47 results about "Anthocyanidin" patented technology

The invention provides a ginseng exosome anti-aging and anti-oxidation composition and a preparation method thereof, and relates to the field of nursing materials, the ginseng exosome anti-aging and anti-oxidation composition comprises Exo nano-vesicles, procyanidine and cationic liposome, the Exo nano-vesicles are derived from ginseng extracellular fluid, the procyanidine is derived from the cationic liposome, and the cationic liposome is derived from the procyanidine. The ginseng exosome anti-aging and anti-oxidation composition provided by the invention can effectively improve skin wrinkles, enhance skin elasticity, promote collagen synthesis, inhibit collagen degradation and inhibit elastin degradation through the combined action of the Exo nano-vesicles, Pro and cationic lipidosome; degradation of elastin can cause skin to lose resilience, formation of wrinkles is accelerated, and facial skin can be effectively improved and wrinkles can be reduced by promoting synthesis of collagen and inhibiting degradation of elastin.

The invention relates to the technical field of 3D printing of easy-to-swallow food, in particular to a protein-based nanoparticle gel double-nozzle 3D printing method and equipment, aiming at the nutritional requirements of old people with dysphagia, procyanidine is loaded on protein-based nanoparticles, corn starch, gelatin and the like are combined to construct a composite gel system as a printing wall material, and the printing wall material is used as a printing wall material. Protein, citrus juice and peony seed oil emulsion are used as core materials, and a double-nozzle 3D printing technology is utilized to realize accurate texture regulation and control and personalized nutrition design; the method comprises the steps of protein solution preparation, composite nanoparticle synthesis, procyanidine loading, wall material and core material preparation, double-nozzle printing and the like, the technology breaks through the bottleneck that traditional swallowing food is insufficient in protein strengthening and lacks active ingredients, and the antioxidant functional components and the 3D printing technology are integrated; innovative food with swallowing safety and nutrition targeting is provided for the elderly, and application of protein-based materials in intelligent food manufacturing is promoted.

The invention relates to the field of cell culture and discloses a culture medium for thick-shelled mussel mantle cells and an application thereof. The culture medium comprises a basal culture medium and additives, wherein the additives comprise thick-shelled mussel serum, proanthocyanidins, bovine insulin, growth factors, antibiotics, defensins, mussel mucin and a buffer. The culture medium can be used for in vitro culture of thick-shelled mussel mantle cells and can better maintain the survival and growth of in vitro cultured thick-shelled mussel mantle cells. Defensins in a specific proportion are added to the culture medium, and the best growth and reproduction state can be achieved when the thick-shelled mussel mantle cells are cultured using the culture medium in the specific proportion. The application also provides an application method of the culture medium, which is simple to operate and can produce cultured thick-shelled mussel mantle cells with high cell density, good morphology and high wall adhesion.

The invention discloses a honeysuckle flower pigment synthesis gene and application thereof in regulating and controlling the content and resistance of plant anthocyanidin, and belongs to the technical field of gene engineering. The nucleotide sequence of the honeysuckle flower pigment synthesis gene is as shown in SEQ ID NO. 6. The invention provides a honeysuckle flower pigment synthesis gene. The gene has the effects of regulating and controlling the content of anthocyanidin in plants and regulating and controlling the stress resistance of the plants. The gene is introduced into a plant body by constructing an overexpression vector, so that the gene is stably overexpressed, the anthocyanidin content in the plant is remarkably increased, and the petal color and seed coat color of the plant are darkened; the resistance of the plant to drought stress and salt stress is also remarkably enhanced, so that the plant can adapt to a severe environment more easily, and a new gene resource and a theoretical support are provided for breeding of high-resistance plant varieties.

