61 results about "Hemin" patented technology

The invention discloses an expression system suitable for short-chain non-specific peroxidase and application of the expression system, and belongs to the technical field of enzyme engineering and genetic engineering. According to the expression system disclosed by the invention, a nucleotide sequence of short-chain non-specific peroxidase with SUMO and 8HIS tags is connected to a pET28a vector to obtain a recombinant vector, and escherichia coli BL21 (DE3) is transformed to obtain a genetically engineered bacterium; genetically engineered bacteria are subjected to two-stage temperature control culture in a ZYM5052 liquid culture medium, and meanwhile, 5ALA is added into the culture medium. According to the invention, an escherichia coli self-induction system is used, so that a shake-flask culture can grow at high density in a short time, the culture medium contains rich nutrient substances, and monitoring of cell density and adding of IPTG are not needed in the expression process. Meanwhile, a heme precursor 5ALA is added into an escherichia coli self-induction culture medium, and finally the high-activity short-chain non-specific peroxidase is obtained.

The invention relates to a hydrogel dressing based on near-infrared light controllable cascade reaction and a preparation method thereof, and belongs to the technical field of hydrogel dressings. The preparation method of the hydrogel dressing based on the near-infrared light controllable cascade reaction comprises the following steps: respectively grafting carboxymethyl-beta-cyclodextrin and L-arginine to carboxymethyl chitosan by adopting an EDC / NHS crosslinking method to prepare cyclodextrin grafted chitosan and L-Arg grafted chitosan; adding a photosensitizer into the cyclodextrin grafted chitosan solution I to obtain a C-C-I inclusion compound solution; adding hemin into the cyclodextrin grafted chitosan solution II to obtain a C-C-H inclusion compound solution; and mixing the oxidized hyaluronic acid solution, the L-Arg grafted chitosan solution, the C-C-I clathrate compound solution and the C-C-H clathrate compound solution. The CLIHO disclosed by the invention can be used for synergistically resisting bacteria through PTT / NO and synchronously adjusting a wound microenvironment, so that the healing speed and the healing quality of infectious chronic wounds are improved.

The present invention relates to a composition, a kit and a detection method for detecting hydrogen peroxide on the basis of self-biotinylation according to the peroxidation activity of a guanine quadruplex, and, more specifically, to a composition, a kit and a detection method for detecting hydrogen peroxide, the composition comprising: i) a guanine quadruplex-hemin complex (G-quadruplex-hemin complex); ii) biotinyl tyramide; and iii) a probe capable of binding to the guanine quadruplex.

The invention discloses a food therapy height increasing method. The food therapy height increasing method comprises the following raw materials: enzymolysis bone calcium, oyster peptide, vitamin C, beta-carotene and hemin chloride. According to the present invention, the components such as the enzymolysis bone calcium, the oyster peptide, the vitamin C, the beta-carotene, the heme chloride and the like, which are beneficial to bone repair and bone growth and development, are extracted so as to meet the bone growth requirements of teenagers and children; the raw materials are processed and prepared into powder, all the components are prepared into the solid beverage in a mixing mode, and the solid beverage can be taken by adding sugar or honey or other flavors into water when being eaten. In conclusion, the solid beverage is suitable for teenagers and children to eat, different from a traditional food therapy mode, various growth promoting requirements are met, cooking is not needed, and the solid beverage can be taken after being mixed with water and is convenient to eat.

The invention provides a nano-enzyme with sterilization, tooth desensitization and remineralization functions as well as a preparation method and application thereof, and relates to the technical field of nano-enzymes. Berberine, hemin and calcium fluoride are used as raw materials and are self-assembled into the multifunctional BHCF nano-enzyme with sterilization, tooth desensitization and remineralization functions through multi-dimensional interaction of ionic bonds, Ca < 2 + > coordination bonds, pi-pi accumulation and hydrogen bonds. The BHCF nano-enzyme has a clear peroxidase-like and superoxide dismutase-like double-enzyme catalytic capability and a photodynamic effect, the antibacterial activity of the BHCF nano-enzyme is obviously activated under an illumination condition, and the sterilization rate can reach 99.995%; meanwhile, the BHCF nano-enzyme has a good immediate and lasting sealing effect, and has excellent acid resistance, wear resistance and remineralization capacity. The BHCF nano-enzyme provided by the invention has huge application potential in preparation of drugs for treating periodontitis.

