3922results about "Heating or cooling apparatus" patented technology

A sample holder includes a first member featuring a first retaining mechanism configured to retain a first substrate that includes a sample, a second member featuring a second retaining mechanism configured to retain a second substrate that includes a reagent medium, and an alignment mechanism connected to at least one of the first and second members, and configured to align the first and second members such that the sample contacts at least a portion of the reagent medium when the first and second members are aligned.

Described herein are digital microfluidic (DMF) devices and corresponding methods for managing reagent solution evaporation during a reaction. Reactions on the DMF devices described here are performed in an air or gas matrix. The DMF devices include a means for performing reactions at different temperatures. To address the issue of evaporation of the reaction droplet especially when the reaction is performed at higher temperatures, a means for introducing a replenishing droplet has been incorporated into the DMF device. A replenishing droplet is introduced every time when it has been determined that the reaction droplet has fallen below a threshold volume. Detection and monitoring of the reaction droplet may be through visual, optical, fluorescence, colorimetric, and / or electrical means.

The invention relates to the field of environment simulation control, in particular to an environment simulation method and system for a high and low temperature test box. The method comprises the following steps: acquiring initial state monitoring parameters of a high and low temperature test box, performing regional environment parameter division and time point gradient change fitting, and constructing a plurality of regional temperature and humidity gradient change curves; identifying a test object in the high and low temperature test box; performing transient thermal change limit evolution excavation on the test object according to the plurality of regional temperature and humidity gradient change curves to generate a transient thermal change limit curve of the current test object; acquiring a preset test box environment simulation log, performing global temperature change cross period optimization according to the transient thermal change limit curve of the current test object, and constructing a global temperature change optimization simulation period; and executing environment simulation control operation according to the global temperature change optimization simulation period, and collecting high and low temperature simulation environment monitoring data. According to the invention, quick-response, accurate and stable environment simulation control is realized.

The invention discloses a hot air type qPCR instrument, a multi-channel detection optical system and a detection method, and relates to the field of molecular biological detection. The problems that an optical detection system adopted by an existing qPCR instrument mostly depends on a single-channel or double-channel design, sample hole sites need to be switched one by one and detected in a time-sharing mode in the detection process, namely, the efficiency is low in the face of a high-throughput requirement scene are solved, and the system combines multiple LED light sources with an optical filter to generate excitation wavelengths to form a light source module; after the multi-stage dichroscope is utilized to form a light combination module, the light combination module is transmitted to a reaction hole through one branch of the forked optical fiber to excite fluorescent substances, a fluorescent signal is collected through a common end of the forked optical fiber, the other branch of the forked optical fiber outputs a fluorescent signal, and the fluorescent signal is split by the reflection grating to form a light splitting module. And the array CMOS is combined with a spectrum unmixing algorithm to form a detection module for synchronous receiving, so that multi-channel simultaneous high-sensitivity detection is realized. The method is also suitable for the field of multi-pathogen detection and gene expression analysis.

The invention discloses a frequency converter performance detection device, and relates to the technical field of frequency converters, the frequency converter performance detection device comprises a detection box, a heat-conducting fin is arranged in the detection box, and the heat-conducting fin is of a four-side square structure and forms a sealed cold and hot channel on the edge of an inner cavity of the detection box; the multiple damping shock absorbers are arranged at the bottom of the detection box; the vibration generator is arranged at the lower part of the detection box and is used for providing a vibration source for the detection box; the output end of the humidifier is communicated with the inner cavity of the detection box; and the output end of the cold-hot generator communicates with the cold-hot channel. The vibration generator, the humidifier and the cold and heat generator are arranged, the detection box is matched, multifunctional simulation of vibration, high-humidity, high-temperature and low-temperature environments in the peripheral environment is achieved, stability detection of the frequency converter under the extreme environment is achieved, and the cold and heat channel in a circulating circulation state is formed in the inner edge of the detection box; cold and hot air covers the detection box for multi-surface circulation, so that the distribution uniformity of the cold and hot air is improved.

