stem cell factors and separation and purification of the mesenchymal stem cell factors, wherein an induction medium required by the step of the induction and secretion of the mesenchymal stem cell factors is prepared from a mixed medium of a DMEM (Dulbecco's Modified Eagle Medium), a PBS buffer solution and an L-alanyl-L-glutamine aqueous solution of which the volume ratio is (30 to 80):(20 to 60):(0.2 to 1.5) and an inducer. According to the large-scale preparation method of the mesenchymal stem cell factors, a single sample source is adopted to culture on a large scale, so that the uniformity of batch products is guaranteed, and the induction medium is adopted in the step of the induction and secretion of the mesenchymal stem cell factors, so that a large quantity of cell factors are stimulated to secrete, a formula is reasonable and effective, the conditions of the whole preparation process are stable and reasonable, and the large-scale preparation method is suitable for large-scale production.

The invention belongs to the technical field of medicines, and particularly relates to a preparation method of N(2)-L-alanyl-L-glutamine. The preparation method of the N(2)-L-alanyl-L-glutamine comprises the following steps of (1) preparing L-phthaloyl-alanyl chloride; (2) preparing phthaloyl-L-alanyl-L-glutamic acid; (3) preparing phthaloyl-L-alanyl-L-glutamic acid anhydride; (4) preparing phthaloyl-L-alanyl-L-glutamine; (5) preparing an N(2)-L-alanyl-L-glutamine crude product; (6) preparing an N(2)-L-alanyl-L-glutamine refined product. The product obtained through the final deprotection process of the preparation method as a pilot plant test or a production scale process route is higher in purity; the liquid-phase purity of the product is higher than 99.9% through primary purification, and the product is low in impurity content.

The invention discloses a nucleic acid protective liquid and an application thereof for long-term storage and transportation of a tissue sample at constant temperature. The nucleic acid protective liquid comprises the following components: D-glucose, 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid, inorganic salt, amino acids, choline chloride, folic acid, nicotinamide, inositol, vitamin A, vitamin H, vitamin E, Holo-human transferrin, bovine serum albumin, N-(2)-L-alanyl-L-glutamine, insulin, adrenalone, linoleic acid, linolenic acid, progesterone, penicillin, streptomycin, amphotericin B and water. The nucleic acid protective liquid is low in cost. By using the nucleic acid protective liquid, nucleic acid in the tissue sample is long in storage time at room temperature, and the obtained nucleic acid is high in concentration and great in total amount. A preparation method for the nucleic acid protective liquid is simple and fast.

The invention discloses an N(2)-L-alanyl-L-glutamine preparation for injection and a preparation method thereof. The preparation is prepared through directly preparing a liquid with a raw material N(2)-L-alanyl-L-glutamine, freeze-drying, and carrying out separate packing. The method comprises the following steps: (1) taking the raw material N(2)-L-alanyl-L-glutamine, adding water for injection, and fully stirring to completely dissolve N(2)-L-alanyl-L-glutamine; (2) adding needle active carbon to a solution obtained in step (1), fully stirring, filtering to decarburize, and carrying out germ filtering through passing through a filter with a diameter of 0.2 [mu]m; (3) putting a filtrate obtained in step 2 into a sterilized freeze-dried disc, loading the disc into a cooling chamber of a freeze dryer, and carrying out pre-freezing, vacuumizing, heating, sublimation drying, desorption drying, and vacuum break to end freeze-drying; and (4) accessing high purity nitrogen for drying and degerming, taking out of the chamber, carrying out asepsis packing, tamponading, and capping. So N(2)-L-alanyl-L-glutamine for injection is obtained.

The invention discloses a serum-free medium for culturing immune cells. The medium includes a basic medium, bovine serum albumin, fatty acids, cholesterols, insulin, transferrin, 2-mercaptoethanol, N-(2)-L-alanyl-L-glutamine and glyceride. The serum-free medium has the advantages of simple component, stable properties and high CIK induction efficiency, and CIK cells cultured through the medium have the advantages of small batch difference, stable quality, convenient quality control, and reduction of accidental infection of patients, and are of great significance to promoting and applying immunotherapy.