The invention relates to the technical field of heme purification, in particular to a heme extraction and purification method. Comprising the following steps: taking fresh animal blood, centrifugally collecting lower red blood cell precipitate, adding normal saline, uniformly mixing and washing; adding an ethanol solution with the concentration of 1-10%, uniformly mixing, and ultrasonically crushing for 15-60 seconds under the power of 300-800W to obtain erythrocyte crushing liquid; adding an acidic acetone solution into the erythrocyte crushing liquid, uniformly mixing, extracting at normal temperature, centrifuging, and collecting supernate to obtain an acidic acetone extracting solution; slowly adding a NaOH solution to adjust the pH value; adding sodium acetate, stirring, standing, taking precipitate, washing and drying to obtain a crude product; filling a silica gel column; loading the crude product, eluting with an eluent, and collecting an elution component containing a target product; the eluent comprises dichloromethane, methanol and formic acid; and removing the mobile phase in the elution component. The method has the advantages of simple process, low cost, environmental friendliness and capability of obtaining a high-purity product.

This invention belongs to the field of biomaterial preparation technology, specifically disclosing a self-assembled supramolecular peptide nanozyme material, its preparation method, and its application. The preparation method includes the following steps: S1, dissolving β-alanyl-L-histidine in ultrapure water and stirring until homogeneous to obtain solution A; S2, dissolving heme chloride in dimethyl sulfoxide and stirring until homogeneous to obtain solution B; S3, mixing solution A and solution B until homogeneous, adjusting the pH, heating in a water bath, incubating at room temperature, centrifuging, discarding the precipitate, and retaining the supernatant; S4, lyophilizing and grinding the supernatant obtained in S3 to obtain the self-assembled supramolecular peptide nanozyme material. This invention discloses a self-assembled supramolecular peptide nanozyme material, its preparation method, and its application. This supramolecular peptide nanozyme, when applied to food quality and safety testing, can achieve visual and sensitive colorimetric detection of biogenic amines in spoiled food.

The application relates to a kind of nanocomposites, comprising: carrier;Silver;Vancomycin (Van);And hemin chloride (Hemin);Wherein, the silver, vancomycin (Van) and hemin chloride (Hemin) are loaded on carrier;Wherein the nanocomposite releases silver ion, vancomycin and hemin chloride under near infrared light irradiation.The nanocomposite of the application uses nanomaterial as carrier, and can achieve the purpose of high-efficiency sterilization by the loading of silver combined with Van in hollow cavity.At the same time, hemin chloride can realize photo-thermal conversion in cooperation with silver, and effectively remove redundant active oxygen.The successful implementation of the nanocomposite will lay a foundation for constructing efficient and safe antibacterial strategy and provide a new idea for pathogen infection treatment.

The invention discloses a biochemical sensing system based on a catalytic hairpin assembly circular reaction and a G quadruplex-heme DNA enzyme formation reaction. The biochemical sensing system is used for photoelectrochemical colorimetric detection of circular RNA (circRNA). After the nucleic acid probe H1 is combined with the nano magnetic beads (MNPs), the circRNA is specifically recognized, and an MNPs / H1 / circRNA compound is formed; the MNPs / H1 / H2 compound reacts with a probe H2 to form an MNPs / H1 / H2 compound; the H1 probe and the H2 probe release a part of sequences by utilizing restriction endonuclease cutting, and the unreleased sequences are combined with hemin to form G quadruplex-heme DNA enzyme with peroxidase-like activity; through cascade catalytic reaction, the color of a solution of a reaction system is changed, the linear relation between the absorbance of the solution and the concentration of circRNA in the solution is fitted, and an effective method for carrying out photoelectrochemical colorimetric detection on circRNA is developed.

