407 results about "Fish egg" patented technology

The invention belongs to the field of fish molecular breeding, and particularly relates to a fish breeding method for improving CRISPR-Cas9 gene editing and passage efficiency through fish roe preserving fluid. According to the method, the fish roe preserving fluid technology, the microinjection technology an the CRISPR-Cas9 gene editing technology are ingeniously combined, the targeting efficiency of the CRISPR-Cas9 gene editing technology and gene editing passage efficiency are greatly improved, the screening time needed by a gene editing method for fish breeding is obviously saved, and the method is of great significance in promoting rapid development of gene editing fish breeding.

A fish allogenous frozen-sperm induced gynogenesis method comprises a frozen storage method of the allogenous fish sperm, a genetic inactivation method, an induced method for the gynogenesis diploid fry and an identification method for the gynogenesis fry. The present invention establishes the method adopting the frozen-sperm of the sea bass to induce the egg gynogenesis of a plurality of sea fishes for the first time, which selects the cold-shock starting time, cold-shock temperature and the cold-shock durance time that are separately suitable for fish eggs of the strip-spotted flounder, the turbot and the canine-tooth paralichthys olivaceus to establish a micro-satellite marking identification method of the gynogenesis fry. The percentage rate adopting the present invention to acquire the gynogenesis fry of the strip-spotted flounder, the turbot and the canine-tooth paralichthys olivaceus is 7.8 percentages to 40.7 percentages, 26 percentages to 34 percentages and 10 percentages to 25 percentages respectively. The established method of the present invention is characterized in advancing, high efficiency, accuracy and reliability, which has an important application value in fish gender controlling and the production of the all-female fries and has a broad popularization and application prospect on fish cultivation and fish breeding.

A method for preparing paraffin section of seawater roe includes selection of fixing agent, confirmation of dewatering process, confirmation of transparent process, confirmation of wax penetration process, improvement of dyeing mode. In the method, egg capsule stripping process being difficultly operated is omitted.

The invention relates to an artificial reproduction method for schizothorax wangchiachii. The method mainly comprises the following seven steps: bait preparation, parent fish sourcing, parent fish domestication, parent fish selection, artificial insemination, fish egg incubation and fish fry feeding. The artificial reproduction method for schizothorax wangchiachii has the beneficial effects that oxytocin is not used, the schizothorax wangchiachii can lay eggs and produce sperms, and relatively high fertility rate and hatchability can be obtained; the problem that female and male wild fish do not develop or develop asynchronously in a pond culture environment is solved fundamentally; the damage on the parent fish of the schizothorax wangchiachii is reduced, and the cultivation cost is also reduced; the method is reasonable and efficient, the hatchability is improved, and the waste of fish egg resources is reduced; and the aberration rate of the fish fry is reduced while the survival rate of the fish fry is improved.

The present invention relates to microscopic tissue section technology, and establishes one effective tissue section method for the fertilized egg of sea water left eyed founder and right-eyed founder based on the features of the fertilized egg of sea water left eyed founder and right-eyed founder. The fertilized egg is polylecithal pelagic egg with high egg membrane toughness and narrow perivitelline space. The tissue section method includes the following steps: 1. fixing the polylecithal pelagic egg with Bouiní»s fixing fluid and optimizing treatment period; 2. pricking the egg membrane with dissecting needle in the vegetative pole direction to peel off the egg membrane; and 3. directly embedded to fix embryo with hot agar. The method is simple and effective, and may be also applied in the tissue section of other sea water fish egg.

The invention provides a cultivation method for purifying water and increasing the growth rate of fish fries, fish fingerling and adult fishes, and a six-level water processing method. By utilizing the appropriate ecological microorganism, the functions of regulating the water quality, purifying the water source and providing fish bait are played in the important period of different stages for the fish incubation, the fish fry growth, the fish fingerling cultivation and the adult fish growth. The fish growth rate is increased, the probability of the fish disease occurring is reduced, and therefore the production efficiency for the fish cultivation is improved.

It is an object of the invention to provide a method for processing a very large number of fish eggs for gene injection and so on, which employs a simple system with high performance and a reasonable construction expense. It is another object to provide the method having an water tank apparatus capable of producing a large number of fish eggs continuously.A large number of water tank groups having an independent drainage passage each is lighted up in turn with a predetermined interval. An egg-collecting case with a net bottom is set in the drainage passage connecting to the water tank group to which the lighting is started. The water tank group consists of a plurality of tank unit accommodating fishes each. Each book-shaped tank unit has a rectangular-shaped upper portion and a cone-shaped lower portion. Rotating water stream is formed in each tank unit.

