85 results about "Reproductive function" patented technology

The invention provides application of mitochondrial acid 5 in preparation of a medicine for treating reproductive organ injury caused by diabetes mellitus, and relates to the technical field of biological medicine. Experiments prove that as an artificially synthesized indole-3-acetic acid derivative, MA-5 provides a brand new treatment thought for testis injury caused by diabetes mellitus, not only is beneficial to improvement of reproductive function, but also possibly provides a new strategy for overall treatment of diabetes mellitus. Besides, as a natural plant hormone, MA-5 has relatively high safety and acceptability, and possibly becomes a new alternative therapy with a clinical application prospect. Although the long-term administration of the MA-5 does not significantly influence the blood glucose level, the alleviation effect of the MA-5 on testis injury shows that the MA-5 as a treatment means for long-term use can continuously improve reproductive dysfunction caused by diabetes, and has potential clinical advantages.

The invention relates to the technical field of feed additives, and discloses a feed combination agent for improving the reproductive performance of sows by using 25-OH-VD3 and a preparation method thereof, and the preparation method comprises the following steps: taking an innovative product 25-OH-VD3 as a main raw material, and optimally combining other micronutrients, immune substances, metabolism promoting substances and the like for cooperative utilization to prepare the combination agent. The innovative 25-OH-VD3 not only serves as a golden partner for calcium and phosphorus metabolism of an organism, but also plays a wide immune regulation function, improves the reproductive function of pigs together and strengthens an optimized feed combination method and composition of the main effect of the 25-OH-VD3 at the same time, and other vitamins, trace elements, targeted active substances and other optimized combinations are preferably selected. The feed mainly solves the technical problem of integration of health and reproductive disorder of modern pig raising, and has the effects of remarkably enhancing the immunity of the sows, resisting oxidation and stress and systematically improving the reproductive performance of the sows.

The invention discloses a preparation method and application of oyster peptide for improving human fertility quality. Fresh oysters are used as raw materials, protease hydrolysis is utilized to release small molecule peptides, and then separation, purification and activity research are performed to explore the good utilization value of the oysters in the aspect of improving the reproductive function; the invention aims at innovating an oyster peptide extraction method to obtain the oyster peptide with high biological activity and high absorptivity, and discloses and introduces the application of the oyster peptide in the aspect of high human fertility quality. Meanwhile, the oyster peptide has the effects of improving the reproductive function, supplementing nutrition, mineral substances, protein, vitamins and the like, can be added into food, health care products, medicines and the like, and is a multifunctional biological peptide.

The invention belongs to the technical field of biological medicine and livestock breeding, and particularly relates to application of anthocyanin in improving the reproductive function of female beef cattle by adjusting intestinal flora and reducing follicular granule cell apoptosis. Specifically, the invention discovers and verifies for the first time through research that the anthocyanin added into the feed can play roles in regulating the reproductive hormone level of the cattle with the ovarian dysfunction, improving the ovarian function and improving the intestinal flora lineage, and meanwhile, the anthocyanin can resist the apoptosis of ovarian granular cells by resisting oxidative stress injury, so that the reproductive function of the cattle is improved. The invention provides a scientific basis for the application of the anthocyanin in the aspect of improving the reproductive capacity of the female beef cattle, and discloses a mechanism for improving the ovarian function by resisting oxidation and regulating intestinal flora, thereby being beneficial to improving the application prospect of the anthocyanin in animal husbandry, and having a good practical application value.

The invention provides a functional peptide with a yang tonifying effect, a seal penis peptide and a preparation method and application of the functional peptide and the seal penis peptide. The functional peptide comprises a peptide fragment pER and can promote testicular interstitial cells to secrete testosterone. Testosterone is steroid hormone and can be secreted by testicular interstitial cells through enzymatic reaction. The low testosterone level in serum can cause male testicular atrophy and low sperm survival rate, so that the male reproductive function is influenced, and the phenomena of poor sexual desire, premature ejaculation, erectile dysfunction and the like can also be caused. In addition, the testosterone also has the effects of maintaining muscle strength and quality, maintaining bone density and strength, refreshing, improving physical ability and the like. Therefore, the functional peptide provided by the invention has the effect of promoting the secretion of testosterone, which means that the functional peptide not only can effectively improve the male sexual function, but also can alleviate the damage of male sexual dysfunction to the body and spirit.

