461 results about "Anthocyanidin" patented technology

The invention discloses a preparation method and an application of an intelligent label for judging the critical freshness of pork, and belongs to the field of fresh food fresh-keeping. The preparation method comprises the following steps: adding an anthocyanidin solution into a mixed solution of methyl cellulose and polyethylene glycol-6000 under stirring; removing bubbles in the solution, casting the solution on a glass plate, drying the solution by hot air, then peeling off the film namely an indicator layer; and wrapping the indicator layer by a low density polyethylene membrane to obtainthe intelligent label. The intelligent label can be adhered in a package, and the freshness of pork can be monitored in real time according to the color change of the intelligent label. The intelligent label can be used as an indicator card for rapidly detecting the freshness of pork. A certain amount of pork and the intelligent label are placed in a sealed box / bag, and the freshness of the pork can be judged according to the color change of the intelligent label. A natural pigment is taken as the indicator, the intelligent label does not contact with the pork, the safety is high, the manufacturing technology is simple, the freshness of pork can be further detected by an electronic tongue, and the results are reliable.

An extractant for extracting proanthocyanidin from plant is metaphosphoric acid and / or sulfite. Its extracting process includes adding water to prepare solution, regulating pH value, immersing extracting by controlling temp and time, filtering, purifying, solvent eluting, concentrating and drying. Its advantages are dosage of extractant, high extracting rate and high purity and water solubility of product.

A process is disclosed for the production of polyphenol oligomers having n polyphenol monomers, n being an integer from 2-18. The process includes coupling of a protected polyphenol, having protected phenolic hydroxyl groups, with a C-4 functionalized polyphenol monomer. The protected polyphenol may be a protected polyphenol monomer or a protected polyphenol oligomer having 2-17 monomers. Advantageously, polyphenol monomers forming the polyphenol oligomers may be the same or different.

The invention provides a purple corn beverage and a preparation method thereof. The major raw materials of the beverage are purple corn seeds and purple corn byproducts, fruit and vegetable pulp and flavorings are added to the beverage, and the beverage is treated by enzymolysis and acylation, so that cyanidin in the raw material purple corn is effectively extracted and stably stored in the beverage, thereby obtaining a purple corn beverage with rich cyanidin and unique taste.The scheme of the invention realizes the overall utilization of the purple corn plants; the cyanidin provides the function of health care, and the fruits, vegetables and flavorings not only feature the unique mouthfeel, but also boast various nutrient contents; and thus, the purple corn beverage is a novel health care beverage.

The invention provides a preparation method of cyaniding-3-O-glucoside chloride. Specifically, the method comprises the steps of: subjecting purple crops as the raw materials to an extraction with an extract of ethanol water solution with a volume fraction of 30-80% and a pH value of 1-4, leaving the obtained anthocyanidin crude extract to an ultrafiltration treatment through an ultrafilter membrane with a molecular weight cut-off of 1000-5000 D, and freeze drying the filtrate so as to obtain filtrate dry powder with a molecular weight less than 1000-5000 D, preparing a column-loading solution of 0.05-5wt% with the filtrate dry powder and water, purifying the filtrate dry powder preliminarily on a gel chromatographic column, conducting elution and collecting the eluent located at a position of 520nm and with an absorption peak, and reserving fractions containing cyaniding-3-O-glucoside chloride; purifying the fractions containing cyaniding-3-O-glucoside chloride further with preparative HPLC (high performance liquid chromatography), and collecting cyaniding-3-O-glucoside chloride absorption peaks. According to the invention, by improving the conditions and operations of extraction separation and purification, sugar, protein, other types of anthocyanin and flavonoid pigments in extracts can be effectively removed. Meanwhile, with simple steps and safe reagents, the method of the invention is beneficial for obtaining high purity cyaniding-3-O-glucoside chloride products.

Provided herein are methods and compositions for treating or preventing metabolic disorders, such as obesity and diabetes. Methods may comprise modulating the activity or level of a sirtuin, such as SIRTl or Sir2. Exemplary methods comprise contacting a cell with a sirtuin activating compound, such as a flavone, stilbene, flavanone, isoflavone, catechin, chalcone, tannin or anthocyanidin, or an inhibitory compound, such as nicotinamide.

Pharmaceutical compositions containing a proanthocyanidin polymer composition which are useful for the treatment and prevention of secretory diarrhea are provided. The invention specifically relates to pharmaceutical formulations of a proanthocyanidin polymer composition which has been isolated from a Croton spp. or a Calophyllum spp. In particular, the invention relates to a formulation of a proanthocyanidin polymer composition which protects the composition from the effects of stomach acid after oral administration, particularly to those formulations which are enteric coated. The invention also relates to methods of producing a directly compressible proanthocyanidin polymer composition, as well as compositions containing the directly compressible proanthocyanidin polymer composition.

