78 results about "Indocyanine green" patented technology

The invention discloses a water-soluble fibroin-novel indole cyan green nano particle and a preparation method and application thereof, biocompatible silk fibroin and small photo-thermal molecules of novel indole cyan green dye are integrated together, and the water-soluble fibroin-novel indole cyan green nano particle expressed as SF (at) IR820 is obtained. The SF (at) IR820 prepared by the invention can go deep into tumor cells. The SF (at) IR820 can effectively eliminate 4T1 tumor cells in vitro and inhibit tumor growth in vivo when exposed to 808nm laser radiation, a promising strategy is provided for developing an efficient photothermal agent for cancer treatment, and the SF (at) IR820 has certain reference significance and application value.

The utility model discloses an indocyanine green precise puncture and injection device under a laparoscope, which belongs to the field of medical instruments and comprises a needle tube outer cylinder, a middle propelling device arranged in the needle tube outer cylinder, an internal propelling device arranged in the middle propelling device, a negative pressure drainage piece arranged at the head of the needle tube outer cylinder, and a negative pressure drainage piece arranged at the tail of the needle tube outer cylinder. An outer hose is arranged at the other end of the negative pressure drainage piece, a suction cup head is arranged at the other end of the outer hose, an inner hose is arranged at the head of the middle propelling device, the inner hose penetrates through the negative pressure drainage piece and then extends into the outer hose, an injection needle is arranged at the head of the inner hose, and an electrocoagulation wire is arranged in the suction cup head and connected with the injection needle. According to the utility model, an operator can accurately complete puncture and injection operations under a laparoscope; the problem that when indocyanine green is injected, a needle is withdrawn and after the needle is withdrawn, the medicament overflows to pollute the tissue surface is solved; electric coagulation hemostasis can be implemented in the whole operation process; and the medicine injection dosage can be controlled more accurately.

The invention discloses a ZIF-8 composite material loaded with SSZ and ICG as well as a preparation method and application thereof, and belongs to the field of nano medicine. The preparation method comprises the following steps: mixing salazosulfapyridine, indocyanine green and zinc nitrate hexahydrate to obtain a mixed solution; adding 2-methylimidazole into the mixed solution, and stirring to generate ZIF-8 nanoparticles loaded with salazosulfapyridine and indocyanine green; removing uncombined impurities in the ZIF-8 nano-particles by means of centrifugation and washing, so as to obtain the ZIF-8 composite material loaded with SSZ and ICG; in the ZIF-8 synthesis process, indocyanine green and salazosulfapyridine are entrapped at the same time through a co-dissolution method, and compared with traditional'post-loading 'or'multi-step entrapment', the technological process is greatly simplified, and the drug entrapment efficiency and structural stability are improved; meanwhile, the composite material has good biocompatibility and enhanced cytotoxicity in breast cancer cells, and a novel efficient strategy is provided for combined treatment of breast cancer.

The invention discloses a near-infrared fluorescent probe based on magnesium boride, a probe product as well as a preparation method and application of the near-infrared fluorescent probe. The near-infrared fluorescent probe based on magnesium boride is of a pH response type and is prepared by a method comprising the following preparation steps: (1) completely dissolving indocyanine green and polyvinylpyrrolidone in a solution to obtain a mixed solution; (2) keeping the mixed solution obtained in the step (1) away from light and continuously mixing for preset time; (3) adding magnesium boride into the system in the step (2), and continuously mixing the system in a preset time under a dark condition, so that the magnesium boride is combined with the indocyanine green and the polyvinylpyrrolidone; (4) centrifuging the solution obtained in the step (3) at 200-300rmp, and taking supernate; then, centrifuging the supernate at the speed of more than or equal to 12,000 rmp, and taking precipitate; and (5) obtaining a powder product from the precipitate obtained in the step (4), thereby obtaining the near-infrared fluorescent probe based on magnesium boride.

The present application relates to an antifungal microneedle patch and its preparation method and application, the antifungal microneedle patch includes backing layer and the needle head arranged on the surface of the backing layer;The needle head constitutes n×n array on the surface of the backing layer;The surface of the needle head includes fatty acid coating containing antibacterial drug;The inside of the needle head includes photosensitizer;The n is selected from integer.The antibacterial drug is lipophilic triazole antifungal drug.The fatty acid is selected from saturated fatty acid containing 6-12 carbon atoms.The present application innovatively combines antifungal drug itraconazole and photo-thermal agent / photosensitizer indocyanine green, loads in microneedle, can carry out painless minimally invasive local administration, through the antibacterial action, photo-thermal effect and photodynamic effect of antifungal drug released under the excitation of near-infrared light, realize the effective killing to fungi, and will not cause irreversible damage to normal tissue.

