43 results about "Bacillus pyocyaneus" patented technology

The present invention belongs to the field of environment protecting technology, and is especially one kind of environment protecting fibrous membrane material. The environment protecting fibrous membrane material consists of nanometer TiO2 of 1-55 nm diameter and micron and nanometer fiber of 30-1500 nm diameter, and has pore diameter of 7-350 nm. The environment protecting fibrous membrane material can filter out micron level dust and other impurity, and nanometer level virus and microbes, eliminate chlorohydrocarbon, chlorine-containing organic and other toxic compound, reduce carcinogenic Cr6+ into non-toxic Cr3+, oxidize cyanide into non-toxic CO2, etc, kill Bacillus pyocyaneus, colibacillus, etc, and shield static electricity and ultraviolet ray.

The invention provides a pathogen inspection reagent, and concretely discloses a primer probe combination and a kit for combined inspection of 15 kinds of respiratory tract infection pathogens. By aiming at specific target sequences of the gene sequence conserved region of klebsiella pneumoniae, haemophilus influenzae, streptococcus pyogenes, staphylococcus aureus, escherichia coli, chlamydia pneumoniae, mycobacterium tuberculosis, stenotrophomonas maltophilia, baumanii, mycoplasma pneumoniae, enterococcus faecalis, ligionella pneumohpillia, streptococcus pneumoniae, bacillus pyocyaneus and mycobacterium abscessus, primers and probes which do not have mutual crossed reaction are designed, so that the problem that detection probes of different pathogens can easily generate mutual influenceor interference is solved; the combination and matching of different primers and probes are ensured; the goal of simultaneously performing efficient specific inspection at the same temperature can also be achieved.

The present invention relates to gene chip for detecting several kinds of common bacterial pathogens, and the gene chip includes solid carrier and oligonucleotide probe fixed on the carrier. The chip has up to 200 low density distributed sample points. The oligonucleotide detecting probe includes DNA' s selected from nucleotide sequences corresponding to the genomes of nine kinds of common bacterial pathogens, including Staphylococcus aureus, Staphylococcus epidermidis, bacillus pyocyaneus, colibacillus, etc. The said chip together with sample treating reagent, hybridizing reagent, color reagent and the specification constitutes the detection kit. The present invention has high detection efficiency.

The present invention relates to a method for resting and evaluating capacity of corrosion prevention system of cosmetic. The invention selects micrococcus pyogenes, bacillus coil, bacillus pyocyaneus, candida albicans, aspergillus niger and salmonella as evaluating strain. The product is grafted into the cosmetic sample solution through the six pathogens and the mixed strains composed of the six pathogens for executing culture experiment. The corrosion prevention capacity of cosmetic is evaluated through comparing and identifying data. The risk resistance capacity of cosmetic for resisting the microbial pollution is effectively evaluated. As the six pathogens have wide representativeness and typical reference value, the six pathogens are used as the typical representative sample strains for evaluating. If the corrosion prevention capacity test is passed, the pollution of product by the microorganism can be effectively prevented, and the possibility of product pollution caused by the reproduction of microorganism can be effectively suppressed. The safety risk caused by product and the cost for frequently executing microbiological examination can be reduced through the basic product formula checked by the corrosion prevention capacity test or all products extended from the basic product formula. The effect is extraordinarily remarkable.

The invention relates to an antibacillus pyocyaneus drug screening model which selects bacillus pyocyaneus external discharge pump adventitial protein MexAB-OprM as bull's-dot and to a method of screening antibacillus pyocyaneus drug using the said screening model. The invention also relates to a recombination bacillus pyocyaneus of excess expression MexAB-OprM protein which can be used in the said screening model and the screening method. The invention also relates to the antibacillus pyocyaneus drug obtained by the said screening model and method.

The invention aims to provide a multiple PCR detection kit and a method for identifying susceptible bacteria species for intracranial post-operation to eliminate endogenous nucleic acid contamination. The detected bacteria species are the following common bacteria: staphylococcus aureus, enterococcus faecalis, streptococcus pneumoniae, escherichia coli, bacillus pyocyaneus, klebsiella pneumoniae and acinetobacter baumannii. Meanwhile, the invention provides the method to eliminate the endogenous nucleic acid contamination in a raw material of a PCR system and exclude a false positive experimental result of a PCR caused by the endogenous nucleic acid contamination. The detecting method using the kit has the advantages of that the operation is simple and fast, the detection cost is lowered, the detection result is accurate, and the false positive result is reduced. A negative control and a positive control are added in the amplification step, so that the judgment of false positive and false negative is effectively improved, and the detection accuracy is higher.

