51results about How to "Promote secretion" patented technology

This invention discloses an anti-inflammatory peptide derived from pearl oysters and its applications, relating to the field of bioactive peptide technology. The amino acid sequence of this anti-inflammatory peptide is TAMY. The anti-inflammatory peptide derived from pearl oysters provided by this invention can regulate the release of cellular inflammatory mediators, reduce the levels of NO and pro-inflammatory cytokines (such as TNF-α, IL-6, and IL-1β), and simultaneously promote the secretion of anti-inflammatory cytokines (such as IL-10), exhibiting excellent anti-inflammatory activity and suitable for use in functional foods, cosmetics, or pharmaceutical ingredients.

The invention discloses a contact lens. The contact lens comprises a lens body and an electronic component. The glasses body is used for being worn on an eyeball and comprises an optical part and an annular wearing part surrounding the optical part. The annular wearing part is provided with a C-shaped lower eyelid layout area. The electronic component is arranged on the glasses body. The electronic component can move along with eyeballs through the glasses body, and the electronic component comprises a stimulation unit and a power supply unit electrically coupled with the stimulation unit. The stimulation unit comprises an electrode module arranged in the lower eyelid layout area and a boost control module electrically coupled with the electrode module. The boost control module sends out a pulse voltage signal through the electrode module, and the pulse voltage signal is used for being conducted to a lower eyelid on one side of the eyeball by body fluid.

The present application relates to a kind of nasal ala base subperiosteal implant retention method, belong to plastic surgery medical instrument technical field.The present application provides a kind of nasal ala base subperiosteal implant soft tissue suspension retention method and its implant and system.The method includes: implant is placed into maxillary bone subperiosteal interstice, and implant fixed wing is fixed with the soft tissue anchor point such as alar base ligament of alar elevating lip muscle using drug-loaded degradable suture and is suspended and fixed.Implant surface is provided with the microporous structure of guiding tissue ingrowth, and is provided with the drug depot recess of filling load immunomodulator temperature-sensitive hydrogel.Postoperative healing process, suture is released under the action of tension to accelerate proangiogenic factor and promote local vascularization, and temperature-sensitive hydrogel slow-release immunomodulator induces macrophage to repair type polarization, and cooperatively promotes the biological chimeric fixation of implant and soft tissue.The present application can realize the double stable retention of implant, reduces postoperative displacement risk.

The invention discloses a composition with an anti-aging effect and a preparation method and application thereof, and belongs to the technical field of cosmetics. The composition is prepared by compounding a traditional Chinese medicine active component, a moisturizing and permeation promoting component and a repairing and anti-aging auxiliary component, and the traditional Chinese medicine active component is composed of three zymolytes and three fermentation extracts which are respectively prepared from six medicinal and edible traditional Chinese medicine raw materials through differentiated composite enzymolysis and probiotic anaerobic fermentation processes. The problems that traditional Chinese medicine extracts are low in transdermal absorption rate, poor in bioavailability and insufficient in cosmetic system stability are solved, active ingredients are small-molecularized through process optimization, a multi-target synergistic anti-aging system is formed by matching with functional components, the anti-aging and repairing composition has excellent anti-aging and repairing effects, physical and chemical stability and skin safety, and the anti-aging and repairing composition has a good application prospect. The composition is suitable for various skin types and can be widely applied to preparation of anti-aging repair cosmetics.

The present application relates to the field of pharmaceutical preparation and nanomedicine, and particularly relates to a kind of nano-drug composition, its combined drug delivery system and application.The nano-drug composition comprises alpha 1-AR blocker and amphiphilic nano-carrier;The alpha 1-AR blocker is loaded in the hydrophobic core formed by amphiphilic nano-carrier;The alpha 1-AR blocker is selected from one or more of prazosin, terazosin, doxazosin;The average particle size of the nano-drug composition is 220-260 nm, the polydispersity index is less than 0.3, and the encapsulation efficiency is greater than 50%.The nano-drug composition of the present application has dual functions of anti-tumor proliferation and immune microenvironment remodeling, improves the dispersion stability of the drug in physiological medium, effectively reduces the in vivo clearance rate, prolongs the circulation time, and realizes the efficient in vivo delivery of the drug.The preparation process of the nano-preparation is stable and can be produced on a large scale.

