121 results about "Intestinal digestion" patented technology

The invention provides a hypoglycemic peptide which is prepared by taking bovine whey protein as a raw material and is stable in gastrointestinal digestion and absorption. In an in-vitro cell experiment, the peptide fragment can improve the uptake of insulin-resistant HepG2 hepatocytes and synthesize glycogen by using glucose, can promote intestinal endocrine cells NCI-H716 to secrete GLP-1, has a certain synergistic effect, and can further play the effects of promoting GLP-1 secretion and activating a GLP-1 receptor through combined use. An insulin resistance mouse model is constructed by utilizing high-fat diet, and the peptide fragment is adopted for intervention, so that the hypoglycemic peptide is further proved to have obvious effects on losing weight, reducing lipid accumulation and assisting in reducing blood fat, and meanwhile, the hypoglycemic peptide can be used for improving insulin resistance related symptoms. The milk peptide provided by the invention has gastrointestinal digestion stability and hypoglycemic activity, can be used as a functional milk base material for developing food, medicines or health care products with the effect of regulating blood sugar, and is wide in application prospect.

The invention discloses a nutrition improvement nursing method for a gastrointestinal digestion patient, and relates to the field of medical monitoring, and the method comprises the following steps: analyzing to obtain the current absorption amount of to-be-supplemented nutrient elements of the gastrointestinal digestion patient; analyzing to obtain a health influence coefficient of the to-be-supplemented nutrient elements on the gastrointestinal digestion patient; acquiring an absorption promotion coefficient between the to-be-supplemented nutrient elements and other to-be-supplemented nutrient elements; forming a to-be-updated recipe of the current recipe; calculating a health contribution value of the to-be-updated recipe to the gastrointestinal digestion patient; and calculating the coincidence proportion of the to-be-updated recipe relative to the current recipe, and performing recipe change on the current recipe. According to the method, the health influence coefficient is obtained through analysis, the absorption promotion coefficient is obtained, the to-be-updated recipe is formed, the corresponding health influence is obtained according to the deficiency of different to-be-supplemented nutrient elements, and the mutual influence condition existing between the different to-be-supplemented nutrient elements is also considered, so that the prediction result of nutrient absorption is closer to the reality.

The invention discloses a method for improving gastrointestinal tolerance of probiotics and a probiotic liquid core gel ball, the probiotic liquid core gel ball is prepared by adopting a calcium alginate cross-linked reverse spheroidizing method, and various indexes influencing the characteristics of the probiotic liquid core ball after re-culture are optimized, the process is simple in preparation, raw materials are natural and safe, the cost is low, and the method is suitable for industrial production. The tolerance and the survival rate of the probiotics in gastrointestinal digestion can be well improved, and the probiotic liquid core gel ball prepared by the method disclosed by the invention has good mechanical property, swelling rate and storage survival rate; by optimizing various parameters, such as re-culture time, core material liquid viscosity, polysaccharide molecular weight and a storage solution, which influence the characteristics of the probiotic liquid core ball after re-culture, the re-cultured liquid core ball has better mechanical properties, swelling rate and survival rate, and by combining with a gastric acid-cholate resistance reaction result, the survival rate of the liquid core ball is improved. The invention provides the preparation process of the re-culture solution core ball capable of effectively improving the gastrointestinal tolerance of the probiotics whether the core ball is broken or not.

The invention belongs to the field of food processing, and discloses a preparation method and application of a targeted colon delivery polyunsaturated fatty acid grease microcapsule. 11S globulin in soybeans and Arabic gum are used as wall materials of the microcapsule, complex coacervation of the 11S globulin in the soybeans and the Arabic gum is induced under a certain pH condition, so that oil drops are wetted by a coacervate, a core-shell structure is formed, a coacervate phase is further stabilized through glucan to inhibit coalescence, and on the basis, the microcapsule is prepared. And tannic acid, polysaccharide and protein can form hydrogen bonds and generate hydrophobic interaction to further crosslink the condensed phase to obtain the microcapsule capable of resisting digestion of the stomach and the small intestine, so that colon delivery of polyunsaturated fatty acid is realized.

