2561results about "Heating or cooling apparatus" patented technology

The invention relates to the field of environment simulation control, in particular to an environment simulation method and system for a high and low temperature test box. The method comprises the following steps: acquiring initial state monitoring parameters of a high and low temperature test box, performing regional environment parameter division and time point gradient change fitting, and constructing a plurality of regional temperature and humidity gradient change curves; identifying a test object in the high and low temperature test box; performing transient thermal change limit evolution excavation on the test object according to the plurality of regional temperature and humidity gradient change curves to generate a transient thermal change limit curve of the current test object; acquiring a preset test box environment simulation log, performing global temperature change cross period optimization according to the transient thermal change limit curve of the current test object, and constructing a global temperature change optimization simulation period; and executing environment simulation control operation according to the global temperature change optimization simulation period, and collecting high and low temperature simulation environment monitoring data. According to the invention, quick-response, accurate and stable environment simulation control is realized.

The invention discloses a hot air type qPCR instrument, a multi-channel detection optical system and a detection method, and relates to the field of molecular biological detection. The problems that an optical detection system adopted by an existing qPCR instrument mostly depends on a single-channel or double-channel design, sample hole sites need to be switched one by one and detected in a time-sharing mode in the detection process, namely, the efficiency is low in the face of a high-throughput requirement scene are solved, and the system combines multiple LED light sources with an optical filter to generate excitation wavelengths to form a light source module; after the multi-stage dichroscope is utilized to form a light combination module, the light combination module is transmitted to a reaction hole through one branch of the forked optical fiber to excite fluorescent substances, a fluorescent signal is collected through a common end of the forked optical fiber, the other branch of the forked optical fiber outputs a fluorescent signal, and the fluorescent signal is split by the reflection grating to form a light splitting module. And the array CMOS is combined with a spectrum unmixing algorithm to form a detection module for synchronous receiving, so that multi-channel simultaneous high-sensitivity detection is realized. The method is also suitable for the field of multi-pathogen detection and gene expression analysis.

The utility model belongs to the technical field of temperature control equipment, and particularly relates to a gas-liquid double-cooling low-temperature thermostatic bath which comprises a machine body, a bath body and a refrigeration mechanism, and the bath body and the refrigeration mechanism are located in the machine body. The refrigerating mechanism is connected with a condensate coil pipe in the tank body, the tank body is connected with a liquid inlet and a liquid outlet, and liquid in the tank body circulates or circulates through the liquid inlet and the liquid outlet. A gas coil pipe is further arranged in the tank body, a gas inlet connector and a gas outlet connector are arranged on the side face of the machine body, and gas enters the gas coil pipe through a pipeline and the gas inlet connector and flows out of the gas outlet connector after being cooled by liquid in the tank body. The liquid and gas cooling device can meet the cooling requirements of liquid and gas, is suitable for wider experiment or industrial application scenes, is particularly applied to the working condition that the liquid and the gas are cooperatively cooled, has higher adaptability, can better keep the consistency and the stability of the temperature, and saves time and resources.

A cell culture system includes: a plurality of cell culture channels each of which includes a fluidic device in which cells are cultured, a pump which makes a liquid flow into the fluidic device, a detection unit which detects a state of the cells that are cultured, and a controller which controls the pump and the detection unit; a measurement unit that measures culture environment of the cells which are cultured in the plurality of cell culture channels; and an information processing apparatus that adjusts the culture environment such that an absolute value of a difference between evaluation values for the states of the cells that are cultured in the plurality of cell culture channels is a threshold value or less, on the basis of the state of the cells detected by the detection unit and the culture environment of the cells measured by the measurement unit.

The utility model belongs to the technical field of micro-fluidic chips, and particularly provides a digital MIRA real-time diagnosis chip and an analysis device.The digital MIRA real-time diagnosis chip comprises a top glass slide, a micro-pattern layer and a bottom glass slide which are sequentially arranged from top to bottom; a water-soluble film is embedded in a position where the micro-pattern layer is matched with the water filling nozzle; the sample injection port is suitable for sequentially injecting a reaction reagent and sealing oil. Through the design of a chip structure without an outlet, when reaction reagents are introduced from a sample injection port, all the reaction reagents simultaneously, equivalently and uniformly enter each micro-chamber, and the reaction reagents are separated into each micro-chamber by adopting an oil seal mode, so that the chip has the advantage of 100% sample discretization in the digital nucleic acid detection process, and no sample or reagent is lost. Meanwhile, each part of reaction reagent is accurately separated into each micro-chamber and cannot be pushed out of the chip, so that each molecule enters the corresponding micro-chamber, and the detection requirement of a low-concentration template is met.

