20 results about "Methylglyoxal" patented technology

The purpose of the present invention is to provide a beer-taste alcoholic beverage having a rich flavor, a favorable aftertaste, and a favorable amount of flavor. The present invention provides: a beer-taste alcoholic beverage in which the total concentration of free carboxymethyllysine (CML), free carboxyethyllysine (CEL), and free methylglyoxal-derived hydroimidazolone-1 (MG-H1) is 280-1,400 ppb; and a beer-taste alcoholic beverage in which the total concentration of free CML, free CEL, and free MG-H1 is 280-1,600 ppb, the total concentration of bound CML, bound CEL, and bound MG-H1 is 150-900 ppb, and the bound CML, the bound CEL, and the bound MG-H1 are included in a peptide fraction having a molecular weight of 1,060-3,080 Da (gel filtration method for HPLC analysis).

The purpose of the present invention is to provide a beer-flavored beverage that has rich taste and delivers refreshing stimulation. The present invention provides a beer-flavored beverage in which: the total concentration of free carboxymethyllysine (CML), free carboxyethyllysine (CEL), and free methylglyoxal-derived hydroimidazolone-1 (MG-H1) is 200 ppb to 1,750 ppb; the total concentration of bound CML, bound CEL, and bound MG-H1 is 33 ppb to 160 ppb; and the bound CML, bound CEL, and bound MG-H1 are contained in a peptide fraction having a molecular weight of 35–50 kDa.

The inventors have identified new peptide biomarkers related to methylglyoxal (MGO)-induced glycation of plasma albumin, as well as the most abundant plasma apolipoproteins. Plasma concentrations of MGH-APOB1003184-3194, MGH-ALB219-225, CEL-APOA229-39 were higher in patients with type 2 diabetes (T2D). After follow-up, cases of major adverse cardiovascular events (MACE), cases of severe coronary artery disease (CAD) events, cases of cardiovascular (CV) deaths and cases of all-cause deaths were reported in The SURDIAGENE study (n = 1425). Increased plasma concentrations of MGH-APOB1003184-3194,MGH-ALB219- 225, CEL-APOA229-39 were significantly associated with an increased risk of developing MACE, CAD, CV death, and / or CV death. Thus the present invention refers to a method of predicting the risk of having or developing a cardiovascular event in a patient.

The present invention relates to the field of thiabendazole preparation, specifically an improved thiabendazole preparation method. Comprise the following steps: S1 methylglyoxal is added to a container, the temperature is raised to 30-35 DEG C to melt, a catalyst is added, stirred evenly, air is blown in, and then o-phenylenediamine and acetic acid are added in batches, and the temperature is raised to 50-60 DEG C after adding, and the reaction is carried out for 2-5h to obtain a reaction solution containing 2-acetylbenzimidazole; S2 The reaction solution obtained in step S1 is added with liquid bromine, reacted, and after the reaction is completed, cooled, filtered, washed, and dried to obtain 2-dibromoacetylbenzimidazole hydrobromide; S3 The 2-dibromoacetylbenzimidazole hydrobromide obtained in step S2 is added to ethyl acetate, and then formamide and phosphorus pentasulfide are added for ring-closure reaction to obtain a closed-loop reaction liquid; S4 The thiabendazole in the closed-loop reaction liquid obtained in step 3 is purified to obtain a thiabendazole product. The process is simple, the prepared product has high purity, and the purity is 99.3%, and it is expected to be industrialized as soon as possible.

Disclosed is a beer-taste alcoholic beverage in which the total content of free carboxy methyl lysine (CML), free carboxy ethyl lysine (CEL), and free methylglyoxal-derived hydroimidazolione-1 (MG-H1) in the beverage is 600-1500 ppb, the total content of bound CML, bound CEL, and bound MG-H1 in the beverage is 90-349 ppb, the bitter taste value is 20 or more, and the content of the bound CML, bound CEL, and bound MG-H1 in the beverage is 90-349 ppb. In the beer-taste alcoholic beverage, the binding type CML, the binding type CEL, and the binding type MG-H1 are included in a peptide fraction having a molecular weight of 400-3000 Da. In the beer-flavored alcoholic beverage, unpleasant bitter taste is relieved.