The invention relates to the technical field of biochemical sensing, in particular to a colorimetric and photo-thermal sensor and a preparation method and application thereof. The sensor comprises a target detection object specific capture antibody, Y-DNA (Y-deoxyribonucleic acid), linker-DNA, hemin, dopamine and H2O2 (hydrogen peroxide), one end of the Y-DNA contains a target detection object aptamer sequence, and the other two ends of the Y-DNA are connected with the linker-DNA; the Y-DNA is composed of Y1, Y2 and Y3, and the linker-DNA comprises a G4 sequence; after a target detection object captured by the antibody is combined with an aptamer sequence at one end of Y-DNA, co-incubating with linker-DNA and hemin to form micro-hydrogel; the micro-hydrogel catalyzes dopamine into polydopamine under the condition of H2O2, and a solution obtained through co-incubation is the colorimetric and photo-thermal sensor. The colorimetric photo-thermal sensor realizes visual and high-sensitivity detection of the tumor marker PSA.

The application discloses a micro electrochemical sensor for detecting amyloid polypeptide A beta 1‑42 and a preparation method and application thereof. The preparation method comprises the following steps: taking an acupuncture needle as a bare electrode, sequentially modifying tetrachloroauric acid trihydrate-gold nanoparticles, single-wall carbon nanotubes, hemin, amyloid polypeptide A beta 1‑42 template molecules, mixed polydopamine and ion liquid to form an imprinting polymer, and then eluting to obtain the micro electrochemical sensor ANME / / hemin / / SMIP electrode. The micro electrochemical sensor can be used for high-sensitivity detection of amyloid polypeptide A beta 1‑42 , and realizes specific detection of amyloid polypeptide A beta 1‑42 .

The invention discloses a preparation method of an antibacterial hydrogel dressing loaded with a photo-thermal response functional nano-enzyme and a product application of the antibacterial hydrogel dressing, and belongs to the technical field of medical dressings. The method comprises the following steps: loading heme with high chemical dynamic activity into a lysozyme protein carrier through a solvent removal method to obtain lysozyme-heme nanoparticles LH NPs; in-situ modification of silver is carried out on lysozyme-heme nanoparticles (LH NPs) prepared in the step (1) by using ascorbic acid to obtain silver-modified lysozyme-heme nanoparticles (LHS NPs), so that the prepared nano-composite has chemical dynamic activity and photo-thermal dynamic activity at the same time; the method comprises the following steps: dissolving gelatin powder at high temperature to obtain a pre-gel solution A; an ammonium persulfate initiator is used for inducing propionamide to polymerize, and a pre-gel solution B is obtained; and uniformly mixing the pre-sol A, the pre-sol B and the nano-composite prepared in the step (2), and inducing under an infrared lamp. The product provided by the invention has high chemical power activity and photo-thermal power at the same time.

The invention relates to a homogeneous chemiluminescence immunodetection method based on nucleic acid nanometer machine signal amplification. A detection solution of the chemiluminescence immunodetection method comprises a pair of immunoaffinity probes (an antibody 1-DNA1 and an antibody 2-DNA2), a double-stranded DNA probe (a closed chain / promoter chain), a DNA nano machine and a hemin dimer nucleic acid probe (hemin-DNA3 / hemin-DNA4). When the target protein exists, the target protein can be identified by the immunoaffinity probe sandwich to form an antibody 1-DNA1 / target protein / antibody 2-DNA2 compound, DNA1 and DNA2 are hybridized and subjected to a chain substitution reaction with the double-chain DNA probe based on an ortho-position effect, a promoter chain is released, a cascade hybridization reaction on a DNA nano machine is triggered, a large number of primer chains are released, and the target protein can be identified by the immunoaffinity probe sandwich to form an antibody 1-DNA1 / target protein / antibody 2-DNA2 compound. The primer chain and the hemin dimer nucleic acid probe are subjected to chain substitution reaction, and hemin monomer nucleic acid (hemin-DNA3 and hemin-DNA4 / primer chain) is generated. The hemin monomer nucleic acids have good peroxidase activity and can catalyze an L012-imidazole-hydrogen peroxide system to generate a chemiluminescence signal, so that quantitative analysis of target protein is realized. The immunodetection method is simple to operate, sensitive and high in universality, and has a good clinical application prospect.

