144 results about "Alizarin" patented technology

The present invention provides an electroplating method of magnesium and magnesium alloy. The technology comprises dehydrogen, chemical degreasing, shining, neutralization, activation, pre-plating, functional plating, decorative plating and post treatment. The cast workpiece must be water-washed between every two procedures. The current density of pre-plating is 5-15 A / dm2, cell voltage 4-12 V, pH value of pre-plating solution 1.0-1.2, and Baume degree at room temperature is 30-35. Pre-plating solution recipe contains: copper fluoborate, fluoboric acid, alanine, polyethylene glycol and alizarin dye, mixture of itaconic acid and MBT, 3-S isothiourea onium salt propane sulfonate ,R-S-S(CH2)SO3Na and deionization water. The pre-plating solution of the present invention is cyanogen free, non-toxic and non-hazardous. Bond strength between pre-plating layer and substrate material and between pre-plating layer and the post functional plating layer or decorative plating layer is good. The plating layer is bright and has good corrosion resistance, wear resistance and decorative effect.

The invention provides an flow battery negative electrode electrolyte based on alizarin or derivatives thereof, and a flow battery adopting the electrolyte as a negative electrode. The electrolyte is an alkaline aqueous solution containing the alizarin or the derivatives thereof. The alizarin and the derivatives contain at least one of alizarin (1,2-dihydroxyanthraquinone), alizarin red (1,2-dihydroxyanthraquinone-3-sulfonic acid) or alizarin fluorin blue (3-alizarin methylamine-N,N-diacetic acid and mixtures thereof. An alkali used in the invention comprises at least one of sodium hydroxide, potassium hydroxide and a sodium hydroxide and potassium hydroxide mixture. The working temperature of the electrolyte is 10-50 DEG C; and the flow battery based on the electrolyte is a traditional structure, and comprises a positive electrode liquid storage tank 1, a positive electrode electrolyte 3, a negative electrode liquid storage tank, the negative electrode electrolyte 4, pumps 7 and 8, and a cation exchange membrane 9 for conducting cations. The active substance of the alizarin flow battery negative electrode electrolyte is bulk anions, and difficultly penetrates through a proton exchange membrane; and the electrolyte disclosed in the invention has the characteristics of no toxicity, no pollution, no rare earth elements, and obvious price advantage.

The invention provides a chinlon dyeing process adopting natural dye. The method comprises the steps of pretreatment, presetting, dyeing, soaping, fixation, softening treatment, drying, setting and rolling. The natural dye, alizarin, is adopted, rare earth chloride is adopted as dye mordant, the dyeing effect is better, the washability is greatly enhanced, and meanwhile dyed chinlon fibers have the good antibacterial property. The washing and fixation process is added, the dyeing firmness is good, environment-friendly formaldehyde-free TCD-R is adopted in the fixation process, chinlon is dried step by step after fixation, and the firmness of the chinlon is greatly improved. The softening treatment process is added, the color fastness and softness of fabric are improved, the roughness of the fabric is reduced, and the fabric is fluffy and elastic.

The invention relates to a method for marking gold sepia, wherein the invention is characterized in that: cultivating the hatched sepia for 5-20 days, dissolving coloring agent into fresh water, adding the water solution into the container with sepia and seawater, to make its coloring agent content as 60-80ppm; emerging and coloring for 20-28hours; said coloring agent is alizarin complex instructor. The invention has simple process without toxic and hurt, while it can color many types of sepia at one time.

The invention discloses a method for detecting amoxicillin residual quantity in milk and meat products. The method includes the following steps: preparing amoxicillin standard aqueous solution; adding a nonionic surfactant; reacting the obtained solution with quinone or alizarin to get a charge transfer system; determining the wavelength scanning range of fluorescence or ultraviolet; further calculating the linear range, working curve and other parameters of the standard solution; processing the sample to be detected by corresponding charge transfer reaction; determining reaction parameters under ultraviolet or fluorescence; and calculating to obtain the amoxicillin residue quantity. The technology further enhances the value of fluorescence intensity or UV absorption by utilizing the sensitizing effect of non-ionic surfactants, thus meets the low-residue testing requirement; simultaneously improves the system stability significantly, makes up the disadvantage of poor detection stability of fluorescence in the earlier application, and is more feasible in the actual detection.

