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Nianzhou Strain and its use

A technology of stickomycin and stickomyces pink, which is applied in antibacterial drugs, biochemical equipment and methods, microorganisms, etc., and can solve problems such as undiscovered public reports

Inactive Publication Date: 2006-12-06
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Through literature search, do not find the public report identical with the present invention

Method used

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  • Nianzhou Strain and its use
  • Nianzhou Strain and its use

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Extracting and separating the compound of the present invention from the solid fermented product of Glioma pink, the specific steps are as follows:

[0032] a. Seed culture of the fungus Gliocladium roseum Gr87:

[0033] Improved PDA medium: 200g of potato, 20g of glucose, 1.0g of yeast extract, 6.0g of glycerol, 17g of agar, 1000mL of water, make a test tube slant, pick strains into the slant, and culture at 28°C for 5 days;

[0034] b. Solid fermentation culture of the fungus Glioma pink:

[0035] Wheat culture medium (the preparation process is: soak the wheat in tap water for 48 hours, then wash it, drain the water, put it into a 500cm Erlenmeyer flask, wrap the mouth of the bottle with cotton cloth and sterilize twice at 15psi, 40min each time).

[0036] Pick the strain cultured on the slant into the solid fermentation medium, and culture it statically at room temperature 25°C for 30 days;

[0037] c. freeze-dry the mycelium of the above-mentioned cultured Gliocl...

Embodiment 2

[0042] Disc method was used to detect the effect of cocladin on eight pathogens including Shigella sonnii, Mycobacterium tuberculosis, Pseudomonas aeruginosa, Shigella shigellii, Staphylococcus aureus, Escherichia coli, hemolytic streptococcus and Shigella parashigella Bacterial antagonistic activity test.

[0043] 1. Test drug

[0044] Dissolve the test sample in a small amount of organic reagent DMSO, and then add a certain concentration of Tween aqueous solution to prepare sample test solutions with a concentration of 200ppm. In this solution, the final concentration of DMSO is <3%, and the final concentration of Tween is <5‰. In addition, the same concentration of DMSO was dissolved in an aqueous solution containing 0.5% (V / V) Tween-20 as a control.

[0045] 2. Cultivation of pathogenic bacteria

[0046] The formula of the culture medium is conventional beef extract peptone medium (beef extract 3g, peptone 10g, NaCl 5g, water 1000mL, pH 7.5), inoculate the pathogenic bact...

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Abstract

The present invention relates to gliocladin and its application, and belongs to the field of medical antibiotic technology. The gliocladin is obtained with the production strain and through conventional liquid or solid fermentation and extraction of the metabolite. The production strain is Gliocladium roseum Gr87 with preservation number of CGMCC No. 0807. The gliocladin has molecular expression of C21H16O2, and molecular weight 384. The gliocladin is used in preparing antibiotic for Shigella sonnei, tubercle mycobacillus, bacillus pyocyaneus, Shigella shigae, etc.

Description

Technical field: [0001] The invention relates to a muscomycin and its application, and belongs to the technical field of medical antibiotics. Background technique: [0002] So far, nearly ten thousand kinds of antibiotics derived from microorganisms have been discovered, many of which have been widely used in the treatment of various microbial infectious diseases. In recent years, due to the resistance of pathogenic fungi and pathogenic bacteria, and the mutation of viruses, the therapeutic effect of the original medical antibiotics and antiviral antibiotics is declining. It is necessary to continuously develop new antibiotics, and the invention of new active ingredients of antibiotics is the key to the problem. [0003] Through document search, do not find the public report identical with the present invention. Invention content: [0004] The purpose of the present invention is to provide a kind of colistin with novel chemical structure, which can be used as the preparat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D487/14C12P17/18A61P13/04C12R1/645A61K31/55A61P31/04A61P31/06
CPCY02A50/30
Inventor 董锦艳张克勤
Owner YUNNAN UNIV
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