The invention relates to an artificial propagation method of yellow catfish fry, which is characterized in that: sexually mature yellow catfish over three years are chosen as the parent fishes; the female and male ratio is 3:1; a plurality of barrels are arranged in a spawning pond as fish nests; parent fish density in the spawning pond is 1 to 2 groups / m2; each group of parent fishes uses a barrel; the sand with a thickness ranging form 1cm to 5cm is arranged at the bottom of the barrel for the debonding of fish eggs; in spawning season, exogenous hormone is injected to the basal behind the dorsal fins of the parent fishes to induce ovulation and spermiation; an inversed conical container with smooth inner wall is used as the hatching barrel; fertilized eggs are arranged in the hatching barrel for hatching in flowing water; the dissolved oxygen in the water of the hatching barrel is larger than 5.5 mg / L; when hatching is over, fry is arranged in a cement pool for temporary rearing for 40 to 70 hours, then the fry is packaged in plastic bags with water and oxygen. The artificial propagation method of yellow catfish fry has the advantages that, well developed yellow catfish fry can be acquired; the operation is simple; the utilization rate of parent fishes is high; scale production of yellow catfish fry can be realized, and the invention has significant meaning for the intensive culture of yellow catfish.

The invention provides a portable fish egg incubator, comprising an incubation barrel, a micro magnification device, a tripod, a water inlet tube, an inlet pipe and an overflow pipe, wherein the incubation barrel is internally provided with a temperature regulator and a short-wave ultraviolet generator; the lower part of the incubation barrel is provided with a circular hole structure; the incubation barrel is stored with water for incubation; the short-wave ultraviolet generator sterilizes water for incubation; the micro magnification device amplifies the fish eggs in the incubation barrel; the tripod is provided with a wheel to fix or move the incubator; the water inlet pipe, the inlet pipe and the overflow pipe are communicated with each other and are communicated with the incubation barrel through the water inlet; the water inlet at the bottom of the incubation barrel is provided with an air inlet and holds the fish eggs in an all-dimensional manner by utilizing flow current and airflow for incubation. The invention has simple structure, is convenient for moving and for tracking to observe embryonic development; the short-wave ultraviolet generating device can prevent the breeding of water molds, improve incubation rate of fish eggs; the overflow pipe can control water level. Therefore, no overflow tank is required to be added and the cost is saved. The invention has strong controllability, demersal eggs, viscid eggs and pelagic eggs can be incubated directly.

The invention relates to a breeding method for parent fishes, particularly to a breeding method for goldfishes. The breeding method comprises the following five stages: (1) managing of the parent fish breeding environment; (2) selecting of parent fish seedlings; (3) managing of parent fishes awaiting delivery; (4) artificial hatching of fish eggs; (5) breeding of fish seedlings. By stagedbreeding of the fish seedlings and reasonablymanaging of the parent fish breeding environment, female parent fishes easy to be fertilized and to lay fish eggs are selected; in combination with artificial fertilization, female parent fishes are guaranteed to be high in fertilization rate and pure in fish seedlings; by adopting fish nests to hatch fish eggs, the developmental state of fish eggs is easy to observe, breeding is advanced, the survival rate of fish eggs is improved, the development temperature of fish eggs is easy to control in the hatching period, the fish nests are fast in temperature rising, water consumption is less, whether fertilized eggs are good or not is easy to distinguish, the hatching rate of goldfishes is greatly improved, and the yield of seedlings is improved.

The invention relates to a hatching method for adhesive fish eggs, belonging to the technical field of fish reproduction. The invention is an improved invention. The fish nests of the invention are flat hatching net sheets sewn by using a frame made of iron wires and polyethylene net sheets. On each hatching net sheet 7000-8000 eggs with grain size of 2mm are evenly distributed. The hatching net sheets on which eggs are distributed are statically placed for two minutes and then are sequentially suspended in a covered hatching pond for hatching in running water. Fifteen to twenty hatching net sheets are placed in each square meter of water surface in the hatching pond. Hatching water flow is controlled to be 3-5m<3> / hour, water temperature is controlled to be 11 DEG C-18 DEG C and daily temperature difference does not exceed 2 DEG C; broken egg skins and other impurities are absorbed out everyday through siphonage; the date that fish fries break through egg membranes is the seventh day to the fifteenth day. The invention has the advantages that the hatching facilities are simple, the operation is convenient, the water flow exchange is good, the damaged eggs and the dead fries can be observed easily and removed in time, the water molds are effectively prevented from breeding, the hatching rate of fertilized eggs is high and the promotion and the popularization are easy.

