46 results about "Reproductive function" patented technology

This invention relates to the field of beauty and skincare, and discloses an automated system and method for detecting semen quality, comprising: a morphological defect rate calculation module for calculating the morphological defect rate corresponding to a target semen sample; a dysfunctional sperm identification module for identifying dysfunctional sperm in the target semen sample; a reproductive function scoring module for evaluating the reproductive function score corresponding to the target semen sample; a dynamic motility potential scoring module for configuring a suitable culture medium for the target semen sample, culturing the target semen sample in the suitable culture medium, recording the sperm viability baseline parameters during the culture process, calculating the sperm survival rate of the target semen sample, and evaluating the dynamic motility potential score corresponding to the target semen sample; and a semen quality analysis module for generating a semen quality analysis report corresponding to the target semen sample. This invention can improve the accuracy of semen quality detection.

The invention relates to an effect of an REV-ERBalpha agonist SR9009 in the aspect of delaying ovarian senescence, and aims at the problem of fertility decline caused by cliff-type decline of ovarian functions of women over 35 years old, the SR9009 is applied to activate an REV-ERBalpha circadian rhythm signal channel in ovarian tissues, inhibit excessive autophagy of granular cells and maintain a primordial follicle dormancy state, so that the agonist SR9009 can be used for delaying ovarian senescence. Therefore, the original follicle depletion speed is delayed, follicle storage is maintained, and the ovary reproductive function cycle is prolonged. The effect is verified by a mammal model: REV-ERBalpha protein expression in an ovary of an aging individual is reduced, rhythmicity expression is disordered, autophagy of granulosa cells can be effectively inhibited in the ovary of the aging individual intervened by the SR9009, and the number of residual primordial follicles and the number of total follicles are obviously greater than those of an untreated control group. The invention provides an innovative treatment strategy of targeted rhythm regulation and control for clinical intervention of ovarian aging.

The invention belongs to the technical field of medicines, and particularly relates to a pharmaceutical composition for improving ovarian function damage and degeneration and a preparation method thereof. The invention discloses the GnRH agonist effect of dihydromyricetin DMY for the first time, and the affinity of dihydromyricetin DMY to a GnRH receptor and the GnRH agonist effect of dihydromyricetin DMY are verified through in-vitro experiments. Model animal experiments further prove that the dihydromyricetin DMY has a protective effect on ovarian function impairment caused by chemotherapy. Experiments prove that the dihydromyricetin DMY is given while the cyclophosphamide (CTX) is used for chemotherapy, so that the damage of the dihydromyricetin DMY to the ovarian function during chemotherapy can be reduced, and a protection effect is also achieved on the recovery of the ovarian function after drug withdrawal. The protection effect is reflected on the endocrine function and the reserve function of the ovary, and the preserved ovary reserve function can achieve the pregnancy result after chemotherapy is finished, which indicates that the dihydromyricetin DMY also plays the protection effect on the fertility function of the ovary.

The application discloses a kind of complex fermentation inoculant for improving equol production, fermentation liquor and application thereof, belong to microbial fermentation technical field.The complex fermentation inoculant of the application, streptococcus thermophilus BLCC2-0025 and lactococcus lactis MP1001 have high β-glucosidase activity, and low activity glycoside form is converted into high activity daidzein;Salivary lactobacillus LS1101 and lactobacillus gregius 0010 have the activity of further converting daidzein into equol;Four strains of bacteria are used in combination, which can synergistically improve the content of equol.The fermentation liquor prepared by fermenting pueraria extract with the complex fermentation inoculant of the application can target regulate intestine-ovary axis, significantly improve the reproductive function of POF mice, relieve ovarian oxidative stress and accurately regulate intestinal flora balance, providing a safe and effective preparation and application scheme for non-hormone intervention of premature ovarian failure.