The application discloses a kind of nanomotor composite material with eccentric core-echinus shell structure and its preparation method and application, the nanomotor composite material has core-shell structure, its core is made of carbon material, it is filled between core-shell by indocyanine green and staphylolytic enzyme material and is constituted, shell is SiO2@carbon material and is constituted, with echinus porous structure, and indocyanine green and staphylolytic enzyme are loaded on surface;The core of the shell does not coincide with the ball center.The nanomotor composite material of the application is irradiated under the NIR, and it is combined with photosensitizer ICG and carbon material in material, and its asymmetric eccentric structure can make composite material uneven heating, produce self-heating driving force, realize staphylolytic enzyme and ROS for MRSA biofilm targeted action.The nanomotor is applied to preparation of drug for promoting diabetic wound healing, has excellent tissue penetration capacity and drug efficiency, and shows efficient synergistic antibacterial performance.

The application relates to a synthesis method of red luminescent carbon dots with a targeting diagnosis and treatment potential of hepatocellular carcinoma, which comprises the following steps: 1) adding 5-fluorouracil and indocyanine green into deionized water, uniformly mixing, and forming a uniform mixed solution; 2) heating the mixed solution obtained in the step 1) at 160-200 DEG C for 5-9 h, and cooling to room temperature; 3) filtering and dialyzing the solution obtained in the step 2), and obtaining a pure 5-FICD solution; and 4) freeze-drying the solution obtained in the step 3), and obtaining the product. The method takes anticancer drug 5-fluorouracil and photosensitizer indocyanine green as precursors, synthesizes a low-toxicity red light carbon dot through a simple hydrothermal method, improves the shortcoming that a chemotherapy drug has a large side effect, and realizes the targeted killing of hepatocellular carcinoma cells.

The invention discloses a load ICGamp. The invention discloses ZIF-8 nano-particles of CBF, and belongs to the technical field of preparation of nano-drug particles. The multifunctional nanoparticles are formed by simultaneously encapsulating indocyanine green (ICG) and cinobufagin (CBF) components by ZIF-8, and the drug loading capacities of the two drugs are both not lower than 5%. The preparation method comprises the following steps: slowly adding a solution containing cinobufagin and zinc nitrate into a mixed solution containing indocyanine green, bovine serum albumin and 2-methylimidazole, and carrying out centrifugal filtration, washing and drying on a reaction product to obtain the cinobufagin zinc oxide. The multifunctional nano-particles prepared by a one-step synthesis method are uniform in particle size and good in stability, indocyanine green and cinobufagin are uniformly distributed in a carrier, the multifunctional nano-particles have pH and near-infrared light dual response drug release characteristics, triple synergy of chemotherapy, photothermal therapy and photodynamic therapy can be realized, and the multifunctional nano-particles are simple in preparation process and suitable for popularization and application.

This invention discloses a multifunctional tumor-targeted cryotherapy nanoplatform and its applications, belonging to the field of nanobiomedicine technology. The multifunctional tumor-targeted cryotherapy nanoplatform of this invention uses two-dimensional Ti3C2 nanomaterials as a carrier, loaded with indocyanine green as a photosensitizer, surface-modified with polydopamine to improve stability, and loaded with gambogeylic acid as an anti-tumor drug and a heat shock protein inhibitor. It is covalently coupled with nucleic acid aptamers that can recognize cell surface-specific proteins. The multifunctional tumor-targeted cryotherapy nanoplatform has small particle size, high dispersion, and good biocompatibility, and exhibits excellent photothermal conversion ability, photodynamic therapy effect, and chemotherapy effect in tumor-targeted therapy. It can effectively induce tumor cell apoptosis under relatively low temperature conditions, significantly reducing the damage of high temperature to surrounding healthy tissues. It also has the ability to specifically recognize tumor lesions, achieving high enrichment at the tumor site and enhancing the therapeutic effect.