To provide a culture medium suitable for culturing and detecting lactic acid bacteria causing deterioration of a beer-like sparkling beverage, and to provide a method for detecting beer-spoilage lactic acid bacteria of the beer-like sparkling beverage by using the culture medium.SOLUTION: The culture medium and the beer-like sparkling beverage are used for detecting beer-spoilage lactic acid bacteria, and the beer-spoilage lactic acid bacteria are detected by inoculating the beer-spoilage lactic acid bacteria into the culture medium and culturing the beer-spoilage lactic acid bacteria in the culture medium, and Lactobacillus paracoinoides or Lactobacillus lindneri is detected by inoculating the beer-like sparkling beverage into the culture medium and culturing the beer-spoilage lactic acid bacteria in the culture medium. K1, To provide a method for detecting beer-spoilage lactic acid bacteria.SELECTED DRAWING: None

This invention discloses a nano-formulation HBMn-FA, its preparation method, and its application. The method includes: dissolving and dispersing a heme chloride alkaline solution in a PLGA / PLGA-PEG / DCM solution and sonicating it to obtain a mixed emulsion A; dissolving and dispersing an aqueous MnCl2 solution and an aqueous BSO solution in a PLGA / PLGA-PEG / DCM solution and sonicating them to obtain a mixed emulsion B; mixing the mixed emulsion A and emulsion B and sonicating them, then adding an aqueous serum albumin (BSA) solution and sonicating, stirring, centrifuging to collect microparticles, redissolving them in deionized water, adding polyethyleneimine and stirring, then adding DSPE-PEG-FA dropwise and stirring, centrifuging, and washing to obtain the nano-formulation HBMn-FA. This formulation can effectively exert ferroptosis, generate toxic ROS, trigger mitochondrial DNA release, and achieve endogenous signal specific activation of the STING pathway to exert anti-tumor therapeutic effects.

The invention provides a multicolor colorimetric detection method of vibrio parahaemolyticus and application of the multicolor colorimetric detection method. The multi-colorimetric detection method comprises the following steps: carrying out catalytic oxidation on TMB (Tetramethylbenzidine) by adopting G-quadruplex DNAzyme to obtain TMB < 2 + >, etching a gold nanorod by the TMB < 2 + > to obtain TMB < 2 + >-AuNRs, and carrying out multi-colorimetric immunodetection on the vibrio parahaemolyticus; the G-quadruplex DNAzyme has peroxidase-like activity, and is obtained by combining a G-quadruplex mediated by a recombinase polymerase with hemin, and the G-quadruplex is used as a carrier of the recombinant polymerase. By adopting the technical scheme provided by the invention to detect the vibrio parahaemolyticus, the method has the advantages of high sensitivity, strong specificity and simplicity and convenience in operation, can realize visual detection, and is convenient and quick; particularly, the method can be applied to food safety detection, so that not only can the detection accuracy be improved, but also the detection time can be greatly shortened.

The present application relates to a kind of intestinal absorption hemin, belong to biological medicine technical field, the intestinal absorption hemin, including hemin and gamma-cyclodextrin;The molar ratio of hemin and cyclodextrin is 1:2;The preparation method of the intestinal absorption hemin, comprising the following steps: (1) hemin is mixed with ammonia water, to obtain hemin solution;Cyclodextrin is mixed with water, to obtain cyclodextrin aqueous solution;(2) the hemin solution is mixed with the cyclodextrin aqueous solution, to obtain mixed solution;(3) the mixed solution is stirred, and dried for 5h, to obtain intestinal absorption hemin.The intestinal absorption hemin prepared in the present application has the advantages of water solubility and stability, high bioavailability after gastrointestinal digestion, and can be used as a raw material for preparing nutritional fortifier for iron supplementation.