The invention discloses an intestinal tract hemostatic composition as well as a preparation method and application thereof, and relates to the field of hemostatic drugs. Wherein the catechin, the hyperoside, the isoquercitrin, the quercetin-3-O-glucuronide, the gallocatechin, the myricetin-3-O-galactoside, the proanthocyanidin B1, the proanthocyanidin B3, the syringe-3-O-glucoside and the astragalus smicus glycoside are flavonoid compounds; nuciferine, higenamine, N-methyl-higenamine and N-trans-feruloyl tyramine are taken as alkaloid compounds, and the compound is selected from the group consisting of higenamine, nuciferine, higenamine The composition has the advantages of simple preparation process, good hemostatic effect and the like, and provides a new raw material choice for the development of intestinal bleeding treatment medicines.

The application relates to the technical field of polypeptide preparation, and particularly discloses a preparation method of animal blood globin polypeptide powder. The preparation method of the animal blood globin polypeptide powder comprises the following steps: adding trisodium citrate into fresh animal blood, stirring for 10-30 min, adding cyanidin and polylysine, stirring uniformly, placing in a temperature-10 to-20 DEG C environment for 1-2 h, increasing to room temperature, homogenizing for 1-5 min, centrifuging, removing supernatant, and obtaining animal blood globin solution; adding the animal blood globin solution and rare earth lanthanum chitosan amine chelate salt into a glycine-sodium hydroxide buffer solution, stirring at 40-50 DEG C for 10-30 min, adding compound protease, and continuing to stir for 10-20 h to obtain an enzymatic solution; adjusting the pH value of the enzymatic solution to 5-6, increasing the temperature to 90-100 DEG C for 5-10 min, decreasing to room temperature, centrifuging for 5-10 min, and taking supernatant for low-temperature spray drying.

The invention relates to the field of separation and extraction of active ingredients of natural products, in particular to a comprehensive separation process of various active ingredients in peanut coats. Through reasonable matching of all the steps, various peanut coat active ingredients including peanut coat total sterol, peanut coat saturated fatty acid, peanut coat unsaturated fatty acid, peanut coat total flavonoid compounds and peanut coat procyanidine can be obtained with high yield through a set of process, the yield is high, and the method is a method capable of effectively and comprehensively utilizing peanut coat resources and improving the production efficiency. And the method is suitable for industrial technological processes.

To provide a simple procyanidin analysis method that can be performed at a low cost, and in the form of preliminary analysis for selecting a sample for precision analysis in HPLC, that is, screening.SOLUTION: Cell size liposomes 10, 10' are composed of a lipid component system in which one or more phospholipids 2, 3 and one or more sterol-based lipids 5 are used in a constant composition, and procyanidin 8, where different phase separation domain generation patterns are generated depending on the concentration of procyanidin 8, which can be used for estimating the procyanidin concentration from the phase separation domain generation pattern. The phospholipids 2, 3 are unsaturated phospholipid or saturated phospholipid.SELECTED DRAWING: Figure 1

The invention discloses health wine capable of delaying senescence as well as a preparation method and application of the health wine, and belongs to the technical field of health food. According to the health-preserving wine, mulberries and sea-buckthorn are used as monarch materials, rhizoma polygonati and radix puerariae are used as ministerial materials, lophatherum gracile, fructus lycii and Chinese yam are used as assistants and guides, and all the raw materials are medicinal and edible substances. According to the invention, through an optimized'warm soaking-cold soaking 'two-step extraction process, synergistic interaction is realized, and active ingredients such as anthocyanin, flavone, polysaccharide and puerarin are extracted to the greatest extent. The health-preserving wine disclosed by the invention not only has good antioxidant capacity, but also can be used for remarkably removing free radicals such as DPPH (1, 1-diphenyl-2-picrylhydrazyl) and hydroxyl, improving the activity of serum SOD (superoxide dismutase) and GSH-Px (glutathione The formation of advanced glycosylation end products can be effectively inhibited, and the obvious anti-aging and anti-saccharification dual effects are achieved. The health-preserving wine disclosed by the invention is simple in preparation process, mellow in taste and suitable for daily health-preserving drinking.