The invention discloses a making method to crack C-C bond among protocyanin polymer into oligomer protocyanin, which comprises the following steps: dissolving polymer in the solvent; adding 1-10mg / ml degrading liquid in the polymer; adding 5-20% acid catalyst in the degrading liquid; swaying evenly; reacting under 40-90 deg.c for 0.5-2h; insulating air.

Procyanidin, optionally combined with hydroxycitric acid, is used in a method for the reduction of appetite in a mammal. Preferably procyanidin is administered to the mammal in a dosage of between 0.5 and 100 mg per kg bodyweight. Also shown is a process for the manufacture of an appetite reducing composition.

The present invention relates to the production of malic polyphenol as one polyphenol mixture with apple peel slag. Apple peel pomace is used as material in extracting malic polyphenol mixture through the technological processes of extraction, adsorption, separation, spray drying or decompression drying. The malic polyphenol mixture consists of catechin, epicatechin, phloretin and proanthocyanin,has several physiological activity and may be used in antioxidant, antimutagent and anomalism resisting medicine. Using apple peel pomace to replace apple in producing malic polyphenol can reduce production cost.

The invention discloses the preparation method of carotene, which comprises the following steps: (1) cleaning purple carrot and slicing; (2) extracting and filtering; (3) column chromatography; (4) concentrating and ultra-filtering; (5) micro-encapsulating; (6) spray-drying; and (7) sieving and mixing. The invention also discloses a carotene product, which is purple black powder with a color value equal to or larger than 50 and no peculiar carrot smell, and the carotene product is rich in cyanidin, has resistance to light and heat, and is insensitive to the pH value. Compared with the prior art, the invention has the advantage that the carotene preparation method is provided on the premise that ensures product style and internal quality stability and meets the requirement of consumers and has simple process, mild process condition, short preparation cycle, less equipment investment, recycle use of waste water and reuse of waste as feed additives, and the method is suitable for continuous and large-scale industrial production. The carotene of purple carrot has the advantages of low cost, stable quality and high reliability.

The invention discloses a preparation method of proanthocyanidin. In the method, grape seed, pine bark, purple potato or any other plant with high proanthocyanidin content is used as a raw material, an ethanol aqueous solution is used as an extraction solvent, and a double water phase extraction method is utilized to efficiently prepare proanthocyanidin from a crude proanthocyanidin extract. The invention has the advantages of high product yield, high product purity, low solvent consumption, low cost and the like, and can easily implement industrial production.

This invention relates to methods and compositions for preventing and treating skin rash, such as perineal dermatitis, associated with enzymatic dermatitis. More particularly, this invention relates to compounds containing procyanidins, which possess trypsin and / or chymotrypsin inhibitory activity and are suitable for use in compositions for preventing and treating skin irritation caused by protease exposure, such as perineal dermatitis.

The invention discloses a method for separating and preparing five high-purity anthocyanidin monomers from grape skins, relating to the preparation of natural pigments and belonging to the fields of food safety and food analytic chemistry. The method comprises the following steps of: firstly, carrying out anthocyanidin digestion on carbernet sauvignon grape skins to obtain crude extractive of anthocyanidin; then purifying the crude extractive by using XAD-7HP macroporous resin, and removing most of moisture absorption components such as saccharides, inorganic salts, phlegmatic temperament and the like in the crude extractive; and carrying out preparative liquid phase separation on the extractive of anthocyanidin subjected to the preliminary purification to obtain the high-purity monomers of the anthocyanidin. The method has the advantages of only using alcohol and acid as a solvent in stages of extraction, purification and separation in the preparation of the anthocyanidin and being environment-friendly, simple, convenient and feasible. The five anthocyanidin monomers obtained after purification are respectively delphinidin glucoside, 3'-methyl delphinidin glucoside, dimethyl delphinidin glucoside, dimethyl delphinidin acetylation glucoside, and dimethyl delphinidin trans-tonka-bean acetylation glucoside, the purities of which are sequentially 99.9%, 99.9%, 99.9%, 99.9%, 91.7%, 95.5%.