The invention belongs to the technical field of antibacterial photodynamics, and provides antibacterial photodynamics for treating periodontitis by regulating ROS (reactive oxygen species) through microenvironment response and a preparation method of the antibacterial photodynamics. Comprising the following steps: preparing tin sulfide oxide by taking sodium dodecyl benzene sulfonate, tin chloride pentahydrate and L-cysteine as raw materials; zinc nitrate, copper nitrate, urea and thioacetamide are used as raw materials to prepare nano-enzyme; the antibacterial photodynamic material is prepared by taking indocyanine green, bacterial targeting peptide, nano enzyme, tin sulfide oxide and dihydromyricetin as raw materials. The microenvironment response antibacterial photodynamic force aiming at periodontitis infection and treatment characteristics is constructed, the antibacterial photodynamic force is constructed by a strategy of combining bacterial infection microenvironment response ROS programmed regulation and control, precise and long-acting treatment and immune regulation and control, and the curative effect and scene of the antibacterial photodynamic force for treating periodontitis are improved and widened.

The invention relates to a high-sensitivity treatment-free negative image CTP plate material and a preparation method thereof, and belongs to the technical field of CTP plate materials, the treatment-free negative image CTP plate material comprises a plate base and a photosensitive layer, the photosensitive layer contains a modified graphene oxide composite material, the modified graphene oxide composite material can promote rapid conduction of heat, strengthen scratch resistance and improve the sensitivity of the photosensitive layer, and the modified graphene oxide composite material is essentially formed by compounding an infrared absorption dye new indocyanine green onto nitrogen-aluminum co-doped graphene oxide, so that the infrared absorption dye new indocyanine green can be effectively absorbed by the nitrogen-aluminum co-doped graphene oxide; the specific preparation method comprises the following steps: doping nitrogen and aluminum elements in graphene oxide by using urea and aluminum nitrate nonahydrate to prepare nitrogen-aluminum co-doped graphene oxide, and then adding amino-modified new indocyanine green to react by taking dehydration esterification as a reaction principle, thereby obtaining the nitrogen-aluminum co-doped graphene oxide. The amino-modified novel indocyanine green is prepared from a short-carbon-chain diamine compound and novel indocyanine green under the action of amino and halogen.

The application belongs to the technical field of pharmaceutical preparations, and particularly relates to an indocyanine green preparation and a preparation method and application thereof. The preparation is a liquid preparation or a freeze-dried preparation, raw materials for preparing the preparation include indocyanine green, phospholipid, cholesterol, a freeze-drying protective agent, water and a pH regulator, the pH regulator is selected from one or more of sodium citrate, potassium citrate, sodium tartrate, sodium acetate, sodium lactate and sodium succinate, and the pH of the preparation is adjusted to 6.0-8.5. By means of a specific organic acid salt buffer system and a specific preparation process (the freeze-drying protective agent is added before lipid film deposition), the application can specifically inhibit the growth of impurities with RRT 0.7 and RRT 0.3, respectively, and significantly improve the chemical stability of the preparation. Meanwhile, the preparation can realize accurate positioning of sentinel lymph nodes and second and third lymph nodes, and can be used for preparing a lymph tracer. The application effectively solves the technical problems of rapid impurity growth, poor stability and inaccurate multi-stage lymph positioning of the existing ICG preparation.

The invention aims to provide a preparation method and application of a gouty arthritis microenvironment response type diagnosis and treatment integrated nanoprobe, and belongs to the technical field of biomedical nanomaterials. Natural tea extract tea polyphenol is used as a raw material, polyoxyethylene-polyoxypropylene-polyoxyethylene copolymer is used as a template, a soft template method is utilized, and the gouty arthritis microenvironment response type diagnosis and treatment integrated nanoprobe is prepared. And stirring, centrifuging and freeze-drying to synthesize the mesoporous tea polyphenol nano-particles. Meanwhile, indocyanine green with fluorescence emission in a near-infrared second region and a steroid anti-inflammatory drug dexamethasone are connected to the two ends of an active oxygen response type ketal thiol bond respectively, and the active oxygen response type nano-particles are synthesized through stirring, dialysis and freeze drying. And finally, loading the response type nano-particles in mesoporous tea polyphenol by a solvent impregnation method to realize anti-inflammatory and anti-oxidation treatment of gouty arthritis at different stages.