The invention relates to madder extract and a preparation method and an application thereof, wherein the madder extract is prepared from the following steps: pulverizing the rhizome of the madder, and using ethanol for percolation extraction at room temperature; recovering the ethanol and obtaining extract; dissolving the extract into water, and heating and completely dissolving the extract; and then, extracting the extract by using ethyl acetate, and concentrating the extract to obtain the madder ethyl acetate extract. The experiment indicates that the madder ethyl acetate extract and the compound alizarin can effectively reduce the blood glucose value, the GSP value, the TG value and the content of MDA in the tissue homogenate of the mouse with hyperglycemia caused by alloxan, and can obviously increase the SOD activity value of the hepatocyte of the mouse and increase the content of the hepatic glycogen of the tissue. The results prove that the madder ethyl acetate extract and the alizarin have good effect on reducing the content of the blood glucose.

The invention relates to the technical field of assay determination of anthracene of polycyclic aromatic hydrocarbon, and in particular relates to an electrochemical method for performing quantitative determination on anthracene content in a water system by taking alizarin red as a probe for modifying a mesoporous material. The material synthesis of a functional modified electrode taking the probe for modifying the mesoporous material is simple and convenient; the modified electrode is simple and convenient to manufacture; the material cost is low; the electrode is easy to update, good in reproducibility and non-toxic, and does not cause environment pollution; and the determining method reduces the interference of coexist substances effectively, and is good in selectivity and high in sensitivity.

The invention belongs to the field of environmental protection and relates to a preparation method of MOFs (metal-organic frameworks) / PVA (polyvinyl alcohol) frozen gel immobilized laccase. The preparation method comprises the steps as follows: (1) synthesizing an MOF; (2) adding quantitative PVA to a pure water solution, and performing heating and stirring to enable PVA to dissolve completely to obtain a PVA solution; (3) adding laccase to water, adding the PVA solution and the MOF after uniform mixing, and removing bubbles by ultrasonic waves after uniformly mixing the mixture in a vortex manner; (4) dropping the mixed solution on a 1*2 cm slide, and applying the solution on the whole slide; (5) placing the slide in a refrigerator at subzero 20 DEG C to be frozen after dropping, taking out the composite, and repeating freezing-thawing cycles to obtain the MOFs / PVA frozen gel immobilized laccase finally. Dye alizarin green is used as a target pollutant, and the prepared MOFs / PVA frozen gel immobilized laccase has obviously increased removal rate for alizarin green in water.

The invention provides a method for the cloning of a pleurotus djamor HP1 laccase gene and the dye decoloration of recombinase. According to the invention, a pair of degenerate primers is designed according to a conserved region of several white-rot fungi laccase gene copper ion binding site amino acids, a laccase cDNA gene core segment is amplified through RT-PCR, and finally a laccase cDNA full-length sequence is amplified by a 3' / 5' RACE technology. The pleurotus djamor cDNA is connected with an expression vector pPICZB to construct a recombinant plasmid pPICZB / lac, and the pichiapastoris SMD1168H is converted by an electric conversion method to obtain an engineering strain. The purified laccase is obtained by DEAE-sepharose CL-6B ion exchange column chromatography, polyethylene glycol concentration and SephadexG-75 gel filtration chromatography. The unique degradation ability of the purified laccase against alizarin red can be applied to the wastewater treatment after dye pollution.

A method for detecting F-content in drinking water includes adding water sample into test tube up to scale, sucking reagent of Zr salt alizarin into dropper and dropping said reagent into water sample test tube according to drip number labeled on reagent bottle of Zr salt alizarin, closing cap of test tube to mix solution in test tube uniformly, comparing sample tube with standard colorimetric plate to seek out corresponding color for obtaining result of F content in water sample.

The high efficiency animal nutrient solution consists of composite amino acids, purple perilla extract solution, alizarin powder, grape seed powder, titanium tetrachloride, manganese sulfate, zinc sulfate, hydrolase, boric acid, calcium gluconate, vitamin B and other components. It is organic chelate prepared through industrial fermentation, extracting amino aids, and other steps. It can promote cell division and proliferation, stimulate enzyme activity, increase cell arranging density, raise animal's immunity and promote animal's growth.

