15 results about "Testicular Interstitial Cells" patented technology

The invention relates to the technical field of biological medicine, and discloses rapamycin-loaded microemulsion gel as well as a preparation method and application thereof. The preparation method of the microemulsion gel comprises the following steps: dissolving N, N-dimethyl octadecyl carboxymethyl chitosan in a water phase, then adding an oil phase, a cosurfactant and rapamycin, and stirring to obtain rapamycin-loaded microemulsion; the preparation method comprises the following steps: dissolving a high polymer material in a PBS solution, heating until the high polymer material is completely dissolved, then adding dopamine, stirring for reaction, and finally dialyzing and freeze-drying to obtain the adhesive. And adding a gel matrix containing a thickening agent and an adhesive into the microemulsion, and stirring to obtain the rapamycin-loaded microemulsion gel. According to the prepared microemulsion gel, transdermal drug delivery of hydrophobic drugs is achieved, testis are coated with the microemulsion gel loaded with rapamycin, the blocking effect of damp-heat stress on differentiation and development of testis interstitial cells can be relieved, and the functionality of a male reproduction endocrine system is recovered.

The application belongs to the field of regenerative medicine and nano drug delivery system, and particularly relates to a drug-loaded exosome for improving the testosterone secretion capacity of testicular interstitial cells and a preparation method and application thereof. The application links a targeting FATE1 polypeptide to the surface of MSC-derived exosomes by a chemical coupling method, and loads a small molecule compound in the exosomes by electroporation, so as to obtain modified MSC-derived exosomes. The obtained exosomes can obtain the targeting capacity in testicular interstitial cells, and the natural repair capacity of MSC-derived exosomes and the release of small molecule drugs promote the repair of damaged cells, improve the testosterone secretion capacity of testicular interstitial cells, and relieve male erectile dysfunction caused by diseases or aging.

The invention relates to the technical field of genetic engineering, in particular to application of PCK1 in preparation of products for regulating and controlling goat testicular interstitial cell proliferation. According to the invention, an over-expression plasmid pcDNA3.1 (+)-PCK1 of the PCK1 gene is constructed, an interference sequence si-PCK1 of the PCK1 gene is synthesized, and it is proposed for the first time that the over-expression of the PCK1 gene can significantly promote the proliferation of goat testicular interstitial cells, and the inhibition of the expression of the PCK1 gene can significantly inhibit the proliferation of the goat testicular interstitial cells. The invention has important significance for improving the development of the goat testis, and provides technical support for researching and developing related products for regulating and controlling the proliferation of the goat testis interstitial cells by taking the PCK1 gene as the target spot.

The invention relates to the technical field of genetic engineering, in particular to application of miR-145-3p as a target spot in preparation of products for regulating and controlling proliferation of goat testicular interstitial cells. According to the invention, Inhibitor and Mimics sequences taking the miR-145-3p gene as a target spot are constructed, and it is proposed for the first time that overexpression of the miR-145-3p gene can significantly inhibit proliferation of goat testicular interstitial cells, and inhibition of the miR-145-3p gene can significantly promote proliferation of goat testicular interstitial cells. The invention has important significance for improving the development of the goat testis, and provides technical support for researching and developing related products for regulating and controlling the proliferation of the goat testis interstitial cells by taking the miR-145-3p gene as a target spot.

The invention relates to the technical field of biological medicines, and discloses application of rapamycin in preparation of a medicine for protecting male reproductive function from damp-heat stress injury. The invention also discloses application of the rapamycin derivative in preparation of a medicine for protecting male reproductive function from damp-heat stress injury. The rapamycin derivative is everolimus, temsirolimus or defolimus. The invention discloses the new application of the rapamycin in preparing the medicine for treating the damp-heat stress caused by the high-temperature and high-humidity environment for the first time, and the action mechanism of the rapamycin is that the rapamycin inhibits an mTOR (mammalian Target of Rapamycin) pathway and activates cell protective autophagy. Animal experiments show that the rapamycin can significantly improve the testosterone concentration of a damp-heat stress model, effectively recover the protein expression of PDGFRalpha and 3beta-HSD, reverse the development process of damaged testicular interstitial cells, and provide a brand new drug choice with a clear action mechanism for preventing and treating the influence of damp-heat stress on a male reproductive endocrine system.

