Preparation method of chicken bursin
A technology of bursalin and chicken bursa, applied in peptide preparation methods, chemical instruments and methods, allergic diseases, etc., can solve the problems of failing to discover or understand the safety risks of chicken-origin viruses and large viruses
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Embodiment 1
[0041] A preparation method of chicken bursa, comprising the following steps:
[0042] 1) choose the bursa of healthy chicken, described chicken bursa has no avian leukemia virus, avian reticuloendothelial hyperplasia virus and chicken infectious bursal disease virus pollution, remove the fat, muscle of chicken bursa surface , fascia and other tissues, washed with water for injection, minced by a meat grinder, mixed with water for injection and minced chicken bursa, and homogenized by a colloid mill to obtain a homogenate solution.
[0043] 2) After the homogenate is disrupted by cells (repeated freeze-thaw disruption), centrifugation is performed to obtain a centrifugal supernatant, and a 0.22 μm filter membrane is used for tangential flow filtration to collect the permeate.
[0044] 3) Preparation of the crude product of chicken bursatin less than 5kDa: the permeate in step 2) is subjected to tangential flow filtration with a 5kDa filter membrane, and the permeate is collect...
Embodiment 2
[0050] A preparation method of chicken bursa, comprising the following steps:
[0051] 1) choose the bursa of healthy chicken, this chicken bursa has no avian leukemia virus, avian reticuloendothelial hyperplasia virus and chicken infectious bursal disease virus pollution, remove the fat, muscle, Fascia and other tissues are washed with water for injection, minced by a meat grinder, mixed with water for injection and minced chicken bursa, and homogenized by a colloid mill to obtain a homogenate.
[0052] 2) After the homogenate is crushed by a high pressure homogenizer, centrifugation is performed to obtain a centrifugal supernatant, and a 0.45 μm filter membrane is used for tangential flow filtration to collect the permeate.
[0053] 3) Preparation of less than 3kDa chicken bursin crude product: the permeate in step 2) is subjected to tangential flow filtration using a 50kDa filter, the permeate is collected and a 3KD filter is used to carry out tangential flow filtration, an...
Embodiment 3
[0058] A kind of preparation method of chicken bursa, it comprises the following steps:
[0059] 1) choose the bursa of healthy chicken, this chicken bursa has no avian leukemia virus, avian reticuloendothelial hyperplasia virus and chicken infectious bursal disease virus pollution, remove the fat, muscle, Fascia and other tissues are washed with water for injection, minced by a meat grinder, mixed with water for injection and minced chicken bursa, and homogenized by a colloid mill to obtain a homogenate.
[0060] 2) After the homogenate is disrupted by an ultrasonic cell disruptor, centrifugation is performed to obtain a centrifugal supernatant, and a 0.22 μm filter membrane is used for tangential flow filtration to collect the permeate.
[0061] 3) Preparation of the crude product of chicken bursatin less than 5kDa: the permeate in step 2) is subjected to tangential flow filtration with a 5kDa filter membrane, and the permeate is collected, which is the crude product of chic...
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