The invention relates to the technical field of coreopsis tinctoria refined components and preparation and application, and provides a coreopsis tinctoria refined component and a preparation method and application thereof.The coreopsis tinctoria refined component comprises, by mass, 11 specific components of phenolic acids, flavonol, flavanol and flavonoid, and the 11 specific components comprise, by mass, 2 parts of gallic acid, 1 part of potassium sorbate, 1 part of potassium sorbate, 1 part of potassium sorbate and 1 part of potassium sorbate. The health-care food is prepared from the following raw materials in parts by weight: 3 parts of chlorogenic acid, 14 parts of quercetin, 18 parts of catechin, 5 parts of citric acid, 2-3 parts of procyanidine B, 3 parts of (+)-epicatechin, 1-31 parts of 31 parts of procyanidine C, 14 parts of rutin, 4 parts of camphanol-3-O-rutinoside and 2 parts of isorhamnetin-3-O-rutinoside. The snow chrysanthemum is Changbai Mountain snow chrysanthemum. Animal experiments show that the refined component can significantly reduce the content of total cholesterol and triglyceride in serum of a high-fat model rat, the specific component with blood fat regulating activity is prepared from Changbai Mountain snow chrysanthemum, and a new choice is provided for non-drug intervention of dyslipidemia.

A nanomaterial is gold or silver nanoparticle having a nanoparticle surface that consists of one or more of the following extracts from sweet gum leaves: shikimic acid, sweet gum leaf family of Catechins, sweet gum leaf family of Quercetin, Sweroside, Afzelechin, p-Coumaric Acid, Epiafzelechin Trimethyl Ether, Myricetin, Naringenin, Phloretin, Procyanidin B1 and related polyphenols, flavonoids and alkaloids. An antiviral / antibacterial agent includes sweet gum mediated silver nanoparticles having a core size and in a concentration having efficacy as an antiviral / antibacterial exceeding a 3 log 10 reduction in viral titer.

The present application relates to the technical field of photonic neural network, and discloses a fluorescent photonic neural network, a construction method thereof and an image recognition system.The construction method comprises the following steps: providing a single-layer photochromic spiropyran film; using a neural network based on a Keras framework to perform simulation training to obtain a weight matrix of a to-be-identified task; writing the weight matrix of the to-be-identified task into the single-layer photochromic spiropyran film, so that the concentration distribution of cyanine on the single-layer photochromic spiropyran film conforms to the weight matrix, and the fluorescent photonic neural network is obtained; and the fluorescent photonic neural network uses the spontaneous fluorescence of the spiropyran film as the signal output of the to-be-identified task.The present application can realize the storage of the computer fitting weight by the spiropyran film by using the mutual conversion of spiropyran and cyanine under the action of ultraviolet light and visible light, and can automatically sense ultraviolet signals and visible light signals.

The invention relates to the technical field of medicines, in particular to a stem cell-loaded drug-loaded nano-vesicle-sericin-based hydrogel and application thereof. According to the preparation method, sericin is taken as a base material, sericin is chemically grafted through methacrylic anhydride to obtain photo-crosslinked sericin (SerMA), then procyanidine (PC) loaded acrylate-polyethylene glycol-succinimide ester (AC-PEG-NHS) modified cell membrane nano-vesicles are grafted to a molecular skeleton of the cell membrane nano-vesicles through photo-crosslinking reaction, and the SerMA-PC-coated PNVs hydrogel is successfully prepared. In-vitro and in-vivo experimental studies of the hydrogel show that the SerMA-PC-coated PNVs / PDLSCs hydrogel can regulate the polarization state of microglial cells through oxidation resistance, so that the survival of damaged retinal ganglion cells (RGCs) is promoted, and the SerMA-PC-coated PNVs / PDLSCs hydrogel can be used for treating eye diseases such as glaucoma.