The present application relates to the technical field of genetic engineering, and particularly relates to application of miR-27b-3p in preparation of products for regulating cell proliferation and testosterone synthesis. The present application constructs Inhibitor and Mimics sequences with miR-27b-3p gene as a target, and for the first time proposes that overexpression of miR-27b-3p gene can significantly inhibit proliferation and testosterone synthesis of goat testicular interstitial cells, and inhibition of miR-27b-3p gene can significantly promote proliferation and testosterone synthesis of goat testicular interstitial cells. The present application has important significance for improving development of goat testis, and provides technical support for research and development of products for regulating proliferation and testosterone synthesis of goat testicular interstitial cells with miR-27b-3p gene as a target.

The application belongs to the technical field of reproductive health, and particularly relates to a new application of a testicular metabolite 2-n-propylthiazolidine-4-carboxylic acid (PTCA), and the 2-n-propylthiazolidine-4-carboxylic acid is used for preparing a drug for treating testicular spermatogenic disorder induced by AFB1. The PTCA can promote the secretion of testosterone of testicular interstitial cells, activate the PI3K / AKT / NRF2 antioxidant signaling pathway in testicular tissue, relieve testicular injury induced by AFB1, and reduce the ROS level in testicular tissue. The application discloses, for the first time, the important role of the PTCA as a new testicular metabolic marker and protective agent in the male reproductive system, provides a new insight into the reproductive toxicity mechanism of AFB1, and lays an experimental foundation for the application of metabolites to the intervention strategy of male spermatogenic disorder.

The invention relates to application of ginsenoside Rg4 as a unique active ingredient in preparation of a medicine for improving male delayed gonad hypofunction, belonging to the field of biological medicine. According to the invention, the action mechanism of the Rg4 for improving LOH is defined for the first time: the Rg4 can specifically up-regulate the mRNA expression quantity of StAR and StARD7 in a testis tissue, and promote the transport of cholesterol to the inner mitochondrial membrane of a testis Leydig cell from the transcriptional level; the Rg4 improves the mRNA expression quantity of P450scc, 3 beta-HSD in testicular tissues by promoting the efficient transport of cholesterol, enhances the conversion of cholesterol to pregnenolone and the catalytic synthesis of subsequent steroids, and improves the endogenous testosterone synthesis capability; the Rg4 can recover the negative feedback regulation function of an HPG axis, regulate secretion of pituitary LH and FSH to physiological steady state, and avoid abnormal fluctuation of hormone level. The invention fills the technical blank of Rg4 in the field of LOH gene regulation and control.

This invention relates to the field of biotechnology, specifically to a hippocampal exosome-like vesicle, its preparation method, and its applications. This invention discloses for the first time a hippocampal exosome-like vesicle, which is a nanoscale membrane vesicle exhibiting a typical saucer-like morphology with an average particle size of less than 150 nm. It can be effectively taken up by mouse testicular interstitial cells, promoting testicular interstitial cell proliferation and sex hormone secretion. It can be used as a beneficial dietary supplement to improve male reproductive health and enhance reproductive function.

The present application relates to the technical field of genetic engineering, and particularly relates to application of PPARG in preparation of products for regulating cell proliferation and testosterone synthesis.The present application constructs a super-expression plasmid pcDNA3.1(+)‑PPARG of PPARG gene, simultaneously synthesizes an interference sequence si‑PPARG, and for the first time proposes that overexpression of the PPARG gene can significantly promote proliferation of goat testicular interstitial cells and testosterone synthesis, and inhibition of the PPARG gene can significantly inhibit proliferation of goat testicular interstitial cells and testosterone synthesis.The present application has important significance for improving development of goat testis, and provides technical support for research and development of products for regulating goat testicular interstitial cell proliferation and testosterone synthesis by taking the PPARG gene as a target.