The invention provides an in vitro culture medium for culturing human embryos, and a preparation method of the in vitro culture medium, belonging to the field of human assisted reproduction. The in vitro culture medium is mainly prepared from the following components: 0.01-0.05g / L of gentamicin sulfate, 0.005-0.01g / L of phenol red, 0-0.01mM / L of disodium ethylenediaminetetraacetate, 90-116mM / L ofsodium chloride, 2.5-10.0 mM / L of potassium chloride, 0-1.8 mM / L of calcium chloride dihydrate, 0-7.16 mM / L of potassium dihydrogen phosphate, 0.2-1.51M / L of magnesium sulfate dihydrate, 20-25 mM / L of sodium bicarbonate, 0.1-7.27 mM / L of sodium pyruvate, 0-20 mM / L of sodium lactate, 0-3.25 mM / L of glucose, 0-1.0 mM / L of sodium citrate, 0-1.0mM / L of L-alanyl-L-glutamine, 0-2.7mM / L of inositol, 0.05-5mM / L of taurine, 5g / L of human serum protein, essential amino acids and non-essential amino acids.

The invention discloses a method of producing L-alanyl-L-glutamine from recombinant escherichia coli, wherein the method of producing L-alanyl-L-glutamine from recombinant escherichia coli is follows: in an aqueous solution with a determined pH value, acting on free L-glutamine and L-alanine methyl ester hydrochloride to generate L-alanyl-L-glutamine, recombining a gene segment of the protein with the amino-acid ester acyltransferase of the L-alanyl-L-glutamine into a carrier and transferring into host bacteria, thereby obtaining the host bacteria with the recombinant DNA and capable of strengthening the activity of an L-alanyl-L-glutamine biological synthesis system. The method comprises the following steps: (1) culturing a larger number of recombinant escherichia coli cells for expressing the amino-acid ester acyltransferase; (2) excessively expressing the amino-acid ester acyltransferase in the step (1); and (3) taking the amino-acid ester acyltransferase in the step (2) as a crude enzyme source, adding the crude enzyme source into a buffer solution containing L-glutamine and L-alanine methyl ester hydrochloride substrate amino acid to react, thereby realizing efficient production of the L-alanyl-L-glutamine.

The invention discloses an HPLC (high performance liquid chromatography) detection method for simultaneously determining five substances (L-Ala-L-Gln (L-alanyl-L-glutamine), L-Gln, L-AlaOMe, L-Glu and L-alanyl-L-alanine) in a reaction system for producing L-Ala-L-Gln with a microbial enzyme method. The five substances are subjected to pre-column automatic derivatization treatment by ortho-phthaladehyde, so that the five substances have fluorescence groups and are detected by a fluorescence detector. The chromatographic condition is as follows: a high performance liquid chromatograph Agilent 1260 Infinity is adopted; an Agilent ZORBAX SB-Aq, 5 mu m, 4.6*250 mm C18 column is adopted as a chromatographic column; an Agilent 1260 Infinity fluorescence detector is adopted; a mobile phase comprises acetonitrile and a phosphate buffer solution in the volume ratio being 12:88, and the pH of the mobile phase is adjusted to 7.4 by the aid of phosphoric acid; the flow velocity of the mobile phase is 1.0 ml / min; the column room temperature is 40 DEG C; detection wavelength comprises excitation wavelength Ex 338 nm and emission wavelength Em 450 nm. The method realizes simultaneous determination of oligopeptide, amino acid and amino derivatives, is simple to operate and high in sensitivity and has important application value.

The invention relates to artificial pellet feed for adult turbot. The artificial pellet feed is characterized by comprising white fish meal, red fish meal, whey protein, soybean protein concentrate, soybean meal hydrolysate small peptide, scallop powder, chicken meal, high protein flour, alpha-starch, L-alanyl-L-glutamine, choline chloride, sea fish oil, soybean lecithin, krill meal, ocean red yeast, photosynthetic bacteria, Schizochytrium limacinum powder, haematococcus pluvialis powder, multivitamins, composite mineral substances, Chinese herbal medicines and sodium alginate. According to the feed, a plurality of kinds of raw materials are added into the feed, so that the comprehensiveness of nutrition is guaranteed. During actual culture of the turbot, the pellet feed for the adult turbot has the advantages that the feed is good in feeding promoting performance, fishes like the feed, are full easily and do not fall ill easily, the survival rate is high, and the feed coefficient is low; the digestibility and the feed conversion ratio are high; the growth rate of the fishes is high, and the culture cycle is short.