The invention discloses application of a caenorhabditis elegans reproductive disorder model to screening of strains promoting reproduction health, and relates to the technical field of biologica.Cyclophosphamide medicine intervention is applied to caenorhabditis elegans, so that the normal reproductive system of caenorhabditis elegans is damaged, and the reproductive disorder model is prepared; then 3985 gene knockout strains in an escherichia coli Keio gene knockout library are utilized, and strains with the reproductive capacity remarkably improved for reproductive disorder are screened; and carrying out homologous gene knockout on the probiotics by using a gene editing technology, and screening out the probiotics with the potential of promoting reproductive health. The reproductive disorder model is stable and repeatable, can be used for screening strains or drugs for improving the reproductive function, and also can be used for screening candidate strains or drugs for reducing the reproductive toxicity of cyclophosphamide. According to the method, an effective tool is provided for screening of reproduction-related probiotic strains or screening of related drugs, and meanwhile, the strains screened based on the model provide new candidate strains for treatment and research of reproductive disorder diseases.

Provided is the use of ginsenoside in the preparation of a composition for treating or preventing neurodegeneration. Also provided is the use of ginsenoside in the preparation of a composition for improving ovarian reproductive function, or improving mechanical and / or olfactory sensitivity in a subject. The ginsenoside is Rg3 or Rg5.

This invention discloses a compound moxa stick for warming yang and unblocking obstruction, its preparation method, and its application in improving reproductive system function. It belongs to the field of external preparations of traditional Chinese medicine. Addressing the problems of limited improvement effects of pure moxa sticks, lack of systematic design in compound moxa stick formulations, unclear synergistic effects between components, large fluctuations in conditioning effects, and difficulty in balancing conditioning effects and safety, this invention uses specific weight proportions of Artemisia argyi, Cyperus rotundus, Alpinia galanga, Cinnamomum cassia, Zingiber officinale, Illicium verum, Cinnamomum cassia twig, Spatholobus suberectus, Caesalpinia sappan, Smilax glabra, Euonymus fortunei, Citrus aurantium, Amomum villosum, Citrus reticulata peel, and Atractylodes lancea to prepare a compound moxa stick. The components synergistically enhance each other, and this product is used to prepare a moxibustion intervention product for improving the reproductive function of mammals. It can stably improve female ovarian reserve function and male asthenospermia, simultaneously regulate reproductive-related hormone levels, repair pathological damage to reproductive organs, and improve the quality of reproductive cells.

This application provides the use of purine nucleoside phosphorylase inhibitors in improving the reproductive function of subjects. This application provides the use of purine nucleoside phosphorylase inhibitors in the preparation of drugs for antagonizing hydrosalpinx toxicity or improving the reproductive function of subjects. This application also provides the use of purine nucleoside phosphorylase inhibitors in the preparation of reagents for embryo culture and gamete treatment. This application utilizes purine nucleoside phosphorylase inhibitors to inhibit PNP activity, antagonizing hydrosalpinx toxicity caused by purine metabolism disorders, thereby improving the reproductive function of subjects.

The invention discloses a novel application of a compound beta-alanine (beta-alanine) in preparation of a medicine for improving oocyte quality and treating ovarian endometriosis (OEM) related infertility, and belongs to the technical field of biological medicines. An OEM animal model proves that beta-alanine can remarkably improve reproductive function disorder of model animals, specifically, follicular dysplasia caused by endometriosis is effectively relieved, the fertility is improved, and the litter size is increased; in the aspect of oocyte quality, the compound can promote normal discharge of a first polar body in an oocyte in-vitro maturation process, reduce cytoplasm fragmentation, recover spindle body form and microtubule arrangement, enhance mitochondrial membrane potential stability and maintain the integrity of an ultrastructure. The results show that beta-alanine can reverse oocyte quality reduction caused by ovarian endometriosis in multiple dimensions, has a definite treatment potential, and provides an important candidate molecule for developing a novel assisted reproduction intervention strategy for targeting oocyte health.

The present application relates to the technical field of reproductive function state evaluation, and particularly relates to a reproductive function state evaluation system based on multi-modal physiological data. The system comprises: a data acquisition module for acquiring blood glucose, heart rate, acceleration data and follicle diameter; a data analysis module for dividing sleep and wake-up periods, calculating blood glucose deficiency degree in the sleep period, and determining the correlation of blood glucose and heart rate reverse linkage events in the wake-up period to determine the synchronization degree of nerve-metabolic regulation stiffness; a physiological resistance index acquisition module for calculating the physiological resistance index according to the blood glucose deficiency degree, the reverse linkage frequency gained by the synchronization degree, and the follicle diameter susceptibility weight; and a data processing module for obtaining the evaluation result in combination with the follicle growth condition and the physiological resistance index. The present application quantifies the nerve-metabolic stiffness and the environmental resistance, solves the problem that it is difficult to distinguish between insufficient drug dosage and excessive body environmental resistance, and effectively improves the accuracy of reproductive function state evaluation.

