242 results about "Helicase" patented technology

This invention discloses immunostimulatory combinations of Tumor Necrosis Factor Receptor Superfamily (TN-FRSF) agonists, Toll-Like Receptor (TLR) agonists, “domain present in NAIP, CIITA, HET-E, TP-I (NACHT)-Leucine Rich Repeat (LRR)” or “NLR” agonists, RIG-I-Like Helicase or “RLH” agonists, purinergic receptor agonists and cytokine / chemokine receptor agonists, together with delivery methods. The combinations, when used alone at the site of pathology, provide immunostimulation that induces host humoral and cellular immunologic responses to eliminate pathogens or neoplasms. Alternatively, when the combinations are used with a defined antigens, these combinations can induce focused humoral and cellular immunologic responses useful as prophylactic and / or ameliorative therapeutic modalities for infections and the treatment of neoplastic disorders.

Methods and a kit are provided for selectively and exponentially amplifying nucleic acids and include the use of a single strand helicase preparation or a thermostable helicase in the absence of a single strand binding protein and a DNA polymerase such that the amplification can be performed isothermally.

A helicase-mediated amplification method for circular DNA templates and target DNA sequences within the templates is provided. The method combines a DNA polymerase and a helicase preparation to amplify a target sequence as well as the entire circular DNA template.

Methods and compositions are provided for amplifying RNA from an RNA template. Methods and compositions are further provided for detecting an RNA in an RNA containing sample. Compositions used for the amplification of RNA from RNA include a RNA-dependent RNA-polymerase, a RNA helicase, and an energy source. Illustrative RNA-dependent RNA-polymerase enzymes are derived from the tomato or tobacco plant, while illustrative RNA helicase enzymes include the eIF4A and eIF4B proteins.

The invention relates to a new method of characterising a target polynucleotide. The method uses a pore and a Dda helicase. The helicase controls the movement of the target polynucleotide through the pore. The invention also relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.

The invention relates to a new method of characterising a target polynucleotide. The method uses a pore and an XPD helicase. The helicase controls the movement of the target polynucleotide through the pore.

The invention relates to a new method of characterising a target polynucleotide. The method uses a pore and a Hel308 helicase or amolecular motor which is capable of binding to the target polynucleotide at an internal nucleotide. The helicase or molecular motor controls the movement of the target polynucleotide through the pore.

The invention relates to a new method of characterising a target polynucleotide. The method uses a pore and a RecD helicase. The helicase controls the movement of the target polynucleotide through the pore.

Methods and a kit are provided for selectively and exponentially amplifying nucleic acids and include the use of a helicase preparation and a DNA polymerase such that the amplification can be performed isothermally.

The invention relates to a new method of characterizing a target polynucleotide. The method uses a pore and an XPD helicase. The helicase controls the movement of the target polynucleotide through the pore.

The invention relates to the X-ray crystal structure of the hepatitis C virus helicase domain. More specifically, the invention relates to crystallized complexes of HCV helicase and an oligonucleotide, to crystallizable compositions of HCV helicase and an oligonucleotide and to methods of crystallizing an HCV helicase-oligonucleotide complex. The invention further relates to a computer programmed with the structure coordinates of the HCV helicase oligonucleotide binding pocket or the HCV helicase nucleotide triphosphate pocket wherein said computer is capable of displaying a three-dimensional representation of that binding pocket.

The invention discloses an isothermal nucleic acid amplification reaction reagent which comprises Tris buffer solutions, potassium acetate, magnesium acetate, dithiothreitol, polyethylene glycol, adenosine triphosphate (ATP), deoxy-ribonucleoside triphosphate (dNTPs), phosphocreatine, creatine kinase, a primer group, single-strand binding (SSD) protein, colon bacillus helicase RecQ protein, UvsY protein and DNA polymerase. The invention further discloses an isothermal nucleic acid amplification method which includes steps of extracting DNA or performing inverse transcription after DNA extracting, and adding the isothermal nucleic acid amplification reaction reagent and reacting the mixture at a temperature in a range between 25 DEG C and 45 DEG C for 10 minutes to 60 minutes to complete the nucleic acid amplification. Compared with traditional polymerase chain reaction (PCR) technologies and according to the technology, nearly no professional instrument is needed, the operation is simple, technical requirements for operators are low, and the reaction time only needs about half an hour.

