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Use of lotus procyanidin as advanced glycosylation end product formation inhibitor

A technology of advanced glycosylation and lotus root, applied in the field of medicine

Inactive Publication Date: 2014-01-15
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no report of lotus proanthocyanidins as an inhibitor of advanced glycation end products at home and abroad.

Method used

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  • Use of lotus procyanidin as advanced glycosylation end product formation inhibitor
  • Use of lotus procyanidin as advanced glycosylation end product formation inhibitor
  • Use of lotus procyanidin as advanced glycosylation end product formation inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] A pharmaceutical composition with lotus proanthocyanidins as the main active ingredient. Lotus proanthocyanidins account for 70 or 75 or 80%, synergists such as VE, VC, gallocatechin gallate (EGCG) and cysteine ​​and other compounds account for 10 or 15 or 20%, (select the above synergists One of the applications can be) other natural extracts such as lotus leaf extract and ginkgo leaf extract account for 5 or 8 or 10% by weight (the weight ratio of lotus leaf extract and ginkgo leaf extract is 1:1 ). Various capsules, Various health care products or food additives such as tablets or beverages can be made into tablets, capsules, granules, oral liquids, sustained-release preparations, controlled Release preparations, nano preparations, injections any pharmaceutically acceptable dosage form.

[0054] Inhibitory effect of lotus proanthocyanidins on the formation of advanced glycation end products under simulated physiological environment

[0055] 1 Experimental materia...

Embodiment 2

[0081] Example 2: In the simulated food system, the inhibitory effect of lotus proanthocyanidins on the formation of advanced glycation end products

[0082] 1 Experimental materials:

[0083] 1.1 Experimental raw materials: raw material preparation method as in Example 1

[0084] 1.2 Experimental reagents:

[0085] Disodium hydrogen phosphate, sodium dihydrogen phosphate, and α-lactose were all of analytical grade and were purchased from Sinopharm Chemical Reagent Co., Ltd.; L-Lysine (L-Lysine) biochemical reagent was purchased from BIOSHARP, Japan.

[0086] 1.3 Main instruments:

[0087] Shimadzu RF5301 fluorescence spectrophotometer, HH-2S constant temperature water bath.

[0088] 2 Experimental method:

[0089] 2.1 The inhibitory effect of LSPC on AGEs under different reaction times, determine the heating reaction time:

[0090] 2.1.1 Experimental method:

[0091] Accurately weigh 1.8016g of α-lactose and 0.731g of L-Lysine (the molar ratio of the substances is 1:1),...

Embodiment 3

[0105] Example 3: Discussion on the Inhibitory Mechanism of Lotus Proanthocyanidins on the Formation of Advanced Glycation End Products

[0106] 1 Experimental materials:

[0107] 1.1 Experimental raw materials: The raw material preparation method is the same as in Example 1.

[0108] 1.2 Experimental reagents:

[0109] Disodium hydrogen phosphate, sodium dihydrogen phosphate, methylglyoxal, and o-phenylenediamine were all analytically pure, purchased from Sinopharm Chemical Reagent Co., Ltd.; aminoguanidine (AG), 2,3-dimethylquinoxaline Catechin and catechin were analytically pure, purchased from sigma; methanol chromatographically pure, purchased from Fisher Scientific, USA.

[0110] 1.3 Main instruments:

[0111] Waters e2695 high performance liquid chromatography, Agilent 1100 high performance liquid chromatography-mass spectrometry.

[0112] 2 Experimental method:

[0113] 2.1 Scavenging effect of LSPC and its monomer Catechin on MGO

[0114] 2.1.1 Experimental meth...

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Abstract

The present invention discloses a use of lotus procyanidin as an advanced glycosylation end product formation inhibitor. The drug composition adopting the lotus procyanidin as a main active component comprises the following nature active component extracts, by weight, 70-80% of lotus procyanidin, 10-20% of a synergist such as VE, VC, epigallocatechin gallate (EGCG), cysteine and other ??complexes, and 5-10% of other nature extracts such as a lotus leaf extract, a ginkgo leaf extract and the like. According to the present invention, a high performance liquid chromatography method is adopted to detect a methylglyoxal removing effect of the lotus procyanidin and the main structure unit thereof (catechin), wherein methylglyoxal is an important intermediate substance during an advanced glycosylation end product formation process; a high performance liquid chromatography / multi-stage mass spectrometry method is adopted to identify adducts of four catechin and methylglyoxal and adducts of nine lotus procyanidin and methylglyoxal; and results show that lotus procyanidin provides good inhibition effects for advanced glycosylation end product formation in simulated physiological environments and simulated food systems.

Description

technical field [0001] The invention relates to the field of medicine, and more specifically relates to the application of a lotus proanthocyanidin in the preparation of natural medicine (inhibitor) for inhibiting the formation of advanced glycation end products in health food and food additives. Background technique [0002] Advanced glycosylation end products (AGEs) are formed by irreversible reactions between reducing sugars and proteins or lipids in the human body, and are the final products of the non-enzymatic glycosylation aging theory. Without the participation of enzymes, the aldehyde group or ketone group of the reducing sugar is combined with the amino group of the protein amino acid through nucleophilic combination, and the unstable Schiff base is first generated, and the Schiff base undergoes chemical rearrangement to form a relatively stable sugar compound Amadori product. This is the early stage of the glycosylation reaction. Amadori products or a variety of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L1/30A23L1/315A23L1/314A23L1/217A23C9/152A23F5/00A23L13/40A23L13/50A23L19/18
Inventor 孙智达吴茜李书艺关亚飞谢笔钧
Owner HUAZHONG AGRI UNIV
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