The invention provides a method for producing instant large yellow croaker roes, which comprises the steps of raw material selection-removal of fishy smell-stewing-grinding and sieving-frying-antioxidation processing-forming-drying-cooling-packing-finishing. The method is simple in process and low in production cost; and for the produced instant croaker roes, the fishy smell is removed, and the nutrition and the unique mouthfeeling of the roes are remained, therefore, the instant large yellow croaker roe is a new generation of low-heat healthy leisure food.

The invention discloses a drifting roe hatching device. The drifting roe hatching device comprises a cylindrical barrel and a contraction barrel located below the cylindrical barrel, wherein the top of the contraction barrel communicates with the cylindrical barrel; the bottom area of the contraction barrel is smaller than the top area of the contraction barrel; and the cylindrical barrel is composed of an upper edge fixing ring, a lower edge fixing ring and a first annular gauze surrounding the two fixing rings. The contraction barrel is composed of a contraction fixing ring located below thecontraction cylinder and a second annular gauze for surrounding the contraction fixing ring and the lower edge fixing ring; and the diameter of the contraction fixing ring is smaller than that of thelower edge fixing ring. By the adoption of the drifting roe hatching method and hatching device, the flushing flow can be adjusted according to different drifting roes, so that the optimal effect isachieved, and the survival rate of the roes is increased; and the hatching device is simple in manufacturing process, low in manufacturing cost and convenient to carry.

The invention discloses a three-dimensional artificial fish nest with a floating frame for adhesive eggs. The three-dimensional artificial fish nest is characterized by comprising fixed piles and the floating frame, wherein the floating frame is fixed on the fixed piles via fixing cables, a plurality of cables suspended with sinkers are arranged below the floating frame, and a plurality of adhesion matrixes are uniformly bound on each cable. The three-dimensional artificial fish nest disclosed by the invention can imitate the roots of aquatic plants or trees needing to be well adhered by fertilized eggs, and provide an omni-directional and three-dimensional type batch development place for the fertilized eggs of fishes laying adhesive eggs. The three-dimensional artificial fish nest is simple in structure, as well as convenient to throw and detach; a floating-type structure is not easy to be influenced by the change of a water surface; the adhesion area of the fish eggs is larger, and the three-dimensional space of a water body is adequately utilized; and large-scale propagation conditions can be provided for artificial breeding for fishes laying adhesive eggs living in different water layers and resource enhancement for natural populations. The three-dimensional artificial fish nest is especially suitable for artificial breeding for fishes living in the middle layer and the low layer of the water body.

The invention provides an artificial inseminating and hatching method of high-viscosity fish eggs, capable of solving the problems of the lower inseminating rate and hatching rate caused by multi-layer fish eggs in the prior art. Due to the viscosity of the high-viscosity fish eggs, the high-viscosity fish eggs are inseminated after being shaped to be a flat-layer fish eggs piece which is arranged by means of a single layer and is compactly arranged by a series of artificial methods, so that the fish eggs can not be agglomerate, and the fish eggs inseminating rate is improved since the fish eggs can be abundantly contacted with the sperm when the fish eggs are inseminated; and the flat-layer fish eggs piece which is artificially shaped and compactly arranged by means of the single layer is putted into a hatching net cage to be hatched in an oxygenating way, so that the abundant dissolved oxygen can be obtained when the oosperm is hatched, and the hatching rate of the oosperm is greatly improved.

The invention relates to an artificial propagation and roe incubation method for freshwater fishes, in particular to an artificial propagation and roe incubation method for schizopygopsis malacanthus, and belongs to the technical field of artificial propagation and roe incubation of fishes. The artificial propagation and roe incubation method for schizopygopsis malacanthus mainly comprises the steps of parent fish selection, spawning induction, artificial impregnation, roe incubation and fry raising. The artificial propagation and roe incubation method for schizopygopsis malacanthus has the advantages that the fertilization rate can reach 89.6%, the incubation rate can reach 95%, and the problem of resource scarcity caused by environmental problems is resolved; the fry incubation process is convenient to conduct, the controllability is improved, and requirements for manpower and management in the incubation process are lowered.