A composition used as gastric mucosa protecting agent for treating acute or chronic gastritis, gastric ulcer, duodenal ulcer, etc is prepared from the water-soluble precursors including sodium azulenesulfonate and L-glutamine or glycyl-L-glutamine or L-alanyl-L-glutamine through proportioning and conventional steps.

The invention relates to an artificial microparticle feed of turbot postlarva, and the artificial microparticle feed is characterized by including the following feed components: white fish meal, krill meal, squid meal, brewer yeast, starch, cod liver oil, egg yolk powder, whey powder, L-alanyl-L-glutamine, choline chloride and soy lecithin. The artificial microparticle feed may also include the following feed components: schizochytrium aggregatum powder, a compound vitamin, a compound mineral, gelatin and sodium alginate. A plurality of raw materials are added into the artificial microparticle feed to ensure comprehensive nutrition, immunopotentiators are added into the artificial microparticle feed to enhance fish-body non-specific immunity, the artificial microparticle feed is pollution-free and green, and the schizochytrium aggregatum powder is added into the artificial microparticle feed for effective reduction of the fry albino rate.

The invention provides an N-(2)-L-alanyl-L-glutamine (molecular formula: C8H15N3O4) III-type crystalline compound which has better solubleness and stability. Meanwhile, the invention also provides a preparation method for a new crystal including medicine composition and preparation agent thereof, particularly an injection.

The invention discloses a method for preparing an N-(2)-L-alanyl-L-glutamine compound. The N-(2)-L-alanyl-L-glutamine compound is prepared by adopting a particle process crystal product molecule assembly and form optimization technique. The compound has the characteristics of high purity, high yield, low hygroscopicity and good stability. Meanwhile, the invention also discloses a pharmaceutical composition prepared from the alanyl glutamine prepared by the method. The pharmaceutical composition is prepared through the steps of preparing the alanyl glutamine into a 10-30% aqueous solution, adsorbing the aqueous solution with medicinal activated charcoal according to the ratio (w / v) of 0.1-0.5%, then, carrying out big-dish freeze-drying, and carrying out aseptic packaging. The whole preparation process is simple in operation and does not need any excipient, and the product has better stability compared with the products in the past.

The invention discloses a solid beverage containing N(2)-L-alanyl-L-glutamine, which is prepared from the following raw materials: N(2)-L-alanyl-L-glutamine, sucrose, taurine, arginine aspartate, lysine hydrochloride, ornithine hydrochloride, vitamin B3, vitamin B6, vitamin C, vitamin B12, sodium citrate, glucuronolactone, potassium chloride, DHA (docosahexaenoic acid) and sweetening agent. The solid beverage can supplement energy, enhance vigor and resist senility and fatigue, is composed of amino acids, vitamins, additives, microelements and the like, and is a functional beverage preparation for regulating the human functions; and the solid beverage is suitable for travel enthusiasts, athletes, manual workers and brain workers to drink.

The invention discloses a secretion inducing medium for large-scale preparation of human umbilical cord mesenchymal stem cell factors. The secretion inducing medium is mainly prepared by dissolving DMEM (Dulbecco's modified eagle medium), L-alanyl-L-glutamine, 4-2-ethoxyl-1-piperazine ethanesulfonic acid, D-glucose and L-ascorbic acid inside phosphate buffer, wherein the concentrations of the DMEM, the L-alanyl-L-glutamine, the 4-2-ethoxyl-1-piperazine ethanesulfonic acid, the D-glucose and the L-ascorbic acid inside the secretion inducing medium are, respectively, 400-600 ml / L, 1-3 mmol / L, 10-20 mu mol / L and 10-100 mu mol / L. The secretion inducing medium for large-scale preparation of the human umbilical cord mesenchymal stem cell factors is applicable to large-scale cultivation of human umbilical cord mesenchymal stem cells and can efficiency induce secretion of active factors.

