4472results about How to "Promote secretion" patented technology

The invention relates to a hand-mouth baby wet tissue and a preparation method thereof. The hand-mouth baby wet tissue is composed of compound wet tissue liquid and non-woven fabrics for holding the compound wet tissue liquid, wherein every 100 parts of the compound wet tissue liquid comprises the following components in percent by weight: 0.01%-7.5% of a sweetening agent, 0.01-2.0% of a preservative, 0.01%-2.0% of a cleaning agent, 0.05%-5.0% of a solubilizer, 0-10.0% of a humectant, 0-5.0% of a skin care agent, 0-5.0% of an acidity regulator, 0-5.0% of essential oil, 0-5.0% of a stabilizer, 0-1.0% of natural essence, and the balance of pure water. The hand-mouth baby wet tissue has the advantages that edible sweetening agent is added into the compound wet tissue liquid to promote spittle secretion of babies, relieve the drop of pH value in the oral cavity and prevent the formation of decayed teeth. The product formula is stable and safe, and the material components are non-toxic. The formula is safe, non-irritant and non-corrosive, so that the allergic risks are reduced to be the lowest. The hand-mouth baby wet tissue can be used for cleaning the hands and mouths of an infant, infant tableware and other infant supplies and toys.

The present invention relates to a formulation of herbal toothpowder or toothpaste for gums and teeth, which comprises of powder or paste of Zanthoxylum armatum (20-25%), Zingiber officinale (25-30%), Santalum album (8.25-8.5%), Spilanthes calva (2.0-2.5%), Pistacia lentiseus (2.0-2.5%), Quercus infectoria (8.0-8.5%), Usnea longissima (1-4%), as well as roasted alum and common salt.

The invention discloses a CAR-T transgene vector based on replication defective recombinant lentivirus. The CAR-T transgene vector comprises an original nuclear replicon pUCOri sequence, a resistance gene AmpR sequence containing ampicillin, a virus replicon SV40 Ori sequence, a lentivirus packaging cis element, ZsGreen1 green fluorescent protein, an IRES ribosome binding sequence, a human EF1 alpha promoter , a chimeric antigen receptor of second-generation CAR or third-generation CAR and a regulating element, wherein the original nuclear replicon pUCOri sequence is used for plasmid replication; the resistance gene AmpR sequence is used for massively proliferating target strains; the virus replicon SV40 Ori sequence is used for enhancing replication in eukaryocyte; the lentivirus packaging cis element is used for lentivirus packaging; the ZsGreen1 green fluorescent protein is used for expressing green fluorescent for eukaryocyte; the IRES ribosome binding sequence is used for jointly transcribing and expressing protein; the human EF1 alpha promoter is used for conducting eukaryotic transcription on antigen receptor genes; the chimeric antigen receptor is used for forming the second-generation CAR or the third-generation CAR integrating recognition, transfer and start; the regulating element is used for enhancing expression efficiency of transgenes and used after eWPRE-enhanced type woodchuck hepatitis b virus is transcribed. Besides, the invention further discloses a construction method and application of the vector. By means of the CAR-T transgene vector and the construction method and application of the vector, secretion of cell factors and an in vitro killing effect of CAR-T cells can be remarkably improved, and the clinical treatment effect is remarkable.

The present invention relates to novel antibodies capable of binding specifically to the human insulin-like growth factor I receptor IGF-IR and / or capable of specifically inhibiting the tyrosine kinase activity of said IGF-IR receptor, especially monoclonal antibodies of murine, chimeric and humanized origin, as well as the amino acid and nucleic acid sequences coding for these antibodies. The invention likewise comprises the use of these antibodies as a medicament for the prophylactic and / or therapeutic treatment of cancers overexpressing IGF-IR or any pathology connected with the overexpression of said receptor as well as in processes or kits for diagnosis of illnesses connected with the overexpression of the IGF-IR receptor. The invention finally comprises products and / or compositions comprising such antibodies in combination with anti-EGFR antibodies and / or compounds and / or anti-cancer agents or agents conjugated with toxins and their use for the prevention and / or the treatment of certain cancers.