The invention belongs to the technical field of aquaculture, and particularly relates to a pearl grouper fry rearing method. According to the method, firstly, after the roes are hatched, the fries are soaked with a bactericide, harmful bacteria on the surfaces of the roes and in a hatching pond can be effectively killed, and a bacteria solution of lactobacillus brevis serves as the bactericide, so that the use of antibiotics is reduced while the growth of pathogenic bacteria is inhibited; the culture pond is divided into 1m * 1m spaces by the partition plates, so that the phenomenon that the fries kill each other or snatch for food due to insufficient spaces is avoided, and uniform growth of the fries is facilitated; culture is carried out in stages according to nutritional requirements of different growth stages, and the Weissella microbial inoculum and the lactococcus lactis microbial inoculum are added to the feed in each stage, so that the intestinal microecological balance of the fry is adjusted, the growth and reproduction of harmful bacteria are inhibited, the intestinal digestion and absorption functions of the fry are enhanced, and the utilization rate of the feed is increased.

The invention belongs to the field of fish breeding, and discloses a construction method and primers of a fast-growing red crucian carp homozygous strain, and the construction method comprises the following steps: preparing gRNA of a red crucian carp acvr2b gene, microinjecting a mixture of the gRNA and Cas9 protein into a one-cell stage fertilized egg of the red crucian carp, and screening to obtain an F0-generation gene knockout mutant; carrying out selfing on the F0-generation gene knockout mutant to obtain an F1-generation mutant, and screening to obtain a homozygous mutant; and selfing and propagating the homozygous mutant to obtain the product. The acvr2b homozygous knockout strain is successfully established in the red crucian carp through a CRISPR / Cas9 gene editing technology for the first time, the body weight of the obtained acvr2b mutant red crucian carp at the age of 12 months is increased by 30.68% compared with that of a wild type, the muscle protein content is increased by 13.81%, the intestinal digestion and absorption capacity is obviously improved, high-quality germplasm resources are provided for fish genetic breeding, and the acvr2b mutant red crucian carp has a good application prospect.

The invention belongs to the field of food nutrition and functional factors, and particularly relates to a method for preparing ovotransferrin hydrolysate with high immunocompetence based on an adult gastrointestinal simulated digestion system. According to the method, ovotransferrin is treated through the stomach and intestine of a simulated adult, OVT hydrolysate is prepared, and the OVT hydrolysate contains HTEGSTT, TTSY and / or RTAGWVIPMG. The invention finds that the immunomodulatory activity of OVT is enhanced after gastrointestinal digestion and hydrolysis, the OVT is more easily hydrolyzed in simulated adult digestive juice than in infant digestive juice, and a contrast experiment shows that the nutrition intervention of OVT adult and infant simulated digestion products can promote the generation of short-chain fatty acids such as acetic acid, propionic acid and butyric acid; the swelling injury caused by inflammation to small intestine mucous membrane tissues is effectively relieved, so that the intestinal barrier immune function is obviously improved, and the OIA shows higher intestinal immune activity than the OIB.

The invention belongs to the technical field of biological small molecule active peptides, and more specifically relates to a uric acid reducing active peptide, a preparation method and an application thereof. The uric acid reducing activity LQKW has remarkable XOD inhibitory activity, IC50 is 2.70 mg / mL, and the IC50 is superior to that of many known natural source XOD inhibitory peptides; good gastrointestinal digestion stability is shown, and the activity retention rate after in-vitro simulated gastrointestinal digestion is (61.84 + / -0.82)%. According to the invention, a specific binding mechanism of uric acid reduction activity and XOD active sites (Ile648, Phe649 and the like) is clarified through molecular docking, and a basis is provided for rational design based on a structure. According to the invention, the method for efficiently preparing the active peptide fragment from the white spirit vinasse is established, and the high-valued utilization of the wine brewing byproducts is realized. The uric acid reducing activity LQKW provided by the invention can be prepared through solid-phase synthesis, the process is mature, the quality is controllable, and a foundation is laid for industrialization. In addition, as the XOD inhibitor of a natural source, the uric acid reducing activity LQKW has good safety.