This invention discloses a guanylate cyclase-C (GC-C) activating peptide and its applications, belonging to the field of bioactive peptide technology. The amino acid sequence of the GC-C activating peptide is PFPGPIPN. The GC-C activating peptide of this invention can significantly activate GC-C activity, increasing intracellular cGMP levels. Furthermore, the GC-C activating peptide of this invention can alleviate constipation and abdominal pain symptoms in mice, thereby effectively preventing or improving irritable bowel syndrome (IBS). Therefore, the GC-C activating peptide provided by this invention has advantages such as high activity, safety and non-toxicity, and good water solubility. It can be used as a candidate molecule for alternative or adjuvant drugs in the clinical treatment of IBS, and also as a health product or food to improve constipation or abdominal pain.

This invention provides an oyster peptide and its application in enhancing immunity. The oyster peptide has a novel amino acid structure. The oyster peptide can enhance the activity of immune cells, promote the proliferation of immune cells, promote the development and maturation of immune organs, regulate the in vivo immune environment, promote the secretion of immunoglobulins, and improve humoral immunity.

This invention provides a fiber-based biomimetic polyurethane artificial blood vessel and its preparation method. The method involves using wet spinning technology to obtain filaments, which are then drawn and wound onto the surface of a metal tube to form a polyurethane fiber inner layer. A fabric middle layer is woven onto the surface of the polyurethane fiber inner layer. An organic solvent is sprayed onto the surface of the fabric middle layer, and an outer polyurethane fiber layer is prepared on the fabric surface using electrospinning technology. Finally, after curing and demolding, the fiber-based biomimetic polyurethane artificial blood vessel is obtained. This invention combines wet spinning, weaving, and electrospinning technologies to achieve integrated preparation of polyurethane artificial blood vessels. The resulting artificial blood vessel is of high quality, with a tightly bonded three-layer fiber-based structure that maximally mimics the three-layer structure of natural blood vessels. The inner layer provides excellent compliance, the fabric layer increases the blood vessel's impermeability and sutureability, and the outer layer effectively prevents vascular delamination and promotes endothelialization. Furthermore, the preparation method is environmentally friendly and pollution-free, with a short process flow, making it suitable for continuous industrial production.

This invention discloses a strain of *Lactobacillus salivarius* CCFM1332 with multi-target regulatory effects and its application in periodontitis, belonging to the field of microbial technology. This invention yields a strain of *Lactobacillus salivarius* CCFM1332, which not only has vaginal immunomodulatory effects but also alleviates periodontitis. Specifically, it can: promote the secretion of antimicrobial peptides by vaginal epithelial cells VK2 / E6E7; reduce the periodontal probing depth in rats with periodontitis; reduce the number of pathogenic bacteria in the oral cavity of rats with periodontitis; downregulate the levels of serum inflammatory factors IL-1β, IL-6, and IL-17A; and reduce alveolar bone loss in rats with periodontitis, effectively alleviating alveolar bone resorption in rats.

The present application relates to a kind of probiotic oil agent for improving non-alcoholic fatty liver disease, preparation method and application, the probiotic oil agent includes active ingredient A and active ingredient B, the active ingredient A is mucinophilic Akkermansia Akk11, and the preservation number is CCTCC NO:M2024119;The active ingredient B is Omega-3 fatty acid.The present application is combined with mucinophilic Akkermansia Akk11 and Omega-3 fatty acid, and is prepared into oil agent, and the improvement of NAFLD symptom can play the coordinated synergistic effect, can be used for the prevention and treatment of NAFLD.

The application belongs to the field of functional substance research, and particularly relates to a corn extract for promoting GLP-1 secretion, and a preparation method and application thereof. The corn extract provided by the application is a dried product obtained by ultrasonic extraction of a dried powder of fresh corn kernels after room temperature soaking in ethanol or an ethanol aqueous solution; the fresh corn comprises yellow sweet corn; the temperature of the ultrasonic extraction is 30-60 DEG C; the power of the ultrasonic extraction is 50-150 W; and the time of the ultrasonic extraction is 30-90 min. The preparation method of the corn extract is simple and easy to implement, and can effectively reduce the production cost of functional ingredients. The corn extract can effectively promote the secretion of GLP-1 by intestinal L cells in both healthy and insulin-resistant states, and simultaneously improve cell viability.

