Method for extracting a great variety of biological active ingredients from dry powder of pasania fungus

A technology for biologically active ingredients and dry powder of shiitake mushrooms, which is applied in the fields of food science, application, food preparation, etc., can solve the problems of less research and development of biologically active ingredients of shiitake mushrooms, low polysaccharide yield, and increased extraction cost.

Inactive Publication Date: 2009-04-29
HENAN INST OF SCI & TECH
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Problems solved by technology

[0002] Among the bioactive ingredients contained in shiitake mushrooms, there are many research reports on lentinan at home and abroad, and the development and utilization are relatively in-depth, while the research and development of other bioactive ingredients in shiitake mushrooms is less
Moreover, the existing methods for extracting biologically active components of Lentinus edodes are mainly the extraction of a single component, such as Lentinan, Lentinan, etc., and there are no reports on the comprehensive extraction of multiple biologically active components.
Therefore, in the prior art, when extracting and preparing single components such as lentinan, other active ingredients in the mushrooms are discarded in vain, which not only leads to higher extraction costs, but also wastes the resources of the mushrooms
For example, as far as the extraction of lentinan is concerned, the hot water extraction method is commonly used at present. This...

Method used

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Embodiment

[0009] The first step is to extract alcohol solubles

[0010] Put 1 kg of shiitake mushroom dry powder into the reflux extraction device, then add about 5 kg of ethanol, the ethanol concentration is 85%-90%, heat and reflux at 80-90°C for 2 hours, remove the extract, and perform suction filtration, and filter the residue according to the above method. Extract once, remove the extract, perform suction filtration, combine the two filtrates, evaporate under reduced pressure at about 40°C, recover ethanol and concentrate the filtrate, and when concentrated to quick dry, transfer the concentrate and dry under vacuum at 40°C , to obtain 211.6 grams of alcohol-soluble matter, and the filter residue was used for the next step of extraction;

[0011] The second step is to extract the mushroom protein

[0012] Add 10 kg of sodium hydroxide aqueous solution with a pH value of 10 to the residue after extracting alcohol solubles, stir and extract at 4-20°C for 30 minutes, remove the extra...

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Abstract

The invention discloses a method for comprehensively extracting various kinds of biologically active ingredients from mushroom dry powder; the technical proposal is as follows: firstly, extracting alcohol soluble substances; secondly, extracting mushroom proteins; thirdly, extracting water soluble polysaccharide of mushroom, fourthly, extracting alkali extraction water-insoluble polysaccharide and alkali extraction water soluble polysaccharide; fifthly, preparing mushroom fiber. The extracted constituents have different biological activities, for example, the alcohol soluble substances have strong antioxidation activity; the mushroom proteins are rich in essential amino acid; the lentinan has antineoplastic activity; and the mushroom fiber has the physiological function of a dietary fiber. Therefore, the extracted constituents can be used respectively.

Description

Technical field: [0001] The invention relates to a method for comprehensively extracting multiple bioactive components from dry mushroom powder. Background technique: [0002] Among the bioactive ingredients contained in shiitake mushrooms, there are many research reports on lentinan at home and abroad, and the development and utilization are relatively in-depth, while the research and development of other bioactive ingredients in shiitake mushrooms is less. Moreover, the existing methods for extracting biologically active components of Lentinus edodes are mainly the extraction of a single component, such as Lentinan and Purine of Lentinus edodes, and there is no report on the comprehensive extraction of multiple biologically active components. Therefore, in the prior art, when extracting and preparing single ingredients such as lentinan, other active ingredients in the mushrooms are discarded in vain, which not only leads to higher extraction costs, but also wastes the reso...

Claims

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Application Information

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IPC IPC(8): A23L1/28A23L1/30A23L1/09A23L1/305A23L1/308A23J1/00A23L31/00A23L33/105A23L33/125A23L33/185A23L33/21
Inventor 李波师玉忠孙俊良芦菲索晓敏
Owner HENAN INST OF SCI & TECH
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