The invention relates to natural attractants, belonging to the field of feed additives. One natural attractant is mainly composed of a dried tangerine peel crushed substance, a hawthorn crushed substance, a cinnamon crushed substance, a mugwort leaf crushed substance, and composite microbial powder. The other natural attractant is mainly composed of a dried tangerine peel extractive, a hawthorn extractive, a cinnamon extractive, a mugwort leaf extractive, and composite microbial powder. The attractants provided by the invention has the advantages of promoting the ingestion of livestock, poultry and aquatic animals, increasing the ingestion speed and feed intake of the livestock, poultry and aquatic animals, avoiding the indigestion caused by overfeeding, increasing the feed intake and digestibility of the animals to the feeds hard to digest, expanding the application range and usage amount of unconventional feed materials, enhancing the immunity and anti-stress capability of the animals, resisting diseases, promoting growth, decreasing the production cost of the animal feeds and increasing the production benefits; the attractants contain no antibiotic and chemical medicine as well as drug residue, can be used for a long time without causing drug resistance, and have safety and high efficiency.

The invention discloses exendin-4, an analog fusion protein thereof, the corresponding polynucleotide sequence, carrier, host cell and pharmaceutical composite and a preparation method and applications of the fusion protein. The fusion protein is prepared by fusing exendin-4 and analog thereof with human immunoglobulin IgG2-Fc through special connecting peptide and has better stability and loner half-life in vivo. The fusion protein can be administered by performing local delivery, using aerosol and using injection. The fusion protein can promote the regeneration and repair of islet beta cells, increase islet beta cells, promote the secretion of insulin and improve the sensitivity of organism to insulin. The fusion protein is used to cure diabetes, adiposity and other diseases which can be benefited by reducing plasma glucose and inhibiting gastrointestinal motility and gastric emptying.

A high-activity composite microbial product for ensilage is composed of the streptococcus lactis and other 3 bacterial strains. After it is added to the raw materials of ensilage, such as corn plant, alfalfa, straw, stalk and pasturage, their pH value can be quickly lowered and the cellulose and semi-cellulose can be decomposed to obtain excellent feed.

The invention generally relates to a bone growth factor, and more particularly to compositions including NELL1, articles of manufacture including NELL1 and methods of using NELL1 to induce bone formation. This invention also provides methods for the expression and purification of NELL1 and NELL2 peptides.

The instant invention provides for proteins, polypeptides, nucleic acid sequences, constructs, expression vectors, host cells, pharmaceutical compositions of, and methods for using human placental bikunin, serine protease inhibitor domains, and fragments thereof

The invention discloses rose vinegar. A process method of the rose vinegar includes: subjecting roses serving as raw materials to dry crushing, extraction by edible alcohol, alcoholic strength adjustment, acetic fermentation, clarifying, ageing, filtering, mixing, filling and sterilization so that a rose vinegar finished product is obtained. The rose vinegar fully retains nutrition and efficacy of the roses, is rich in multiple amino acids, vitamins, mineral substances and antioxidant components of polyphenol, flavone and the like, and has the efficacy of detoxification, keeping young, promoting qi to activate blood, inducing resuscitation, removing stasis, soothing the liver, refreshing the mind, promoting biliation, easing digestion and regulating mechanism. The rose vinegar is free from addition of any spice and chemical component, rich in nutrition, sweet in aroma and suitable for ladies to drink and has the efficacy of maintaining beauty and keeping young.

A breeding-pig compound premix comprises 0.6-1% of vitamin additive, 3.8-6.3% of trace mineral additive, 4-6% of amino acid additive, 0.5-0.8% of functional additive, 10-12% of flavor, 0.1-0.3% of antioxidant, 2.5-4% of choline chloride, 30-40% of dicalcium phosphate, 30-40% of limestone powder, as well as 2-10% of feed carrier. The adding amount of the premix accounts for 4%; the premix is coupled with the common grain crops, such as corn and soybean meal, and uniformly mixed into full-price formula feed for barren sows, so that the barren sows can timely get rutted and mated to discharge more eggs and produce more litter; therefore, the premix improves the breeding performance of the sows and enhance the economic benefits of the hogpen.

Novel enterokinase cleavage sequences are provided. Also disclosed are methods for the rapid isolation of a protein of interest present in a fusion protein construct including a novel enterokinase cleavage sequence of the present invention and a ligand recognition sequence for capturing the fusion construct on a solid substrate. Preferred embodiments of the present invention show rates of cleavage up to thirty times that of the known enterokinase cleavage substrate (Asp)4-Lys-Ile.

The present invention provides materials and methods relating to mildly polar fluid extracts of plant materials, such as Artemisia plant species, useful in methods for treating diabetes and methods for modulating the activity of glucagon-like peptide-1 (GLP-1), and in methods for modulating phosphoenol pyruvate carboxykinase (PEPCK) activity in a diabetes-specific manner. The extracts are generally non-toxic and non-mutagenic and may be administered to diabetics with beneficial effect on blood glucose levels. The extracts may also be administered to non-diabetics without deleterious effect. The plants are easily grown with a minimum of time, labor, and cost. Extracts are inexpensively and quickly prepared without the need for fractionation to remove potentially deleterious compounds, and the extracts may be administered to mammals such as humans through various routes, in a variety of forms, and at convenient concentrations.