The invention relates to the technical field of peptide extraction, in particular to sipunculus nudus peptide and an extraction method and application thereof. According to the specific technical scheme, the method comprises the following steps: 1) crushing a sipunculus nudus raw material, adding water, adding sodium hydrogen sulfite and ascorbic acid, and stirring and soaking for 10-15 minutes; 2) grinding the soaked raw materials, and adjusting the pH value of the slurry to be alkaline; (3) adding alkali enzyme into the slurry, carrying out microwave enzymolysis for 45-50 minutes, adding papaya peptidase B, continuously carrying out microwave enzymolysis for 45-50 minutes, heating, inactivating, cooling, carrying out centrifugal filtration, and collecting filtrate; and 4) sequentially carrying out microfiltration, ultrafiltration and sodium filtration on the obtained filtrate to obtain the product. Microwave radiation is adopted for assisting enzymolysis, energy can be provided for an enzymolysis system, the temperature needed by enzymolysis can be maintained, the enzymolysis efficiency is greatly improved, the prepared sipunculus nudus peptide can stabilize iron ions, a safe and efficient iron supplementing preparation can be developed, meanwhile, reproductive function decline caused by obesity can be comprehensively repaired, the advantage of multi-dimensional conditioning is achieved, and the sipunculus nudus peptide has a good application prospect. And the method is suitable for metabolism-related reproductive disorder treatment.

The invention discloses a Karenia mikimotoi red tide biotoxicity and risk assessment method, and belongs to the field of marine environment monitoring and ecological risk assessment. The method comprises the steps of karenia mikimotoi culture, marine model fish model establishment, karenia mikimotoi exposure, multi-system toxicity index detection and data analysis. By integrating five toxic pathways (hemolysis injury, detoxification metabolism, oxidative stress, immune inflammation and reproductive function), systematic evaluation from a micromechanism (gene / cell) to a macroscopic effect (tissue / individual) is realized, and a toxic action mechanism is comprehensively revealed. By means of LDH, blood sugar, ROS and other sub-lethal indexes, early warning is achieved before irreversible injury occurs, and more abundant response time is provided for red tide disaster prevention. Traditional phenomenon observation is broken through, multi-system dysfunction caused by energy depletion, immune attack and other new mechanisms of Karenia mikimotoi is revealed through a key gene expression mode, and a new target is provided for toxicity prevention and control.

The invention discloses an application of curcumin in improving puberty male reproductive capacity damage caused by chemotherapeutic drugs. Aiming at the problems of testicular germ cell quantity reduction, oxidative stress injury, long-term fertility damage and the like caused by cytosine arabinoside chemotherapy, curcumin is applied while or before the cytosine arabinoside is applied. The application is realized through combined administration or a pharmaceutical composition containing curcumin and cytarabine. Experiments show that the scheme can effectively relieve testicular tissue form damage, reduce germ cell apoptosis and antagonize oxidative stress, and significantly improve the sperm quality of adult individuals. The invention provides a new potential strategy for synchronously protecting the reproductive function of a clinical child tumor patient during chemotherapy.

The invention relates to the technical field of peptide extraction, in particular to phascolosoma esculenta peptide and an extraction method and application thereof. According to the specific technical scheme, the method comprises the following steps: 1) crushing phascolosoma esculenta raw materials, adding water, adding sodium hydrogen sulfite and ascorbic acid, and stirring and soaking for 10-15 minutes; 2) grinding the soaked raw materials, and adjusting the pH value of the slurry to be alkaline; (3) adding alkali enzyme into the slurry, carrying out microwave enzymolysis for 45-50 minutes, adding papaya peptidase B, continuously carrying out microwave enzymolysis for 45-50 minutes, heating, inactivating, cooling, carrying out centrifugal filtration, and collecting filtrate; and 4) sequentially carrying out microfiltration, ultrafiltration and sodium filtration on the obtained filtrate to obtain the product. According to the phascolosoma esculenta peptide and the preparation method thereof, microwave radiation is adopted for assisting enzymolysis, energy can be provided for an enzymolysis system, the temperature needed by enzymolysis can be maintained, the enzymolysis efficiency is greatly improved, the prepared phascolosoma esculenta peptide can stabilize iron ions, a safe and efficient iron supplementing preparation can be developed, meanwhile, reproductive function decline caused by obesity can be comprehensively repaired, the advantage of multi-dimensional conditioning is achieved, and the phascolosoma esculenta peptide has a good application prospect. And the method is suitable for metabolism-related reproductive disorder treatment.

