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EBV (Epstein-Barr Virus) LMP2A (Latent Membrane Protein 2A) multi-epitope peptide for immunological therapy and application of EBV LMP2A multi-epitope peptide

An Epstein-Barr virus and immunotherapy technology, applied in the field of immunology, can solve the problem of low tumor killing efficiency and achieve high induction efficiency and great application potential

Inactive Publication Date: 2016-03-23
SHENZHEN BEIKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current tumor killing efficiency is still low

Method used

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  • EBV (Epstein-Barr Virus) LMP2A (Latent Membrane Protein 2A) multi-epitope peptide for immunological therapy and application of EBV LMP2A multi-epitope peptide
  • EBV (Epstein-Barr Virus) LMP2A (Latent Membrane Protein 2A) multi-epitope peptide for immunological therapy and application of EBV LMP2A multi-epitope peptide
  • EBV (Epstein-Barr Virus) LMP2A (Latent Membrane Protein 2A) multi-epitope peptide for immunological therapy and application of EBV LMP2A multi-epitope peptide

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Embodiment Construction

[0027] The preferred embodiments of the present invention will be further described in detail below in conjunction with the accompanying drawings.

[0028] 1. In vitro synthesis of Epstein-Barr virus tandem epitope peptides

[0029] In the present invention, two HLA*02:01 type LMP2A epitope peptides in series, LMP2A 356-364 and LMP2A 426-434 The sequence is:

[0030] LMP2A 356-364 : FLYALLALL;

[0031] LMP2A 426-434 : CLGGLLTMV;

[0032] The two are connected in series to obtain the tandem epitope peptide LMP2A 356-364 / 426-434 , the sequence of the obtained LMP2A tandem epitope peptide is:

[0033] LMP2A tandem epitope peptide: FLYALLALLCLGGLLTMV.

[0034] DC cells were loaded with LMP2A tandem epitope peptides.

[0035] The above three epitope peptides were synthesized according to the methods of the prior art, and the tandem epitope peptides of this example were synthesized according to the conventional methods of the prior art. The sequence and molecular weight of...

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Abstract

The invention provides an EBV (Epstein-Barr Virus) LMP2A (Latent Membrane Protein 2A) multi-epitope peptide for an immunological therapy and application of the EBV LMP2A multi-epitope peptide. The EBV LMP2A multi-epitope peptide for the immunological therapy is an amino acid sequence shown in SEQ ID NO.1. By adopting the technical scheme of the invention, after the EBV LMP2A multi-epitope peptide is loaded on DCs (Dendritic Cells) sourced from PB (Peripheral Blood) of a human body, the ripening ability and the presenting ability of the DCs can be more effectively promoted, and more cell factors can be secreted by the DCs; after the DCs on which the EBV LMP2A multi-epitope peptide is loaded are in co-cultivation with CTL (Cytotoxic T Lymphocytes), CTL cells in EBV specificity can be induced, an NPC (Nasopharyngeal Carcinoma) cell line CNE1 can be effectively subjected to targeted killing in vitro and in vivo, and the effect of the EBV LMP2A multi-epitope peptide is better than the effect of independently applying LMP2A356 to 364 or LMP2A426 to 434 or a mixture of the LMP2A356 to 364 and the LMP2A426 to 434; a better direction is provided for immune cell clinical therapy for treating NPC and other diseases related to EBV.

Description

technical field [0001] The invention belongs to the technical field of immunology, and in particular relates to an Epstein-Barr virus LMP2A tandem epitope peptide for immunotherapy and application thereof. Background technique [0002] Epstein-Barr Virus (Epstein-Barr Virus, EBV) is a member of the herpesvirus γ subfamily of lymphotropic viruses, and is the first human virus found to be related to tumorigenesis. The target cells infected by Epstein-Barr virus are mainly B lymphocytes. Recent studies have confirmed that Epstein-Barr virus can also infect human T cells, NK cells, and mononuclear macrophages. Epstein-Barr virus infection is associated with a variety of diseases, such as Burkit lymphoma in African children, nasopharyngeal carcinoma, Hodgkin lymphoma, adult T / NK cell lymphoma, patients treated with various immunosuppressants, and lymphoproliferative disorders in patients with organ transplantation It is closely related to the lymphoma caused by EBV and EBV-rela...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00A61K39/245A61P35/00
CPCC07K14/005A61K39/12A61K2039/5154A61K2039/5158A61K2039/575C07K2319/00C12N2710/16222C12N2710/16234
Inventor 胡祥周欣曾晓萍刘沐芸李陶
Owner SHENZHEN BEIKE BIOTECH
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