The invention relates to a temozolomide freeze-dried preparation, which contains a temozolomide active ingredient and at least one solubilizer which can dissolve the temozolomide. The solubilizer used in the preparation may be N(2)-L-Alanyl-L-glutamine, glycyl-L-glutamine monohydrate, glycyl-l-tyrosine, ornithine aspartate or a mixture of the four. The temozolomide preparation prepared by using the technology provided by the invention has the advantages that the solubility of the temozolomide and the stability of the solution are improved; the quality of the solution is stable; the use of thepreparation is convenient; and the preparation method is simple and suitable for large-scale preparation.

The invention discloses a culture solution for culturing 293T cells in a serum-free suspension mode. According to the culture solution for culturing the 293T cells in the serum-free suspension mode, ahigh-glucose DEMEM-F12 culture substrate without L-glutamine serves as a base solution, and L-alanyl-L-glutamine, vitamin C, HEPES, an insulin-like growth factor, an epidermal growth factor, lipid concentrate, human serum albumin, transferrin, trehalose, heparin sodium and Pluronic F-68 are added. The culture solution for culturing the 293T cells in the serum-free suspension mode contains clear components without any serum components, is explicit in component, stable in batch, controllable in quality, low in cost and suitable for not only routine culture in laboratories but also large-scale production culture, and has broad application prospects.

The invention discloses a composite amino acid vitamin injection and the application thereof. Every 1000ml of the composite amino acid vitamin injection comprises 5-40g of L-leucine, 2-30g of L-isoleucine, 2-30g of L-valine, 5-90g of L-alanyl-L-glutamine, 1-5g of L-threonin 1-10g of L-lysine, 1-12g of L-methionine, 1-10g of L-phenylalanine, 0.5-8g of L-tryptophan, 2-30g of L-arginine, 1-10g of L-histidine, 1-100mg of vitamin B6, 0-50mg of vitamin B1 and 0-100mu g of vitamin B12. Aiming at variation of stress, immunity, metabolism and the like of a human body in a perioperative anesthesia period, the composite amino acid vitamin injection achieves the purposes of inhibiting catabolism, promoting anabolism, generating heat and keeping warm, improving body substance energy metabolism and immunity functions and accelerating heal through creative formulae on the basis of enhanced recovery after surgery.

The invention belongs to the field of medicine, and provides a compound amino acid dipeptide injection, a preparation method thereof and an application thereof, the injection comprises three cavity bags, the three cavity bags are respectively separated by three bags and filled with a branched-chain amino acid solution, a L-alanyl-L-glutamine solution, and a mixed solution containing arginine hydrochloride and vitamin B6; wherein, in every 1000 mL of the injection solution, the total mass of the amino acid is 50 g to 130 g; and the branched chain amino acid mass accounts for 10 to 80% of the total mass of the amino acid; the mass of L-alanyl-L-glutamine is 5 to 70% of the total mass of the amino acid; the mass of arginine hydrochloride is 0 to 50% of the total mass of the amino acid; and the mass of the vitamin B6 is 0 to 1 g. The injection of the invention improves the stability and effectiveness of the product while providing product safety, and the technical scheme is simple and convenient for industrial production.

The preparation process of L-alanyl-L-glutamine includes the first condensation reaction between D-2-halopropionic acid and glutamine inside inert solvent and in the presence of condensing agent and acidification to obtain D-2-halopropionyl glutamine; and the aminolysis reaction of the obtained D-2-halopropionyl glutamine with ammonia water to obtain L-alanyl-L-glutamine. The preparation process of the present invention is simple, and has the advantages of less reaction steps, less pollution, facile material, easy separation of the final product, low production cost, short production period and high product yield.

The invention discloses a method for improving activity of an L-alanyl-L-glutamine biosynthesis system containing recombinant DNA. A recombinant plasmid of the recombinant escherichia coli comprises an amino-acid ester acyltransferase encoding gene (SAET) which can catalyze L-glutamine and L-alanine methyl ester hydrochloride to generate L-alanyl-L-glutamine, and a multidrug efflux transporter protein encoding gene (ydeE) which can transporting L-alanyl-L-glutamine outside cells from cells, and due to co-expression of the two encoding genes, stability of a bacterium can be effectively improved, and the yield and the transformation rate of the bacterium can be increased. The recombinant plasmid is obtained by recombining SAET and ydeE with the same plasmid, or by co-transforming two recombinant plasmids with different resistances of two genes respectively. The yield of L-alanyl-L-glutamine of the recombinant bacterium can be increased by 3.33 times when compared with a conventional yield.