30 results about "Anthocyanidin" patented technology

The application relates to the technical field of polypeptide preparation, and particularly discloses a preparation method of animal blood globin polypeptide powder. The preparation method of the animal blood globin polypeptide powder comprises the following steps: adding trisodium citrate into fresh animal blood, stirring for 10-30 min, adding cyanidin and polylysine, stirring uniformly, placing in a temperature-10 to-20 DEG C environment for 1-2 h, increasing to room temperature, homogenizing for 1-5 min, centrifuging, removing supernatant, and obtaining animal blood globin solution; adding the animal blood globin solution and rare earth lanthanum chitosan amine chelate salt into a glycine-sodium hydroxide buffer solution, stirring at 40-50 DEG C for 10-30 min, adding compound protease, and continuing to stir for 10-20 h to obtain an enzymatic solution; adjusting the pH value of the enzymatic solution to 5-6, increasing the temperature to 90-100 DEG C for 5-10 min, decreasing to room temperature, centrifuging for 5-10 min, and taking supernatant for low-temperature spray drying.

The invention relates to the field of separation and extraction of active ingredients of natural products, in particular to a comprehensive separation process of various active ingredients in peanut coats. Through reasonable matching of all the steps, various peanut coat active ingredients including peanut coat total sterol, peanut coat saturated fatty acid, peanut coat unsaturated fatty acid, peanut coat total flavonoid compounds and peanut coat procyanidine can be obtained with high yield through a set of process, the yield is high, and the method is a method capable of effectively and comprehensively utilizing peanut coat resources and improving the production efficiency. And the method is suitable for industrial technological processes.

To provide a simple procyanidin analysis method that can be performed at a low cost, and in the form of preliminary analysis for selecting a sample for precision analysis in HPLC, that is, screening.SOLUTION: Cell size liposomes 10, 10' are composed of a lipid component system in which one or more phospholipids 2, 3 and one or more sterol-based lipids 5 are used in a constant composition, and procyanidin 8, where different phase separation domain generation patterns are generated depending on the concentration of procyanidin 8, which can be used for estimating the procyanidin concentration from the phase separation domain generation pattern. The phospholipids 2, 3 are unsaturated phospholipid or saturated phospholipid.SELECTED DRAWING: Figure 1

The invention discloses health wine capable of delaying senescence as well as a preparation method and application of the health wine, and belongs to the technical field of health food. According to the health-preserving wine, mulberries and sea-buckthorn are used as monarch materials, rhizoma polygonati and radix puerariae are used as ministerial materials, lophatherum gracile, fructus lycii and Chinese yam are used as assistants and guides, and all the raw materials are medicinal and edible substances. According to the invention, through an optimized'warm soaking-cold soaking 'two-step extraction process, synergistic interaction is realized, and active ingredients such as anthocyanin, flavone, polysaccharide and puerarin are extracted to the greatest extent. The health-preserving wine disclosed by the invention not only has good antioxidant capacity, but also can be used for remarkably removing free radicals such as DPPH (1, 1-diphenyl-2-picrylhydrazyl) and hydroxyl, improving the activity of serum SOD (superoxide dismutase) and GSH-Px (glutathione The formation of advanced glycosylation end products can be effectively inhibited, and the obvious anti-aging and anti-saccharification dual effects are achieved. The health-preserving wine disclosed by the invention is simple in preparation process, mellow in taste and suitable for daily health-preserving drinking.

The invention relates to the technical field of coreopsis tinctoria refined components and preparation and application, and provides a coreopsis tinctoria refined component and a preparation method and application thereof.The coreopsis tinctoria refined component comprises, by mass, 11 specific components of phenolic acids, flavonol, flavanol and flavonoid, and the 11 specific components comprise, by mass, 2 parts of gallic acid, 1 part of potassium sorbate, 1 part of potassium sorbate, 1 part of potassium sorbate and 1 part of potassium sorbate. The health-care food is prepared from the following raw materials in parts by weight: 3 parts of chlorogenic acid, 14 parts of quercetin, 18 parts of catechin, 5 parts of citric acid, 2-3 parts of procyanidine B, 3 parts of (+)-epicatechin, 1-31 parts of 31 parts of procyanidine C, 14 parts of rutin, 4 parts of camphanol-3-O-rutinoside and 2 parts of isorhamnetin-3-O-rutinoside. The snow chrysanthemum is Changbai Mountain snow chrysanthemum. Animal experiments show that the refined component can significantly reduce the content of total cholesterol and triglyceride in serum of a high-fat model rat, the specific component with blood fat regulating activity is prepared from Changbai Mountain snow chrysanthemum, and a new choice is provided for non-drug intervention of dyslipidemia.