The invention discloses a glycinin compound for preventing hyperlipemia as well as a preparation method and application of the glycinin compound, and belongs to the field of biological medicines. The preparation method comprises the following steps: stirring and mixing resveratrol and a soybean 7S globulin solution at 40-60 DEG C for 1.5-2.5 h, so that resveratrol and soybean 7S globulin are fully combined to obtain a glycinin compound; the mass ratio of the soybean 7S globulin to the resveratrol is (250: 50)-(250: 45). Experimental results show that the glycinin compound disclosed by the invention can inhibit the weight increase of a hyperlipemia rat, remarkably reduce the fat index and the liver index, effectively improve the abnormal change of the blood fat factor level and show an obvious lipid-lowering effect. In-vitro simulated digestion experiments show that the glycinin compound shows peptide fragments with different strength from soybean 7S protein after gastrointestinal digestion, and meanwhile, the glycinin compound improves the in-vitro cholate binding capacity.

The invention discloses a method for adjusting functional properties and oxidation resistance of potato protein, and relates to the technical field of food processing. The method comprises the following steps: extracting potato protein by adopting an alkali-solution and acid-isolation method, and modifying the potato protein by utilizing an ultrasonic technology; the ultrasonic frequency is set to be 20 kHz, the amplitude is set to be 40%, the ultrasonic time is 0-10 min, and the change rule of the potato protein structure and the functional property under different ultrasonic time is analyzed. After ultrasonic treatment, the particle size and polydispersity index of the potato protein are obviously reduced, the Zeta-potential is increased, but main strips are not changed; after 6 minutes of ultrasonic treatment, the particle size is 266.90 + / -2.26 nm, the PDI is 0.27 + / -0.03, and the Zeta-potential is-26.25 + / -0.93 mV. After 6 minutes of ultrasonic treatment, the solubility of potato protein is the highest, the foamability is improved by 1.18 times, the emulsifying activity is improved by 1.54 times, and the digestibility of simulated intestines is remarkably improved. Under the condition, the reducing power of the potato protein and the scavenging rates of DPPH, superoxide anions and hydroxyl radicals reach the maximum value.

The invention discloses a preparation method of a red rice bran protein peptide compound with antioxidant and hypoglycemic effects. According to the method, red rice bran protein is extracted through an alkali-solution and acid-isolation method; hydrolyzing the red rice bran protein with alkaline protease to obtain a red rice bran protein hydrolysate; the red rice bran protein hydrolysate prepared by the invention has the functional characteristics of excellent emulsibility, foamability and oil absorbency, and has excellent antioxidant and hypoglycemic functional activities after being subjected to in-vitro simulated gastrointestinal digestion. The red rice bran protein hydrolysate can be used in plant-based food and dairy product substitutes and functional food with a hypoglycemic effect.

The present invention relates to the technical field of whey protein hydrolysis, and particularly to a preparation method and application of buffalo colostrum-derived bioactive peptides. The present invention optimizes the enzymatic hydrolysis process of buffalo colostrum whey protein through single-factor experiments to obtain an enzymatic hydrolysis method of buffalo colostrum whey protein suitable for large-scale production. In addition, the enzymatic hydrolysis process of buffalo colostrum whey protein is optimized by the response surface method. Three factors, namely the pH value, enzymatic hydrolysis time, and enzyme addition amount during enzymatic hydrolysis with neutral protease, are selected as corresponding variables, and the ABTS radical scavenging rate is used as the response value. Multiple fittings are performed on each factor to obtain the optimal enzymatic hydrolysis process conditions for whey protein. The buffalo colostrum-derived bioactive peptides obtained by this enzymatic hydrolysis method have the technical advantages of high ABTS radical scavenging rate and good immune function. At the same time, the stability of the enzymatic hydrolysis product is further studied through gastrointestinal digestion experiments, providing a more comprehensive theoretical reference for the further research of whey protein.