Disclosed is a non-alcoholic beer-taste beverage in which the total content of free carboxy methyl lysine (CML), free carboxy ethyl lysine (CEL), and hydroimidazolione-1 (MG-H1) derived from free methylglyoxal in the beverage is 160 ppb or more. The mellow feeling of the non-alcoholic beer-taste beverage is enhanced.

The application discloses a kind of bifunctional carbon-based catalyst and its application, the bifunctional carbon-based catalyst of the particle size 1nm-7 μm boron-carbon-based microspheres, with furan boronic acid and methylglyoxal as raw material to prepare by hydrothermal co-carbonization reaction.It is with methylglyoxal and furan boronic acid mixture as composite functional carbon source, by one-step hydrothermal co-carbonization method synthesized with strong adsorption boron hydroxyl and in-situ carboxyl bifunctional carbon-based catalyst.Boron hydroxyl is adsorption site, by with cellulose form chemical bond force to improve the mass transfer efficiency of catalyst and cellulose;With in-situ carboxyl as catalytic site, break glycosidic bond, can realize cellulose to glucose efficient conversion in pure aqueous phase.In addition, the bifunctional carbon-based catalyst of the application has excellent hydrothermal stability and repeatability in catalyzing cellulose conversion, solvent system is water, more economical, green, safe.

The inventors have identified new peptide biomarkers related to methylglyoxal (MGO)-induced glycation of plasma albumin, as well as the most abundant plasma apolipoproteins. Plasma concentrations of MGH-APOB1003184-3194, MGH-ALB219-225, CEL-APOA229-39 were higher in patients with type 2 diabetes (T2D). After follow-up, cases of major adverse cardiovascular events (MACE), cases of severe coronary artery disease (CAD) events, cases of cardiovascular (CV) deaths and cases of all-cause deaths were reported in The SURDIAGENE study (n = 1425). Increased plasma concentrations of MGH-APOB1003184-3194,MGH-ALB219- 225, CEL-APOA229-39 were significantly associated with an increased risk of developing MACE, CAD, CV death, and / or CV death. Thus the present invention refers to a method of predicting the risk of having or developing a cardiovascular event in a patient.

The present application provides a method of producing 2-acetylpyrroline, comprising reacting 2-carboxypyrrline with methylglyoxal. The present application also provides a polypeptide comprising an amino acid sequence set forth in SEQ ID NO: 1 or a functional variant thereof, wherein the functional variant has delta(1)-pyrroline-2-carboxylate reductase activity. The present application also provides a method of producing 2-carboxypyrrline, comprising contacting the polypeptide with proline.

The invention relates to a methyl glyoxal preparation device and method. The device comprises a supply system and a reaction flow pipe system, wherein the supply system is connected with the reaction flow pipe system; the supply system comprises a chlorine supply module, a hydroxyacetone supply module and an oxygen supply module; the reaction flow pipe system comprises a reaction flow pipe, and the reaction flow pipe comprises a first connecting section, a second connecting section and a third connecting section; a photolysis module is arranged on the periphery of the first connecting section and comprises a light source assembly and a light reflection enhancing assembly. The light reflection enhancing assembly is arranged on the light propagation path; the chlorine supply module is connected with the first connecting section, the hydroxyacetone supply module is connected with the second connecting section, and the oxygen supply module is connected with the third connecting section. The device is simple and convenient to operate and mild in reaction condition, continuous flow synthesis can be realized, and the reaction efficiency and the product yield are remarkably improved.

The application relates to a method for continuously preparing methylglyoxal based on a glycerol gas-phase oxidation reaction, which comprises the following steps: subjecting pure glycerol or a glycerol solution to vaporization treatment to form raw material vapor; mixing the raw material vapor with O2-containing gas to form mixed gas; and contacting the mixed gas with a supported nanometer gold catalyst to generate selective oxidation reaction to form methylglyoxal, wherein the carrier of the supported nanometer gold catalyst is a metal oxide.