The present invention relates to the extraction of proanthocyanidin from plant material, and is especially the resin extraction process of proanthocyanidin from grape seed. Grape seed as material is extracted with water and / or alcohol as solvent at normal temperature or heating condition; the extractive liquid is filtered, concentrated while recovering alcohol solvent, re-filtered while adding water, the filtrate is adsorbed with macroporous adsorptive resin, the saturated resin is eluted with water solution of alcohol and the eluent is dried while recovering solvent to obtain the product. The process has yield of 3-10 %, and the product has proanthocyanidin content of 38-90 %. The present invention has the advantages of low cost and no residual solvent in the product, and is environment friendly and suitable for industrial production.

The invention discloses a process for hypercritical extraction of grape seed oil and resin adsorption abstraction of proanthocyanidin, which comprises, employing hypercritical carbon dioxide to extract grape seed oil from comminuted grape seeds under abstraction pressure of 20-35MPa at 40-50 deg. C, heating degreased grape seeds with 25-35% water solution of ethanol for backflow, charging 50-70% ethanol or isopropanol solution into the concentrated extract liquid, agitating and stewing, decompressing and filtering, thus obtaining proanthocyanidin crude extract liquid, then adsorbing with polystyrene resin, carrying out desorption with desorbent after adsorption saturation, drying the eluent under reduced pressure, finally obtaining refined proanthocyanidin product.

The invention discloses a method for separating and extracting ipomoes batatas L. anthocyanidins, which comprises the following steps: cleaning, pulping and crushing the fresh ipomoes batatas L.; then, putting the crushed ipomoes batatas L. pulp into aqueous solution of acid ethanol, and extracting the solution with the aid of ultrasonic wave; filtering, compressing and concentrating the extract,adsorbing the extract by adopting NKA, washing the impurities by water, and eluting the extract by the aqueous solution of acid ethanol; and concentrating and drying the elution to obtain an ipomoes batatas L. anthocyanidins product. The method of the invention is characterized in that: ultrasonic wave-aid extraction and NKA separation and purification are adopted so as to ensure the efficient extraction of the anthocyanidins in the ipomoes batatas L. and ideal separation and purification effects and lay a foundation for the utilization and industrialized development of the ipomoes batatas L.resource.

A method is disclosed that prevents, controls and ameliorates urinary tract infections caused by E. coli by administering a therapeutically effective amount of a human dietary supplement composition comprising a cranberry derivative or proanthocyanidin containing concentrate and D-mannose combined in a form suitable for oral consumption. The cranberry derivative or proanthocyanidin containing concentrate comprises from about one percent (1.0%) by weight to about 95 percent (95.0%) by weight on a dry weight basis. The composition is formulated for delivering a D-mannose unit dosage between about 0.5 to about 5.0 grams per dose and further comprising an analgesic or antispasmodic or combination thereof, and optionally a diuretic, and wherein the composition formed from the cranberry derivative or proanthocyanidin containing concentrate, D-mannose are effective together for binding to E. coli to aid in flushing the E. coli from the urinary tract system while preventing binding of E. coli to cells in the urinary tract system.

The invention discloses a method for extracting and comprehensively utilizing anthocyanidin from Ipomoes batatas. The method comprises the following steps: cleaning the Ipomoes batatas, immersing, fragmenting and mashing the Ipomoes batatas under weak acid and normal temperature conditions, separating batata slag, naturally precipitating, taking an upper layer aqueous solution of anthocyanidin, filtering twice and carrying out membrane filtration, and adsorbing, eluting and concentrating a filtrate to obtain a pasty anthocyanidin product; 2, processing the pasty anthocyanidin product with a routine method to obtain a solid anthocyanidin product; 3, washing precipitated and filtered starch with water, and processing with a routine method to obtain a starch product; 4, carrying out mother solution processing which comprises controlling the pH value of the mother solution at 4.0-4.2 and controlling the pH value of the mother solution at 4.4-4.5 after filtering, sending an upper solution to a fragmentation and mashing operation, carrying out reduced pressure concentration on a lower layer sol, and processing with a routine method to obtain a protein product and a mucopolysaccharide product; and 5, recovering separated water, a separated mother solution or a separated organic solvent, and processing waste residues with a routine method to obtain a forage and a fertilizer. The method of the invention has the advantages of chemical reagent and water consumption reduction, product yield improvement, comprehensive utilization of the Ipomoes batatas, added value improvement, and good social benefit and environmental protection benefit.

