The application discloses PDRN and a preparation method and application thereof, and the preparation method of the PDRN comprises the following steps: performing
fermentation treatment by using
lactobacillus plantarum, collecting bacterial bodies after
centrifugation of obtained
fermentation culture solution; resuspending the obtained bacterial bodies, homogenizing and crushing, centrifuging and removing the precipitate, concentrating by using a 5kDa
hollow fiber membrane, purifying the obtained concentrated solution by using a Q column, and then performing concentration and liquid replacement, so that high-purity PDRN is obtained. The PDRN is obtained by
fermentation of
lactobacillus plantarum for the first time, and compared with the PDRN from current salmon or salmon
trout, the production cost of the PDRN is lower, so that a new way is provided for production of the PDRN. Through experimental
verification, the PDRN obtained by the method has higher activity in promoting
cell proliferation and promoting collagen production.