The invention belongs to the technical field of analysis and detection, and relates to a preparation method and a detection method of a pyruvic aldehyde electrochemical sensor based on an electro-activated gold electrode. The invention aims to solve the problems that the preparation method of the pyruvic aldehyde electrochemical sensor is complicated, the adopted material is expensive, the consumed time is long and the reproducibility is poor. The method comprises the following steps: polishing a bare gold electrode (AuE) on polishing cloth by using Al2O3 powder with the diameter of 0.05 mu m to form a mirror surface, washing the mirror surface by using distilled water, placing the prepared electrode in a K3 [Fe (CN) 6] solution in a range of 0-0.6 V at a scanning speed of 50 mV / s, testing by using a cyclic voltammetry, if a redox peak potential difference is within 80 mV, carrying out the next step, otherwise, repeating the above steps, and placing the qualified electrode in a KOH solution with the concentration of 0.1 M, carrying out electrical activation by adopting a cyclic voltammetry with the potential range of-2.0-2.0 V, circulating for 150 circles, placing the prepared electrode in a blank PBS solution, scanning for two circles by adopting the cyclic voltammetry, washing with distilled water, and detecting by adopting a certain detection method.

The present invention belongs to the technical field of genetic engineering and fermentation engineering, and in particular relates to a method for preparing S-lactoylglutathione, wherein glutamate, glycine, cysteine and methylglyoxal are used as raw materials, and are converted into S-lactoylglutathione under the catalysis of glutathione synthetase and glyoxalase. According to the present invention, the raw materials with relatively low cost are used for fermentation, the operation is simple, the conversion rate is high, the yield of the prepared S-lactoylglutathione is high, and the method is suitable for batch industrial production.

The invention discloses a supramolecular polymer fluorescence sensor. Fluorescence recognition is carried out on a tetraacethydrazide functionalized tetraphenylethylene derivative and a polymer of copper ions in a DMSO (Dimethylsulfoxide) solution. The sensor almost has no fluorescence in a solvent DMSO, and aqueous solutions of 2-chlorobenzaldehyde, 3-fluorobenzaldehyde, 3-chlorobenzaldehyde, 3-bromobenzaldehyde, 4-bromobenzaldehyde, crotonaldehyde, benzaldehyde, 4-fluorobenzaldehyde, p-tolualdehyde, p-nitrobenzaldehyde, heptanal, methyl glyoxal, 80% acetaldehyde and formaldehyde are respectively added into the solution; only formaldehyde can enable fluorescence of the sensor to be turned on. Besides, the sensor test paper displays blue-violet fluorescence under a 365 nm ultraviolet lamp, when the test paper is coated with formaldehyde aqueous solutions with different concentrations (10 <-1 >-10 <-8 > M), the test paper displays the change from light blue-green to dark blue under the 365 nm ultraviolet lamp according to the concentration of formaldehyde from high to low, and the sensor test paper can be used for recognizing formaldehyde solutions. In addition, the sensor can be used for adsorption of a formaldehyde solution, and the adsorption can be analyzed by a spectrophotometric method of acetylacetone. Therefore, the supramolecular polymer and the test paper thereof can be used for fluorescence recognition and formaldehyde adsorption. The fluorescence recognition and adsorption performance has important application value in the field of molecular recognition.

The present invention discloses a fluorescent probe for detecting methylglyoxal with a large Stokes shift, a preparation method thereof, and an application thereof, and belongs to the field of chemical analysis and detection technology. The fluorescent probe for detecting methylglyoxal has the structural formula: . In addition, the present invention also proposes a method for preparing the above-mentioned fluorescent probe for detecting methylglyoxal, comprising: dissolving compound C-3 in a first solvent, then adding concentrated hydrochloric acid and heating to 75-85°C for reflux reaction to obtain an intermediate BH-PDO; dissolving the intermediate BH-PDO in a second solvent, then adding stannous chloride dihydrate and concentrated hydrochloric acid for reflux reaction to obtain the fluorescent probe. In addition, the present invention also proposes a fluorescent probe prepared by the above-mentioned preparation method. In addition, the present invention also proposes the application of a fluorescent probe in detecting methylglyoxal. The fluorescent probe proposed by the present invention has a large Stokes shift and strong anti-interference ability, and can specifically detect methylglyoxal.