The invention provides a weather-proof test system for an abnormal sound vibration test. The weather-proof test system comprises an environment box and abnormal sound test equipment, the environment box comprises a base table and a box body, a temperature adjusting chamber is formed in the box body, the base table is provided with a carrying base face located on the lower side of the temperature adjusting chamber, the base table is provided with a heat insulation chamber sunken relative to the carrying base face, and the heat insulation chamber is separated from the temperature adjusting chamber through a heat insulation plate; the abnormal sound testing equipment comprises a driving mechanism, a transmission mechanism and a bearing mechanism, the driving mechanism comprises a vibration exciter, the vibration exciter is arranged in the isolation chamber, and the transmission mechanism and the bearing mechanism are arranged above the carrying base plane. Different parts of the abnormal sound testing equipment are separated by arranging the temperature adjusting chamber and the heat insulation chamber, the transmission mechanism and the bearing mechanism of the mechanical part are arranged in the temperature adjusting chamber, and the driving mechanism of the electrical part is arranged in the heat insulation chamber, so that the requirement of a weather-proof test can be met, and the working stability of the vibration exciter can be ensured.

Provided herein are devices, methods, and systems for in situ gene sequencing of a target nucleic acid in a cell in an intact tissue. Methods of screening a candidate agent to determine whether the candidate agent modulates gene expression of a nucleic acid in a cell in an intact tissue are also provided herein.

The technology described herein generally relates to microfluidic cartridges configured to amplify and detect polynucleotides extracted from multiple biological samples in parallel. The technology includes a microfluidic substrate, comprising: a plurality of sample lanes, wherein each of the plurality of sample lanes comprises a microfluidic network having, in fluid communication with one another: an inlet; a first valve and a second valve; a first channel leading from the inlet, via the first valve, to a reaction chamber; and a second channel leading from the reaction chamber, via the second valve, to a vent.

Compositions for preventing or limiting surface fouling as well as evaporation and methods for their use in air-matrix digital microfluidics (DMF) apparatuses are described. A mobilizing wax material may be used to selectively encapsulate a reaction droplet in the air gap of the apparatus, which permits the at least partially encapsulated reaction droplet to be portable within the DMF apparatus. Additional aqueous droplets may be combined with the encapsulated droplet, by merging with an aqueous droplet having a coating of a secondary material (e.g., an oil or other hydrophobic material) that may allow combining of the droplets. The compositions may be additionally useful in non-DMF applications such as laboratory protocols for hybridization, ligation and amplification.

The utility model belongs to the technical field of temperature control equipment, and particularly relates to a gas-liquid double-cooling low-temperature thermostatic bath which comprises a machine body, a bath body and a refrigeration mechanism, and the bath body and the refrigeration mechanism are located in the machine body. The refrigerating mechanism is connected with a condensate coil pipe in the tank body, the tank body is connected with a liquid inlet and a liquid outlet, and liquid in the tank body circulates or circulates through the liquid inlet and the liquid outlet. A gas coil pipe is further arranged in the tank body, a gas inlet connector and a gas outlet connector are arranged on the side face of the machine body, and gas enters the gas coil pipe through a pipeline and the gas inlet connector and flows out of the gas outlet connector after being cooled by liquid in the tank body. The liquid and gas cooling device can meet the cooling requirements of liquid and gas, is suitable for wider experiment or industrial application scenes, is particularly applied to the working condition that the liquid and the gas are cooperatively cooled, has higher adaptability, can better keep the consistency and the stability of the temperature, and saves time and resources.

