216results about "Measurements of magnetic beads labelled molecules" patented technology

Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.

A system and method are provided to detect target analytes based on magnetic resonance measurements. Magnetic structures produce distinct magnetic field regions having a size comparable to the analyte. When the analyte is bound in those regions, magnetic resonance signals from the sample are changed, leading to detection of the analyte.

Methods of producing and/or analyzing spectroscopically labeled proteins, e.g., proteins site-specifically labeled with NMR active isotopes, spin-labels, chelators for paramagnetic metals, and the like, are provided. The labeled proteins are produced in translation systems including orthogonal aminoacyl tRNA synthetase/tRNA pairs. Methods for assigning NMR resonances, e.g., methods using isotopically labeled proteins, are also provided.

An integrated microelectronic sensor is provided in a disposable flow membrane sensing device. The integrated sensors detect electromagnetic effect labels in flow detection zones above the sensor in the membrane. The labels are small particles that give off a detectable electromagnetic signal. They are commonly used for isolating and quantifying biochemical targets of interest. The sensors are fabricated using planar integrated circuit technologies. Sensors can detect labels of several types including magnetic, electric, and photonic. These types all have in common the fact that the sensor detects the label at a distance. Magnetoresistive sensors for detecting magnetic labels, and photodiodes for detecting photonic labels are described.

A system for using the sensors is described. There are disposable cartridges with a backing that supports the sensors and membrane is described. The integrated sensor in the cartridge is designed to be discarded after use. Also, label excitation sources are provided. The multi sensor array chip can be configured in order to detect labels in multiple zones, and to monitor progress of flow down a strip of membrane. These multiple label detection zones, using sandwich assay techniques, can quantify analyte concentration for many types of analytical samples. Also, the membrane can be micropatterned in order to provide multiple or unusually shaped flow paths.

Methods and apparatus for analyzing bioprocess fluids are provided. A plurality of particles coated with a plurality of capture agents having an affinity for one or more biological markers is combined with bioprocess fluid to form a plurality of analyte-particle complexes. The system also includes a transport arrangement for transporting the sample to a sensor surface, and optionally a magnetic field inducing structure constructed and arranged to establish a magnetic field at and adjacent to the sensor surface. The resonant sensor produces a signal corresponding to an amount of analyte-particle complexes that are bound to the sensor surface.

This invention features systems and methods for the detection of analytes, and their use in the treatment and diagnosis of disease.

The present invention relates to method and device for detecting changes of a magnetic response of at least one magnetic particle in a carrier fluid. The method comprises: using a measuring procedure comprising measuring the characteristic rotation time of said magnetic particle, said measuring procedure further involving measuring Brownian relaxation in said carrier fluid under influence of an external pulsed excitation magnetic field, and based on said influence of said external pulsed excitation magnetic field measuring a hydrodynamic volume of a particle or a change in a hydrodynamic volume of the particle change upon modification of an effective volume of the particle or its interaction with said carrier fluid by detecting change of magnetization of the particle with time by monitoring change of an output signal in detection coil.

A magnetic sensor device (1000) for sensing magnetic particles (15), the magnetic sensor device (1000) comprising a first magnetic field generator unit (12) excitable for generating a first magnetic field, a sensing unit (11) adapted for sensing a signal indicative of the presence of the magnetic particles (15) in the first magnetic field, a second magnetic field generator unit (1001) adapted for generating a second magnetic field affecting the sensing unit (11), and a processing circuitry (1002) adapted for processing the signal to form a digitized signal and adapted for feeding back the processed signal to the second magnetic field generator unit (1001).

A biosensor utilizing bio-functionalized magnetic nanoparticles is provided. An external magnetic field is applied to a suspension of magnetic nanoparticles. A linearly polarized incident light is applied to the suspension of magnetic nanoparticles. A photocurrent from polarized light scattering by bio-functionalized magnetic nanoparticles in liquid is detected. The magneto-optic sensing technique is applied to a micro-fluidic channel for rapid and sensitive detection with a small sample amount, and subsequent magnetic separation for detoxification. This technique is used for the detection of Brownian relaxation with time sweep as well as frequency sweep. The magneto-optical sensor enables rapidly detecting changes in local dynamic properties of the magnetic nanoparticles in liquids and magnetic modulation of ferromagnetic particles in liquid provides increased signal sensitivity.

Disclosed is a magnetic field detector having various structures that can be used as a high-density magnetic biosensor. An embodiment of the invention provides a magnetic field detector using a thin film for detecting magnetic beads. The magnetic field detector includes: a substrate; a magnetoresistive element that is formed on an upper surface of the substrate in a ring shape using the thin film; electrodes that are formed on the upper surface of the substrate to be connected to the magnetoresistive element; a protective layer that is formed on the magnetoresistive element and the electrodes; and a magnetic bead limiting layer that is formed on an upper surface of the protective layer to cover the entire surface of the magnetoresistive element and portions of the electrodes.