This invention provides a biocompatible β This invention relates to a method for composite modification of titanium alloy surfaces, belonging to the technical field of alloy modification methods. The invention includes applications in medical... β This invention involves introducing bioactive particles onto the surface of a titanium alloy substrate, performing friction stir processing (FSP) on the substrate to introduce bioactive particles and construct a gradient grain structure, and then subjecting the FSP-treated substrate to compressed plasma flow (CPF) treatment to achieve uniform distribution of bioactive particles and construct a topology with an average surface roughness of 3-6 μm suitable for cell growth. The invention precisely controls the amount of bioactive particles introduced and constructs a gradient grain structure through friction stir processing (FSP); simultaneously, compressed plasma flow (CPF) technology achieves uniform distribution of bioactive particles and an ideal surface roughness of 3-6 μm on the titanium alloy surface. Ultimately, this significantly improves the cell adhesion, proliferation capacity, and osteogenic properties of the material surface, solving the technical problem of low integration efficiency of traditional titanium alloy implants with human bone tissue.

This invention belongs to the field of biotechnology, specifically relating to a high-affinity anti-tumor NK cell, its preparation method, and its application. This invention designs NK92 cells transfected with a haPD1 high-affinity chimeric conversion receptor, wherein the high-affinity chimeric conversion receptor includes the extracellular segment of haPD-1, the transmembrane segment of CD28, the intracellular segment of DAP10, and the intracellular segment of CD3ζ. This invention prepares haChR3-NK92 cells through the construction of a recombinant lentiviral vector, lentiviral packaging, and lentiviral transfection of NK92 cells. In this invention, haPD-1 serves as the extracellular recognition domain of the CAR structure, specifically binding to PD-L1 on tumor cells to achieve a stronger tumor-killing effect. The co-stimulatory molecule CD28 serves as the transmembrane region to transmit extracellular information into the cell, integrating the adaptor protein DAP10 of the NK cell surface activator receptor NKG2D into the cell, and further embedding the intracellular segment CD3ζ commonly used in CAR design to jointly promote NK cell activation. The preparation method of this invention can successfully construct haChR3-NK92 cells, achieve the expression of the target plasmid, and be applied in anti-tumor drug research.

This invention relates to the field of biopharmaceutical technology, specifically to... SARM1 The application of related reagents in the preparation of products for the diagnosis and / or treatment of hemophagocytic lymphohistiocytosis-like liver injury. The results of this invention demonstrate... SARM1 It is both a circulating biomarker in HLH and an intrinsic regulator of iNKT cell fate. SARM1 By stabilizing LCK and maintaining TCR signaling, excessive iNKT1 bias and IFN-γ-driven liver injury can be prevented. These findings highlight... SARM1 Its important value as a potential diagnostic biomarker and therapeutic target for HLH in children provides a new strategy for the treatment of hemophagocytic lymphohistiocytosis-like liver injury in children.

The invention provides an active peptide and application thereof in improving immunity. The active peptide has a brand-new structure. The bioactive peptide can promote proliferation of immune cells, and can assist the immune cells in resisting oxidative damage, inhibiting MDA expression and improving the SOD expression level; the active peptide can improve the anti-fatigue ability of a body, promote secretion of immune globulin and strengthen the immune function of the body.

This utility model relates to the field of electric breast pump technology, and discloses a suction component for an electric breast pump, including a connecting shell, a duckbill valve at the lower part of the connecting shell, a suction assembly at the upper part of the connecting shell, a breast shield on one side of the connecting shell, and a vibration mechanism on the outer periphery of the breast shield. The suction component of this utility model drives a hollow cup motor, causing the hollow cup motor to rotate an eccentric block, thereby generating vibration. This vibration is transmitted to the breast shield through the fixed shell, and massage protrusions massage the breast, promoting milk secretion. A leak-proof ring is located near the opening of the breast shield to prevent leaked milk. Simultaneously, a soft sealing gasket made of medical-grade silicone is provided around the edge of the opening of the breast shield, possessing good elasticity and sealing properties, allowing it to closely conform to different breast shapes and effectively prevent milk leakage.