The invention discloses a green health-preservation sweet-scented osmanthus tea beverage. The green health-preservation sweet-scented osmanthus tea beverage comprises the following raw materials in parts by weight: 30-40 parts of sweet-scented osmanthus, 1-2 parts of honey, 3-5 parts of Chinese wolfberry fruits, 7-11 parts of fresh tea leaves, 2-3 parts of haws, 3-5 parts of sugarcane, 2-4 parts of cucumbers,3-5 parts of aloe, 0.08-0.14 part of a thickening agent, 0.01-0.03 part of an antioxidant stabilizing agent, 0.1-0.3 part of a vitamin additive and 12-16 parts of distilled water. The sweet-scented osmanthus tea beverage disclosed by the invention has the functions of clearing heat, removing toxicity, moisturizing the intestines, regulating the function of the stomach, regulating vital energy and nourishing the lungs, and is mellow in mouth feel, moderate in sour and sweet degrees, delicious, pleasant in delicate fragrance, high in nutrient value; in addition, the beverage does not precipitate and is good in color singleness; and besides, through the preparation method of the green health-preservation sweet-scented osmanthus tea beverage disclosed by the invention, sweet-scented osmanthus blooming seasonal restrictions are avoided, the green health-preservation sweet-scented osmanthus tea beverage can be processed in an all-weather manner, the raw materials are non-toxic, harmless and convenient to obtain, and the green health-preservation sweet-scented osmanthus tea beverage is low in cost and simple in technology and has higher use value and favorable application prospects.

The invention discloses a method for making fermented surimi by utilizing a lactic acid bacteria starter, and the method is characterized by adopting fresh or frozen fish as the raw material and comprising the steps of preprocessing, chopping, inoculating the starter, filling, fermenting and storing, thus obtaining the surimi. The product processed in the method has fresh taste, good gel property, intense fermentation flavor, high nutritive value and good safety and can solve the problems that protein of freshwater fish is easy to modify when being frozen and has poor gel strength and heavy fishy smell, and the method has simple process, greatly enhances the yield rate and the gel strength of the product and improves the flavor of surimi products.

The present invention relates to a kind of Chinese medicine composition for preventing bird flu of chicken and duck. The Chinese medicine composition is prepared with forsythia 90-110 weight portions, honeysuckle 40-60 weight portions, northern isatis root 25-45 weight portions, skullcap root 40-60 weight portions, basket fern rhizome 40-60 weight portions and astragalus root 70-90 weight portions. The Chinese medicine composition is for fowls to take orally, has obvious bird flu preventing effect and is safe and convenient.

The invention provides human umbilical cord mesenchymal stem cell exosome type external cream and a preparation method thereof, and aims at the solving the problem in effectively inducing human umbilical cord mesenchymal stem cells to massively synthesize and secrete mesenchymal stem cell exosome capable of specifically promoting epidermis cell and hypodermal cell to proliferate, divide and differentiate, promoting the formation of collagenous fibers, reticular fibers and elastic fibers, and adjusting the secreting functions of collagen and mucopolysaccharide. The cream comprises human umbilical cord mesenchymal stem cell exosome extracting liquid and a base cream formula based on the mass ratio ranging from (1:20) to (3:10), wherein the human umbilical cord mesenchymal stem cell exosome extracting liquid is prepared by performing hungry culture on P2-P5 generations of human umbilical cord mesenchymal stem cells and stimulating through epidermal growth factors. The prepared cream has the effects of improving the skin quality, resisting the aging of skin cells, recovering the normal structure and physiological function of the skin, reducing the skin atrophy exsiccosis, and moisturizing the skin tissue.

The present invention relates to a cosmetic product with dispelling striae gravidarum effectiveness and a manufacturing method. Nutrient components synergy and can repair the elastic fiber effectively. Sweet almond oil and olive oil in the formula are rich in unsaturated fatty acid, which is totally identical with fats secretory of human; so essential fatty acid is added; the skin is repaired. Vitamin E has anti-aging and antioxidant effectiveness; small molecule faba bean peptide and nanometer parcel epidermal growth factor biomimetic peptide have strong physiological excitation signal which is easily absorbed by the skin; so the skin is stimulated to compose collagen which markedly amends the striae gravidarum. Centella asiatica extract can promote the composition of collagen I and III and can promote the composition of polysaccharide, which effective prevents and repairs the striae gravidarum.

