142 results about "Alizarin" patented technology

The invention provides an flow battery negative electrode electrolyte based on alizarin or derivatives thereof, and a flow battery adopting the electrolyte as a negative electrode. The electrolyte is an alkaline aqueous solution containing the alizarin or the derivatives thereof. The alizarin and the derivatives contain at least one of alizarin (1,2-dihydroxyanthraquinone), alizarin red (1,2-dihydroxyanthraquinone-3-sulfonic acid) or alizarin fluorin blue (3-alizarin methylamine-N,N-diacetic acid and mixtures thereof. An alkali used in the invention comprises at least one of sodium hydroxide, potassium hydroxide and a sodium hydroxide and potassium hydroxide mixture. The working temperature of the electrolyte is 10-50 DEG C; and the flow battery based on the electrolyte is a traditional structure, and comprises a positive electrode liquid storage tank 1, a positive electrode electrolyte 3, a negative electrode liquid storage tank, the negative electrode electrolyte 4, pumps 7 and 8, and a cation exchange membrane 9 for conducting cations. The active substance of the alizarin flow battery negative electrode electrolyte is bulk anions, and difficultly penetrates through a proton exchange membrane; and the electrolyte disclosed in the invention has the characteristics of no toxicity, no pollution, no rare earth elements, and obvious price advantage.

The invention relates to a method for marking gold sepia, wherein the invention is characterized in that: cultivating the hatched sepia for 5-20 days, dissolving coloring agent into fresh water, adding the water solution into the container with sepia and seawater, to make its coloring agent content as 60-80ppm; emerging and coloring for 20-28hours; said coloring agent is alizarin complex instructor. The invention has simple process without toxic and hurt, while it can color many types of sepia at one time.

The invention discloses a method for detecting amoxicillin residual quantity in milk and meat products. The method includes the following steps: preparing amoxicillin standard aqueous solution; adding a nonionic surfactant; reacting the obtained solution with quinone or alizarin to get a charge transfer system; determining the wavelength scanning range of fluorescence or ultraviolet; further calculating the linear range, working curve and other parameters of the standard solution; processing the sample to be detected by corresponding charge transfer reaction; determining reaction parameters under ultraviolet or fluorescence; and calculating to obtain the amoxicillin residue quantity. The technology further enhances the value of fluorescence intensity or UV absorption by utilizing the sensitizing effect of non-ionic surfactants, thus meets the low-residue testing requirement; simultaneously improves the system stability significantly, makes up the disadvantage of poor detection stability of fluorescence in the earlier application, and is more feasible in the actual detection.

The invention belongs to the field of environmental protection and relates to a preparation method of MOFs (metal-organic frameworks)/PVA (polyvinyl alcohol) frozen gel immobilized laccase. The preparation method comprises the steps as follows: (1) synthesizing an MOF; (2) adding quantitative PVA to a pure water solution, and performing heating and stirring to enable PVA to dissolve completely to obtain a PVA solution; (3) adding laccase to water, adding the PVA solution and the MOF after uniform mixing, and removing bubbles by ultrasonic waves after uniformly mixing the mixture in a vortex manner; (4) dropping the mixed solution on a 1*2 cm slide, and applying the solution on the whole slide; (5) placing the slide in a refrigerator at subzero 20 DEG C to be frozen after dropping, taking out the composite, and repeating freezing-thawing cycles to obtain the MOFs/PVA frozen gel immobilized laccase finally. Dye alizarin green is used as a target pollutant, and the prepared MOFs/PVA frozen gel immobilized laccase has obviously increased removal rate for alizarin green in water.

The invention provides a method for the cloning of a pleurotus djamor HP1 laccase gene and the dye decoloration of recombinase. According to the invention, a pair of degenerate primers is designed according to a conserved region of several white-rot fungi laccase gene copper ion binding site amino acids, a laccase cDNA gene core segment is amplified through RT-PCR, and finally a laccase cDNA full-length sequence is amplified by a 3'/5' RACE technology. The pleurotus djamor cDNA is connected with an expression vector pPICZB to construct a recombinant plasmid pPICZB/lac, and the pichiapastoris SMD1168H is converted by an electric conversion method to obtain an engineering strain. The purified laccase is obtained by DEAE-sepharose CL-6B ion exchange column chromatography, polyethylene glycol concentration and SephadexG-75 gel filtration chromatography. The unique degradation ability of the purified laccase against alizarin red can be applied to the wastewater treatment after dye pollution.

A method for detecting F-content in drinking water includes adding water sample into test tube up to scale, sucking reagent of Zr salt alizarin into dropper and dropping said reagent into water sample test tube according to drip number labeled on reagent bottle of Zr salt alizarin, closing cap of test tube to mix solution in test tube uniformly, comparing sample tube with standard colorimetric plate to seek out corresponding color for obtaining result of F content in water sample.