A nanomaterial is gold or silver nanoparticle having a nanoparticle surface that consists of one or more of the following extracts from sweet gum leaves: shikimic acid, sweet gum leaf family of Catechins, sweet gum leaf family of Quercetin, Sweroside, Afzelechin, p-Coumaric Acid, Epiafzelechin Trimethyl Ether, Myricetin, Naringenin, Phloretin, Procyanidin B1 and related polyphenols, flavonoids and alkaloids. An antiviral / antibacterial agent includes sweet gum mediated silver nanoparticles having a core size and in a concentration having efficacy as an antiviral / antibacterial exceeding a 3 log 10 reduction in viral titer.

The invention discloses a compound melanterite pill specific chromatogram and a construction method and a quality detection method thereof, and belongs to the field of Chinese patent medicine detection. Taking different batches of compound melanterite pills as test articles to prepare a compound melanterite pill test article solution; the method comprises the following steps: taking gallic acid, ginsenoside Rg1, procyanidine B2, cinnamic acid, coumarin, ginsenoside Rh1, cinnamyl alcohol, cinnamaldehyde, arachidonic acid, epicatechin, 2-methoxycinnamic acid, ginsenoside Rb1, ginsenoside Rd, betulinic acid and 5-hydroxymethylfurfural as reference substances to prepare a single reference substance solution; performing chromatographic analysis on the compound melanterite pill test solution and the single reference substance solution, and recording chromatograms; carrying out similarity analysis; and constructing the characteristic chromatogram of the compound melanterite pill according to the chromatogram of the test solution of the compound melanterite pill and the chromatogram of the single reference substance solution in combination with the total ion chromatogram obtained by the high-resolution mass spectrum and the mass spectrum result map of the chemical components. The method can accurately, clearly and objectively evaluate the quality of the compound melanterite pill.

The invention relates to the technical field of nano material imaging diagnosis, in particular to a lipid droplet detection kit based on double-response water-soluble fluorescent dye and application of the lipid droplet detection kit. The invention provides the lipid droplet detection kit, the lipid droplet detection kit comprises a component A, the component A is a composite fluorescent dye of an anthocyanin derivative fluorescent dye and a metal frame material, the dual response of polarity and RNS can be realized, the lipid droplet imaging effect is good, the dyeing step is simple, the water solubility is better, the lipid droplet is easy to rinse and clean, the experimental result is reliable, and the lipid droplet detection kit has great commercial value.

The invention belongs to the technical field of biological medicine, and particularly relates to a bionic hybrid membrane coating baicalein-loaded procyanidine nano preparation as well as a preparation method and application thereof. The procyanidine nano preparation is prepared from procyanidine, baicalein, an erythrocyte membrane, a microglial cell membrane, an RVG targeting peptide and an MG1 targeting peptide. Baicalein is entrapped in nanoparticles PC (at) BAI formed by self-assembly of procyanidine, a bionic hybrid membrane is used as an outer layer to coat the surface of the nanoparticles PC (at) BAI to obtain a nano material M (at) PC (at) BAI, and an RVG targeting peptide and an MG1 targeting peptide are modified on the surface of the nano material M (at) PC (at) BAI to obtain the nano preparation RVG / MG1-M (at) PC (at) BAI. The biomimetic hybrid membrane coating is combined with the biological function of a natural cell membrane and the stability of synthetic lipid, so that the nano preparation is endowed with excellent long-acting blood circulation capacity and active targeting capacity to a brain lesion area, and the enrichment of the drug in the brain is remarkably improved.

