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A barrier film for guiding periodontal tissue regeneration and its preparation method and application

A technology for periodontal tissue regeneration and barrier membrane, applied in the field of biomedical materials, can solve the problems of weak cell adsorption, easy swelling, deformation, etc., and achieve the effect of good biocompatibility, mild reaction system and simple operation steps

Active Publication Date: 2019-04-30
WUHAN INST OF BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, a single material will have some shortcomings: the water resistance of the material prepared by SPI is poor, and it is easy to swell, deform and lose mechanical strength when it encounters water. These shortcomings can be overcome by modification treatment; while chitosan is easy to dissolve in a weak acid environment and loss, its microstructure, mechanical properties and degradation properties are poorly regulated, its cell affinity is not enough, and its cell adsorption is weak, so it needs further modification.

Method used

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  • A barrier film for guiding periodontal tissue regeneration and its preparation method and application
  • A barrier film for guiding periodontal tissue regeneration and its preparation method and application
  • A barrier film for guiding periodontal tissue regeneration and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 guides the preparation of periodontal tissue regeneration barrier membrane

[0032] (1) Preparation of soybean protein solution: Weigh 5g of soybean protein and dissolve it in 45g of distilled water, add dropwise 45g of 5% NaOH solution, stir evenly, add 25g of distilled water, and bathe in 50°C water bath for 30min. Weigh 120g of the water-bathed solution and add it dropwise to 45.3g of glacial acetic acid. During the dropwise addition, stir continuously on a magnetic stirrer. The prepared soybean protein solution is milky white without precipitation.

[0033] (2) Preparation of chitosan solution: take 20g of chitosan powder and dissolve it in 980g of 2% glacial acetic acid, stir evenly, leave it overnight until completely dissolved, and prepare 2% shell by centrifugal degassing at 7500rpm / min for 10min at 4°C Polysaccharide solution, set aside.

[0034] (3) Preparation of soybean protein / chitosan composite solution: Weigh 144.3g of the soybean protein solutio...

Embodiment 2

[0038] Example 2 In vitro degradability test of guiding periodontal tissue regeneration barrier membrane

[0039] (1) Prepare a phosphate buffer solution containing lysozyme (the content of lysozyme is 1.5 mg / mL), filter and sterilize and store at 4°C for later use.

[0040] (2) Weigh 0.1 g of the composite barrier membrane carrying the bioactive factor described in Example 1, place it in a centrifuge tube, and sterilize it by high-pressure steam;

[0041] (3) Under aseptic operation, add 10 mL of phosphate buffer solution containing lysozyme to the above centrifuge tube (2), place it on a constant temperature shaker at 37°C and shake at a low speed, and change half of the solution every day to ensure that the pH of the solution is relatively stable;

[0042] (4) Sampling and testing at 3 days, 1 week, 2 weeks, 3 weeks, 4 weeks, and 8 weeks respectively (set 3 parallel samples at each time point), gently rinse the taken out samples with double distilled water 3 times, Put in ...

Embodiment 3

[0046] Example 3 In vitro Promoting PDLSCs Proliferation and Differentiation Test of Guided Periodontal Tissue Regeneration Barrier Membrane

[0047] (1) Cut the composite barrier membrane carrying biologically active factors described in Example 1 into pieces, sterilize at 121° C. for 30 minutes by high-temperature and high-pressure steam, weigh 0.1 g of the composite barrier membrane and place it in 20 mL DMEM serum-free culture medium, and extract it for 2 days Take out the culture medium, centrifuge at 1000rpm / min for 10min, take the supernatant, which is the extract of the composite barrier membrane, and store it in a 4°C refrigerator for later use;

[0048] (2) Take the third-generation PDLSCs in a good growth state, digest them with trypsin, and prepare them at a density of 1×10 5 / mL cell suspension, inoculated into 96-well plate (200 μL per well) and 24-well plate (1 mL per well), respectively, at 37°C in 5% CO 2 Cultivate in the incubator for 24h;

[0049] (3) Afte...

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Abstract

The invention discloses a GPTR (Guided Periodontal Tissue Regeneration) barrier membrane, and a preparation method and application thereof, and belongs to the field of biomedical materials. The GPTR barrier membrane is a tissue regeneration barrier membrane which is of a double-layer asymmetric structure with a compact layer and a porous layer, and can be used for tissue repairing. Preparation of the GPTR barrier membrane comprises the following steps: carrying out tape casting on a soybean protein / chitosan complex solution, thus preparing the compact layer of a double-layer asymmetric barrier membrane; carrying out freeze drying on the compact layer, thus preparing the porous layer of a double-layer asymmetric composite barrier membrane; enabling the double-layer asymmetric composite barrier membrane to carry bioactive factors, thus obtaining the tissue regeneration barrier membrane. According to the GPTR barrier membrane and the preparation method, disclosed by the invention, the operation steps are simple, a preparation process of the tissue regeneration barrier membrane has no addition of toxic reagents of aldehydes and the like, a reaction system is moderate, and the bioactivity of two natural high polymer materials and the added bioactive factors can be better reserved.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a barrier film for guiding periodontal tissue regeneration and its preparation method and application. Background technique [0002] Periodontal disease is one of the two major diseases of the human oral cavity, and has a high prevalence in my country and the world. With the aging of our population, the problem of periodontal disease will become more prominent. The treatment of periodontal disease is not only to eliminate inflammation, but more importantly, to regenerate damaged periodontal tissue and form new periodontal attachments. How to achieve the complete regeneration of periodontal tissue is an unresolved problem, and it is also an important topic and breakthrough point in the field of periodontal disease research. Guided tissue regeneration (GTR) technology is one of the most commonly used and effective methods to solve this clinical problem. The use of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22A61L27/20A61L27/56A61L27/50A61L27/60A61L27/58
CPCA61L27/20A61L27/227A61L27/50A61L27/507A61L27/56A61L27/58A61L27/60A61L2430/02A61L2430/10A61L2430/32C08L89/00C08L5/08
Inventor 王小梅陈云李毅宋俊芳崔毅飞
Owner WUHAN INST OF BIOENG
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