Preparation method of chicken bursin

A technology of bursalin and chicken bursa, applied in peptide preparation methods, chemical instruments and methods, allergic diseases, etc., can solve the problems of failing to discover or understand the safety risks of chicken-origin viruses and large viruses

Pending Publication Date: 2022-07-29
福建农业职业技术学院 +4
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing chicken BS manufacturing technology lacks a strict and systematic inspection of exogenous viruses on chicken bursae, resulting in a greater virus safety risk in the prepared chicken BS
[0008] (3) Due to the limitations of the technical level, there may still be chicken-derived viruses that have not been discovered or understood.
Existing chicken BS manufacturing technology lacks strict and systematic exogenous virus inspection for chicken BS, resulting in a greater virus safety risk in the prepared chicken BS

Method used

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  • Preparation method of chicken bursin
  • Preparation method of chicken bursin
  • Preparation method of chicken bursin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A preparation method of chicken bursa, comprising the following steps:

[0042] 1) choose the bursa of healthy chicken, described chicken bursa has no avian leukemia virus, avian reticuloendothelial hyperplasia virus and chicken infectious bursal disease virus pollution, remove the fat, muscle of chicken bursa surface , fascia and other tissues, washed with water for injection, minced by a meat grinder, mixed with water for injection and minced chicken bursa, and homogenized by a colloid mill to obtain a homogenate solution.

[0043] 2) After the homogenate is disrupted by cells (repeated freeze-thaw disruption), centrifugation is performed to obtain a centrifugal supernatant, and a 0.22 μm filter membrane is used for tangential flow filtration to collect the permeate.

[0044] 3) Preparation of the crude product of chicken bursatin less than 5kDa: the permeate in step 2) is subjected to tangential flow filtration with a 5kDa filter membrane, and the permeate is collect...

Embodiment 2

[0050] A preparation method of chicken bursa, comprising the following steps:

[0051] 1) choose the bursa of healthy chicken, this chicken bursa has no avian leukemia virus, avian reticuloendothelial hyperplasia virus and chicken infectious bursal disease virus pollution, remove the fat, muscle, Fascia and other tissues are washed with water for injection, minced by a meat grinder, mixed with water for injection and minced chicken bursa, and homogenized by a colloid mill to obtain a homogenate.

[0052] 2) After the homogenate is crushed by a high pressure homogenizer, centrifugation is performed to obtain a centrifugal supernatant, and a 0.45 μm filter membrane is used for tangential flow filtration to collect the permeate.

[0053] 3) Preparation of less than 3kDa chicken bursin crude product: the permeate in step 2) is subjected to tangential flow filtration using a 50kDa filter, the permeate is collected and a 3KD filter is used to carry out tangential flow filtration, an...

Embodiment 3

[0058] A kind of preparation method of chicken bursa, it comprises the following steps:

[0059] 1) choose the bursa of healthy chicken, this chicken bursa has no avian leukemia virus, avian reticuloendothelial hyperplasia virus and chicken infectious bursal disease virus pollution, remove the fat, muscle, Fascia and other tissues are washed with water for injection, minced by a meat grinder, mixed with water for injection and minced chicken bursa, and homogenized by a colloid mill to obtain a homogenate.

[0060] 2) After the homogenate is disrupted by an ultrasonic cell disruptor, centrifugation is performed to obtain a centrifugal supernatant, and a 0.22 μm filter membrane is used for tangential flow filtration to collect the permeate.

[0061] 3) Preparation of the crude product of chicken bursatin less than 5kDa: the permeate in step 2) is subjected to tangential flow filtration with a 5kDa filter membrane, and the permeate is collected, which is the crude product of chic...

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Abstract

The invention discloses a preparation method of chicken bursin, which comprises the following steps: 1) mixing water for injection with minced chicken bursa of Fabricius, and homogenizing by a colloid mill to obtain homogenate; 2) performing cell disruption on the homogenate, centrifuging, filtering, and collecting permeate; 3) carrying out tangential flow filtration on the permeate step by step by using filter membranes with different molecular weights of cut-off apertures, and collecting the permeate or cut-off to obtain required chicken bursin crude products with different molecular weight ranges; 4) mixing the chicken bursin crude products in at least one molecular weight range in the step 3) to obtain a chicken bursin primary product; and 5) removing viruses from the chicken bursin primary product, adjusting the pH value and osmotic pressure, filtering and sterilizing to obtain the chicken bursin. According to the method, step-by-step tangential flow filtration is adopted, an accurate and effective chicken bursin separation and concentration technology and a small molecule purification technology are established, and various chicken bursin with different molecular weights can be prepared as required by selecting filter membranes with different cut-off pore diameters and molecular weights according to different technological processes.

Description

technical field [0001] The invention belongs to the field of biological products, and in particular relates to a preparation method of chicken bursae. Background technique [0002] The Bursa of Fabricus (BF) was first discovered by the Italian anatomist Hieronymus Fabricius in 1621, hence the name. Because it shows the characteristics of gradual degeneration with sexual maturity, it was considered to be an organ related to reproductive function without attracting attention for a long time. In 1956, Glick et al. found that the removal of BF would reduce the generation of antibodies against Salmonella O antigen in chicks, and the bursa was recognized as a unique central immune organ in birds. Subsequently, many scholars conducted in-depth research on BF in the aspects of morphological development, immunology, biological activity of tissue extracts, etc., and confirmed that BF is the place for the development, differentiation and maturation of B lymphocytes. It migrates from ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C07K1/34C07K1/36C12N7/06C12N7/04A61K35/57A61P37/04
CPCC07K1/145C07K1/34C07K1/36C12N7/00A61K35/57A61P37/04C12N2740/11061C12N2740/11063C12N2720/00061C12N2720/00063C12N2740/13061C12N2740/13063
Inventor 徐磊林映升钟佳莲刘道泉廖惠珍余勋信陈上永刘毅发谢杼倢傅秋玲钟云钦王宗冬傅光华黄瑜张渊魁
Owner 福建农业职业技术学院
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