The invention discloses a Chinese medicinal composition for improving the egg yield of laying hens and a feed. The Chinese medicinal composition comprises astragalus root, Eucommia ulmoides, honeysuckle, pilose asiabell root, sanguisorba officinalis, hawthorn, tuckahoe, Mangnolia officinalis, Chinese pulsatilla root, giant knotweed, ricepaper pith, bighead atractylodes rhizome, dried tangerine peel, malt, isatis root, atractylodes rhizome, indigowoad leaf, licorice, cyrtomium fortunei, nutgrass galingale rhizome, grassleaf sweelflag rhizome, pinellia tuber, medicated leaven, Chinese angelica and root of rehmannia. According to the composition, through compatibility of the medicated leaven and the hawthorn, the digestion function of the laying hens is enhanced, and the nutrient absorption rate of the laying hens is improved; and through compatibility of the Chinese angelica and the root of rehmannia, secretion of yolk of the laying hens is promoted, so that the egg yield is improved.

A Chinese medicine for preventing and treating cholecystitis, cholelithiasis and the inflammation of biliary tract, and improving the function of gallbladder and liver is prepared from 23 Chinese-medicinal materials including tackahoe, capejasmine fruit, white peony root, cyprus tuber, etc.

The invention discloses a method for simultaneous and efficient amplification of CD<3+>CD<56+>CIK cells and CD<3->CD<56+>NK cells. The method comprises the steps as follows: the concentration of separated PBMC (peripheral blood mononuclear cells) is adjusted by a serum-free medium containing autologous plasma, an Anti-CD16 antibody, IL-2 and IL-15 are added, and then the mixture is transferred into a T175 culture flask for culture; an Anti-CD3 antibody and an Anti-CD137 antibody are added; a serum-free medium containing the autologous plasma, IL-2 and IL-15 is supplemented every two days according to the cell growth condition; the cell concentration is controlled to be about 1.5*10<6> / ml; and after culture is performed for 14-21 days, large quantities of high-purity CD<3+>CD<56+>CIK cells and CD<3->CD<56+>NK cells can be obtained simultaneously, and the total cell quantity can reach an effective value of the cell quantity required for adoptive cellular immunotherapy clinically for tumor. The method for simultaneous and efficient amplification of the CD<3+>CD<56+>CIK cells and the CD<3->CD<56+>NK cells is simple, convenient, effective and high in cell killing activity.

The invention discloses an enteromorpha polysaccharide having immunocompetence and a preparation method thereof. The enteromorpha polysaccharide having immunocompetence is a polysaccharide HT-II. The enteromorpha polysaccharide having immunocompetence is a white floccule, can be well dissolved in water and dimethyl sulfoxide, has relative molecular weight of 96kDa, and mainly comprises glucose and mannose, wherein a ratio of rhamnoside to xylose to mannoside to glucose is 36: 20: 6: 38. The preparation method comprises the following steps of breaking down, carrying out extraction at a temperature of 90 DEG C by water, filtrating, merging the extract, concentrating, carrying out precipitation by ethanol, removing proteins, carrying out separation and purification, and carrying out freeze drying to obtain the purified enteromorpha polysaccharide having immunocompetence. The preparation method is simple, has stable performances, a low cost and a large medical value, can reduce cholesterol content and can improve immunity. The preparation method adopts extraction, protein removal and purification processes, and scientific experimental means, is a novel method for controlling enteromorpha, and provides a novel approach for controlling enteromorpha. The preparation method of the enteromorpha polysaccharide having immunocompetence develops enteromorpha medical values and purposes, controls environmental pollution, reduces a clean-up cost, improves economic benefits and is an ideal special preparation technology for enteromorpha control and integrated utilization.

The invention relates to a method and device for preparing bacterial cellulose composite materials quickly on a large scale. The method comprises the steps as follows: (1) a bacterial cellulose producing strain is inoculated in liquid medium for propagation and then transferred into a biological reactor containing framework material for perturbation cultivation, so that bacterial cellulose composite material can be obtained; and (2) bacterial cellulose of the bacterial cellulose composite material is peeled off from the framework material or the cellulose composite material is soaked in NaOH solution to be washed. The device comprises a thermometer opening, an acid liquor and alkali liquor feeding opening, a handle, an air vent, a pH meter opening, a nutrition feeding opening, a rotating shaft, a fermentation tank, a motor, a rotating drum, a fermentation tank jacket, a circulating water inlet, a circulating water outlet, a nutrient fluid inlet, a nutrient fluid outlet and a condensed water outlet. The rotating drum is fixed on the rotating shaft in the fermentation tank. The device provided by the invention has the advantages of simplicity, convenience, detachable die, low cost, high production efficiency and high degree of automation; and the obtained bacterial cellulose composite material can be widely applied.