The high efficiency animal nutrient solution consists of composite amino acids, purple perilla extract solution, alizarin powder, grape seed powder, titanium tetrachloride, manganese sulfate, zinc sulfate, hydrolase, boric acid, calcium gluconate, vitamin B and other components. It is organic chelate prepared through industrial fermentation, extracting amino aids, and other steps. It can promote cell division and proliferation, stimulate enzyme activity, increase cell arranging density, raise animal's immunity and promote animal's growth.

The invention discloses a method for dyeing intermuscular bones of adult megalobrama amblycephala. The method comprises the following steps: step 1, fixing an adult megalobrama amblycephala sample with absolute ethyl alcohol; step 2, rehydrating the adult megalobrama amblycephala sample with the absolute ethyl alcohol and distilled water; step 3, immersing the adult megalobrama amblycephala sample with a TBST solution overnight; step 4, immersing the adult megalobrama amblycephala sample with the distilled water and washing to remove the residual TBST solution; step 5, making the adult megalobrama amblycephala sample transparent with a newly-prepared KOH solution; step 6, transferring the sample to the new KOH solution and dropwise adding an alizarin red dyeing solution to dye until the solution becomes deep purple; step 7, transferring the sample into the new KOH solution and washing off the dyeing solution of the adult megalobrama amblycephala sample; and step 8, transferring the adult megalobrama amblycephala sample to glycerin to be dehydrated and preserved. The method is feasible and an operation process is simple; complicated instruments are not needed; the adult megalobrama amblycephala sample with a transparent body and the intermuscular bones which are dyed into the deep purple color, is obtained; and adhering positions and shapes of the intermuscular bones of the adult megalobrama amblycephala can be clearly observed from the sample.

The invention provides a method for determining fluorine content in gypsum and silica. The method does not require ethanol to eliminate manganese, and uses alizarin S as an indicator, acetic acid-sodium acetate-sodium citrate-sodium chloride as a total ionic strength regulator. A fluoride ion selective electrode method is used to determine low levels of fluorine in gypsum and silica. The method can not only adjust the acidity and the ionic strength of the solution, but also mask the elements such as iron and aluminum in the solution. The method has high sensitivity, fast measurement speed, simple operation and small interference compared with other methods, and has good selectivity, which can provide accurate data.

The present invention discloses a novel method for model organism zebrafish evaluation of osteoporosis prevention treatment drug effects. The method includes selecting zebrafish as a model animal, grouping and designing experiments by an optimized experimental grouping method, administering by using a preferable drug administration method, using a preferable method to dye zebrafish bones with alizarin red, capturing images by intuitive microscopic observation, and analyzing bone dyed areas to reflect the amount of bone mineralization by using a preferable image analysis software. The entire experimental method is high in operability, can objectively reflect the effects of the drug in zebrafish in vivo on bone mass generation, and can quickly and accurately evaluate the effects of compounds and complex ingredients of traditional Chinese medicine in the prevention and treatment of osteoporosis. The method can overcome the shortcomings that ordinary in-vitro cell experiments are rigor in experimental conditions and difficult to reflect body effect comprehensive results; and overcomes shortcomings that mammal in-vivo experiments are time-consuming, large amount of drug are tested, and labor intensity is high. The method is high in accuracy of experimental results, the experimental method is high in repeatability, the required drug to be tested is relatively less, the cost is low, labor intensity is low, application range is wide, the experimental studies can be conducted in batch, and the working efficiency is high.

The invention discloses a subcutaneous fluorescent labeling method for Sepiella maindroni, comprising the following steps of: (1) anesthesia: selecting Sepiella maindroni to be labeled and placing the Sepiella maindroni into an anesthetic solution MS-222 with the concentration ranging from 40ppm to 70ppm for 0.5 minute to 4 minutes; and (2) labeling: injecting a staining solution into the inner shell of the Sepiella maindroni treated in the step (1) with an injector. Because the invention performs anesthesia treatment on the Sepiella maindroni by using the anesthetic solution MS-222, the Sepiella maindroni basically has no stress when injected, the operation can be conveniently carried out, and the harm to the Sepiella maindroni can be reduced. A special labeling reagent for the subcutaneous fluorescent labeling method for the Sepiella maindroni contains one portion of alizarin complexometric indicator powder and four to eight portions of yoghurt according to weight portion and forms a label in a subcutaneous area which is relatively concentrated, and the label has higher identifiability than a general staining label. Moreover, the method does not need temporary culture for removing floating color after the Sepiella maindroni recovers, and the Sepiella maindroni can be released immediately.