The invention discloses application of rapamycin combined with a vascular endothelial growth factor A in improvement of an ovarian tissue function. The improvement of the ovarian tissue function comprises any one or more than two of the following aspects: increasing the survival rate of follicles, prolonging the functional life of the ovarian tissue and improving the quality of oocytes. The effective concentration of the rapamycin is 500nM, and the effective concentration of the vascular endothelial growth factor A is 5ng / mL. The follicle loss dynamic after OTCT is tracked through a mouse model, it is found that rapid follicle loss is related to tip cell damage and primordial follicle activation, then a rapamycin and vascular endothelial growth factor A combination strategy is provided, follicle loss can be remarkably reduced, the reproductive function period can be prolonged, and the oocyte quality can be improved; according to the invention, a systematic solution is provided for OTCT technology optimization.

The invention relates to the technical field of medicine application, and discloses a medicine composition for preventing or treating reproductive dysfunction of elderly women and application of the medicine composition. The invention provides a new therapeutic drug and means for prevention and treatment of reproductive dysfunction of elderly women. By constructing an animal model of reproductive dysfunction of the elderly women and taking the ovulation number of animals, hormone level related to reproduction, pregnancy rate and progeny development condition as detection indexes, the pharmacodynamic effect and safety of the pharmaceutical composition and the influence of the pharmaceutical composition on progeny development quality and genetic variability are evaluated for the first time; the effectiveness and safety of the pharmaceutical composition for treating reproductive dysfunction of elderly women are fully proved.

The invention discloses a construction method of a zebra fish reproductive toxicity model, a method for evaluating reproductive toxicity of chemical substances and application, and relates to the technical field of reproductive development toxicity of chemical substances. According to the method for evaluating the reproductive toxicity of the chemical substance provided by the invention, the change of a diatrix behavior can be concerned by shooting behavioral pictures or videos without sacrifice of an experimental animal or professional fluorescence imaging instrument equipment and tissue slicing equipment; the method is more in line with animal ethics, cost is saved, and continuous observation can be realized by using the same batch of organisms. The test courtship behavior can quickly utilize software to analyze and obtain zebra fish behavior changes. By adopting the evaluation method provided by the invention, both female ovarian malformation and male reproductive toxicity can be detected, and the evaluation method has the advantage of more evaluation dimensions. Based on the method provided by the invention, a zebrafish reproductive toxicity model can be constructed, and the model can be used as a drug screening platform for reproductive function damage of zebrafish.

This invention relates to the field of beauty and skincare, and discloses an automated system and method for detecting semen quality, comprising: a morphological defect rate calculation module for calculating the morphological defect rate corresponding to a target semen sample; a dysfunctional sperm identification module for identifying dysfunctional sperm in the target semen sample; a reproductive function scoring module for evaluating the reproductive function score corresponding to the target semen sample; a dynamic motility potential scoring module for configuring a suitable culture medium for the target semen sample, culturing the target semen sample in the suitable culture medium, recording the sperm viability baseline parameters during the culture process, calculating the sperm survival rate of the target semen sample, and evaluating the dynamic motility potential score corresponding to the target semen sample; and a semen quality analysis module for generating a semen quality analysis report corresponding to the target semen sample. This invention can improve the accuracy of semen quality detection.