A suspended fish egg incubator is composed of a net incubation bucket and a suspended bracket which is used for hanging the net incubation bucket and suspensible on the water face, and an air cascade head is arranged at the bottom of the net incubation bucket. The invention has the beneficial effects that: the structure is simple and the manufacture cost is low; a micro air pump is used for replacing a water tower to supply water for stirring the fish eggs being incubated, the mounting and using are more convenient and the cost is low, and the water source pollution influence can be avoided; mounting and dismounting and carrying are convenient, and the using is not restrained by places; the invention is applicable to direct fish egg incubation in mountain reservoir, lakes and other natural waters as well as in indoor pools, the water temperature can be adjusted, the incubation period can be shortened, and the incubation rate can be improved; use and management are convenient and the labor intensity is light; the invention provides a simple, convenient, efficient and economic fish egg incubation device for the small-scale proliferation of young fishes and famous-brand high-quality marine young products.

The invention discloses an ecology-imitating cultivating breeding method for parent fishes of scaphesthes macrolepis. The method specifically comprises the steps of parent-fish ecologic strengthening cultivating, mature parent-fish selecting, artificial inseminating, germ cell incubating and the like. By means of an artificial ecologic technologic mean, specific nutrition bait material is prepared in a matched mode, ecological factor and nutrition requirements for gonad development of the scaphesthes macrolepis are met so that gonads of the parent fishes mature naturally, any synthetic auxins are not used in a whole breeding process, mature parent fishes can lay all fish eggs at a time, and therefore it is avoided that artificial oxytocic hormones have negative effects on the parent fishes, production service life of the parent fishes is prolonged, and quality of germ cells is improved. The problem that 'little amount and many times' of fish egg laying of the parent fishes occurs when the scaphesthes macrolep is bred by using a common method is solved. The parent fishes, having the naturally-maturing gonads, of the scaphesthes macrolepis can be successfully cultivated in the method, the egg laying rate of the parent fishes reaches 58.6%, and the hatching rate of the germ cells reaches 67.3%.

The embodiment of the invention discloses a sticky fish egg hatching device, and relates to the field of aquaculture equipment. The hatching device comprises a hatching box, wherein the top of the hatching box is provided with a sealing cover; a water outlet is formed in the bottom of the hatching box; the sticky egg hatching device also comprises a spraying device, a support frame device and a plurality of fish egg loading devices; the spraying device, the support frame device and the plurality of fish egg loading devices are respectively arranged in the sticky fish egg hatching box; the support frame device comprises a center shaft and a plurality of hanging support frames; the center shaft is in vertical arrangement; the plurality of hanging support frames are radially arranged at the periphery of the center shaft at intervals; the hanging support frames are used for hanging the fish egg loading devices; the spraying device is used for spraying water mist on the fish egg loading devices. The sticky fish egg hatching device provided by the embodiment has the advantages that the fish egg loading devices are hung through the support frame device; each fish egg loading device is hung in a separated way; the problem of oxygen deficiency due to fish egg overlying can be effectively solved; the fish egg hatching rate is improved. Through the arrangement of a temperature control system, the fish egg hatching temperature and humidity can be effectively controlled; the fish egg hatching rate is further improved.

The invention discloses a device and a method for automatically collecting fish eggs, comprising a box body, an iron gauze separator, a filter screen separator and waterweeds, wherein the iron gauze separator and the filter screen separator are arranged in the box body from top to bottom, the mesh diameter of a filter screen of the filter screen separator is smaller than the mesh diameter of an iron gauze of the iron gauze separator, a gap is formed between the filter screen separator and the bottom of the box body, a discharge port is arranged at the bottom of the box body, the iron gauze separator is horizontally inserted into a first group of slot, the filter screen separator is horizontally inserted into a second group of slot, the first group of slot and the second group of slot are horizontally arranged on the side surface of the box body, which is vertical to a handle of the iron gauze separator, and the waterweeds are horizontally arranged on the top of the iron gauze separator. The device and the method for automatically collecting fish eggs have the beneficial effects that the device is simple in structure, low in price and has less influence on adult fish, the egg laying rate is high, the injury to fish eggs is reduced, the fertility rate is increased, the labor intensity is relieved, and the working efficiency is improved.

