Secretion inducing medium for large-scale preparation of human umbilical cord mesenchymal stem cell factors

A technology for large-scale preparation of stromal cells, which is applied in the field of large-scale preparation of induced secretion medium for human umbilical cord mesenchymal stem cells, which can solve the problem of insufficient utilization of active factors, small culture scale, and inefficient separation and purification process. And other issues

Active Publication Date: 2017-03-15
北京银丰鼎诚生物工程技术有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

When cultivating mesenchymal stem cells to collect culture fluid or cell lysate, the culture scale is small, the process of inducing cytokine secretion is not efficient enough, the separation and purification process is not scaled, and the secreted active factors are not fully utilized.

Method used

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  • Secretion inducing medium for large-scale preparation of human umbilical cord mesenchymal stem cell factors

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Effect test

Embodiment 1

[0027] Induced secretion medium for large-scale preparation of human umbilical cord mesenchymal stem cell factors, mainly composed of DMEM, L-alanyl-L-glutamine, 4-2-hydroxyethyl-1-piperazineethanesulfonic acid, D- Glucose and L-ascorbic acid dissolved in phosphate buffer, DMEM, L-alanyl-L-glutamine, 4-2-hydroxyethyl-1-piperazineethanesulfonic acid, D-glucose and L- The concentrations of ascorbic acid in the induced secretion medium were 400ml / L, 1mmol / L, 10μmol / L, 10g / L, 10μmol / L, respectively.

Embodiment 2

[0029] Induced secretion medium for large-scale preparation of human umbilical cord mesenchymal stem cell factors, mainly composed of DMEM, L-alanyl-L-glutamine, 4-2-hydroxyethyl-1-piperazineethanesulfonic acid, D- Glucose and L-ascorbic acid dissolved in phosphate buffer, DMEM, L-alanyl-L-glutamine, 4-2-hydroxyethyl-1-piperazineethanesulfonic acid, D-glucose and L- The concentrations of ascorbic acid in the induced secretion medium were 600ml / L, 3mmol / L, 20μmol / L, 20g / L, 100μmol / L, respectively.

Embodiment 3

[0031] Induced secretion medium for large-scale preparation of human umbilical cord mesenchymal stem cell factors, mainly composed of DMEM, L-alanyl-L-glutamine, 4-2-hydroxyethyl-1-piperazineethanesulfonic acid, D- Glucose and L-ascorbic acid dissolved in phosphate buffer, DMEM, L-alanyl-L-glutamine, 4-2-hydroxyethyl-1-piperazineethanesulfonic acid, D-glucose and L- The concentrations of ascorbic acid in the induced secretion medium were 500ml / L, 2mmol / L, 15μmol / L, 15g / L, 50μmol / L, respectively.

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Abstract

The invention discloses a secretion inducing medium for large-scale preparation of human umbilical cord mesenchymal stem cell factors. The secretion inducing medium is mainly prepared by dissolving DMEM (Dulbecco's modified eagle medium), L-alanyl-L-glutamine, 4-2-ethoxyl-1-piperazine ethanesulfonic acid, D-glucose and L-ascorbic acid inside phosphate buffer, wherein the concentrations of the DMEM, the L-alanyl-L-glutamine, the 4-2-ethoxyl-1-piperazine ethanesulfonic acid, the D-glucose and the L-ascorbic acid inside the secretion inducing medium are, respectively, 400-600 ml/L, 1-3 mmol/L, 10-20 mu mol/L and 10-100 mu mol/L. The secretion inducing medium for large-scale preparation of the human umbilical cord mesenchymal stem cell factors is applicable to large-scale cultivation of human umbilical cord mesenchymal stem cells and can efficiency induce secretion of active factors.

Description

technical field [0001] The invention belongs to the field of medium for mesenchymal stem cells, in particular to an induced secretion medium for large-scale preparation of human umbilical cord mesenchymal stem cell factors. Background technique [0002] Mesenchymal stem cells are derived from the mesoderm and ectoderm in the early stages of development and belong to pluripotent stem cells. It has attracted increasing attention because of its multi-directional differentiation potential, hematopoietic support and promotion of stem cell implantation, immune regulation and self-replication. Mesenchymal stem cells were first discovered in the bone marrow, and later found in many kinds of tissues in the process of human development and development. At present, we can isolate and prepare mesenchymal stem cells from bone marrow, fat, umbilical cord, synovium, bone and other tissues, among which the bone marrow-derived mesenchymal stem cells were studied the earliest. However, bone...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
Inventor 雷云霆董白翔
Owner 北京银丰鼎诚生物工程技术有限公司
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