The invention discloses a photo-thermal amplification and real-time PCR (polymerase chain reaction) detection integrated optical fiber system, and relates to the field of pathogen testing, the optical fiber system comprises an optical fiber probe, a photo-thermal photosensitizer and a micro reaction chamber; the micro-reaction chamber is used for extracting and discharging a to-be-detected sample by utilizing air pressure, the optical fiber probe is arranged in the micro-reaction chamber, and the optical fiber probe reacts with the to-be-detected sample in the micro-reaction chamber; the photo-thermal photosensitizer is arranged inside or on the outer surface of the optical fiber probe and is used for receiving heat generated by the heating light to heat the optical fiber probe and controlling the reaction temperature of the optical fiber probe and the to-be-detected sample; the optical fiber probe is also used for receiving the exciting light which excites the to-be-detected sample to generate a fluorescence signal, and the optical fiber probe is used for detecting the fluorescence signal. According to the application, sample collection, photo-thermal cycle amplification and real-time fluorescent quantitative detection can be integrated, and rapid and effective detection of a virus sample is realized.

The invention relates to the field of equipment verification, in particular to a thermostatic bath and a thermostatic method. The constant temperature bath comprises at least one constant temperature bath unit, the magnetic stirrer is rotationally arranged at the inner bottom of the tank body, the cooling fan is arranged at the outer bottom of the tank body, the magnet is arranged on the cooling fan and rotates along with the cooling fan, the semiconductor chilling plate wraps the periphery of the tank body and the periphery of the cooling fan, and the cooling fin is attached to the semiconductor chilling plate. The size of the calorimeter temperature sensor calibrating device is reduced, the operation efficiency and the automation degree are improved, and the temperature can be uniformly controlled.

A cell culture system includes: a plurality of cell culture channels each of which includes a fluidic device in which cells are cultured, a pump which makes a liquid flow into the fluidic device, a detection unit which detects a state of the cells that are cultured, and a controller which controls the pump and the detection unit; a measurement unit that measures culture environment of the cells which are cultured in the plurality of cell culture channels; and an information processing apparatus that adjusts the culture environment such that an absolute value of a difference between evaluation values for the states of the cells that are cultured in the plurality of cell culture channels is a threshold value or less, on the basis of the state of the cells detected by the detection unit and the culture environment of the cells measured by the measurement unit.

The utility model belongs to the technical field of micro-fluidic chips, and particularly provides a digital MIRA real-time diagnosis chip and an analysis device.The digital MIRA real-time diagnosis chip comprises a top glass slide, a micro-pattern layer and a bottom glass slide which are sequentially arranged from top to bottom; a water-soluble film is embedded in a position where the micro-pattern layer is matched with the water filling nozzle; the sample injection port is suitable for sequentially injecting a reaction reagent and sealing oil. Through the design of a chip structure without an outlet, when reaction reagents are introduced from a sample injection port, all the reaction reagents simultaneously, equivalently and uniformly enter each micro-chamber, and the reaction reagents are separated into each micro-chamber by adopting an oil seal mode, so that the chip has the advantage of 100% sample discretization in the digital nucleic acid detection process, and no sample or reagent is lost. Meanwhile, each part of reaction reagent is accurately separated into each micro-chamber and cannot be pushed out of the chip, so that each molecule enters the corresponding micro-chamber, and the detection requirement of a low-concentration template is met.

The application discloses a pollution-preventing detection device, method and application for rapid detection of nucleic acid amplification, which comprises a detection part and a mixed reaction cabin, and the mixed reaction cabin comprises a reaction cabin main body and a reaction cabin cover which is in sealed connection with the reaction cabin main body and can be detached; a channel for connecting a hybridization liquid containing part and a mixed reaction part is arranged between the reaction cabin cover and the reaction cabin main body; the detection part comprises a detection inner core and a detection box body containing the detection inner core; a transparent observation window is arranged on the detection box body and corresponds to the reaction area position of the detection inner core; a flow guide device matched with the mixed reaction cabin is further arranged on the detection box body; the flow guide device comprises a flow guide pipe and a flow guide part; one end of the flow guide part is connected with the flow guide pipe, and the other end is connected with the detection inner core; the hybridization liquid and the sample mixed liquid are guided to the detection inner core; and the detection inner core displays the final detection result in the area corresponding to the transparent observation window. The application realizes that the liquid in the reaction tube flows out to the designated detection card in a completely closed environment, and has the advantages of good sealing effect and prevention of primer aerosol pollution.