The application discloses a South China Sea Decapterus maruadsi polypeptide with uric acid reducing and antioxidant activities and an application thereof, and relates to the technical field of biology. The polypeptide with high uric acid reducing activity and antioxidant activity is screened through activity prediction and molecular docking, and the amino acid sequence is FYDWWK. The polypeptide can be stably combined with the active center of xanthine oxidase, forms multiple hydrogen bonds and hydrophobic interaction, significantly inhibits the generation of uric acid, and has an in-vitro xanthine oxidase inhibition IC 50 of 3.068±0.2547 mmol / L. Meanwhile, the polypeptide has excellent DPPH free radical scavenging capacity, and an IC 50 of 5.033±0.5476 mmol / L. The polypeptide has good stability, still has high efficient inhibitory activity after simulated gastrointestinal digestion, and has oral development potential. The polypeptide is a natural marine small molecule active peptide, is safe and easy to absorb, and can be applied to the preparation of products related to uric acid reducing and / or antioxidant.

The invention discloses a preparation method and application of 7-hydroxyisoflavone acetylferulate, relating to the technical field of feed additives. The preparation method comprises the following steps: adding acetylferulic acid, 7-hydroxyisoflavone, 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride, and N,N-dimethyl-4-pyridinamine to a reactor; using dichloromethane as a solvent; stirring at room temperature for 5 hours; adding water after the reaction is complete; and then extracting with dichloromethane; and concentrating and crystallizing the product to obtain a target product. The product prepared by this method has good fat solubility and stability, and can be added as an additive to livestock, poultry, and aquatic animal feed to significantly improve the growth performance, antioxidant capacity, immunity, and intestinal digestion capacity of the animal.

1. The name of the design product: ultrasonic digestive endoscope. 2. The use of the design product: for gastrointestinal digestive examination diagnosis and treatment. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The present invention provides a package of a micronutrient supplement for promoting the digestive function of the intestinal tract of a body, the micronutrient supplement consisting of two types of unit dosage forms in one package for sale to a purchaser wherein the first type of unit dosage form contains VB6 and a compound thereof, and the second type of unit dosage form contains VB6 and a compound thereof. The first type of unit dosage form contains Zn and compounds thereof, the second type of unit dosage form contains Zn and compounds thereof or VD and compounds thereof, and the first type of unit dosage form does not contain Zn and VD, and the second type of unit dosage form does not contain VB6, the micronutrient supplement being provided in a manner of administration twice a day, the first type of unit dosage form is taken in an awakening period, and the second type of unit dosage form is taken in a sleep period. The packaging product can be matched with the biological clock rhythm of an organism, the absorption and utilization rate of micronutrients is increased, and then the intestinal digestion function is improved.

The invention belongs to the technical field of whey protein processing, and particularly relates to a preparation method of a hydrolyzed whey protein digestion product with high immunocompetence and low sensitization. The preparation method comprises the following steps: by taking hydrolyzed whey protein as a raw material, carrying out simulated gastrointestinal digestion treatment under specific conditions to obtain a whey protein digestion product; the product can effectively exert immunoregulation and anti-inflammatory effects, solves the problem of sensitization of a traditional whey protein product, and has a good application prospect.

The invention discloses a preparation method of a whey protein / pullulan / sugar astaxanthin nano-dispersion, WPC / PUL compounds are adopted to construct different carriers to embed AST, the carriers are respectively a nano-dispersion loaded with AST, emulsion gel and microcapsule powder, the three carriers have good embedding efficiency and protection effect on AST, the simulated gastrointestinal digestion stability of AST is remarkably improved, and the stability of AST is improved. And the method has great significance in expanding the preparation of an AST embedding delivery system and the application of AST in industries such as food and the like.