The present application relates to the technical field of biological medicine, and specifically provides a visual hydrogel based on multiple response mechanisms and a preparation method and application thereof.The preparation method comprises the following steps: (1) preparing an inclusion compound by using beta-cyclodextrin and hydroxypropyl-beta-cyclodextrin and cyanidin; (2) respectively preparing a polyvinyl alcohol / rhein solution and a mixed solution of quaternary ammonium salt chitosan / hyaluronic acid / κ-carrageenan; (3) initiating dynamic crosslinking by using phenylboronic acid, and constructing a multiple network structure in cooperation with tannic acid, the inclusion compound and genipin, and then curing the hydrogel through gradient temperature control.The hydrogel has pH / temperature / ROS response characteristics, and can use the color change of cyanidin to feed back the wound microenvironment state in real time, and meanwhile, hyaluronic acid / polyvinyl alcohol can construct a 3D microchannel structure, promote the ordered arrangement of collagen, and accelerate wound healing, thereby solving the problems of unstable direct loading of cyanidin and difficulty of a single response mechanism in adapting to the complex microenvironment of a diabetic wound.

The invention discloses injectable hierarchical controlled-release double-drug-loading hydrogel coupled with MOF and a preparation method of the injectable hierarchical controlled-release double-drug-loading hydrogel. The injectable hierarchical controlled-release double-drug-loading hydrogel can be used for combined delivery of drugs including procyanidine B2 and melatonin. The drug PC2 is used as a crosslinking point, the hydrophobic drug MT is encapsulated in the MOF, and the hydrophilic drug PC2 is used as a bridge to connect the MOF and the hydrogel, so that slow and controllable release of the drug is realized, and injectability and self-repairing performance of the hydrogel are maintained. And finally, efficient sequential delivery of the procyanidine B2 and the melatonin in the intervertebral disc is realized.

The invention discloses an additive for enhancing physical ability for plateau hypoxia as well as a preparation method and application of the additive, and belongs to the technical field of biological medicines. The invention relates to an auxiliary agent for enhancing physical ability due to plateau hypoxia. The auxiliary agent is formed by wrapping a flavonoid compound and a carrier, the molecular weight of a flavonoid compound in the auxiliary agent is not higher than 1500Da; the carrier in the auxiliary agent is a pharmaceutically acceptable carrier. The invention also provides a preparation method and application of the auxiliary agent. Based on a procyanidine core and a synergistic anti-oxidation carrier, the aid for enhancing physical ability due to plateau hypoxia can inhibit reduction of myocardial cell mitochondrial membrane potential caused by hypoxia and reduce physical ability loss caused by hypoxia.

The invention relates to the technical field of antioxidant products, in particular to a composition for resisting oxidation and relieving chronic inflammation, a product and application. The composition for resisting oxidation and relieving chronic inflammation comprises a first antioxidant, a second antioxidant and a third antioxidant, the first antioxidant comprises sheep lung peptide and amino acid; the second antioxidant comprises B vitamins and a yeast extract; the third antioxidant comprises vitamin C, vitamin E, trace elements and components containing bioactive substances; the bioactive substances at least comprise phenols and flavonoids, the phenols at least comprise procyanidine, the flavonoids at least comprise dihydromyricetin, and the components containing the bioactive substances at least comprise a grape seed extract and an ampelopsis grossedentata extract; the procyanidine content in the grape seed extract is greater than or equal to 95%, and the dihydromyricetin content in the ampelopsis grossedentata extract is greater than or equal to 40%. The composition disclosed by the invention has the advantages of better anti-oxidation effect and chronic inflammation relieving effect.

The invention provides a synthesis method of procyanidine B3. Specifically, the invention provides a preparation method of a compound 6, which comprises the following steps: in a solvent, under the action of a catalyst, a compound 4 and a compound 5 are subjected to a condensation reaction to obtain the compound 6, the catalyst is (R) 3SiOTf, and R is independently C1-4 alkyl or phenyl. The preparation method provided by the invention is low in cost, simple and convenient to operate and good in industrial application prospect.

