Method for testing and evaluating capacity of corrosion prevention system of cosmetic
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A capability testing and cosmetic technology, applied in the field of capability testing and evaluation of cosmetic anti-corrosion systems, can solve the problems of prolonging production cycle, increasing production cost, complicated operation, etc., and achieving the effect of accurate judgment, strong reliability and wide adaptability
Inactive Publication Date: 2009-10-07
TIANJIN TIANSHI BIOLOGICAL DEV +2
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However, the evaluation of the antiseptic ability of existing cosmetics requires frequent microbial inspections,
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preparation example Construction
[0035] Preparation of preparation media and reagents:
[0036] 1. Physiological saline:
[0037] Ingredients: Sodium Chloride 8.5g;
[0038] Add distilled water to 1000ml; after dissolving, dispense into Erlenmeyer flasks with glass beads, 90ml per bottle, and autoclave at 121°C (15lb) for 20min.
[0039] 2. Lecithin-Tween 80-nutrient agar medium;
[0040] 2.1. Ingredients: 20g of protein;
[0041] Beef extract 3g;
[0042] Sodium chloride 5g;
[0043] Agar 15g;
[0044] Lecithin 1g;
[0045] Tween 80 7g;
[0046] Distilled water 1000ml.
[0047] 2.2. Preparation method: first add lecithin to a small amount of distilled water, heat to dissolve, add Tween-80, add other ingredients (except agar) to the rest of distilled water, dissolve, add dissolved lecithin, Tween 80, mix well, adjust the pH to 7.1-7.4, add agar, autoclave at 121°C (15lb) for 20min, and store in a cool and dark place for later use.
[...
example
[0070] Microbial challenge test of a certain whitening skin care lotion with antiseptic system compounded with methylparaben, propylparaben and honeysuckle extract:
[0071] 1. Take a sample of 315ml, and add 45ml each to seven 100ml Erlenmeyer flasks evenly for use; take 7 glass test tubes each containing 10ml sterilized saline for use, and 42 glass test tubes each containing 9ml saline Test tube spare.
[0072] 2. Take 6 kinds of pure strains (the 3rd to 7th generation activated), scrape one ring of each pure strain, add them to 6 glass test tubes, vibrate to make a uniform pure strain solution, and then Scrape one ring of the pure strains and add them to the remaining 1 glass test tube to prepare a uniform mixed strain solution for future use. The storage time of the whole process should not exceed 3 hours.
[0073] 3. Take 5ml each from the glass test tubes that have been prepared into a uniform strain solution, add them to a spare conical flask with 45ml of sample soluti...
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Abstract
The present invention relates to a method for resting and evaluating capacity of corrosion prevention system of cosmetic. The invention selects micrococcus pyogenes, bacillus coil, bacillus pyocyaneus, candida albicans, aspergillus niger and salmonella as evaluating strain. The product is grafted into the cosmetic sample solution through the six pathogens and the mixed strains composed of the six pathogens for executing culture experiment. The corrosion prevention capacity of cosmetic is evaluated through comparing and identifying data. The risk resistance capacity of cosmetic for resisting the microbial pollution is effectively evaluated. As the six pathogens have wide representativeness and typical reference value, the six pathogens are used as the typical representative sample strains for evaluating. If the corrosion prevention capacity test is passed, the pollution of product by the microorganism can be effectively prevented, and the possibility of product pollution caused by the reproduction of microorganism can be effectively suppressed. The safety risk caused by product and the cost for frequently executing microbiological examination can be reduced through the basic product formula checked by the corrosion prevention capacity test or all products extended from the basic product formula. The effect is extraordinarily remarkable.
Description
technical field [0001] The invention relates to a test and evaluation method for ensuring safe production of cosmetics, in particular to a test and evaluation method for the ability of a cosmetic antiseptic system. Background technique [0002] With the continuous improvement of people's living standards, people pay more attention to the maintenance of daily life. In daily life, cosmetics are used in a wider range, more and more users, and more and more product categories, from washing and bathing products to skin care and makeup products, covering the whole body from head to toe. Cosmetics manufacturers also range from large to small, with tens of thousands from south to north, with different production environments, product formulas, and raw materials used; product quality varies, but safety is always a crucial indicator; therefore , the anti-corrosion ability of the product is the most important factor related to the shelf life of the product, and it is also a crucial fa...
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