The present application relates to the technical field of oyster peptides, and specifically to a method for preparing oyster peptides and their use in improving male reproductive function. The present application utilizes oyster peptides as raw materials, grafts oleanolic acid saponins thereon, and composites them with chitosan to prepare an emulsion of a ternary complex. The emulsion can form stable microscopic particles and has stable rheological properties. Through simulated digestion experiments, it was found that the emulsion has a sustained-release effect on the digestion of oyster peptides, and can at least reduce the proportion of digestion in the oral cavity. In addition, the use of the emulsion and the oyster peptide to intervene in rats with nephrotic syndrome can effectively alleviate their nephrotic syndrome, and at the same time promote the male sexual function of rats, and has an application prospect of modifying and improving male reproductive function.

The invention provides a traditional Chinese medicine composition for polycystic ovarian syndrome lipid metabolism disorder and application. The traditional Chinese medicine composition is prepared from the following raw material medicines in parts by weight: 125 to 500 parts of rhizoma atractylodis macrocephalae, 75 to 300 parts of radix bupleuri, 150 to 600 parts of herba artemisiae scopariae, 75 to 300 parts of fructus gardeniae, 125 to 500 parts of fructus citri sarcodactylis, 50 to 200 parts of fried semen ziziphi spinosae, 50 to 200 parts of fructus schizandrae, 75 to 300 parts of herba sedi and 75 to 300 parts of raw liquorice root. The traditional Chinese medicine composition disclosed by the invention is reasonable in proportion, can improve the reproductive function and lipid metabolism disorder of a mouse model with polycystic ovarian syndrome, and can improve the levels of triglyceride, cholesterol, free fatty acid and the like in HepG2 cell supernatant induced by DHT (docosahexaenoic acid). The traditional Chinese medicine composition disclosed by the invention is supported by a solid traditional Chinese medicine gynecologic classic theory and clinical and laboratory data, is definite in clinical curative effect, free of toxic and side effects and safe to take, and can be applied to preparation of medicines for treating polycystic ovarian syndrome lipid metabolism disorder.

The invention relates to an effect of an REV-ERBalpha agonist SR9009 in the aspect of delaying ovarian senescence, and aims at the problem of fertility decline caused by cliff-type decline of ovarian functions of women over 35 years old, the SR9009 is applied to activate an REV-ERBalpha circadian rhythm signal channel in ovarian tissues, inhibit excessive autophagy of granular cells and maintain a primordial follicle dormancy state, so that the agonist SR9009 can be used for delaying ovarian senescence. Therefore, the original follicle depletion speed is delayed, follicle storage is maintained, and the ovary reproductive function cycle is prolonged. The effect is verified by a mammal model: REV-ERBalpha protein expression in an ovary of an aging individual is reduced, rhythmicity expression is disordered, autophagy of granulosa cells can be effectively inhibited in the ovary of the aging individual intervened by the SR9009, and the number of residual primordial follicles and the number of total follicles are obviously greater than those of an untreated control group. The invention provides an innovative treatment strategy of targeted rhythm regulation and control for clinical intervention of ovarian aging.

The invention belongs to the technical field of biological medicines, and particularly discloses application of roxburgh rose glycoside in preparation of a medicine for improving dyszoospermia. Pharmacodynamic verification is carried out by constructing various animal models with dyszoospermia, and it is found that the roxburgh rose glycoside can significantly improve reproductive function indexes, for example, the roxburgh rose glycoside can significantly increase the density of testicular interstitial cells, the thickness of spermatogenic epithelium and the density of sperms in epididymis lumens. As a main active component of a medicinal and edible plant roxburgh rose, the roxburgh rose glycoside has good biological safety and human body tolerance. The new application of the roxburgh rose glycoside in the field of reproductive medicine is disclosed for the first time, a novel candidate medicine is provided for treating dyszoospermia, and meanwhile, an application system of the roxburgh rose glycoside in functional health care products and healthy food additives is constructed.