The invention discloses a compound melanterite pill specific chromatogram and a construction method and a quality detection method thereof, and belongs to the field of Chinese patent medicine detection. Taking different batches of compound melanterite pills as test articles to prepare a compound melanterite pill test article solution; the method comprises the following steps: taking gallic acid, ginsenoside Rg1, procyanidine B2, cinnamic acid, coumarin, ginsenoside Rh1, cinnamyl alcohol, cinnamaldehyde, arachidonic acid, epicatechin, 2-methoxycinnamic acid, ginsenoside Rb1, ginsenoside Rd, betulinic acid and 5-hydroxymethylfurfural as reference substances to prepare a single reference substance solution; performing chromatographic analysis on the compound melanterite pill test solution and the single reference substance solution, and recording chromatograms; carrying out similarity analysis; and constructing the characteristic chromatogram of the compound melanterite pill according to the chromatogram of the test solution of the compound melanterite pill and the chromatogram of the single reference substance solution in combination with the total ion chromatogram obtained by the high-resolution mass spectrum and the mass spectrum result map of the chemical components. The method can accurately, clearly and objectively evaluate the quality of the compound melanterite pill.

The invention relates to the technical field of nano material imaging diagnosis, in particular to a lipid droplet detection kit based on double-response water-soluble fluorescent dye and application of the lipid droplet detection kit. The invention provides the lipid droplet detection kit, the lipid droplet detection kit comprises a component A, the component A is a composite fluorescent dye of an anthocyanin derivative fluorescent dye and a metal frame material, the dual response of polarity and RNS can be realized, the lipid droplet imaging effect is good, the dyeing step is simple, the water solubility is better, the lipid droplet is easy to rinse and clean, the experimental result is reliable, and the lipid droplet detection kit has great commercial value.

The invention belongs to the technical field of biological medicine, and particularly relates to a bionic hybrid membrane coating baicalein-loaded procyanidine nano preparation as well as a preparation method and application thereof. The procyanidine nano preparation is prepared from procyanidine, baicalein, an erythrocyte membrane, a microglial cell membrane, an RVG targeting peptide and an MG1 targeting peptide. Baicalein is entrapped in nanoparticles PC (at) BAI formed by self-assembly of procyanidine, a bionic hybrid membrane is used as an outer layer to coat the surface of the nanoparticles PC (at) BAI to obtain a nano material M (at) PC (at) BAI, and an RVG targeting peptide and an MG1 targeting peptide are modified on the surface of the nano material M (at) PC (at) BAI to obtain the nano preparation RVG / MG1-M (at) PC (at) BAI. The biomimetic hybrid membrane coating is combined with the biological function of a natural cell membrane and the stability of synthetic lipid, so that the nano preparation is endowed with excellent long-acting blood circulation capacity and active targeting capacity to a brain lesion area, and the enrichment of the drug in the brain is remarkably improved.

The present application relates to the technical field of biological medicine, and specifically provides a visual hydrogel based on multiple response mechanisms and a preparation method and application thereof.The preparation method comprises the following steps: (1) preparing an inclusion compound by using beta-cyclodextrin and hydroxypropyl-beta-cyclodextrin and cyanidin; (2) respectively preparing a polyvinyl alcohol / rhein solution and a mixed solution of quaternary ammonium salt chitosan / hyaluronic acid / κ-carrageenan; (3) initiating dynamic crosslinking by using phenylboronic acid, and constructing a multiple network structure in cooperation with tannic acid, the inclusion compound and genipin, and then curing the hydrogel through gradient temperature control.The hydrogel has pH / temperature / ROS response characteristics, and can use the color change of cyanidin to feed back the wound microenvironment state in real time, and meanwhile, hyaluronic acid / polyvinyl alcohol can construct a 3D microchannel structure, promote the ordered arrangement of collagen, and accelerate wound healing, thereby solving the problems of unstable direct loading of cyanidin and difficulty of a single response mechanism in adapting to the complex microenvironment of a diabetic wound.

