21results about How to "Promote secretion" patented technology

This invention discloses an anti-inflammatory peptide derived from pearl oysters and its applications, relating to the field of bioactive peptide technology. The amino acid sequence of this anti-inflammatory peptide is TAMY. The anti-inflammatory peptide derived from pearl oysters provided by this invention can regulate the release of cellular inflammatory mediators, reduce the levels of NO and pro-inflammatory cytokines (such as TNF-α, IL-6, and IL-1β), and simultaneously promote the secretion of anti-inflammatory cytokines (such as IL-10), exhibiting excellent anti-inflammatory activity and suitable for use in functional foods, cosmetics, or pharmaceutical ingredients.

The present application relates to the field of pharmaceutical preparation and nanomedicine, and particularly relates to a kind of nano-drug composition, its combined drug delivery system and application.The nano-drug composition comprises alpha 1-AR blocker and amphiphilic nano-carrier;The alpha 1-AR blocker is loaded in the hydrophobic core formed by amphiphilic nano-carrier;The alpha 1-AR blocker is selected from one or more of prazosin, terazosin, doxazosin;The average particle size of the nano-drug composition is 220-260 nm, the polydispersity index is less than 0.3, and the encapsulation efficiency is greater than 50%.The nano-drug composition of the present application has dual functions of anti-tumor proliferation and immune microenvironment remodeling, improves the dispersion stability of the drug in physiological medium, effectively reduces the in vivo clearance rate, prolongs the circulation time, and realizes the efficient in vivo delivery of the drug.The preparation process of the nano-preparation is stable and can be produced on a large scale.

This invention discloses a guanylate cyclase-C (GC-C) activating peptide and its applications, belonging to the field of bioactive peptide technology. The amino acid sequence of the GC-C activating peptide is PFPGPIPN. The GC-C activating peptide of this invention can significantly activate GC-C activity, increasing intracellular cGMP levels. Furthermore, the GC-C activating peptide of this invention can alleviate constipation and abdominal pain symptoms in mice, thereby effectively preventing or improving irritable bowel syndrome (IBS). Therefore, the GC-C activating peptide provided by this invention has advantages such as high activity, safety and non-toxicity, and good water solubility. It can be used as a candidate molecule for alternative or adjuvant drugs in the clinical treatment of IBS, and also as a health product or food to improve constipation or abdominal pain.

This invention provides an oyster peptide and its application in enhancing immunity. The oyster peptide has a novel amino acid structure. The oyster peptide can enhance the activity of immune cells, promote the proliferation of immune cells, promote the development and maturation of immune organs, regulate the in vivo immune environment, promote the secretion of immunoglobulins, and improve humoral immunity.

The present application relates to a kind of probiotic oil agent for improving non-alcoholic fatty liver disease, preparation method and application, the probiotic oil agent includes active ingredient A and active ingredient B, the active ingredient A is mucinophilic Akkermansia Akk11, and the preservation number is CCTCC NO:M2024119;The active ingredient B is Omega-3 fatty acid.The present application is combined with mucinophilic Akkermansia Akk11 and Omega-3 fatty acid, and is prepared into oil agent, and the improvement of NAFLD symptom can play the coordinated synergistic effect, can be used for the prevention and treatment of NAFLD.

The application belongs to the field of functional substance research, and particularly relates to a corn extract for promoting GLP-1 secretion, and a preparation method and application thereof. The corn extract provided by the application is a dried product obtained by ultrasonic extraction of a dried powder of fresh corn kernels after room temperature soaking in ethanol or an ethanol aqueous solution; the fresh corn comprises yellow sweet corn; the temperature of the ultrasonic extraction is 30-60 DEG C; the power of the ultrasonic extraction is 50-150 W; and the time of the ultrasonic extraction is 30-90 min. The preparation method of the corn extract is simple and easy to implement, and can effectively reduce the production cost of functional ingredients. The corn extract can effectively promote the secretion of GLP-1 by intestinal L cells in both healthy and insulin-resistant states, and simultaneously improve cell viability.

