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Method and kit for quantitative combined detection of PA (Prealbumin) and CRP (C-reactive Protein), as well as preparation method and application of kit

A combined detection and kit technology, applied in the field of medical testing, can solve the problems of single evaluation index and low clinical acceptance.

Inactive Publication Date: 2017-05-17
北京中生金域诊断技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the nutritional diagnostic in vitro diagnostic products currently on the market have a single evaluation index, and the clinical acceptance is very low

Method used

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  • Method and kit for quantitative combined detection of PA (Prealbumin) and CRP (C-reactive Protein), as well as preparation method and application of kit
  • Method and kit for quantitative combined detection of PA (Prealbumin) and CRP (C-reactive Protein), as well as preparation method and application of kit
  • Method and kit for quantitative combined detection of PA (Prealbumin) and CRP (C-reactive Protein), as well as preparation method and application of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1 Preparation of the kit for quantitative joint detection of PA and CRP in samples

[0062] In this example, the bottom plate material is PVC plastic sheet, which is purchased from Yuyao Fucheng Plastic Chemical Co., Ltd.; the material of the sample pad is glass fiber film, which is purchased from Shanghai Jinbiao Biotechnology Co., Ltd.; the material of the bonding pad is glass The fiber membrane was purchased from Shanghai Jinbiao Biotechnology Co., Ltd. Anti-PA antibody I and anti-PA antibody II have different antigen-binding sites, and anti-CRP antibody I and anti-CRP antibody II have different antigen-binding sites.

[0063] (1) Preparation of nitrocellulose membrane

[0064] Use PB buffer (with 3% sucrose) at pH 7.2 to 7.4 to adjust the concentration of anti-PA antibody II to 0.5 mg / mL, and the concentration of donkey anti-mouse antibody to 1.0 mg / mL, using a Bio-Dot XYZ3050 three-dimensional spraying platform Coat the anti-PA antibody II and donkey anti...

Embodiment 2

[0071] Quantitative detection of PA and CRP in the sample of embodiment 2

[0072] Use the test strip prepared in embodiment 1 to detect PA and CRP in the sample, and its steps include:

[0073] (1) Add solutions containing PA standards with different known concentrations (specifically 0.0, 2.5, 5.0, 12.5, 25.0, 37.5, 75.0, 100, 200 μg / L) to the sample well of the sample pad 1, in the sample Add solutions containing CRP standards with different known concentrations (specifically 0.0, 1.0, 2.5, 5.0, 7.5, 10.0, 50.0, 100.0, 200.0, 400.0 μg / L) to the sample well of pad II, and react for 15 minutes. Place the test strip under a 470nm excitation light source, measure the fluorescence intensity of the strips on the detection line I and quality control line I, and the fluorescence intensity of the strips on the detection line II and quality control line II respectively, according to the detection line I and the quality control line respectively. The ratio of the fluorescence intensi...

Embodiment 3

[0075] Quantitative detection of prealbumin and C-reactive protein in the sample of Example 3

[0076] Take the test strip prepared in Example 1, open the aluminum foil bag and take out the test strip, place it flat on the table, take 2 μL of blood sample in the CRP reagent bottle filled with 2mL of 0.01M pH 7.4 phosphate buffer solution, mix thoroughly and separate Take 100μL and add it to the CRP reagent bottle filled with 0.9mL 0.01M pH 7.4 phosphate buffer solution, take 100uL solution in the reagent bottle and add it to the sample hole of the test strip corresponding to the detection of PA and CRP. After 15 minutes of reaction, put the test paper Place the strips under the excitation light source, measure the fluorescence intensity of the strips on the detection line I and quality control line I, and the fluorescence intensity of the strips on the detection line II and quality control line II respectively, and compare the detection line I and the quality control line I Th...

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Abstract

The invention provides a method and a kit for quantitative combined detection of PA (Prealbumin) and CRP (C-reactive Protein), as well as a preparation method and application of the kit. The kit comprises a test paper strip I and a test paper strip II, which are used for detecting PA and CRP, wherein the test paper strip I is composed of a bottom plate I, a sample pad I, a combination pad I, a nitrocellulose membrane I and water absorption paper I; and the test paper strip II is composed of a bottom plate II, a sample pad II, a combination pad II, a nitrocellulose membrane II and water absorption paper II. The detection method comprises the following steps: (1) adding a PA standard object into the sample pad I and adding a CRP standard object into the sample pad II; after reacting, drawing a standard curve according to a specific value of the fluorescence intensity of a detection line and the fluorescence intensity of a quality control line; and (2) adding a solution of an object to be detected into the sample pad I and the sample pad II, and reading PA content and CRP content in the solution of the object to be detected according to the standard curve. The kit and the detection method, provided by the invention, are simple and convenient to operate, high in clinical acceptance degree and good in detection effect.

Description

technical field [0001] The invention belongs to the technical field of medical testing, and in particular relates to a method for quantitative joint detection of PA and CRP, a kit, a preparation method and application thereof. Background technique [0002] The low nutritional diagnosis rate of inpatients and outpatients is a common phenomenon in our country's medical services. According to statistics, the nutritional diagnosis rate is less than 1% among nearly 100 million inpatients in my country every year. Based on the recognized malnutrition rate of 40% of inpatients, 40 million inpatients suffering from malnutrition do not receive reasonable and effective nutritional support every year, which affects their clinical outcomes. [0003] Malnutrition and organ function damage are a gradual process, and the metabolic characteristics are different in different stages. Therefore, the evaluation of nutritional and metabolic status and organ function evaluation must use multi-in...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/533G01N21/64
CPCG01N33/6893G01N21/6428G01N33/533G01N2021/6439
Inventor 李超辉王加义凌燕王维赖卫华熊勇华
Owner 北京中生金域诊断技术股份有限公司
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