The invention belongs to the technical field of medicines, and particularly relates to a pharmaceutical composition for improving ovarian function damage and degeneration and a preparation method thereof. The invention discloses the GnRH agonist effect of dihydromyricetin DMY for the first time, and the affinity of dihydromyricetin DMY to a GnRH receptor and the GnRH agonist effect of dihydromyricetin DMY are verified through in-vitro experiments. Model animal experiments further prove that the dihydromyricetin DMY has a protective effect on ovarian function impairment caused by chemotherapy. Experiments prove that the dihydromyricetin DMY is given while the cyclophosphamide (CTX) is used for chemotherapy, so that the damage of the dihydromyricetin DMY to the ovarian function during chemotherapy can be reduced, and a protection effect is also achieved on the recovery of the ovarian function after drug withdrawal. The protection effect is reflected on the endocrine function and the reserve function of the ovary, and the preserved ovary reserve function can achieve the pregnancy result after chemotherapy is finished, which indicates that the dihydromyricetin DMY also plays the protection effect on the fertility function of the ovary.

The application discloses a kind of complex fermentation inoculant for improving equol production, fermentation liquor and application thereof, belong to microbial fermentation technical field.The complex fermentation inoculant of the application, streptococcus thermophilus BLCC2-0025 and lactococcus lactis MP1001 have high β-glucosidase activity, and low activity glycoside form is converted into high activity daidzein;Salivary lactobacillus LS1101 and lactobacillus gregius 0010 have the activity of further converting daidzein into equol;Four strains of bacteria are used in combination, which can synergistically improve the content of equol.The fermentation liquor prepared by fermenting pueraria extract with the complex fermentation inoculant of the application can target regulate intestine-ovary axis, significantly improve the reproductive function of POF mice, relieve ovarian oxidative stress and accurately regulate intestinal flora balance, providing a safe and effective preparation and application scheme for non-hormone intervention of premature ovarian failure.

The invention discloses application of a caenorhabditis elegans reproductive disorder model to screening of strains promoting reproduction health, and relates to the technical field of biologica.Cyclophosphamide medicine intervention is applied to caenorhabditis elegans, so that the normal reproductive system of caenorhabditis elegans is damaged, and the reproductive disorder model is prepared; then 3985 gene knockout strains in an escherichia coli Keio gene knockout library are utilized, and strains with the reproductive capacity remarkably improved for reproductive disorder are screened; and carrying out homologous gene knockout on the probiotics by using a gene editing technology, and screening out the probiotics with the potential of promoting reproductive health. The reproductive disorder model is stable and repeatable, can be used for screening strains or drugs for improving the reproductive function, and also can be used for screening candidate strains or drugs for reducing the reproductive toxicity of cyclophosphamide. According to the method, an effective tool is provided for screening of reproduction-related probiotic strains or screening of related drugs, and meanwhile, the strains screened based on the model provide new candidate strains for treatment and research of reproductive disorder diseases.

The invention provides medicinal and edible liqueur capable of improving reproductive function, tonifying Yang and kidney, tonifying Qi and replenishing deficiency, and relates to the field of blended wine. The fen-flavor rice wine comprises the following raw materials in parts by weight: base wine: 3.5-5 parts of yellow wine, 1-3 parts of fen-flavor rice liquor and 3-5 parts of water; the traditional Chinese medicinal material powder comprises 0.5 to 1.5 parts of honey, 0.2 to 1.0 part of ginseng powder, 0.2 to 1.0 part of American ginseng powder, 0.2 to 1.0 part of cistanche powder, 0.2 to 1.0 part of Chinese yam powder, 0.2 to 1.0 part of wolfberry powder, 0.2 to 1.0 part of deer blood powder, 0.1 to 0.5 part of rhizoma polygonati powder, 0.1 to 0.5 part of cordyceps militaris powder, 0.1 to 0.5 part of clove powder, 0.1 to 0.5 part of cinnamon powder, 0.1 to 0.5 part of raspberry powder, 0.1 to 0.5 part of sweet-scented osmanthus powder, 0.1 to 0.5 part of eucommia ulmoides leaf powder and 0.1 to 0.5 part of orange peel powder. The treated traditional Chinese medicinal material powder is added into the base liquor, so that pesticide residues, bitter taste and the like in the medicinal materials can be removed, and the taste of the liqueur is richer.