A magnetic sensor for identifying small superparamagnetic particles bonded to a substrate contains a regular orthogonal array of MTJ cells formed beneath that substrate. A magnetic field imposed on the particle, perpendicular to the substrate, induces a magnetic field that has a component within the MTJ cells that is along the plane of the MTJ free layer. If that free layer has a low switching threshold, the induced field of the particle will create resistance changes in a group of MTJ cells that lie beneath it. These resistance changes will be distributed in a characteristic formation or signature that will indicate the presence of the particle. If the particle's field is insufficient to produce the free layer switching, then a biasing field can be added in the direction of the hard axis and the combination of this field and the induced field allows the presence of the particle to be determined.

A method and system are described for measuring agglutination in a target-induced agglutination assay with one or more magnetic particles performed in a reaction chamber. After the magnetic particles (3, 15), which are capable of binding to a target (5) are provided in the assay, an agglutination process resulting in agglutinated particles (100) comprising at least one magnetic particle is performed. The method then further comprises applying an alternating current magnetic field (H_AC) to the assay and—measuring an effect of the H_AC on the one or more magnetic particles (3,15) unattached to any surface. The measured effect is indicative of one or more agglutination parameters.

The present invention provides a magnetic sensor which detects a target substance indirectly by making a labeling substance larger than the target substance bond with the target substance contained in a sample in a detection area, and detecting the labeling substance, comprising a capture area which is relatively easy to capture the target substance, and a non-capture area which is relatively hard to capture the target substance, on a surface of a member which is comprised in a detection area, wherein the capture area is surrounded by the non-capture area. Thereby, the sensor enables to detect comparatively accurately the number and concentration of substances which cannot be directly detected, and enables to be used for detection of various target substances.

The present invention provides a magnetic sensor device a first magnetic field generating means (21a, 21b) for attracting magnetic or magnetizable objects (22), e.g. magnetic particles, to a sensor surface (23) and a second magnetic field generating means (25) for, in combination with the first magnetic field, repelling magnetic or magnetizable objects (22), e.g. magnetic particles, from the sensor surface (23). The magnetic fields generated by the first and second magnetic field generating means have substantially anti-parallel directions. The present invention furthermore provides a method for attracting and repelling magnetic or magnetizable objects (22), e.g. magnetic particles, to and from a sensor surface (23).

A magnetic resonance contrast agent has a medium, and a contrast structure dispersed in the medium. The contrast structure comprises a magnetic material arranged to create a local region of a local magnetic field such that nuclear magnetic moments of a material when arranged within said local region precess at a characteristic Larmor frequency about a total magnetic field in the local region while in use, the characteristic Larmor frequency being identifiable with the contrast structure, and the total magnetic field in the local region being a substantially spatially uniform magnetic field.

The present invention provides an appropriately produced magnetic material sensor having a small size.

The magnetic material sensor of this invention includes: a magnetoresistive effect film, formed using magnetic films; a current source, for supplying to the magnetoresistive effect film a current having a magnitude and a direction that can change the magnetization directions of the magnetic films; and a detector, for detecting the resistance of the magnetoresistive effect film.

A magnetic sensor device (300) for sensing magnetic particles (15), the magnetic sensor device (300) comprising a magnetic field generator unit (12) adapted for generating a magnetic field, an excitation signal source (302) adapted for supplying the magnetic field generator unit (12) with a static electric excitation signal, an excitation switch unit (303) adapted for switching between different modes of electrically coupling the excitation signal source (302) to the magnetic field generator unit (12), and a sensing unit (11) adapted for sensing a signal indicative of the presence of the magnetic particles (15) in the generated magnetic field.

The present invention can provide a method of determining the presence, location, quantity, or a combination thereof, of a biological substance, comprising: (a) exposing a sample to a plurality of targeted nanoparticles, where each targeted nanoparticle comprises a paramagnetic nanoparticle conjugated with one or more targeting agents that preferentially bind with the biological substance, under conditions that facilitate binding of the targeting agent to at least one of the one or more biological substances; (b) subjecting the sample to a magnetic field of sufficient strength to induce magnetization of the nanoparticles; (c) measuring a magnetic field of the sample after decreasing the magnetic field applied in step b below a threshold; (d) determining the presence, location, quantity, or a combination thereof, of the one or more biologic substances from the magnetic field measured in step (c).

A sensor device (50) for sensing magnetic particles (15), the sensor device (50) comprising a substrate (25) and a sensing unit (11, 20) provided on and/or in and/or near the substrate (25) and being adapted for sensing a detection signal indicative of the presence of the magnetic particles (15), and a magnetic field control unit (30 to 34) provided off the substrate (25) and being adapted for generating a time-dependent magnetic field for interaction with the magnetic particles (15).