This invention provides a biocompatible β This invention relates to a method for composite modification of titanium alloy surfaces, belonging to the technical field of alloy modification methods. The invention includes applications in medical... β This invention involves introducing bioactive particles onto the surface of a titanium alloy substrate, performing friction stir processing (FSP) on the substrate to introduce bioactive particles and construct a gradient grain structure, and then subjecting the FSP-treated substrate to compressed plasma flow (CPF) treatment to achieve uniform distribution of bioactive particles and construct a topology with an average surface roughness of 3-6 μm suitable for cell growth. The invention precisely controls the amount of bioactive particles introduced and constructs a gradient grain structure through friction stir processing (FSP); simultaneously, compressed plasma flow (CPF) technology achieves uniform distribution of bioactive particles and an ideal surface roughness of 3-6 μm on the titanium alloy surface. Ultimately, this significantly improves the cell adhesion, proliferation capacity, and osteogenic properties of the material surface, solving the technical problem of low integration efficiency of traditional titanium alloy implants with human bone tissue.

This invention belongs to the field of biotechnology, specifically relating to a high-affinity anti-tumor NK cell, its preparation method, and its application. This invention designs NK92 cells transfected with a haPD1 high-affinity chimeric conversion receptor, wherein the high-affinity chimeric conversion receptor includes the extracellular segment of haPD-1, the transmembrane segment of CD28, the intracellular segment of DAP10, and the intracellular segment of CD3ζ. This invention prepares haChR3-NK92 cells through the construction of a recombinant lentiviral vector, lentiviral packaging, and lentiviral transfection of NK92 cells. In this invention, haPD-1 serves as the extracellular recognition domain of the CAR structure, specifically binding to PD-L1 on tumor cells to achieve a stronger tumor-killing effect. The co-stimulatory molecule CD28 serves as the transmembrane region to transmit extracellular information into the cell, integrating the adaptor protein DAP10 of the NK cell surface activator receptor NKG2D into the cell, and further embedding the intracellular segment CD3ζ commonly used in CAR design to jointly promote NK cell activation. The preparation method of this invention can successfully construct haChR3-NK92 cells, achieve the expression of the target plasmid, and be applied in anti-tumor drug research.

This invention relates to the field of biopharmaceutical technology, specifically to... SARM1 The application of related reagents in the preparation of products for the diagnosis and / or treatment of hemophagocytic lymphohistiocytosis-like liver injury. The results of this invention demonstrate... SARM1 It is both a circulating biomarker in HLH and an intrinsic regulator of iNKT cell fate. SARM1 By stabilizing LCK and maintaining TCR signaling, excessive iNKT1 bias and IFN-γ-driven liver injury can be prevented. These findings highlight... SARM1 Its important value as a potential diagnostic biomarker and therapeutic target for HLH in children provides a new strategy for the treatment of hemophagocytic lymphohistiocytosis-like liver injury in children.

This utility model relates to the field of electric breast pump technology, and discloses a suction component for an electric breast pump, including a connecting shell, a duckbill valve at the lower part of the connecting shell, a suction assembly at the upper part of the connecting shell, a breast shield on one side of the connecting shell, and a vibration mechanism on the outer periphery of the breast shield. The suction component of this utility model drives a hollow cup motor, causing the hollow cup motor to rotate an eccentric block, thereby generating vibration. This vibration is transmitted to the breast shield through the fixed shell, and massage protrusions massage the breast, promoting milk secretion. A leak-proof ring is located near the opening of the breast shield to prevent leaked milk. Simultaneously, a soft sealing gasket made of medical-grade silicone is provided around the edge of the opening of the breast shield, possessing good elasticity and sealing properties, allowing it to closely conform to different breast shapes and effectively prevent milk leakage.

The application provides a spina date seed and poria cocos sleep-aiding composition, a preparation method and application thereof, and belongs to the technical field of functional food. The application uses poria cocos polysaccharide as an efficacy component and embedding carrier, and the efficacy is synergistic with spinozine and saponins in spina date seed to aid sleep, the structure is molecularly embedded spinozine and saponins in spina date seed through molecular level interaction, the bioavailability of effective components in spina date seed is significantly improved, and therefore, a better sleep-aiding effect is achieved.