A test chamber and a method for conditioning air in a test space thereof for receiving test material, the test space being configured to be sealed from an environment and temperature-insulated, a cooling device of a temperature control device of the test chamber, which comprises a cooling circuit with carbon dioxide as a refrigerant, a heat exchanger in the test space, a low-pressure compressor, and a high-pressure compressor, a gas cooler, and an expansion valve downstream of the low-pressure compressor in a flow direction of the refrigerant, being used to establish a temperature between −20° C. to +180° C. within the test space, a control device of the test chamber being used to control the temperature and / or a relative humidity in the test space. A dehumidifier bypass of the cooling circuit, which comprises a second expansion valve and a second heat exchanger, is used to dehumidify air in the test space.

The invention relates to a space experiment sample observation mechanism, a space experiment device and a fluid physics experiment cupboard, the space experiment sample observation mechanism comprises a plug connector, a sample sleeve and a first heating sheet, the first heating sheet is arranged on the sample sleeve, one end of the sample sleeve is fixed on the back side surface of the plug connector, and the other end of the sample sleeve is fixed on the back side surface of the plug connector; a sample clamping part is arranged at the other end of the sample sleeve; the sample clamping part comprises a circular-arc-shaped sample clamping piece with an opening in the upper side, a bottom connecting piece and a cylindrical sample clamping piece, the sample sleeve, the circular-arc-shaped sample clamping piece and the cylindrical sample clamping piece are coaxially arranged, and the sample sleeve, the circular-arc-shaped sample clamping piece, the bottom connecting piece and the cylindrical sample clamping piece are sequentially connected; the bottom of the arc-shaped sample clamping piece is fixedly connected with the bottom of the cylindrical sample clamping piece through a bottom connecting piece, a sample observation interval position is reserved between the arc-shaped sample clamping piece and the cylindrical sample clamping piece, and a sample observation opening position penetrating in the direction perpendicular to the axis of the cylindrical sample clamping piece is formed in the side wall of the cylindrical sample clamping piece.

The utility model relates to a rotary sample holder and an oven in the technical field of material testing. The rotary sample holder comprises a main shaft; the two circular ring fixers are coaxially arranged on the main shaft respectively, and the two circular ring fixers are arranged on the main shaft in a spaced mode; the plurality of sample holders are uniformly divided into two groups, and the two groups of sample holders are respectively mounted on the two circular ring fixers; the driver is arranged on the main shaft and used for driving the main shaft to rotate around the axis of the main shaft. The rotary sample holder can ensure that each group of samples are heated consistently and uniformly in the test process, and the accuracy of a rubber aging test result is improved.

This disclosure relates to apparatus and methods for molecular diagnostics. Certain embodiments include a piston cycled from a first position proximal to a first end of a housing, to a second position proximal to a second end of the housing, and back to the first position proximal to the first end of the housing. In some embodiments, the present disclosure relates to devices, methods, and systems for molecular diagnostics that do not comprise a piston.

To provide methods for selectively combining discrete entities including cells, reagents, drugs, hydrogels, extracellular matrices, beads, particles, biological materials, media, or a combination thereof.SOLUTION: The method comprises sorting a plurality of discrete entities and trapping two or more discrete entities for a time sufficient for the two or more discrete entities to combine to form a combined discrete entity. In certain aspects, the method comprises making the plurality of discrete entities. In certain aspects, the method comprises detecting or analyzing the discrete entities, for example, via optical detection. In certain aspects, the method comprises manipulating or analyzing the combined discrete entity or a component therein, for example, imaging, sequencing, culturing, for example, three-dimensional culturing, and measuring cell-cell interactions.SELECTED DRAWING: None

A microfluidic device includes a plurality of reaction wells; and a plurality of solid supports, and each of the solid supports has a reagent attached thereto. The reagent is attached to the solid support via a labile reagent / support bond such that the reagent is configured to be cleaved from the support via a cleaving operation.