The invention discloses a composition for improving the production performance of hens and application thereof, and the composition for improving the production performance of hens comprises the following components in parts by mass: 5-9 parts of a motherwort-caulis spatholobi composite extract and 1-3 parts of a quamoclit vine extract. The herba leonuri, the caulis spatholobi and the quamoclit climoclit are compounded for the first time, the herba leonuri prefers on reproductive health conditioning, the caulis spatholobi prefers on blood replenishing and oxidation resistance, the quamoclit climoclit prefers on antibacterial and anti-inflammatory effects, the advantages of the herba leonuri, the caulis spatholobi and the quamoclit climoclit are superposed, the reproductive function and overall health of hens are improved in a targeted mode, and the defect of a single traditional Chinese medicine on the broad-spectrum function is overcome.

Provided is the use of ginsenoside in the preparation of a composition for treating or preventing neurodegeneration. Also provided is the use of ginsenoside in the preparation of a composition for improving ovarian reproductive function, or improving mechanical and / or olfactory sensitivity in a subject. The ginsenoside is Rg3 or Rg5.

The invention relates to a swimming bladder hydrolysate capable of improving a male reproductive function as well as a preparation method and application thereof, and belongs to the technical field of biology. The invention provides a swimming bladder hydrolysate capable of improving a male reproductive function. The swimming bladder hydrolysate is obtained by performing enzymolysis on sturgeon swimming bladders through trypsin. The total protein content of the swimming bladder hydrolysate is 80%-90%; the amino acid composition of the swimming bladder hydrolysate comprises glycine, proline, glutamic acid, asparaginic acid, arginine, alanine, serine, lysine, threonine, phenylalanine, leucine, isoleucine, valine, histidine, tyrosine and methionine. Researches show that the swim bladder hydrolysate has very strong antioxidant activity, and the swim bladder hydrolysate can effectively relieve matt hair, unstable spirit, reduced exploration behavior, weight loss and male reproductive function damage of model mice caused by exposure of heavy metal cadmium, and has great application prospects.

This invention discloses a compound moxa stick for warming yang and unblocking obstruction, its preparation method, and its application in improving reproductive system function. It belongs to the field of external preparations of traditional Chinese medicine. Addressing the problems of limited improvement effects of pure moxa sticks, lack of systematic design in compound moxa stick formulations, unclear synergistic effects between components, large fluctuations in conditioning effects, and difficulty in balancing conditioning effects and safety, this invention uses specific weight proportions of Artemisia argyi, Cyperus rotundus, Alpinia galanga, Cinnamomum cassia, Zingiber officinale, Illicium verum, Cinnamomum cassia twig, Spatholobus suberectus, Caesalpinia sappan, Smilax glabra, Euonymus fortunei, Citrus aurantium, Amomum villosum, Citrus reticulata peel, and Atractylodes lancea to prepare a compound moxa stick. The components synergistically enhance each other, and this product is used to prepare a moxibustion intervention product for improving the reproductive function of mammals. It can stably improve female ovarian reserve function and male asthenospermia, simultaneously regulate reproductive-related hormone levels, repair pathological damage to reproductive organs, and improve the quality of reproductive cells.

The invention provides a construction method of a viral reproductive dysfunction model based on spermatogonial stem cells and application of the model, and belongs to the cross technical field of biotechnology and veterinary medicine. The construction method comprises the following steps: placing spermatogonial stem cells in a spermatogonial stem cell culture medium for culture, and infecting the spermatogonial stem cells with bovine viral diarrhea viruses to obtain the viral reproductive function disorder model based on the spermatogonial stem cells. In order to solve the problems that in the prior art, a BVDV reproductive injury mechanism is unknown, and an effective research model is lacked, the reproductive stem cells are used for simulating direct injury of BVDV to a male reproductive system for the first time, and the defect that a traditional model (such as MDBK cells) cannot reflect specific biological reaction of reproductive cells is overcome; meanwhile, experiments show that the model constructed by the invention can support standardized processes of virus strain typing and drug screening, the research and development efficiency is remarkably improved, and the experiment cost is reduced.

