The invention belongs to the technical field of fish
ecology, and particularly relates to a nude
carp otolith embedding method based on a longitudinal section and a preparation method and application of an
otolith grinding sheet. The
epoxy resin and the curing agent are mixed according to a certain
mass ratio and then placed on the vegetable parchment to embed the longitudinal section of the naked
carp otolith, the vegetable parchment is resistant to high temperature and high in strength and is not prone to deformation caused by high temperature, and the cured embedding piece is easily separated from the vegetable parchment; the
embedding agent is low in
shrinkage rate after being cured, the otolith is not prone to fracture, the
hardness requirement of subsequent
grinding can be met, adhesion is high, degumming is avoided in the subsequent
grinding process, and fragmentation of the naked
carp otolith is effectively avoided. According to the method, the embedding piece polished for the first time is immediately immersed in water, oxidation of the polished surface of the otolith can be prevented, subsequent
polishing is continued, and the otolith grinding piece with clear wheel lines is obtained. The method is simple in operation process, does not need to use a high-cost and low-efficiency embedding mold, reduces the preparation cost, and improves the preparation efficiency.