The invention discloses a polyurethane material with a protein adsorption resistant function and an antibacterial function. The polyurethane material consists of a modification layer and a base layer; the base layer is composed of a common commercial polyurethane material, the modification layer is formed on the surface of the base layer by a chemical modification method, and the modification layer contains fungi carboxymethyl-pachymaran derivative CP which is bonded with the polyurethane base layer by a covalent bond. The preparation of the polyurethane material comprises the following steps: preparing the fungi carboxymethyl-pachymaran derivative CP; functionalizing the surface of a PU material by MDI; grafting an amino on the surface of the PU material; and grafting the fungi carboxymethyl-pachymarand derivative CP on the surface of the PU material. The material has better hydrophilicity and protein adsorption resistant function, as well as the antibacterial function of inhibiting bacillus pyocyaneus, thus the material has the use as a biomedical material of an artificial organs and a part implanted into human body. The material has simple and easily controlled preparation method and low cost.

The invention discloses a post-neurosurgical intracranial bacterial infection common pathogenic bacterium MLPA detection probe and an application thereof. The probe comprises a first group of probes to a seventeenth group of probes, and the first group of probes to the seventeenth group of probes are probes for detecting Neisseria meningitides, Bacillus pyocyaneus, Salmonella, Stenotrophomonas maltophilia, Staphylococcus aureus, Staphylococcus epidermidis, Klebsiella pneumonia, Serratia marcescens, Acinetobacter lwoffii, Corynebacteria diphtheria, Streptococcus mitis, Acinetobacter baumannii, Streptococcus pneumonia, Propionibacterium acnes, Listeria monocytogenes, Enterobacter cloacae and Haemophilus influenza sequentially. The probe is developed through an MLPA technology, can rapidly diagnose post-neurosurgical intracranial bacterial infection 17 common pathogenic bacteria once, provides possibility for rapid diagnosis of pathogenic bacteria of like patients, and is helpful for reducing the fatality and the disability rate of like infection.

The invention relates to a design, a preparation and an application of iodine antibacterial hydroxyapatite coating metal implants. The metal implants comprise pure titanium or a titanium alloy matrix and an iodine antibacterial hydroxyapatite coating which comprises hydroxyapatite and povidone iodine particles, wherein iodine is used as an antibacterial additive, and the mass percentage of iodine in the composite coating is 1-3%. The matrix which is pretreated is subjected to a vacuum plasma spraying technology to prepare the composite coating. The composite coating which has the bone ingrowth characteristic of prosthese surface hydroxyapatite and has the antibacterial performance of iodine has a good biocompatibility, a good biomechanical performance and an antibacterial performance, can control the infection and the loosening related with the implants of prosthese and the like, and simultaneously avoids the toxic side effect caused by the whole body antibiotic application and possible adverse events of the drug resistance and the like caused by the local antibiotic application. A titanium alloy femoral joint prosthese example of the hydroxyapatite composite coating containing 2wt% of iodine is shown in Fig. 1. The inhibition rate of hydroxyapatite coatings of different iodine contents to escherichia coli, Bacillus pyocyaneus and staphylococcus aureus is shown in Fig. 2.

The present invention provides a Chinese medicine preparation for killing virus and its production process. Said production process includes the following steps: decocting the Chinese medicinal materials of sweet wormwood, bupleurum root, epimedium, cranesbill, mistletoe and licorice by adding water, filtering to obtain filtrate 1; pressing ginger and garlic bulb, filtering to obtain filtrate 2; uniformly mixing filtrate 1 and filtrate 2 according to weight portion to obtain the invented liquid medicine for killing virus; also can extract the filtrate 1 and filtrate 2 by using alcohol, concentrating and drying so as to obtain solid powder preparation. Said liquid medicine can be directly used for killing several viruses and various fungi of staphylococcus aureus, pneumococcus, salmonella, salmonella typhi, dysentery bacillus, bacillus pyocyaneus, streptococcus and vibrio cholerae, etc.