The invention relates to hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biological hydrogel materials. The hydrogel comprises a prednisolone sodium phosphate hydrogel P-Gel skeleton and gold nanoparticles PCAu, wherein the gold nanoparticles PCAu are uniformly loaded on the P-Gel skeleton and are coated by procyanidine. According to the preparation method, a three-dimensional cross-linked network skeleton of PSP hydrogel P-Gel is self-assembled and constructed by utilizing ion coordination and hydrogen-bond interaction of prednisolone sodium phosphate PSP under the action of Ca < 2 + >, and the hydrogel PCAu-coated P-Gel is formed by uniformly loading gold nanoparticles PCAu which are green synthesized from procyanidine PC and have stable surfaces. The hydrogel can promote rapid repair of spinal cord injury and relieve far-end muscle atrophy and fibrosis; and meanwhile, the injectable self-healing hydrogel has the engineering advantages of injectability, self-healing and the like, and shows good clinical transformation potential.

The invention discloses a human Fc receptor composite sealant for flow cytometry as well as a preparation method and application thereof, and belongs to the technical field of biology. The composite sealant is composed of thiophosphorylated oligodeoxynucleotide, human serum and ProClin 300, the thiophosphorylated oligodeoxynucleotide is obtained by carrying out thiophosphorylation modification on three deoxynucleotides at each of two ends of an oligodeoxynucleotide sequence, and the nucleotide sequence of the thiophosphorylated oligodeoxynucleotide is as shown in SEQ ID No.1. The composite sealing agent can effectively seal non-specific binding of a human Fc receptor and an anthocyanin fluorescent dye labeled antibody, and does not affect specific binding of the antibody and a target antigen. The composite sealing agent provided by the invention has the advantages of synergistic interaction, high stability, simplicity and convenience in operation and the like, and is suitable for multicolor flow cytometry analysis.

The invention discloses a calanthe japonica flower color and flower fragrance component analysis method, and belongs to the technical field of plant component analysis. The method comprises the following steps: S1, synchronous collection and pretreatment of samples: collecting flower buds and flowers of the same scapes, respectively taking petals and lips, and uniformly dividing the petals and lips into flower fragrance and pigment detection samples; s2, targeted extraction and GC-MS (gas chromatography-mass spectrometry) detection of flower fragrance components: extracting by adopting headspace solid-phase microextraction combined with an internal standard method, and performing GC-MS analysis by optimizing a heating program; s3, high-efficiency extraction and HPLC (High Performance Liquid Chromatography) detection of the anthocyanidin: carrying out ultrasonic extraction by adopting a composite solvent containing vitamin C, and carrying out HPLC analysis through gradient elution; and S4, data processing and dynamic correlation analysis: performing qualitative and quantitative analysis on a detection result. Through targeted sampling and composite extraction technologies, the problems of high sample consumption, long period and lack of data relevance in a traditional method are solved, accurate analysis of the flower color and flower fragrance components of the calanthe plants is realized, and a key technical support is provided for gardening breeding.

This invention relates to the field of natural product separation and purification technology, specifically to a proanthocyanidin C1 extraction system and method. The aim is to solve the problems of high spectral overlap between proanthocyanidin C1 and its homomeric isomers, overlapping peak edges, and the inability of traditional fixed elution flow rates to adapt to real-time micro-changes in peak shape, leading to inaccurate fraction collection and low extraction purity and yield. In liquid chromatography detection, a real-time spectral micro-shift data capture unit extracts the shoulder peak spectral micro-shift values ​​within a narrow wavelength range at the edge of the characteristic absorption peak. A micro-shoulder peak shift correction algorithm unit fits the instantaneous correlation coefficient between the shift value and the elution flow rate using the least squares method, generating a flow rate adjustment command. A dynamic elution flow rate adjustment unit drives the elution pump to adjust the flow rate, establishing a dynamic coupling relationship. The fraction collection node calculates compensation for the shift and locks the characteristic peak position of the pure component, precisely controlling the start and end times of collection, thereby improving the extraction purity and yield of proanthocyanidin C1.

The invention provides a composition for repairing tooth enamel damage and a preparation method and application thereof, and the composition for repairing tooth enamel damage comprises the following raw materials: glaze protein, procyanidine, a calcium ion source, a phosphate ion source and an orally acceptable carrier. According to the composition provided by the invention, by combining the glaze protein, the procyanidine and a biological remineralization technology, mineralized crystals can be induced to directionally grow along the damaged hydroxyapatite surface, a firmer structure is formed, remineralization is sustainable, and enamel is helped to be better repaired.