The application discloses a pollution-preventing detection device, method and application for rapid detection of nucleic acid amplification, which comprises a detection part and a mixed reaction cabin, and the mixed reaction cabin comprises a reaction cabin main body and a reaction cabin cover which is in sealed connection with the reaction cabin main body and can be detached; a channel for connecting a hybridization liquid containing part and a mixed reaction part is arranged between the reaction cabin cover and the reaction cabin main body; the detection part comprises a detection inner core and a detection box body containing the detection inner core; a transparent observation window is arranged on the detection box body and corresponds to the reaction area position of the detection inner core; a flow guide device matched with the mixed reaction cabin is further arranged on the detection box body; the flow guide device comprises a flow guide pipe and a flow guide part; one end of the flow guide part is connected with the flow guide pipe, and the other end is connected with the detection inner core; the hybridization liquid and the sample mixed liquid are guided to the detection inner core; and the detection inner core displays the final detection result in the area corresponding to the transparent observation window. The application realizes that the liquid in the reaction tube flows out to the designated detection card in a completely closed environment, and has the advantages of good sealing effect and prevention of primer aerosol pollution.

The invention relates to a space experiment sample observation mechanism, a space experiment device and a fluid physics experiment cupboard, the space experiment sample observation mechanism comprises a plug connector, a sample sleeve and a first heating sheet, the first heating sheet is arranged on the sample sleeve, one end of the sample sleeve is fixed on the back side surface of the plug connector, and the other end of the sample sleeve is fixed on the back side surface of the plug connector; a sample clamping part is arranged at the other end of the sample sleeve; the sample clamping part comprises a circular-arc-shaped sample clamping piece with an opening in the upper side, a bottom connecting piece and a cylindrical sample clamping piece, the sample sleeve, the circular-arc-shaped sample clamping piece and the cylindrical sample clamping piece are coaxially arranged, and the sample sleeve, the circular-arc-shaped sample clamping piece, the bottom connecting piece and the cylindrical sample clamping piece are sequentially connected; the bottom of the arc-shaped sample clamping piece is fixedly connected with the bottom of the cylindrical sample clamping piece through a bottom connecting piece, a sample observation interval position is reserved between the arc-shaped sample clamping piece and the cylindrical sample clamping piece, and a sample observation opening position penetrating in the direction perpendicular to the axis of the cylindrical sample clamping piece is formed in the side wall of the cylindrical sample clamping piece.

This disclosure relates to apparatus and methods for molecular diagnostics. Certain embodiments include a piston cycled from a first position proximal to a first end of a housing, to a second position proximal to a second end of the housing, and back to the first position proximal to the first end of the housing. In some embodiments, the present disclosure relates to devices, methods, and systems for molecular diagnostics that do not comprise a piston.

To provide methods for selectively combining discrete entities including cells, reagents, drugs, hydrogels, extracellular matrices, beads, particles, biological materials, media, or a combination thereof.SOLUTION: The method comprises sorting a plurality of discrete entities and trapping two or more discrete entities for a time sufficient for the two or more discrete entities to combine to form a combined discrete entity. In certain aspects, the method comprises making the plurality of discrete entities. In certain aspects, the method comprises detecting or analyzing the discrete entities, for example, via optical detection. In certain aspects, the method comprises manipulating or analyzing the combined discrete entity or a component therein, for example, imaging, sequencing, culturing, for example, three-dimensional culturing, and measuring cell-cell interactions.SELECTED DRAWING: None

The invention relates to a temperature control method and device and nucleic acid amplification detection equipment. The method comprises the following steps: when a main heating element reaches a target working temperature and the temperature is stable, obtaining a current combination corresponding to each auxiliary heating element according to the target working temperature and a preset corresponding relation; wherein the preset corresponding relation is the relation between the temperature and the current of the auxiliary heating piece meeting the temperature uniformity requirement; each auxiliary heating element is arranged corresponding to each temperature compensation area; and according to the current combination, an auxiliary heating element is driven to carry out temperature compensation adjustment on the temperature compensation area. The temperature uniformity can be realized, and the temperature accuracy is ensured.

