The invention discloses a preparation method of a medicinal composition for killing aphids, which comprises the following steps: 1) preparing raw materials; 2) adding water to decoct the petunias, crane grass buds, and thunder pills for two times, and the medicinal liquid for later use; 3) Alisma, Sophora japonica rice, Thousand hammers, Nutmeg, Xu Changqing, Magnolia magnoli, decoct twice, filter, and concentrate the filtrate to a relative density of 1.15; 4) Merge the liquid medicine; 5) Adjust the pH value of the solution; 6) Filter , filled, sterilized, and obtained. The pharmaceutical composition described in the present invention can kill aphids in a targeted manner, has good safety, good biodegradation, no pollution and irritating odor, no environmental toxicity, no residue in animals, and does not destroy the ecological balance. The crop growth environment has little impact; it has good permeability and wetness, interferes with the central nervous system of insects, inhibits the synthesis and development of insect eggs, and is not easy to develop drug resistance; it has good quick effect and is suitable for widespread application in pest control.