The invention discloses a method for extraction and preservation of helicase. According to the method, a snail is selected and treated, and the digestive juice of the snail is extracted, filtered and then centrifugalized for refinement, and then subjected to a vacuum freeze-drying treatment so as to be packed and stored, with 9 kinds of enzyme valuable in application effectively extracted from the snail digestive gland. Adoption of the method provided in the invention can realize the volume production of helicase which can be preserved for 10 years at a normal temperature, while helicase products still maintain good activity. Therefore, the method has good economic benefit.

The invention relates to a primer pairs for rapid detection of toxoplasmosis nucleic acid, a kit and a detection method. The primer pairs for rapid detection of toxoplasmosis nucleic acid comprises aforward primer, a reverse primer and a probe for detecting genome B1 gene sequence of toxoplasmosis. The kit comprises the following contents: a lysate, a diluent, a lyophozyme tube containing the primers, a positive quality control and a negative quality control. The detection includes the process of carrying out a normal-temperature nucleic acid amplification technology by using the kit. Throughmultiple enzymatic reactions of DNA helicase, single-stranded DNA binding protein, DNA polymerase and the like, target DNA can be amplified by millions of times under the condition of constant temperature 37-45 DEG C within 10-30 min; and with the cooperation of the fluorescence detection technique, rapid detection of DNA to be tested can be realized. The detection method of the invention has advantages of simple operation, short time and low requirement on equipment, and is very suitable for rapid diagnosis of pet infectious diseases.

The invention relates to methods using constructs comprising a helicase and an additional polynucleotide binding moiety. The helicase is attached to the polynucleotide binding moiety and the construct has the ability to control the movement of a polynucleotide. The constructs can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.

The invention provides a biological sporoderm-breaking technology and an effective antioxidation method for ganoderma lucidum spore powder. Firstly, full mature ganoderma lucidum spores are screened and put into water to be cleaned, then the ganoderma lucidum spores are heated after being frozen, spore enzymolysis and sporoderm breaking are carried out with one of expansin, cellulose, lysozyme, helicase and the like or the mixture of a plurality of enzymes for 3-24 hours at the temperature of 37-45 DEG C, pH (Potential Of Hydrogen) is regulated to 5.5-6.8 by citric acid, and then a vitamin C is used as an antioxidant of the ganoderma lucidum spore powder. Finally, 1% of sweetener-stevioside is used for removing bitter, and treatment with an instantaneous ultra-high temperature sterilization machine and bagging are carried out. The method disclosed by the invention has a good sterilization effect and high sporoderm breaking efficiency, is low in cost, does not break protoplasm of the ganoderma lucidum spore powder and keeps the original characteristics of the ganoderma lucidum powder.

Methods for rapidly detecting clinically relevant mutations in the infectious genome of an agent are disclosed. The methods include use of a novel target and temperature dependent RNase H mediated cleavage of blocked DNA primers to initiate isothermal helicase-dependent amplification of a target sequence such as a sequence in the rpoB gene.

Disclosed herein are methods of amplifying a target nucleic acid in a helicase-dependent reaction. Also disclosed are methods of amplifying and detecting a target nucleic acid in a helicase-dependent reaction as well as modified detection labels to assist in the detection.

The invention relates to a polycyclic compound for inhibiting RNA helicase DHX33. In particular, the invention relates to a compound shown as a formula I or a pharmaceutically acceptable form thereof, a pharmaceutical composition containing the compound, a preparation method of the compound, and medical application of the compound to prevention and / or treatment of DHX33 related diseases.