The invention provides a quaternary high-entropy alloy nano enzyme as well as a preparation method and application thereof, and belongs to the technical field of nano biological materials and biological medicines. The quaternary high-entropy alloy nano-enzyme comprises a single-phase alloy and polyvinylpyrrolidone, the single-phase alloy is composed of four metal elements of M, rhodium, iridium and ruthenium, the surface of the single-phase alloy is modified with the polyvinylpyrrolidone, the element M is selected from palladium or platinum, and the particle size of the quaternary high-entropy alloy nano-enzyme is smaller than 10 nm. The quaternary high-entropy alloy nano-enzyme disclosed by the invention can penetrate through a blood brain barrier, is enriched at a cerebral hemorrhage focus, and comprehensively blocks reactive oxygen species (ROS) cascade reaction after cerebral hemorrhage.

The invention relates to the technical field of microorganisms, and particularly discloses lactobacillus delbrueckii subsp. Bulgaricus KFSY10 and application thereof in the aspect of improving the immune function, the lactobacillus delbrueckii subsp. Bulgaricus KFSY10 is preserved in the China General Microbiological Culture Collection Center on May 2, 2018, the preservation address is CGMCC (China General Microbiological Culture Collection Center), and the preservation number is CGMCC NO. The Lactobacillus delbrueckii subsp. Bulgaricus is located in Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing, has a preservation number of CGMCCNO: 15717, and is classified and named as Lactobacillus delbrueckii subsp. Bulgaricus KFSY10. The lactobacillus delbrueckii subsp. Bulgaricus KSFY10 provided by the invention can be used for directly improving the immune function of an immunosuppressive mouse by protecting and maintaining normal physiological structures of central and peripheral immune organs and promoting secretion of serum cytokines and immune globulins; and the immunologic function of the mouse can be indirectly promoted by regulating and controlling acute and chronic inflammatory reactions of intestinal tracts, so that side effects and adverse reactions caused by the use of chemical drugs are effectively avoided.

The invention discloses application of a herba houttuyniae polysaccharide extract in a feed capable of improving intestinal health of piglets, the feed comprises the herba houttuyniae polysaccharide extract and a basic feed, and the application method of the herba houttuyniae polysaccharide extract comprises the following steps: adding the herba houttuyniae polysaccharide extract into the basic feed, and continuously feeding 21-28-day-old weaned piglets for 28 days, the intestinal flora structure of weaned piglets is adjusted through the houttuynia cordata polysaccharide extract, short-chain fatty acid secretion is promoted, and the intestinal morphology is improved, so that diarrhea prevention and intestinal health improvement are achieved; the herba houttuyniae polysaccharide extract is prepared from the following components in percentage by mass: 20 percent to 21.6 percent of herba houttuyniae polysaccharide, 5 percent to 10 percent of volatile oil, 25 percent to 35 percent of flavonoid, 10 percent to 15 percent of total amino acid, 3.1 percent to 6.1 percent of alkaloid, 3 percent to 10 percent of organic acid and 1.5 percent to 5.5 percent of sterol, and the herba houttuyniae polysaccharide is prepared from glucose, galactose, mannose, arabinose and xylose. The invention aims to improve the survival rate of early weaned piglets, shorten the breeding cycle of a farm and improve the reproductive performance.

This invention discloses a heat-stable bovine alpha-interferon mutant, the amino acid sequence of which is shown in SEQ ID NO.7. The mutant introduces cysteine ​​mutations at amino acid positions 55 and 112, forming an S55C+L112C disulfide bond, compared to wild-type bovine alpha-interferon; the amino acid sequence of wild-type bovine alpha-interferon is shown in SEQ ID NO.1. This invention is the first to propose a disulfide bond modification scheme for bovine alpha-interferon that balances heat stability and antiviral activity, adapted to the Pichia pastoris high-efficiency secretory expression system. The modified mutant exhibits 14.7-fold increased antiviral activity compared to the wild type. At 56°C for 1 hour, the mutant shows significantly improved heat resistance, with an antiviral activity reaching 1.0 × 10⁻⁶. 7.0 IU / ml. The activity is stable after long-term storage at room temperature or 37°C. After storage at room temperature for six months and at 37°C for two months, the antiviral activity still retains 1.0 × 10⁻⁶. 6.0 IU / ml, thus solving the problems of poor stability, easy inactivation under high temperature conditions, and difficulty in transportation at room temperature of wild-type bovine alpha-interferon.