The invention discloses a fluorescent probe for recognizing methylglyoxal as well as a preparation method and application of the fluorescent probe, and belongs to the technical field of chemical analysis and detection. The structural formula of the fluorescent probe for identifying methylglyoxal is shown in the specification. In addition, the invention also provides a preparation method of the fluorescent probe. In addition, the invention also provides an application of the fluorescent probe or the fluorescent probe prepared by the preparation method in detection of methylglyoxal in an environment or a biological sample. The fluorescent probe provided by the invention can be used for detecting methylglyoxal in living cells and zebra fish, and can realize distribution imaging and detection of MGO in an arabidopsis thaliana living body in a lossless manner.

This invention relates to a composition for preventing and controlling internal rot in chestnuts and enhancing drought resistance, and its application, belonging to the field of pest and disease control technology. This invention provides a composition for preventing and controlling internal rot in chestnuts, comprising azoxystrobin, aminoethyl ester, calcium nitrate, gibberellin, methylglyoxal, a compound essential oil, and water. In this composition, aminoethyl ester and calcium nitrate work synergistically to further accelerate the penetration effect of azoxystrobin. The compound essential oil is formulated to target specific pathogens of internal rot, and together with azoxystrobin, it can eliminate pathogens of internal rot and also has a water-retaining effect. The addition of methylglyoxal can alleviate oxidative damage caused by drought stress. Spraying the composition of this application during the peak flowering period of chestnuts not only has a significant control effect on internal rot in chestnuts but also improves the drought resistance of chestnut trees.

Disclosed is a low-sugar beer-flavor alcoholic beverage in which the total content of free carboxy methyl lysine (CML), free carboxy ethyl lysine (CEL), and hydroimidazolione-1 (MG-H1) derived from free methylglyoxal in the beverage is 434 ppb or more. The mellow feeling of the low-sugar beer-flavor alcoholic beverage is enhanced.

The invention discloses a fluorescent probe for detecting methylglyoxal with large Stokes shift as well as a preparation method and application of the fluorescent probe, and belongs to the technical field of chemical analysis and detection. The structural formula of the fluorescent probe for detecting methylglyoxal is # imgabs0 #. In addition, the invention also provides a preparation method of the fluorescent probe for detecting methylglyoxal, which comprises the following steps: dissolving a compound C-3 in a first solvent, then adding concentrated hydrochloric acid, heating to 75-85 DEG C, and carrying out reflux reaction to obtain an intermediate BH-PDO; and dissolving the intermediate BH-PDO in a second solvent, and then adding stannous chloride dehydrate and concentrated hydrochloric acid for reflux reaction to obtain the fluorescent probe. In addition, the invention also provides the fluorescent probe which is prepared by the preparation method. In addition, the invention also provides an application of the fluorescent probe in detection of methylglyoxal. The fluorescent probe provided by the invention has the advantages of large Stokes shift and strong anti-interference capability, and can specifically detect methylglyoxal.

The present invention relates to a beer-taste alcoholic beverage in which the total content of free carboxy methyl lysine (CML), free carboxy ethyl lysine (CEL), and hydroimidazolione-1 (MG-H1) derived from free methylglyoxal in the beverage is 500-903 ppb, and the total content of bound CML, bound CEL, and bound MG-H1 in the beverage is 200 ppb or less, the binding type CML, the binding type CEL, and the binding type MG-H1 are included in a peptide component having a molecular weight of 400-3000 Da. The beer-flavor alcoholic beverage has an enhanced mellowness and a reduced astringency.