The present invention relates to an enhanced sonodynamic therapeutic agent using a liposome having the ability to transfer oxygen, and more particularly, to lipid nanoparticles having the ability to transfer oxygen in which perfluorocarbon (PFC) and indocyanine green (ICG) are loaded inside liposomes to increase the efficiency of sonodynamic therapy.

The present application provides a kind of hydrogel dressing with photothermal / photodynamic synergistic antibacterial activity and its preparation method, belong to biological medical tissue engineering material technical field, the present application is by the indocyanine green (ICG) with photodynamic (PDT) treatment ability and the hollow prussian blue nanoparticle Pt-HPB with photothermal treatment ability doped with platinum as photosensitizer, combined with the hydrogel made of oxidized sodium alginate and polylysine, these antibacterial nanoparticles are supported by the hydrogel with good water dispersibility, a kind of composite hydrogel (Pt-HPB-BSA & ICG@Gel) with photothermal photodynamic synergistic antibacterial ability is prepared, as the medical dressing of wound infection antibacterial treatment and promoting wound repair.

The invention discloses a near-infrared I / II region orthogonal dual-channel organic fluorescent probe and a preparation method and application thereof, belongs to the technical field of fluorescent probes, and determines a pair of spectrum orthogonal nanoparticles by means of calculation and spectrum guidance: TQx-FTA NPs provides white light excited NIR I channel emission, and TPzCl-FTA NPs provides 980nm excited NIR II blood vessel emission. In the rabbit model, stable co-registration, high signal-to-noise ratio and extremely low crosstalk are realized in the forward and backward processes. The near-infrared I-region / II-region orthogonal dual-channel organic fluorescent probe can accurately position stenosis and blockage and quantify damage-related leakage, and is superior to indocyanine green under matched acquisition setting. Through combination of environment sustainability, batch expandability and predictable spectrum engineering, the long-term obstacle problem of practical double-color navigation is solved.

The invention discloses a composition with a tumor mitochondrial dual regulation effect, temperature-sensitive hydrogel and application, and belongs to the technical field of biological medicine, the composition is composed of a peroxide initiator and a mitochondrial autophagy inhibitor, the peroxide initiator is used for causing mitochondrial damage, and the mitochondrial autophagy inhibitor is used for causing mitochondrial autophagy. The mitochondrial autophagy inhibitor is used for inhibiting mitochondrial The peroxide initiator is indocyanine green, chlorin, iron oxide nanoparticles or Fe-MOFs (Metal-Organic Frameworks); the mitochondrial autophagy inhibitor is chloroquine, 3-methyladenosine or bajulomycin A1; the tumor is specifically melanoma; the combination of the peroxide initiator and the mitochondrial autophagy inhibitor can better inhibit the energy metabolism of tumor cells and inhibit the occurrence and development of tumors, and after the composition is delivered through the temperature-sensitive hydrogel carrier, the treatment effect of the composition on tumors is further improved.

The invention belongs to the technical field of biological medicine, and provides a zingiberone-loaded self-assembled nano-drug, a soluble microneedle patch as well as a preparation method and application thereof, and the zingiberone-loaded self-assembled nano-drug comprises zingiberone, indocyanine green and a polymer mPEG-PLA in a mass ratio of (1.5-5): (0.35-1.4): (10-20). The invention also provides a preparation method of the zingiberone-loaded self-assembled nano-drug. The invention further provides a soluble microneedle patch, the soluble microneedle patch comprises the zingiberone-carrying self-assembled nano-drug, and the soluble microneedle patch comprises a patch substrate and a needle head arranged on the surface of the patch substrate; the needle head is prepared from a ball-carrying zingerone self-assembled nano-drug and a soluble matrix material. The zingiberone-loaded self-assembled nano-drug provided by the invention has an anti-tumor effect, can be used for treating melanoma, and improves the solubility and bioavailability of zingiberone.

The application discloses a kind of based on ferroptosis and photodynamic cooperative treatment targeted laryngeal cancer nanoparticles and its preparation method and application.The nanoparticle is with polylactic acid-glycolic acid copolymer as carrier, co-loading photosensitizer indocyanine green and ferroptosis inducer Erastin, and realizes active targeting by cRGD peptide modification.Under laser irradiation, ICG produces reactive oxygen species and kills tumor cells, and Erastin induces ferroptosis by inhibiting GPX4 pathway, and the synergistic effect significantly enhances the anti-tumor effect.The application solves the problem of PDT hypoxic restriction and drug targeting deficiency, and PDT is combined with ferroptosis through nano-drug delivery platform, and the two promote each other, showing stronger anticancer effect, with high efficiency, stability and safety, suitable for targeted therapy of laryngeal squamous cell carcinoma.