The invention belongs to the technical field of antiviral drugs, and in particular relates to a novel application of procyanidine A1 in preparation of a drug for inhibiting porcine epidemic diarrhea virus (PEDV). The porcine epidemic diarrhea (PED) is a high-contact intestinal infectious disease caused by the PEDV (porcine epidemic diarrhea virus), so that huge economic loss is caused to the global pig breeding industry. Currently, PEDV serving as an RNA virus is extremely easy to mutate, so that the protection effect of the existing vaccine is limited, and a special-effect antiviral therapeutic drug is lacked clinically. It is found for the first time that the natural compound procyanidine A1 has remarkable inhibitory activity on the porcine epidemic diarrhea virus and can effectively inhibit replication of the virus in cells. In-vitro experiments prove that the procyanidine A1 has no obvious toxicity to cells at an effective antiviral concentration and has high safety. The invention provides a brand new and effective drug choice for prevention and control of PEDV, and has important application value and wide market prospect.

The invention discloses injectable hierarchical controlled-release double-drug-loading hydrogel coupled with MOF and a preparation method of the injectable hierarchical controlled-release double-drug-loading hydrogel. The injectable hierarchical controlled-release double-drug-loading hydrogel can be used for combined delivery of drugs including procyanidine B2 and melatonin. The drug PC2 is used as a crosslinking point, the hydrophobic drug MT is encapsulated in the MOF, and the hydrophilic drug PC2 is used as a bridge to connect the MOF and the hydrogel, so that slow and controllable release of the drug is realized, and injectability and self-repairing performance of the hydrogel are maintained. And finally, efficient sequential delivery of the procyanidine B2 and the melatonin in the intervertebral disc is realized.

The invention discloses an additive for enhancing physical ability for plateau hypoxia as well as a preparation method and application of the additive, and belongs to the technical field of biological medicines. The invention relates to an auxiliary agent for enhancing physical ability due to plateau hypoxia. The auxiliary agent is formed by wrapping a flavonoid compound and a carrier, the molecular weight of a flavonoid compound in the auxiliary agent is not higher than 1500Da; the carrier in the auxiliary agent is a pharmaceutically acceptable carrier. The invention also provides a preparation method and application of the auxiliary agent. Based on a procyanidine core and a synergistic anti-oxidation carrier, the aid for enhancing physical ability due to plateau hypoxia can inhibit reduction of myocardial cell mitochondrial membrane potential caused by hypoxia and reduce physical ability loss caused by hypoxia.

The present invention discloses a method for establishing a UPLC fingerprint of Weimaining capsules, comprising the following steps: (1) preparing a test solution; (2) respectively examining the precision of an ultra-high performance liquid chromatograph, the repeatability of a test solution preparation method, and the stability of the test solution; (3) subjecting the test solution to gradient elution in a chromatographic column, and scanning the test solution using an ultra-high performance liquid chromatograph to obtain a chromatogram of the test solution; (4) identifying the common peak components of the chromatogram using reference substances such as gallic acid, protocatechuic acid, protocatechuic aldehyde, proanthocyanidin B2, epicatechin, and rutin. (5) establishing a fingerprint. The ultra-high performance liquid chromatograph used in the present invention has high precision, and the test solution preparation method provided has good repeatability. The fingerprint established based on the above two points has strong stability, and the speed of establishing the fingerprint is fast, the sensitivity is high, and the separation of the chromatographic peaks is good.

The present invention discloses a method for constructing a characteristic spectrum of golden buckwheat. The characteristic spectrum has gallic acid, protocatechuic acid, protocatechuic aldehyde, catechin, epicatechin, 4-coumaric acid, and procyanidin B2 as characteristic components. By using an efficient detection method, the common peaks of golden buckwheat samples of different origins are confirmed to construct a characteristic spectrum. The method can distinguish the counterfeit products of golden buckwheat, Smilax glabra and Smilax china, and with the flavonoids contained in golden buckwheat as representative components, it can more comprehensively reflect the product quality. The method is suitable for rapid and comprehensive quality control of golden buckwheat medicinal materials, decoction pieces, extracts, standard decoctions, formula granules, and benchmark samples, preparation intermediates, and finished products of golden buckwheat classic prescriptions. The method is efficient, easy to operate, and suitable for quality monitoring of continuous production.