The invention discloses a black pepper extract-containing composition capable of promoting intestinal digestion and absorption and a cat food, and relates to the technical field of animal foods.The composition is prepared by compounding fish oil, krill oil, sunflower seed oil and linseed oil according to a specific proportion based on compound functional grease and natural plant extracts in a scientific proportion; according to the invention, the ratio of omega6 fatty acid to omega3 fatty acid is optimized to an ideal range of (2.5-3.2): 1, black pepper extract with functions of promoting digestion and regulating flora is synergistically added, and through combination of scientifically proportioned functional components and an advanced process, an'intestinal tract-microorganism-metabolism 'experiment verifies that digestion and absorption of cat nutrition are realized, and the effect of promoting digestion and regulating flora is achieved. Meanwhile, the multi-dimensional and systematic improvement of oxidation resistance, inflammation resistance, immunity improvement and metabolism functions is achieved.

The invention relates to the technical field of fermented fruit puree, in particular to infant fermented fruit puree and a preparation method thereof. The fruit paste is prepared by the method. The method comprises the following steps: preparing fruit paste; adding a fermentation inoculant and fructosyl transferase into the fruit paste, and synchronously performing fermentation and enzymolysis to obtain fermented fruit paste; adding transglucosidase into the fermented fruit paste, and carrying out secondary enzymolysis treatment; carrying out sterilization and enzyme deactivation treatment on the fermented fruit paste subjected to the secondary enzymolysis treatment; carrying out homogenization treatment on the fermented fruit paste subjected to sterilization and enzyme deactivation treatment; and sequentially canning and sterilizing the homogenized fermented fruit paste to obtain the infant fermented fruit paste product. According to the invention, the puree product with rich taste and flavor can be prepared, the nutritional value of the puree product is improved, the sugar reducing effect is realized to a certain extent, and intestinal digestion and probiotic proliferation are promoted.

The invention relates to a method for preparing colla corii asini oligopeptide by adopting a dynamic bionic digestive system, which comprises the following steps: preparing digestive juice simulation liquid which comprises oral cavity digestive juice, stomach digestive juice and intestinal digestive juice; the donkey-hide gelatin peptide sample simulates oral digestion; starting a dynamic human body bionic digestion system, adding a sample digested by an oral cavity into the system, adding gastrointestinal digestion liquid into the system, and simulating a dynamic gastrointestinal digestion process; collecting gastrointestinal digestion products, carrying out water bath enzyme deactivation, cooling, centrifuging, separating supernate and precipitate, and detecting molecular weight distribution of the supernate; and freeze-drying the supernate into powder to obtain the donkey-hide gelatin oligopeptide. The colla corii asini peptide is treated by adopting a dynamic bionic digestive system, the digestion process of a human body is simulated, and the colla corii asini oligopeptide is obtained, so that blood-enriching active substances in the colla corii asini peptide are better released, human body absorption is facilitated, and the bioavailability and the blood-enriching effect of the colla corii asini peptide are improved.

The invention belongs to the technical field of food processing, and relates to a preparation method and application of astaxanthin emulsion gel. The invention provides a preparation method of astaxanthin emulsion gel, which comprises the following steps: taking an ovalbumin and beet pectin compound solution obtained through electrostatic interaction under a specific pH value as a water phase, taking medium-chain fatty acid containing astaxanthin as an oil phase, and mixing the oil phase with the ovalbumin and beet pectin compound solution to obtain the astaxanthin emulsion gel. The preparation method comprises the following steps: carrying out homogenization treatment on a blend of a water phase and an oil phase by using a high-speed dispersion machine to obtain a primary emulsion, carrying out ultrasonic treatment on the primary emulsion to obtain an emulsion, adding calcium ions into the emulsion, uniformly mixing, and carrying out crosslinking and phase change on the emulsion to obtain the astaxanthin emulsion gel. According to the astaxanthin emulsion gel disclosed by the invention, the storage stability of the astaxanthin can be remarkably improved, so that more astaxanthin is migrated to a water phase in an intestinal digestion process, the bioavailability of the astaxanthin is improved, and the antioxidant activity in vivo is remarkably improved compared with that of free astaxanthin.