The application discloses a traditional Chinese medicine probiotic agent with spleen and stomach function regulation and a preparation method thereof. The traditional Chinese medicine probiotic agent is composed of A component oligosaccharide and B component traditional Chinese medicine extract. The probiotic agent is a mixture of microorganisms, such as fermented lactic acid bacteria, plant lactobacillus, animal bifidobacterium, rhamnose lactic acid bacteria, swiss lactic acid bacteria and acidophilic lactobacillus. The traditional Chinese medicine food materials are composed of cassia, red mushroom, golden camellia, pueraria, dried tangerine or orange peel, lemon, vitis lamprochloa, moringa leaf, fig, eucommia ulmoides, cistanche and momordica grosvenorii. The traditional Chinese medicine food material extract and the probiotic agent are synergistically used, the problems of single function, low bioavailability and unsuitability for the internal environment of the human intestinal stomach with cereal as staple food of existing products are effectively solved, the functions of stomach, intestine, spleen, liver, gallbladder and kidney are improved, and a safer and more efficient, more targeted health solution is provided for the people with digestive system function disorder, especially spleen and stomach function disorder.

The application provides a spina date seed and poria cocos sleep-aiding composition, a preparation method and application thereof, and belongs to the technical field of functional food. The application uses poria cocos polysaccharide as an efficacy component and embedding carrier, and the efficacy is synergistic with spinozine and saponins in spina date seed to aid sleep, the structure is molecularly embedded spinozine and saponins in spina date seed through molecular level interaction, the bioavailability of effective components in spina date seed is significantly improved, and therefore, a better sleep-aiding effect is achieved.

The present application provides a Chinese herbal medicine composition and its application in preparing a medicine for treating chronic functional constipation. The Chinese herbal medicine composition comprises the following components in mass parts: 2.3-2.7 parts of radix rehmanniae, 1.2-1.5 parts of angelica sinensis, 1.5-1.7 parts of persicae semen, 1-1.4 parts of radix bupleuri, 0.8-1 part of citri reticulatae pericarpium, 0.3-0.4 part of aurantii fructus, and 0.3-0.6 part of glycyrrhizae radix. The Chinese herbal medicine composition can effectively improve defecation, improve the peristalsis of the intestinal tract, and repair the morphology of the colon, and is very suitable for treating chronic functional constipation.

The invention discloses a bacillus licheniformis recombinant strain with high yield of L-cysteine, and belongs to the technical field of synthetic biology and microbial metabolism engineering. The cysteine synthesis flux is enhanced through multiple knockout and combined overexpression. An efficient engineering strain is constructed by knocking out a serine hydroxymethyltransferase coding gene glyA and an L-serine ammonolysis enzyme coding gene sdaAA of a serine catabolism key pathway, knocking out a cysteine catabolism gene metC and combining gene overexpression to introduce a feedback inhibition removal mutant cysEf and a transporter eamA, and the efficient engineering strain is used for preparing a recombinant strain. Finally, the yield of the L-cysteine reaches 1.075 g / L in shake flask fermentation (the conversion rate is 18.69%), and the application potential of the bacillus licheniformis in sulfur metabolism engineering is highlighted.

This invention belongs to the field of turfgrass and forage grass maintenance technology, specifically relating to a microbial selenium protein preparation for alleviating drought stress in perennial ryegrass, its preparation method, application method, and application. The microbial selenium protein preparation comprises the following raw materials in specific weight proportions: peptone, beef extract, sucrose, salt, selenium-enriched mushroom powder, organosilicon, calcium amino acids, potassium humate, betaine, salicylic acid, and Bacillus natto. This microbial selenium protein preparation aims to solve the limitations of traditional drought-resistant methods and the poor effectiveness of single-component preparations. When using this microbial selenium protein preparation, it is first diluted and then applied as a root drench to ryegrass seedlings multiple times. The selenium protein preparation of this invention, through the synergistic effect of multiple components, can significantly improve the drought resistance of ryegrass. Furthermore, the preparation process of this protein preparation is simple, environmentally friendly, and efficient, suitable for ryegrass propagation and maintenance, and has broad application prospects.

