Fish branchiostegite marking method

A technique for marking, fish, applied in the field of fish farming

Inactive Publication Date: 2013-07-03
YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Otolith fluorescent labeling method has great limitations in the marking and release of fish, especially rare fish, due to the disadvantage that the fish must be killed to identify the mark.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Use water with a pH value of 6.5, a water temperature of 5°C, and a dissolved oxygen of 5 mg / L as a solvent, dissolve 50 mg of alizarin red in every liter of water, and prepare an aqueous solution of alizarin red with a concentration of 50 mg / L;

[0022] 2. Put the prepared alizarin red aqueous solution into the container, and use the air pump to inflate the solution in the container for 6 hours;

[0023] 3. Put 50 crucian carp to be marked (weight 100-296g) into the alizarin red aqueous solution and soak for 18 hours. At this time, the depth of the solution is 20cm higher than that of the crucian carp. Inflatable;

[0024] 4. After the marking, put the 50 crucian carp that have been marked into the running water fish pond for 3 months and then take out the crucian carp. A red spot mark with a diameter of 0.2 cm can be observed on the gill cover of the crucian carp with the naked eye.

Embodiment 2

[0026] 1. Use water with a pH value of 6.8, a water temperature of 10°C, and a dissolved oxygen of 7 mg / L as a solvent, dissolve 100 mg of alizarin red in every liter of water, and prepare an aqueous solution of alizarin red with a concentration of 100 mg / L;

[0027] 2. Put the prepared alizarin red aqueous solution into the container, and use the air pump to inflate the solution in the container for 6 hours;

[0028] 3. Soak 50 crucian carp (weight 131-269g) to be marked in the alizarin red aqueous solution for 14 hours. At this time, the depth of the solution is 35cm higher than that of the crucian carp. Inflatable;

[0029] 4. After the marking, put the marked 50 crucian carp into the running water fish pond for 3 months and then take out the crucian carp. A red spot mark with a diameter of 0.6 cm can be observed on the gill cover of the crucian carp with the naked eye.

Embodiment 3

[0031] 1. Use water with a pH value of 7.5, a water temperature of 25°C, and a dissolved oxygen of 8 mg / L as a solvent, dissolve 200 mg of alizarin red in each liter of water, and prepare an aqueous solution of alizarin red with a concentration of 200 mg / L;

[0032] 2. Put the prepared alizarin red aqueous solution into the container, and use the air pump to inflate the solution in the container for 6 hours;

[0033] 3. Soak 50 crucian carp (weight 127-283g) to be marked in the alizarin red aqueous solution for 12 hours. At this time, the depth of the solution is 50cm higher than that of the crucian carp. Inflatable;

[0034] 4. After the marking, put the marked 50 crucian carp into the running water fish pond for 3 months and then take out the crucian carp. A red spot mark with a diameter of 1.0 cm can be observed on the gill cover of the crucian carp with the naked eye.

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PUM

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Abstract

The invention provides a fish branchiostegite marking method. The method comprises the following steps of: 1, selecting water with a pH value of 6.5-7.5, the temperature of 5-25 DEG C and the dissolved oxygen volume of 5-9mg/L as a solvent, and dissolving alizarin red into the solvent so as to prepare an alizarin red aqueous solution with the concentration of 50-200mg/L; 2, after air is inflated in the solution for 6 hours by using an inflator pump, putting fishes to be marked and soaking, and in the process of soaking, always carrying out air inflation operation; 3, enabling the depth of a soaking solution to be 20-50cm higher than the heights of the soaked fishes; 4, carrying out soaking for 12-18 hours, and enabling the soaking time to be inversely proportional to the concentration of the solution; and 5, putting marked fishes into a continuous-flow fishpond to culture for 3 months, wherein red spotted marks with the diameter of 0.2-1.0cm occur at fish branchiostegite. According to the method, the problems that an in-vitro marking method, otolith fluorescent marking and temperature marking and the like cause large damages on fishes, and the mark loss ratio is high are avoided.

Description

technical field [0001] The invention relates to the field of fish farming, in particular to a method for marking fish gill covers. Background technique [0002] The gill cover of fish is a calcified tissue that is exposed on the surface of the fish body. During the material exchange between fish and the external environment, chemical elements in the environment are continuously ingested by fish, and after a series of metabolism and circulation in the body, some elements will eventually be deposited on the gill cover. Therefore, by changing the growth environment of fish, specific marks can be formed on the gill cover of fish to distinguish it from natural populations. [0003] The mark-recapture method is usually used to evaluate the effect of fish proliferation and release, which requires marking the fish before releasing to distinguish the released group from the wild group. At present, fish marking methods include in vitro labeling method, in vivo labeling method, bioma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 杜浩梁志强危起伟李罗新李雷杨晓鸽谢晓
Owner YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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