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Method of preparing oligomeric proanthocyanidins

A technology of proanthocyanidins and oligomers, which is applied in the field of preparation of oligomer proanthocyanidins, can solve the problems of uneconomical production, instability, and easy decomposition, and achieve the effects of increased production, low production cost, and high antioxidant activity

Inactive Publication Date: 2007-08-08
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But the concentration that this method adopts is the hydrogen peroxide of 20-60% as degradative oxidant, unstable in use and storage process, easy to decompose, thereby causes catalyst efficiency to be low, consumes bigger; Adopt potassium chlorate to be oxidant and there is catalyst consumption too , while the cost of potassium chlorate is higher, making the production uneconomical
In addition, there are few technical reports in this area

Method used

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  • Method of preparing oligomeric proanthocyanidins

Examples

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Comparison scheme
Effect test

Embodiment 1

[0019] Preparation of proanthocyanidin high-polymerization extract: weigh 250.0 g of grape seeds, crush them, pass through a 20-mesh sieve, and place them in a 100-ml beaker. Add 250ml of petroleum ether to soak for 24 hours, filter, and soak the residue three times with new petroleum ether. Add 300ml of 60% (v / v) ethanol solution to the grape seed powder after degreasing, extract at 30° for 30min, extract three times, wash the final residue twice, collect the extract and washing solution, distill under reduced pressure at ≤ 37°C, and concentrate to The specific gravity of the solution is between 1.1-1.2, and 350ml of concentrated solution is obtained. Add 200ml of ethanol to the concentrated solution to remove impurities such as protein and polysaccharide by precipitation, and repeat three times. The filtrate was distilled and concentrated under reduced pressure until the specific gravity of the solution was between 1.0-1.05 to obtain 300 ml of a concentrated solution. Add ...

Embodiment 2

[0022] Take 10ml of the proanthocyanidin high-polymerization extract prepared in Example 1, add 0.4ml of glacial acetic acid, shake well, and react in a constant temperature water bath at 60°C for 30min. The average degree of polymerization of the hydrolyzate was 3.78 by sampling analysis.

Embodiment 3

[0024] Take 10ml of the proanthocyanidin high-polymerization extract prepared in Example 1, add 0.2ml of formic acid, shake well, and react in a constant temperature water bath at 50°C for 40min. The average degree of polymerization of the hydrolyzate was 4.47 in sampling analysis.

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Abstract

The invention discloses a making method to crack C-C bond among protocyanin polymer into oligomer protocyanin, which comprises the following steps: dissolving polymer in the solvent; adding 1-10mg / ml degrading liquid in the polymer; adding 5-20% acid catalyst in the degrading liquid; swaying evenly; reacting under 40-90 deg.c for 0.5-2h; insulating air.

Description

technical field [0001] This patent relates to a preparation method of oligomeric proanthocyanidins, in particular to a method for preparing oligomeric proanthocyanidins by degrading high-polymer proanthocyanidins under acidic conditions. Background technique [0002] Proanthocyanidin (proanthocyanidin) is a general term for flavan-3-ol or flavan-3,4-ol derivatives widely present in plants, and its structural formula is as follows (n represents the average degree of polymerization): [0003] [0004] Bagchi D, Garg A, Krohn R L, Bagchi M, Tran M X, Stohs S J. et al. in 1997, 95 (2) period 179-189 pages "Oxygen free radical scavenging abilities ofvitamins C and vitamins E, and a grape seed Proanthocyanidins extract in vitroresearch, Communications in Molecular Pathology and Pharmacology "shows that oligomer proanthocyanidins are the strongest antioxidants for scavenging free radicals (about 50 times that of natural vitamin E in the body), and it can prevent and treat more t...

Claims

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Application Information

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IPC IPC(8): C07D311/62C07D311/60
Inventor 任其龙魏作君苏宝根杨亦文
Owner ZHEJIANG UNIV
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