Primer probe combination and kit for combined inspection of 15 kinds of respiratory tract infection pathogens

A primer-probe, pathogen technology, applied in microorganisms, recombinant DNA technology, microorganism-based methods, etc., to achieve strong specificity, high-efficiency specific detection, and ensure matching effects

Active Publication Date: 2018-04-20
西安九安生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, there is currently no effective method that can simultaneously detect the a

Method used

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  • Primer probe combination and kit for combined inspection of 15 kinds of respiratory tract infection pathogens
  • Primer probe combination and kit for combined inspection of 15 kinds of respiratory tract infection pathogens
  • Primer probe combination and kit for combined inspection of 15 kinds of respiratory tract infection pathogens

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Embodiment 1

[0114] This example is used to illustrate a kit for specifically detecting 15 kinds of respiratory pathogenic bacteria in a sample. Its components include PCR reaction solution, enzyme mixture, negative control substance and positive quality control substance. The PCR reaction solution mainly contains relevant Primers and probes, reaction buffer, Mg 2+ And dNTPs, the enzyme mixture mainly contains hot-start Taq enzyme, the negative control product is RNase and DNase-free water, and the positive quality control product is a recombinant plasmid containing 15 kinds of pathogen detection target sequences.

[0115] The primers and probes used for nucleic acid amplification in the PCR reaction solution are listed in Table 1. They are specific primers designed for the specific conservative sequences of 15 respiratory pathogenic bacteria genome groups and screened out by pre-experiment and used for fluorescence signal monitoring Oligonucleotide probes, and primers and probes for reference...

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Abstract

The invention provides a pathogen inspection reagent, and concretely discloses a primer probe combination and a kit for combined inspection of 15 kinds of respiratory tract infection pathogens. By aiming at specific target sequences of the gene sequence conserved region of klebsiella pneumoniae, haemophilus influenzae, streptococcus pyogenes, staphylococcus aureus, escherichia coli, chlamydia pneumoniae, mycobacterium tuberculosis, stenotrophomonas maltophilia, baumanii, mycoplasma pneumoniae, enterococcus faecalis, ligionella pneumohpillia, streptococcus pneumoniae, bacillus pyocyaneus and mycobacterium abscessus, primers and probes which do not have mutual crossed reaction are designed, so that the problem that detection probes of different pathogens can easily generate mutual influenceor interference is solved; the combination and matching of different primers and probes are ensured; the goal of simultaneously performing efficient specific inspection at the same temperature can also be achieved.

Description

Technical field [0001] The invention relates to a reagent for detecting pathogens, in particular to a primer-probe combination and a kit for joint detection of 15 respiratory tract infection pathogens. Background technique [0002] Among adults and children, fever with respiratory syndrome is the most common acute infectious disease, which is mainly caused by local respiratory tract infections or lesions of tissues and organs outside the respiratory tract caused by a variety of pathogens, including bacteria, viruses, mycoplasma, and chlamydia. [0003] Since the clinical symptoms are all manifested as fever (body temperature ≥38℃), accompanied by fatigue, headache, cough, sore throat, body aches and other upper respiratory symptoms, the diagnosis of various pathogens requires a large number of experimental screenings, and the operation steps are cumbersome, It takes a long time and the work efficiency is low. [0004] At present, common pathogens of community respiratory infections ...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/686C12Q1/04C12N15/11C12R1/22C12R1/21C12R1/46C12R1/445C12R1/19C12R1/01C12R1/32C12R1/35C12R1/38
CPCC12Q1/686C12Q1/689C12Q2561/113C12Q2561/101
Inventor 不公告发明人
Owner 西安九安生物技术有限公司
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