The invention belongs to the technical field of biological medicines, and particularly relates to application of Fatostatin in preparation of a medicine for preventing and / or treating male reproductive dysfunction. Through an orchitis mouse model, it is proved that Fatostatin can increase the testis weight of an orchitis mouse and significantly improve abnormal sperm quality and abnormal sperm number of the orchitis mouse. According to the application, the Fatostatin is used for preventing or treating the male reproductive dysfunction for the first time, an ideal treatment effect is achieved, a new prevention and treatment choice is provided for clinical application and treatment of male sterility, especially male sterility caused by inflammation infection, and the application has extremely high application value and social benefits.

This invention relates to the field of biotechnology, specifically to a hippocampal exosome-like vesicle, its preparation method, and its applications. This invention discloses for the first time a hippocampal exosome-like vesicle, which is a nanoscale membrane vesicle exhibiting a typical saucer-like morphology with an average particle size of less than 150 nm. It can be effectively taken up by mouse testicular interstitial cells, promoting testicular interstitial cell proliferation and sex hormone secretion. It can be used as a beneficial dietary supplement to improve male reproductive health and enhance reproductive function.

The application provides a traditional Chinese medicine composition for adjusting metabolism of polycystic ovary syndrome, a preparation and purposes, which is prepared from the following raw medicinal materials in a proportion by weight: Radix Rehmanniae Preparata 25-35 parts, Radix Paeoniae Alba 25-35 parts, Rhizoma Atractylodis Macrocephalae 25-35 parts, Radix Salviae Miltiorrhizae 25-35 parts, Radix Morindae Officinalis 25-35 parts, Fructus Corni 10-20 parts, Rhizoma Pinelliae Praeparatum 10-20 parts and Pericarpium Citri Reticulatae 10-20 parts. The eight kinds of traditional Chinese medicines are compatible with each other, and the effects of tonifying kidney and essence, invigorating spleen and drying dampness are achieved. The present application can reduce serum androgen, restore the level of estrus cycle, promote ovulation and effectively improve reproductive function. The clinical application practice shows that no intolerable side effect is found.

The invention discloses application of vincamine in preparation of a medicine for treating testis ischemia-reperfusion injury. Research results show that vincamine can significantly improve the pathological structure of the testis, alleviate inflammatory response and oxidative stress injury, and inhibit pyroptosis of testis cells, so that the reproductive function of the testis is protected after testis torsion reduction; the action mechanism is that an NF-kappa B signal channel is inhibited by up-regulating GPR40. The invention provides a novel potential drug for testis ischemia reperfusion injury.

The invention belongs to the technical field of biological medicines, and particularly relates to application of fumaric acid in preparation of a product for preventing, improving or treating male dyszoospermia. In a specific embodiment, the invention relates to application of fumaric acid in preparation of a product for preventing, improving or treating senile male dyszoospermia. In experiments, the inventor finds that fumaric acid can obviously improve the sperm concentration of old mice, improve the number of spermatogenic cells and improve the serum testosterone level of the old mice. The fumaric acid is used for treating the male reproductive dysfunction of the elderly for the first time, an ideal treatment effect is achieved, a new choice is provided for clinically treating the male reproductive dysfunction of the elderly, and the fumaric acid has extremely high application value and social benefits.

The invention relates to the technical field of biological medicines, in particular to an osteopontin probiotic composition and application thereof to improvement of male reproductive health. The invention provides an osteopontin probiotic composition which comprises probiotics and osteopontin, and the probiotics are bifidobacterium longum subsp. Infantis B8762; the osteopontin comprises a cow milk source. The probiotics and the osteopontin are combined for application, key reproductive genes in testis can be effectively adjusted, reproductive dysfunction is relieved, and male reproductive health is improved.