The invention relates to a symphodon discus breeding and fish larvae culturing method. The method includes the stages of fish larvae culturing, juvenile fish culturing and artificial bait culturing. By means of control of the variety of fed biological bait, feeding times, feeding time and water body culture conditions of a parent fish egg laying vat and a juvenile fish culture vat, requirements of fish larvae growth for water quality and nutrition can be effectively met, the survival rate of the fish larvae is improved, quick growth of the fish larvae is promoted and economic efficiency is increased.

The invention belongs to the field of aquaculture, and particularly relates to an application of neutral sterilization electrochemical water in fish egg hatching. The neutral sterilization electrochemical water which is efficient in sterilization, environmentally friendly, safe and free of bacteria, aquatic plants and insects is adopted to hatch fish eggs and hatch eggs of famous fish such as loach, sturgeon, rainbow trout and fancy carp, the fish egg hatchability and the survival rate can be effectively improved, chemicals are replaced, drug residues and aquatic product aquaculture water area environmental pollution are avoided, and the application and method are easy and convenient to operate and fast in efficacy, and have wide application prospects.

The invention discloses a seed production and culture method of a carp with short growth period and comprises the steps of the selection of breeding parent, which determines Jinxin female carp and Ukrainian scaled male carp as seed production parents through the RAPD technology, grouping of the male and female parents with a proportion of 1 to 1.2-1.5, reproduction of fish seeds by the methods of artificial flushing, natural spawning or artificial insemination, incubation in the step of which artificial fish egg collectors with naturally produced eggs and artificial fish egg collectors with fertilized ovum through artificial dousing are delivered to a pond for natural incubation or the fertilized ovum through artificial insemination is doused in the waxed pond bottom and then placed into a ring channel for incubation after being collected and washed, and initial culture and commercial fish culture: each Mu of pond can culture 2,000-2,200 pieces of summer fingerlings or 1200-1400 pieces of spring fingerlings and is matched with silver carp fingerlings or spring fingerlings which account for 20 percent of the main culture objects. The fish can be cultured in the current year. The carp with short growth period cultured by the method of the invention has the advantages of unique shape, rapid growth, strong disease resistance and high yield.

An artificial inducement method for spawning and breeding erythroculter ilishaeformis relates to an artificial inducement method for spawning and breeding fish. The method solves the problems of a low hatching rate and a low post-natal survival rate in the existing artificial reproduction method of the erythroculter ilishaeformis. The breeding method comprises the following steps: 1. culturing parent fish in a culture pond; 2. injecting an aphrodisiac agent into the parent fish twice so that the parent fish autogenously spawns; 3. transferring the fish eggs into a hatching barrel for hatching; and 4. transferring fish fry into an outdoor pond after opening an inflatable intestinal canal of a fish maw so as to culture young fishes. The artificial inducement method for spawning and breeding of the erythroculter ilishaeformis can help shorten a breeding period, greatly improve the survival rate and the hatching rate of the parent fish, and obviously improve the economic benefit.

The invention relates to an automatic collection device for semi-floating fish eggs. The automatic collection device is characterized in that the automatic collection device comprises a right-angle bent pipe, a suspension rope, a net bag and an inflating pipe, the right-angle bent pipe is suspended in an egg laying pond through the suspension rope and the suspension rope is tied at the bent position of the right-angle bent pipe; the right-angle bent pipe comprises a vertical pipe section and a transverse pipe section, a weight is arranged at the lower end of the vertical pipe section to keep the vertical pipe section to be in a vertical state when the right-angle bent pipe is in a suspended state; the mouth of the net bag is sheathed at the rear end of the transverse pipe section and is tightly tied through a tying rope; and the inflating pipe is inserted into the vertical pipe section by certain length from the bent position of the right-angle bent pipe. The automatic collection device for the semi-floating fish eggs has the advantages that the device is simple, convenient and feasible to operate, the manufacturing cost is low, the labor intensity can be reduced and the working efficiency is improved; the damage to the fish eggs is reduced, the collection rate of the fish eggs is high and the hatching rate of fish fries is high; and the structure is simple, the space of the egg laying pond is not occupied, the fish egg collection and the inflation can be conducted at the same time, additional investment is not required and the automatic collection device is particularly suitable for the artificial breeding of seawater and freshwater fishes such as four major domesticated fish and the like which lay semi-floating eggs.