The invention discloses a Pseudomonas aeruginosa vaccine and a preparation method thereof, which relate to a vaccine and a preparation method thereof. The invention solves the problems that the prior antibiotic is difficult to cure Pseudomonas aeruginosa infection, and a Pseudomonas aeruginosa vaccine with single valence can not satisfy the demand of clinical immunity. The vaccine consists of endotoxin of Pseudomonas aeruginosa P1, endotoxin of Pseudomonas aeruginosa P2, endotoxin of Pseudomonas aeruginosa P6 and toxoid of Pseudomonas aeruginosa P103 which are placed in physiological saline. The preparation method comprises the following steps: the endotoxins of Pseudomonas aeruginosaes are prepared; the toxoid of the Pseudomonas aeruginosa P103 is prepared; and the Pseudomonas aeruginosa vaccine can be obtained by mixing the endotoxins of the Pseudomonas aeruginosaes and the toxoid of the Pseudomonas aeruginosa P103. The Pseudomonas aeruginosa vaccine belongs to a polyvalent vaccine; the endotoxins of the Pseudomonas aeruginosa P1, the Pseudomonas aeruginosa P2 and the Pseudomonas aeruginosa P6 are adopted, so known type 11 Pseudomonas aeruginosa can be immunized; and the toxoid of the Pseudomonas aeruginosa P103 is added, so the immune effect of the vaccine to the Pseudomonas aeruginosaes is strengthened.

The present invention provides a dampness-eliminating itching-relieving Chinese medicine preparation with the action of inhibiting dermatomyces, trichophyton violaceum, trichophyton concentricum, achorion schoenleini and bacteria of staphylococcus aureus, hemolytic streptococcus, colibacillus, bacillus pyocyaneus and bacillus proteus, etc. for curing dermatosises with obvious therapeutic effect. Said Chinese medicine preparation is made up by using 10 Chinese medicinal materials of kochia fruit, cnidium seed, silkworm, dictamnus root bark, capillaries, schizonepeta spike and others through a certain preparation process.

The invention relates to a kit for detecting presence of pathogen pseudomonas aeruginosa in patient samples such as clinical bacterial pneumonia, corneal ulcer, urinary tract infection, septicemia and the like and samples such as cosmetics, environmental monitoring objects and the like, in particular to a kit for early and quickly diagnosing pseudomonas aeruginosa infection by a real-time fluorescence quantitative polymerase chain reaction technique.

The invention relates to a pharmaceutical composition for treating inflammation and process for preparation, wherein the process consists of steps of obtaining main constituents of the medicament through diacolation extracting, charging pharmaceutically acceptable auxiliary materials, making medicinal preparation through the conventional process. The obtained medicament can be used for treating inflammation caused by bacillus pyocyaneus infection, burns and scalds.

The suppository for treating bacterial vaginitis contains total phellodendron alkaloid and volatile atractylodes rhizome oil in the weight ratio of 1 to 1.6. The suppository has the effects of resisting bacteria, resisting fungi, resisting vagina trichomonad, resisting inflammation, relieving pain and stopping itch and can reach the effect of curing bacterial vaginitis radically. Pharmacological experiments show that the suppository comprehensive curative effect higher than that of any one kind of chemical medicine. The suppository has obvious inhibiting and deactivating effect on common vagina infecting bacteria, such as escherichia coli, Staphylococcos aureus, bacillus pyocyaneus, etc. Pharmacological experiments proves the use of the composition of total phellodendron alkaloid and volatile atractylodes rhizome oil in treating bacterial vaginitis.

Disclosed is an imine-resistant bacillus pyocyaneus bacteriophage and its use for treating infection, wherein a stain of broad-spectrum drug-resistant bacillus pyocyaneus bacteriophage phiA392, which can effectively kill drug-resistant bacillus pyocyaneus in vivo and in vitro, the bacteriophage can decompose multiple strains of clinically segregated imipenem-resistant bacillus pyocyaneus in vivo. The invention proves the superiority and feasibility of bacteriophage as a therapeutic substance in the clinical application to the treatment of bacillus pyocyaneus resistant affection, and provides a novel therapeutic means for clinical imipenem-resistant bacillus pyocyaneus affection.