The embodiment of the invention discloses an intestinal probiotic combined preparation for relieving radiation injury and a preparation method thereof. The intestinal probiotic combined preparation comprises bifidobacterium longum, lactobacillus and streptococcus thermophilus, wherein the bifidobacterium longum is greater than or equal to 0.50 * 10 < 8 > CFU, the lactobacillus is greater than or equal to 0.50 * 10 < 8 > CFU, and the streptococcus thermophilus is greater than or equal to 0.50 * 10 < 8 > CFU. The preparation provided by the invention can regulate intestinal flora, enhance the barrier, comprehensively recover intestinal microecological balance and remarkably relieve injury caused by ionizing radiation, the survival rate of mice subjected to whole-body irradiation is increased, anxiety and depression emotions, the pulmonary respiratory function and the reproductive function are improved, and the preparation has a relatively good application prospect.

The invention discloses application of sodium 4-phenylbutyrate in a product for improving dyszoospermia of obese males, and belongs to the technical field of biological medicines. High-fat feed is adopted to construct a rat obesity model, the protection effect of 4-PBA on the reproductive function of obese rats is studied, and experimental results show that after the 4-PBA intervenes the obese rats, the testosterone content can be obviously increased, the estradiol level can be reduced, the sperm motility can be improved, the sperm malformation rate can be reduced, the spermatogenic dysfunction can be obviously relieved, and the anti-obesity effect on testis tissue morphology, testis tissue morphology, testis tissue morphology, testis tissue morphology and testis tissue morphology can be improved. The functions of supporting cell mitochondria, endoplasmic reticulum and MAM structures are obviously improved, and a new treatment strategy and a new drug target are provided for intervening and controlling obesity induced testis dyszoospermia and improving fertility of obese males.

The invention belongs to the field of biological medicine, and particularly discloses application of carnosine injection in improving fertility of elderly female mammals. The injection comprises carnosine and normal saline, the preferable concentration is 25 mg / mL, the injection is administrated through abdominal cavity or subcutaneous injection, the single dose is 0.125 mg / g body weight, and the injection is administrated once every 5 days. The carnosine injection increases the number of ovary cavity follicles by repairing the mitochondrial morphology of oocyte (reducing swelling and clearing the ridge structure), improves the blastocyst formation rate of fertilized ovum, and systematically improves the reproductive function of elderly individuals. Animal experiments prove that supplement of carnosine can significantly improve blastocyst quality, embryonic development potential and litter size of elderly mice, is non-toxic to maternal tissues, and does not affect growth and development of offspring. The invention provides an intervention scheme of the carnosine injection aiming at the aging fertility decline for the first time, has the advantages of clear mechanism, high safety, simplicity and convenience in operation and the like, and provides an innovative solution for the aging female reproductive health problem.

The invention discloses a group of isoprostaglandin metabolite markers for diagnosing male offspring reproductive function damage caused by exposure of nano-plastics during pregnancy and application of the isoprostaglandin metabolite markers. According to the present invention, it is found that after the parental generation is exposed to the nanometer plastic, the expression level of the prostate hormone metabolism marker 8-iso PGF3 [alpha] and / or 8-is-13, 14-dihydro-15-keto-PGF2 [alpha] related to reproductive injury in the male offspring is significantly up-regulated; therefore, 8-iso PGF3 alpha and / or 8-iso-13, 14-dihydro-15-keto-PGF2 alpha are / is taken as the metabolic marker, the influence of the male offspring reproductive injury caused by the nano plastic is disclosed, and a method is provided for diagnosis and evaluation of the male offspring reproductive injury caused by the nano plastic.

The invention belongs to the field of medicines for treating male infertility diseases, and discloses application of 12-ketolithocholic acid (12-keto-LCA) in the aspect of improving sperm quality decline, namely application of 12-keto-LCA in preparation of medicines for improving sperm quality decline. The invention provides a new application of 12-keto-LCA, reveals for the first time that 12-keto-LCA can support the spermatogenesis process, especially can improve the reproductive function of obese males, realizes the effects of improving testosterone secretion, repairing the spermatogenic function, improving the sperm quality and embryonic developmental ability and the like, and improves the condition that the sperm quality of mammals is reduced. The 12-keto-LCA can effectively relieve dyszoospermia of males, especially relieve damage of obesity to the reproductive function of males, and a new way is provided for developing drugs for improving the reproductive function of obese males.