This application provides the use of purine nucleoside phosphorylase inhibitors in improving the reproductive function of subjects. This application provides the use of purine nucleoside phosphorylase inhibitors in the preparation of drugs for antagonizing hydrosalpinx toxicity or improving the reproductive function of subjects. This application also provides the use of purine nucleoside phosphorylase inhibitors in the preparation of reagents for embryo culture and gamete treatment. This application utilizes purine nucleoside phosphorylase inhibitors to inhibit PNP activity, antagonizing hydrosalpinx toxicity caused by purine metabolism disorders, thereby improving the reproductive function of subjects.

The invention discloses a novel application of a compound beta-alanine (beta-alanine) in preparation of a medicine for improving oocyte quality and treating ovarian endometriosis (OEM) related infertility, and belongs to the technical field of biological medicines. An OEM animal model proves that beta-alanine can remarkably improve reproductive function disorder of model animals, specifically, follicular dysplasia caused by endometriosis is effectively relieved, the fertility is improved, and the litter size is increased; in the aspect of oocyte quality, the compound can promote normal discharge of a first polar body in an oocyte in-vitro maturation process, reduce cytoplasm fragmentation, recover spindle body form and microtubule arrangement, enhance mitochondrial membrane potential stability and maintain the integrity of an ultrastructure. The results show that beta-alanine can reverse oocyte quality reduction caused by ovarian endometriosis in multiple dimensions, has a definite treatment potential, and provides an important candidate molecule for developing a novel assisted reproduction intervention strategy for targeting oocyte health.

This invention provides a traditional Chinese medicine composition for anti-aging and improving reproductive function, comprising 11 raw materials: Astragalus membranaceus, Atractylodes macrocephala, Leonurus japonicus, Platycladus orientalis seed, Angelica sinensis, Lycium barbarum, Polygonatum sibiricum, Cuscuta chinensis, Astragalus complanatus, Morinda officinalis, and Panax quinquefolius, in a specific weight ratio. This invention divides the above-mentioned raw materials into four drug units: tonifying the kidney and replenishing essence, invigorating qi and nourishing blood, strengthening the spleen and promoting digestion, and nourishing the heart and calming the mind. These units form a positive reinforcing cycle and together constitute a synergistic network, achieving a synergistic effect of delaying overall aging and improving reproductive function.

The invention relates to the technical field of oyster peptides, in particular to a preparation method of oyster peptides and application of the oyster peptides in improvement of male reproductive functions. Oyster peptide is used as a raw material, oleanolic acid type saponin grafting is carried out on the oyster peptide, and the oyster peptide is compounded with chitosan to prepare the ternary complex emulsion. The emulsion can form stable microscopic particles and has stable rheological properties. Simulated digestion experiments show that the emulsion has a slow release effect on digestion of oyster peptide, and at least can reduce the digestion proportion of the oyster peptide in the oral cavity. Besides, when the emulsion and the oyster peptide are used for intervening the rats with the nephrotic syndrome, the nephrotic syndrome can be effectively relieved, meanwhile, the effect of promoting the male sexual function of the rats is achieved, and the oyster peptide has the application prospect of modifying and improving the male reproductive function.