The application belongs to the technical field of aquaculture, and particularly relates to a water temperature-hormone synergistically regulated aquaculture population breeding and hatching method and application. The growth regulator is butyric acid or sodium butyrate. The growth regulator is used to enhance the breeding promotion effect of luteinizing hormone-releasing hormone or its analogs. The breeding promotion refers to promoting the growth of oocytes, promoting yolk accumulation, shortening the ovarian development cycle, and improving the egg production rate. It is found and verified for the first time that butyric acid and its sodium salt can enhance the breeding promotion effect of luteinizing hormone-releasing hormone.

The invention discloses a sodium alginate-beta-glucan composite wound repair spray with rapid film forming and long-acting repair functions and a preparation method thereof. According to the preparation method, sodium alginate is subjected to low oxidation degree modification, so that the sodium alginate and hydroxypropyl methylcellulose with viscosity gradient regulated and controlled form a high-ion response composite system, the obtained spray can quickly form a film after being sprayed, and meanwhile, yeast beta-glucan is subjected to phosphorylation modification, so that the water solubility and the targeting property are improved; according to the present invention, the binding force with the macrophage receptor is enhanced, the anti-inflammatory efficiency is improved, and the ultrasonic-microwave synergistic swelling, gradient dropwise adding-dynamic shearing cross-linking and other methods are adopted during the preparation, such that the uniformity, the stability and the safety of the product are improved. The obtained spray has multiple effects of rapid film formation, mild bacteriostasis and long-acting repair, can break through vicious circle of chronic wound repair, and provides an innovative thought for development of wound nursing products.

This invention discloses a high-glutathione-producing engineered Escherichia coli strain and its applications, belonging to the field of biotechnology; the engineered Escherichia coli strain is named ZMGSH01F, with accession number CCTCC M 20252236; the engineered Escherichia coli strain is based on Escherichia coli BL21(DE3) as the starting strain, with gene clusters knocked out. ptsH‐ ptsI‐crr And integrate at this site glf‐glk Fusion genes, knockout gene clusters gsiA‐gsiB‐gsiC Integrate the endogenous glutathione transporter gene at this site. cycDC Knockout of γ-glutamyltransferase gene ggt Simultaneously expressing the recombinant plasmid pRSFDuet- gshAB K177E‑E275K‑V513A - ppk2 The engineered Escherichia coli strain can efficiently produce glutathione through fermentation culture using glucose, monosodium glutamate, cysteine, and glycine as substrates.

The invention provides an extraction method and application of Witzia ciliaris with the preservation number of CGMCC No.33498 and extracellular vesicles of the Witzia ciliaris. The Witzia ciliaris is taken as a core, and an improved MRS culture medium and a staged culture mode are combined, so that the Witzia ciliaris can be pertinently promoted to secrete EVs, and the extracellular vesicles of the Witzia ciliaris can be obtained. The technical problems that the existing EVs is low in yield, needs to depend on special instruments or high-risk technologies and is high in cost are solved, and a safe and efficient new scheme is provided for non-drug intervention of sleep dysfunction.

This invention discloses an integrated device and a method for rapid cultivation of aerobic granular sludge suitable for low-concentration domestic sewage, along with its application. The device comprises a batch reactor body integrating the following functional units: a full-area water distribution unit, including a main inlet pipe and distributed water distribution components; one end of the main inlet pipe is connected to the sewage source, and the other end extends to the bottom of the reactor cavity; the distributed water distribution components are connected to the main inlet pipe and equipped with multiple water distribution holes; a dual-zone aeration unit, including an air pump, a bottom aeration unit with a wide range of adjustable air volume, and a middle aeration unit with precise air volume control; a flow field optimization unit, located at the upper part of the reactor cavity and connected to the main inlet pipe; and a timing control unit, electrically connected to the full-area water distribution unit and the dual-zone aeration unit. The advantages of this invention are: by creating sedimentation selective pressure through dual-zone aeration to achieve sludge stratification, enhanced denitrification of heavy granular sludge, and removal of excess phosphorus from light flocculent sludge, it also has the advantages of shortening the cultivation cycle of aerobic granular sludge and highly efficient denitrification and phosphorus removal.