The present application relates to a kind of functional gene detection method, specifically sulfate-reducing bacteria metabolic enzyme functional gene detection method.The specific sequence of three different hairpin DNA chains is designed according to the specificity of sulfate-reducing bacteria metabolic enzyme, after mixing three different hairpin DNA chains, incubation is carried out by adding the sample to be detected, after incubation, hemin, TMB and H2O2 are added to the system to react, and the key metabolic enzyme DNA of sulfate-reducing bacteria in the sample to be measured is qualitatively / quantitatively detected by color reaction.The preparation method of the present application is simple to operate, and the reaction is rapid, and visible to naked eye.The construction of sulfate-reducing bacteria metabolic enzyme functional gene detection method has potential application value in the research fields of molecular biology, biological analytical chemistry and medical detection.

The application discloses a red blood cell drug delivery system of a modified indocyanine green derivative dye, the drug delivery system is loaded with a photosensitizer IR808 on the surface of red blood cells and is wrapped with hematin chloride in the interior of the red blood cells. The application also discloses a use of the near-infrared light response red blood cell drug delivery system of the modified indocyanine green derivative dye in preparation of a drug for treating periodontitis. The drug delivery system can be used for PDT anti-infection treatment in the assistance of NIR laser according to the infection condition of a clinical periodontal disease, and can promote the release of hemin and eliminate potential intracellular bacterial infection. The drug delivery system can be used for intrabag administration in a periodontal pocket according to the clinical requirement of different stages of periodontitis, so that a patient can be free from the trauma of periodontal surgery.

The invention relates to the technical field of environmental materials, and discloses an iodide ion monitoring material and a preparation method and application thereof.The preparation method comprises the following steps that a zinc nitrate solution and a hemin solution are mixed, hexadecyl trimethyl ammonium bromide is added, centrifugation is conducted, and Zn Hemin MOFs is obtained; adding ammonia water into the silver nitrate solution, and stirring until the system is colorless and transparent to obtain an intermediate reaction solution; and adding Zn-hemin MOFs into the intermediate reaction solution, carrying out ultrasonic treatment, adding a NaOH solution, standing in a dark place, and centrifuging to obtain the iodide ion monitoring material. According to the iodide ion monitoring material provided by the invention, an Ag site is used as a main capture center of iodide ions, a Zn-Hemin MOF skeleton promotes dispersion and enhanced reactivity of Ag2O nanoparticles, and abundant available active adsorption sites on the surface of Ag2O / Zn-Hemin MOF show excellent I <-> removal capacity.

The invention discloses screening of a Cronobacter sakazakii aptamer and development of a functional nucleic acid double-recognition element biosensor. The screening comprises the following steps: (1) determining a sequence of the Cronobacter sakazakii aptamer; (2) optimizing reaction conditions of the cronobacter sakazakii biosensor; and (3) carrying out visual detection on the cronobacter sakazakii. The detection principle of the sensor is as follows: an aptamer is coupled to a carboxyl magnetic bead to serve as a capture element, enrichment of target bacterial cells is realized through magnetic separation, and a large amount of dissolved contents provide a target with a higher order of magnitude for ribozyme cutting by splitting the target bacterial cells. Short-chain nucleic acid generated after ribozyme cutting is used as a primer of RCA amplification reaction, generation of a product with a large number of G-quadruplex sequences can be triggered, and finally, TMB is catalyzed to generate chromogenic reaction by utilizing peroxidase-like activity of a G-quadruplex / hemin compound, so that visual output of signals can be realized, and ultra-sensitive detection of cronobacter sakazakii is completed.

The invention discloses an application of curcumin in preparation of a neuroprotective drug for inhibiting ferroptosis, and relates to the technical field of medical application of natural drugs, curcumin is used as an active ingredient for preventing or treating nerve cell injury related to ferroptosis, and comprises an H2O2-induced oxidative stress model and a hemin-induced hemorrhagic stroke model; the optimal window of curcumin in a specific concentration range is defined, efficient and safe neuroprotection is achieved, the high-concentration toxicity risk is avoided, the medication accuracy is improved, curcumin can play a multi-target role, the active oxygen level and lipid peroxidation products in cells can be reduced in a concentration-dependent mode, the glutathione content is up-regulated, and the neuroprotective effect is achieved. According to the curcumin, the expression of GPX4 and xCT key proteins is enhanced, so that the ferroptosis process is synergistically inhibited, the neuroprotection theory of the curcumin is enriched, and a solid foundation is laid for clinical application of the curcumin in diseases such as Alzheimer's disease.