Provided herein are systems, and methods configured to optimize a system for sequencing one or more nucleic acid samples.

The invention discloses an experiment box for raw material medicine azacitidine, and relates to the technical field of experiment equipment, the experiment box comprises a box body and a sealing device, the left side of the box body is fixedly provided with a temperature control device, the left side of the temperature control device is fixedly provided with a button, the button is electrically connected with the temperature control device, and the front side of the box body is provided with a replacement hole. A replacing plate is slidably mounted on the front side of the box body, a sealing plate slidably penetrates through the right side of the box body, the sealing device comprises a limiting hole, a limiting rod, a limiting spring, a U-shaped rod, a T-shaped plate, a stabilizing plate and a net-shaped frame, and after an experimental container containing azacitidine is placed into the box body, the sealing plate is manually pulled to move leftwards to reset and recover sealing of the box body; when the azacitidine is degraded, moisture in air can enter the box body due to loosening of the sealing plate, so that degradation of azacitidine is accelerated, it can be ensured that the sealing plate is always tightly closed through mistaken touch prevention limitation of the T-shaped plate, and therefore the stability of azacitidine is ensured.

A PCR kit-of-parts is described. The kit-of-parts comprises a first oligonucleotide bound to a bead by a cleavable linker; a second oligonucleotide bound to a bead by a cleavable linker; and an enzyme bound to a bead by a cleavable linker; wherein the first and second oligonucleotide form an oligonucleotide pair complementary to a nucleic acid of interest and the enzyme is capable of extending nucleic acid strands; and each cleavable linker is independently selected from a photocleavable linker and a thermally cleavable linker. Also described is a method of performing PCR and a PCR system.

The invention relates to a temperature control method and device and nucleic acid amplification detection equipment. The method comprises the following steps: when a main heating element reaches a target working temperature and the temperature is stable, obtaining a current combination corresponding to each auxiliary heating element according to the target working temperature and a preset corresponding relation; wherein the preset corresponding relation is the relation between the temperature and the current of the auxiliary heating piece meeting the temperature uniformity requirement; each auxiliary heating element is arranged corresponding to each temperature compensation area; and according to the current combination, an auxiliary heating element is driven to carry out temperature compensation adjustment on the temperature compensation area. The temperature uniformity can be realized, and the temperature accuracy is ensured.

The invention relates to the technical field of oil product detection, in particular to a full-automatic condensation point determinator and a determination method.The full-automatic condensation point determinator comprises a base and a test tube, the top of the base is fixedly connected with a mounting base, the surface of the base and the surface of the mounting base are provided with a test bath mechanism and a supporting mechanism, and the test bath mechanism enables a motor to drive a test bath to rotate; the rotation angle of the motor is controlled through a preset program, precise positioning is achieved, the tedious operation of hole-by-hole adjustment of the original structure is avoided, the rotating shaft is locked through mechanical clamping, angle deviation in the testing process is prevented, then manual adjustment is replaced by the motor and the electric push rod, and the test efficiency is improved. The double locking effect is achieved, accidental displacement can be prevented, and precision and efficiency are improved; according to the clamping mechanism, the four arc-shaped clamping plates apply uniform radial pressure through the first springs to clamp the outer wall of the test tube, the inner diameters of the clamping plates are gradually increased from top to bottom, and the clamping mechanism is compatible with test tubes with different outer diameters.

A fluid cassette for a fluid management system, including a housing defining a fluid pathway therethrough, an inflow tubing extending from the housing, and an outflow tubing extending from the housing. The fluid pathway includes a fluid dampening chamber having a fluid inlet configured for fluid ingress into the fluid dampening chamber and a fluid outlet configured for fluid egress from the fluid dampening chamber, where an upper extent of the fluid outlet is lower than an upper extent of the fluid inlet. The fluid pathway also includes a bifurcated fluid pathway including a first branch of the bifurcated fluid pathway and a second branch of the bifurcated fluid pathway, and a mixing fluid channel for mixing heated fluid exiting the bifurcated fluid pathway.