The invention discloses a method for dyeing intermuscular bones of adult megalobrama amblycephala. The method comprises the following steps: step 1, fixing an adult megalobrama amblycephala sample with absolute ethyl alcohol; step 2, rehydrating the adult megalobrama amblycephala sample with the absolute ethyl alcohol and distilled water; step 3, immersing the adult megalobrama amblycephala sample with a TBST solution overnight; step 4, immersing the adult megalobrama amblycephala sample with the distilled water and washing to remove the residual TBST solution; step 5, making the adult megalobrama amblycephala sample transparent with a newly-prepared KOH solution; step 6, transferring the sample to the new KOH solution and dropwise adding an alizarin red dyeing solution to dye until the solution becomes deep purple; step 7, transferring the sample into the new KOH solution and washing off the dyeing solution of the adult megalobrama amblycephala sample; and step 8, transferring the adult megalobrama amblycephala sample to glycerin to be dehydrated and preserved. The method is feasible and an operation process is simple; complicated instruments are not needed; the adult megalobrama amblycephala sample with a transparent body and the intermuscular bones which are dyed into the deep purple color, is obtained; and adhering positions and shapes of the intermuscular bones of the adult megalobrama amblycephala can be clearly observed from the sample.

The invention provides a method for determining fluorine content in gypsum and silica. The method does not require ethanol to eliminate manganese, and uses alizarin S as an indicator, acetic acid-sodium acetate-sodium citrate-sodium chloride as a total ionic strength regulator. A fluoride ion selective electrode method is used to determine low levels of fluorine in gypsum and silica. The method can not only adjust the acidity and the ionic strength of the solution, but also mask the elements such as iron and aluminum in the solution. The method has high sensitivity, fast measurement speed, simple operation and small interference compared with other methods, and has good selectivity, which can provide accurate data.

The invention belongs to the technology of fish marking and releasing, and particularly relates to a method for marking juvenile sciaenidae. The method comprises the steps of firstly conducting configuration of fluorescent dyed mother liquor, dissolving alizarin complexometric indicator powder in water, making the fluorescent dyed mother liquor with the concentration of 0.5-1 g / L, using KOH or NaOH solutions as pH conditioning agents, adjusting the pH value of the fluorescent dyed mother liquor to be 8 to 9, then carrying out marking, diluting the fluorescent dyed mother liquor to be fluorescent dyed liquor with the concentration of 90 ppm to 110 ppm, soaking the juvenile sciaenidae in the fluorescent dyed liquor for 22 hours to 24 hours, and adding pure oxygen to the dyed liquor in the process of soaking. The method for marking the juvenile sciaenidae is easy to operate, after the otolith of the juvenile sciaenidae is dyed, pink is used as the marking color, recognition is easy, poison and damage do not exist, the retention rate of the marking color is high, the retention time is long, the used fluorescence dye can be repeatedly used, and the marking cost is reduced.

The invention relates to the technical field of plant nursery stock cultivation, in particular to a management method for promoting prematurity of potted butterfly orchid. The management method comprises the following steps: removing a butterfly orchid seedling which is 1.5 inches from a pot once every 50 to 52 days, immersing the seedling in a purple non-sulfur germ secreta extracting solution, wrapping the surface of each butterfly orchid leaf with a layer of blue polyethylene film, carrying out treatment with dark light, weak light, moderate light and strong light cyclically in sequence for 4 to 5 cycles, and after the butterfly orchid is bloomed, and watering the root of the butterfly orchid once with a solution with sodium alizarin sulfonate diluted by 800 times every other 5 days. After being treated by the management method, the butterfly orchid seedling which is 1.5 inches can be bloomed in advance within 5 to 6 months, and the flowering phase can be lasted for 60 to 75 days.

The present invention discloses a novel method for model organism zebrafish evaluation of osteoporosis prevention treatment drug effects. The method includes selecting zebrafish as a model animal, grouping and designing experiments by an optimized experimental grouping method, administering by using a preferable drug administration method, using a preferable method to dye zebrafish bones with alizarin red, capturing images by intuitive microscopic observation, and analyzing bone dyed areas to reflect the amount of bone mineralization by using a preferable image analysis software. The entire experimental method is high in operability, can objectively reflect the effects of the drug in zebrafish in vivo on bone mass generation, and can quickly and accurately evaluate the effects of compounds and complex ingredients of traditional Chinese medicine in the prevention and treatment of osteoporosis. The method can overcome the shortcomings that ordinary in-vitro cell experiments are rigor in experimental conditions and difficult to reflect body effect comprehensive results; and overcomes shortcomings that mammal in-vivo experiments are time-consuming, large amount of drug are tested, and labor intensity is high. The method is high in accuracy of experimental results, the experimental method is high in repeatability, the required drug to be tested is relatively less, the cost is low, labor intensity is low, application range is wide, the experimental studies can be conducted in batch, and the working efficiency is high.