The invention discloses a DNA (deoxyribonucleic acid) functionalized nanopore sensor based on nanobubbles, a preparation method of the DNA functionalized nanopore sensor and application of the DNA functionalized nanopore sensor in miRNA (micro Ribonucleic Acid) detection. The sensor comprises a conical nanopore, 3-aminopropyltriethoxysilane, a DNA nanostructure and hemin, wherein the conical nanopore is formed in a borosilicate glass tube, and the 3-aminopropyltriethoxysilane, the DNA nanostructure and the hemin are sequentially modified in the nanopore. The DNA nano structure is a hairpin structure, contains a target recognition sequence and a nucleic acid sequence rich in guanine, and can form a G-quadruplex / hemin compound with hemin after being combined with a target so as to generate oxygen nano bubbles. The sensor takes the nanobubbles as a signal output means, nanobubble pollution of an electrochemical reaction interface is effectively avoided, target detection is realized by performing electrochemical pulse counting on the nanobubbles, and the sensor has the advantages of low background noise, high efficiency, high sensitivity and the like. In addition, the nanopore sensor is low in cost, simple to operate and suitable for popularization and application.

The invention discloses a method for detecting in-situ release of RNA by exosome based on rolling circle replication amplification, and belongs to the technical field of exosome detection. The invention designs and develops a detection probe taking a sulfydryl hairpin probe and a Padlock probe as core components, the sulfydryl hairpin probe can target an exosome membrane protein CD63, and after substances such as the Padlock probe and Phi 29 DNA polymerase are added, an RCA amplification reaction is initiated. Triton X-100 can punch an exosome membrane so that the exosome membrane can release miRNA located in the exosome membrane, and the miRNA is combined with an amplification chain to release a hidden G4 chain body sequence. The added Hemin is combined with the G4 to form a G4 conjoined compound, and the compound can display peroxidase catalytic activity and can catalyze H2O2 to be decomposed to generate oxygen free radicals, so that a detectable signal is generated. The detection method provided by the invention can be used for carrying out high-sensitivity detection on the low-concentration exosome miRNA.

The invention relates to the technical field of lead ion detection, in particular to a lead ion detection colorimetric sensor and application thereof.The lead ion detection colorimetric sensor comprises hemin, a substrate chain S, an enzyme chain Dz and a DNA chain DS with three palindromic structures; in the absence of Pb < 2 + >, the substrate chain S and the enzyme chain Dz are hybridized to form a stable S-Dz double chain; when Pb is introduced, an enzyme chain Dz can be activated, and lead ions are specifically recognized and used as cofactors. Through an annealing process, the DNA chain DS is subjected to intramolecular hybridization and self-folding so as to be self-assembled into a definitely-defined and monodisperse DNA nanosphere; the substrate chain S and the enzyme chain Dz can be hybridized to form a stable S-Dz double chain; a plurality of S-Dz are concentrated on a limited nanosphere surface (DS-S-Dz), the catalysis direction is optimized, side reactions are reduced, and a signal DNAzyme can be quickly formed, so that a color signal is remarkably amplified, and lead ion detection with higher sensitivity, lower background and higher stability is realized.

The application provides a molybdenum-based polyoxometalate-hemin composite nanomaterial and a preparation method and application thereof, and the method comprises the following steps: under room temperature conditions, molybdenum-based polyoxometalate and hemin are mixed by means of manual grinding, and then the obtained mixture is sequentially subjected to centrifugal separation, washing and vacuum drying, so that a solid-phase molybdenum-based polyoxometalate-hemin composite nanomaterial is prepared. 12 The nanocomposite material is synthesized by a simple manual grinding method, and is prepared through the steps of room temperature grinding, centrifugal separation, washing and vacuum drying without the need of complex equipment, high temperature and high pressure or toxic organic solvents. 12 The colorimetric sensor constructed by the nanocomposite material can rapidly and accurately quantitatively detect the AA content in fresh fruits, and has a wide application prospect in the fields of food nutrition analysis, quality control and on-site detection.