The invention relates to a treatment method for enhancing stability of blueberry cyanidin. The treatment method comprises the following steps: extracting with a 0.5% trifluoroacetic acid (TFA) methanol solution, and sequentially purifying through an ion exchange resin column Amberlite XAD-7 and a Sephadex column Sephadex LH-20 to obtain a blueberry cyanidin refined substance; carrying out nutgall acylation reaction on the blueberry cyanidin refined substance and prepared triacetyl nutgall acyl chloride to introduce galloyl group with ortho-triphenolhydroxy group into the molecular structure, thereby obtaining the modified product nutgall acylated cyanidin. When high-performance liquid chromatography is used for determining the gallic acid amount generated after hydrolyzing the modified product, the nutgall acylation degree is 55-63%. The experiment proves that the stability of the modified product is obviously enhanced. The method for treating blueberry cyanidin is simple to operate, and has the advantages low cost, low pollution and high acylation degree. The molecular modification can enhance the primary characteristics of the blueberry cyanidin, so that the effects are enhanced; and multiple functional groups are introduced to endow the blueberry cyanidin with new physiological activity, thereby widening the application range.

The invention discloses a method for extracting ipomoes batatas L. anthocyanidins, which comprises the following steps: baking the ipomoes batatas L. by microwave, then placing the baked ipomoes batatas L. into acid electrolysis water, and repeatedly homogenizing and extracting, filtering, separating and drying the ipomoes batatas L. to prepare anthocyanidins powder. The method for extracting theanthocyanidins has the advantages of high efficiency, simple process, low cost and environmental protection; the obtained ipomoes batatas L. anthocyanidins extract has fresh and stable color; and thecontent of active ingredients such as total flavonoids, total phenols, total sugars and total monomer anthocyanidins and the like in the extract is higher. The ipomoes batatas L. anthocyanidins extract prepared by the method has stronger reducing power, capacity of eliminating free radicals and function of lipid peroxidation resistance in vitro, has quite obvious anti-oxidation effect in vivo, and can remarkably delay the ageing of the organism. The ipomoes batatas L. anthocyanidins extract can be widely used in the fields of food colorant, anti-ageing health-care food and the like.

The invention discloses a method for preparing proanthocyanidins liposomes, and relates to a method for preparing liposomes. The method solves the problems of large grain size and difficult absorption of a human body by protecting proanthocyanidins in a microcapsule form in the prior art. The method comprises the following steps of: 1, putting soya bean lecithin and cholesterol in a pear-shape flask, adding mixed solution of diethyl ether and absolute ethyl alcohol, adding proanthocyanidins and phosphate buffer, and mixing to obtain mixed solution; 2, performing ultrasonic processing on the mixed solution in a 25 DEG C water bath, performing rotary evaporation to form a film in a water bath at the temperature of between 30 and 50 DEG C and under the pressure of between 400 and 700MPa, adding phosphate buffer again, and performing hydration to obtain coarse suspension; and 3, and filtering the coarse suspension by using a filter membrane of 0.45 mu m. The liposomes are adopted for encapsulating the proanthocyanidins, so the proanthocyanidins are more stable, have small grain size and target, and are favorable for absorption of the human body; the encapsulation rate of the proanthocyanidins liposomes is 88.89 percent; and the preparation process is simple.

The invention discloses a blueberry and waxy corn juice and a processing method thereof. The juice comprises, by weight, 18-30 parts of blueberry, 8-15 parts of pyracantha fortuneana fruit, 50-80 parts of purplish black waxy corn, 6-10 parts of durian pit, 10-20 parts of a dark plum juice, 2-3 parts of lychee seed, 2-3 parts of white gourd peel, 1-2 parts of hawthorn seed, 2-4 parts of Helianthus tuberosus, 1-3 parts of pueraria flower, 1-2 parts of Chinese fevervine herb, 2-3 parts of persimmon leaf, 1-2 parts of Lagotis Gaertn, 6-12 parts of a food additive, 15-25 parts of rice wine, and a proper amount of water. The adoption of blueberry and purplish black waxy corn rich in phenol compounds and anthocyanidins in the invention improves the body anti-oxidation function, and the synergistic effect of blueberry, purplish black waxy corn and other many traditional Chinese medicinal extract products makes the juice remove the advantages of removal of body toxins, reduction and stabilization of the blood pressure, blood sugar balance recovery, comprehensive recovery of the body functions and human body health benefiting after frequent drinking.

The invention provides a grape seed proanthocyanidins health beverage and a preparation method. The beverage is prepared by the following steps of: extracting leftover grape seeds in a wine plant, performing combined membrane separation technology on the extract to obtain clarified, deastringented and proanthocyanidins-enriched purified liquid, and adding water and various flavoring agents into the purified liquid in a ratio to obtain the product. The beverage does not have the astringent taste of the grape seed per se and yeast taste generated in the wine fermentation process, and also fully keeps the medicinal values of the grape seed.