A voltammetric pH sensor, especially for characterising wellbore fluids, comprises a plurality of electrodes with a redox active organic compound attached to an electrode and having at least one functional group convertible electrochemically between reduced and oxidized forms with transfer of at least one proton between the compound and surrounding aqueous phase, wherein the compound has at least one substituent group which promotes hydrogen bonding at a said functional group and thereby increases the reaction rate of proton transfer. The substituent group may form an internal hydrogen bond with a redox-convertible group or may enhance polarity to promote electrostatic interaction with water molecules and reduce activation energy. Typical examples include alizarin or 1,2-dihydroxy-anthraquinone (RH=72-48-0), quinizarin or 1,4-dihydroxy-anthraquinone (RN=81-64-1), 2-acetoxy-benzoquinone (RN=1125-55-9), chloranil or 2,3,4,5-tetrachloro-benzoquinone (RN=118-75-2) and 1,4-diamino-2,3-dichloro-anthraquinone (RN=81-42-5) deposited on a glassy carbon electrode. In this way, anomalous measurements at low ionic strength and low concentrations of pH buffering species can be overcome.

The invention discloses an anthraquinone dissolvent dye and preparation and application in dye technical domain, which is characterized by the following: setting the composite as general formula (I); choosing R1 from hydroxyl group or II; choosing R2 from metyl group or ethyl group; adopting vacuum rectifying craft; extracting 2-ethyl group-6-secondary butyl aniline or 2-metyl group-6-secondary butyl aniline; proceeding condensation reaction with 1, 4-alizarin; preparing the general formula (I) component; decreasing the cost of dye. This dye can be used to color oil, hard plastic rubber and resin, which can get brilliance red light blue and bright violet.

The invention discloses a subcutaneous fluorescent labeling method for Sepiella maindroni, comprising the following steps of: (1) anesthesia: selecting Sepiella maindroni to be labeled and placing the Sepiella maindroni into an anesthetic solution MS-222 with the concentration ranging from 40ppm to 70ppm for 0.5 minute to 4 minutes; and (2) labeling: injecting a staining solution into the inner shell of the Sepiella maindroni treated in the step (1) with an injector. Because the invention performs anesthesia treatment on the Sepiella maindroni by using the anesthetic solution MS-222, the Sepiella maindroni basically has no stress when injected, the operation can be conveniently carried out, and the harm to the Sepiella maindroni can be reduced. A special labeling reagent for the subcutaneous fluorescent labeling method for the Sepiella maindroni contains one portion of alizarin complexometric indicator powder and four to eight portions of yoghurt according to weight portion and forms a label in a subcutaneous area which is relatively concentrated, and the label has higher identifiability than a general staining label. Moreover, the method does not need temporary culture for removing floating color after the Sepiella maindroni recovers, and the Sepiella maindroni can be released immediately.

The invention relates to the technical field of aquaculture, in particular to a method for marking stichopus japonicus and detecting the mark. When the stichopus japonicus individual is marked, fasting is performed on the stichopus japonicus for a period of time, then the stichopus japonicus is soaked for 16-48h with alizarin complexometric indicator. When the mark is detected, NaClO water solution is first employed to process stichopus japonicus body wall epidermis or connective tissue marked by the alizarin complexometric indicator, then the stichopus japonicus body wall epidermis or connective tissue are placed under a fluorescence microscope and detected. The marking method is completely performed when the stichopus japonicus normally grows, and no injection or anesthesia is needed to be performed on the stichopus japonicus, the stichopus japonicus living body is not damaged, operation processes for making are reduced and vigor decline of the stichopus japonicus is avoided and mortality rate thereof is reduced. In addition, the employed dye is cheap and easily available, thus saving costs for marking.

The invention relates to a GPR35 receptor agonist and an application thereof as a medicine, and particularly, discloses discovery that an anthraquinone compound from a rubiaceous plant acts on a target site being a GPR35 receptor. The GPR35 receptor agonist herein employs hydroxyl-alizarin and / or one or more than two pharmaceutically acceptable salts of the hydroxyl-alizarin as active components. An in-vitro cell experiment proves that the hydroxyl-alizarin is an agonist of the GPR35 receptor. At present, the study proves that the GPR35 receptor is related to the diseases, comprising asthma, cardiac failure, hypertension, inflammation, coronary heart disease, metabolic syndrome, pain, cancer and the like, and as a result, a new high-effective ligand is provided for the related diseases with definite target sites.

The invention relates to the field of aquatic animals and in particular relates to an aquatic animal marking method and a marking injection agent. 3-aminomethylalizarin-N,N-acetic acid is used for preparing a young inkfish marking reagent, and a 3-aminomethylalizarin-N,N-acetic acid water solution with the content of 250-500ppm and the pH value of 6-10 serves as the injection agent to be injected to the endoskeleton of young inkfish. A dyeing marking method and the reagent have double effects of external marking and internal marking; the dyeing marking method is good in effect, convenient to operate and low in material waste; the death rate of the young inkfish is low; the marking color can be long kept and can be identified by naked eyes.