This invention belongs to the field of turfgrass and forage grass maintenance technology, specifically relating to a microbial selenium protein preparation for alleviating drought stress in perennial ryegrass, its preparation method, application method, and application. The microbial selenium protein preparation comprises the following raw materials in specific weight proportions: peptone, beef extract, sucrose, salt, selenium-enriched mushroom powder, organosilicon, calcium amino acids, potassium humate, betaine, salicylic acid, and Bacillus natto. This microbial selenium protein preparation aims to solve the limitations of traditional drought-resistant methods and the poor effectiveness of single-component preparations. When using this microbial selenium protein preparation, it is first diluted and then applied as a root drench to ryegrass seedlings multiple times. The selenium protein preparation of this invention, through the synergistic effect of multiple components, can significantly improve the drought resistance of ryegrass. Furthermore, the preparation process of this protein preparation is simple, environmentally friendly, and efficient, suitable for ryegrass propagation and maintenance, and has broad application prospects.

This invention discloses a high-glutathione-producing engineered Escherichia coli strain and its applications, belonging to the field of biotechnology; the engineered Escherichia coli strain is named ZMGSH01F, with accession number CCTCC M 20252236; the engineered Escherichia coli strain is based on Escherichia coli BL21(DE3) as the starting strain, with gene clusters knocked out. ptsH‐ ptsI‐crr And integrate at this site glf‐glk Fusion genes, knockout gene clusters gsiA‐gsiB‐gsiC Integrate the endogenous glutathione transporter gene at this site. cycDC Knockout of γ-glutamyltransferase gene ggt Simultaneously expressing the recombinant plasmid pRSFDuet- gshAB K177E‑E275K‑V513A - ppk2 The engineered Escherichia coli strain can efficiently produce glutathione through fermentation culture using glucose, monosodium glutamate, cysteine, and glycine as substrates.

This invention discloses an integrated device and a method for rapid cultivation of aerobic granular sludge suitable for low-concentration domestic sewage, along with its application. The device comprises a batch reactor body integrating the following functional units: a full-area water distribution unit, including a main inlet pipe and distributed water distribution components; one end of the main inlet pipe is connected to the sewage source, and the other end extends to the bottom of the reactor cavity; the distributed water distribution components are connected to the main inlet pipe and equipped with multiple water distribution holes; a dual-zone aeration unit, including an air pump, a bottom aeration unit with a wide range of adjustable air volume, and a middle aeration unit with precise air volume control; a flow field optimization unit, located at the upper part of the reactor cavity and connected to the main inlet pipe; and a timing control unit, electrically connected to the full-area water distribution unit and the dual-zone aeration unit. The advantages of this invention are: by creating sedimentation selective pressure through dual-zone aeration to achieve sludge stratification, enhanced denitrification of heavy granular sludge, and removal of excess phosphorus from light flocculent sludge, it also has the advantages of shortening the cultivation cycle of aerobic granular sludge and highly efficient denitrification and phosphorus removal.

The present invention relates to an o / w emulsion comprising an oil phase, an aqueous phase and particles dispersed in the aqueous phase, wherein the particles are lipid nanoparticles (LNP) comprising a nucleic acid component, the particles being capable of stabilizing the oil-water interface. The invention also discloses a pharmaceutical composition, such as a vaccine, comprising the o / w emulsion.

The application discloses lactobacillus plantarum WH021 and application thereof in prevention and / or alleviation of neuroinflammation, and belongs to the technical field of microorganisms. The lactobacillus plantarum WH021 is obtained by separation and purification from healthy infant feces, is a probiotic with the effect of alleviating neuroinflammation, and has good application potential in development of food, health products and drugs for preventing brain inflammation related diseases of middle-aged and old people caused by chronic inflammation of the body.