This invention discloses the application of the compound pimozide in combination with metronidazole in the preparation of a drug for improving oocyte quality and treating endometriosis-related fertility disorders, belonging to the field of biomedical technology. The efficacy of this invention was verified by constructing mouse models of endometriosis driven by pathogenic *Prevotella bisporus* and mouse models of ovarian endometriosis. Experimental results showed that the combined administration significantly eliminated *Prevotella bisporus* colonizing ectopic lesions, reduced succinic acid content in both eutopic and ectopic lesions, decreased the succinylation level of lysine residue 195 of the ACADVL protein, reduced the fluidity of primary endometrial epithelial cell membranes, reduced the volume and weight of endometriotic lesions, and improved oocyte spermatogenesis, ovulation rate, and litter size. This invention solves the problem that existing therapies cannot simultaneously control endometriosis lesions and protect reproductive function, providing a new, safe, and effective combined treatment option for patients with endometriosis and infertility.

The invention belongs to the field of traditional Chinese medicines and medical health-care foods, and particularly relates to a medicinal and edible traditional Chinese medicine composition for improving intestinal flora and contributing to improving ovarian functions and a preparation process and application thereof, and the composition comprises five parts: an energy supply part, a medicinal and edible traditional Chinese medicine part, a dietary fiber part, a vitamin part and a mineral part. Big data analysis on traditional Chinese medicines of the food composition discovers that the food composition has an effect of assisting reproduction, and related animal experiments prove that the food composition has the effects of improving the ovarian function, regulating serum sex hormone and increasing the pregnancy rate; the relative abundance of bacteroides can be remarkably improved, and the relative abundance of firmichelia is reduced, so that the effect of improving intestinal flora is achieved. Various medicinal and edible traditional Chinese medicines are creatively combined, energy supply, vitamins, mineral substances and dietary fibers are added at the same time, traditional Chinese medicine theories and dialectical treatment are organically fused with modern nutriology, the reproductive function is effectively improved, intestinal flora is adjusted, and nutrients required by pregnancy preparation, pregnancy and lactation are supplemented.

Methods of preventing or restoring age-related loss of reproductive function in a mammal by contacting germ cells of the mammal with a composition comprising a flavonoid glycoside (e.g., rutin) and L-arginine are provided. Also provided are methods of preventing or restoring age-related reproductive function loss, preventing or restoring age-related fertility decline, and reducing the risk of age-related reproductive function loss by administering the composition to a mammal. Also disclosed is the use of the composition for preventing or restoring age-related fertility decline in a mammal.

The invention provides application of alpha-ketoglutaric acid in preparation of a medicine for regulating and controlling the number and differentiation of primordial germ cells, and relates to the technical field of biological medicine. The invention finds that alpha-ketoglutaric acid can obviously promote proliferation of primordial germ cells in the key stage of embryonic development, and more importantly, the alpha-ketoglutaric acid can obviously delay the differentiation of the primordial germ cells and the start of meiosis in the key stage of embryonic development. On the basis of providing more sufficient germ cell reserve for individuals, the expansible state of the individuals is prolonged, so that the individuals have the potential advantage of maintaining the subsequent reproductive function physiologically. The alpha-ketoglutaric acid disclosed by the invention provides a new application value for the alpha-ketoglutaric acid in the aspects of reproductive health maintenance and fertility support.

The invention belongs to the technical field of traditional Chinese medicine preparations, and particularly relates to a traditional Chinese medicine effervescent tablet for kidney yin deficiency and fire hyperactivity type reproductive function decline and a preparation process thereof. According to the traditional Chinese medicine effervescent tablet, the prescription and the preparation method are optimized, the problem of loss of effective components of traditional Chinese medicines caused by an acid source and an alkali source is solved, and the traditional Chinese medicine effervescent tablet which can repair germ cells and promote reproductive capacity and is high in effective component content is provided.