The invention relates to the technical field of oil product detection, in particular to a full-automatic condensation point determinator and a determination method.The full-automatic condensation point determinator comprises a base and a test tube, the top of the base is fixedly connected with a mounting base, the surface of the base and the surface of the mounting base are provided with a test bath mechanism and a supporting mechanism, and the test bath mechanism enables a motor to drive a test bath to rotate; the rotation angle of the motor is controlled through a preset program, precise positioning is achieved, the tedious operation of hole-by-hole adjustment of the original structure is avoided, the rotating shaft is locked through mechanical clamping, angle deviation in the testing process is prevented, then manual adjustment is replaced by the motor and the electric push rod, and the test efficiency is improved. The double locking effect is achieved, accidental displacement can be prevented, and precision and efficiency are improved; according to the clamping mechanism, the four arc-shaped clamping plates apply uniform radial pressure through the first springs to clamp the outer wall of the test tube, the inner diameters of the clamping plates are gradually increased from top to bottom, and the clamping mechanism is compatible with test tubes with different outer diameters.

The invention relates to a microfluidic nucleic acid detection device, and relates to the field of detection. Comprising a bottom plate, a chip inlet and outlet module erected on one side above the bottom plate, a chip table erected on the chip inlet and outlet module and used for bearing a micro-fluidic chip with a liquid bag, a displacement module erected above the chip table, and a crushing module erected on the displacement module and used for squeezing liquid in the liquid bag into the micro-fluidic chip, the liquid transferring module is erected on one side of the crushing module and is used for transferring liquid in the micro-fluidic chip to flow; the pneumatic module is erected above the front end of the bottom plate and is used for controlling a flow channel of the micro-fluidic chip to be opened and closed; the heating module is erected on the other side above the bottom plate and is used for locally heating the micro-fluidic chip and automatically detecting the temperature; and the fluorescent module is used for acquiring a fluorescent signal of the micro-fluidic chip. The micro-fluidic chip detection device has the advantages that different types of micro-fluidic chips can be rapidly detected, and an accurate result can be obtained.

The invention discloses ultrafast photo-thermal PCR amplification equipment and a use method. The ultrafast photo-thermal PCR amplification equipment comprises a shell, and a laser probe and an infrared temperature sensor are arranged in the shell; a chip rack is arranged in the shell, and a chip to be tested is placed in the chip rack; the laser probe is located on one side of the chip frame, the infrared temperature sensor is located on the other side of the chip frame, and the laser probe inclines upwards in the vertical direction. An embedded industrial all-in-one machine is installed on the shell, and the output end of the infrared temperature sensor is connected with the input end of the embedded industrial all-in-one machine. An excitation light source of the laser probe and a coupling non-contact infrared temperature measurement sensor can realize temperature control of nanoparticles in different batches, the ultrahigh temperature change requirement of PCR is met, infrared temperature measurement control replaces a traditional thermocouple to serve as a temperature feedback device, the phenomenon of reagent pollution caused by contact measurement is avoided, and the detection accuracy is improved. The accuracy of later data detection is ensured, and the laser probe is inclined upwards along the vertical direction, so that a PCR reaction area is fully covered.

The invention discloses a temperature control system and method of a micro-fluidic chip, and relates to the technical field of micro-fluidic temperature control, and the method comprises the following steps: in the manufacturing process of the micro-fluidic chip, embedding topological insulator nanowires into a substrate in a directional arrangement mode to form a pre-locking array, and meanwhile, generating an anisotropic heat conduction network under the continuous action of a sound-magnetic field, a topological vortex invariant is calculated, and a heat conduction weight coefficient is generated; arranging a fluorescent layer on the surface of a micro-reaction cavity of the anisotropic heat conduction network, collecting a fluorescence lifetime attenuation curve in real time, and calculating a transient temperature value and a temperature change rate according to a heat conduction constraint variation model and a heat conduction weight system; nonlinear feed-forward compensation power is calculated according to the transient temperature value and the temperature change rate, feedback compensation power is calculated in combination with a deviation field of the target temperature and the real-time temperature, and a compensation power instruction is generated; according to the method, the thermal pilot frequency domain filtering function is constructed through the compensation power instruction, so that the uniformity and response speed of thermal response are improved.

