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Electrochemical biosensor for detecting hydrogen peroxide, preparation method and application thereof

The present invention relates to an electrochemical biosensor for detecting hydrogen peroxide, a preparation method and an application thereof. The preparation method comprises the steps of (1) synthesizing the silica-three-dimensional graphene composite material; (2) doping nitrogen and carbon into the synthesized silica-three-dimensional graphene composite material; (3) pretreating a glassy carbon electrode; (4) preparing the glassy carbon electrode modified by the nitrogen-carbon-doped silica-three-dimensional graphene composite material through the dispensing method; (5) putting the glassy carbon electrode into a chloroauric acid solution to prepare gold nanoparticle-nitrogen-carbon-doped silica-three-dimensional graphene glassy carbon electrode through chronoamperometry; (6) dipping the glassy carbon electrode into a double-sulfhydryl-modified GDNA solution and cultivating for 2-4 hours; (7) dipping the composite electrode into a hemin solution for 10-14 hours. The electrochemical biosensor is good in stability and reproducibility, high in specificity and sensitivity, fast and simple and convenient to operate. The electrochemical biosensor can be used for detecting the content of hydrogen peroxide in biological samples, cosmetics and the like.
Owner:广东省汕头市质量计量监督检测所
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