The invention relates to the technical field of antioxidant products, in particular to a composition for resisting oxidation and relieving chronic inflammation, a product and application. The composition for resisting oxidation and relieving chronic inflammation comprises a first antioxidant, a second antioxidant and a third antioxidant, the first antioxidant comprises sheep lung peptide and amino acid; the second antioxidant comprises B vitamins and a yeast extract; the third antioxidant comprises vitamin C, vitamin E, trace elements and components containing bioactive substances; the bioactive substances at least comprise phenols and flavonoids, the phenols at least comprise procyanidine, the flavonoids at least comprise dihydromyricetin, and the components containing the bioactive substances at least comprise a grape seed extract and an ampelopsis grossedentata extract; the procyanidine content in the grape seed extract is greater than or equal to 95%, and the dihydromyricetin content in the ampelopsis grossedentata extract is greater than or equal to 40%. The composition disclosed by the invention has the advantages of better anti-oxidation effect and chronic inflammation relieving effect.

The application discloses a kind of nano composite hydrogel dressings with reversible adhesion-photothermal sterilization function and preparation method thereof.The preparation method includes the following:1) preparation of metal organic framework ZIF-8, which is immersed in a solution containing cyanidin CY-7 to achieve drug adsorption;DA is then used to coat the surface of the obtained sample to obtain ZIF-8 / CY-7 / PDA particles;2) preparation of 7-carboxymethoxyl-4-methyl coumarin CM with carboxyl at the end;3) through EDC coupling reaction, CM is grafted onto the side chain of dopamine-modified chitosan molecules to obtain CS-DA-CM;4) disperse ZIF-8 / CY-7 / PDA particles and angiogenic components in CS-DA-CM solution to obtain the dressing.The dressing can be injected and used, and can be gelled in situ to achieve adhesion when irradiated with a light source with a wavelength greater than 300 nm, and can be converted back to a solution when irradiated with a light source with a wavelength less than 280 nm;when an infrared wavelength with a wavelength of 820 nm is used, photothermal effect can be excited to achieve photothermal sterilization at the wound site, and photothermal effect can promote blood flow to accelerate wound healing.

The invention provides a synthesis method of procyanidine B3. Specifically, the invention provides a preparation method of a compound 6, which comprises the following steps: in a solvent, under the action of a catalyst, a compound 4 and a compound 5 are subjected to a condensation reaction to obtain the compound 6, the catalyst is (R) 3SiOTf, and R is independently C1-4 alkyl or phenyl. The preparation method provided by the invention is low in cost, simple and convenient to operate and good in industrial application prospect.

The invention relates to hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biological hydrogel materials. The hydrogel comprises a prednisolone sodium phosphate hydrogel P-Gel skeleton and gold nanoparticles PCAu, wherein the gold nanoparticles PCAu are uniformly loaded on the P-Gel skeleton and are coated by procyanidine. According to the preparation method, a three-dimensional cross-linked network skeleton of PSP hydrogel P-Gel is self-assembled and constructed by utilizing ion coordination and hydrogen-bond interaction of prednisolone sodium phosphate PSP under the action of Ca < 2 + >, and the hydrogel PCAu-coated P-Gel is formed by uniformly loading gold nanoparticles PCAu which are green synthesized from procyanidine PC and have stable surfaces. The hydrogel can promote rapid repair of spinal cord injury and relieve far-end muscle atrophy and fibrosis; and meanwhile, the injectable self-healing hydrogel has the engineering advantages of injectability, self-healing and the like, and shows good clinical transformation potential.