Provided is an integrated nucleic acid extraction and amplification testing device, comprising a bracket, an amplification apparatus, an extraction apparatus, a fluorescence detection apparatus and a driving apparatus. The amplification apparatus is disposed on the bracket, and is used to perform temperature adjustment; the extraction apparatus is mounted on the amplification apparatus, and the extraction device comprises a test tube mounting assembly, a first driving member and a magnetic attraction component, the test tube mounting assembly having a first mounting hole, the magnetic attraction component being disposed on a hole wall of the first mounting hole, and the first driving member being used to drive the test tube mounting component to rotate, thereby driving the magnetic attraction component to rotate around an outer peripheral side of a test tube located in the test tube mounting assembly and perform nucleic acid extraction; the fluorescence detection apparatus is mounted on the bracket, and is used to perform fluorescence detection of the test tube; an output end of the driving apparatus is connected to the amplification apparatus, and is used to drive the amplification apparatus and the extraction apparatus to rotate relative to the fluorescence detection apparatus. By integrating the functions of nucleic acid extraction, amplification and fluorescence detection into a single device, efficiency is improved and testing time is shortened.

This disclosure provides for devices, methods, and systems for generating a plurality of droplets within a collecting container at an extremely high rate (e.g., of at least 1 million droplets per minute, etc.), each of the plurality of droplets comprising an aqueous mixture for a digital analysis, wherein upon generation, the plurality of droplets is stabilized in position within a region of the collecting container. The inventions enable partitioning of samples for digital analyses at unprecedented rates, where readout of signals from targets within such partitions can still be achieved in accordance with various assays.

The invention relates to an environmental test box for realizing high and low temperature rapid alternating circulation, which is mainly applied to the technical field of environmental test equipment. The test box is provided with a high-temperature chamber and a low-temperature chamber which are independent from each other, a thermal insulation layer is arranged between the two chambers, and the test box can respectively keep constant temperature environments at the highest temperature of + 75 DEG C and the lowest temperature of-50 DEG C; during a test, a sample is placed in the test bed, and the rotating shaft structure driven by the motor periodically rotates in a reciprocating manner or rotates in the same direction among different temperature zones, so that high-low temperature rapid alternating circulation is realized. The operation mode of the motor is controlled through the control system so as to simulate the accelerated aging process of the component in the temperature rapid alternating environment. According to the test box, through the design of the control system, the forced convection fan and the middle thermal insulation layer, it is guaranteed that the temperature switching process is rapid, uniform and stable. Compared with the prior art, high and low temperature circulation can be completed without overall heating and cooling of the interior of the test box, and the test box has the advantages of being high in test efficiency, capable of remarkably improving the reliability and accuracy of the high and low temperature alternating circulation test of the sample and the like.

The invention relates to a space microscopic sample support, a space experiment device and a fluid physics experiment cupboard, the space microscopic sample support comprises a support body and a heating sheet, one side surface of the support body is provided with the heating sheet, the other side surface of the support body is recessed to form a sample slot, and the heating sheet is arranged in the sample slot. And an observation light path penetrating through the bottom of the sample slot is arranged on the bracket body. According to the space microscopic sample support, a microscopic sample is taken out by an astronaut and placed on the sample support during observation, the sample support can maintain the liquid state of the microscopic sample during observation, and temperature uniformity is ensured.

The invention relates to a space experiment sample temperature processing device and a space fluid physics experiment cabinet, and the space experiment sample temperature processing device comprises a housing, an alternating temperature on-orbit sample drawer, a constant temperature on-orbit sample drawer, an alternating temperature descending sample drawer and a constant temperature descending sample drawer. An alternating temperature on-orbit sample cavity, a constant temperature on-orbit sample cavity, an alternating temperature descending sample cavity and a constant temperature descending sample cavity are arranged in the shell, an alternating temperature on-orbit sample drawer is arranged in the alternating temperature on-orbit sample cavity, and a constant temperature on-orbit sample drawer is arranged in the constant temperature on-orbit sample cavity; the alternating temperature descending sample drawer is arranged in the alternating temperature descending sample cavity, and the constant temperature descending sample drawer is arranged in the constant temperature descending sample cavity; the shell is provided with a temperature regulating mechanism for regulating and controlling the temperature of the alternating temperature on-orbit sample drawer, the constant temperature on-orbit sample drawer, the alternating temperature descending sample drawer and the constant temperature descending sample drawer.

