This invention provides a 2-pyridinone-based
afterglow chemiluminescent molecule, its preparation method, and its application. The Chinese name of the
afterglow chemiluminescent molecule is (…). E )-2-(2-(6-((4-
boronic acid benzyl)oxy)-7-fluoro-2,3-dihydro-1 H -oxanthracene-4-yl)vinyl)-3,3-dimethyl-1-(4-(3-methyl-2-oxopyridine-1-(2-yl)- ... H )-butyl)-3 H -Indole-1-
onium. This invention also provides a method for preparing this
afterglow chemiluminescent molecule. After being embedded in Pluronic F127 to form nanoparticles, the molecule exhibits excellent afterglow
luminescence performance upon activation with oxynitrite in PBS buffer. This invention overcomes the challenges of irreversible substrate consumption, blue shift of emission, and low
energy transfer efficiency in traditional organic afterglow systems by using 2-pyridone as a stable, non-consumable substrate and covalently linking it to the luminescent unit, providing a powerful new platform for precise molecular sensing and further
disease diagnosis.