The invention belongs to the technical field of construction of cell models, and particularly relates to a method for constructing a hyperuricemia testicular interstitial cell model. Comprising the following steps: (1) selecting an 8-week-old male C57BL / 6 mouse, dissecting, collecting testis, removing testis albuginea, cutting into fragments, digesting by utilizing collagenase IV, filtering, centrifuging, discarding supernatant, diluting cell suspension, adding luteinizing hormone receptor coupled fluorescein to mark testis interstitial cells, and sorting by utilizing flow cytometry to obtain testis interstitial cell suspension; (2) counting by using a blood cell counting plate, diluting the testicular interstitial cells, inoculating the testicular interstitial cells into a 24-pore plate, and culturing the testicular interstitial cells in a cell culture box; and (3) after 24-48 hours, taking out the 24-well plate, discarding cell supernate, cleaning with PBS, adding a uric acid solution, and continuously culturing to obtain the hyperuricemia testicular interstitial cell model. The obtained interstitial cell model can be used for exploring various function change mechanisms of the testicular interstitial cells in a hyperuricemia environment, and is simple to operate, effective, high in repeatability and short in time consumption.

This invention provides a method for isolating and identifying interstitial cells from Bactrian camels, belonging to the field of cell isolation technology. A mixture of 0.08–0.12% collagenase IV solution and 0.23–0.27% trypsin-EDTA at a volume ratio of 1:2 is used to digest Bactrian camel testicular tissue blocks. The mixture is then purified by hypotonic treatment to remove excess germ cells, differential adhesion purification to remove a large number of supporting cells, and Percoll density gradient centrifugation to remove a small number of supporting cells. The suspension, obtained from a 34%–60% Percoll gradient, is transferred to a new culture flask to obtain interstitial cells with a purity ≥95%. Identification is performed using morphological observation, immunofluorescence staining, and secretory function testing. The interstitial cells isolated from Bactrian camels using this invention can be used for research on the reproductive performance of Bactrian camels, laying the foundation for understanding the reproductive performance of Bactrian camels.

The present application belongs to the technical field of biological medicine, and particularly relates to a composition for treating male erectile dysfunction and application thereof. The oyster polypeptide is prepared by oyster protease hydrolysis, and the oyster polypeptide can effectively promote the proliferation of testicular interstitial cells, enhance and improve sexual desire and sexual behavior, has certain clinical application value, and has the advantages of simple preparation, easy absorption and the like, and is easy to popularize and apply.

The invention discloses a parent osteoarthritis intervention target based on an INSL3 / RXFP2 signal channel and application of the parent osteoarthritis intervention target. The pregestation caffeine exposure of the male body has obvious influence on the cartilage development of the male offspring, and the susceptibility of osteoarthritis is increased. Through screening and verification, INSL3 secreted by testicular interstitial cells plays a key role in the parent osteoarthritis, and cAMP / PKA / CREB signal channels are reduced by locally knocking out cartilage RXFP2 or inhibiting combination of INSL3 and RXFP2, so that synthesis and development of cartilage matrixes are inhibited. And the exogenous recombinant INSL3 protein is injected into the knee joint cavity, so that the cartilage dysplasia caused by pregestation caffeine exposure of the parent can be obviously improved. The invention discloses a key effect of an INSL3 / RXFP2 signal channel in regulation and control of cartilage matrix synthesis, and provides a new biological basis and an early prevention and treatment strategy for targeted therapy of osteoarthritis.

The invention relates to the technical field of biological medicine, in particular to application of combination of beta-nicotinamide mononucleotide (NMN) and apigenin (APG) in preparation of a product for delaying senescence of testicular interstitial cells. Compared with single treatment, the combined use of NMN and apigenin can significantly improve the NAD < + > level of aged mice, promote the expression of testosterone synthesis related genes and proteins, promote the synthesis of testosterone in testicular interstitial cells, enhance the cell proliferation activity, and improve the mitochondrial function and antioxidant defense system, thereby delaying the aging of the testicular interstitial cells. The discovery of the invention not only contributes to understanding the regulation mechanism of testosterone synthesis in the aging process, but also provides technical support for developing a new anti-aging strategy.