This invention belongs to the field of nanobiosensing technology, specifically relating to a programmable perforated nanozyme probe, its preparation method, and its application. The programmable perforated nanozyme probe comprises a metal-organic framework (MOF) material, within which heme chloride is loaded. The MOF material is modified with polydopamine, and platinum nanoparticles are loaded on its surface. The preparation method includes: adding heme chloride and cobalt nitrate hexahydrate to a sodium dodecyl sulfate solution for reaction, followed by the addition of 2-methylimidazole solution for further reaction; centrifuging to collect the product after the reaction to obtain Hemin@ZIF; preparing solutions of Hemin@ZIF and dopamine separately; adding ethanolamine solution to the Hemin@ZIF solution, followed by the addition of dopamine solution; stirring and reacting; centrifuging to collect the product to obtain Hemin@ZIF@PDA; mixing Hemin@ZIF@PDA with a platinum nanoparticle dispersion; stirring and reacting; and centrifuging to collect the product to obtain Hemin@ZIF@PDA@Pt. This invention has significant advantages such as extremely high sensitivity, strong platform versatility, rapid detection adaptation, result visualization, and low cost and stability.

The application belongs to the technical field of hematin quantitative detection, and specifically discloses application of methylene blue cyclopropyl formylated derivative as a chromogenic substrate for detecting hematin, and uses methylene blue cyclopropyl formylated derivative AMB as a chromogenic substrate for detecting hematin Hemin; compared with a classical substrate TMB, the methylene blue cyclopropyl formylated derivative AMB of the application has higher sensitivity in the detection of hematin Hemin; in addition, the amino protons of the methylene blue cyclopropyl formylated derivative AMB of the application are fully substituted by methyl, which inhibits the participation of active oxygen free radicals in oxidation, but is still sensitive to the oxidation pathway of ferric free radicals, has better selectivity compared with TMB, and the AMB system can effectively detect hematin Hemin in a serum sample. Therefore, it can be said that for the detection of hematin Hemin, AMB is a more excellent colorimetric substrate than TMB.

The application provides an iron supplement tabletting candy and a preparation method thereof, and the iron supplement tabletting candy comprises the following components in parts by weight: sorbitol 48-53 parts, hemoglobin peptide 35-40 parts, needle cherry fruit powder 4.1-4.8 parts, hemin chloride 1.2-1.5 parts, L-ascorbic acid 1.5-3.0 parts, and magnesium stearate 3.4-3.8 parts. The tabletting candy and the preparation method improve the viscosity of the mixture by using hemoglobin peptide in the preparation of the hemin tabletting candy, and the flowability and compressibility of the material can be improved by adding the flow agent powder in batches and premixing the material except part of the magnesium stearate and the hemoglobin peptide.

This invention discloses an intelligent chemiluminescence sensing system, a method for determining aflatoxin B1, and its application in traditional Chinese medicine. The system comprises: a complex formed by the binding of the aflatoxin B1 aptamer Apt and a partially complementary cDNA sequence of Apt, which releases the cDNA in the presence of aflatoxin B1; a complex composed of hairpin DNA that can be opened by the released cDNA and two hemin molecules that bind to both ends of the hairpin DNA and quench each other; and an endonuclease whose recognition site is located on the double-stranded DNA formed by the binding of cDNA and hairpin DNA, capable of cleaving the complex B into two hemin-labeled sequences and releasing the cDNA. This system can detect aflatoxin B1 with high sensitivity, accuracy, and speed without washing and drying, and is effective and accurate in the detection of aflatoxin B1 in traditional Chinese medicine, representing a new technology and method for effectively serving the quality control of traditional Chinese medicine.