The invention discloses a method for preparing fish egg sugar protein peptide with an anti-osteoporosis effect. The method comprises the following steps: performing freeze drying on the fish eggs, and performing ungrease treatment by using ethanol; performing freeze drying on the fish eggs subjected to ungrease treatment, adding protease for hydrolyzing, regulating pH value of the hydrolysate into neutral after the hydrolysis is ended, performing ultrafiltration and desalting the supernatant fluid of the centrifuged hydrolysate, concentrating and drying to obtain the fish egg sugar protein. The prepared fish egg sugar protein peptide can be used for preparing health-care food, functional food or medicines for preventing and treating osteoporosis. The used raw materials are rich and readily available, the product can perfectly keep the structure of sialic acid in the fish egg sugar protein, and the method is simple in process steps, low in equipment investment, free in environmental pollution and suitable for industrial production. The product has high anti-osteoporosis activity and can be develop the food or medicines with the effects of safely and effectively preventing and treating osteoporosis; and meanwhile, the defects of the development and utilization of fish egg sugar protein are overcome, and a novel way for high-valued processing of fish eggs is developed.

The invention relates to the field of aquaculture and aims at providing a fry incubation and rearing pond with fish spawning nests and improving the survival rate of fries. According to the technical scheme, the fry incubation and rearing pond with the fish spawning nests comprises a pond body, wherein the pond body is provided with a water outlet portion and a matched aeration structure, and air outlet holes of the aeration structure are evenly formed along the periphery of the water outlet portion. The aeration structure produces a large amount of air bubbles distributed around the water outlet portion. Therefore, the phenomenon that fries poor in swimming ability go to the surrounding of the water outlet portion, are sucked, cannot break away and accordingly are dead can be well prevented, and the survival rate of the fries is effectively improved. In addition, at an incubation stage, the aeration structure can play an aeration oxygenation role, necessary oxygen is provided for fry incubation, and higher incubation rate is ensured. Furthermore, the fry incubation and rearing pond is additionally provided with the fish spawning nests facilitating fish spawning, a water inlet pipeline and a water outlet system, wherein the water inlet pipeline and the water outlet system are convenient to use. The fry incubation and rearing pond is suitable for fish spawn incubation and rearing and is used in the field of aquaculture.

The invention relates to aquaculture equipment, in particular to a larva fish hatch device comprising a filter tank, a hatching tank and a drainage tank. The filter tank and the hatching tank are communicated with water sources, the drain tank lower than the hatching tank and is enclosed all around with an opening at the top is arranged in the hatching tank, and a drain port communicated with a drain pipe is formed in the drain tank. A hatching ring path is formed between the drain tank and the hatching tank, and the bottom of the hatching ring path is connected with the filter tank through a water inlet pipe. An egg resistance screen is fixed at the upper end face of the drain tank, and an upper angle of elevation is formed between the egg resistance screen and the upper end face of the drain tank. According to the arrangement, when water level in the hatching ring path reaches the upper end of the drain tank, the egg resistance screen at the upper end of the drain tank can prevent fish eggs discharging from the drain tank, so that flowing water in the hatching ring path can protect the fish eggs and improve hatching rate of larva fish. In addition, the drain tank is arranged under the water surface of the filter tank, so that water can be automatically ushered into the hatching ring path to keep the water flowing by the aid of water level difference, and accordingly, cost is saved.

The invention provides an artificial breeding method for wild schizothorax waltoni. The method comprises the following steps of 1, collecting parent fish, wherein parent schizothorax waltoni is collected at the Yarlung Zangbo River from the first ten-day period of April to the last ten-day period of May every year; 2, performing artificial spawning induction, wherein before spawning induction, anesthetic is adopted for anesthetizing the parent fish, and oxytocic is injected to female fish and male fish; 3, performing artificial insemination, wherein after the parent fish is anesthetized by theoxytocic, seminal fluid and eggs are collected, and dry-method insemination is adopted; 4, performing germ cell incubation, wherein before the myomere appearing period, germ cells are placed into anincubation frame for running water incubation; after the germ cells are developed to the myomere appearing period, dead cells are removed, and the germ cells are moved into a roe incubator for incubation; 5, opening fry. Large-scale artificial breeding of schizothorax waltoni is achieved.