The invention discloses an environment-friendly alcohol-free fountain solution for offset printing and a preparing method thereof. The solution is prepared from, by weight, 4-7 parts of lauryl polyoxyethylene, 6-8 parts of coconut diethanolamide, 3-5 parts of petroleum sodium sulfonate, 4-6 parts of potassium pyrophosphate, 10-14 parts of trisodium citrate, 8-10 parts of Arabic gum, 4-5 parts of dodecyl polyglucoside, 2-4 parts of hydroxyethyl ethylenediamine, 7 parts of dimethyl dodecyl amine oxide, 2-3 parts of di-o-tolyl-thiourea, 1-2 parts of sodium carboxymethylcellulose, 0.6-0.8 part of methylisothiazolinone, 4-5 parts of Turkey red oil, 0.8-1.2 parts of ethylene diamine tetraacetic acid tetrasodium, 0.6-0.8 part of tea saponin, 3-5 parts of dipropylene glycol methyl ether, 3-4 parts of dodecyl betaine type phosphate, 2-3 parts of sodium hexametaphosphate, and 120-140 parts of deionized water. According to the fountain solution, alcohol of any kinds is not added, no volatile harmful substance is contained, environment friendliness is realized completely, no harm can be done to the human body, a quite strong microorganism killing effect can be realized, microbial pollution of the fountain solution can be controlled and prevented, metal parts can be prevented from being corroded, and a printing plate and a printing machine are prevented from being damaged by early water.