A semiconductor structure and a microfluidic system comprising the semiconductor structure are disclosed. The semiconductor structure comprises a thermoelement layer. The thermoelement layer comprises p- and n-type thermoelements. These thermoelements form regions wherein respective region is associated with a specific temperature range, where achieving the specific temperature range is based on an electron or hole current flowing through the thermoelements. The semiconductor structure forms part of the microfluidic system comprising a microfluidic channel having a meander extension across regions having different temperature ranges. This allows a fluid flowing in the microfluidic channel being exposable to cyclic temperature variations.

The utility model belongs to the technical field of test boxes, and particularly relates to a high-low temperature test box with uniform heating. Comprising test cavities which are arranged in a test box at intervals left and right; a placement net plate is embedded in the bottom of the test cavity; the fans are arranged below the placing net plate in a bilateral symmetry manner; the air inlet end of the fan is communicated with the test cavity through the placing screen plate, and the air outlet end of the fan is communicated with the air duct cavity; the air duct cavities are distributed on the left side wall and the right side wall of the test cavity in a bilateral symmetry manner; a spoiler is vertically and obliquely arranged in the air duct cavity, and an air distribution plate is arranged at the air return end of the air duct cavity. According to the utility model, through the arrangement of the spoiler and the air distribution plate in the air duct cavity, high and low temperature airflow with uniform airflow distribution can be provided for the interior of the test cavity, so that the temperature rise rates of different areas are consistent, and the uniform heating effect during product testing is further improved.

The invention discloses a magnetic aggregation induced emission particle fluorescence-electrochemical nucleic acid dual detection chip and system.The chip comprises a chip body and an upper cover, and a plurality of electrochemical reaction detection cavities and fluorescence reaction detection cavities are formed in the left side and the right side of the chip body in the front-back direction respectively; the electrochemical reaction detection cavities and the fluorescence reaction detection cavities are arranged in a one-to-one correspondence manner; each electrochemical reaction detection cavity and each fluorescence reaction detection cavity are provided with a flow channel, and one end of each flow channel is communicated with the electrochemical reaction detection cavity or the fluorescence reaction detection cavity; the other end of the sampling tube is communicated with an MAIE electrochemical detection auxiliary liquid sample adding hole and an MAIE fluorescence detection auxiliary liquid sample adding hole which are formed in the chip main body; a plurality of MAIE electrochemical detection sample adding holes and MAIE fluorescence detection sample adding holes are formed in the upper cover, and corresponding openings are formed in the positions, corresponding to the MAIE electrochemical detection auxiliary liquid adding holes and the MAIE fluorescence detection auxiliary liquid adding holes, of the upper cover.

The utility model discloses a temperature-controllable asphalt concrete non-contact slope flow instrument device, which comprises a temperature control box and an angle adjusting device, the angle adjusting device comprises a bottom support, an adjusting plate and a base, the left end of the adjusting plate is rotatably connected with the bottom support, the base is arranged below the adjusting plate, a sample table is arranged on the upper surface of the base, and the sample table is connected with the temperature control box. The sample table penetrates to the position above the surface of the adjusting plate, a sample is arranged on the sample table, an adjusting screw is arranged on the upper surface of the base and penetrates to the position above the adjusting plate, an adjusting nut is arranged on the adjusting screw, and a heat insulation glass box is arranged on one side of the upper surface of the adjusting plate. A rechargeable battery, an angle display and a laser displacement sensor are arranged in the heat insulation glass box; according to the structure, an independent heat insulation glass box is adopted, a laser hole and a heat insulation film are arranged, and the heat insulation film is used for reducing errors, caused by refraction, of a laser beam penetrating through the high-temperature-resistant heat insulation glass box on the sample placement position and deformation data of the sample.

The invention discloses equipment and a method for detecting the thermal insulation performance of an integrated thermal insulation board, and relates to the field of detection equipment. According to the invention, the integrated insulation board is clamped in the detection box in a sealing manner through the sealing clamping assembly, so that the integrated insulation board divides the interior of the detection box into a left sealing chamber and a right sealing chamber, and then the sealing chamber on the left side of the interior of the detection box is heated or cooled through the temperature regulation and control mechanism. Then, the temperature of the left sealing cavity is detected through a temperature measuring probe in the left sealing cavity, meanwhile, the temperature of the right sealing cavity is detected through a temperature measuring probe in the right sealing cavity, and then the heat preservation performance of the integrated heat preservation plate is judged according to the temperature difference between the left sealing cavity and the right sealing cavity. Therefore, the thermal insulation performance of the integrated thermal insulation board can be detected by simulating the actual use condition of the integrated thermal insulation board, so that the thermal insulation performance detection accuracy of the integrated thermal insulation board is improved.