The invention discloses a chemical synthesis method of Bacillus pyocyaneus 011 serotype O antigen oligosaccharide, and belongs to the field of chemistry. The method comprises the steps that a D-glucose block, an L-fucosamine block and a D-fucosimide block are utilized to construct O antigen trisaccharide, wherein the D-glucose block or the L-fucosamine block is connected with the D-fucosimide block through a 1,2-alpha-cis-glycosidic bond, the D-glucose block is connected with the L-fucososamine block through a 1,2-alpha-trans-glycosidic bond, and the 1,2-alpha-cis-glycosidic bond is constructed in a mixed solvent; the mixed solvent comprises two or more of dichloromethane, diethyl ether and thiophene. The method has the advantages that D-mannose is utilized as a raw material to obtain D-fucose simply, conveniently and efficiently; the uniform construction of the cis-glycosidic bond is achieved by relying on the appropriate mixed solvent; the stereoselectivity can reach 100%; and the method has a good application prospect in the development of novel drugs and vaccines for resisting Bacillus pyocyaneus and the like.

The present invention relates to medicine technology, and the fimbriate orostachys herb suppository is prepared with the effective components of fimbriate orostachys herb and excipient. Clinical application probes its bacteriostasis on staphylococcus aureus, colibacillus, bacillus pyocyaneus and streptococcus as common bacteria in vagina. It has the main functions of clearing away heat, promoting diuresis, promoting blood circulation to disperse blood clots, removing necrosis and promoting granulation, and is used mainly in cervical erosion caused by wetness-heat evil attacking the lower warmer. The present invention has simple composition and high treating effect.

The present invention is one kind of prepared recombinant targeted toxin, M4G3 / ScFv-PE40, for the targeting treatment of breast cancer, and belongs to the field of biotechnology. The recombinant targeted toxin M4G3 / ScFv-PE40 is prepared through fusing and co-expressing mouse anti-human breast cancer single strand antibody M4G3-scFv and Bacillus pyocyaneus epitoxin PE40. The recombinant targeted toxin can recognize and combine with T47D human breast cancer cell specifically in dose dependence, and has obvious cytotoxicity on T47D human breast cancer cell in dose-effect relationship and highest inhibiting rate up to 42.63 %, so that it may be applied in biological treatment of breast cancer.

The invention relates to an animal influenza target drug and relative production. Wherein, it uses single colon antibody to select the cross oncocyte seed which can resist animal influenza virus and inflammatory facto, and it can extract different cross oncocytes, via RT-PCT to obtain heavy chain variable area and light chain variable area genes with single antibody; and via SOE-PCT, to connect the VH, VL genes with one short peptide with 15 amino acid, to build the single chain antibody gene of single chain antibody gene, or single chain dual special antibody gene, and inflammatory facto, and connect the single chain antibody gene or single chain dual special antibody gene, via enzyme cutting to connect the single chain antibody gene or bacillus pyocyaneus ectotoxin PE40 gene of inflammatory facto, to build original core represent carrier, transfer Ecoli.BL21 projective virus, then via ferment, extraction, and purification, to obtain target antagonist.

The invention relates to a special flora for sewage treatment. The special flora is prepared from the following components in percentage by mass: 10 to 15 percent of pseudomonas aeruginosa, 13 to 20 percent of bacillus, 5 to 10 percent of rhodococcus sp, 3 to 8 percent of acinetobacter, 8 to 15 percent of bacillus pyocyaneus, 15 to 21 percent of bacillus alcaligenes, 3 to 5 percent of lactobacillus acidophilus, 1 to 6 percent of Streptomyces and 10 to 25 percent of coral nocardia, wherein the mass sum of the strains is 100 percent. A cultivation method for the special flora for the sewage treatment, disclosed by the invention, has the advantages of simple operation and broad conditions; the special flora has better treatment effects on sulfate, organic matters and sewage with higher content of NH3-N; the treated sewage which meets relevant national emission standards can be directly discharged or recycled; besides, the special flora has the advantages of low sludge quantity, zero secondary pollution, stable treatment effect, environment friendliness and zero toxicity.

