Secretion inducing medium for large-scale preparation of human umbilical cord mesenchymal stem cell factors

A technology for large-scale preparation of stromal cells, which is applied in the field of large-scale preparation of induced secretion medium for human umbilical cord mesenchymal stem cells, which can solve the problem of insufficient utilization of active factors, small culture scale, and inefficient separation and purification process. And other issues

A technology for large-scale preparation of stromal cells, which is applied in the field of large-scale preparation of induced secretion medium for human umbilical cord mesenchymal stem cells, which can solve the problem of insufficient utilization of active factors, small culture scale, and inefficient separation and purification process. And other issues

CN106497873AActive Publication Date: 2017-03-15北京银丰鼎诚生物工程技术有限公司
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  • Secretion inducing medium for large-scale preparation of human umbilical cord mesenchymal stem cell factors

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Induced secretion medium for large-scale preparation of human umbilical cord mesenchymal stem cell factors, mainly composed of DMEM, L-alanyl-L-glutamine, 4-2-hydroxyethyl-1-piperazineethanesulfonic acid, D- Glucose and L-ascorbic acid dissolved in phosphate buffer, DMEM, L-alanyl-L-glutamine, 4-2-hydroxyethyl-1-piperazineethanesulfonic acid, D-glucose and L- The concentrations of ascorbic acid in the induced secretion medium were 400ml / L, 1mmol / L, 10μmol / L, 10g / L, 10μmol / L, respectively.

Embodiment 2

[0029] Induced secretion medium for large-scale preparation of human umbilical cord mesenchymal stem cell factors, mainly composed of DMEM, L-alanyl-L-glutamine, 4-2-hydroxyethyl-1-piperazineethanesulfonic acid, D- Glucose and L-ascorbic acid dissolved in phosphate buffer, DMEM, L-alanyl-L-glutamine, 4-2-hydroxyethyl-1-piperazineethanesulfonic acid, D-glucose and L- The concentrations of ascorbic acid in the induced secretion medium were 600ml / L, 3mmol / L, 20μmol / L, 20g / L, 100μmol / L, respectively.

Embodiment 3

[0031] Induced secretion medium for large-scale preparation of human umbilical cord mesenchymal stem cell factors, mainly composed of DMEM, L-alanyl-L-glutamine, 4-2-hydroxyethyl-1-piperazineethanesulfonic acid, D- Glucose and L-ascorbic acid dissolved in phosphate buffer, DMEM, L-alanyl-L-glutamine, 4-2-hydroxyethyl-1-piperazineethanesulfonic acid, D-glucose and L- The concentrations of ascorbic acid in the induced secretion medium were 500ml / L, 2mmol / L, 15μmol / L, 15g / L, 50μmol / L, respectively.

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Abstract

The invention discloses a secretion inducing medium for large-scale preparation of human umbilical cord mesenchymal stem cell factors. The secretion inducing medium is mainly prepared by dissolving DMEM (Dulbecco's modified eagle medium), L-alanyl-L-glutamine, 4-2-ethoxyl-1-piperazine ethanesulfonic acid, D-glucose and L-ascorbic acid inside phosphate buffer, wherein the concentrations of the DMEM, the L-alanyl-L-glutamine, the 4-2-ethoxyl-1-piperazine ethanesulfonic acid, the D-glucose and the L-ascorbic acid inside the secretion inducing medium are, respectively, 400-600 ml / L, 1-3 mmol / L, 10-20 mu mol / L and 10-100 mu mol / L. The secretion inducing medium for large-scale preparation of the human umbilical cord mesenchymal stem cell factors is applicable to large-scale cultivation of human umbilical cord mesenchymal stem cells and can efficiency induce secretion of active factors.

Description

technical field [0001] The invention belongs to the field of medium for mesenchymal stem cells, in particular to an induced secretion medium for large-scale preparation of human umbilical cord mesenchymal stem cell factors. Background technique [0002] Mesenchymal stem cells are derived from the mesoderm and ectoderm in the early stages of development and belong to pluripotent stem cells. It has attracted increasing attention because of its multi-directional differentiation potential, hematopoietic support and promotion of stem cell implantation, immune regulation and self-replication. Mesenchymal stem cells were first discovered in the bone marrow, and later found in many kinds of tissues in the process of human development and development. At present, we can isolate and prepare mesenchymal stem cells from bone marrow, fat, umbilical cord, synovium, bone and other tissues, among which the bone marrow-derived mesenchymal stem cells were studied the earliest. However, bone...

Claims

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Application Information

Patent Timeline
15 Mar 2017
Publication
CN106497873A
IPC
C12N5/0775
Inventors
雷云霆; 董白翔