1 results about "Ph buffering" patented technology

The present application relates to the technical field of agricultural product quality and safety detection and element form analysis, and particularly relates to an LC-ICP-MS / MS method for detecting selenocysteine. The present application provides an LC-ICP-MS / MS method for detecting selenocysteine, wherein the sample to be detected is mixed with iodine acetamide solution and buffer extraction solution for enzymolysis to obtain an enzymolysis solution; the final concentration of iodine acetamide in the mixed solution is 0.02-0.10 mmol / L; the purified solution is injected into a liquid chromatography system for high performance liquid chromatography separation, a solution without ion pair reagent, with pH buffering capacity and excellent ICP-MS compatibility is used as a mobile phase, and an anion exchange chromatographic column is used as a chromatographic column; the chromatographic effluent is introduced into ICP-MS / MS for detection, CH4 is used as a reaction gas, a detection method with a recovery rate of greater than or equal to 90% and no residual interference is constructed, and a new paradigm is provided for the accurate analysis of unstable selenium forms.