The invention relates to the technical field of aquaculture, in particular to a method for marking stichopus japonicus and detecting the mark. When the stichopus japonicus individual is marked, fasting is performed on the stichopus japonicus for a period of time, then the stichopus japonicus is soaked for 16-48h with alizarin complexometric indicator. When the mark is detected, NaClO water solution is first employed to process stichopus japonicus body wall epidermis or connective tissue marked by the alizarin complexometric indicator, then the stichopus japonicus body wall epidermis or connective tissue are placed under a fluorescence microscope and detected. The marking method is completely performed when the stichopus japonicus normally grows, and no injection or anesthesia is needed to be performed on the stichopus japonicus, the stichopus japonicus living body is not damaged, operation processes for making are reduced and vigor decline of the stichopus japonicus is avoided and mortality rate thereof is reduced. In addition, the employed dye is cheap and easily available, thus saving costs for marking.

The invention provides application of melatonin in preparation of a medicine for preventing premature senility of mesenchymal stem cells; the mesenchymal stem cells are mesenchymal stem cells obtained from human, pig, mouse or rabbit tissues; influence of the melatonin to the multiplication capacity of premature senility mesenchymal stem cells is detected by using CCK-8; change of the SA-beta-Gal dyeing positive rate after the melatonin acts on the premature senility mesenchymal stem cells is detected and analyzed by using SA-beta-Gal dyeing; change of the cell cycle after the melatonin acts on the premature senility mesenchymal stem cells is detected and analyzed by using a flow cytometry; dyeing is carried out by using calcium nodule alizarin red; and influence of the melatonin to the osteogenesis ability of the premature senility mesenchymal stem cells is quantitatively detected and analyzed by using calcium nodule. Application disclosed by the invention provides a policy for preventing oxidative stress injury through cell therapy based on the mesenchymal stem cells.

The present invention provides a novel alizarin derivative compound and a simplified and low cost method for preparing an alizarin derivative compound including: obtaining a compound represented by Formula (2) using a compound represented by Formula (3); and obtaining an alizarin derivative compound represented by Formula (1) using the compound represented by Formula (2); in Formulae (1) to (3), R¹ represents a hydrogen atom or a substituent; n represents an integer of 1 to 3, L represents a specific alkyl group; Q represents an atomic group needed to form an aromatic ring or a heteroaromatic ring with adjacent carbon atoms; and P represents an atomic group which includes an atom(s) selected from a hydrogen atom, a carbon atom, an oxygen atom, a sulfur atom, a silicon atom and a boron atom, and which is needed to form a ring structure group with adjacent two oxygen atoms and two carbon atoms;

The invention belongs to the field of constructional engineering and relates to an indicator for determining alkalinity of the finished product of cement and application thereof. The indicator is prepared and finished by the following steps of: (1) dissolving phenolphthalein into 60% ethanol according to the massic volume ratio of 1:1000; (2) dissolving thymolphthalein into 90% ethanol according to the massic volume ratio of 1:100; (3) dissolving alizarin red into 90% ethanol according to the massic volume ratio of 4:1000; (4) dissolving titan yellow into 90% ethanol according to the massic volume ratio of 3:1000; and (5) fully mixing the solution of the same volume obtained in the previous four steps, so as to prepare the indicator for determining the alkalinity of the finished product of the cement. The indicator has the advantages of simpleness in raw material, extremely low cost, convenience and practicability.

The invention discloses a method for rapidly identifying vegetable dyes in dyed textile. According to the method, whether a test sample contains specific representative components in plant dyes or notis determined with HPLC (high performance liquid chromatography), and whether the test sample is dyed with corresponding plant dyes is identified. The plant dyes comprise at least one of madder, plant indigo blue, gardenia yellow and turmeric, and the specific representative components in the plant dyes specifically comprise alizarin and/or purpurin in the madder, curcumin in the turmeric, indirubin in the plant indigo blue and crocetin in the gardenia yellow. The same stripping solvent and sample preparation method is used, alizarin, purpurin, curcumin, indirubin and crocetin are determinedsimultaneously with HPLC, finally, the four plant dyes including the madder, the plant indigo blue, the gardenia yellow and the turmeric in dyed textile can be rapidly identified, and the method is rapid, accurate and efficient.

The invention discloses a fluorinion (F<->) colorimetric sensor and a preparation method thereof, belonging to the technical field of preparation of colorimetric sensors. The F<-> colorimetric sensor is made of an organic-inorganic composite material. A composite film is prepared from nano hydrotalcite colloid particles with positive electricity and sodium salt guest molecules of an alizarin complexing reagent (AC) with negative electricity under the action force of electrostatic attraction by a supermolecule layer-by-layer assembling method; quick (1s) ultraviolet response of a film sensor to the F<-> with different concentrations is investigated; and desorption of the film sensor to the F<-> is realized by competitive combination of F<-> and Al<3+>, so that repeated detection of the sensor to the F<-> is further realized. A hydrotalcite laminate of the prepared F<-> colorimetric sensor is a microcosmic rigid environment, so the guest AC molecules can be dispersed to the maximum degree, the aggregation of the guest AC molecules can be effectively prevented, and therefore the optical stability and the heat stability of the AC molecules are maintained. The sensor has good selectivity to the F<-> and shows a linear relationship in a certain range, and the color change can be observed by naked eyes.