The invention belongs to the technical field of nano materials and biological medicines, and relates to carrier-free self-assembled drug nanoparticles as well as a preparation method and application thereof. The carrier-free self-assembled drug nanoparticle provided by the invention is formed by self-assembling four components, namely a chemotherapeutic drug, a photosensitizer, a biomineralized shell and a cancer cell membrane; wherein the chemotherapeutic drug is doxorubicin hydrochloride, the photosensitizer is indocyanine green, the biomineralized shell is prepared from a potassium permanganate solution, and the cancer cell membrane is a 4T1 cancer cell membrane. The invention also provides a method for preparing the nanoparticles, a drug containing the nanoparticles and application of the nanoparticles in preparation of tumor treatment drugs. The nanoparticle provided by the invention has the characteristics of high drug loading rate, simple preparation and no carrier, and in-vitro cell experiments and animal experiments prove that the nanoparticle has a remarkable tumor treatment effect.

The invention provides a temperature-sensitive hydrogel for resisting bacteria and promoting repair and a preparation method thereof, the temperature-sensitive hydrogel comprises PEG-NB, a matrix metalloproteinase degradable peptide cross-linking agent, gelatin, indocyanine green and levofloxacin, and the temperature-sensitive hydrogel is prepared by photo-crosslinking. According to the temperature-sensitive hydrogel provided by the invention, the structure and the performance of the hydrogel are optimized through the synergistic effect of the gelatin and the PEG-NB, and the mechanical strength, the biocompatibility, the degradability and the like of the hydrogel are remarkably improved; meanwhile, the photo-thermal antibacterial function of the photo-thermal agent indocyanine green and the chemical antibacterial function of levofloxacin are integrated, and near-infrared light irradiation is combined, so that the antibacterial and skin repair promoting effects of the hydrogel are synergistically improved.

This invention discloses a FA-ICG-NBs nanobubble, its preparation method, and its application in the preparation of tumor-targeted therapeutic agents. The FA-ICG-NBs nanobubble employs a composite phospholipid shell, with folic acid (FA) covalently bonded to the surface and indocyanine green (ICG) loaded onto it, encapsulating a mixture of oxygen and sulfur hexafluoride. The preparation method includes: preparing a lipid solution for forming the composite phospholipid shell; mixing the lipid solution with the oxygen and sulfur hexafluoride mixture, and then performing a reciprocating shearing gas-liquid interface self-assembly to obtain the FA-ICG-NBs nanobubble. The FA-ICG-NBs nanobubble prepared by this invention utilizes dual-wavelength photoacoustic imaging for non-invasive assessment of tumor blood oxygenation and hypoxia improvement, combined with ICG fluorescence timing for screening the optimal therapeutic window, and synergistic oxygen-enhanced sonodynamic therapy. It exhibits good biocompatibility, structural stability, and integrates targeted enrichment, blood oxygenation assessment, hypoxia monitoring, timing regulation, and enhanced therapeutic effects.

This invention discloses a wasp venom peptide-M nanocomposite, its preparation method, and its applications, belonging to the field of medicinal chemistry. Using indocyanine green as a morphology mediator, this invention prepares an ultra-small particle size dendritic tetrasulfide by reacting it with triethylamine, hexadecyltrimethylammonium bromide, tetraethyl orthosilicate, and bis[3-(triethoxysilyl)propyl]tetrasulfide in water. Wasp venom peptide-M is then loaded onto this nanocomposite to obtain the wasp venom peptide-M nanocomposite. In vitro experiments have confirmed that this composite can significantly kill 4T1 breast cancer cells. Furthermore, thanks to its ultra-small particle size, it also exhibits good targeting and enrichment, significant anti-tumor efficacy, and excellent safety in a breast cancer brain metastasis model, providing a novel and efficient nanomedicine delivery system for brain tumor treatment and the development of related therapeutic drugs.