The invention relates to a modular storage system for a laboratory refrigerator. The object of the present invention is to specify a storage system for a laboratory refrigerator, which storage system is suitable for storing reagents, carrying out tests and storing test results, can be produced simply and cost-effectively, is easy to handle and can be easily stored and transported in good order, and in particular can receive the standard laboratory vessels flexibly and by hand within the storage system, in a way that can be quickly reorganized if necessary, and yet still permits defined sample positions in order to allow inventories to be taken in an efficient manner. The object is achieved by the system comprising the following individual components: a refrigerator box (1) consisting of a base (11), a side wall (12) which is provided on its outer face with a handle (121) and can be closed by a lid (13) so as to enclose an interior (14), at least one lifter (2) with hooks (21) and at least one module block (3) for receiving and holding reaction vessels, wherein the lifter (2) and at least one module block (3) can be placed in the interior of the refrigerator box, the lifter (2) makes it possible to carry at least one module block (3), in which the lifter (2) is formed in an L shape and has hooks (21) at its upper edge, and the side wall (12) of the refrigerator box (1) is provided with slits (122) at its upper edge for suspending the hooks (21) of the lifter (21), in order to position the lifter (2) in the interior (14) of the refrigerator box (1), and the lid (13) and the base (11) each have, on their outer faces directed away from the interior (14), a matching locking structure (4) of such form that at least one depression (413) on the top of the lid (13) and at least one border (411) on the base (11) of the refrigerator box (1) are provided which allow locking when several refrigerator boxes (1) are stacked one on top of another, by the depression (413) and the border (411) engaging in each other.

Provided herein are kits, systems and methods for analyzing analytes from a biological sample. The system includes an upper housing configured to receive a sample substrate and a thermal element configured to control a temperature of the sample substrate. The system includes a lower housing and an alignment mechanism coupling the upper housing to the lower housing. The alignment mechanism is configured to move the upper housing, the lower housing, or both into an arrangement having an open configuration, a closed configuration, or an intermediate configuration therebetween. The thermal element is configured to cool the sample substrate.

A laboratory sample distribution system is provided comprising: a number of container carriers, wherein the container carriers each comprise a magnetically active device and are adapted to carry a sample container; at least one fan being adapted to cause an airflow; and a number of transport modules, the transport modules being arrangeable adjacent to one another and respectively comprising: a transport surface being adapted to carry the container carriers; a number of heat dissipating electromagnetic actuators being stationary arranged below the transport surface, the electromagnetic actuators being adapted to move container carriers placed on top of the transport surface by applying magnetic forces to the container carriers; and an air guiding element being adapted to guide the airflow first towards an underside of the transport surface and afterwards towards the electromagnetic actuators.

The utility model discloses a comprehensive test box which comprises a foundation, a downwards-sunken foundation pit is arranged in the foundation, a concrete bearing base body is poured in the foundation pit, and a first buffer layer, a framework layer and a second buffer layer are arranged between the concrete bearing base body and the foundation pit. The first buffer layer, the framework layer and the second buffer layer are all of a cylindrical structure and wrap the circumferential direction and the bottom of the concrete bearing base body, and the framework layer is provided with a supporting part extending upwards in the vertical direction to form a foundation pit; the vibration table is fixedly mounted at the upper end of the concrete bearing base body through a metal base; the bottom of the test box body is provided with a butt joint window, and the butt joint window is fixedly connected to the supporting part in a sealed mode. The utility model has the beneficial effect of solving the technical problem that the connection sealing difficulty of the large-scale vibration table and the box body is large.

The present document describes methods and systems for amplifying and quantifying amplification of a nucleic acid molecule, with a polymerase chain reaction (PCR) or a loop-mediated isothermal amplification (LAMP), by irradiating, with a heating activation light beam from a continuous wave laser a biological enzymatic reaction mixture in solution comprising a nucleic acid template, a polymerase enzyme, and chemically modified nanoparticles. Quantification of the amplification is achieved by irradiating the biological enzymatic reaction mixture during an annealing and / or elongation steps with an ultraviolet (UV) light source and measuring with a photodetector a transmission change in UV light transmission.