The invention discloses a post-neurosurgical intracranial bacterial infection common pathogenic bacterium MLPA detection probe and an application thereof. The probe comprises a first group of probes to a seventeenth group of probes, and the first group of probes to the seventeenth group of probes are probes for detecting Neisseria meningitides, Bacillus pyocyaneus, Salmonella, Stenotrophomonas maltophilia, Staphylococcus aureus, Staphylococcus epidermidis, Klebsiella pneumonia, Serratia marcescens, Acinetobacter lwoffii, Corynebacteria diphtheria, Streptococcus mitis, Acinetobacter baumannii, Streptococcus pneumonia, Propionibacterium acnes, Listeria monocytogenes, Enterobacter cloacae and Haemophilus influenza sequentially. The probe is developed through an MLPA technology, can rapidly diagnose post-neurosurgical intracranial bacterial infection 17 common pathogenic bacteria once, provides possibility for rapid diagnosis of pathogenic bacteria of like patients, and is helpful for reducing the fatality and the disability rate of like infection.