The application discloses an AS1411 aptamer functionalized liposome which comprises lipids, indocyanine green, chelidonium red base and AS1411 aptamer. The application also discloses a preparation method and application of the AS1411 aptamer functionalized liposome. The AS1411 aptamer functionalized liposome provided by the application has excellent fluorescence imaging and chemical-photothermal treatment effects, and is expected to become an ideal diagnosis and treatment nano system and has the potential for future clinical application in cancer treatment.

The present application belongs to the technical field of kits, and particularly relates to a detachable kit for parathyroid blood supply detection, which comprises a box body, an openable box cover arranged at the top of the box body, a developing solution injection mechanism, a liquid medicine injection mechanism and a three-way connecting mechanism arranged in the box body, a first end of the three-way connecting mechanism being connected with the developing solution injection mechanism, a second end of the three-way connecting mechanism being connected with the liquid medicine injection mechanism, and a third end of the three-way connecting mechanism being used for connecting a remaining needle, the three-way connecting mechanism being capable of being switched between a developing solution injection function and a reagent injection function. The kit designed in the present application is used in cooperation with developing reagents such as indocyanine green aqueous solution, and can guide a doctor to make a decision on whether to retain or transplant parathyroid glands, or help the doctor to find a position of a parathyroid adenoma to be removed during a total thyroidectomy and central lymph node dissection surgery or a parathyroid adenoma resection surgery. The device can be repeatedly used, material is saved, the environment is protected, mechanical injection is achieved, and efficiency is high.

The invention discloses a choroid blood vessel image generation method and device and terminal equipment, and the method comprises the steps: extracting a choroid blood vessel structure on indocyanine green angiography as a label, training a segmentation model, achieving the effect of generating a high-precision choroid blood vessel image through employing a relatively fuzzy non-invasive fundus image, and improving the detection precision of the choroid blood vessel image. The accuracy of recognizing the blood vessel structure of the choroid based on the noninvasive fundus image is improved, the influence of human errors caused by manual annotation is reduced, and the accuracy of recognizing the blood vessel structure of the choroid based on the noninvasive fundus image is further improved.

The application provides a drug composition based on a two-dimensional halogen bond organic framework XOF and application thereof in treating bacterial keratitis, the drug composition is formed by loading new indocyanine green to form a composite drug based on the two-dimensional halogen bond organic framework XOF, the composite drug has good bacteriostatic effect and can have good treatment effect on bacterial keratitis, and provides a new choice for the treatment of bacterial keratitis, can avoid drug resistance caused by long-term use of fixed drugs such as antibiotics, and has a significant application prospect.

A composition for making a model for simulating soft tissue using indiocyanine green (“ICG”) type fluorescence includes a material in particle form suitable for emitting a fluorescence in the near infrared and including calcium oxide (CaO), copper oxide (CuO), and silicon dioxide (SiO2), or including particles of CaCuO2(SiO2)4. A mixture for making a model for simulating a soft brain tissue using ICG-type fluorescence includes silicone and a composition including a material in particle form suitable for emitting a fluorescence in the near infrared. A model for simulating a soft brain tissue using ICG-type fluorescence includes the mixture.

The utility model discloses a ureteral stent with an indocyanine green coating, and relates to the technical field of ureteral stents, the ureteral stent comprises a ureteral stent body, the ureteral stent body is composed of a pipe stent linear section, a curled section and a spiral supporting section, and the spiral supporting section of a spiral structure is used for conducting auxiliary supporting on the ureter of the human body; the outer sides of the straight line section, the curled section and the spiral supporting section of the ureter frame are coated with indocyanine green coatings, and the indocyanine green coatings are used for emitting light under the specific laser wavelength, so that the visual effect of the ureter frame is achieved. By arranging the indocyanine green coating and utilizing the fluorescence characteristic of indocyanine green, the ureter can be developed in an operation, so that the visualization effect of the ureter is improved, the operation precision can be improved, complications are reduced, the treatment effect of a patient is improved, and in addition, due to the structural arrangement of the spiral supporting section, the structure is simple and convenient. And the smoothness and the stability of the ureter frame in use can be further improved.

The application discloses a preparation method of an indocyanine green dimer. The method comprises the following steps: taking indocyanine green as a starting material, and dimerizing in a solvent under light-proof conditions and in a temperature range of 0-20 DEG C. The method has high conversion rate of target product, simple subsequent collection and purification steps, and can easily obtain high-purity indocyanine green dimer. The method can be used for industrial production of indocyanine green dimer and safety and impurity research of the drug indocyanine green.