The invention discloses a preparation method of polysaccharide-based functional hydrogel constructed based on multi-component reaction, and belongs to the field of biomedical materials. The method comprises the following steps: preparing dialdehyde cellulose, preparing a chitosan solution, preparing a dialdehyde cellulose-anthocyanin solution and finally forming the hydrogel. Cellulose is oxidized by sodium periodate to prepare dialdehyde cellulose, and the dialdehyde cellulose, a chitosan solution and anthocyanin are subjected to multi-component reaction under mild conditions to form a covalent cross-linked network in situ. According to the invention, the use of toxic chemical cross-linking agents is avoided, the process is simple, the conditions are mild, and the prepared hydrogel has injectability, self-healing property, good swelling property and excellent antibacterial and antioxidant activity and blood compatibility, and is suitable for the biomedical fields of wound dressing, drug delivery and the like.

To provide a simple procyanidin analyzing method capable of being performed at a low cost and having a form such as preliminary analysis or screening for selecting a sample to be subjected to precise analysis by HPLC (high performance liquid chromatography).SOLUTION: The cell-sized liposome 110 is a cell-sized liposome composed of a lipid component system using one or more kinds of saturated phospholipids 13 in addition to one or more kinds of unsaturated phospholipids 12, and a sample or procyanidin 18 of a procyanidin concentration estimation object, and is used for procyanidin concentration estimation from a phase separation domain generation pattern by generating a different phase separation domain generation pattern by the concentration of the procyanidin 18. The lipid component system of the cell-sized liposome 110 may include a sterol-based lipid such as cholesterol. Further, a calibration curve can be used for concentration estimation.SELECTED DRAWING: Figure 1-3

The application provides a bio-based Mannich base, a preparation method thereof, an application thereof, and a TDI curing agent prepared by using the bio-based Mannich base as a catalyst. The bio-based Mannich base is obtained by a Mannich reaction of natural phenol, dimethylamine and formaldehyde, wherein the natural phenol is one or more selected from the group consisting of sesamol, catechin, theaflavin, daidzein and procyanidin. The Mannich base catalyst prepared by using the natural phenol in the application improves steric hindrance, and the reaction selectivity of small molecule monomers can be improved through the steric hindrance effect. The content of free TDI monomers in the TDI curing agent can be reduced by adding the catalyst at one time, so that the free monomers in the prepared TDI curing agent are less than 0.5% advantageously.

The invention discloses a sweet taste enhancing and taste improving composition based on procyanidine B2 and cyanidin-3-O-glucoside, and the composition is prepared from the following raw materials in parts by weight: 0.05 to 0.07 part of aspartame or 0.01 to 0.03 part of sucralose, 0.02 to 0.03 part of cyanidin-3-O-glucoside, 0.02 to 0.03 part of procyanidine B2, 1.5 to 2.5 parts of erythritol and the balance of water. According to the invention, a double-polyphenol synergistic sweetening system is innovatively constructed, a sweetness signal is enhanced by cyanidin-3-O-glucoside, a sweetening agent is stabilized by procyanidine B2, sweetness perception is prolonged, bad aftertaste is neutralized by matching with erythritol, mellow feeling is supplemented, and an integrated scheme of double-polyphenol sweetening and sugar alcohol regulation is formed. Compared with theoretical superposition, the composition has the advantages that the sweetness is improved by 45.6%, the synergistic increasing rate reaches 28.9%, bad flavors such as metal flavor and bitter taste are remarkably inhibited, the aftertaste residue is less than or equal to 2s, the taste is close to that of cane sugar, and the composition can be widely applied to low-sugar food, takes low sugar and health into account, and has important industrial application value.

An example of a composition for promoting metabolic health can include a combination of cyanidin and delphinidin in an amount sufficient to treat metabolic disorders.Furthermore, an example of a method for treating a condition or disorder related to metabolic health in a subject can include reducing endotoxin levels compared to baseline endotoxin levels, and adjusting cardiometabolic biomarkers related to lipid or glucose metabolism from abnormal cardiometabolic baseline levels to normal levels.Exemplary methods can also include reducing liver inflammation, treating high-fat diet-induced colon dysfunction, reducing the expression of toll-like receptors TLR4 and TLR2, reducing plasma endotoxin levels, reducing the expression of NADPH oxidase NOX1, and increasing tight junction proteins in the colon of a subject.