The invention discloses a gastrointestinal ultrasound contrast agent without scattering echoes as well as a preparation method and application thereof, and relates to the technical field of biomedical ultrasound. The gastrointestinal ultrasound contrast agent comprises a thickening agent, a sound velocity regulator and water, the thickening agent comprises an edible gum thickening agent, and the edible gum thickening agent is selected from one or more of xanthan gum, guar gum, konjac gum, gellan gum, sodium carboxymethyl cellulose, Arabic gum, modified starch and natural starch; the sound velocity regulator is selected from one or more of skim milk powder, polyethylene glycol and mannitol. The gastrointestinal ultrasound contrast agent disclosed by the invention has the characteristics of no scattering echo and relatively low sound attenuation, and is suitable for clinical requirements of ultrasound examination and screening of gastrointestinal digestive tracts. For focuses, with high echoes, of all layers of the stomach wall or gaps of the stomach wall in the stomach wall, the gastrointestinal ultrasound contrast agent without scattering echoes has a very good contrast imaging effect.

This invention discloses a method for evaluating the bioavailability of soybean oil based on an in vitro digestion / cell co-culture model. Soybean oil is emulsified using WPI emulsifier and then digested sequentially through simulated gastric and intestinal fluids to obtain the gastric digestion products of soybean oil. Fatty acids are extracted from the intestinal digestion products of soybean oil, dissolved in dimethyl sulfoxide, and linked to fatty acid-free bovine serum albumin to obtain in vitro digestion products of soybean oil that can be absorbed by the intestines. These are then added to a cell co-culture model for cell incubation and transport. HepG2 cells from the cell co-culture model are collected, and Oil Red O staining and total triglyceride content are measured to obtain lipid deposition, thereby evaluating the bioavailability of soybean oil. The in vitro digestion / cell co-culture model of this invention is specifically designed for soybean oil digestion. This method objectively and scientifically evaluates the bioavailability of soybean oil at different metabolic stages, providing a standard basis for the scientific and healthy consumption of soybean oil and possessing significant practical value.

The invention provides an antibacterial peptide for inhibiting food-borne pathogens as well as an acquisition method and application of the antibacterial peptide, and relates to the technical field of bioactive peptides and biomedical engineering. The amino acid sequence of the antibacterial peptide is as follows: Gly-Leu-Thr-Leu-Lys-His-Leu-Lys-Lys-Leu-Phe, and the antibacterial peptide can be used for effectively inhibiting the growth of food-borne pathogens such as staphylococcus aureus, salmonella, escherichia coli, pseudomonas aeruginosa, listeria monocytogenes and the like. The defects in the prior art are overcome, the antibacterial peptide with antibacterial activity is found and identified through a screening method combining a gastrointestinal digestive enzyme-peptide omics technology and an experiment, and a new natural antibacterial agent is provided for food, medicine and other related fields.

The application belongs to the field of fish breeding, and discloses a construction method and primers of a homozygous line of fast-growing red crucian carp. acvr2b The construction method comprises the following steps: preparing gRNA of a red crucian carp gene, microinjecting a mixture of the gRNA and a Cas9 protein into a one-cell fertilized egg of the red crucian carp, screening to obtain F0 generation gene knockout mutants, self-crossing the F0 generation gene knockout mutants to obtain F1 generation mutants, screening to obtain homozygous mutants, and self-crossing the homozygous mutants to propagate. acvr2b The application successfully establishes a homozygous knockout line in the red crucian carp for the first time through a CRISPR / Cas9 gene editing technology. acvr2b The mutant red crucian carp obtained in the application has a body weight increased by 30.68% and a muscle protein content increased by 13.81% compared with a wild type at 12 months of age, and has a significantly improved intestinal digestion and absorption capacity, thereby providing high-quality germplasm resources for fish genetic breeding and having a good application prospect.