This invention relates to the application of microalgae polysaccharides in enhancing intestinal immunity in dairy cows, belonging to the field of feed technology. The microalgae polysaccharides prepared by this invention are used to increase the secretion of IgA, IgG, and IgM by the intestinal mucosa. This invention uses a specific ratio of kelp buds, Dunaliella salina, and Haematococcus pluvialis for pre-enzymatic hydrolysis using trypsin and nattokinase for a certain period of time, followed by enzymatic hydrolysis using hemicellulase and pectinase for a certain period of time. The resulting microalgae polysaccharide product has excellent effects in promoting the secretion of immunoglobulins in the dairy cow intestine, significantly increasing the content of IgA, IgG, and IgM in the dairy cow intestine, thereby enhancing intestinal immunity. It can serve as a highly effective alternative component for improving the intestinal immune function of dairy cows.

The present application relates to the technical field of NK cell culture, and discloses a method for improving the killing function of NK cells and application thereof.The method comprises the following steps: (1) adding a haem oxygenase 1 antibody to an NK cell culture medium to prepare an antibody culture medium; (2) performing sterile filtration treatment on the antibody culture medium; and (3) separating NK cells and culturing the NK cells in the antibody culture medium.The present application adds a haem oxygenase 1 antibody to treat NK cells, blocks the expression of haem oxygenase 1 in the NK cells, or inhibits the activity of an effective substance of haem oxygenase 1.After being treated with the haem oxygenase 1 antibody, the secretion of granzyme B and perforin of the NK cells can be promoted, and the killing effect of the NK cells on target cells Yac-1 and K562 can be improved, so that the anti-tumor function of the NK cells is improved.

This invention discloses a natural plant-based liver-protecting preparation based on promoting bile secretion and its preparation, relating to the fields of natural plant medicine and health food technology. This invention effectively achieves liver protection through the synergistic effects of promoting bile secretion, choleretic detoxification, and direct protection of hepatocytes. Specifically, milk thistle extract, rich in silybin, can stabilize hepatocyte membranes, prevent toxic substances from entering hepatocytes, and promote the repair and regeneration of damaged hepatocytes; cynarin in artichoke extract accelerates the excretion of toxins and waste products by promoting bile secretion, reducing the burden on the liver; and the total flavonoids in dandelion root extract have anti-inflammatory and antioxidant effects, protecting hepatocytes from free radical damage, thereby comprehensively improving liver health.

The invention belongs to the technical field of biological medicines, and particularly relates to a GM-CSF loaded tumor cell vaccine and application thereof in prevention and treatment of liver cancer. In order to enhance the immunogenicity of the existing tumor cell vaccine, a liver cancer cell line is modified through genetic engineering, and a key cell factor GM-CSF capable of regulating and controlling differentiation, maturation, migration and functions of DCs is introduced, so that the GM-CSF is stably overexpressed, and the whole-cell tumor vaccine capable of efficiently secreting the GM-CSF is successfully constructed. And the antigen presentation and T cell starting capability of the DCs are further enhanced through ionization irradiation. Experiments prove that the GM-CSF loaded tumor cell vaccine can effectively activate specific T cell immune response, induce long-acting immune memory, inhibit immune tolerance and obviously inhibit growth of liver cancer tumors and tumor recurrence, and is high in safety. The method is hopeful to enter the development and application of clinical tumor vaccines.

This invention relates to the field of microbial fermentation technology, and particularly to *Phellinus linteus* mycelium, a method for increasing the polysaccharide yield of *Phellinus linteus* mycelium, and their applications. The method involves placing *Phellinus linteus* mycelium in an alternating magnetic field environment with a magnetic field strength of 2 mT to 20 mT and a frequency of 2 Hz to 100 Hz during the late logarithmic growth phase to the early stationary phase of its cultivation. Compared to a control group without magnetic field treatment, this method can increase the intracellular polysaccharide yield of *Phellinus linteus* mycelium by 10%–95%, while maintaining stable or slightly increased mycelial biomass. Furthermore, this method is a purely physical stimulation, introducing no chemical substances into the fermentation system, resulting in no pollution or residue, and aligns with the development direction of green biomanufacturing.