The invention discloses a concentrated feed containing Chinese herbal medicine for growing-finishing pig. The concentrated feed comprises soybean meal, fish meal, rapeseed meal, stone powder, calcium hydrogen phosphate, soybean oil, and premix; the following Chinese herbal medicine components are added into the concentrated feed: rhizoma atractylodis, processed fleece-flower root, gentian, tangerine peel, white peony root, parched medicated leaven, parched hawthorn, parched malt, medulla tetrapanacis, semen cuscutae, stir-fried betelnut, radix astragali, scutellaria, rhubarb powder, etc. The feed can improve the immunity, disease resistance, and stress resistance of growing-finishing pigs; the feed has improved quality, can enhance the appetite of the animals, and promote the digestion and the absorption; and the utilization rate of the feed is increased, and the breeding cost is reduced. The feed also has blood circulation invigorating, stomach-strengthening, parasite-expelling, and rapid fattening functions.

The invention relates to fermentation waste vegetables feed and a preparation method. The fermentation waste vegetables feed consists of mixed raw materials and microorganism living bacteria liquid, wherein the mixed raw materials consist of vegetable waste materials, straw and corn powder; and each ton of the mixed raw materials are formed according to the weight ratio (w / w): 70 to 80 percent of waste vegetables, 20 to 25 percent of straw, and 4 to 6 percent of corn powder. Each ton of the mixed raw materials are added with 30 to 40 milliliters of the microorganism living bacteria liquid, and the content of living bacteria for the microorganism living bacteria liquid is between 1.0x10<9>cfu / mL and 1.0x10<11>cfu / mL. The fermentation waste vegetables feed and the preparation method have the advantages of reasonable formulation, scientific production method, wide applicable areas, simple operation, easy master, low cost, reasonable formula design, convenient production, suitability for broad peasant households and livestock breeding plants, safe and no toxity, and great adaptability to fermentation conditions, and can be used as feed of cows, sheep, geese, and so on, make full use of side products in agriculture industry, realize reasonable use of the resources, and provide new techniques for clean and sustainable agricultural industry.

Normal tissue complications limit curative broadbeam radiotherapy to tumors including lung cancer. Radiation retinitis causing blindness limits quality of life and long term survival for patients with ocular melanoma. This invention pertains to alternative, normal tissue sparing 100 to 1,000 Gy microbeam radiations with least normal tissue complications and concomitant radio-immunotherapy by innate immune response of epidermis and dermis to low dose radiation with 50 kV X-rays. Total body skin radiation with former airport passenger screening machines with 50 kV X-ray is disclosed. Microbeams are generated without contaminating scatter and neutron radiations from collinear gamma ray and electron beam produced by inverse Compton interaction with high energy laser and electron beam and from proton and carbon ion beams in tissue equivalent cylindrical collimators. Extracorporeal immunotherapy and chemotherapy and apheresis of mutated subcellular particles released into circulation in response to cancer-therapies are by clinical continuous flow ultracentrifugation combined chromatography.

The invention relates to the technical field of fermentation engineering, and discloses a submerged fermentation technology of producing immunological enhancement active materials of inonotus obliquus. The submerged fermentation technology of producing the immunological enhancement active materials of the inonotus obliquus comprises the main steps of culture activation, culture liquid cultivation, submerged fermentation and preparation of the immunological enhancement active materials of the inonotus obliquus and the like. The optimized submerged fermentation technology of producing the immunological enhancement active materials of the inonotus obliquus is high in content of the obtained immunological enhancement active materials, and good in activity of the obtained immunological enhancement active materials, and the obtained immunological enhancement active materials are beneficial for industrial application.

The invention provides a cell preparation for treating osteoarthritis and a preparation method thereof. The cell preparation mainly comprises mesenchymal stem cells, differentiation-induced stem cells, platelet-rich plasma and dispersion media thereof. Compared with the prior art, the cell preparation for treating osteoarthritis has the advantages that the mesenchymal stem cells are mixed with the differentiation-induced stem cells and compounded with the platelet-rich plasma and can be dispersed in the joint cavity, so that the immune regulation function of the mesenchymal stem cells can be exerted, the microenvironment of the whole joint cavity can be improved, and inflammation remission and injury repair are facilitated; tissues can be repaired after the differentiated mesenchymal stem cells are implanted into the body, so that the repair process is shortened.