The invention discloses a water-soluble microbial agent for promoting banana growing, a preparation method and application thereof. The compound contains six microorganisms, including pseudomonas aeruginosa, serratia marcescens, burkholderia tuberum, pseudomonas fluorescens, bacillus subtilis and trichoderma viride. The preparation method comprises the following steps of: preparing a water-soluble carbon source from rice flour processing wastes; culturing the six bacteria in the water-soluble carbon source, thereby obtaining six liquid microbial agents; and airing and mixing the six liquid microbial agents, thereby obtaining the water-soluble compound microbial agent. The invention relates to the technical fields of waste utilization, agricultural microorganisms, ecological planting and microbial agents. According to the water-soluble microbial agent for promoting banana growing, the preparation method and the application thereof, the rice flour wastes can be recycled, the industrial production cost can be lowered, the waste of waste resources can be avoided, the environmental pollution caused by the wastes can be reduced, the yield of banana can be increased and the plant diseases and insect pests of banana can be reduced.

The invention relates to a surfing message bathtub. It comprises a message pump connected with circulating pipe, and a message injector installed in bathtub; the circulating pipe is equipped with a nanometer photo-catalytic filter device, which comprises a filter drum installed in circulating pipe, said filter drum is equipped with a nanometer photo-catalytic antibiotic foam metal filter net, and a baffle board, said metal filter net is equipped between the baffle board. The baffle board is arranged in axial and layered type, while said metal filter net is arranged along circumference. The baffle board is multi-layer and is equipped with water hole, and water hole in near baffle board is installed in two sides of said metal filter net. The filter drum is installed in the circulating pipe, and water in bathtub is drove by message pump to be treated by said metal filter net for half an hour, and the killing rate of bacillus coli, Micrococcus pyogenes, bacillus pyocyaneus, and bacillus pyocyaneus amounts to 99.5%.

The present invention relates to gliocladin and its application, and belongs to the field of medical antibiotic technology. The gliocladin is obtained with the production strain and through conventional liquid or solid fermentation and extraction of the metabolite. The production strain is Gliocladium roseum Gr87 with preservation number of CGMCC No. 0807. The gliocladin has molecular expression of C21H16O2, and molecular weight 384. The gliocladin is used in preparing antibiotic for Shigella sonnei, tubercle mycobacillus, bacillus pyocyaneus, Shigella shigae, etc.

The invention relates to a composite medicine of non-viral vector fused protein TNF alpha m2-SON2-GR and limiting expression bacillus pyocyaneus exotoxin gene killing functional domain high-efficiency mutant type recombinant 5xNFBS-SCP-PEIIImut. In the non-viral vector, the TNF alpha m2 is a strong-efficiency and low-toxicity TNF alpha derivative, SON2 is a DNA combined protein, and GRP is gastrin release peptide; the 5 x NFBS of the toxin gene recombinant is the combined site of NF-kappa B repeatedly cloned for 5 times, SCP is a tumor specificity promoter, and PEIIImut is the bacillus pyocyaneus exotoxin gene killing functional domain high-efficiency mutant type. The non-viral vector can combine and carry the toxin gene recombinant to enter positive cells of a GRP acceptor, but the toxin gene can only be expressed in positive malignant tumor cells of the NF-kappa B<+>SCP <+>GRP acceptor, and further strongly efficient kills the tumor cells insensitive to chemical treatment and radioactive treatment. The non-viral vector can independently kill the positive malignant tumor cells of the GRP acceptor. The invention also relates to application of the composite medicine and the independent non-viral vector in the treatment of the malignant tumor.

The invention relates to a surfing message bathtub. It comprises a message pump connected with circulating pipe, and a message injector installed in bathtub; the circulating pipe is equipped with a nanometer photo-catalytic filter device, which comprises a filter drum installed in circulating pipe, said filter drum is equipped with a nanometer photo-catalytic antibiotic foam metal filter net, and a baffle board, said metal filter net is equipped between the baffle board. The baffle board is arranged in axial and layered type, while said metal filter net is arranged along circumference. The baffle board is multi-layer and is equipped with water hole, and water hole in near baffle board is installed in two sides of said metal filter net. The filter drum is installed in the circulating pipe, and water in bathtub is drove by message pump to be treated by said metal filter net for half an hour, and the killing rate of bacillus coli, Micrococcus pyogenes, bacillus pyocyaneus, and bacillus pyocyaneus amounts to 99.5%.