The invention discloses a human Fc receptor composite sealant for flow cytometry as well as a preparation method and application thereof, and belongs to the technical field of biology. The composite sealant is composed of thiophosphorylated oligodeoxynucleotide, human serum and ProClin 300, the thiophosphorylated oligodeoxynucleotide is obtained by carrying out thiophosphorylation modification on three deoxynucleotides at each of two ends of an oligodeoxynucleotide sequence, and the nucleotide sequence of the thiophosphorylated oligodeoxynucleotide is as shown in SEQ ID No.1. The composite sealing agent can effectively seal non-specific binding of a human Fc receptor and an anthocyanin fluorescent dye labeled antibody, and does not affect specific binding of the antibody and a target antigen. The composite sealing agent provided by the invention has the advantages of synergistic interaction, high stability, simplicity and convenience in operation and the like, and is suitable for multicolor flow cytometry analysis.

The invention discloses a duckweed LtF3 'H gene and application thereof. Relates to the technical field of gene engineering. The invention provides a gene sequence, an amplification primer and application. The key function of the F3 'H gene in plant secondary metabolites is that F3' H can catalyze naringenin to generate eriodictyol, and eriodictyol can influence accumulation of anthocyanidin, so that the content of cyanidin-3-o-glucoside in a duckweed LtF3 'H overexpression strain is remarkably increased, meanwhile, F3' H can catalyze apigenin to synthesize luteolin, and the yield of luteolin is increased. The luteolin is a mother nucleus of orientin and isoorientin, so that the contents of orientin and isoorientin in an overexpression strain of the duckweed LtF3 'H are remarkably increased, and meanwhile, the content of apigenin C-glycoside (vitexin and isovitexin) is reduced.

The invention discloses a calanthe japonica flower color and flower fragrance component analysis method, and belongs to the technical field of plant component analysis. The method comprises the following steps: S1, synchronous collection and pretreatment of samples: collecting flower buds and flowers of the same scapes, respectively taking petals and lips, and uniformly dividing the petals and lips into flower fragrance and pigment detection samples; s2, targeted extraction and GC-MS (gas chromatography-mass spectrometry) detection of flower fragrance components: extracting by adopting headspace solid-phase microextraction combined with an internal standard method, and performing GC-MS analysis by optimizing a heating program; s3, high-efficiency extraction and HPLC (High Performance Liquid Chromatography) detection of the anthocyanidin: carrying out ultrasonic extraction by adopting a composite solvent containing vitamin C, and carrying out HPLC analysis through gradient elution; and S4, data processing and dynamic correlation analysis: performing qualitative and quantitative analysis on a detection result. Through targeted sampling and composite extraction technologies, the problems of high sample consumption, long period and lack of data relevance in a traditional method are solved, accurate analysis of the flower color and flower fragrance components of the calanthe plants is realized, and a key technical support is provided for gardening breeding.

This invention relates to the field of natural product separation and purification technology, specifically to a proanthocyanidin C1 extraction system and method. The aim is to solve the problems of high spectral overlap between proanthocyanidin C1 and its homomeric isomers, overlapping peak edges, and the inability of traditional fixed elution flow rates to adapt to real-time micro-changes in peak shape, leading to inaccurate fraction collection and low extraction purity and yield. In liquid chromatography detection, a real-time spectral micro-shift data capture unit extracts the shoulder peak spectral micro-shift values ​​within a narrow wavelength range at the edge of the characteristic absorption peak. A micro-shoulder peak shift correction algorithm unit fits the instantaneous correlation coefficient between the shift value and the elution flow rate using the least squares method, generating a flow rate adjustment command. A dynamic elution flow rate adjustment unit drives the elution pump to adjust the flow rate, establishing a dynamic coupling relationship. The fraction collection node calculates compensation for the shift and locks the characteristic peak position of the pure component, precisely controlling the start and end times of collection, thereby improving the extraction purity and yield of proanthocyanidin C1.