The invention relates to the technical field of extraction of effective parts of plants, in particular to an extraction and separation method of flavone and cyanidin from elderberry fruits. The invention aims to overcome the problems of complicated raw material treatment, low recovery and content of target constituents of products, and poor product color in the prior art. The provided technical proposal is as follows: the extraction and separation method of the flavone and cyanidin from the elderberry fruits comprises the following steps: first, extraction: crushing the raw materials, adding to a container for evenly stirring, dipping and filtering to obtain clear leach solution of the elderberry fruits for use; second, performing adsorption and elution by a macroporous resin adsorption column to obtain a condensed extract; and third, drying the extract to obtain a finished product. Compared with the prior art, the method has the advantages of 1. simple raw material treatment, 2. high recovery and content of target constituents of the product, 3. good color (mauve), and 4. simple process.

The invention relates to a method for extracting anthocyanidin by using a compound enzyme, and a compound enzyme preparation thereof. The compound enzyme preparation disclosed by the invention comprises the following components of 7-15 wt% of cellulase, 7-15 wt% of hemicellulase, 7-15 wt% of a protease and 55-79% of pectinase. The method for extracting anthocyanidin by using the compound enzyme comprises the following steps of (1) preparing fresh fruit pulp, and adjusting a solid liquid ratio with water; (2) stirring the fruit pulp obtained from the steps (1) in a sealed container at a low speed, controlling a temperature within 40-60 DEG C and a pH value within 3-6, adding the above compound enzyme preparation, and enzymatic hydrolyzing for 1-8 hours, wherein the usage amount of the compound enzyme is 1 mg / g-3 mg / g; (3) removing fruit residues from the fruit pulp after enzymatic hydrolysis is finished to obtain clear fruit juice; and (4) refining the clear fruit juice by chromatography, and spray-drying to obtain an anthocyanidin product.

A method for extracting and separating procyanidine comprises the steps of: separating the coat from the kernel of grape seeds or seabuckthorn seeds; crushing the seed coat into 10 meshes to 80 meshes; adding the crushed seed coat into alkali-water solution, alkali-alcohol-water solution or alkali-acetone-water solution with the pH value of 9 to 14 according to the solid-liquid ratio of 1g:1-10ml, conducting extraction for 1 hour to 10 hours at the temperature of 10 DEG C to 50 DEG C and then filtration; repeatedly extracting and filtering a filter cake until the filtrate is colorless or pale color; merging the filtrate and concentrating the filtrate till the weight of the filtrate is 0.5 time to 2 times of the weight of the seed coat raw materials; extracting the concentrated liquid with organic solvent and separating the liquid for obtaining an organic phase and a water phase; neutralizing the water phase with acid and adding methanol or ethanol with the volume of 1 time to 10 times, stirring, standing and filtrating; evaporating the filtrate, washing the obtained solid with water and then drying the solid for obtaining an oligomeric procyanidine product; and washing the filter cake with water and then drying the filter cake for obtaining the high-polymerization procyanidine. The invention has the advantages of less raw material input quantity, high product purity, high extraction rate, green environmental protection, simple process, and convenient industrial production.

The present invention has an object to provide an agent for strengthening calcium-containing tissues, which can be safely applied; and its use: The present invention solves the object by providing an agent for strengthening calcium-containing tissues, which comprises one or more flavones, flavonols, flavanones, flavanonols, anthocyanidins, flavanols, chalcones, and aurones.

The invention provides a clean and health-care wet tissue comprising anthocyanidin. The anthocyanidin health wet tissue can eliminate skin surface radicals, has functions for improving body blood circulation, killing bacteria, resisting aging, improving immunity and beautifying the skin and is health-care. The wet tissue is mild, and does not irritate the skin, and has high safety. The wet tissue comprises the following raw materials in percentage by weight: a disinfecting and cleaning solution containing 0.3-3.0% of anthocyanidin, 0.01-0.5% of preservative and 0.5-6.0% of wetting agent, and a dry tissue made of a superior non-woven fabric or paper tissue. The product solves the problems that the common wet tissue on the market has irritation to the skin and damages the skin. The wet tissue belongs to the field of a health daily article, and has high popularization value.

The invention relates to a method for extracting proanthocyanidin from cranberryin, comprising: treating material to obtain cranberryin extracting liquid; performing degreasing; and separating and purifying by using column chromatography. The method of the invention is simple in process, low in cost, easy in industrialization.