The present application relates to the technical field of reproductive function state evaluation, and particularly relates to a reproductive function state evaluation system based on multi-modal physiological data. The system comprises: a data acquisition module for acquiring blood glucose, heart rate, acceleration data and follicle diameter; a data analysis module for dividing sleep and wake-up periods, calculating blood glucose deficiency degree in the sleep period, and determining the correlation of blood glucose and heart rate reverse linkage events in the wake-up period to determine the synchronization degree of nerve-metabolic regulation stiffness; a physiological resistance index acquisition module for calculating the physiological resistance index according to the blood glucose deficiency degree, the reverse linkage frequency gained by the synchronization degree, and the follicle diameter susceptibility weight; and a data processing module for obtaining the evaluation result in combination with the follicle growth condition and the physiological resistance index. The present application quantifies the nerve-metabolic stiffness and the environmental resistance, solves the problem that it is difficult to distinguish between insufficient drug dosage and excessive body environmental resistance, and effectively improves the accuracy of reproductive function state evaluation.

The invention discloses a pregnancy-assisting health-care medicine and food homology formula. The invention belongs to the technical field of health care. The formula comprises the following components in parts by weight: 5-60g of wine-processed radix angelicae sinensis, 5-60g of fried radix paeoniae rubra, 5-60g of roasted cassia twig, 5-60g of processed rhizoma cyperi, 5-60g of baked ginger charcoal, 5-60g of fried polygala tenuifolia, 5-60g of roasted radix aucklandiae, 5-60g of salted fennel, 5-60g of fried eucommia ulmoides, 5-60g of deep-fried rhizoma atractylodis macrocephalae, 5-60g of fried radix dipsaci, 5-60g of salted semen cuscutae, 5-60g of processed herba epimedii, 5-60g of fried radix achyranthis bidentatae, 5-60g of herba lycopi and 5-60g of fructus corni. 5-60 g of motherwort herb; the traditional Chinese medicine adopts medicinal and edible raw materials, is safe and reliable, does not cause obvious toxic and side effects on the body after long-term taking, and is suitable for long-term body conditioning of pregnancy preparation people; the synergistic effect of multiple raw materials plays a role in regulating endocrine, improving the blood circulation of a reproductive system, enhancing the reproductive function and the like, and the pregnancy probability is effectively increased; appropriate adjustment can be carried out according to personal physique and symptoms, and high adaptability and flexibility are achieved.

The invention provides application of trehalose in preparation of a medicine for improving reproductive organ injury caused by diabetes mellitus, and relates to the technical field of biological medicine. As an innovative autophagy activator, trehalose provides a new treatment thought for testis injury caused by diabetes mellitus, has the advantages of small side effect, high safety and the like, and is expected to become an effective replacement therapy in clinical application. Although the long-term administration of the trehalose does not significantly influence the blood glucose level, the relieving effect of the trehalose on testis injury shows that the trehalose can continuously improve reproductive dysfunction caused by diabetes mellitus as a treatment means for long-term use, and has potential clinical advantages.

The invention relates to the technical field of peptide extraction, in particular to sipunculus nudus peptide and an extraction method and application thereof. According to the specific technical scheme, the method comprises the following steps: 1) crushing a sipunculus nudus raw material, adding water, adding sodium hydrogen sulfite and ascorbic acid, and stirring and soaking for 10-15 minutes; 2) grinding the soaked raw materials, and adjusting the pH value of the slurry to be alkaline; (3) adding alkali enzyme into the slurry, carrying out microwave enzymolysis for 45-50 minutes, adding papaya peptidase B, continuously carrying out microwave enzymolysis for 45-50 minutes, heating, inactivating, cooling, carrying out centrifugal filtration, and collecting filtrate; and 4) sequentially carrying out microfiltration, ultrafiltration and sodium filtration on the obtained filtrate to obtain the product. Microwave radiation is adopted for assisting enzymolysis, energy can be provided for an enzymolysis system, the temperature needed by enzymolysis can be maintained, the enzymolysis efficiency is greatly improved, the prepared sipunculus nudus peptide can stabilize iron ions, a safe and efficient iron supplementing preparation can be developed, meanwhile, reproductive function decline caused by obesity can be comprehensively repaired, the advantage of multi-dimensional conditioning is achieved, and the sipunculus nudus peptide has a good application prospect. And the method is suitable for metabolism-related reproductive disorder treatment.