The invention belongs to protein detection in the biomedical field, and in particular provides a DNA helicase detection method based on a graphene oxide chip. The DNA helicase detection method based on the graphene oxide chip comprises the following steps: (1), preparing a slide with graphene oxide dot matrixes; (2), connecting a double-chain DNA sequence with the slide with the graphene oxide dot matrixes; (3) detecting DNA helicase, to be specific, adding different quantities of DNA helicases at different action time, performing PBS washing to scan and analyze, and detecting according to FAM fluorescence intensity change. According to the DNA helicase detection method based on the graphene oxide chip, fluorescene on FAM marked by a single-stranded DNA can be quenched by utilizing a graphene oxide; after the DNA helicase is applied to a double-stranded DNA, a single-stranded DNA separates from the complementary single-stranded DNA fixed on the graphene oxide, and the distance between the FAM and the surface of the graphene oxide is changed, so that the change of FAM fluorescence intensity is caused; by combining a high-throughput microarray chip technique, the DNA helicase can be detected with low cost and high sensitivity.

The invention relates to an extraction method of a mycelial fungus genome. Enzymolysis is performed on Penicillium chrysogenum, Aspergillus niger, Trichoderma reesei, penicillium citrinum and other mycelial fungus hyphae by using a helicase-cellulase mixed enzyme solution, and the enzymolysis treatment is effectively combined with ultrasonic treatment and high-temperature heating treatment, thereby obtaining the mycelial-fungus-genome-containing supernate for PCR (polymerase chain reaction) identification. The supernate of the mycelial fungus hypha enzymolysis solution obtained by the extraction method is directly used for PCR reaction to obtain the mycelial fungus 18S rDNA gene strip without purification operation. The operational process is simple, safe and stable, avoids the use of the toxic extraction reagent, and reduces the injury to the experimental personnel.

The present invention relates to a method for amplifying a template nucleic acid, wherein the method comprises amplifying said template nucleic acid using the helicase dependent amplification (HDA) reaction in the presence of a nicking endonuclease, and wherein said template nucleic acid comprises a sequence recognized by said nicking endonuclease or a sequence recognized by said nicking endonuclease is introduced into the template nucleic acid during the HDA reaction. The invention further pertains to a kit for amplifying a nucleic acid, comprising a nicking endonuclease, a helicase and a DNA polymerase.

The invention relates to a helicase which is a compound enzyme extracted from a snail crop or a snail digestive tract, the helicase mainly contains twenty kinds of enzyme including cellulose, pectinase, amylase, prolease and the like and is a byproduct in comprehensive utilization and development of snails, however, in the practical processing, the internal organs of the snail are always discarded, so that great waste of enzyme resources is caused. The helicase is a good compound enzyme system containing the pectinase, the amylase and the cellulose; the helicase which is a natural compound enzyme system is applied to a biological refining process of a cotton woven fabric to remove pectin, waxiness and other impurities in cotton fiber under the hydrolytic action of the helicase, therefore, the purposes of improving the wettability and the whiteness of the cotton fabric are achieved. The helicase is adopted for refining the cotton fabric, the helicase has the environment-protecting and energy-saving characteristics, the snail processing byproducts can be comprehensively utilized, the requirement for the current sustainable development is met, and the comprehensive development of the dyeing and finishing industry and the processing industry for snail agriculture products are facilitated.

The invention relates to the technical field of microbial enhanced oil recovery, and in particular relates to an extraction method of oil reservoir microbial genome DNA. The method comprises the following steps: removing interference of crude oil in samples with extraction by means of organic solvent extraction; releasing microbes on an oil-water interface; filtering water phase by using a hollow fiber membrane to enrich microbes; removing organic macromolecular components such as proteins by phenol and chloroform in cooperation with lysis cells such as lysozyme, helicase, protease and lauryl sodium sulfate according to a physical wall-breaking method under a high-salinity condition; subsequently, performing nucleic acid extraction and precipitation to obtain the oil reservoir microbial genome DNA. The method is quick and simple, and the whole extraction process can be completed in 10 hours; the extraction efficiency is high, and the purity of the obtained genome is high.