The present invention discloses a Chinese clam antihypertensive peptide and its application in the preparation of antihypertensive drugs and health foods. The Chinese clam antihypertensive extract is obtained by enzymatic hydrolysis, and the extract is further separated and purified to obtain a polypeptide of a specific sequence, and the amino acid sequence of the antihypertensive peptide is Lys-Asp-Glu-Tyr-Lys-His-Leu-Asn-Phe-Gly-Glu-Val (KDEYKHLNFGEV). The Chinese clam antihypertensive peptide disclosed in the present invention has significant angiotensin converting enzyme (ACE) inhibitory activity. In vitro, it is manifested as temperature, pH, simulated gastrointestinal digestion and angiotensin converting enzyme (ACE) degradation stability. It shows good antihypertensive effect both in vitro and in non-spontaneous hypertensive rats (SHRs), and can be used in health products and medicines related to hypertension treatment.

The invention discloses application of compound probiotics to improvement of intestinal digestive ability of Debao pony and a preparation. The compound probiotics are composed of bacillus, saccharomycetes and lactic acid bacteria according to the ratio of 8: 7: 0.5, and 50-70 mL / piece of compound probiotics is added into drinking water every day. By adjusting the intestinal flora structure, the relative abundance of ackermania and wart micromycota is remarkably reduced, the proliferation of Alamiaceae RC9, Heliraceae XPB1014 / AC2044, anaerobic vibrio and glycozymophyte is promoted, and the digestive ability of the foals to crude fibers and non-fibrous carbohydrates is improved. By means of the application, the apparent digestibility of CP, the apparent digestibility of NDF and the apparent digestibility of ADF are improved by 7.57%, 10.66% and 10.82% respectively, the average daily gain is improved by 8.08%, and the feed-to-weight ratio is reduced by 4.59%. The invention provides a high-efficiency and low-cost solution for large-scale healthy breeding of Debao pony.

The invention relates to a preparation method of glucose oxidase double-layer gel microspheres with slow, continuous and remarkable increase of enzyme release amount in an intestinal digestion stage, and provides a product which is high in encapsulation efficiency, good in slow release effect in the whole gastrointestinal stage, resistant to high temperature and excellent in storage stability and swelling degree. According to the invention, sodium alginate, sodium carboxymethyl cellulose, chitosan and calcium ions are effectively utilized to carry out secondary coating on glucose oxidase, so that the mechanical strength, thickness and pore size of the membrane are changed, and the slow release performance is further improved. Compared with a single-layer gel microsphere, the double-layer gel microsphere can still continuously, remarkably and linearly release (Plt; 0.05) at the release amount of 0.15-0.2% per minute within 210-240 minutes at the intestinal digestion stage of people or animals; after the double-layer gel microspheres are placed at 80 DEG C for 10 minutes, the double-layer gel microspheres still have 39.76% of enzyme activity; 80.52% of activity can still be maintained after storage for 30 days; the glucose oxidase gel microspheres have wide application prospects in the field of food and feed processing.

This invention discloses a slow-digesting quinoa flour-concentrated milk protein composite gel food and its preparation method, belonging to the field of food processing technology. The invention first protects the composition as a gel food prepared from raw materials containing concentrated milk protein and quinoa flour; the preparation includes premixing quinoa flour and a portion of concentrated milk protein, hydrating them to obtain a premix with a starch-protein network, adding the remaining raw materials, stirring to form a slurry, and then shaping it. The gel food of this invention constructs a dense and continuous starch-protein network. This network, on the one hand, acts as a physical barrier to prevent effective contact between gastrointestinal digestive enzymes and internal proteins, maintaining the stability of the protein's secondary structure, thereby slowing down the rate of protein digestion and hydrolysis in the gastrointestinal tract to achieve slow digestion; on the other hand, it directly improves the product's hardness, chewiness, and other textural parameters, enhances water-holding capacity, reduces water separation rate, optimizes color and flavor, and achieves a synergistic improvement in multiple indicators of edible quality.