The invention discloses a Qingcheng tea high-dihydroxy catechin related molecular marker based on a liquid phase chip and application thereof, and belongs to the technical field of biology. According to the invention, a liquid phase chip developed at an early stage and a large amount of transcriptome data are utilized; 17 SNP mutation sites distributed on coding genes of flavonoid 3 '5' hydroxylase, flavonoid 3 'hydroxylase, achromocyanidin reductase and dihydroxyflavonol reductase of the Xiangcheng tea are identified, and the mutation sites can obviously influence the expression of related genes, so that the distribution proportion of dihydroxy catechin and trihydroxy catechin of the Xiangcheng tea is regulated and controlled; and according to the genotype characteristics, the high-dihydroxy catechin Guangcheng tea germplasm resources can be rapidly screened and identified. The method fills the technical blank of accurate, efficient and low-cost identification of high-dihydroxyl defencheng tea resources, and also provides a theoretical basis for researching a high-dihydroxyl catechin molecular mechanism and the like.

The present invention relates to a liquid tea product. More particularly the present invention relates to a ready-to-drink liquid tea product. Accordingly, a liquid tea product comprising: a. a tea extract; and, b. a compound comprising a flavylium cation; wherein the total polyphenol content in the product is less than 0.04% by weight, and further wherein i) the structure of the flavylium cation is: formula (I); ii) the compound comprising flavylium cation belongs to the group of anthocyanins; and, iii) the pH of the product is less than 6.

To provide a composition that can improve or maintain vascular functions, or in particular, can improve or maintain vascular functions, hematologic properties, a blood flow and the like.SOLUTION: The present invention provides a composition containing procyanidin, and ceramide or nucleic acids.SELECTED DRAWING: Figure 1

The application provides a detection method for gelatin concentration in electrolyte for copper electrolytic refining, comprising: forming a precipitate by reacting the filtered copper electrolytic refining electrolyte with a cyanidin solution; dissolving the precipitate with a precipitate dissolving agent and then reacting with a chromogenic agent; determining the strongest absorption wavelength U and its absorbance value by using an ultraviolet-visible spectrophotometer; preparing a control solution, and obtaining a net absorbance value by background deduction; preparing 5-10 standard copper solutions with known gelatin concentrations, and determining the net absorbance value of each standard copper solution; and calculating the gelatin concentration in the electrolyte according to the gelatin concentration and the net absorbance value of the standard copper solution fitting a standard exponential equation curve. The combined process of specific precipitation separation-dissolution-color development reaction-ultraviolet spectrophotometric detection realizes accurate determination of the gelatin concentration in the complex copper electrolyte system, and has the technical effects of strong anti-interference, simple operation, wide detection range, high precision and low equipment dependency.

The invention discloses a cranberry mousse type private care solution and a preparation method thereof.According to the care solution, cranberry extract rich in A-type proanthocyanidins and cordate houttuynia extract rich in flavonoids serve as core active ingredients, an amino acid type surfactant and a moisturizing and adjusting component are compounded, and a homogeneous dispersion system with the pH value being 4.3-5.5 is constructed. The preparation method comprises the following steps: inhibiting the enzyme activity of raw materials in a low-temperature acid environment, locking heat-sensitive components through 35 DEG C supercritical extraction, and forming a micron-sized micelle structure by using a 20-30MPa high-pressure homogeneous shear force induction system. When the finished product is pumped out through a mousse pump, micron-sized foam with the particle size being smaller than or equal to 0.1 mm is formed, and the touch feeling is soft and moist without friction; through detection, the retention rate of the type A proanthocyanidins is greater than or equal to 98.7%, the bacteriostasis rate on common pathogenic bacteria is greater than or equal to 99.96%, and through triple authoritative safety verification of eye stimulation, skin sensitization and vagina mucosa stimulation, the composition has the synergistic effects of mild cleaning, efficient bacteriostasis and micro-ecological balance maintenance.