The invention discloses a pH (potential of hydrogen) value photochromic antibacterial fabric and a preparation method thereof. The method includes the steps: blending combed cotton and silver fibers according to the blending ratio of (92-96):(4-8) to form silver cotton yarns; weaving the silver cotton yarns to form a fabric; pretreating the fabric, drying the fabric by alizarin dye solution or curcumin dye solution and dipping the fabric in pH buffer solution to obtain the pH value photochromic antibacterial fabric. The pH value photochromic antibacterial fabric can determine wound healing degree by observing color change without uncovering a dressing, and injury to a wound is less. Silver components of the fabric exist in a physical elementary substance form, the fabric has better oxidation resistance, and silver ion activity is persistently released, so that persistent antibacterial performances are achieved.

The invention provides application of melatonin in preparation of a medicine for preventing premature senility of mesenchymal stem cells; the mesenchymal stem cells are mesenchymal stem cells obtained from human, pig, mouse or rabbit tissues; influence of the melatonin to the multiplication capacity of premature senility mesenchymal stem cells is detected by using CCK-8; change of the SA-beta-Gal dyeing positive rate after the melatonin acts on the premature senility mesenchymal stem cells is detected and analyzed by using SA-beta-Gal dyeing; change of the cell cycle after the melatonin acts on the premature senility mesenchymal stem cells is detected and analyzed by using a flow cytometry; dyeing is carried out by using calcium nodule alizarin red; and influence of the melatonin to the osteogenesis ability of the premature senility mesenchymal stem cells is quantitatively detected and analyzed by using calcium nodule. Application disclosed by the invention provides a policy for preventing oxidative stress injury through cell therapy based on the mesenchymal stem cells.

The present invention provides a novel alizarin derivative compound and a simplified and low cost method for preparing an alizarin derivative compound including: obtaining a compound represented by Formula (2) using a compound represented by Formula (3); and obtaining an alizarin derivative compound represented by Formula (1) using the compound represented by Formula (2); in Formulae (1) to (3), R1 represents a hydrogen atom or a substituent; n represents an integer of 1 to 3, L represents a specific alkyl group; Q represents an atomic group needed to form an aromatic ring or a heteroaromatic ring with adjacent carbon atoms; and P represents an atomic group which includes an atom(s) selected from a hydrogen atom, a carbon atom, an oxygen atom, a sulfur atom, a silicon atom and a boron atom, and which is needed to form a ring structure group with adjacent two oxygen atoms and two carbon atoms;

The invention discloses a method for detecting sesame oil adulteration, and relates to the technical field of sesame oil quality monitoring. The method specifically comprises the following steps: adding standard sesame oil into a mixed system prepared by mixing polyphenol dyes and zinc ions for reaction, and recording color change as a standard color; adding a to-be-detected sesame oil sample into a mixed system prepared by mixing polyphenol dyes and zinc ions for reaction, recording color change, and comparing the color change with the standard color; and judging whether the sesame oil is adulterated according to the color difference. According to the invention, based on the coordination effect between Zn<2+> and compounds in the sesame oil, the weak complexing effect between Zn<2+> and polyphenol dyes (alizarin red S, bromo-pyrophenol red, pyrogallol red and catechol purple) can be destroyed, and the color recovery of the polyphenol dyes is realized; and the method is of great significance in protecting health and benefits of consumers, detecting and identifying related departments and maintaining a good market environment.

The invention discloses a method for rapidly identifying vegetable dyes in dyed textile. According to the method, whether a test sample contains specific representative components in plant dyes or notis determined with HPLC (high performance liquid chromatography), and whether the test sample is dyed with corresponding plant dyes is identified. The plant dyes comprise at least one of madder, plant indigo blue, gardenia yellow and turmeric, and the specific representative components in the plant dyes specifically comprise alizarin and / or purpurin in the madder, curcumin in the turmeric, indirubin in the plant indigo blue and crocetin in the gardenia yellow. The same stripping solvent and sample preparation method is used, alizarin, purpurin, curcumin, indirubin and crocetin are determinedsimultaneously with HPLC, finally, the four plant dyes including the madder, the plant indigo blue, the gardenia yellow and the turmeric in dyed textile can be rapidly identified, and the method is rapid, accurate and efficient.